##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	SRR19654114_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	34533747
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.610672858638825	32.0	32.0	32.0	32.0	32.0
2	31.607318458665954	32.0	32.0	32.0	32.0	32.0
3	35.68937260124133	37.0	37.0	37.0	32.0	37.0
4	36.3157797212101	37.0	37.0	37.0	37.0	37.0
5	36.506266869911336	37.0	37.0	37.0	37.0	37.0
6	40.065677205546216	41.0	41.0	41.0	37.0	41.0
7	40.10264379941163	41.0	41.0	41.0	37.0	41.0
8	40.1982820456755	41.0	41.0	41.0	37.0	41.0
9	40.213269675022524	41.0	41.0	41.0	37.0	41.0
10-11	40.27477433885179	41.0	41.0	41.0	41.0	41.0
12-13	40.24961006982532	41.0	41.0	41.0	41.0	41.0
14-15	40.20197058257246	41.0	41.0	41.0	41.0	41.0
16-17	40.227145015569846	41.0	41.0	41.0	41.0	41.0
18-19	40.22026380456195	41.0	41.0	41.0	41.0	41.0
20-21	40.1990892995191	41.0	41.0	41.0	41.0	41.0
22-23	40.191232868532914	41.0	41.0	41.0	41.0	41.0
24-25	40.17462350668174	41.0	41.0	41.0	41.0	41.0
26-27	40.12180948681879	41.0	41.0	41.0	39.0	41.0
28-29	40.10002604119385	41.0	41.0	41.0	37.0	41.0
30-31	40.077769174598984	41.0	41.0	41.0	37.0	41.0
32-33	40.06974887491937	41.0	41.0	41.0	37.0	41.0
34-35	40.05315163164889	41.0	41.0	41.0	37.0	41.0
36-37	39.945160222549845	41.0	41.0	41.0	37.0	41.0
38-39	39.98135926286829	41.0	41.0	41.0	37.0	41.0
40-41	39.990471537884375	41.0	41.0	41.0	37.0	41.0
42-43	39.973479173864334	41.0	41.0	41.0	37.0	41.0
44-45	39.957156850080594	41.0	41.0	41.0	37.0	41.0
46-47	39.93882331969363	41.0	41.0	41.0	37.0	41.0
48-49	39.916988199977254	41.0	41.0	41.0	37.0	41.0
50-51	39.85261542861248	41.0	41.0	41.0	37.0	41.0
52-53	39.85036775766035	41.0	41.0	41.0	37.0	41.0
54-55	39.808787256708634	41.0	41.0	41.0	37.0	41.0
56-57	39.79351326978795	41.0	41.0	41.0	37.0	41.0
58-59	39.760412734824286	41.0	41.0	41.0	37.0	41.0
60-61	39.76134113103916	41.0	41.0	41.0	37.0	41.0
62-63	39.71310752928143	41.0	41.0	41.0	37.0	41.0
64-65	39.721714950306435	41.0	41.0	41.0	37.0	41.0
66-67	39.66339913824005	41.0	41.0	41.0	37.0	41.0
68-69	39.6286185654861	41.0	41.0	41.0	37.0	41.0
70-71	39.62656257660079	41.0	41.0	41.0	37.0	41.0
72-73	39.59887544204224	41.0	41.0	41.0	37.0	41.0
74-75	39.55373307738659	41.0	41.0	41.0	37.0	41.0
76-77	38.99176892099198	41.0	39.0	41.0	34.5	41.0
78-79	39.361310589899205	41.0	41.0	41.0	37.0	41.0
80-81	39.64982092733812	41.0	41.0	41.0	37.0	41.0
82-83	39.67314492400723	41.0	41.0	41.0	37.0	41.0
84-85	39.68694795847088	41.0	41.0	41.0	37.0	41.0
86-87	39.669597263801116	41.0	41.0	41.0	37.0	41.0
88-89	39.62824585759547	41.0	41.0	41.0	37.0	41.0
90-91	39.5927241547232	41.0	41.0	41.0	37.0	41.0
92-93	39.544750733246524	41.0	41.0	41.0	37.0	41.0
94-95	39.52295052141316	41.0	41.0	41.0	37.0	41.0
96-97	39.500736554883545	41.0	41.0	41.0	37.0	41.0
98-99	39.45528779428424	41.0	41.0	41.0	37.0	41.0
100-101	38.91742063205594	41.0	39.0	41.0	34.5	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	2.0
15	3.0
16	9.0
17	18.0
18	51.0
19	203.0
20	638.0
21	1947.0
22	4608.0
23	9841.0
24	19023.0
25	32814.0
26	53541.0
27	83697.0
28	120354.0
29	164843.0
30	215772.0
31	271918.0
32	335425.0
33	407489.0
34	488735.0
