##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	SRR19654136_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	30386663
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.394181058973142	32.0	32.0	32.0	32.0	32.0
2	31.402983473374487	32.0	32.0	32.0	32.0	32.0
3	34.83064761010447	37.0	32.0	37.0	32.0	37.0
4	35.67257997365489	37.0	37.0	37.0	32.0	37.0
5	36.08447373770525	37.0	37.0	37.0	37.0	37.0
6	39.022326275181975	41.0	41.0	41.0	37.0	41.0
7	39.37821754234744	41.0	41.0	41.0	37.0	41.0
8	39.54157026061072	41.0	41.0	41.0	37.0	41.0
9	39.73124811368724	41.0	41.0	41.0	37.0	41.0
10-11	39.68841433822463	41.0	41.0	41.0	37.0	41.0
12-13	39.638578181487055	41.0	41.0	41.0	37.0	41.0
14-15	39.61551819625603	41.0	41.0	41.0	37.0	41.0
16-17	39.56977617121038	41.0	41.0	41.0	37.0	41.0
18-19	39.62580177033588	41.0	41.0	41.0	37.0	41.0
20-21	39.493686671024065	41.0	41.0	41.0	37.0	41.0
22-23	39.416295316797374	41.0	41.0	41.0	37.0	41.0
24-25	39.420811969382754	41.0	41.0	41.0	37.0	41.0
26-27	39.173358094635134	41.0	41.0	41.0	37.0	41.0
28-29	39.347201467959806	41.0	41.0	41.0	37.0	41.0
30-31	39.434952679733215	41.0	41.0	41.0	37.0	41.0
32-33	39.423746595669286	41.0	41.0	41.0	37.0	41.0
34-35	39.17474668080533	41.0	41.0	41.0	37.0	41.0
36-37	39.04805883094172	41.0	41.0	41.0	37.0	41.0
38-39	39.11168134849161	41.0	41.0	41.0	37.0	41.0
40-41	39.09354650097643	41.0	41.0	41.0	37.0	41.0
42-43	38.98065437458533	41.0	41.0	41.0	37.0	41.0
44-45	39.0055792240168	41.0	41.0	41.0	37.0	41.0
46-47	38.98870201706585	41.0	41.0	41.0	37.0	41.0
48-49	39.02802699987162	41.0	41.0	41.0	37.0	41.0
50-51	38.94358189314832	41.0	41.0	41.0	37.0	41.0
52-53	39.03122590328527	41.0	41.0	41.0	37.0	41.0
54-55	38.91090227643622	41.0	41.0	41.0	32.0	41.0
56-57	38.90072121443542	41.0	41.0	41.0	34.5	41.0
58-59	38.895752982155365	41.0	41.0	41.0	32.0	41.0
60-61	38.917208957758874	41.0	41.0	41.0	37.0	41.0
62-63	38.93525574032265	41.0	41.0	41.0	37.0	41.0
64-65	38.93420417701016	41.0	41.0	41.0	37.0	41.0
66-67	38.88607088906077	41.0	41.0	41.0	34.5	41.0
68-69	38.84303747009008	41.0	41.0	41.0	32.0	41.0
70-71	38.799743295273984	41.0	41.0	41.0	32.0	41.0
72-73	38.7598775489102	41.0	41.0	41.0	32.0	41.0
74-75	38.73980059936163	41.0	41.0	41.0	32.0	41.0
76-77	37.889340728200395	41.0	39.0	41.0	32.0	41.0
78-79	38.45468770295705	41.0	39.0	41.0	32.0	41.0
80-81	38.88031132276683	41.0	41.0	41.0	32.0	41.0
82-83	38.922195487540044	41.0	41.0	41.0	34.5	41.0
84-85	38.80090650296151	41.0	41.0	41.0	32.0	41.0
86-87	38.842621695577435	41.0	41.0	41.0	32.0	41.0
88-89	38.72458005671765	41.0	41.0	41.0	32.0	41.0
90-91	38.63143978659322	41.0	41.0	41.0	32.0	41.0
92-93	38.6167603201444	41.0	41.0	41.0	32.0	41.0
94-95	38.44230654744813	41.0	39.0	41.0	32.0	41.0
96-97	38.40796973988226	41.0	37.0	41.0	32.0	41.0
98-99	38.33550100581956	41.0	37.0	41.0	32.0	41.0
100-101	37.22505580820112	41.0	37.0	41.0	29.5	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	31.0
13	100.0
14	398.0
15	1520.0
16	6399.0
17	16632.0
18	28050.0
19	38175.0
20	47272.0
21	57101.0
22	68847.0
23	81947.0
24	97224.0
25	114489.0
26	133404.0
27	156928.0
28	182369.0
29	214932.0
30	251642.0
31	297110.0
32	355294.0
33	430690.0
34	535438.0
35	685674.0
36	898348.0
37	1277558.0
38	2176098.0
39	5648168.0
40	1.6584824E7
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	35.333635045345495	21.29176360105782	10.894182053984771	32.48041929961191
