##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	SRR19654128_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	30717334
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.620754034187993	32.0	32.0	32.0	32.0	32.0
2	31.67517916756708	32.0	32.0	32.0	32.0	32.0
3	35.69037055754904	37.0	37.0	37.0	32.0	37.0
4	36.339026134234174	37.0	37.0	37.0	37.0	37.0
5	36.41807059167309	37.0	37.0	37.0	37.0	37.0
6	40.050340534110155	41.0	41.0	41.0	37.0	41.0
7	40.06066896951408	41.0	41.0	41.0	37.0	41.0
8	40.20991252040297	41.0	41.0	41.0	37.0	41.0
9	40.1839781082564	41.0	41.0	41.0	37.0	41.0
10-11	40.25345661508254	41.0	41.0	41.0	41.0	41.0
12-13	40.222712687240374	41.0	41.0	41.0	41.0	41.0
14-15	40.24750919139011	41.0	41.0	41.0	41.0	41.0
16-17	40.24572970753256	41.0	41.0	41.0	41.0	41.0
18-19	40.23589874368655	41.0	41.0	41.0	41.0	41.0
20-21	40.161070814283555	41.0	41.0	41.0	39.0	41.0
22-23	40.1112368019959	41.0	41.0	41.0	37.0	41.0
24-25	40.143955136210714	41.0	41.0	41.0	37.0	41.0
26-27	40.099580565813426	41.0	41.0	41.0	37.0	41.0
28-29	40.00305083442463	41.0	41.0	41.0	37.0	41.0
30-31	40.001904299377024	41.0	41.0	41.0	37.0	41.0
32-33	39.99430054704617	41.0	41.0	41.0	37.0	41.0
34-35	39.97209788779195	41.0	41.0	41.0	37.0	41.0
36-37	39.930383248754595	41.0	41.0	41.0	37.0	41.0
38-39	39.88081343582747	41.0	41.0	41.0	37.0	41.0
40-41	39.84290763970597	41.0	41.0	41.0	37.0	41.0
42-43	39.77152050369996	41.0	41.0	41.0	37.0	41.0
44-45	39.7587720991672	41.0	41.0	41.0	37.0	41.0
46-47	39.72102766145005	41.0	41.0	41.0	37.0	41.0
48-49	39.67143797375124	41.0	41.0	41.0	37.0	41.0
50-51	39.56509410940416	41.0	41.0	41.0	37.0	41.0
52-53	39.54194919715363	41.0	41.0	41.0	37.0	41.0
54-55	39.45459861523139	41.0	41.0	41.0	37.0	41.0
56-57	39.41179441223643	41.0	41.0	41.0	37.0	41.0
58-59	39.35119414985688	41.0	41.0	41.0	37.0	41.0
60-61	39.201221271351216	41.0	41.0	41.0	37.0	41.0
62-63	39.06043063502842	41.0	41.0	41.0	37.0	41.0
64-65	38.939235172557616	41.0	41.0	41.0	34.5	41.0
66-67	38.81105430568942	41.0	41.0	41.0	32.0	41.0
68-69	38.59921147453747	41.0	41.0	41.0	32.0	41.0
70-71	38.51139885707529	41.0	37.0	41.0	32.0	41.0
72-73	38.27247009782815	41.0	37.0	41.0	32.0	41.0
74-75	38.19731665189433	41.0	37.0	41.0	32.0	41.0
76-77	36.43528766200869	39.0	34.5	41.0	27.0	41.0
78-79	37.183449856032425	41.0	37.0	41.0	29.5	41.0
80-81	37.94661890253887	41.0	37.0	41.0	32.0	41.0
82-83	38.160730599211504	41.0	37.0	41.0	32.0	41.0
84-85	38.09528784301398	41.0	37.0	41.0	32.0	41.0
86-87	38.075357239661486	41.0	37.0	41.0	32.0	41.0
88-89	37.96968539001464	41.0	37.0	41.0	32.0	41.0
90-91	37.90219702985942	41.0	37.0	41.0	32.0	41.0
92-93	37.856116370645964	41.0	37.0	41.0	32.0	41.0
94-95	37.7007758225372	41.0	37.0	41.0	27.0	41.0
96-97	37.626261136464514	41.0	37.0	41.0	27.0	41.0
98-99	37.71387795242907	41.0	37.0	41.0	32.0	41.0
100-101	36.798050117239995	41.0	34.5	41.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	2.0
17	15.0
18	52.0
19	228.0
20	893.0
21	2829.0
22	6999.0
23	15121.0
24	28855.0
25	50015.0
26	80852.0
27	120613.0
28	167749.0
29	219869.0
30	276865.0
31	337452.0
32	407432.0
33	491419.0
34	593445.0
35	740506.0
36	989546.0
37	1473567.0
38	2662001.0
39	7231976.0
40	1.4819033E7
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.94364875472496	11.116414985557743	6.804332494700251	50.135603765017045