35	593210.0
36	740585.0
37	973981.0
38	1466923.0
39	3306586.0
40	2.5241531E7
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.902988765583615	10.583625666358838	5.695074844553441	43.818310723504105
2	24.990048140446504	12.405416070257305	30.481363635402786	32.12317215389341
3	21.49068743499509	14.064308462113898	20.359026781567884	44.08597732132313
4	24.74615048288852	21.171904687898476	20.356942442417267	33.72500238679573
5	25.51051873982861	25.939956645886124	23.347961633007852	25.201562981277416
6	21.295412860932814	30.417350888682886	25.039463571676713	23.247772678707584
7	16.546339440084505	25.04612951499297	39.98865805092045	18.41887299400207
8	19.013807800442535	24.652682409296425	31.882562214821796	24.450947575439248
9	19.022022139676878	22.556506827944272	34.67181826518854	23.74965276719031
10-11	21.40600757861578	30.909026466198412	25.745558395386404	21.939407559799402
12-13	22.064493899257442	25.204890161499126	27.89592018497153	24.834695754271902
14-15	21.670885270994333	25.965520375318114	27.369016349908527	24.99457800377903
16-17	22.155449856049504	26.343152105677962	26.72774402383848	24.773654014434054
18-19	21.96340292873519	26.534892086862165	26.486824612458072	25.01488037194458
20-21	22.174962653198335	26.416781532568706	26.688024905029856	24.720230909203107
22-23	22.10315318520171	26.563908631171707	26.61351083622637	24.71942734740021
24-25	22.076589457116953	26.257951976674743	26.541282228946507	25.124176337261805
26-27	21.94160106634244	26.37793112922267	26.608066885994152	25.072400918440735
28-29	21.911430001260804	26.50189140858555	26.514823770364345	25.07185481978931
30-31	22.02830031736782	26.336002577420864	26.350837341803658	25.284859763407656
32-33	22.061182475286127	26.377635845750298	26.417308635365206	25.14387304359837
34-35	22.33910395895814	26.42778603677487	26.236465782378293	24.996644221888698
36-37	22.036002927802766	26.264844646021178	26.25865939192755	25.4404930342485
38-39	21.972166819893594	26.30330267954995	26.562654785187373	25.161875715369085
40-41	22.262873762294024	26.69384095505188	26.13762271438428	24.905662568269815
42-43	22.26684668767626	26.39500717949894	26.25477478595068	25.083371346874117
44-45	22.26510056959646	26.44902390696266	26.342512151953855	24.943363371487028
46-47	22.133869516099715	26.31818377542408	26.435758621848944	25.112188086627263
48-49	22.00656505649387	26.15539663274883	26.35129631313972	25.486741997617578
50-51	22.04582954754374	26.175203345295834	26.523611816580463	25.255355290579967
52-53	22.483465231849877	26.519347871518256	25.968001676736673	25.029185219895194
54-55	22.409951633687477	26.412708704908272	25.932555190144875	25.244784471259372
56-57	22.177228552696583	26.25829742715148	26.47788263462983	25.086591385522112
58-59	22.157427631585996	26.289058641681716	26.493817771931905	25.059695954800386
60-61	22.314511657249355	25.99409064993729	26.455414757049095	25.235982935764255
62-63	22.15042288923933	26.139454256151236	26.40239560450825	25.307727250101188
64-65	22.243523125364877	26.40065672572397	26.09110010564449	25.264720043266664
66-67	22.32404870516947	26.208298508702228	26.05436502444985	25.413287761678454
