##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	SRR19654128_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	30717334
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.281792586557152	32.0	32.0	32.0	32.0	32.0
2	31.260877913428295	32.0	32.0	32.0	32.0	32.0
3	34.88745517433251	37.0	32.0	37.0	32.0	37.0
4	35.663785730884065	37.0	37.0	37.0	32.0	37.0
5	35.93259248996023	37.0	37.0	37.0	37.0	37.0
6	39.25186830992559	41.0	41.0	41.0	37.0	41.0
7	39.37059251300911	41.0	41.0	41.0	37.0	41.0
8	39.43238068772505	41.0	41.0	41.0	37.0	41.0
9	39.401460361110765	41.0	41.0	41.0	37.0	41.0
10-11	39.475799543671336	41.0	41.0	41.0	37.0	41.0
12-13	39.480911624687224	41.0	41.0	41.0	37.0	41.0
14-15	39.460574963960084	41.0	41.0	41.0	37.0	41.0
16-17	39.29010258507461	41.0	41.0	41.0	37.0	41.0
18-19	39.1175514613345	41.0	41.0	41.0	37.0	41.0
20-21	38.98147197605105	41.0	41.0	41.0	37.0	41.0
22-23	38.82447146943156	41.0	41.0	41.0	34.5	41.0
24-25	38.80196780749267	41.0	41.0	41.0	34.5	41.0
26-27	38.760747417077276	41.0	41.0	41.0	32.0	41.0
28-29	38.92068579258864	41.0	41.0	41.0	37.0	41.0
30-31	39.03680322647792	41.0	41.0	41.0	37.0	41.0
32-33	39.09883756513504	41.0	41.0	41.0	37.0	41.0
34-35	39.07120417741982	41.0	41.0	41.0	37.0	41.0
36-37	39.0293687433942	41.0	41.0	41.0	37.0	41.0
38-39	39.10228023043927	41.0	41.0	41.0	37.0	41.0
40-41	39.074511088755294	41.0	41.0	41.0	37.0	41.0
42-43	38.963151896580605	41.0	41.0	41.0	37.0	41.0
44-45	38.97975239322527	41.0	41.0	41.0	37.0	41.0
46-47	38.945172667002936	41.0	41.0	41.0	34.5	41.0
48-49	38.97184915201299	41.0	41.0	41.0	34.5	41.0
50-51	38.87425355664004	41.0	41.0	41.0	32.0	41.0
52-53	38.937480397875674	41.0	41.0	41.0	34.5	41.0
54-55	38.80433928608518	41.0	41.0	41.0	32.0	41.0
56-57	38.78103148534961	41.0	41.0	41.0	32.0	41.0
58-59	38.75956459958407	41.0	41.0	41.0	32.0	41.0
60-61	38.76724695574167	41.0	41.0	41.0	32.0	41.0
62-63	38.77111835291436	41.0	41.0	41.0	32.0	41.0
64-65	38.7533241328821	41.0	41.0	41.0	32.0	41.0
66-67	38.696958547900024	41.0	41.0	41.0	32.0	41.0
68-69	38.64063401465765	41.0	41.0	41.0	32.0	41.0
70-71	38.58454757173914	41.0	41.0	41.0	32.0	41.0
72-73	38.53699733512029	41.0	41.0	41.0	32.0	41.0
74-75	38.503441379385336	41.0	41.0	41.0	32.0	41.0
76-77	37.802021506814356	41.0	39.0	41.0	32.0	41.0
78-79	38.35849943878593	41.0	39.0	41.0	32.0	41.0
80-81	38.74410137611552	41.0	41.0	41.0	32.0	41.0
82-83	38.76712778849883	41.0	41.0	41.0	32.0	41.0
84-85	38.63813339074283	41.0	41.0	41.0	32.0	41.0
86-87	38.67493380122116	41.0	41.0	41.0	32.0	41.0
88-89	38.547429018416764	41.0	41.0	41.0	32.0	41.0
90-91	38.4445494358332	41.0	39.0	41.0	32.0	41.0
92-93	38.41291153392414	41.0	37.0	41.0	32.0	41.0
94-95	38.23038942767624	41.0	37.0	41.0	32.0	41.0
96-97	38.19148151008157	41.0	37.0	41.0	32.0	41.0
98-99	38.09953840720682	41.0	37.0	41.0	32.0	41.0
100-101	36.99325774821474	41.0	37.0	41.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	55.0
13	209.0
14	687.0
15	1515.0
16	4456.0
17	11741.0
18	22362.0
19	34718.0
20	47760.0
21	61589.0
22	77288.0
23	95039.0
24	114668.0
25	137116.0
26	161231.0
27	188891.0
28	221730.0
29	259215.0
30	305578.0
31	359805.0
32	424411.0
33	507523.0
34	622827.0
35	789306.0
36	1028534.0
37	1419849.0
38	2292092.0
39	5366112.0
40	1.6161027E7
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	28.98950698936615	20.159786986281013	12.508321255365088	38.342384768987756
