##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	SRR19654137_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	32808635
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.33646459232455	32.0	32.0	32.0	32.0	32.0
2	31.329890438904272	32.0	32.0	32.0	32.0	32.0
3	35.0607282198726	37.0	32.0	37.0	32.0	37.0
4	35.805246698011054	37.0	37.0	37.0	32.0	37.0
5	36.071193239218886	37.0	37.0	37.0	37.0	37.0
6	39.11687697461354	41.0	41.0	41.0	37.0	41.0
7	39.35874095341059	41.0	41.0	41.0	37.0	41.0
8	39.496980657683565	41.0	41.0	41.0	37.0	41.0
9	39.60856332486859	41.0	41.0	41.0	37.0	41.0
10-11	39.65158780607605	41.0	41.0	41.0	37.0	41.0
12-13	39.58696463598684	41.0	41.0	41.0	37.0	41.0
14-15	39.57171519022355	41.0	41.0	41.0	37.0	41.0
16-17	39.510925004956775	41.0	41.0	41.0	37.0	41.0
18-19	39.5319053200476	41.0	41.0	41.0	37.0	41.0
20-21	39.44802502450955	41.0	41.0	41.0	37.0	41.0
22-23	39.37037462850863	41.0	41.0	41.0	37.0	41.0
24-25	39.372914203836885	41.0	41.0	41.0	37.0	41.0
26-27	39.1934383585297	41.0	41.0	41.0	37.0	41.0
28-29	39.28004731071561	41.0	41.0	41.0	37.0	41.0
30-31	39.3680027376939	41.0	41.0	41.0	37.0	41.0
32-33	39.352778087232224	41.0	41.0	41.0	37.0	41.0
34-35	39.13992410839403	41.0	41.0	41.0	37.0	41.0
36-37	39.012550232583585	41.0	41.0	41.0	37.0	41.0
38-39	39.06621974672217	41.0	41.0	41.0	37.0	41.0
40-41	39.038928684475906	41.0	41.0	41.0	37.0	41.0
42-43	38.911970964351305	41.0	41.0	41.0	37.0	41.0
44-45	38.933133198013266	41.0	41.0	41.0	37.0	41.0
46-47	38.91357397221799	41.0	41.0	41.0	34.5	41.0
48-49	38.94854266262525	41.0	41.0	41.0	34.5	41.0
50-51	38.86006814974168	41.0	41.0	41.0	32.0	41.0
52-53	38.93889316029149	41.0	41.0	41.0	34.5	41.0
54-55	38.811593091270026	41.0	41.0	41.0	32.0	41.0
56-57	38.7958808862362	41.0	41.0	41.0	32.0	41.0
58-59	38.787317226090025	41.0	41.0	41.0	32.0	41.0
60-61	38.80455869316111	41.0	41.0	41.0	32.0	41.0
62-63	38.81912002739523	41.0	41.0	41.0	32.0	41.0
64-65	38.81528829224379	41.0	41.0	41.0	32.0	41.0
66-67	38.76234703150558	41.0	41.0	41.0	32.0	41.0
68-69	38.71540946461198	41.0	41.0	41.0	32.0	41.0
70-71	38.670769737905886	41.0	41.0	41.0	32.0	41.0
72-73	38.626175075555565	41.0	41.0	41.0	32.0	41.0
74-75	38.60527533376502	41.0	41.0	41.0	32.0	41.0
76-77	37.78003507308365	41.0	39.0	41.0	29.5	41.0
78-79	38.33644215920595	41.0	39.0	41.0	32.0	41.0
80-81	38.75432847175751	41.0	41.0	41.0	32.0	41.0
82-83	38.793045108399056	41.0	41.0	41.0	32.0	41.0
84-85	38.671296077998974	41.0	41.0	41.0	32.0	41.0
86-87	38.71219451830288	41.0	41.0	41.0	32.0	41.0
88-89	38.59319095414972	41.0	41.0	41.0	32.0	41.0
90-91	38.49704894763224	41.0	41.0	41.0	32.0	41.0
92-93	38.47971602597914	41.0	41.0	41.0	32.0	41.0
94-95	38.30494110772972	41.0	37.0	41.0	32.0	41.0
96-97	38.27066030025327	41.0	37.0	41.0	32.0	41.0
98-99	38.194429987715125	41.0	37.0	41.0	32.0	41.0
100-101	37.092879283152136	41.0	37.0	41.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	43.0
13	144.0
14	436.0
15	1992.0
16	9067.0
17	24695.0
18	42044.0
19	55324.0
20	66028.0
21	77112.0
22	90308.0
23	105822.0
24	122468.0
25	140695.0
26	160728.0
27	185157.0
28	212049.0
29	244986.0
30	285755.0
31	334755.0
32	396497.0
33	474190.0
34	585058.0
35	740668.0
36	966437.0
37	1351418.0
38	2256733.0
39	5632591.0
40	1.8245435E7
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	34.780816747966895	21.19341603299793	11.393451215394515	32.63231600364065
