##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	SRR19654108_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	35030914
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.368899909377188	32.0	32.0	32.0	32.0	32.0
2	31.444419006595147	32.0	32.0	32.0	32.0	32.0
3	34.96557306497912	37.0	32.0	37.0	32.0	37.0
4	35.86657581928922	37.0	37.0	37.0	32.0	37.0
5	36.04077324388396	37.0	37.0	37.0	37.0	37.0
6	39.39584653714716	41.0	41.0	41.0	37.0	41.0
7	39.612958828308045	41.0	41.0	41.0	37.0	41.0
8	39.716049458486864	41.0	41.0	41.0	37.0	41.0
9	39.65865224070374	41.0	41.0	41.0	37.0	41.0
10-11	39.65640696671517	41.0	41.0	41.0	37.0	41.0
12-13	39.70430570267165	41.0	41.0	41.0	37.0	41.0
14-15	39.664896639579545	41.0	41.0	41.0	37.0	41.0
16-17	39.57724749916602	41.0	41.0	41.0	37.0	41.0
18-19	39.576132512557336	41.0	41.0	41.0	37.0	41.0
20-21	39.63326263768053	41.0	41.0	41.0	37.0	41.0
22-23	39.56198372386173	41.0	41.0	41.0	37.0	41.0
24-25	39.51772729937906	41.0	41.0	41.0	37.0	41.0
26-27	39.46513426683643	41.0	41.0	41.0	37.0	41.0
28-29	39.354192899448755	41.0	41.0	41.0	37.0	41.0
30-31	39.47744704862682	41.0	41.0	41.0	37.0	41.0
32-33	39.54687151468557	41.0	41.0	41.0	37.0	41.0
34-35	39.493359636577	41.0	41.0	41.0	37.0	41.0
36-37	39.33096979713404	41.0	41.0	41.0	37.0	41.0
38-39	39.24943743974251	41.0	41.0	41.0	37.0	41.0
40-41	39.23535968830274	41.0	41.0	41.0	37.0	41.0
42-43	39.2015007658664	41.0	41.0	41.0	37.0	41.0
44-45	39.19637573544327	41.0	41.0	41.0	37.0	41.0
46-47	39.14528252103271	41.0	41.0	41.0	37.0	41.0
48-49	39.11613468035689	41.0	41.0	41.0	37.0	41.0
50-51	39.113994613443424	41.0	41.0	41.0	37.0	41.0
52-53	39.12552558577261	41.0	41.0	41.0	37.0	41.0
54-55	39.127002880941106	41.0	41.0	41.0	37.0	41.0
56-57	39.08917923180651	41.0	41.0	41.0	37.0	41.0
58-59	39.05889318217618	41.0	41.0	41.0	37.0	41.0
60-61	39.01122295867016	41.0	41.0	41.0	37.0	41.0
62-63	38.9512993437739	41.0	41.0	41.0	37.0	41.0
64-65	38.96952590217886	41.0	41.0	41.0	37.0	41.0
66-67	38.965115511973224	41.0	41.0	41.0	37.0	41.0
68-69	38.91314528647469	41.0	41.0	41.0	34.5	41.0
70-71	38.83989084612522	41.0	41.0	41.0	32.0	41.0
72-73	38.85615229736798	41.0	41.0	41.0	32.0	41.0
74-75	38.7557937255077	41.0	41.0	41.0	32.0	41.0
76-77	37.84424215993907	41.0	39.0	41.0	29.5	41.0
78-79	38.39311085345932	41.0	39.0	41.0	32.0	41.0
80-81	38.864181277142805	41.0	41.0	41.0	32.0	41.0
82-83	38.941456822964994	41.0	41.0	41.0	34.5	41.0
84-85	38.91295913660717	41.0	41.0	41.0	32.0	41.0
86-87	38.86456802126259	41.0	41.0	41.0	32.0	41.0
88-89	38.78906155574474	41.0	41.0	41.0	32.0	41.0
90-91	38.67571180129642	41.0	41.0	41.0	32.0	41.0
92-93	38.58895896921217	41.0	41.0	41.0	32.0	41.0
94-95	38.453557220916366	41.0	39.0	41.0	32.0	41.0
96-97	38.485430140361174	41.0	39.0	41.0	32.0	41.0
98-99	38.42761110943323	41.0	37.0	41.0	32.0	41.0
100-101	37.36902922373079	41.0	37.0	41.0	29.5	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	41.0
13	122.0
14	437.0
15	1734.0
16	7515.0
17	20405.0
18	34532.0
19	46274.0
20	57521.0
21	69099.0
22	82926.0
23	98359.0
24	115384.0
25	132923.0
26	154759.0
27	178617.0
28	207800.0
29	240261.0
30	280355.0
31	330991.0
32	390401.0
33	466445.0
34	569497.0
35	716464.0
36	946657.0
37	1345716.0
38	2254721.0
39	5815720.0
40	2.0465238E7
