##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	SRR19654136_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	30386663
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.62080140224677	32.0	32.0	32.0	32.0	32.0
2	31.68944434602773	32.0	32.0	32.0	32.0	32.0
3	35.74435949087269	37.0	37.0	37.0	32.0	37.0
4	36.357691728111114	37.0	37.0	37.0	37.0	37.0
5	36.435668339099955	37.0	37.0	37.0	37.0	37.0
6	40.09198519100304	41.0	41.0	41.0	37.0	41.0
7	40.10654957406807	41.0	41.0	41.0	37.0	41.0
8	40.24494789046102	41.0	41.0	41.0	37.0	41.0
9	40.2204412837303	41.0	41.0	41.0	41.0	41.0
10-11	40.285159841342235	41.0	41.0	41.0	41.0	41.0
12-13	40.24544216980983	41.0	41.0	41.0	41.0	41.0
14-15	40.277009801964766	41.0	41.0	41.0	41.0	41.0
16-17	40.27812616344217	41.0	41.0	41.0	41.0	41.0
18-19	40.274593890089214	41.0	41.0	41.0	41.0	41.0
20-21	40.20692640715435	41.0	41.0	41.0	41.0	41.0
22-23	40.16607376400627	41.0	41.0	41.0	39.0	41.0
24-25	40.20315812565532	41.0	41.0	41.0	41.0	41.0
26-27	40.17730193012638	41.0	41.0	41.0	41.0	41.0
28-29	40.09513881139236	41.0	41.0	41.0	39.0	41.0
30-31	40.11103889558389	41.0	41.0	41.0	37.0	41.0
32-33	40.117350496828166	41.0	41.0	41.0	39.0	41.0
34-35	40.115501313849435	41.0	41.0	41.0	39.0	41.0
36-37	40.09779464760577	41.0	41.0	41.0	37.0	41.0
38-39	40.07503096012879	41.0	41.0	41.0	37.0	41.0
40-41	40.062482889286	41.0	41.0	41.0	37.0	41.0
42-43	40.011782076893404	41.0	41.0	41.0	37.0	41.0
44-45	40.02777169049461	41.0	41.0	41.0	37.0	41.0
46-47	40.01388472633536	41.0	41.0	41.0	37.0	41.0
48-49	39.99035464999892	41.0	41.0	41.0	37.0	41.0
50-51	39.92419067207216	41.0	41.0	41.0	37.0	41.0
52-53	39.94410588948185	41.0	41.0	41.0	37.0	41.0
54-55	39.890720132710854	41.0	41.0	41.0	37.0	41.0
56-57	39.88925667816831	41.0	41.0	41.0	37.0	41.0
58-59	39.88139442952324	41.0	41.0	41.0	37.0	41.0
60-61	39.8113154774514	41.0	41.0	41.0	37.0	41.0
62-63	39.76933355597487	41.0	41.0	41.0	37.0	41.0
64-65	39.76023627536857	41.0	41.0	41.0	37.0	41.0
66-67	39.7278054355623	41.0	41.0	41.0	37.0	41.0
68-69	39.64529323275807	41.0	41.0	41.0	37.0	41.0
70-71	39.66563919835488	41.0	41.0	41.0	37.0	41.0
72-73	39.580546208710054	41.0	41.0	41.0	37.0	41.0
74-75	39.61896786758059	41.0	41.0	41.0	37.0	41.0
76-77	39.059863154437195	41.0	39.0	41.0	34.5	41.0
78-79	39.38488403613125	41.0	41.0	41.0	37.0	41.0
80-81	39.6004584972032	41.0	41.0	41.0	37.0	41.0
82-83	39.74213562048587	41.0	41.0	41.0	37.0	41.0
84-85	39.701440678102756	41.0	41.0	41.0	37.0	41.0
86-87	39.719375355563066	41.0	41.0	41.0	37.0	41.0
88-89	39.655766051046804	41.0	41.0	41.0	37.0	41.0
90-91	39.67246069435133	41.0	41.0	41.0	37.0	41.0
92-93	39.62607726949155	41.0	41.0	41.0	37.0	41.0
94-95	39.48380511542186	41.0	41.0	41.0	37.0	41.0
96-97	39.44559552327283	41.0	41.0	41.0	37.0	41.0
98-99	39.49693301301298	41.0	41.0	41.0	37.0	41.0
100-101	38.955968528034816	41.0	39.0	41.0	34.5	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	3.0
15	5.0
16	6.0
17	17.0
18	50.0
19	176.0
20	471.0
21	1529.0
22	3725.0
23	7830.0
24	14832.0
25	25901.0
26	42831.0
27	66476.0
28	97023.0
29	133917.0
30	175745.0
31	222548.0
32	274049.0
33	331943.0
34	400282.0
35	491329.0
36	622346.0
37	845083.0
38	1338271.0
39	3040122.0
40	2.2250152E7
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.868260807073256	11.999807875131212	5.701967186152208	42.42996413164333
