##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	SRR19654111_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	27254776
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.665208769281392	32.0	32.0	32.0	32.0	32.0
2	31.701282079882073	32.0	32.0	32.0	32.0	32.0
3	35.82526845937021	37.0	37.0	37.0	32.0	37.0
4	36.389841435497395	37.0	37.0	37.0	37.0	37.0
5	36.56475041291846	37.0	37.0	37.0	37.0	37.0
6	40.187000326107984	41.0	41.0	41.0	37.0	41.0
7	40.22130550623494	41.0	41.0	41.0	37.0	41.0
8	40.25658930383431	41.0	41.0	41.0	41.0	41.0
9	40.30146085221907	41.0	41.0	41.0	41.0	41.0
10-11	40.331847287976245	41.0	41.0	41.0	41.0	41.0
12-13	40.31142826123392	41.0	41.0	41.0	41.0	41.0
14-15	40.306510756133164	41.0	41.0	41.0	41.0	41.0
16-17	40.30386501800639	41.0	41.0	41.0	41.0	41.0
18-19	40.30316365469304	41.0	41.0	41.0	41.0	41.0
20-21	40.27325601575298	41.0	41.0	41.0	41.0	41.0
22-23	40.279459754136305	41.0	41.0	41.0	41.0	41.0
24-25	40.26677401421314	41.0	41.0	41.0	41.0	41.0
26-27	40.188328625412296	41.0	41.0	41.0	41.0	41.0
28-29	40.197801185377564	41.0	41.0	41.0	41.0	41.0
30-31	40.1099173407259	41.0	41.0	41.0	39.0	41.0
32-33	40.13547686100961	41.0	41.0	41.0	41.0	41.0
34-35	40.15080320234516	41.0	41.0	41.0	41.0	41.0
36-37	40.13972292415832	41.0	41.0	41.0	41.0	41.0
38-39	40.067167585600416	41.0	41.0	41.0	37.0	41.0
40-41	40.085313707953425	41.0	41.0	41.0	37.0	41.0
42-43	40.074082043455434	41.0	41.0	41.0	37.0	41.0
44-45	40.07001897575677	41.0	41.0	41.0	37.0	41.0
46-47	40.05996928758468	41.0	41.0	41.0	37.0	41.0
48-49	39.96856162017255	41.0	41.0	41.0	37.0	41.0
50-51	39.93097367595316	41.0	41.0	41.0	37.0	41.0
52-53	39.97288400022073	41.0	41.0	41.0	37.0	41.0
54-55	39.94191216247751	41.0	41.0	41.0	37.0	41.0
56-57	39.93336065209268	41.0	41.0	41.0	37.0	41.0
58-59	39.93152053423591	41.0	41.0	41.0	37.0	41.0
60-61	39.91593205535793	41.0	41.0	41.0	37.0	41.0
62-63	39.870638746031155	41.0	41.0	41.0	37.0	41.0
64-65	39.81815566930361	41.0	41.0	41.0	37.0	41.0
66-67	39.79484456228883	41.0	41.0	41.0	37.0	41.0
68-69	39.77312941408874	41.0	41.0	41.0	37.0	41.0
70-71	39.750894503774305	41.0	41.0	41.0	37.0	41.0
72-73	39.726667740729184	41.0	41.0	41.0	37.0	41.0
74-75	39.70346303708385	41.0	41.0	41.0	37.0	41.0
76-77	39.16957809522999	41.0	39.0	41.0	34.5	41.0
78-79	39.4980311524116	41.0	41.0	41.0	37.0	41.0
80-81	39.681328677953545	41.0	41.0	41.0	37.0	41.0
82-83	39.75319496296723	41.0	41.0	41.0	37.0	41.0
84-85	39.791726980254765	41.0	41.0	41.0	37.0	41.0
86-87	39.800096595914056	41.0	41.0	41.0	37.0	41.0
88-89	39.68324085657501	41.0	41.0	41.0	37.0	41.0
90-91	39.70541027378101	41.0	41.0	41.0	37.0	41.0
92-93	39.6587935817194	41.0	41.0	41.0	37.0	41.0
94-95	39.65366923213752	41.0	41.0	41.0	37.0	41.0
96-97	39.61907461650024	41.0	41.0	41.0	37.0	41.0
98-99	39.57751122225331	41.0	41.0	41.0	37.0	41.0
100-101	39.06184009730992	41.0	39.0	41.0	34.5	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	1.0
16	6.0
17	16.0
18	41.0
19	146.0
20	416.0
21	1169.0
22	3003.0
23	6458.0
24	12263.0
25	21506.0
26	36060.0
27	55786.0
28	80987.0
29	113165.0
30	147138.0
31	186828.0
32	233805.0
33	282166.0
34	337874.0
35	412894.0
36	521827.0
37	707016.0
38	1101238.0
39	2606612.0
40	2.0386354E7
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.96428033452419	10.708766312353449	9.035712139316622	44.29124121380574
2	22.559635052586746	13.1886315998341	34.604324027465864	29.647409320113287
3	19.96207268710627	16.956011178228835	23.85317576577894	39.22874036888596
4	22.81317960565884	25.134776378275863	22.6455392625498	29.406504753515495
5	23.73517214010491	30.151111863843603	24.579857123023135	21.533858873028343