68-69	22.28464666750469	26.360199198772143	26.412010836819995	24.943143296903173
70-71	22.520785248122653	26.653276286526335	26.14780985104223	24.678128614308783
72-73	22.615339564679324	26.087998728779354	26.05536831916904	25.241293387372277
74-75	22.478347900883794	26.017340154082934	26.402645219269477	25.101666725763792
76-77	22.282124496944974	26.27052749300561	26.43382862566289	25.013519384386523
78-79	22.284820421479225	26.203397810545177	26.200920484987662	25.31086128298794
80-81	22.412960284906237	26.21948177242394	26.341155507973113	25.026402434696703
82-83	22.66940219374399	26.409375733250144	25.97008948956509	24.95113258344077
84-85	22.525437219424813	26.207053349872517	26.014524285476465	25.252985145226205
86-87	22.44996279372463	26.08515359139086	26.327005240442425	25.137878374442092
88-89	22.63621196121485	26.072061193036966	26.276148599399896	25.015578246348287
90-91	22.621770524930294	25.945464301918932	26.297899269372653	25.13486590377812
92-93	22.505526209592418	25.917547991499067	26.4038987402883	25.173027058620217
94-95	22.7016510951676	26.008059107009984	26.253968623062935	25.036321174759486
96-97	22.723930015471534	25.935154097237117	26.111669839939466	25.229246047351882
98-99	22.608880235324595	26.19140778439131	26.20378408401498	24.99592789626912
100-101	22.713198483790364	26.355888922218607	26.016738062046958	24.914174531944074
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	113.0
1	84.0
2	55.5
3	51.0
4	57.0
5	82.0
6	109.0
7	123.5
8	142.0
9	171.0
10	198.5
11	247.5
12	313.5
13	353.5
14	389.0
15	456.5
16	515.0
17	617.5
18	764.5
19	922.0
20	1262.5
21	1701.5
22	2526.5
23	3793.0
24	5468.5
25	7949.5
26	11960.0
27	17853.0
28	25084.0
29	35694.5
30	50845.0
31	70173.0
32	97253.5
33	132779.5
34	174206.0
35	233759.5
36	322158.5
37	432053.0
38	569361.0
39	761552.0
40	989664.5
41	1225189.0
42	1459578.5
43	1682374.0
44	1888453.0
45	2056201.5
46	2171378.0
47	2180678.5
48	2095747.5
49	1989938.5
50	1873109.5
51	1718707.5
52	1555924.0
53	1364318.0
54	1177186.0
55	1045291.0
56	908363.5
57	761198.0
58	623160.0
59	498060.5
60	395351.5
61	347411.5
62	308675.0
63	258339.0
64	211234.5
65	170874.5
66	148197.0
67	122649.0
68	90791.5
69	67073.0
70	49883.5
71	36678.5
72	28992.0
73	25453.5
74	20279.5
75	13034.0
76	7650.0
77	2809.5
78	455.0
79	136.0
80	49.0
81	23.0
82	9.5
83	4.5
84	3.0
85	4.0
86	3.0
87	1.5
88	1.5
89	2.0
90	1.5
91	1.0
92	1.0
93	1.5
94	1.5
95	1.0
96	1.0
97	0.5
98	0.5
99	0.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.023325010170486278
2	0.0
3	0.0022586602027286524
4	0.0
5	0.0
6	0.0
7	0.0
8	8.571325897534373E-4
9	0.0
10-11	0.0
12-13	0.0
14-15	0.002320918144214122
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	5.646650506821631E-4
26-27	0.0
28-29	6.515365969409575E-4
30-31	0.0
32-33	5.791436417252956E-6
34-35	7.963225073722814E-5
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	2.750932298195154E-5
74-75	5.834872190382352E-4
76-77	0.0
78-79	0.0015593442553453582
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0017200566159241279
88-89	4.0540054920770685E-5
90-91	0.0
92-93	3.793390853300686E-4
