##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	SRR19654133_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	34859877
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.60849173392092	32.0	32.0	32.0	32.0	32.0
2	31.692475105405563	32.0	32.0	32.0	32.0	32.0
3	35.729728306270275	37.0	37.0	37.0	32.0	37.0
4	36.35068804172774	37.0	37.0	37.0	37.0	37.0
5	36.43495727193759	37.0	37.0	37.0	37.0	37.0
6	40.09067929298775	41.0	41.0	41.0	37.0	41.0
7	40.10483117883634	41.0	41.0	41.0	37.0	41.0
8	40.244774013402285	41.0	41.0	41.0	37.0	41.0
9	40.21740372176299	41.0	41.0	41.0	41.0	41.0
10-11	40.281722838551616	41.0	41.0	41.0	41.0	41.0
12-13	40.24090495213164	41.0	41.0	41.0	41.0	41.0
14-15	40.27417533630425	41.0	41.0	41.0	41.0	41.0
16-17	40.27596753998874	41.0	41.0	41.0	41.0	41.0
18-19	40.27148995677753	41.0	41.0	41.0	41.0	41.0
20-21	40.20273740495412	41.0	41.0	41.0	41.0	41.0
22-23	40.16174501992649	41.0	41.0	41.0	39.0	41.0
24-25	40.19941562042804	41.0	41.0	41.0	41.0	41.0
26-27	40.17293840422902	41.0	41.0	41.0	41.0	41.0
28-29	40.09258688721134	41.0	41.0	41.0	39.0	41.0
30-31	40.10627782765843	41.0	41.0	41.0	37.0	41.0
32-33	40.11763293083335	41.0	41.0	41.0	39.0	41.0
34-35	40.11243811904443	41.0	41.0	41.0	39.0	41.0
36-37	40.09124573216366	41.0	41.0	41.0	37.0	41.0
38-39	40.068936502558515	41.0	41.0	41.0	37.0	41.0
40-41	40.05493248011173	41.0	41.0	41.0	37.0	41.0
42-43	40.00502467349497	41.0	41.0	41.0	37.0	41.0
44-45	40.020160025234745	41.0	41.0	41.0	37.0	41.0
46-47	40.00740201980632	41.0	41.0	41.0	37.0	41.0
48-49	39.9832487073893	41.0	41.0	41.0	37.0	41.0
50-51	39.916950854416385	41.0	41.0	41.0	37.0	41.0
52-53	39.93492844223174	41.0	41.0	41.0	37.0	41.0
54-55	39.882155221029606	41.0	41.0	41.0	37.0	41.0
56-57	39.880694788452644	41.0	41.0	41.0	37.0	41.0
58-59	39.87277595672526	41.0	41.0	41.0	37.0	41.0
60-61	39.80446805649946	41.0	41.0	41.0	37.0	41.0
62-63	39.76322696147207	41.0	41.0	41.0	37.0	41.0
64-65	39.757508725575825	41.0	41.0	41.0	37.0	41.0
66-67	39.72657252921461	41.0	41.0	41.0	37.0	41.0
68-69	39.64664838031413	41.0	41.0	41.0	37.0	41.0
70-71	39.66807025739075	41.0	41.0	41.0	37.0	41.0
72-73	39.58344402649499	41.0	41.0	41.0	37.0	41.0
74-75	39.6188483252537	41.0	41.0	41.0	37.0	41.0
76-77	39.082784901966235	41.0	39.0	41.0	34.5	41.0
78-79	39.40310761853807	41.0	41.0	41.0	37.0	41.0
80-81	39.6101931598898	41.0	41.0	41.0	37.0	41.0
82-83	39.749046920045075	41.0	41.0	41.0	37.0	41.0
84-85	39.70847156173271	41.0	41.0	41.0	37.0	41.0
86-87	39.73008055937776	41.0	41.0	41.0	37.0	41.0
88-89	39.66950028251678	41.0	41.0	41.0	37.0	41.0
90-91	39.68702851418552	41.0	41.0	41.0	37.0	41.0
92-93	39.64000092427176	41.0	41.0	41.0	37.0	41.0
94-95	39.50261491169346	41.0	41.0	41.0	37.0	41.0
96-97	39.46970372270677	41.0	41.0	41.0	37.0	41.0
98-99	39.525110975004296	41.0	41.0	41.0	37.0	41.0
100-101	38.98554421175955	41.0	39.0	41.0	34.5	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	2.0
15	5.0
16	4.0
17	18.0
18	61.0
19	188.0
20	541.0
21	1547.0
22	3905.0
23	8833.0
24	16809.0
25	29261.0
26	48756.0
27	76002.0
28	110045.0
29	152556.0
30	201221.0
31	254809.0
32	318479.0
33	383723.0
34	460969.0
35	563175.0
36	713355.0
37	969687.0
38	1526845.0
39	3462494.0
40	2.5556587E7
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	41.91390843527499	12.728004583579475	5.91588456730531	39.44220241384023