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	37.7759768254842	19.991805778097042	11.969554040179668	30.262663356239088
2	30.44787353729046	22.517050438626228	30.258288298429303	16.77678772565401
3	22.024994285717874	26.05318137572509	29.27230547583092	22.649518862726115
4	24.944824265932862	32.20536080932118	22.31578364415195	20.53403128059401
5	26.633561532387766	33.516920243374265	21.331321961632643	18.51819626260533
6	21.639556489471605	36.49257747032515	21.837835075766808	20.030030964436428
7	21.018692232809	19.306293292832724	38.38072280957328	21.294291664784996
8	22.78091025190144	23.87344358478554	26.211885731783013	27.133760431530007
9	23.079463470464972	23.16447124388476	28.65762794541987	25.0984373402304
10-11	25.54908929866917	29.443820677930354	22.544573059098603	22.462516964301873
12-13	25.638142178591384	23.886945300035414	26.237767240929816	24.237145280443386
14-15	24.344640279725503	26.480449525239337	26.719348515999325	22.45556167903584
16-17	25.248618098467524	26.225284598137026	25.91443293686494	22.611664366530515
18-19	24.84497977985958	26.82405175040537	25.97110369429699	22.35986477543806
20-21	24.932808416227765	26.682839767209433	25.945168938614906	22.439182877947893
22-23	25.009721057080657	26.573492768708206	26.03591470818809	22.380871466023045
24-25	24.699020059601086	26.473504567652345	26.254784846272607	22.572690526473966
26-27	24.76414705174537	26.714088286201477	26.120884592008398	22.40088007004476
28-29	24.9378482217013	26.461816947935013	26.059387064862438	22.540947765501247
30-31	24.672266721254744	26.49072964232183	26.311019467441398	22.52598416898203
32-33	24.712075835540155	26.72420791880231	26.16444402906338	22.399272216594152
34-35	24.987516740214087	26.6341138001709	25.92169447819717	22.45667498141784
36-37	24.690207911789	26.497925518015315	26.329054674394165	22.482811895801518
38-39	24.84631042027247	26.668847236696276	26.09577961642131	22.389062726609943
40-41	24.97845474428672	26.50061171683959	26.018611732482917	22.50232180639078
42-43	24.583233540455296	26.5818924383594	26.387645303901785	22.44722871728352
44-45	24.719994283800347	26.59806304074131	26.274273045078417	22.40766963037992
46-47	25.085578697718248	26.404146920060377	26.05783994103037	22.452434441191
48-49	24.73479147323055	26.430331318022244	26.3466754232498	22.48820178549741
50-51	24.82260075090241	26.53670902806389	26.154602197926422	22.486088023107282
52-53	25.064776050397402	26.516203190265088	26.06291071481484	22.35611004452267
54-55	24.58751850022132	26.49451928713194	26.467208926853854	22.450753285792885
56-57	24.637507902434713	26.625950253098452	26.31750817535946	22.419033669107378
58-59	25.023646405635013	26.39813387653696	26.126113858891863	22.452105858936168
60-61	24.745327798184196	26.518861312039988	26.303983961137867	22.431826928637946
62-63	24.756463006683862	26.571621557215945	26.304373292399134	22.367542143701062
64-65	24.951795434169945	26.52703694799399	26.210114015295176	22.311053602540888
66-67	24.70030460012083	26.544503942767612	26.496769178772077	22.258422278339477
68-69	24.73545330562421	26.709583542571707	26.287318228453096	22.267644923350993
70-71	25.104098120025032	26.577593285377947	26.049408048187868	22.26890054640915
72-73	24.753371727292546	26.543961612359023	26.462096646333926	22.2405700140145