6	20.198742414907393	32.83183835376229	24.943228298775967	22.026190932554353
7	16.145900446952858	24.049102439880627	40.58099028221696	19.224006830949556
8	19.35285030410817	24.3691087389601	30.37616966655679	25.901871290374938
9	18.803368627942493	22.921024924218784	33.361598715762696	24.914007732076023
10-11	21.856367788351864	30.705056496544447	23.978117260547073	23.460458454556612
12-13	21.914817791934887	24.393735615365173	27.26104591723667	26.430400675463268
14-15	20.893044580516822	26.00992574659208	27.726736774501465	25.370292898389625
16-17	21.615846856662433	26.38563011507732	26.941928985486	25.056594042774243
18-19	21.44444353327122	26.777573448710612	26.69804453725447	25.079938480763698
20-21	21.652010642098105	26.590653322558953	26.813979318707297	24.943356716635645
22-23	21.633439585047405	26.850638948564466	26.748181676488553	24.767739789899576
24-25	21.584326354627905	26.551793578545563	26.552824591696318	25.31105547513021
26-27	21.386473695472677	26.72681477917852	26.739313506007168	25.147398019341637
28-29	21.51112991316545	26.882307109910013	26.66326969967204	24.943293277252497
30-31	21.606962983776537	26.617551590863137	26.624411060672166	25.15107436468816
32-33	21.654830331388524	26.535894112650197	26.7243858470897	25.08488970887158
34-35	21.735696892170388	26.703233591059416	26.526803962725655	25.034265554044545
36-37	21.60517664867251	26.5514124937222	26.47424436729915	25.36916649030614
38-39	21.720162659402266	26.652579195335846	26.616147851156107	25.011110294105777
40-41	21.860535562647808	26.86386598811159	26.496486340595865	24.77911210864474
42-43	21.845275851836025	26.464792812826637	26.514787720141232	25.175143615196106
44-45	21.803971164540116	26.488440044416432	26.660930913539705	25.046657877503748
46-47	21.781987203430813	26.612202238335552	26.545957354064587	25.05985320416905
48-49	21.707345897834568	26.464273637765356	26.470525752990966	25.35785471140911
50-51	21.775166832673936	26.487832932666088	26.586129245488472	25.150870989171498
52-53	22.105849885103147	26.598363615826702	26.34610770373298	24.94967879533717
54-55	21.8690863575617	26.528998073585342	26.382522828292554	25.21939274056041
56-57	21.81938258355158	26.405933548102077	26.714543990605033	25.06013987774131
58-59	21.935848862142233	26.449923135406888	26.604536571272092	25.009691431178787
60-61	21.902405472509674	26.305606502329397	26.53020261366948	25.26178541149145
62-63	21.917756222009693	26.294175631300597	26.6398790189498	25.14818912773991
64-65	22.02929147167742	26.53816844406939	26.48345226938843	24.949087814864754
66-67	21.9158050836427	26.36233354442361	26.490143483112156	25.231717888821535
68-69	21.9571428170449	26.342023882169684	26.681268315544067	25.019564985241345
70-71	22.16163844091466	26.61757430247228	26.437466721145658	24.783320535467407
72-73	22.11761527594283	26.399677619805058	26.32464122985271	25.15806587439941
74-75	22.040149953901658	26.372377817377767	26.559009327392747	25.028462901327824
76-77	22.017032505803115	26.541641228238234	26.459021945835175	24.98230432012348
78-79	21.99923642739233	26.343201762935088	26.43348318902865	25.22407862064393
80-81	22.0882357506021	26.40402017631916	26.52983872982811	24.977905343250633
82-83	22.206729973666725	26.61437803459173	26.292715438813936	24.88617655292761
84-85	22.26169441645801	26.45135010222706	26.215728957091343	25.071226524223583
86-87	22.24127268139877	26.369288954076037	26.459569393619287	24.929868970905908
88-89	22.4255466271306	26.456368601231578	26.384296829297	24.733787942340822
90-91	22.32341746536333	26.31771809643382	26.299564961356204	25.05929947684664
92-93	22.28500945789532	26.360358103354613	26.256101841319435	25.098530597430628