2	24.982489180899865	14.0602188584888	30.734703969265297	30.22258799134604
3	20.461543797185517	17.29495201603838	22.518544744148123	39.724959442627984
4	23.633764320660216	24.652473376283158	21.780340639313504	29.933421663743122
5	24.319087528622088	29.11640508656987	23.199703200329708	23.364804184478334
6	20.49386749127084	32.0197257150391	24.811685365384392	22.674721428305673
7	16.170653728927388	24.989646406382903	40.114722722630376	18.724977142059338
8	18.77917412043651	24.467401304944364	30.90549344164353	25.8479311329756
9	18.62973472306048	22.956336380845205	33.62792069716198	24.78600819893233
10-11	21.708656065790482	30.917339205198065	24.54607728102683	22.827927447984624
12-13	21.751201946384914	25.26431690006785	27.45648419939223	25.527996954155007
14-15	21.36448875020414	26.14102310229035	27.212915868865515	25.281572278639995
16-17	21.884560984540002	26.62192472351132	26.73156880888181	24.76194548306687
18-19	21.683632935363615	26.864558099854403	26.4823017766425	24.96950718813948
20-21	21.875825091644337	26.655415682102724	26.61429807729048	24.854461148962454
22-23	21.80720976152612	26.817263296712145	26.51480525877931	24.860721682982415
24-25	21.76919789144979	26.496632883940368	26.452790215708312	25.28137900890154
26-27	21.637594992087894	26.582873485181835	26.535641534248672	25.243889988481598
28-29	21.686891698373543	26.789548085218527	26.4959177133865	25.027642503021426
30-31	21.818309915436593	26.64683957433355	26.36949063245404	25.165359877775817
32-33	21.809824946360962	26.534812322352174	26.50142799574488	25.153934735541988
34-35	22.131124909259395	26.640404608540535	26.23530180055207	24.993168681648
36-37	21.793517024913786	26.528155577540808	26.262427804961003	25.415899592584406
38-39	21.684734716181193	26.63770416096527	26.466419774492245	25.211141348361295
40-41	21.998407796107713	26.931382707622696	26.22645583428526	24.843753661984326
42-43	22.011566477988435	26.638609482184922	26.237896651213084	25.111927388613566
44-45	22.044289968257143	26.587491086418158	26.312856305709666	25.055362639615037
46-47	21.986916364552968	26.501841024275336	26.427879053201288	25.08336355797041
48-49	21.76617705726783	26.42396190924573	26.30034349413232	25.50951753935412
50-51	21.882784919483054	26.333074544807488	26.544397789710377	25.239742745999084
52-53	22.284886432617075	26.64196577629921	26.05252881414355	25.020618976940167
54-55	22.240808863817115	26.532316927429157	26.039300147299606	25.187574061454125
56-57	21.976965279530337	26.25456409025103	26.50646766361824	25.262002966600395
58-59	21.99974830475045	26.3603297764287	26.584861790076463	25.055060128744387
60-61	22.106588087255965	26.16903786382917	26.48057962953228	25.24379441938258
62-63	21.982156880932816	26.267998595573	26.386664042246867	25.363180481247316
64-65	22.075704430200943	26.523157686016873	26.180476682470484	25.220661201311707
66-67	22.121507122093696	26.24491466254651	26.16446093167489	25.469117283684913
68-69	22.049944538964215	26.418014634919956	26.43652172895218	25.09551909716365
70-71	22.330386453792713	26.661869621056923	26.24913033645504	24.758613588695326
72-73	22.439471724282846	26.168440506588748	26.14717952818643	25.244908240941978
74-75	22.261401323277536	26.073996707261088	26.49801684218613	25.166585127275248