2	27.464782427353505	24.910273892080063	31.189504528653835	16.435439151912597
3	20.913809615801952	27.99255808235841	28.288926709651598	22.804705592188043
4	24.8127789125168	30.633415516139394	22.933713592535646	21.62009197880816
5	28.263449626611937	32.11091424520462	21.75788827599873	17.867747852184714
6	22.67762032957157	37.16716092755297	21.615361754009818	18.539856988865644
7	22.420177378935932	21.948947941261416	35.174564588420765	20.45631009138189
8	22.833733302602948	24.234898220358417	27.60285446166157	25.328514015377063
9	23.9942469751383	22.865229845082897	29.484564953214026	23.65595822656478
10-11	25.146651011086398	30.12195122943811	23.065906536478785	21.665491222996707
12-13	25.87025574150686	25.028004581267073	25.50731408933985	23.594425587886217
14-15	25.04605029801302	26.761569900822295	25.522995830619294	22.66938397054539
16-17	26.080247373268055	26.229860110284093	24.86538166133685	22.824510855111
18-19	25.571473865642957	26.885847965508823	24.909617855717936	22.633060313130283
20-21	25.864262712013257	26.915764703185875	24.783693909547488	22.43627867525338
22-23	25.668260076198905	26.661826862990836	25.037991118565223	22.631921942245032
24-25	25.13046063562409	26.842941910104056	25.40075148386636	22.625845970405486
26-27	25.495510184732872	26.882751818983692	25.243037436116296	22.378700560167143
28-29	25.605375039192683	26.519302561775817	25.109984095352804	22.765338303678696
30-31	25.26492762907506	26.59020351095802	25.369757348055387	22.775111511911525
32-33	25.497747177713126	26.8210348755265	25.12894711088084	22.552270835879533
34-35	25.67896493779186	26.59974895684913	24.97909465915545	22.742191446203563
36-37	25.335668640354026	26.624430714379365	25.35152266881887	22.68837797644774
38-39	25.610076240748036	26.81591773017475	25.105668712401087	22.468337316676127
40-41	25.797077309401434	26.427910181934966	24.997144690791824	22.777867817871776
42-43	25.256992972783408	26.63206699133735	25.418657601752294	22.692282434126945
44-45	25.51034818156475	26.67055239816132	25.28006609162073	22.5390333286532
46-47	25.709780668978205	26.468698534622202	25.118789734535422	22.702731061864164
48-49	25.311681289485417	26.50918176707291	25.437311829103525	22.74182511433815
50-51	25.613223863126382	26.590108129677475	25.146007232087065	22.650660775109078
52-53	25.820951090816948	26.423151215599134	25.047286562092875	22.708611131491047
54-55	25.307142626211608	26.54354339796011	25.41955009598019	22.729763879848086
56-57	25.477049015771957	26.711558994311407	25.283038636709133	22.528353353207507
58-59	25.732527700453602	26.356296199301187	25.13127873095729	22.77989736928792
60-61	25.42005398879741	26.498622119356963	25.31939655021563	22.76192734163
62-63	25.553673119414793	26.56427103284045	25.292344626262537	22.589711221482222
64-65	25.668395253165137	26.376214496982108	25.26804040591356	22.68734984393919
66-67	25.3245974101358	26.601668803944616	25.467559565217808	22.60617422070177
68-69	25.63603037754694	26.764489225363697	25.197330523115465	22.402149873973894
70-71	25.896604139094688	26.462904084075117	24.997053191804714	22.64343858502548
72-73	25.401420013525723	26.568594199957822	25.46642454176919	22.563561244747266
74-75	25.454306114683273	26.777641401678302	25.28231692186963	22.485735561768795
76-77	25.770570202725096	26.423558274906227	25.191683542870873	22.614187979497803
