##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	SRR19654107_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	29814926
Sequences flagged as poor quality	0
Sequence length	101
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.655529247330684	32.0	32.0	32.0	32.0	32.0
2	31.682021514995544	32.0	32.0	32.0	32.0	32.0
3	35.782937445492905	37.0	37.0	37.0	32.0	37.0
4	36.36661892100621	37.0	37.0	37.0	37.0	37.0
5	36.5461833445436	37.0	37.0	37.0	37.0	37.0
6	40.15326004833955	41.0	41.0	41.0	37.0	41.0
7	40.17898333874785	41.0	41.0	41.0	37.0	41.0
8	40.231591988522794	41.0	41.0	41.0	41.0	41.0
9	40.27418961227675	41.0	41.0	41.0	41.0	41.0
10-11	40.30458531072658	41.0	41.0	41.0	41.0	41.0
12-13	40.28966422723974	41.0	41.0	41.0	41.0	41.0
14-15	40.27770640785759	41.0	41.0	41.0	41.0	41.0
16-17	40.274334640307345	41.0	41.0	41.0	41.0	41.0
18-19	40.267495146558474	41.0	41.0	41.0	41.0	41.0
20-21	40.231826904416934	41.0	41.0	41.0	41.0	41.0
22-23	40.23330934982029	41.0	41.0	41.0	41.0	41.0
24-25	40.21541889790369	41.0	41.0	41.0	41.0	41.0
26-27	40.11728253157496	41.0	41.0	41.0	39.0	41.0
28-29	40.11247856191224	41.0	41.0	41.0	37.0	41.0
30-31	40.009007132870295	41.0	41.0	41.0	37.0	41.0
32-33	40.01176175986484	41.0	41.0	41.0	37.0	41.0
34-35	40.00979014672047	41.0	41.0	41.0	37.0	41.0
36-37	39.97681235230971	41.0	41.0	41.0	37.0	41.0
38-39	39.87445529799403	41.0	41.0	41.0	37.0	41.0
40-41	39.870540480295006	41.0	41.0	41.0	37.0	41.0
42-43	39.83367361032524	41.0	41.0	41.0	37.0	41.0
44-45	39.80567654268201	41.0	41.0	41.0	37.0	41.0
46-47	39.76229954419475	41.0	41.0	41.0	37.0	41.0
48-49	39.64577809114804	41.0	41.0	41.0	37.0	41.0
50-51	39.55944661744255	41.0	41.0	41.0	37.0	41.0
52-53	39.56586479537128	41.0	41.0	41.0	37.0	41.0
54-55	39.50524742204626	41.0	41.0	41.0	37.0	41.0
56-57	39.43538811734767	41.0	41.0	41.0	37.0	41.0
58-59	39.375543058533836	41.0	41.0	41.0	37.0	41.0
60-61	39.27738643389556	41.0	41.0	41.0	37.0	41.0
62-63	39.121385744844716	41.0	41.0	41.0	37.0	41.0
64-65	38.933448652530615	41.0	41.0	41.0	34.5	41.0
66-67	38.77929489075371	41.0	41.0	41.0	32.0	41.0
68-69	38.65340787698081	41.0	41.0	41.0	32.0	41.0
70-71	38.50814825098006	41.0	37.0	41.0	32.0	41.0
72-73	38.33647693775929	41.0	37.0	41.0	32.0	41.0
74-75	38.191550467037885	41.0	37.0	41.0	32.0	41.0
76-77	36.393376257247795	39.0	34.5	41.0	27.0	41.0
78-79	37.137810689183	41.0	37.0	41.0	29.5	41.0
80-81	37.88703391381887	41.0	37.0	41.0	32.0	41.0
82-83	38.034595407011906	41.0	37.0	41.0	32.0	41.0
84-85	38.062524186711045	41.0	37.0	41.0	32.0	41.0
86-87	38.044866537652986	41.0	37.0	41.0	32.0	41.0
88-89	37.903608967535256	41.0	37.0	41.0	32.0	41.0
90-91	37.859587593811234	41.0	37.0	41.0	32.0	41.0
92-93	37.82619752267706	41.0	37.0	41.0	32.0	41.0
94-95	37.832563746762276	41.0	37.0	41.0	32.0	41.0
96-97	37.80611825097268	41.0	37.0	41.0	32.0	41.0
98-99	37.788788373984225	41.0	37.0	41.0	32.0	41.0
100-101	36.85144286455717	41.0	34.5	41.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	2.0
17	16.0
18	47.0
19	187.0
20	695.0
21	2375.0
22	6113.0
23	13543.0
24	26358.0
25	46172.0
26	75079.0
27	112379.0
28	156238.0
29	205888.0
30	259165.0
31	316352.0
32	380414.0
33	458096.0
34	556149.0
35	699673.0
36	932191.0
37	1397371.0
38	2628107.0
39	7283347.0