2	29.537960973159027	25.390399328476608	28.828655550143704	16.242984148220657
3	21.456761338026	27.697989873961582	28.75946275693204	22.08578603108038
4	25.061673648020705	31.315506677018025	23.053412578043982	20.569407096917292
5	27.70794002282052	32.763499117937826	21.54578385864325	17.982777000598407
6	22.8640188504421	37.02742334425938	21.547543852146706	18.561013953151814
7	22.155347066906835	22.022899327708544	35.82120920774537	20.000544397639253
8	23.436389763894017	24.916902709245107	26.60992256120784	25.036784965653037
9	23.77480451942071	23.61275915165563	29.058471697871084	23.553964631052573
10-11	25.28436875265478	30.169186319696124	23.189424574782354	21.357020352866744
12-13	25.70995294189941	25.12819022367838	26.007498254036687	23.154358580385516
14-15	24.86841833325296	26.813984930933188	26.108255321473408	22.209341414340443
16-17	25.68394728015792	26.350344281724215	25.62293278261587	22.34277565550199
18-19	25.232386962503984	26.847981315640713	25.76576919587703	22.153862525978273
20-21	25.449111862682443	26.834823961549603	25.51596746330288	22.200096712465076
22-23	25.4493118740618	26.638040533345247	25.65379398026956	22.258853612323392
24-25	24.972904045830244	26.720137743622384	26.031991195899202	22.27496701464817
26-27	25.19750788709355	26.896440367146983	25.710081520123456	22.19597022563601
28-29	25.401431559341475	26.635759952672743	25.63840315128141	22.324405336704377
30-31	24.95289739545642	26.64204184852422	26.032047573264787	22.37301318275457
32-33	25.126652998286563	26.860808466521295	25.801225277180954	22.211313258011188
34-35	25.361089967644208	26.66867548864752	25.619393424056064	22.350841119652205
36-37	25.019707341726853	26.670890022280446	26.037066613566083	22.27233602242661
38-39	25.22078049908657	26.830844747991627	25.777677807026066	22.170696945895738
40-41	25.448434151518363	26.523153513002118	25.678621420457958	22.34979091502156
42-43	24.925919319468726	26.69902553774042	26.077749967150194	22.29730517564066
44-45	25.109075234809435	26.760250206734842	25.903311663814005	22.22736289464172
46-47	25.43285584928099	26.530073350736238	25.7625795142519	22.27449128573087
48-49	24.987934483755254	26.588759668283164	26.080361146868835	22.342944701092755
50-51	25.224365756332713	26.68976778388655	25.778409093766868	22.307457366013868
52-53	25.446851694019585	26.565586763229227	25.73281172192105	22.254749820830135
54-55	24.965178091388886	26.624883069001005	26.119446992106038	22.290491847504068
56-57	25.05765290468489	26.79894880291594	25.915082394933258	22.22831589746591
58-59	25.41389244516303	26.44139293584097	25.82469481569454	22.32001980330147
60-61	25.07383567280856	26.569288563377746	26.045867630859536	22.311008132954154
62-63	25.160946514372196	26.65486049031044	25.98826797043077	22.195925024886602
64-65	25.33665947302924	26.492703897145365	25.92963047627594	22.241006153549453
66-67	25.009415036863203	26.61109303037932	26.197642221771293	22.18184971098618
68-69	25.188836714260148	26.78261173881438	25.9201616758918	22.10838987103367
70-71	25.492060118764904	26.569303210427197	25.7184988829403	22.220137787867596
72-73	25.043594056583224	26.64820618233784	26.18278074162462	22.125419019454316
74-75	25.05109434135276	26.81330345325927	26.01200084477441	22.123601360613566
