##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	SRR19654113_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24273431
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.624370160114573	32.0	32.0	32.0	32.0	32.0
2	31.611946494090596	32.0	32.0	32.0	32.0	32.0
3	35.72448522007458	37.0	37.0	37.0	32.0	37.0
4	36.336452477608134	37.0	37.0	37.0	37.0	37.0
5	36.51629376168536	37.0	37.0	37.0	37.0	37.0
6	40.08202503387346	41.0	41.0	41.0	37.0	41.0
7	40.112614446635085	41.0	41.0	41.0	37.0	41.0
8	40.211129650357215	41.0	41.0	41.0	37.0	41.0
9	40.226848359426405	41.0	41.0	41.0	37.0	41.0
10-11	40.28425165770756	41.0	41.0	41.0	41.0	41.0
12-13	40.26484916779997	41.0	41.0	41.0	41.0	41.0
14-15	40.20917461153308	41.0	41.0	41.0	41.0	41.0
16-17	40.22962246251879	41.0	41.0	41.0	41.0	41.0
18-19	40.218692054699645	41.0	41.0	41.0	41.0	41.0
20-21	40.19178578421814	41.0	41.0	41.0	41.0	41.0
22-23	40.17803389640302	41.0	41.0	41.0	39.0	41.0
24-25	40.15541434995325	41.0	41.0	41.0	37.0	41.0
26-27	40.089243481895906	41.0	41.0	41.0	37.0	41.0
28-29	40.05395257061105	41.0	41.0	41.0	37.0	41.0
30-31	40.018227130725776	41.0	41.0	41.0	37.0	41.0
32-33	40.00090003757606	41.0	41.0	41.0	37.0	41.0
34-35	39.967987838225255	41.0	41.0	41.0	37.0	41.0
36-37	39.84220685571809	41.0	41.0	41.0	37.0	41.0
38-39	39.85534024835632	41.0	41.0	41.0	37.0	41.0
40-41	39.839126079868976	41.0	41.0	41.0	37.0	41.0
42-43	39.79023278579777	41.0	41.0	41.0	37.0	41.0
44-45	39.75996127205915	41.0	41.0	41.0	37.0	41.0
46-47	39.722656801174914	41.0	41.0	41.0	37.0	41.0
48-49	39.6739170082713	41.0	41.0	41.0	37.0	41.0
50-51	39.574023260246975	41.0	41.0	41.0	37.0	41.0
52-53	39.52693566476037	41.0	41.0	41.0	37.0	41.0
54-55	39.438480081369626	41.0	41.0	41.0	37.0	41.0
56-57	39.37353440063747	41.0	41.0	41.0	37.0	41.0
58-59	39.28838238813458	41.0	41.0	41.0	37.0	41.0
60-61	39.219147861709374	41.0	41.0	41.0	37.0	41.0
62-63	39.09979448311201	41.0	41.0	41.0	37.0	41.0
64-65	38.99985037137931	41.0	41.0	41.0	37.0	41.0
66-67	38.86004238543781	41.0	41.0	41.0	32.0	41.0
68-69	38.70397534654248	41.0	41.0	41.0	32.0	41.0
70-71	38.619338362178794	41.0	41.0	41.0	32.0	41.0
72-73	38.475548162103664	41.0	37.0	41.0	32.0	41.0
74-75	38.30096285111075	41.0	37.0	41.0	32.0	41.0
76-77	36.572786826056856	39.0	34.5	41.0	27.0	41.0
78-79	37.330309753079405	41.0	37.0	41.0	29.5	41.0
80-81	38.184287544682086	41.0	37.0	41.0	32.0	41.0
82-83	38.25329322006436	41.0	37.0	41.0	32.0	41.0
84-85	38.25505714457919	41.0	37.0	41.0	32.0	41.0
86-87	38.212966947276634	41.0	37.0	41.0	32.0	41.0
88-89	38.09553952220434	41.0	37.0	41.0	32.0	41.0
90-91	38.00220753712155	41.0	37.0	41.0	32.0	41.0
92-93	37.97196296642201	41.0	37.0	41.0	32.0	41.0
94-95	37.946364154288695	41.0	37.0	41.0	32.0	41.0
96-97	37.92426118087715	41.0	37.0	41.0	32.0	41.0
98-99	37.89500641668663	41.0	37.0	41.0	32.0	41.0
100-101	37.00990377091726	41.0	37.0	41.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	10.0
17	9.0
18	56.0
19	183.0
20	620.0
21	2028.0
22	5130.0
23	11604.0
24	22627.0
25	39495.0
26	64458.0
27	95820.0
28	133081.0
29	175056.0
30	221344.0
31	270527.0
32	325947.0