76-77	22.150755150398265	26.39000131870804	26.448614549041583	25.01062898185212
78-79	22.133238609999623	26.32330175362954	26.149123558154947	25.394336078215886
80-81	22.167184580156135	26.368536561148336	26.313828521113397	25.150450337582132
82-83	22.417918772395762	26.54115300589574	26.091748152548238	24.94918006916026
84-85	22.37343826899475	26.399981824355372	26.005496325262456	25.221083581387422
86-87	22.308583481630052	26.206918296137488	26.330122508212778	25.154375714019682
88-89	22.52873824033076	26.237715658002177	26.2140221935716	25.019523908095465
90-91	22.549296253638342	26.066660413878267	26.229371087438263	25.154672245045123
92-93	22.328149833955617	25.99800973360178	26.34723579450227	25.326604637940335
94-95	22.52433188826275	26.10390160163715	26.23341699773055	25.13834951236955
96-97	22.579449763955388	26.012610274062357	26.08330116612074	25.324638795861514
98-99	22.461644212724266	26.219789114760395	26.243225875974407	25.075340796540928
100-101	22.585985015776817	26.34616812806981	26.095111301324298	24.97273555482907
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	103.0
1	77.5
2	42.5
3	33.0
4	41.5
5	58.0
6	71.5
7	88.5
8	130.0
9	151.0
10	161.5
11	206.0
12	257.5
13	293.5
14	342.0
15	407.0
16	472.5
17	572.5
18	739.5
19	976.0
20	1311.5
21	1782.0
22	2689.5
23	3996.5
24	5925.0
25	8988.0
26	13441.5
27	19693.0
28	27597.0
29	39738.5
30	57501.5
31	78937.5
32	110177.5
33	152080.0
34	194942.5
35	249502.5
36	329415.0
37	438684.0
38	576395.0
39	769571.0
40	1009337.5
41	1248824.0
42	1484574.5
43	1722717.0
44	1954706.5
45	2150676.0
46	2274413.0
47	2281629.5
48	2192794.0
49	2057296.5
50	1900821.5
51	1732047.5
52	1565357.0
53	1374230.5
54	1170714.0
55	1011335.5
56	860190.0
57	715314.0
58	582756.0
59	455183.0
60	365275.5
61	327920.5
62	286989.5
63	229806.0
64	180810.0
65	142824.5
66	121672.0
67	99108.5
68	72959.0
69	53282.5
70	38871.5
71	28623.0
72	22629.5
73	18963.5
74	15767.5
75	11403.5
76	6988.5
77	2707.0
78	528.5
79	169.0
80	53.0
81	25.5
82	14.5
83	9.0
84	4.0
85	1.0
86	1.5
87	2.5
88	1.5
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.5
98	0.5
99	1.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03443213525968551
2	0.0
3	0.0
4	0.019070635275047012
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0027194588208099527
10-11	0.0034925539180760737
12-13	0.0016781470571453825
14-15	0.0
16-17	7.67357842369897E-4
18-19	6.310980385845882E-5
20-21	3.241549016366294E-4
22-23	0.0
24-25	1.4343137240558824E-6
26-27	0.0
28-29	1.8215784295509707E-4
30-31	0.0
32-33	0.002020948037194738
34-35	5.952401954831912E-4
36-37	4.044764701837588E-4
38-39	0.0042312254859648525
40-41	5.7372548962235294E-5
42-43	0.0
44-45	0.002451242154411503
46-47	2.022382350918794E-4
48-49	5.278274504525647E-4
50-51	3.155490192922941E-5
52-53	0.0
54-55	8.978803912589823E-4
56-57	3.1411470556823827E-4
58-59	7.458431365090588E-4
60-61	7.028137247873823E-5
62-63	3.1124607812012646E-4
64-65	0.0022403980369752885
66-67	0.002038159801883409
68-69	0.008528429403236277
70-71	0.008623094109023965
72-73	6.870362738227677E-4
74-75	0.0012837107830300147
76-77	0.0
78-79	0.0013253058810276353
80-81	0.0086804666579862
82-83	0.005731517641327306
84-85	1.1617941164852646E-4
86-87	0.010097568617353413
88-89	1.5203725474992352E-4