78-79	25.34580589040142	26.606200766473787	25.52685162859686	22.521141714527936
80-81	25.703232903002426	26.6813289650935	25.25278360370541	22.362654528198668
82-83	25.858694803563136	26.353746726128286	25.216720328546533	22.57083814176204
84-85	25.542781400216825	26.569629886059882	25.503945133072538	22.38364358065076
86-87	25.670173800535558	26.760241240283882	25.264281919813758	22.305303039366805
88-89	25.778379632746013	26.534976661117405	25.174491590684827	22.51215211545175
90-91	25.403751908701693	26.7006362189764	25.48622458825313	22.409387284068774
92-93	25.775014176855333	26.78454106921929	25.224093943720987	22.216350810204393
94-95	26.055131801502434	26.512660178356494	25.109903296884834	22.32230472325623
96-97	25.591817741110106	26.62784561388567	25.453499244556564	22.32683740044766
98-99	25.83962009401018	26.891058392984224	25.185086571033803	22.084234941971793
100-101	26.135082156328277	26.547368393977912	25.134373022241373	22.183176427452437
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	64.0
1	42.0
2	19.5
3	19.0
4	28.0
5	38.0
6	40.0
7	54.5
8	73.5
9	88.0
10	111.0
11	139.5
12	173.0
13	219.5
14	280.5
15	334.0
16	410.0
17	533.5
18	719.0
19	917.5
20	1148.5
21	1529.5
22	2040.0
23	2694.0
24	3552.5
25	4916.0
26	6739.0
27	9158.5
28	12531.5
29	16902.0
30	22942.0
31	31623.0
32	44127.5
33	62495.0
34	90715.5
35	134496.5
36	198931.5
37	290534.5
38	426417.5
39	614278.5
40	837909.5
41	1087116.0
42	1364502.5
43	1624995.5
44	1836219.5
45	1974439.0
46	2027762.5
47	2025415.5
48	1964423.0
49	1838256.0
50	1664859.0
51	1505181.0
52	1357439.0
53	1202418.0
54	1055455.5
55	910968.5
56	754645.5
57	603215.5
58	487676.5
59	404828.5
60	346761.5
61	303121.0
62	263899.5
63	225155.0
64	199133.5
65	176202.5
66	152890.0
67	130755.0
68	102264.5
69	77691.5
70	64111.5
71	53448.0
72	40421.0
73	28943.0
74	18284.0
75	10634.5
76	5652.0
77	2256.5
78	1100.5
79	655.5
80	450.0
81	342.5
82	288.5
83	232.0
84	189.5
85	153.5
86	143.5
87	138.5
88	107.5
89	91.5
90	86.5
91	74.5
92	64.5
93	65.0
94	65.5
95	57.0
96	51.5
97	64.0
98	87.5
99	138.0
100	690.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.007542972316542835
2	0.006234264991877225
3	0.0016928552458361132
4	1.3347512515246278E-4
5	0.014262305446169254
6	0.0013835836143852847
7	0.00491904668549686
8	0.009411624068677314
9	0.003161081622513204
10-11	0.006450755133892804
12-13	0.0044844386560370115
14-15	0.009182111963232226
16-17	9.603698029262564E-5
18-19	0.012553172746046254
20-21	0.006154505465871485
22-23	0.008430093575178106
24-25	0.01161396363369295
26-27	0.009538588212115022
28-29	0.006828392073348553
30-31	0.01054290714161587
32-33	0.005339005006098511
34-35	0.004879980795208334
36-37	0.008929811421785498
38-39	0.010355716417316686
40-41	0.008535897028042864
42-43	0.012211346206021656
44-45	0.007801783839704318
46-47	0.004219116151160775
48-49	0.009982962714147003
50-51	0.010098532639583891
52-53	0.007202773521946924
54-55	0.01036222739903144
56-57	0.012276456023169199
58-59	0.0057687297992722935
60-61	0.00252788865075335
62-63	0.01263944325376675
64-65	0.005151814281799325
66-67	0.006063351721864925
68-69	0.015138032486803704
70-71	0.006177293901873125
72-73	0.005799656962417376
74-75	0.013240081316952831
76-77	0.006494704260467396
78-79	0.012450624784038876
80-81	1.1394218000819993E-5
82-83	0.00707092614222315
84-85	0.016479294720043086