2	22.288669322669737	12.923165792968883	34.538674482622746	30.249490401738637
3	20.394816815808298	14.474622048905678	20.892304651178385	44.23825648410764
4	23.804142401372147	21.723609723739838	20.2058098127096	34.266438062178416
5	25.02991307774301	26.81965824247638	23.8792337902762	24.271194889504407
6	20.65735587600148	30.870931051503366	25.266320312824025	23.20539275967114
7	16.23388279725057	25.57517198595425	39.13463323347007	19.056311983325116
8	18.368166325892734	24.253973342868886	32.43254118342432	24.945319147814065
9	18.675037355270607	22.21445189041256	34.76709574373085	24.343415010585986
10-11	21.043393145486753	30.93049712058172	25.34569484558563	22.680414888345904
12-13	21.62429156321838	25.02859710085714	27.47228942432632	25.874821911598154
14-15	21.14184453637806	25.81119995635038	27.33524335152263	25.711712155748934
16-17	21.88504987955079	26.072761693593637	26.57058217448088	25.471606252374695
18-19	21.58979358494251	26.270079945318127	26.436518681316105	25.70360778842326
20-21	21.851363924194555	26.220827716368667	26.66408244784447	25.263725911592307
22-23	21.74504141537804	26.376983106671954	26.685517369443584	25.192458108506422
24-25	21.65908994576157	26.070789541826773	26.578517523688745	25.691602988722916
26-27	21.559063361423227	26.25714523272104	26.766826509097434	25.416964896758294
28-29	21.586225263167456	26.48998194875773	26.50932285614579	25.414469931929023
30-31	21.718684961396715	26.067032705377365	26.536487508974577	25.67779482425135
32-33	21.85043316479535	26.062737305641658	26.6453842266211	25.44144530294189
34-35	21.941132069230942	26.205644784806083	26.354322402234686	25.498900743728285
36-37	21.790458270321018	25.92212277362378	26.315991571955504	25.971427384099698
38-39	21.879759528694052	26.0747130145207	26.592988269897262	25.452539186887986
40-41	21.985006762960673	26.349849381371733	26.352803741056025	25.312340114611576
42-43	21.966418049170542	25.899962542322196	26.420712487613674	25.71290692089359
44-45	21.963918070074662	25.948948428204044	26.656047315705028	25.431086186016266
46-47	21.961212264967045	26.061795905862244	26.39175044949889	25.58524137967182
48-49	21.884125699751262	25.815248575185894	26.395292338990267	25.905333386072577
50-51	21.902843055597902	25.883875515769677	26.58808244683972	25.625198981792703
52-53	22.34106644801922	26.016785831739174	26.134790864337383	25.507356855904227
54-55	22.120591614379098	25.828331377770265	26.20510234545649	25.845974662394145
56-57	22.006481736902735	25.83251038313856	26.657480616863577	25.503527263095133
58-59	22.109287799200107	25.91068486194582	26.46302562171542	25.517001717138655
60-61	22.088371514983855	25.667461251283175	26.437278071979453	25.806889161753517
62-63	22.073582514985603	25.716630791849244	26.620626707236077	25.589159985929072
64-65	22.196364354245326	25.912554415955864	26.394142014388688	25.496939215410126
66-67	22.055590856649797	25.722907918576947	26.379159926848928	25.842341297924325
68-69	22.11940354535574	25.781368974888814	26.56901382111202	25.53021365864342
70-71	22.344387006236904	25.96708819304256	26.336370556470523	25.352154244250013
72-73	22.32553759732462	25.770972125065466	26.19435827504945	25.709132002560466
74-75	22.23859917344334	25.830941453644623	26.48984637977002	25.440612993142008