94-95	0.0014956384547555757
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3.4533747E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.01730306432922
#Duplication Level	Percentage of deduplicated	Percentage of total
1	60.44985879150131	14.518425787913927
2	15.539968349422425	7.464562589163245
3	6.938636385497456	4.999419987710227
4	3.8840227308940647	3.7313500414650544
5	2.4668078038557204	2.9623035313327604
6	1.7122122317255117	2.467363204788186
7	1.2839998034834839	2.158674869036139
8	0.9928690570187639	1.9076829636491532
9	0.7723337502336777	1.6694436371554964
>10	5.116676850264625	23.591525719240884
>50	0.48534547454814525	8.079347387330415
>100	0.3149813279902454	14.66461232736832
>500	0.026678725000870752	4.383385390847381
>1k	0.015015908672707152	6.391353395405523
>5k	5.675839381999755E-4	0.9136056124081829
>10k+	2.52259528088878E-5	0.096943555185141
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.764433671214421E-5	0.0	0.0	5.791436417252956E-6	0.0
2	4.922720954665012E-5	0.0	0.0	5.791436417252956E-6	0.0
3	6.660151879840899E-5	0.0	0.0	5.791436417252956E-6	2.895718208626478E-6
4	1.1582872834505911E-4	0.0	5.791436417252956E-6	5.791436417252956E-6	5.791436417252956E-6
5	1.3899447401407092E-4	0.0	5.791436417252956E-6	8.687154625879432E-6	5.791436417252956E-6
6	1.9401311997797402E-4	0.0	2.0270027460385346E-5	8.687154625879432E-6	5.791436417252956E-6
7	2.084917110211064E-4	0.0	2.0270027460385346E-5	8.687154625879432E-6	5.791436417252956E-6
8	2.4324032952462412E-4	0.0	2.0270027460385346E-5	8.687154625879432E-6	8.687154625879432E-6
9	2.750932298195154E-4	0.0	2.3165745669011823E-5	8.687154625879432E-6	1.4478591043132389E-5
10-11	3.0260255280146694E-4	0.0	2.3165745669011823E-5	8.687154625879432E-6	2.461360477332506E-5
12-13	3.4459046682655086E-4	2.895718208626478E-6	2.3165745669011823E-5	1.158287283450591E-5	3.330075939920449E-5
14-15	3.88026239955948E-4	2.895718208626478E-6	2.3165745669011823E-5	1.4478591043132389E-5	3.6196477607830974E-5
16-17	4.5173204054573055E-4	2.895718208626478E-6	2.3165745669011823E-5	1.4478591043132389E-5	3.909219581645745E-5
18-19	5.125421229268865E-4	2.895718208626478E-6	2.3165745669011823E-5	1.4478591043132389E-5	4.054005492077069E-5
20-21	5.762479235166691E-4	2.895718208626478E-6	2.3165745669011823E-5	1.4478591043132389E-5	4.3435773129397166E-5
22-23	6.500887378366443E-4	2.895718208626478E-6	2.3165745669011823E-5	1.4478591043132389E-5	4.3435773129397166E-5
24-25	7.224816930523062E-4	5.791436417252956E-6	2.60614638776383E-5	1.4478591043132389E-5	4.3435773129397166E-5
26-27	8.383104213973653E-4	1.4478591043132389E-5	2.60614638776383E-5	1.4478591043132389E-5	4.3435773129397166E-5
28-29	0.001006262077497701	1.4478591043132389E-5	2.60614638776383E-5	1.7374309251758865E-5	4.633149133802364E-5
30-31	0.0012726681526913368	1.4478591043132389E-5	3.474861850351773E-5	1.7374309251758865E-5	5.21229277552766E-5
32-33	0.0016216021968308274	1.4478591043132389E-5	3.474861850351773E-5	1.7374309251758865E-5	5.21229277552766E-5
34-35	0.0021312486015490875	2.0270027460385346E-5	3.474861850351773E-5	1.7374309251758865E-5	5.21229277552766E-5