33	396307.0
34	467719.0
35	570246.0
36	735882.0
37	1064743.0
38	1918031.0
39	5161095.0
40	1.2591413E7
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.10840131562112	11.329960066299446	5.2108392167573445	45.35079940132209
2	23.955257911417632	12.289202132158408	31.629929036401983	32.12561092002198
3	20.280818861518277	13.641834952280677	20.82853322130703	45.24881296489402
4	23.98992132591392	20.79275896349387	20.857158594514306	34.360161116077904
5	25.38064355220323	25.85406653060295	23.74760700289959	25.01768291429423
6	20.754247720480883	30.06494631929042	25.593856097228283	23.586949863000413
7	16.40359370704537	25.615604155836063	39.720285113381784	18.260517023736778
8	18.200845145808497	24.67481842531898	32.91251407724929	24.211822351623237
9	18.377558574228754	22.785522162070947	35.18668209698085	23.65023716671945
10-11	21.058121120166327	30.871869741034963	26.141397151478092	21.928611987320622
12-13	21.686209090095257	25.478307949131707	28.015681013532863	24.819801947240176
14-15	21.334137413101583	26.12921077933803	27.490122516147856	25.046529291412533
16-17	21.95954292576109	26.40435544526029	26.81687644404287	24.819225184935743
18-19	21.662226077557804	26.580278247438528	26.61876065233629	25.138735022667376
20-21	21.94190017884163	26.55023099124306	26.79308911871585	24.71477971119946
22-23	21.809096538515714	26.69086006012088	26.753642696823537	24.746400704539873
24-25	21.792947315291407	26.286581070131167	26.68220514186053	25.2382664727169
26-27	21.62779336798329	26.498460394824285	26.775032338856423	25.098713898336005
28-29	21.69524346622455	26.62285634350652	26.686580749539996	24.995319440728938
30-31	21.747786705554727	26.491040759750856	26.42575744648542	25.335415088208997
32-33	21.893044486896443	26.443713956600735	26.532372631249846	25.13086892525298
34-35	22.15905640632175	26.510891710576846	26.369844390245227	24.960207492856178
36-37	21.831205073563765	26.4119213307752	26.32816308497962	25.428710510681412
38-39	21.747325295711185	26.41093671512692	26.686713551125095	25.1550244380368
40-41	22.045795668523333	26.83481581157604	26.277327667440176	24.842060852460452
42-43	21.975848820053496	26.451524302435857	26.41856027687227	25.15406660063837
44-45	21.96098071179142	26.490237412255404	26.563041293997543	24.985740581955636
46-47	21.983289053780656	26.42467601716461	26.55515406948445	25.03688085957029
48-49	21.770406087215278	26.296836652387544	26.5091037192064	25.423653541190777
50-51	21.843123454611753	26.264562681723895	26.646125963816157	25.246187899848195
52-53	22.279349384106435	26.55014241703202	26.09716154259363	25.07334665626792
54-55	22.11428413230911	26.44106636593731	26.129806289024405	25.314843212729176
56-57	21.906042042429025	26.311478175458593	26.74688015880409	25.0355996233083
58-59	21.92237471497128	26.36402740098835	26.652744311259504	25.06085357278087
60-61	22.061324993570132	26.08735246368756	26.566120792730125	25.28520175001218
62-63	21.87370421593882	26.203561828568855	26.70402465971951	25.21870929577281
64-65	22.05996960215472	26.550130057839784	26.253567120363	25.1363332196425