2	24.351936900738327	13.688110471360412	31.792072726116714	30.167879901784545
3	20.554122708373736	16.212829951087425	21.932859162587217	41.300188177951625
4	23.746691346918663	23.906717995534315	21.296111510763545	31.050479146783477
5	24.64142574655203	28.561125649104675	23.413169784388632	23.384278819954663
6	20.348884640606965	31.834999453543155	24.880619500732937	22.935496405116943
7	15.857447064852103	25.448283018112257	40.07649671831356	18.617773198722084
8	18.45839735676142	24.583492435480657	31.511824776547527	25.4462854312104
9	18.55844872771796	22.82741731218213	34.02937241001502	24.584761550084885
10-11	21.25318386782162	31.36811164365495	24.840892587131307	22.537811901392125
12-13	21.65361672557919	25.346088156439023	27.52730705211856	25.472988065863223
14-15	21.115823083304672	26.18506678406905	27.460264392967403	25.238845739658878
16-17	21.662707633675222	26.57948155357631	26.821894130102898	24.93591668264557
18-19	21.45521588661019	26.851337919953387	26.639402716574907	25.054043476861516
20-21	21.62104739900201	26.767324663216623	26.767499082447888	24.84412885533348
22-23	21.634325230118225	26.941727691520455	26.70162728957767	24.72231978878365
24-25	21.61087450785357	26.651228972579666	26.538001664381532	25.199894855185228
26-27	21.399437641441576	26.783776816822563	26.774290747226836	25.042494794509025
28-29	21.44558538765282	27.00802180803129	26.64657305315482	24.89981975116107
30-31	21.56754593289826	26.687046879744575	26.661731497137414	25.083675690219753
32-33	21.626166157746322	26.639962205035577	26.753247710623608	24.980623926594493
34-35	21.770798985884362	26.793319613523458	26.47860065245145	24.957280748140732
36-37	21.582193082429676	26.66009259590028	26.437066293523507	25.32064802814653
38-39	21.603365391814563	26.670575780011767	26.69994514142924	25.026113686744427
40-41	21.731646453919563	26.98387217290861	26.532519232176416	24.751962140995413
42-43	21.761035425311427	26.64123237224173	26.530335035472635	25.06739716697421
44-45	21.750450607484396	26.607818037591713	26.668425046603407	24.97330630832049
46-47	21.74493133166849	26.7366455262665	26.544699110928992	24.973724031136022
48-49	21.61373369823645	26.56744951288093	26.547478481654014	25.271338307228604
50-51	21.617353789594603	26.570733980779853	26.720834416319743	25.0910778133058
52-53	21.99076450086013	26.732803796191767	26.379755815898577	24.896675887049525
54-55	21.841041183154598	26.59914137553512	26.37010299922901	25.189714442081268
56-57	21.731630912580517	26.466119978943993	26.74319630016856	25.05905280830693
58-59	21.809664831078102	26.58771072187827	26.667828698190004	24.934795748853624
60-61	21.803730402326128	26.44863818779318	26.578998100546947	25.168633309333753
62-63	21.774445874919067	26.425779793829008	26.712503468020177	25.087270863231748
64-65	21.91550229303999	26.599170061119487	26.541399983166574	24.943927662673953
66-67	21.813826232184738	26.417732000351414	26.527663994328186	25.240777773135665
68-69	21.820643635129436	26.448290119203115	26.731418815374013	24.999647430293432
70-71	22.045403610292375	26.686396572590105	26.520574064762094	24.74762575235543
72-73	21.999444226492972	26.478793413346775	26.416048401000985	25.10571395915927