76-77	22.194823287724123	25.956153942265953	26.339111981528085	25.50991078848184
78-79	22.128622153063084	25.730191281891994	26.398124525322643	25.743062039722282
80-81	22.271716818220636	25.753059575414472	26.521755748962857	25.45346785740204
82-83	22.348887965545266	26.04688731685341	26.22720411776891	25.377020599832417
84-85	22.409694266333577	25.75991808652657	26.20998707210216	25.62040057503769
86-87	22.33514664694779	25.80688179889844	26.426483750055123	25.43148780409864
88-89	22.52766284880488	25.817433190131638	26.337928477458668	25.316975483604814
90-91	22.391105938813634	25.637495439563416	26.31656892455802	25.65482969706493
92-93	22.366762304419552	25.70695462418543	26.378555596197018	25.547727475197995
94-95	22.5962802246218	25.86383510908029	26.126668135197978	25.41321653109993
96-97	22.508630232270594	25.7918509537612	26.018826111310673	25.68069270265753
98-99	22.48948859569299	25.941096744311192	26.29920192191793	25.270212738077884
100-101	22.514767815439413	26.11675720622133	26.163203609489514	25.20527136884974
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	29.0
1	25.0
2	15.5
3	23.0
4	44.0
5	58.5
6	73.0
7	94.0
8	115.0
9	142.0
10	195.0
11	246.5
12	298.5
13	337.5
14	381.5
15	464.0
16	555.5
17	714.5
18	874.5
19	1082.0
20	1470.0
21	1958.0
22	2660.5
23	3750.0
24	5630.0
25	8149.0
26	11578.5
27	17103.5
28	24617.5
29	35017.0
30	49365.5
31	68210.5
32	94150.5
33	126993.5
34	168000.5
35	224556.0
36	295785.5
37	378937.0
38	490190.0
39	645566.5
40	828532.0
41	1030529.0
42	1254316.0
43	1484978.0
44	1693582.5
45	1838833.5
46	1925007.0
47	1950340.0
48	1891264.5
49	1780906.5
50	1651877.5
51	1497929.0
52	1359394.0
53	1228614.5
54	1076194.0
55	926183.0
56	786482.5
57	646588.5
58	515035.5
59	418139.5
60	346850.0
61	302008.0
62	267743.0
63	230055.0
64	206159.5
65	188557.5
66	169168.0
67	146241.5
68	107724.5
69	76299.0
70	61827.5
71	46754.5
72	34140.5
73	29846.0
74	26004.5
75	18828.0
76	10936.5
77	3505.5
78	370.5
79	77.0
80	21.0
81	8.0
82	6.5
83	4.0
84	2.0
85	2.5
86	1.5
87	1.5
88	2.0
89	1.5
90	1.0
91	0.5
92	0.5
93	1.0
94	1.0
95	0.5
96	1.0
97	1.5
98	1.5
99	2.0
100	3.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03327762754410914
2	0.0
3	0.0
4	0.018803715192210364
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0025555603230410554
10-11	0.003169220349656647
12-13	0.0016163512106877505
14-15	0.0
16-17	7.715513331983823E-4
18-19	8.30150168631171E-5
20-21	3.93914393742634E-4
22-23	0.0
24-25	0.0
26-27	0.0
28-29	1.871907242991856E-4
30-31	0.0
32-33	0.002011893349859073
34-35	6.576091531901824E-4
36-37	4.085641026008312E-4
38-39	0.0042972479317378255
40-41	5.859883543278854E-5
42-43	0.0
44-45	0.002498589233036956
46-47	2.360230871598427E-4
48-49	5.501779548967368E-4
50-51	4.39491265745914E-5
52-53	0.0
54-55	8.610773317762538E-4
56-57	3.4019879459591126E-4
58-59	7.992230054860881E-4
60-61	8.627050772049423E-5
62-63	3.4019879459591126E-4
64-65	0.002278843600163999
66-67	0.0020444482584328445
68-69	0.008058967617437112
70-71	0.008107799980297769
72-73	7.210912249090367E-4
74-75	0.0012484807438041335
76-77	0.0
78-79	0.0013575396875262677
80-81	0.008246158341736297
82-83	0.005441552968105891
84-85	1.3998610686721705E-4
86-87	0.009563004393545353
88-89	1.7254101544098845E-4
90-91	0.0022869823273074415
92-93	6.852808254778882E-4