86-87	0.002137229747868093
88-89	0.009869020534138802
90-91	0.0035859231794009207
92-93	0.011527693125972455
94-95	0.010331300235886357
96-97	0.004129590152582903
98-99	0.009076308510367469
100-101	0.010139226275301104
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3.0717334E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.0865987094842
#Duplication Level	Percentage of deduplicated	Percentage of total
1	63.505785848715504	21.011904521068907
2	14.712589764220816	9.735791070120777
3	6.759357998299735	6.709324968704572
4	3.7993183368230583	5.028260847201978
5	2.435360162922289	4.028889221183692
6	1.6358454555763826	3.247473728363348
7	1.1601998668280091	2.6870947191574746
8	0.8923801801135987	2.3620659932572696
9	0.6978229689237104	2.0779729688735706
>10	3.972310113031468	24.018543968368913
>50	0.2754035517248454	6.2407323124878875
>100	0.140700363534765	8.547964909808314
>500	0.009061912800771376	2.071913911079899
>1k	0.0037814281293427334	2.05330379877267
>5k	7.179231099482529E-5	0.14123232007868122
>10k+	1.0256044427832183E-5	0.037530741472133936
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.9532945144262844E-5	0.0	1.3021963429508563E-5	6.5109817147542815E-6	3.2554908573771408E-6
2	2.6043926859017126E-5	1.6277454286885704E-5	1.3021963429508563E-5	6.5109817147542815E-6	3.2554908573771408E-6
3	3.906589028852569E-5	1.9532945144262844E-5	1.3021963429508563E-5	6.5109817147542815E-6	3.2554908573771408E-6
4	9.766472572131423E-5	1.9532945144262844E-5	3.255490857377141E-5	6.5109817147542815E-6	3.2554908573771408E-6
5	1.1719767086557708E-4	1.9532945144262844E-5	3.255490857377141E-5	6.5109817147542815E-6	3.2554908573771408E-6
6	1.595190520114799E-4	2.2788436001639987E-5	3.906589028852569E-5	6.5109817147542815E-6	6.5109817147542815E-6
7	1.6928552458361133E-4	2.2788436001639987E-5	5.208785371803425E-5	9.766472572131422E-6	6.5109817147542815E-6
8	1.920739605852513E-4	2.2788436001639987E-5	6.510981714754282E-5	9.766472572131422E-6	6.5109817147542815E-6
9	2.1160690572951415E-4	2.929941771639427E-5	9.115374400655995E-5	9.766472572131422E-6	9.766472572131422E-6
10-11	2.7671672287705697E-4	2.929941771639427E-5	1.2208090715164278E-4	9.766472572131422E-6	9.766472572131422E-6
12-13	3.125271223082055E-4	3.418265400245998E-5	1.4324159772459422E-4	9.766472572131422E-6	9.766472572131422E-6
14-15	3.450820308819769E-4	3.906589028852569E-5	1.6765777915492275E-4	1.6277454286885704E-5	9.766472572131422E-6
16-17	3.8414792117050266E-4	3.906589028852569E-5	2.4090632344590842E-4	2.2788436001639987E-5	1.3021963429508563E-5
18-19	4.606519563188654E-4	4.883236286065711E-5	3.2880457659509126E-4	2.929941771639427E-5	1.3021963429508563E-5
20-21	5.339005006098511E-4	4.883236286065711E-5	3.922866483139455E-4	3.255490857377141E-5	1.3021963429508563E-5
22-23	6.641201349049366E-4	5.534334457541139E-5	4.801849014631283E-4	3.418265400245998E-5	1.3021963429508563E-5
24-25	8.968877312074023E-4	7.324854429098567E-5	5.029733374647682E-4	3.581039943114855E-5	1.3021963429508563E-5
26-27	0.0012061593626582308	8.464276229180567E-5	5.143675554655882E-4	3.7438144859837114E-5	1.3021963429508563E-5
28-29	0.0015984460109721762	9.603698029262565E-5	5.599444274688682E-4	3.906589028852569E-5	1.6277454286885704E-5
30-31	0.002130718766153339	1.1719767086557706E-4	5.680831546123111E-4	4.2321381145902834E-5	1.6277454286885704E-5