76-77	25.43141869093901	26.526352605027164	25.83681395703879	22.205414746995036
78-79	24.998901824781594	26.69712272605712	26.165900088033872	22.138075361127413
80-81	25.261704008975688	26.736460803148436	26.002359744981735	21.99947544289414
82-83	25.486673582629688	26.437848129390307	25.93554260686286	22.139935681117144
84-85	25.170803274045316	26.64633809334237	26.186893557493928	21.99596507511838
86-87	25.27292594138458	26.839904921182416	25.92886627119226	21.958302866240743
88-89	25.492343035905453	26.618832077589893	25.832231208822176	22.056593677682486
90-91	25.07836949986232	26.722214795037434	26.182435758560125	22.01697994654012
92-93	25.3384642941691	26.820053393663006	25.922262770006753	21.919219542161137
94-95	25.658240998179867	26.646489546966635	25.780221250767028	21.915048204086474
96-97	25.296610875981557	26.6449410554857	26.146779203314736	21.911668865218008
98-99	25.472385900798063	26.903932963899212	25.83972078086447	21.783960354438257
100-101	25.794511025345813	26.62409389449279	25.766487996103905	21.814907084057488
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	110.0
1	64.5
2	19.0
3	24.5
4	38.5
5	51.0
6	64.5
7	78.0
8	101.0
9	144.5
10	174.5
11	209.0
12	257.0
13	317.5
14	397.5
15	472.5
16	596.5
17	755.5
18	911.0
19	1126.5
20	1452.0
21	1903.5
22	2488.0
23	3161.0
24	4169.0
25	5582.5
26	7437.5
27	10105.0
28	14061.5
29	19561.0
30	27189.5
31	37785.5
32	53761.5
33	77745.5
34	114500.5
35	172147.0
36	255542.5
37	372801.0
38	543132.5
39	767742.5
40	1019072.0
41	1288924.0
42	1575272.0
43	1827111.0
44	2025096.5
45	2152880.5
46	2193613.0
47	2176695.0
48	2100512.5
49	1968655.5
50	1788099.0
51	1600406.0
52	1418274.0
53	1237495.5
54	1072607.5
55	911488.5
56	749014.5
57	594727.5
58	471118.0
59	378626.5
60	314252.5
61	268842.0
62	231856.5
63	194199.0
64	158162.0
65	130874.5
66	111448.5
67	93280.0
68	71241.0
69	52389.0
70	40663.5
71	31644.5
72	22671.5
73	15123.0
74	8835.5
75	4850.0
76	2803.5
77	1573.0
78	981.5
79	659.5
80	503.0
81	392.0
82	305.0
83	273.0
84	238.5
85	201.5
86	174.5
87	139.0
88	118.0
89	96.5
90	78.0
91	66.0
92	65.5
93	68.5
94	63.5
95	58.0
96	60.0
97	72.5
98	88.0
99	151.5
100	679.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.007485834140920524
2	0.006199587395208609
3	0.0016550520922312068
4	1.584948596611837E-4
5	0.014706494189715603
6	0.0014996052106404304
7	0.005282145996015988
8	0.009488355733178171
9	0.0035234626493909306
10-11	0.006705551754896233
12-13	0.004666454425793698
14-15	0.009610274855994467
16-17	1.1887114474588778E-4
18-19	0.013103257724681324
20-21	0.0064281857504891625
22-23	0.008627301928288087
24-25	0.01195416999213774
26-27	0.010056803643309147
28-29	0.0066171603908544195
30-31	0.01114035984733897
32-33	0.005402541129797079
34-35	0.005119079169249193
36-37	0.009044874923933898
38-39	0.010620679586334511
40-41	0.009005251209018602
42-43	0.012281827634706534
44-45	0.008301168274754496
46-47	0.004468335851217218
48-49	0.010261018174026443
50-51	0.01048352057316618
52-53	0.007548317691363874
54-55	0.01066030330124981
56-57	0.012588149430782476
58-59	0.00604718849168824
60-61	0.00248257813834681
62-63	0.0132175569023216
64-65	0.004981920156080861
66-67	0.006315410561884089
68-69	0.01593635334112498
70-71	0.006013660732913759
72-73	0.00604718849168824
74-75	0.014054226882648424
76-77	0.00650590919128455
78-79	0.01285027554483751