94-95	0.013375184187533983
96-97	0.0010287351109311766
98-99	3.304323220237798E-4
100-101	0.014696913475629103
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3.0717334E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.63089514692629
#Duplication Level	Percentage of deduplicated	Percentage of total
1	63.37506417941544	20.679850741682298
2	15.3555280797041	10.021292533890138
3	6.630962282887726	6.491227049283971
4	3.6988973899936632	4.827933315684902
5	2.2646267738330748	3.6948399401934515
6	1.5954182205653915	3.1235954802459007
7	1.1318935352715962	2.585428948685182
8	0.8414500632335256	2.1965815027798135
9	0.6764972101418201	1.98672385781933
>10	3.970431324412491	23.966950709389156
>50	0.2918577749410792	6.562088995636079
>100	0.1536398731360727	9.112881074936318
>500	0.009477435879908971	2.086913247521939
>1k	0.004172416987658123	2.482251293397393
>5k	8.34395962487986E-5	0.18144130885399287
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.6277454286885704E-5	0.0	3.2554908573771408E-6	0.0	3.2554908573771408E-6
2	1.6277454286885704E-5	6.5109817147542815E-6	6.5109817147542815E-6	3.2554908573771408E-6	6.5109817147542815E-6
3	4.557687200327997E-5	6.5109817147542815E-6	6.5109817147542815E-6	3.2554908573771408E-6	1.3021963429508563E-5
4	7.16207988622971E-5	6.5109817147542815E-6	6.5109817147542815E-6	3.2554908573771408E-6	1.3021963429508563E-5
5	1.0092021657869137E-4	6.5109817147542815E-6	6.5109817147542815E-6	3.2554908573771408E-6	1.3021963429508563E-5
6	1.3673061600983993E-4	6.5109817147542815E-6	1.6277454286885704E-5	3.2554908573771408E-6	1.3021963429508563E-5
7	1.5626356115410275E-4	6.5109817147542815E-6	1.6277454286885704E-5	3.2554908573771408E-6	1.3021963429508563E-5
8	1.660300337262342E-4	6.5109817147542815E-6	1.6277454286885704E-5	3.2554908573771408E-6	1.3021963429508563E-5
9	1.823074880131199E-4	1.3021963429508563E-5	1.9532945144262844E-5	3.2554908573771408E-6	2.6043926859017126E-5
10-11	2.1811788744426845E-4	3.581039943114855E-5	2.1160690572951417E-5	3.2554908573771408E-6	3.418265400245998E-5
12-13	2.588115231614827E-4	3.906589028852569E-5	2.2788436001639987E-5	8.138727143442852E-6	4.720461743196854E-5
14-15	3.206658494516484E-4	4.069363571721426E-5	2.2788436001639987E-5	1.3021963429508563E-5	5.0460108289345686E-5
16-17	3.955421391713226E-4	4.2321381145902834E-5	2.2788436001639987E-5	1.3021963429508563E-5	5.208785371803425E-5
18-19	4.687906834623083E-4	4.394912657459141E-5	2.2788436001639987E-5	1.3021963429508563E-5	6.185432629016567E-5
20-21	5.257617734664082E-4	4.557687200327997E-5	2.2788436001639987E-5	1.3021963429508563E-5	6.510981714754282E-5
22-23	6.169155174729682E-4	4.720461743196854E-5	2.2788436001639987E-5	1.3021963429508563E-5	7.16207988622971E-5
24-25	7.259744611951024E-4	6.0226580861477104E-5	2.2788436001639987E-5	1.3021963429508563E-5	7.324854429098567E-5
26-27	8.627050772049424E-4	9.766472572131423E-5	2.6043926859017126E-5	1.3021963429508563E-5	7.487628971967424E-5
28-29	0.0010515235469328165	9.766472572131423E-5	2.6043926859017126E-5	1.3021963429508563E-5	7.650403514836282E-5
30-31	0.0013363789969533164	9.766472572131423E-5	2.7671672287705696E-5	1.3021963429508563E-5	8.301501686311708E-5
32-33	0.0017563373175549673	1.0580345286475708E-4	3.0927163145082835E-5	1.3021963429508563E-5	1.0417570743606852E-4