66-67	22.01764760820174	26.417297579398646	26.20860231913651	25.35645249326311
68-69	22.01514693163896	26.50196010609295	26.556319953285552	24.926573008982537
70-71	22.2101564463631	26.85696348406618	26.272954573253365	24.659925496317353
72-73	22.260472100661527	26.389623631605346	26.149169307586384	25.200734960146743
74-75	22.161378228212705	26.344875386744697	26.486506428333822	25.007239956708776
76-77	22.046290036212845	26.550263949089025	26.4904125008121	24.913033513886027
78-79	22.00594057847761	26.42419535372079	26.328072314912788	25.24179175288881
80-81	22.118593370669355	26.422824198194313	26.494095540098968	24.964486891037367
82-83	22.35723289385831	26.66338557577625	26.12119811163078	24.858183418734665
84-85	22.255162444897056	26.43458808933933	26.12076142017171	25.189488045591908
86-87	22.181055359839927	26.37447720265979	26.399366968168664	25.045100469331622
88-89	22.385672162623653	26.363611982308715	26.37797955038802	24.872736304679616
90-91	22.340475889049223	26.21431638568112	26.35713303158503	25.088074693684632
92-93	22.193778351319665	26.21839737677644	26.49782327039364	25.09000100151026
94-95	22.453251523030872	26.32759766808998	26.30424874058439	24.914902068294758
96-97	22.45496320647872	26.25239299710041	26.141720550341645	25.15092324607922
98-99	22.330590595124356	26.49981372637432	26.31274087293222	24.8568548055691
100-101	22.413901850134	26.738234903833742	26.108439305510622	24.739423940521636
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	46.0
1	39.5
2	27.5
3	26.0
4	39.5
5	50.0
6	74.0
7	106.0
8	131.0
9	163.0
10	181.0
11	208.0
12	267.0
13	322.5
14	343.5
15	418.5
16	504.5
17	584.5
18	744.5
19	949.0
20	1208.5
21	1586.0
22	2291.0
23	3176.5
24	4476.0
25	6440.0
26	9254.5
27	13449.5
28	18564.5
29	25674.5
30	36324.0
31	50593.0
32	69767.5
33	95626.0
34	127626.0
35	172582.0
36	236746.0
37	317187.5
38	419897.5
39	563127.0
40	730909.0
41	902676.5
42	1076335.0
43	1238441.5
44	1383872.5
45	1493360.0
46	1558603.0
47	1556357.0
48	1489253.5
49	1402197.0
50	1305243.5
51	1184410.0
52	1065677.5
53	933854.5
54	798451.5
55	701314.0
56	605509.5
57	497728.0
58	399054.5
59	316009.0
60	249645.5
61	217674.5
62	191536.5
63	159143.0
64	131489.5
65	109866.5
66	96394.5
67	79466.5
68	57908.5
69	41615.5
70	31494.0
71	23772.5
72	18458.0
73	16094.5
74	13038.5
75	8557.0
76	5060.5
77	1773.0
78	258.5
79	82.0
80	20.0
81	6.5
82	4.5
83	1.5
84	0.5
85	0.5
86	1.0
87	0.5
88	0.0
89	1.0
90	1.0
91	0.0
92	0.0
93	0.0
94	0.5
95	1.0
96	0.5
97	0.0
98	0.5
99	0.5
100	2.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.024545355784272933
2	0.0
3	0.0023070492177228673
4	0.0
5	0.0
6	0.0
7	0.0
8	9.186999563432133E-4
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0022617321795175968
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	5.582235160740153E-4
26-27	0.0
28-29	6.447378617386228E-4
30-31	0.0
32-33	1.029932686483423E-5
34-35	7.003542268087277E-5
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	2.4718384475602152E-5
74-75	6.200194772630206E-4
76-77	0.0
78-79	0.001540779298979201
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0016561317598653442