36-37	0.002788576634907298	2.0270027460385346E-5	3.764433671214421E-5	1.7374309251758865E-5	5.21229277552766E-5
38-39	0.0037687772485273606	2.0270027460385346E-5	3.764433671214421E-5	1.7374309251758865E-5	6.949723700703546E-5
40-41	0.005029862528384192	2.0270027460385346E-5	3.764433671214421E-5	1.7374309251758865E-5	7.528867342428842E-5
42-43	0.006718066244013428	2.3165745669011823E-5	3.764433671214421E-5	1.7374309251758865E-5	7.81843916329149E-5
44-45	0.008739277553634711	3.33007593992045E-5	4.3435773129397166E-5	1.7374309251758865E-5	7.81843916329149E-5
46-47	0.011035582093075508	4.3435773129397166E-5	4.3435773129397166E-5	1.7374309251758865E-5	7.81843916329149E-5
48-49	0.013967496779309814	4.488363223371041E-5	4.3435773129397166E-5	1.7374309251758865E-5	7.81843916329149E-5
50-51	0.017798531969322646	4.6331491338023646E-5	4.488363223371041E-5	1.7374309251758865E-5	7.81843916329149E-5
52-53	0.02255619698609595	4.6331491338023646E-5	4.6331491338023646E-5	1.8822168356072105E-5	7.81843916329149E-5
54-55	0.028317228362158324	4.922720954665012E-5	4.6331491338023646E-5	2.0270027460385346E-5	7.963225073722814E-5
56-57	0.03498751525572942	6.081008238115603E-5	4.6331491338023646E-5	2.0270027460385346E-5	8.397582805016786E-5
58-59	0.042795819405290716	6.081008238115603E-5	5.9362223276842793E-5	2.0270027460385346E-5	8.687154625879433E-5
60-61	0.052492131826876474	6.081008238115603E-5	6.081008238115603E-5	2.0270027460385346E-5	8.687154625879433E-5
62-63	0.06459768179803946	6.225794148546927E-5	6.081008238115603E-5	2.3165745669011823E-5	8.687154625879433E-5
64-65	0.0795844713867858	6.370580058978251E-5	6.081008238115603E-5	2.3165745669011823E-5	9.411084178036054E-5
66-67	0.09730192324626691	6.370580058978251E-5	6.370580058978251E-5	2.3165745669011823E-5	9.555870088467377E-5
68-69	0.11857097348862838	6.370580058978251E-5	6.370580058978251E-5	2.60614638776383E-5	9.845441909330024E-5
70-71	0.14374779545353128	6.370580058978251E-5	6.370580058978251E-5	2.60614638776383E-5	9.845441909330024E-5
72-73	0.17522135666309246	6.370580058978251E-5	6.370580058978251E-5	2.60614638776383E-5	9.990227819761348E-5
74-75	0.21499259839947282	6.370580058978251E-5	6.370580058978251E-5	2.60614638776383E-5	1.042458555105532E-4
76-77	0.2639736718983897	1.0279799640623996E-4	6.370580058978251E-5	2.60614638776383E-5	1.042458555105532E-4
78-79	0.32240202605295043	1.0714157371917968E-4	6.660151879840899E-5	2.7509322981951538E-5	1.0714157371917968E-4
80-81	0.3920454968295216	1.0714157371917968E-4	6.660151879840899E-5	2.8957182086264778E-5	1.0714157371917968E-4
82-83	0.4767191929679684	1.0714157371917968E-4	6.660151879840899E-5	2.8957182086264778E-5	1.1003729192780615E-4
84-85	0.5812039452307334	1.0858943282349292E-4	6.660151879840899E-5	2.8957182086264778E-5	1.1003729192780615E-4
86-87	0.7089109096675782	1.1003729192780615E-4	6.660151879840899E-5	2.8957182086264778E-5	1.1293301013643264E-4
88-89	0.8611417116132807	1.1582872834505911E-4	7.239295521566195E-5	2.8957182086264778E-5	1.1293301013643264E-4
90	0.9923394643506249	1.1582872834505911E-4	7.81843916329149E-5	2.8957182086264778E-5	1.1293301013643264E-4
>>END_MODULE