90-91	0.0021873284291852205
92-93	6.368352934808117E-4
94-95	0.014192534299532955
96-97	0.0010470490185607942
98-99	3.1985196046446177E-4
100-101	0.015540789200145486
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3.4859877E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.902010605328517
#Duplication Level	Percentage of deduplicated	Percentage of total
1	61.70944251976523	15.983986345958694
2	15.535300705326852	8.047910472526874
3	6.676340149190743	5.187919000473577
4	3.784369293332182	3.9209109428147904
5	2.4590550665992947	3.1847235207070876
6	1.6399680113937256	2.5487081294111875
7	1.18192731990246	2.143000578238872
8	0.8766978298813873	1.816658918980495
9	0.7348562433758135	1.7130828788380976
>10	4.672585840717768	22.798438423916256
>50	0.4192202808056251	7.515385540165082
>100	0.27117953506671677	13.516136062151324
>500	0.02557137034964664	4.527438904854413
>1k	0.012879609346797135	5.959567993141186
>5k	5.595922575817454E-4	0.9580333451166614
>10k+	4.663268813181211E-5	0.1780989427053296
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.302941172167647E-5	0.0	0.0	2.8686274481117647E-6	0.0
2	6.024117641034706E-5	5.7372548962235294E-6	0.0	2.8686274481117647E-6	0.0
3	1.2335098026880587E-4	5.7372548962235294E-6	0.0	2.8686274481117647E-6	1.1474509792447059E-5
4	2.094098037121588E-4	5.7372548962235294E-6	0.0	2.8686274481117647E-6	1.1474509792447059E-5
5	2.610450977781706E-4	5.7372548962235294E-6	0.0	8.605882344335294E-6	1.1474509792447059E-5
6	3.098117643960706E-4	5.7372548962235294E-6	0.0	8.605882344335294E-6	1.1474509792447059E-5
7	3.2702352908474115E-4	5.7372548962235294E-6	5.7372548962235294E-6	8.605882344335294E-6	1.4343137240558824E-5
8	3.585784310139706E-4	5.7372548962235294E-6	5.7372548962235294E-6	8.605882344335294E-6	1.4343137240558824E-5
9	3.815274505988647E-4	5.7372548962235294E-6	5.7372548962235294E-6	8.605882344335294E-6	2.5817647033005882E-5
10-11	4.4894019562949117E-4	5.7372548962235294E-6	5.7372548962235294E-6	8.605882344335294E-6	3.4423529377341177E-5
12-13	5.220901955563412E-4	8.605882344335294E-6	5.7372548962235294E-6	1.0040196068391176E-5	4.302941172167647E-5
14-15	5.966745092072471E-4	8.605882344335294E-6	5.7372548962235294E-6	1.4343137240558824E-5	5.1635294066011765E-5
16-17	6.56915685617594E-4	1.2908823516502941E-5	5.7372548962235294E-6	1.4343137240558824E-5	5.593823523817941E-5
18-19	7.257627443722765E-4	1.4343137240558824E-5	5.7372548962235294E-6	1.4343137240558824E-5	6.167549013440295E-5
20-21	8.189931364359089E-4	1.4343137240558824E-5	5.7372548962235294E-6	1.4343137240558824E-5	7.314999992685001E-5
22-23	9.452127441528263E-4	1.4343137240558824E-5	5.7372548962235294E-6	1.7211764688670588E-5	7.458431365090588E-5
24-25	0.0010943813714546383	1.5777450964614706E-5	5.7372548962235294E-6	1.7211764688670588E-5	7.888725482307354E-5
26-27	0.0012722362732375677	3.4423529377341177E-5	5.7372548962235294E-6	1.7211764688670588E-5	8.605882344335294E-5
28-29	0.0014902519592940619	3.4423529377341177E-5	8.605882344335294E-6	1.7211764688670588E-5	9.466470578768824E-5
30-31	0.0018201441158269146	3.4423529377341177E-5	1.1474509792447059E-5	1.7211764688670588E-5	1.0900784302824706E-4
32-33	0.002327891174142697	3.4423529377341177E-5	1.7211764688670588E-5	1.7211764688670588E-5	1.1187647047635882E-4