88-89	4.9436768951204305E-5
90-91	0.0
92-93	3.707757671340323E-4
94-95	0.0015140010491306317
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2.4273431E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.228692704157833
#Duplication Level	Percentage of deduplicated	Percentage of total
1	62.679101346088665	18.320281922175912
2	15.404592915786907	9.005122251363645
3	6.6085647231229645	5.794791225230971
4	3.6983457318107336	4.3239124363531865
5	2.311274649105421	3.37777682368063
6	1.5975247108210366	2.8016135315932016
7	1.1385683690037394	2.329520548619914
8	0.8566200736369225	2.003030791723733
9	0.717396308238979	1.887170061655297
>10	4.393339669477993	24.068468144671826
>50	0.35687647603221234	7.19878321606275
>100	0.21474337700227517	11.803052276742433
>500	0.015650251210470718	3.1579553835137317
>1k	0.007298647093939722	3.6517378113793333
>5k	8.807277245869279E-5	0.1753108517424704
>10k+	1.467879540978213E-5	0.10147272349088783
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.9436768951204305E-5	0.0	0.0	0.0	0.0
2	6.179596118900538E-5	0.0	0.0	0.0	0.0
3	8.239461491867384E-5	0.0	0.0	0.0	0.0
4	1.441905761076792E-4	0.0	4.119730745933692E-6	0.0	0.0
5	1.7302869132921505E-4	0.0	4.119730745933692E-6	0.0	0.0
6	2.1834572953448566E-4	0.0	1.6478922983734767E-5	0.0	0.0
7	2.4718384475602153E-4	0.0	2.059865372966846E-5	4.119730745933692E-6	0.0
8	2.719022292316237E-4	0.0	2.059865372966846E-5	4.119730745933692E-6	0.0
9	2.966206137072258E-4	4.119730745933692E-6	2.059865372966846E-5	4.119730745933692E-6	4.119730745933692E-6
10-11	3.460573826584301E-4	4.119730745933692E-6	2.059865372966846E-5	8.239461491867384E-6	8.239461491867384E-6
12-13	3.9549415160963444E-4	1.2359192237801076E-5	2.059865372966846E-5	1.6478922983734767E-5	1.2359192237801076E-5
14-15	4.4081118981490505E-4	1.6478922983734767E-5	2.059865372966846E-5	1.6478922983734767E-5	1.2359192237801076E-5
16-17	4.8612822802017566E-4	2.059865372966846E-5	2.059865372966846E-5	1.6478922983734767E-5	1.6478922983734767E-5
18-19	5.60283381446982E-4	2.059865372966846E-5	2.059865372966846E-5	1.6478922983734767E-5	2.4718384475602152E-5
20-21	6.179596118900538E-4	2.059865372966846E-5	2.059865372966846E-5	2.059865372966846E-5	2.4718384475602152E-5
22-23	6.982943614357608E-4	2.059865372966846E-5	2.059865372966846E-5	2.059865372966846E-5	3.2957845967469534E-5
24-25	8.239461491867384E-4	2.2658519102635306E-5	2.059865372966846E-5	2.059865372966846E-5	3.2957845967469534E-5
26-27	9.248795524621138E-4	3.2957845967469534E-5	2.059865372966846E-5	2.059865372966846E-5	3.9137442086370077E-5
28-29	0.001067010263196826	3.707757671340323E-5	2.059865372966846E-5	2.059865372966846E-5	4.531703820527061E-5
30-31	0.001254458012136809	3.707757671340323E-5	2.4718384475602152E-5	2.059865372966846E-5	4.737690357823746E-5
32-33	0.0015428391643521676	4.325717283230376E-5	2.4718384475602152E-5	2.059865372966846E-5	4.9436768951204305E-5
34-35	0.0019074353353672993	4.531703820527061E-5	2.883811522153584E-5	3.2957845967469534E-5	5.767623044307169E-5