74-75	21.93713594199596	26.41485810479447	26.677682157925258	24.97032379528431
76-77	21.939648325319567	26.59020176055528	26.57233701509113	24.897812899034026
78-79	21.856466447433913	26.43033079902028	26.532080684453753	25.181122069092048
80-81	21.946577984382326	26.423392346849724	26.650268547019927	24.979761121748023
82-83	22.050862164705613	26.673344767856534	26.430363755373172	24.845429312064688
84-85	22.084553072521825	26.47493951202666	26.372524366728655	25.06798304872286
86-87	22.06030553725573	26.413834006918513	26.581077402660647	24.944783053165114
88-89	22.219637932892436	26.508276039858288	26.513036361137353	24.75904966611192
90-91	22.084746718215005	26.35997510562355	26.51578380936813	25.039494366793313
92-93	22.053752845983354	26.323038459343024	26.516224797517168	25.10698389715645
94-95	22.262742382317054	26.482854967716367	26.356946008313404	24.897456641653175
96-97	22.217258734036598	26.442220173530956	26.19919318013213	25.14132791230032
98-99	22.214846701359313	26.49982741544455	26.436095280233236	24.849230602962898
100-101	22.269433279806925	26.683271734683778	26.304218608658598	24.743076376850695
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	57.0
1	40.0
2	20.5
3	17.0
4	22.5
5	34.5
6	49.5
7	56.5
8	73.5
9	99.0
10	123.0
11	143.0
12	167.5
13	194.0
14	215.5
15	291.0
16	349.0
17	394.0
18	513.5
19	677.0
20	959.5
21	1338.0
22	1913.5
23	2796.5
24	4234.5
25	6526.5
26	9617.5
27	14333.5
28	21005.5
29	30736.0
30	44553.0
31	62404.5
32	87250.5
33	120390.0
34	163835.0
35	225548.0
36	306981.5
37	414223.5
38	560180.0
39	749885.5
40	962487.5
41	1183354.5
42	1412212.0
43	1624620.0
44	1801856.0
45	1920728.0
46	1978073.0
47	1973930.5
48	1895286.0
49	1772461.5
50	1628588.5
51	1456943.0
52	1298991.0
53	1152121.5
54	994028.0
55	846097.0
56	706619.5
57	572013.5
58	451450.5
59	358084.0
60	289553.5
61	244574.0
62	208799.0
63	173104.5
64	143229.0
65	120151.5
66	102017.0
67	82352.0
68	58335.5
69	39783.5
70	29242.5
71	21623.0
72	16401.0
73	13371.0
74	10204.0
75	6420.5
76	3618.5
77	1384.0
78	210.5
79	64.5
80	19.5
81	10.5
82	8.0
83	5.0
84	3.5
85	5.0
86	3.5
87	0.0
88	0.0
89	0.5
90	0.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.034834361377555675
2	0.0
3	0.0
4	0.0200680147076367
5	0.0
6	0.0
7	0.0
8	0.0
9	0.00285980727794954
10-11	0.00348179067902257
12-13	0.00168001336639038
14-15	0.0
16-17	8.046293204357451E-4
18-19	5.5945596921912744E-5
20-21	3.471917926624585E-4
22-23	0.0
24-25	1.6454587329974338E-6
26-27	0.0
28-29	1.958095892266946E-4
30-31	0.0
32-33	0.001989359608193897
34-35	6.170470248740376E-4
36-37	3.702282149244226E-4
38-39	0.0041333923372895535
40-41	7.075472551888966E-5
42-43	0.0
44-45	0.0024303425486372097
46-47	1.8922775429470489E-4
48-49	4.9363761989923E-4
50-51	2.9618257193953804E-5
52-53	0.0
54-55	8.901931745516116E-4
56-57	2.9453711320654065E-4
58-59	7.371655123828504E-4
60-61	6.910926678589222E-5
62-63	2.9453711320654065E-4
64-65	0.002272378510269456
66-67	0.001989359608193897
68-69	0.008558030870319653
70-71	0.0087851041754733
72-73	6.055288137430556E-4
74-75	0.0012011848750881266
76-77	0.0
78-79	0.0012768759768060087
80-81	0.008857504359725186
82-83	0.005852896713271872
84-85	1.4480036850377418E-4
86-87	0.010206780520783082
88-89	1.9251867176069975E-4
90-91	0.0022509875467404896
92-93	6.499561995339864E-4