40	1.4258969E7
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.548377003963296	12.175880780353102	8.236384097411207	39.03935811827239
2	23.125497611498346	14.156667703954723	34.22062828530918	28.49720639923775
3	19.50903014753761	18.659699950085372	25.03573548076925	36.79553442160777
4	22.35492719317834	26.245844111771397	23.621802717202787	27.77742597784747
5	23.216113298419724	30.93502563112181	24.32423947656285	21.52462159389562
6	20.220432544424224	33.17450460886604	24.63120653058136	21.97385631612837
7	15.891842226943645	23.95024894577971	41.02122540904512	19.136683418231527
8	18.99483164908744	24.334408879632974	30.12501188163271	26.54574758964688
9	18.504879066277073	22.97034042613421	33.20061904564177	25.324161461946947
10-11	21.785070201328878	30.84820368603472	23.914489436084136	23.452236676552264
12-13	21.574494265053684	24.758419658663584	27.554111655350077	26.112974420932655
14-15	20.793331836543885	26.11078591977723	27.923522265324426	25.172359978354464
16-17	21.418086021810847	26.59184227951126	27.137421233342433	24.85265046533546
18-19	21.25928513297365	26.957813706215344	26.923269912372334	24.85963124843867
20-21	21.424131658082935	26.780675222873267	27.065168633992247	24.730024485051548
22-23	21.513046854451357	27.023917483477906	26.936206381998062	24.526829280072672
24-25	21.479319077214196	26.701724202062373	26.718353475224614	25.10060324549882
26-27	21.194768351932183	26.77057122328595	27.033961110619558	25.00069931416231
28-29	21.360636408016344	26.936253378561375	26.95918491583133	24.743925297590945
30-31	21.416774529692137	26.79555692379666	26.89146349200296	24.896205054508247
32-33	21.43657508993985	26.63949425868104	26.93353993231444	24.990390719064674
34-35	21.5586716532518	26.778092623808625	26.684253719093586	24.978982003845992
36-37	21.468227021593144	26.67807057444986	26.669324954890044	25.184377449066954
38-39	21.555297590265084	26.685752945289607	26.868584293630178	24.89036517081513
40-41	21.76987831145176	26.847742752199732	26.782751813349325	24.599627122999184
42-43	21.660526677141508	26.575417292667435	26.678211443489747	25.085844586701306
44-45	21.623907434819728	26.471333519325185	26.84458951868604	25.06016952716904
46-47	21.685924283957647	26.63834085048488	26.73478965420007	24.940945211357395
48-49	21.643894068360257	26.521469481426852	26.683703659033064	25.150932791179827
50-51	21.68551048529702	26.455037945845277	26.776231052694122	25.08322051616358
52-53	22.032167649360975	26.59963094166759	26.57575856875641	24.792442840215024
54-55	21.873317746956676	26.46130833931971	26.545806285080165	25.119567628643452
56-57	21.704193666530678	26.291657109730664	26.89019172349805	25.113957500240613
58-59	21.757092066828847	26.447704527945366	26.80785690219352	24.98734650303227
60-61	21.688251990424163	26.346195868638294	26.70942029289317	25.256131848044372
62-63	21.82955397611713	26.29023051805202	26.844333504924734	25.035882000906117
64-65	21.972589109259253	26.474813105123797	26.673703881023215	24.878893904593742
66-67	21.811250605637248	26.326913456917122	26.650116760472	25.21171917697363
68-69	21.758921083687767	26.337617731566286	26.81603607979264	25.087425104953308
70-71	21.959780117054077	26.603428582659856	26.663744504532104	24.773046795753963