36-37	0.0023997431595063753	4.9436768951204305E-5	2.883811522153584E-5	3.2957845967469534E-5	6.179596118900538E-5
38-39	0.0031227559054177387	4.9436768951204305E-5	2.883811522153584E-5	3.2957845967469534E-5	7.20952880538396E-5
40-41	0.004237143072192803	4.9436768951204305E-5	2.883811522153584E-5	3.2957845967469534E-5	8.857421103757437E-5
42-43	0.005691408025507395	5.149663432417115E-5	2.883811522153584E-5	3.2957845967469534E-5	9.063407641054122E-5
44-45	0.007487610630734484	5.767623044307169E-5	2.883811522153584E-5	3.2957845967469534E-5	9.063407641054122E-5
46-47	0.009370327581626182	7.003542268087277E-5	2.883811522153584E-5	3.2957845967469534E-5	9.063407641054122E-5
48-49	0.011891602798137602	7.003542268087277E-5	3.2957845967469534E-5	3.2957845967469534E-5	9.887353790240861E-5
50-51	0.01558906114261309	7.003542268087277E-5	3.2957845967469534E-5	3.2957845967469534E-5	1.029932686483423E-4
52-53	0.02020521944343179	7.621501879977329E-5	3.2957845967469534E-5	3.2957845967469534E-5	1.029932686483423E-4
54-55	0.02554233062478889	7.827488417274014E-5	3.2957845967469534E-5	3.2957845967469534E-5	1.0917286476724283E-4
56-57	0.031410887072371434	1.0505313402130915E-4	3.2957845967469534E-5	3.2957845967469534E-5	1.1123273014020968E-4
58-59	0.03814046724585412	1.07112999394276E-4	4.531703820527061E-5	3.2957845967469534E-5	1.1123273014020968E-4
60-61	0.04715237825258407	1.07112999394276E-4	4.531703820527061E-5	3.2957845967469534E-5	1.1123273014020968E-4
62-63	0.0590048436086353	1.07112999394276E-4	4.531703820527061E-5	3.2957845967469534E-5	1.1123273014020968E-4
64-65	0.07380497631340209	1.07112999394276E-4	4.531703820527061E-5	3.2957845967469534E-5	1.1123273014020968E-4
66-67	0.09120465911885303	1.1123273014020968E-4	4.531703820527061E-5	3.2957845967469534E-5	1.1947219163207707E-4
68-69	0.11247276909473572	1.1123273014020968E-4	4.531703820527061E-5	3.2957845967469534E-5	1.2771165312394444E-4
70-71	0.1378606098165521	1.1329259551317653E-4	4.531703820527061E-5	3.2957845967469534E-5	1.3389124924284497E-4
72-73	0.16972260740560327	1.1535246088614337E-4	4.531703820527061E-5	3.707757671340323E-5	1.4007084536174554E-4
74-75	0.2094162131426744	1.1535246088614337E-4	4.531703820527061E-5	3.707757671340323E-5	1.4419057610767924E-4
76-77	0.25921757826489383	1.441905761076792E-4	4.531703820527061E-5	3.707757671340323E-5	1.483103068536129E-4
78-79	0.31872914875527897	1.4625044148064604E-4	4.531703820527061E-5	3.707757671340323E-5	1.483103068536129E-4
80-81	0.3902476745046879	1.483103068536129E-4	4.531703820527061E-5	3.707757671340323E-5	1.483103068536129E-4
82-83	0.4761172823075568	1.483103068536129E-4	4.531703820527061E-5	3.707757671340323E-5	1.5037017222657977E-4
84-85	0.5841242632737004	1.565497683454803E-4	4.531703820527061E-5	3.707757671340323E-5	1.524300375995466E-4
86-87	0.7163840991411556	1.6478922983734768E-4	4.737690357823746E-5	3.707757671340323E-5	1.524300375995466E-4
88-89	0.8731110159087111	1.7302869132921505E-4	4.9436768951204305E-5	3.707757671340323E-5	1.524300375995466E-4
90	1.0084112130666654	1.7302869132921505E-4	4.9436768951204305E-5	3.707757671340323E-5	1.524300375995466E-4
>>END_MODULE