94-95	0.014457000428115454
96-97	0.0010744845526473243
98-99	3.5706454506044316E-4
100-101	0.015710839982659496
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3.0386663E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.01091746890358
#Duplication Level	Percentage of deduplicated	Percentage of total
1	63.6864113382926	21.660332799173148
2	15.414448753177645	10.485190887459371
3	6.462408341221935	6.5937731023096
4	3.6538850139389916	4.970879265997706
5	2.2466612584844436	3.820550532144874
6	1.5625402264479455	3.188605601013779
7	1.1400201108052594	2.71411909310416
8	0.8300937535587807	2.2585800114992063
9	0.6587697536341214	2.016482734967404
>10	3.9353132855849995	24.381410335421595
>50	0.26910565811189835	6.314753167420068
>100	0.12940756103696296	8.017815194658628
>500	0.0076671826053671495	1.7607403090155964
>1k	0.003237307174108748	1.752859463862354
>5k	3.045592496081599E-5	0.06390750195253016
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.6200092125943544E-5	0.0	0.0	3.2909174659948676E-6	0.0
2	5.265467945591788E-5	3.2909174659948676E-6	0.0	3.2909174659948676E-6	3.2909174659948676E-6
3	8.227293664987169E-5	3.2909174659948676E-6	0.0	3.2909174659948676E-6	1.9745504795969204E-5
4	1.6125495583374852E-4	3.2909174659948676E-6	3.2909174659948676E-6	3.2909174659948676E-6	1.9745504795969204E-5
5	1.8100046062971773E-4	3.2909174659948676E-6	3.2909174659948676E-6	3.2909174659948676E-6	1.9745504795969204E-5
6	2.632733972795894E-4	3.2909174659948676E-6	1.6454587329974337E-5	3.2909174659948676E-6	1.9745504795969204E-5
7	2.9947348940553294E-4	9.872752397984602E-6	2.3036422261964074E-5	3.2909174659948676E-6	2.3036422261964074E-5
8	3.422554164634662E-4	1.9745504795969204E-5	2.3036422261964074E-5	3.2909174659948676E-6	2.3036422261964074E-5
9	3.850373435213995E-4	1.9745504795969204E-5	2.3036422261964074E-5	3.2909174659948676E-6	2.3036422261964074E-5
10-11	4.344011055113225E-4	2.3036422261964074E-5	2.7972798460956372E-5	3.2909174659948676E-6	2.7972798460956372E-5
12-13	4.903467024332353E-4	2.632733972795894E-5	2.9618257193953807E-5	3.2909174659948676E-6	2.9618257193953807E-5
14-15	5.923651438790762E-4	2.632733972795894E-5	2.9618257193953807E-5	3.2909174659948676E-6	2.9618257193953807E-5
16-17	6.351470709370095E-4	3.126371592695124E-5	2.9618257193953807E-5	3.2909174659948676E-6	3.126371592695124E-5
18-19	7.174200075868811E-4	3.6200092125943544E-5	2.9618257193953807E-5	3.2909174659948676E-6	3.2909174659948674E-5
20-21	8.079202379017399E-4	3.6200092125943544E-5	2.9618257193953807E-5	3.2909174659948676E-6	3.6200092125943544E-5
22-23	8.91838633284609E-4	3.6200092125943544E-5	2.9618257193953807E-5	3.2909174659948676E-6	3.6200092125943544E-5
24-25	0.0010037298271284346	3.949100959193841E-5	2.9618257193953807E-5	3.2909174659948676E-6	3.6200092125943544E-5
26-27	0.0011666302416951805	5.594559692191275E-5	2.9618257193953807E-5	3.2909174659948676E-6	3.6200092125943544E-5
28-29	0.0013558579959898854	5.594559692191275E-5	3.2909174659948674E-5	3.2909174659948676E-6	3.6200092125943544E-5
30-31	0.0016717860727253927	5.594559692191275E-5	3.2909174659948674E-5	3.2909174659948676E-6	3.6200092125943544E-5
32-33	0.0021571963989596354	5.759105565491018E-5	3.2909174659948674E-5	3.2909174659948676E-6	3.6200092125943544E-5