74-75	24.67964290821539	26.810327457361094	26.32555428823909	22.184475346184428
76-77	25.115879122631895	26.60300505619449	26.06830690456118	22.212808916612435
78-79	24.723835106711338	26.697244963098584	26.311095188632017	22.267824741558062
80-81	24.930038165905252	26.666044253997523	26.316571577454575	22.08734600264265
82-83	25.192989227454294	26.50923229027415	26.136997411508734	22.160781070762823
84-85	24.915587678211757	26.675531170106748	26.344481803075343	22.064399348606152
86-87	24.97712020873906	26.910715204290703	26.070857015035347	22.041307571934894
88-89	25.270939262389785	26.70245343869703	26.00070754648309	22.025899752430096
90-91	24.91376331202777	26.77642381811676	26.27211639410836	22.037696475747108
92-93	25.1173948815609	26.89587830908437	26.03623902019799	21.950487789156742
94-95	25.482967304485634	26.863770036565725	25.847761051809957	21.805501607138684
96-97	25.32272576801188	26.740137306862984	26.11976051117928	21.817376413945865
98-99	25.46911637729796	27.017827083182215	25.79340567001494	21.719650869504886
100-101	25.797898206755647	26.832333578441776	25.692886190599445	21.676882024203135
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	57.0
1	47.5
2	36.0
3	36.5
4	39.5
5	54.0
6	74.0
7	80.5
8	92.0
9	121.0
10	154.0
11	181.5
12	223.0
13	280.0
14	345.0
15	435.5
16	530.0
17	641.5
18	785.0
19	1009.0
20	1290.5
21	1580.5
22	2041.5
23	2676.0
24	3558.5
25	4809.0
26	6592.5
27	9079.5
28	12471.0
29	17536.5
30	24789.5
31	35474.5
32	51747.0
33	76659.0
34	115980.0
35	179456.5
36	272889.0
37	403734.0
38	592343.0
39	839422.5
40	1111890.5
41	1395314.5
42	1692654.5
43	1947203.0
44	2144481.0
45	2288608.0
46	2347555.0
47	2343491.5
48	2267310.0
49	2132280.0
50	1950487.0
51	1748385.5
52	1542775.0
53	1335533.5
54	1148739.0
55	966565.0
56	795390.0
57	639878.5
58	502901.5
59	388734.5
60	312414.0
61	263940.5
62	223971.5
63	184813.5
64	148421.5
65	121690.5
66	103062.5
67	86579.5
68	66359.0
69	48384.5
70	36336.0
71	28265.0
72	20656.0
73	13930.5
74	8472.5
75	4629.5
76	2503.5
77	1342.5
78	825.5
79	572.5
80	427.5
81	355.5
82	301.0
83	247.5
84	223.0
85	187.5
86	140.0
87	109.0
88	111.0
89	106.5
90	89.0
91	72.0
92	63.0
93	63.5
94	52.0
95	51.0
96	51.0
97	59.5
98	86.0
99	144.5
100	698.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.017795710383120465
2	0.0066883781565048525
3	7.621839384493365E-4
4	0.0016242796291298593
5	2.6833442027804355E-4
6	0.0011532670828971233
7	0.0
8	0.0238846180262382
9	0.0
10-11	0.0
12-13	0.003875148675823874
14-15	0.0
16-17	0.001081901545589133
18-19	0.0
20-21	3.225722286321162E-4
22-23	4.281932238479418E-6
24-25	2.28369719385569E-5
26-27	0.00537810689153015
28-29	9.691439966425084E-4
30-31	1.8555039700077482E-4
32-33	4.624486817557772E-4
34-35	0.0
36-37	0.0
38-39	9.091969453037964E-4
40-41	0.0
42-43	6.851091581567069E-5
44-45	0.0018469401055307892
46-47	0.0
48-49	0.0014487204073522033
50-51	0.0011318574217047263
52-53	6.280167283103147E-4
54-55	7.193646160645423E-4
56-57	0.00840257836264278
58-59	6.280167283103147E-4
60-61	0.0
62-63	0.004817173768289346
64-65	0.0
66-67	0.0025363311959259757
68-69	2.697617310242034E-4
70-71	6.280167283103147E-5
72-73	1.5985880356989828E-4
74-75	5.723516092100823E-4
76-77	3.468365113168329E-4