72-73	21.909334941834167	26.45103160745729	26.595442497492698	25.04419095321585
74-75	21.866317897284066	26.332570806984396	26.789734443748074	25.01137685198347
76-77	21.902532640938137	26.521200462114813	26.658579148716676	24.91768774823037
78-79	21.900938508130768	26.348603487203075	26.603826328527973	25.14663167613818
80-81	21.882970656128776	26.334964835995393	26.81928221552532	24.962782292350514
82-83	21.9948150242076	26.594388105909438	26.503633923831423	24.90716294605154
84-85	22.038606447859856	26.404074054744946	26.5007404347542	25.056579062640992
86-87	22.086150559384564	26.321262764827523	26.668768206868563	24.923818468919347
88-89	22.26091220216344	26.539455439198473	26.538019916601503	24.66161244203658
90-91	22.12034083047182	26.41069219244838	26.464753402497116	25.004213574582685
92-93	22.07004875108007	26.35724052374772	26.51660360672363	25.05610711844858
94-95	22.301142052138584	26.504716127754268	26.368418623611547	24.82572319649561
96-97	22.29609963569839	26.546428733380434	26.145036554930872	25.012435075990304
98-99	22.279832290995103	26.52745162578552	26.39955128052368	24.793164802695696
100-101	22.263350578163436	26.803363523357394	26.199112484800395	24.73417341367877
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	113.0
1	86.5
2	57.0
3	49.0
4	55.5
5	72.0
6	85.0
7	97.5
8	115.5
9	145.5
10	177.5
11	231.5
12	283.5
13	319.5
14	377.5
15	447.5
16	531.0
17	693.5
18	907.5
19	1128.5
20	1463.5
21	2024.5
22	2794.5
23	4067.0
24	6110.0
25	8631.5
26	11975.0
27	17304.5
28	25635.5
29	37053.0
30	52415.0
31	72137.5
32	101102.5
33	139144.0
34	185444.0
35	250068.5
36	333353.0
37	436741.5
38	573786.0
39	750437.5
40	961044.5
41	1193101.0
42	1429277.5
43	1641702.0
44	1817553.5
45	1936259.5
46	1988335.0
47	1974995.5
48	1892306.0
49	1753225.0
50	1575077.0
51	1389980.5
52	1212816.5
53	1047960.0
54	882991.0
55	719921.5
56	584749.0
57	478783.5
58	388866.0
59	310046.0
60	252974.5
61	220235.5
62	189476.5
63	156195.5
64	137311.5
65	124209.5
66	107892.5
67	91647.5
68	70461.5
69	53280.0
70	46082.0
71	39122.0
72	32629.5
73	30217.5
74	26893.0
75	20868.0
76	13452.0
77	4685.5
78	484.0
79	108.0
80	34.0
81	10.0
82	3.0
83	3.0
84	2.0
85	3.5
86	4.0
87	1.0
88	1.0
89	1.5
90	1.0
91	1.5
92	2.0
93	1.0
94	0.5
95	0.5
96	2.0
97	2.5
98	2.0
99	3.5
100	13.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.057981026013614795
2	0.0
3	6.708049518553224E-5
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	5.936623823919603E-4
12-13	0.0
14-15	0.0
16-17	0.0012057719009599419
18-19	0.0020057068060474136
20-21	0.0
22-23	0.0
24-25	1.106828170561282E-4
26-27	0.0
28-29	3.052162530941717E-4
30-31	0.0016518571939437316
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0028089957358941623
40-41	1.6770123796383061E-6
42-43	0.0
44-45	0.0
46-47	0.0022237184154003937
48-49	0.0
50-51	0.0015529134635450714
52-53	4.2596114442812976E-4
54-55	0.0
56-57	6.456497661607478E-4
58-59	1.5260812654708584E-4
60-61	0.0010095614525422601
62-63	0.0029632808748208867
64-65	0.0019185021623062222
66-67	8.569533259951744E-4
68-69	4.0415998349283173E-4
70-71	8.200590536431318E-4
72-73	0.0
74-75	0.0
76-77	8.636613755137276E-4
78-79	1.6770123796383061E-6
80-81	0.0016401181072862635