2	29.34930169097617	25.157798694917982	29.479001592805233	16.013898021300616
3	20.95071869867114	27.53458563550457	29.35146153661353	22.163234129210757
4	24.924309611411523	31.338149203108117	23.28257381974612	20.454967365734237
5	27.588784287554365	33.049804698364184	21.527965981698383	17.833445032383068
6	22.590033022902777	37.179281873630465	21.674794702750432	18.555890400716326
7	21.96362082011851	21.921962205651514	36.2032086019082	19.911208372321777
8	23.023653678345166	24.837594829943697	27.043900566391926	25.09485092531921
9	23.50450636616349	23.516818131659804	29.44678113482094	23.531894367355765
10-11	25.045024441322393	30.290831792202155	23.38775770942972	21.276386057045727
12-13	25.57046997535972	25.124703762126376	26.2255781677994	23.0792480947145
14-15	24.608673540737225	26.823335263209184	26.37904692613102	22.188944269922565
16-17	25.520090716236904	26.36339932750669	25.822909831833236	22.293600124423175
18-19	25.033900978336156	26.85866557060086	25.997631736897866	22.10980171416512
20-21	25.2203587053113	26.842854896893215	25.780238207677343	22.15654819011815
22-23	25.21411572220537	26.65841802119495	25.901580005396596	22.225886251203082
24-25	24.763365230114832	26.71674530095423	26.319902670413793	22.19998679851715
26-27	24.932770339887004	26.91726102084421	25.979649704400792	22.17031893486799
28-29	25.16301542921408	26.65075733342136	25.90673251982456	22.27949471754
30-31	24.744224696988084	26.6577238988993	26.314305620956258	22.283745783156363
32-33	24.84822605844359	26.903847794332147	26.09100407201029	22.156922075213973
34-35	25.14443647104796	26.693154235296106	25.87149581003948	22.290913483616453
36-37	24.784775685304304	26.68857035986159	26.322728601935935	22.203925352898167
38-39	24.92838559976516	26.881038694197922	26.064083819247298	22.12649188678962
40-41	25.209354060490213	26.56320542661536	25.95626505763773	22.271175455256703
42-43	24.69988683693941	26.719188541007505	26.365235538927966	22.215689083125117
44-45	24.856027317796077	26.790690652703642	26.183043234433796	22.17023879506649
46-47	25.173027366906055	26.563734638148727	26.029008868089132	22.234229126856082
48-49	24.74983290268281	26.59795654824666	26.391719556132653	22.260490992937875
50-51	24.95948279902389	26.736817271751622	26.064693500975793	22.239006428248693
52-53	25.19915589476074	26.61880553493914	25.99730657509588	22.184731995204242
54-55	24.722689021144422	26.670161672575084	26.390975900668767	22.216173405611727
56-57	24.81515673088891	26.8385565936957	26.197658967071046	22.14862770834435
58-59	25.160555680727846	26.4983501940741	26.08346328323655	22.2576308419615
60-61	24.85130361896999	26.5952829256954	26.33140898754031	22.222004467794303
62-63	24.90409709809442	26.699013739892102	26.25557503347398	22.141314128539495
64-65	25.109600573691125	26.544399395339635	26.17661584667549	22.16938418429375
66-67	24.786879960669673	26.66874677088668	26.431946635766025	22.112426632677625
68-69	24.939334180254523	26.835182293195388	26.18692601657409	22.03855750997599
70-71	25.289722818877756	26.618586460365094	25.972143166392105	22.11954755436504
72-73	24.833916582618357	26.674612852973077	26.427224409015075	22.064246155393484
74-75	24.816580179498303	26.861320038157682	26.258139224192313	22.063960558151706