34-35	0.003006321565621129	3.4423529377341177E-5	1.7211764688670588E-5	1.7211764688670588E-5	1.233509802688059E-4
36-37	0.003942928427429621	3.729215682545294E-5	1.7211764688670588E-5	1.7211764688670588E-5	1.3195686261314115E-4
38-39	0.005259628426112921	3.729215682545294E-5	1.7211764688670588E-5	1.7211764688670588E-5	1.5203725474992352E-4
40-41	0.007005188228288929	3.729215682545294E-5	2.0080392136782353E-5	1.864607841272647E-5	1.7498627433481763E-4
42-43	0.009218334304507157	4.87666666179E-5	2.0080392136782353E-5	2.0080392136782353E-5	1.778549017829294E-4
44-45	0.011610769596232367	5.1635294066011765E-5	2.2949019584894118E-5	2.0080392136782353E-5	1.778549017829294E-4
46-47	0.01449087155413658	5.306960779006765E-5	2.2949019584894118E-5	2.0080392136782353E-5	1.778549017829294E-4
48-49	0.01815554311909936	5.593823523817941E-5	2.2949019584894118E-5	2.0080392136782353E-5	1.792892155069853E-4
50-51	0.022874435271243214	5.880686268629118E-5	2.2949019584894118E-5	2.0080392136782353E-5	1.8215784295509707E-4
52-53	0.028593044089054012	6.167549013440295E-5	2.2949019584894118E-5	2.0080392136782353E-5	1.8359215667915294E-4
54-55	0.034961397023862126	6.597843130657059E-5	2.7251960757061765E-5	2.2949019584894118E-5	1.8789509785132057E-4
56-57	0.04207129015400714	8.319019599524118E-5	2.8686274481117647E-5	2.5817647033005882E-5	1.9363235274754412E-4
58-59	0.05055238720434957	8.605882344335294E-5	3.4423529377341177E-5	2.5817647033005882E-5	2.022382350918794E-4
60-61	0.061131885232985766	9.179607833957647E-5	3.4423529377341177E-5	2.5817647033005882E-5	2.0654117626404706E-4
62-63	0.07462447443517944	9.609901951174412E-5	3.4423529377341177E-5	2.5817647033005882E-5	2.1227843116027056E-4
64-65	0.09044638912523989	9.75333332358E-5	3.4423529377341177E-5	2.5817647033005882E-5	2.2949019584894118E-4
66-67	0.10883429106763629	9.75333332358E-5	3.4423529377341177E-5	2.5817647033005882E-5	2.3666176446922058E-4
68-69	0.13023855477172222	9.75333332358E-5	3.729215682545294E-5	2.7251960757061765E-5	2.4957058798572354E-4
70-71	0.15605620180472812	9.75333332358E-5	3.729215682545294E-5	2.8686274481117647E-5	2.581764703300588E-4
72-73	0.18811167922365302	9.75333332358E-5	3.729215682545294E-5	2.8686274481117647E-5	2.710852938465618E-4
74-75	0.22825955467370124	1.0040196068391176E-4	3.729215682545294E-5	2.8686274481117647E-5	2.7251960757061766E-4
76-77	0.2761914507042007	1.1187647047635882E-4	3.729215682545294E-5	2.8686274481117647E-5	2.7251960757061766E-4
78-79	0.33394409280331083	1.1187647047635882E-4	3.729215682545294E-5	2.8686274481117647E-5	2.7395392129467354E-4
80-81	0.40258891332290125	1.1187647047635882E-4	3.729215682545294E-5	2.8686274481117647E-5	2.7825686246684116E-4
82-83	0.48599138774930273	1.1187647047635882E-4	3.729215682545294E-5	2.8686274481117647E-5	2.839941173630647E-4
84-85	0.5895201523516563	1.1474509792447059E-4	3.729215682545294E-5	2.8686274481117647E-5	2.839941173630647E-4
86-87	0.7168513532047173	1.1474509792447059E-4	4.0160784273564706E-5	2.8686274481117647E-5	2.839941173630647E-4
88-89	0.8668031157998636	1.2048235282069412E-4	4.0160784273564706E-5	2.8686274481117647E-5	2.911656859833441E-4
90	0.9946277205739997	1.2621960771691765E-4	4.0160784273564706E-5	2.8686274481117647E-5	2.954686271555118E-4
>>END_MODULE