82-83	0.001079995972487069
84-85	3.354024759276612E-5
86-87	9.558970563938343E-4
88-89	0.0
90-91	0.0019218561870654987
92-93	0.0010213005391997284
94-95	0.0
96-97	2.3478173314936285E-4
98-99	0.0010313626134775581
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2.9814926E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.997586128547184
#Duplication Level	Percentage of deduplicated	Percentage of total
1	62.456893512801905	21.23383616522982
2	16.188383557603775	11.007319285631826
3	6.823414540860699	6.959388706310785
4	3.8372740900396165	5.218322254998575
5	2.259552991817247	3.8409673725661655
6	1.6102326545415795	3.2846414015865912
7	1.1282197614340026	2.6849723957897016
8	0.8852299789481944	2.4076545962290594
9	0.658406304200109	2.0145802531159474
>10	3.7491427571869083	23.221440386559138
>50	0.25963920214777364	6.066444518786018
>100	0.13251525383524304	8.141945999835281
>500	0.007641795595927582	1.7753125760328627
>1k	0.00336016485983194	1.9484913290070183
>5k	9.343412729733745E-5	0.19468275832128853
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.0248297111319344E-5	0.0	0.0	0.0	6.708049518553224E-6
2	6.037244566697901E-5	0.0	6.708049518553224E-6	0.0	1.677012379638306E-5
3	8.720464374119192E-5	1.0062074277829836E-5	6.708049518553224E-6	0.0	2.0124148555659672E-5
4	1.5093111416744753E-4	1.3416099037106447E-5	3.689427235204273E-5	0.0	2.0124148555659672E-5
5	1.7105526272310722E-4	1.3416099037106447E-5	3.689427235204273E-5	0.0	2.0124148555659672E-5
6	2.24719658871533E-4	1.677012379638306E-5	5.7018420907702405E-5	0.0	2.0124148555659672E-5
7	2.582599064642991E-4	2.3478173314936283E-5	6.372647042625563E-5	0.0	2.0124148555659672E-5
8	2.9515417881634183E-4	2.6832198074212895E-5	6.372647042625563E-5	0.0	3.354024759276612E-5
9	3.5552662448332085E-4	3.354024759276612E-5	6.708049518553224E-5	0.0	3.689427235204273E-5
10-11	3.9409790921500194E-4	4.0248297111319344E-5	7.714256946336207E-5	0.0	4.695634662987257E-5
12-13	4.377002310855979E-4	6.372647042625563E-5	7.714256946336207E-5	0.0	6.037244566697901E-5
14-15	5.366439614842579E-4	6.540348280589393E-5	7.714256946336207E-5	1.677012379638306E-6	6.372647042625563E-5
16-17	6.137865309476199E-4	8.2173606602277E-5	7.714256946336207E-5	3.354024759276612E-6	6.708049518553224E-5
18-19	6.775130013738757E-4	8.720464374119192E-5	7.881958184300038E-5	3.354024759276612E-6	6.708049518553224E-5
20-21	7.714256946336208E-4	8.720464374119192E-5	8.049659422263869E-5	3.354024759276612E-6	7.378854470408546E-5
22-23	8.804314993101107E-4	8.888165612083021E-5	8.049659422263869E-5	3.354024759276612E-6	7.378854470408546E-5
24-25	0.0010162695020608135	1.0062074277829836E-4	8.38506189819153E-5	3.354024759276612E-6	7.378854470408546E-5
26-27	0.0011470764676726013	1.0397476753757498E-4	8.552763136155361E-5	3.354024759276612E-6	7.378854470408546E-5
28-29	0.0013114236808771554	1.0397476753757498E-4	1.106828170561282E-4	3.354024759276612E-6	7.714256946336207E-5
30-31	0.0016283790206287952	1.0732879229685158E-4	1.106828170561282E-4	3.354024759276612E-6	7.714256946336207E-5
32-33	0.0019185021623062222	1.1403684181540481E-4	1.106828170561282E-4	3.354024759276612E-6	8.2173606602277E-5