76-77	25.217280049604017	26.591109289283878	26.045287363168917	22.146323297943187
78-79	24.792310291975166	26.728050846498114	26.424419660947862	22.055219200578858
80-81	25.02863510125513	26.79322210403073	26.250956135122074	21.92718665959207
82-83	25.294650980295618	26.505225275203486	26.146034144089263	22.054089600411633
84-85	24.958615667179792	26.680432671152737	26.43569053654585	21.92526112512162
86-87	25.040843218268378	26.900108294897475	26.14596522482064	21.913083262013508
88-89	25.290760988292384	26.675297471062958	26.03688508707093	21.99705645357373
90-91	24.88255950162534	26.769366112154803	26.388758504119735	21.95931588210012
92-93	25.135164605785203	26.872709612106842	26.135965910358816	21.85615987174914
94-95	25.478304162676274	26.68045457474687	25.97505426156255	21.866187001014303
96-97	25.089675307139725	26.69876506630316	26.33917195956053	21.87238766699658
98-99	25.29791848143995	26.969628910945797	26.016956245432795	21.715496362181458
100-101	25.633852371298786	26.68045322692893	25.926067328419368	21.759627073352917
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	70.0
1	44.5
2	18.5
3	19.0
4	33.5
5	54.5
6	67.0
7	75.5
8	84.0
9	122.5
10	163.0
11	195.5
12	247.5
13	310.5
14	377.0
15	440.5
16	529.5
17	671.5
18	849.5
19	1073.0
20	1347.5
21	1705.5
22	2206.0
23	2895.5
24	3931.5
25	5329.5
26	7193.5
27	9833.5
28	13728.0
29	19227.0
30	26748.5
31	37802.5
32	54695.0
33	79593.5
34	118375.5
35	179747.5
36	268490.5
37	391770.5
38	564620.0
39	786488.0
40	1026393.0
41	1270240.5
42	1522311.0
43	1738454.0
44	1900868.0
45	2000828.5
46	2021887.0
47	1991535.5
48	1911083.0
49	1778687.0
50	1606475.0
51	1436209.0
52	1270676.5
53	1102636.5
54	951549.5
55	808217.5
56	663746.5
57	527835.0
58	417970.5
59	334413.5
60	277763.5
61	237654.5
62	201930.0
63	167368.0
64	137549.5
65	113186.0
66	95223.5
67	79187.0
68	59986.5
69	44232.5
70	33856.5
71	25544.0
72	17941.0
73	11822.0
74	6946.5
75	3924.5
76	2287.0
77	1329.5
78	879.5
79	626.5
80	486.0
81	411.0
82	338.5
83	274.5
84	240.0
85	192.5
86	156.5
87	146.0
88	130.5
89	103.0
90	91.0
91	88.0
92	71.0
93	52.5
94	51.0
95	52.0
96	49.5
97	58.0
98	67.0
99	133.5
100	586.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.007503291822468299
2	0.006272488690186218
3	0.0016224223107354696
4	1.4480036850377418E-4
5	0.01457547345689127
6	0.0015763494662115415
7	0.005235849688397834
8	0.00964897001029695
9	0.0036002637077983853
10-11	0.006762835392619453
12-13	0.004781703078090543
14-15	0.009925407077440522
16-17	1.3492761610578956E-4
18-19	0.013357833994473168
20-21	0.0065818349319897345
22-23	0.008834467937463222
24-25	0.012217531092505946
26-27	0.010280826163767966
28-29	0.007215336544193747
30-31	0.011360247092614283
32-33	0.005499123085677423
34-35	0.005237495147130832
36-37	0.009537078816453125
38-39	0.010805727499594148
40-41	0.009176723353926688
42-43	0.013022160412941691
44-45	0.0082717210507781
46-47	0.004503620552213976
48-49	0.010435499284669726
50-51	0.010764591031269212
52-53	0.007569110171788195
54-55	0.010891291353710014
56-57	0.013043551376470657
58-59	0.006177052083672366
60-61	0.0024747699344281403
62-63	0.013525670785238905
64-65	0.005500768544410421
66-67	0.006474880114344902
68-69	0.0159658860862741
70-71	0.0066410714463776424
72-73	0.006109588275619472
74-75	0.014197017948301858
76-77	0.006673980621037591
78-79	0.013423652343793065