80-81	1.5239890352036895E-5
82-83	0.007333435237400154
84-85	0.016904086378479326
86-87	0.0021229167260387394
88-89	0.010465232704743737
90-91	0.0037459650485306686
92-93	0.012170576435136663
94-95	0.010134527084104535
96-97	0.0043464167284009226
98-99	0.009591986987572021
100-101	0.01058715182756003
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3.2808635E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.03834553581139
#Duplication Level	Percentage of deduplicated	Percentage of total
1	62.0708266256588	19.88646591126298
2	15.09422241491306	9.67187826646748
3	6.96798168538847	6.697278147710437
4	4.013532211873247	5.143477272924177
5	2.541246860191072	4.070867249929867
6	1.7870109473580416	3.435172452464076
7	1.218040365370261	2.731679867160886
8	0.9335679885105916	2.3927979037659783
9	0.7262536405670059	2.0941168574814033
>10	4.199792060157176	24.598320373316707
>50	0.28115766299222444	6.210316353520259
>100	0.1525123960579922	9.15084156407234
>500	0.010614261532120252	2.292527535457715
>1k	0.00321100760524786	1.569909724393729
>5k	2.9871824871434445E-5	0.05435052007205348
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.6575736844888546E-5	0.0	2.438382456325903E-5	1.2191912281629516E-5	0.0
2	4.5719671056110684E-5	9.143934211222136E-6	3.3527758774481166E-5	2.743180263366641E-5	0.0
3	7.924742983059185E-5	1.2191912281629516E-5	3.3527758774481166E-5	2.743180263366641E-5	0.0
4	1.3715901316833204E-4	1.2191912281629516E-5	4.2671692985703305E-5	3.047978070407379E-5	0.0
5	1.5544688159077632E-4	1.5239890352036895E-5	4.2671692985703305E-5	3.047978070407379E-5	3.047978070407379E-6
6	1.9202261843566487E-4	1.5239890352036895E-5	4.5719671056110684E-5	3.047978070407379E-5	3.047978070407379E-6
7	2.164064429989239E-4	1.8287868422444273E-5	4.876764912651806E-5	3.047978070407379E-5	3.047978070407379E-6
8	2.4993420177340505E-4	1.8287868422444273E-5	5.181562719692544E-5	3.047978070407379E-5	6.095956140814758E-6
9	2.6822207019584933E-4	2.438382456325903E-5	5.79115833377402E-5	3.047978070407379E-5	6.095956140814758E-6
10-11	3.596614123080707E-4	2.438382456325903E-5	7.315147368977709E-5	3.5051747809684856E-5	7.619945176018447E-6
12-13	4.495767653850884E-4	2.438382456325903E-5	9.905928728823982E-5	3.6575736844888546E-5	1.0667923246425826E-5
14-15	5.638759430253651E-4	2.438382456325903E-5	1.1429917764027671E-4	3.8099725880092235E-5	1.2191912281629516E-5
16-17	7.16274846545734E-4	2.743180263366641E-5	1.7830671711883167E-4	3.9623714915295925E-5	1.8287868422444273E-5
18-19	9.1286943208701E-4	2.743180263366641E-5	2.2250239913973867E-4	4.7243660091314374E-5	1.8287868422444273E-5
20-21	0.0010728882807833973	2.743180263366641E-5	2.6517409212544195E-4	5.486360526733282E-5	1.8287868422444273E-5
22-23	0.0013289184386976172	3.3527758774481166E-5	3.017498289703305E-4	5.79115833377402E-5	1.8287868422444273E-5
24-25	0.0017038197413577249	4.2671692985703305E-5	3.2613365353358954E-4	5.79115833377402E-5	2.1335846492851652E-5
26-27	0.0021777803313060722	5.333961623212913E-5	3.33753598709608E-4	5.79115833377402E-5	2.1335846492851652E-5
28-29	0.002804139824774789	5.943557237294389E-5	3.627093903784781E-4	6.400753947855495E-5	2.438382456325903E-5
30-31	0.003607282046327133	6.400753947855495E-5	3.7032933555449654E-4	6.400753947855495E-5	2.743180263366641E-5