34-35	0.002719943285644758	6.252743185390248E-5	3.2909174659948674E-5	3.2909174659948676E-6	3.6200092125943544E-5
36-37	0.003516345312415516	6.252743185390248E-5	3.2909174659948674E-5	3.2909174659948676E-6	3.6200092125943544E-5
38-39	0.0047010756001736684	6.252743185390248E-5	3.2909174659948674E-5	3.2909174659948676E-6	3.7845550858940976E-5
40-41	0.006305397864846166	6.252743185390248E-5	3.2909174659948674E-5	3.2909174659948676E-6	4.278192705793328E-5
42-43	0.008447785135208826	8.227293664987169E-5	3.6200092125943544E-5	3.2909174659948676E-6	4.771830325692558E-5
44-45	0.010892936812443011	1.0530935891183576E-4	3.7845550858940976E-5	3.2909174659948676E-6	4.936376198992301E-5
46-47	0.013614525556820765	1.2505486370780497E-4	3.949100959193841E-5	3.2909174659948676E-6	5.100922072292045E-5
48-49	0.01732997137592897	1.2505486370780497E-4	3.949100959193841E-5	3.2909174659948676E-6	5.265467945591788E-5
50-51	0.022259765739989286	1.316366986397947E-4	3.949100959193841E-5	3.2909174659948676E-6	5.265467945591788E-5
52-53	0.02806658960873723	1.316366986397947E-4	3.949100959193841E-5	3.2909174659948676E-6	5.594559692191275E-5
54-55	0.034610578989868024	1.36573074838787E-4	4.278192705793328E-5	3.2909174659948676E-6	5.759105565491018E-5
56-57	0.041695924294154973	1.5796403836775363E-4	4.607284452392815E-5	3.2909174659948676E-6	6.417289058689992E-5
58-59	0.05037901002818243	1.5796403836775363E-4	5.9236514387907614E-5	3.2909174659948676E-6	7.240018425188709E-5
60-61	0.06166027510161283	1.6125495583374852E-4	5.9236514387907614E-5	3.2909174659948676E-6	7.569110171788195E-5
62-63	0.07516455492332277	1.6125495583374852E-4	5.9236514387907614E-5	3.2909174659948676E-6	7.569110171788195E-5
64-65	0.09174748803447091	1.6125495583374852E-4	5.9236514387907614E-5	3.2909174659948676E-6	9.050023031485885E-5
66-67	0.11081835474991117	1.6125495583374852E-4	5.9236514387907614E-5	3.2909174659948676E-6	9.708206524684859E-5
68-69	0.1334253122825629	1.6125495583374852E-4	5.9236514387907614E-5	3.2909174659948676E-6	1.020184414458409E-4
70-71	0.16059677234054953	1.6125495583374852E-4	5.9236514387907614E-5	3.2909174659948676E-6	1.0530935891183576E-4
72-73	0.1946692863247274	1.6454587329974338E-4	5.9236514387907614E-5	3.2909174659948676E-6	1.118911938438255E-4
74-75	0.23674860250367077	1.7441862569772797E-4	5.9236514387907614E-5	3.2909174659948676E-6	1.118911938438255E-4
76-77	0.2879816056142789	1.9910050669268948E-4	5.9236514387907614E-5	3.2909174659948676E-6	1.118911938438255E-4
78-79	0.34817577698479096	2.0239142415868437E-4	5.9236514387907614E-5	3.2909174659948676E-6	1.118911938438255E-4
80-81	0.42133616317132294	2.040368828916818E-4	5.9236514387907614E-5	3.2909174659948676E-6	1.118911938438255E-4
82-83	0.5107915271907284	2.0568234162467923E-4	5.9236514387907614E-5	3.2909174659948676E-6	1.1518211130982037E-4
84-85	0.6204119879830174	2.0732780035767666E-4	5.9236514387907614E-5	4.936376198992302E-6	1.168275700428178E-4
86-87	0.7558348871674392	2.0732780035767666E-4	6.252743185390248E-5	6.581834931989735E-6	1.1847302877581523E-4
88-89	0.9166801237766713	2.2378238768765098E-4	6.581834931989735E-5	6.581834931989735E-6	1.267003224408024E-4
90	1.0550121940010326	2.3036422261964073E-4	6.581834931989735E-5	9.872752397984602E-6	1.3492761610578956E-4
>>END_MODULE