80-81	1.9745504795969207E-5
82-83	0.007511519116133286
84-85	0.017436926193573805
86-87	0.0021440327290956564
88-89	0.010680672635886342
90-91	0.0038487279764809978
92-93	0.012206012881374963
94-95	0.01078433653606518
96-97	0.004434511285428084
98-99	0.00964897001029695
100-101	0.010557263230911535
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3.0386663E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.328609687847226
#Duplication Level	Percentage of deduplicated	Percentage of total
1	62.792828291780424	21.55590493624541
2	15.134400960823886	10.390858868870064
3	6.964948048030188	7.172909491108852
4	3.9268761034536532	5.392167881919795
5	2.431810572989072	4.174033799746098
6	1.6817589582894545	3.463946812089554
7	1.228496921571694	2.952081392634959
8	0.9075602989735168	2.4924226617318235
9	0.6937096842229354	2.1432680087732012
>10	3.8686131031083146	24.011910994541815
>50	0.23961742786500076	5.67057254273836
>100	0.1192253468322927	7.534531582363173
>500	0.007920968891893988	1.8328111874217383
>1k	0.00221324375719085	1.1767989636091758
>5k	2.0069410709275204E-5	0.03578087620614935
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.6200092125943544E-5	0.0	6.581834931989735E-6	3.2909174659948676E-6	0.0
2	4.278192705793328E-5	1.6454587329974337E-5	9.872752397984602E-6	6.581834931989735E-6	0.0
3	9.543660651385116E-5	1.6454587329974337E-5	9.872752397984602E-6	9.872752397984602E-6	0.0
4	1.5467312090175877E-4	1.6454587329974337E-5	1.6454587329974337E-5	9.872752397984602E-6	3.2909174659948676E-6
5	1.6454587329974338E-4	2.632733972795894E-5	1.9745504795969204E-5	9.872752397984602E-6	3.2909174659948676E-6
6	2.4023697501762532E-4	2.632733972795894E-5	2.3036422261964074E-5	1.316366986397947E-5	3.2909174659948676E-6
7	2.6656431474558426E-4	2.9618257193953807E-5	2.3036422261964074E-5	1.316366986397947E-5	3.2909174659948676E-6
8	2.863098195415535E-4	3.2909174659948674E-5	4.607284452392815E-5	1.316366986397947E-5	9.872752397984602E-6
9	3.126371592695124E-4	3.2909174659948674E-5	5.594559692191275E-5	1.316366986397947E-5	1.316366986397947E-5
10-11	3.932646371863867E-4	4.607284452392815E-5	8.062747791687426E-5	1.6454587329974337E-5	1.316366986397947E-5
12-13	4.557920690402891E-4	4.607284452392815E-5	9.872752397984604E-5	1.810004606297177E-5	1.6454587329974337E-5
14-15	5.380650056901609E-4	5.265467945591788E-5	1.1847302877581524E-4	2.3036422261964074E-5	1.9745504795969204E-5
16-17	6.104651899420479E-4	5.430013818891531E-5	2.040368828916818E-4	2.961825719395381E-5	2.139096352896664E-5
18-19	7.338745949168554E-4	5.594559692191275E-5	2.4517335121661764E-4	4.278192705793328E-5	2.3036422261964074E-5
20-21	8.671567522896477E-4	6.417289058689992E-5	2.7643706714356885E-4	4.936376198992302E-5	2.3036422261964074E-5
22-23	0.0010876482225113037	6.746380805289478E-5	3.0605532433752267E-4	5.759105565491018E-5	2.3036422261964074E-5
24-25	0.0013871217119168367	7.569110171788195E-5	3.1099170053651496E-4	6.581834931989735E-5	2.3036422261964074E-5
26-27	0.001798486395166195	7.898201918387681E-5	3.1428261800250983E-4	6.581834931989735E-5	2.632733972795894E-5
28-29	0.002275669427735451	8.227293664987169E-5	3.3896449899747136E-4	7.075472551888966E-5	2.7972798460956372E-5
30-31	0.002940434755866414	9.708206524684859E-5	3.455463339294611E-4	7.240018425188709E-5	3.949100959193841E-5