32-33	0.002781816937628767	1.2370865258033134E-4	5.859883543278854E-4	4.557687200327997E-5	1.9532945144262844E-5
34-35	0.003509419144252558	1.2370865258033134E-4	6.917918071926423E-4	4.883236286065711E-5	2.4416181430328556E-5
36-37	0.004416073348032091	1.3021963429508563E-4	7.552738789114966E-4	4.883236286065711E-5	3.0927163145082835E-5
38-39	0.005568517111543599	1.3673061600983993E-4	7.796900603418253E-4	5.208785371803425E-5	3.255490857377141E-5
40-41	0.007157196649943644	1.3998610686721705E-4	8.008507509147766E-4	5.534334457541139E-5	3.255490857377141E-5
42-43	0.009009570947791238	1.4812483401065992E-4	8.56194095490188E-4	5.534334457541139E-5	3.581039943114855E-5
44-45	0.011114245787085558	1.595190520114799E-4	8.838657677778937E-4	5.534334457541139E-5	3.581039943114855E-5
46-47	0.013463082440683167	1.6277454286885704E-4	9.522310757828137E-4	5.534334457541139E-5	3.581039943114855E-5
48-49	0.016249782614598	1.660300337262342E-4	9.831582389278966E-4	5.534334457541139E-5	3.581039943114855E-5
50-51	0.019757574013421868	1.7254101544098845E-4	0.0010010634386434709	5.534334457541139E-5	3.581039943114855E-5
52-53	0.024155742161738387	1.7742425172705416E-4	0.0010238518746451108	5.859883543278854E-5	3.581039943114855E-5
54-55	0.029265235062391808	1.8067974258443133E-4	0.0010596622740762594	5.859883543278854E-5	4.720461743196854E-5
56-57	0.035206505877105096	1.9044621515656274E-4	0.0011003559097934735	5.859883543278854E-5	4.883236286065711E-5
58-59	0.04192095577044544	1.9532945144262845E-4	0.0011182611095090478	5.859883543278854E-5	5.208785371803425E-5
60-61	0.05036407130905306	1.9695719687131704E-4	0.0011736044540844592	5.859883543278854E-5	5.534334457541139E-5
62-63	0.061305776080697635	2.0184043315738274E-4	0.0012549917255188878	6.0226580861477104E-5	5.534334457541139E-5
64-65	0.07438471060021029	2.0509592401475986E-4	0.001341262233239382	6.185432629016567E-5	5.859883543278854E-5
66-67	0.09006966555105336	2.1160690572951415E-4	0.001364050669241022	6.185432629016567E-5	5.859883543278854E-5
68-69	0.10904917724956209	2.1323465115820274E-4	0.0013949778323861049	6.185432629016567E-5	5.859883543278854E-5
70-71	0.13226408255351849	2.2137337830164557E-4	0.0014031165595295477	6.185432629016567E-5	6.510981714754282E-5
72-73	0.16108494311387833	2.2137337830164557E-4	0.0014421824498180734	6.348207171885424E-5	7.813178057705137E-5
74-75	0.19699789050703426	2.2300112373033415E-4	0.0014454379406754504	6.510981714754282E-5	7.813178057705137E-5
76-77	0.2407679650844699	2.31139850873777E-4	0.0014568321586762706	6.510981714754282E-5	8.138727143442852E-5
78-79	0.29333112046768123	2.4090632344590842E-4	0.001466598631248402	6.510981714754282E-5	8.464276229180567E-5
80-81	0.3571843181442764	2.474173051606627E-4	0.001481248340106599	6.510981714754282E-5	8.464276229180567E-5
82-83	0.43522331723189256	2.474173051606627E-4	0.001495898048964796	6.510981714754282E-5	8.78982531491828E-5
84-85	0.5321799736917273	2.571837777327941E-4	0.0015121755032516818	6.510981714754282E-5	8.78982531491828E-5
86-87	0.6539288207759175	2.6043926859017127E-4	0.001526825212109879	6.510981714754282E-5	8.78982531491828E-5
88-89	0.799981534855857	2.6857799573361415E-4	0.0015382194301106992	6.510981714754282E-5	9.115374400655995E-5
90	0.9254579189717441	2.702057411623027E-4	0.0015463581572541418	6.836530800491996E-5	9.440923486393708E-5
>>END_MODULE