32-33	0.004526247434554958	6.705551754896233E-5	3.733773136249039E-4	6.400753947855495E-5	2.743180263366641E-5
34-35	0.005553416044282245	6.705551754896233E-5	4.2976490792744044E-4	6.400753947855495E-5	2.743180263366641E-5
36-37	0.006862522625522213	6.705551754896233E-5	4.6938862284273637E-4	6.400753947855495E-5	2.743180263366641E-5
38-39	0.008692833456801845	6.857950658416602E-5	4.968204254764027E-4	6.705551754896233E-5	2.743180263366641E-5
40-41	0.011013868757417064	7.010349561936971E-5	5.196802610044581E-4	6.705551754896233E-5	3.047978070407379E-5
42-43	0.013982599397993851	7.010349561936971E-5	5.699718991661798E-4	6.705551754896233E-5	3.200376973927748E-5
44-45	0.017225648064907305	7.924742983059185E-5	5.943557237294389E-4	7.010349561936971E-5	3.3527758774481166E-5
46-47	0.020811594264741586	7.924742983059185E-5	6.629352303136049E-4	7.315147368977709E-5	3.3527758774481166E-5
48-49	0.024912648758474713	8.077141886579554E-5	6.766511316304381E-4	7.619945176018447E-5	3.3527758774481166E-5
50-51	0.030901925666825213	9.296333114742506E-5	6.842710768064566E-4	7.619945176018447E-5	3.5051747809684856E-5
52-53	0.03789551134937494	9.905928728823982E-5	7.101788904049193E-4	7.619945176018447E-5	3.8099725880092235E-5
54-55	0.04533562581923936	1.005832763234435E-4	7.36086704003382E-4	7.924742983059185E-5	4.2671692985703305E-5
56-57	0.05372670944707087	1.005832763234435E-4	7.665664847074558E-4	7.924742983059185E-5	4.2671692985703305E-5
58-59	0.06366006997852852	1.005832763234435E-4	7.818063750594927E-4	7.924742983059185E-5	4.2671692985703305E-5
60-61	0.07589465395314374	1.005832763234435E-4	8.351459912916218E-4	7.924742983059185E-5	4.2671692985703305E-5
62-63	0.09104920091920923	1.005832763234435E-4	9.342052785798617E-4	7.924742983059185E-5	4.5719671056110684E-5
64-65	0.10879148126705057	1.021072653586472E-4	9.966888290232128E-4	7.924742983059185E-5	5.181562719692544E-5
66-67	0.12955583187170086	1.1887114474588777E-4	0.0010104047303400462	7.924742983059185E-5	5.638759430253651E-5
68-69	0.15385278905995325	1.4020699123873942E-4	0.001030216587797694	7.924742983059185E-5	5.943557237294389E-5
70-71	0.18274762116741522	1.4020699123873942E-4	0.0010332645658681014	7.924742983059185E-5	7.16274846545734E-5
72-73	0.21819560612625305	1.447789583443505E-4	0.0010683163136777861	7.924742983059185E-5	7.924742983059185E-5
74-75	0.2616704413335087	1.4782693641475787E-4	0.00106984030271299	8.077141886579554E-5	7.924742983059185E-5
76-77	0.31342663295806117	1.4935092544996156E-4	0.0010774602478890085	8.229540790099923E-5	7.924742983059185E-5
78-79	0.3752396282259228	1.5544688159077632E-4	0.0010835562040298233	8.229540790099923E-5	8.229540790099923E-5
80-81	0.4487858150758177	1.6154283773159108E-4	0.0011003200834170637	8.839136404181399E-5	8.229540790099923E-5
82-83	0.5375200766505526	1.6611480483720215E-4	0.0011247039079803228	8.839136404181399E-5	8.229540790099923E-5
84-85	0.6455160356412268	1.737347500132206E-4	0.001138419809297156	8.839136404181399E-5	8.229540790099923E-5
86-87	0.7776672208398795	1.7983070615403536E-4	0.001147563743508378	8.839136404181399E-5	8.229540790099923E-5
88-89	0.9366177532225891	1.8287868422444274E-4	0.0011506117215787856	8.839136404181399E-5	9.448732018262875E-5
90	1.0707181203972673	2.01166552646887E-4	0.0011521357106139892	8.839136404181399E-5	1.0363125439385089E-4
>>END_MODULE