32-33	0.0037516459112341494	1.069548176448332E-4	3.455463339294611E-4	7.240018425188709E-5	4.936376198992301E-5
34-35	0.004681330095377699	1.0860027637783063E-4	3.8832826098739436E-4	7.240018425188709E-5	5.265467945591788E-5
36-37	0.005877578594266833	1.0860027637783063E-4	4.2617381184633534E-4	7.240018425188709E-5	5.9236514387907614E-5
38-39	0.007427600720750416	1.118911938438255E-4	4.459193166423045E-4	7.240018425188709E-5	5.9236514387907614E-5
40-41	0.009410378494012325	1.118911938438255E-4	4.887012437002379E-4	7.240018425188709E-5	5.9236514387907614E-5
42-43	0.01197729411748832	1.1518211130982037E-4	5.512286755541404E-4	7.240018425188709E-5	6.746380805289478E-5
44-45	0.014860137817699825	1.267003224408024E-4	5.627468866851224E-4	7.240018425188709E-5	6.910926678589222E-5
46-47	0.0180819460169088	1.3492761610578956E-4	6.055288137430556E-4	7.240018425188709E-5	7.569110171788195E-5
48-49	0.022198883766868377	1.464458272367716E-4	6.154015661410402E-4	7.240018425188709E-5	7.569110171788195E-5
50-51	0.02763054304449291	1.596094971007511E-4	6.269197772720222E-4	7.240018425188709E-5	7.733656045087938E-5
52-53	0.033982013753863005	1.711277082317331E-4	6.483107408009889E-4	7.569110171788195E-5	8.391839538286912E-5
54-55	0.041013058919961036	1.711277082317331E-4	6.664107868639607E-4	7.569110171788195E-5	8.885477158186142E-5
56-57	0.04864305106487014	1.7770954316372284E-4	6.878017503929273E-4	7.569110171788195E-5	9.21456890478563E-5
58-59	0.05780661074893285	1.7770954316372284E-4	7.05901796455899E-4	7.569110171788195E-5	9.21456890478563E-5
60-61	0.06957657706606349	1.7935500189672027E-4	7.322291361838581E-4	7.569110171788195E-5	9.379114778085373E-5
62-63	0.08356462175527468	1.8100046062971773E-4	8.177929902997245E-4	7.569110171788195E-5	1.020184414458409E-4
64-65	0.10075143822143287	1.8593683682871002E-4	8.638658348236527E-4	7.569110171788195E-5	1.1024573511082806E-4
66-67	0.12037024269496127	1.991005066926895E-4	8.68802211022645E-4	7.569110171788195E-5	1.2011848750881267E-4
68-69	0.14353665619683215	2.040368828916818E-4	8.80320422153627E-4	7.733656045087938E-5	1.217639462418101E-4
70-71	0.1711309991491991	2.2049147022165611E-4	8.885477158186142E-4	7.898201918387681E-5	1.36573074838787E-4
72-73	0.20593245135209481	2.336551400856356E-4	9.017113856825937E-4	7.898201918387681E-5	1.5302766216876133E-4
74-75	0.2485794507939223	2.3859151628462792E-4	9.050023031485886E-4	8.227293664987169E-5	1.5796403836775363E-4
76-77	0.30024356409257574	2.468188099496151E-4	9.132295968135758E-4	8.391839538286912E-5	1.5796403836775363E-4
78-79	0.36072075436516343	2.5504610361460223E-4	9.181659730125681E-4	8.720931284886399E-5	1.5796403836775363E-4
80-81	0.4340341682138641	2.6162793854659196E-4	9.247478079445578E-4	9.21456890478563E-5	1.5796403836775363E-4
82-83	0.5232937226440428	2.73146149677574E-4	9.362660190755398E-4	9.543660651385116E-5	1.5796403836775363E-4
84-85	0.6326657191676492	2.7643706714356885E-4	9.44493312740527E-4	9.543660651385116E-5	1.596094971007511E-4
86-87	0.7678368631659225	2.830189020755586E-4	9.593024413375039E-4	9.872752397984602E-5	1.6454587329974338E-4
88-89	0.927854434032457	2.978280306725355E-4	9.691751937354884E-4	9.872752397984602E-5	1.6783679076573825E-4
90	1.0654444023682363	3.0934624180351753E-4	9.741115699344808E-4	9.872752397984602E-5	1.7770954316372284E-4
>>END_MODULE