34-35	0.0023595564181510967	1.1739086657468142E-4	1.106828170561282E-4	3.354024759276612E-6	9.558970563938344E-5
36-37	0.0029884360605154613	1.2577592847287295E-4	1.106828170561282E-4	3.354024759276612E-6	1.0229775515793667E-4
38-39	0.0038621595103070184	1.2745294085251125E-4	1.106828170561282E-4	3.354024759276612E-6	1.0565177991721329E-4
40-41	0.005071285436026238	1.2912995323214956E-4	1.106828170561282E-4	3.354024759276612E-6	1.106828170561282E-4
42-43	0.006704695493793947	1.4086903988961771E-4	1.106828170561282E-4	3.354024759276612E-6	1.106828170561282E-4
44-45	0.00876071267123051	1.6267020082491568E-4	1.1403684181540481E-4	3.354024759276612E-6	1.3416099037106448E-4
46-47	0.010955921876177053	1.8111733700093704E-4	1.1403684181540481E-4	3.354024759276612E-6	1.4086903988961771E-4
48-49	0.01396783611000745	1.8111733700093704E-4	1.1403684181540481E-4	3.354024759276612E-6	1.6267020082491568E-4
50-51	0.018177137182899598	1.8447136176021365E-4	1.1403684181540481E-4	3.354024759276612E-6	1.8279434938057535E-4
52-53	0.02391922757078116	1.962104484176818E-4	1.1403684181540481E-4	3.354024759276612E-6	1.9117941127876688E-4
54-55	0.03036063212097189	2.0124148555659673E-4	1.3248397799142618E-4	3.354024759276612E-6	1.9117941127876688E-4
56-57	0.03755166120486095	2.3142770839008623E-4	1.5596215130636245E-4	3.354024759276612E-6	1.945334360380435E-4
58-59	0.0461128094029145	2.3310472076972454E-4	1.8111733700093704E-4	3.354024759276612E-6	1.978874607973201E-4
60-61	0.05727835782654635	2.3645874552900116E-4	1.8111733700093704E-4	3.354024759276612E-6	2.0124148555659673E-4
62-63	0.07241171754040242	2.3813575790863946E-4	1.8111733700093704E-4	3.354024759276612E-6	2.0459551031587334E-4
64-65	0.0907062455898767	2.3813575790863946E-4	1.8447136176021365E-4	3.354024759276612E-6	2.1298057221406485E-4
66-67	0.11216026496258955	2.4316679504755436E-4	1.9117941127876688E-4	3.354024759276612E-6	2.213656341122564E-4
68-69	0.136986756230755	2.515518569457459E-4	1.962104484176818E-4	3.354024759276612E-6	2.3142770839008623E-4
70-71	0.16776496443425687	2.515518569457459E-4	1.978874607973201E-4	3.354024759276612E-6	2.3645874552900116E-4
72-73	0.20757556131449062	2.582599064642991E-4	1.978874607973201E-4	3.354024759276612E-6	2.3813575790863946E-4
74-75	0.2593985978700735	2.8173807977923543E-4	1.9956447317695842E-4	3.354024759276612E-6	2.4148978266791605E-4
76-77	0.32289028656317975	3.0689326547380996E-4	2.0124148555659673E-4	3.354024759276612E-6	2.4316679504755436E-4
78-79	0.3981965274708379	3.119243026127249E-4	2.0124148555659673E-4	3.354024759276612E-6	2.582599064642991E-4
80-81	0.489355566403217	3.136013149923632E-4	2.0124148555659673E-4	3.354024759276612E-6	2.666449683624907E-4
82-83	0.6016114210714458	3.152783273720015E-4	2.0124148555659673E-4	3.354024759276612E-6	2.767070426403205E-4
84-85	0.7448970358001223	3.2701741402946965E-4	2.0124148555659673E-4	3.354024759276612E-6	2.8173807977923543E-4
86-87	0.9261200245809766	3.387565006869378E-4	2.0627252269551165E-4	3.354024759276612E-6	2.8844612929778866E-4
88-89	1.1431203954690345	3.572036368629592E-4	2.1130355983442654E-4	3.354024759276612E-6	3.0186222833489506E-4
90	1.3260371667533235	3.655886987611507E-4	2.1465758459370316E-4	3.354024759276612E-6	3.152783273720015E-4
>>END_MODULE