78-79	2.1837854416245037E-4
80-81	0.001611433832414421
82-83	0.005199693048260174
84-85	5.338142190637675E-4
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	6.565629432335109E-5
96-97	0.004675870004419525
98-99	2.8546214923196124E-6
100-101	4.595940602634576E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3.5030914E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.7054307497419
#Duplication Level	Percentage of deduplicated	Percentage of total
1	61.93543417764279	18.398187509191654
2	14.725704416212166	8.748667855539178
3	6.923901955938147	6.170324702103694
4	3.9843104739616533	4.734226354789568
5	2.5763017214010464	3.826507618775982
6	1.7743859787369678	3.1625339888810395
7	1.33757394454898	2.781324712772119
8	0.9355825473240333	2.223350605616096
9	0.7530494638483248	2.0132692829529013
>10	4.497514758131002	24.743750183253095
>50	0.3439683096767524	7.016263394125264
>100	0.19240052684738831	10.779238517339774
>500	0.014707052423609004	2.9788503309249927
>1k	0.005124283634533238	2.3531641019725127
>5k	4.0389672479968605E-5	0.07034084176212456
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.28369719385569E-5	0.0	5.709242984639225E-6	0.0	0.0
2	2.569159343087651E-5	1.4273107461598062E-5	5.709242984639225E-6	2.8546214923196124E-6	0.0
3	5.7092429846392247E-5	1.4273107461598062E-5	5.709242984639225E-6	2.8546214923196124E-6	0.0
4	1.3131258864670218E-4	1.4273107461598062E-5	1.4273107461598062E-5	5.709242984639225E-6	0.0
5	1.5129493909293946E-4	1.4273107461598062E-5	2.28369719385569E-5	5.709242984639225E-6	0.0
6	1.9982350446237288E-4	1.4273107461598062E-5	3.140083641551574E-5	5.709242984639225E-6	0.0
7	2.3693358386252781E-4	1.4273107461598062E-5	4.2819322384794185E-5	5.709242984639225E-6	0.0
8	2.9688063520123966E-4	1.4273107461598062E-5	5.423780835407263E-5	5.709242984639225E-6	2.8546214923196124E-6
9	3.1686298564747694E-4	1.9982350446237286E-5	5.423780835407263E-5	8.563864476958838E-6	1.141848596927845E-5
10-11	3.9964700892474575E-4	2.28369719385569E-5	6.137436208487166E-5	8.563864476958838E-6	2.28369719385569E-5
12-13	4.6673061399425664E-4	2.4264282684716703E-5	7.279284805415012E-5	8.563864476958838E-6	2.28369719385569E-5
14-15	5.637877447331234E-4	2.569159343087651E-5	8.278402327726876E-5	1.4273107461598062E-5	2.28369719385569E-5
16-17	6.808272259182275E-4	2.569159343087651E-5	1.384491423775012E-4	1.7127728953917675E-5	2.28369719385569E-5
18-19	8.32122165011167E-4	2.569159343087651E-5	1.5272224983909927E-4	2.1409661192397092E-5	2.28369719385569E-5
20-21	9.777078611194674E-4	2.8546214923196123E-5	1.5985880356989828E-4	2.4264282684716703E-5	2.28369719385569E-5
22-23	0.0011532670828971233	3.8537390146314764E-5	1.8126846476229538E-4	2.7118904177036316E-5	2.28369719385569E-5
24-25	0.001427310746159806	4.424663313095399E-5	1.898323292392542E-4	3.140083641551574E-5	2.28369719385569E-5
26-27	0.0017427464210611233	4.852856536943341E-5	1.9696888297005325E-4	3.568276865399515E-5	2.8546214923196123E-5
28-29	0.0021523846052089878	5.138318686175302E-5	2.1695123341629053E-4	3.996470089247457E-5	2.8546214923196123E-5
30-31	0.0027347273896421885	5.7092429846392247E-5	2.2123316565476995E-4	3.996470089247457E-5	2.8546214923196123E-5
32-33	0.00346408318092985	6.137436208487166E-5	2.2266047640092976E-4	3.996470089247457E-5	2.8546214923196123E-5