34-35	0.0022332667281607186	1.1394218000819993E-4	3.255490857377141E-5	1.3021963429508563E-5	1.0743119829344565E-4
36-37	0.002874598427064015	1.1719767086557708E-4	3.255490857377141E-5	1.3021963429508563E-5	1.0905894372213422E-4
38-39	0.0038024133214165003	1.1719767086557708E-4	3.255490857377141E-5	1.3021963429508563E-5	1.2533639800901993E-4
40-41	0.005016711411218174	1.1719767086557708E-4	3.255490857377141E-5	1.3021963429508563E-5	1.3835836143852849E-4
42-43	0.006476799060751822	1.3998610686721705E-4	3.581039943114855E-5	1.3021963429508563E-5	1.4161385229590563E-4
44-45	0.008213603433162527	1.6765777915492275E-4	3.906589028852569E-5	1.3021963429508563E-5	1.4975257943934848E-4
46-47	0.010204336092448647	1.8556297887049704E-4	3.906589028852569E-5	1.3021963429508563E-5	1.5138032486803704E-4
48-49	0.012675253653197898	1.8556297887049704E-4	3.906589028852569E-5	1.3021963429508563E-5	1.6765777915492275E-4
50-51	0.015842846257425856	1.920739605852513E-4	3.906589028852569E-5	1.3021963429508563E-5	1.8881846972787416E-4
52-53	0.01992685953800548	1.9532945144262845E-4	3.906589028852569E-5	1.3021963429508563E-5	1.9532945144262845E-4
54-55	0.02469615364406299	2.034681785860713E-4	3.906589028852569E-5	1.3021963429508563E-5	1.9695719687131704E-4
56-57	0.03025490428303446	2.3276759630246556E-4	3.906589028852569E-5	1.3021963429508563E-5	2.034681785860713E-4
58-59	0.03663892185435103	2.4416181430328556E-4	5.6971090004099965E-5	1.3021963429508563E-5	2.1811788744426845E-4
60-61	0.044694633980930765	2.6043926859017127E-4	6.0226580861477104E-5	1.3021963429508563E-5	2.2625661458771127E-4
62-63	0.055320556139409754	2.750889774483684E-4	6.185432629016567E-5	1.3021963429508563E-5	2.3765083258853127E-4
64-65	0.06799743753803633	2.799722137344341E-4	6.185432629016567E-5	1.3021963429508563E-5	2.3927857801721986E-4
66-67	0.08321848504170316	2.815999591631227E-4	6.185432629016567E-5	1.3021963429508563E-5	2.5555603230410556E-4
68-69	0.10179268812846845	2.8322770459181126E-4	6.673756257623139E-5	1.3021963429508563E-5	2.620670140188598E-4
70-71	0.12454694147610598	2.8648319544918844E-4	6.836530800491996E-5	1.3021963429508563E-5	2.669502503049255E-4
72-73	0.15263043335726986	2.8973868630656556E-4	7.487628971967424E-5	1.3021963429508563E-5	2.750889774483684E-4
74-75	0.18783042825266022	3.043883951647627E-4	7.487628971967424E-5	1.3021963429508563E-5	2.799722137344341E-4
76-77	0.23096730985833602	3.727537031696826E-4	7.487628971967424E-5	1.3021963429508563E-5	2.8648319544918844E-4
78-79	0.2829542433597916	3.82520175741814E-4	7.487628971967424E-5	1.3021963429508563E-5	2.9136643173525414E-4
80-81	0.34651444685922284	3.874034120278798E-4	7.487628971967424E-5	1.3021963429508563E-5	2.962496680213198E-4
82-83	0.42435323325911034	3.874034120278798E-4	7.487628971967424E-5	1.3021963429508563E-5	3.0113290430738556E-4
84-85	0.5211047937949302	4.05308611743454E-4	7.487628971967424E-5	1.3021963429508563E-5	3.1089937687951697E-4
86-87	0.6422725357610788	4.297247931737826E-4	7.813178057705137E-5	1.3021963429508563E-5	3.125271223082055E-4
88-89	0.7880827157721435	4.7041842889099684E-4	7.975952600573995E-5	1.3021963429508563E-5	3.125271223082055E-4
90	0.9134126027994487	4.883236286065711E-4	8.464276229180567E-5	1.3021963429508563E-5	3.190381040229598E-4
>>END_MODULE