34-35	0.00441895407011076	6.422898357719129E-5	2.654797987857239E-4	3.996470089247457E-5	2.8546214923196123E-5
36-37	0.005802018183139612	6.993822656183051E-5	2.6833442027804355E-4	4.139201163863438E-5	3.425545790783535E-5
38-39	0.007788834741794062	7.136553730799031E-5	2.783255955011622E-4	4.424663313095399E-5	3.425545790783535E-5
40-41	0.01060491884396736	7.422015880030993E-5	3.0544449967819854E-4	4.56739438771138E-5	3.568276865399515E-5
42-43	0.014227433517720947	7.992940178494914E-5	3.368453360937143E-4	4.56739438771138E-5	3.996470089247457E-5
44-45	0.018586440536493	8.278402327726876E-5	3.3969995758603385E-4	4.8528565369433405E-5	4.56739438771138E-5
46-47	0.023413605480005463	8.563864476958837E-5	3.511184435553123E-4	5.2810497607912826E-5	5.423780835407263E-5
48-49	0.029572451349685024	8.849326626190798E-5	3.5682768653995156E-4	5.851974059255205E-5	5.423780835407263E-5
50-51	0.038313302359167675	9.562981999270702E-5	3.611096187784309E-4	6.565629432335109E-5	5.423780835407263E-5
52-53	0.04959476649681478	9.848444148502662E-5	3.75382726240029E-4	6.565629432335109E-5	5.566511910023244E-5
54-55	0.06208516283645925	1.0419368446966585E-4	3.982196981785859E-4	6.85109158156707E-5	5.7092429846392247E-5
56-57	0.07597432370734032	1.1133023820046488E-4	4.367570883249007E-4	7.136553730799031E-5	5.7092429846392247E-5
58-59	0.09189169314851448	1.1133023820046488E-4	4.595940602634576E-4	7.136553730799031E-5	5.7092429846392247E-5
60-61	0.1125263246057468	1.1418485969278449E-4	4.810037214558547E-4	7.136553730799031E-5	5.7092429846392247E-5
62-63	0.13930124689295859	1.170394811851041E-4	5.323869083176077E-4	7.136553730799031E-5	6.565629432335109E-5
64-65	0.17229781672268102	1.1846679193126391E-4	5.537965695100048E-4	7.422015880030992E-5	7.707478029262953E-5
66-67	0.2106239648785641	1.3416721013902178E-4	5.580785017484842E-4	7.422015880030992E-5	7.707478029262953E-5
68-69	0.25419262540509224	1.4558569610830023E-4	5.709242984639225E-4	7.422015880030992E-5	7.707478029262953E-5
70-71	0.3072486204613445	1.5129493909293946E-4	5.737789199562421E-4	7.422015880030992E-5	7.992940178494914E-5
72-73	0.37519717584302825	1.5414956058525906E-4	5.909066489101597E-4	7.422015880030992E-5	9.420250924654721E-5
74-75	0.4608072743976934	1.5700418207757868E-4	5.909066489101597E-4	7.564746954646972E-5	9.705713073886682E-5
76-77	0.5659715301747479	1.627134250622179E-4	5.909066489101597E-4	7.707478029262953E-5	9.705713073886682E-5
78-79	0.6922871038991447	1.798411540161356E-4	5.923339596563195E-4	7.707478029262953E-5	9.705713073886682E-5
80-81	0.8397297312881988	1.855503970007748E-4	5.96615891894799E-4	7.707478029262953E-5	9.705713073886682E-5
82-83	1.019192647956602	1.9696888297005325E-4	6.080343778640774E-4	7.707478029262953E-5	9.991175223118644E-5
84-85	1.242274181027649	1.9696888297005325E-4	6.108889993563971E-4	7.707478029262953E-5	1.0419368446966585E-4
86-87	1.5184488192343482	1.9696888297005325E-4	6.251621068179951E-4	7.850209103878934E-5	1.0562099521582565E-4
88-89	1.8449047604067652	2.1409661192397093E-4	6.322986605487942E-4	7.992940178494914E-5	1.0562099521582565E-4
90	2.1208467469618406	2.4835206983180627E-4	6.394352142795931E-4	7.992940178494914E-5	1.0562099521582565E-4
>>END_MODULE