94-95	22.483516283531372	26.531777402977003	26.10610888895216	24.878597424539464
96-97	22.473496189934266	26.54706496914745	25.897636018078117	25.081802822840167
98-99	22.49931469979668	26.59934170820143	26.155988892688057	24.745354699313825
100-101	22.600152721856894	26.735897590939654	25.938589625539393	24.725360061664052
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	76.0
1	54.0
2	34.0
3	41.5
4	46.0
5	53.0
6	58.0
7	66.5
8	78.0
9	94.5
10	125.5
11	155.5
12	181.5
13	193.5
14	227.5
15	283.5
16	341.0
17	438.5
18	542.5
19	696.5
20	978.0
21	1371.0
22	1914.5
23	2865.5
24	4604.0
25	6828.5
26	9714.0
27	14269.0
28	20885.0
29	30142.5
30	43221.5
31	59850.5
32	83016.5
33	113897.5
34	152622.5
35	207007.0
36	280954.5
37	377459.5
38	504672.0
39	668120.5
40	854523.0
41	1050135.0
42	1251014.5
43	1438858.5
44	1590354.0
45	1686921.5
46	1741259.0
47	1749011.0
48	1687470.0
49	1579965.0
50	1447209.5
51	1294573.5
52	1161866.0
53	1043211.5
54	903759.5
55	761639.5
56	638119.0
57	525887.0
58	420628.0
59	335054.0
60	272636.5
61	232379.0
62	199032.5
63	164386.0
64	139220.0
65	121447.5
66	103822.5
67	84680.0
68	59669.0
69	39211.5
70	28521.0
71	20582.5
72	14843.0
73	11290.5
74	7298.5
75	3572.5
76	1775.5
77	645.0
78	84.5
79	30.0
80	18.0
81	9.0
82	2.0
83	3.0
84	1.5
85	0.5
86	1.5
87	2.5
88	1.5
89	0.0
90	0.0
91	0.0
92	0.0
93	0.5
94	0.5
95	1.5
96	1.5
97	0.0
98	0.5
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.060345386804866785
2	0.0
3	4.035989875682706E-5
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	5.30182306396501E-4
12-13	0.0
14-15	0.0
16-17	0.0013263730364175437
18-19	0.001968462334821611
20-21	0.0
22-23	0.0
24-25	1.1557607371273202E-4
26-27	0.0
28-29	2.6050480106679287E-4
30-31	0.0017721664635952246
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.002832531076388226
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0021867726962789937
48-49	0.0
50-51	0.0016419140630618283
52-53	4.127716918311858E-4
54-55	0.0
56-57	6.952909831289752E-4
58-59	1.0823791030239985E-4
60-61	0.0010897172664343308
62-63	0.003379224250457975
64-65	0.0018161954440572177
66-67	8.970904769131105E-4
68-69	3.3572097602269785E-4
70-71	8.512269555985344E-4
72-73	0.0
74-75	0.0
76-77	8.897523135027784E-4
78-79	1.834540852583048E-6
80-81	0.0016474176856195774
82-83	0.0010493573676775036
84-85	2.3849031083579627E-5
86-87	9.411194573751037E-4
88-89	0.0
90-91	0.0019464478445906143
92-93	0.0010933863481394968
94-95	0.0
96-97	2.2931760657288102E-4
98-99	0.0010897172664343308
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2.7254776E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.839699624092816
#Duplication Level	Percentage of deduplicated	Percentage of total
1	63.40395268280912	21.455707137664547
2	15.435990434032831	10.446985593760823
3	6.7682651850122	6.871081825110535
4	3.6518139646244516	4.9430515058383575
5	2.3104328595751027	3.909217698482765
6	1.5723902245144028	3.192552773365635
7	1.14536973073811	2.7131277352693997
8	0.838138881841744	2.2689894403838107
9	0.6667383511023623	2.030600297625019
>10	3.7899914708124065	23.434512575222552
>50	0.2650412435585565	6.1598989559721815
>100	0.1399547166214006	8.718129936822965
>500	0.008503769855186721	1.9836672066391523
>1k	0.0033934396399349304	1.829351903554314
>5k	2.304526197532326E-5	0.043125414288091725
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.5036225577491446E-5	3.6690817051660966E-6	0.0	0.0	0.0
2	7.338163410332193E-5	3.6690817051660966E-6	0.0	0.0	0.0
3	1.064033694498168E-4	3.6690817051660966E-6	0.0	0.0	0.0
4	1.5777051332214215E-4	3.6690817051660966E-6	7.338163410332193E-6	0.0	3.6690817051660966E-6
5	1.6510867673247433E-4	7.338163410332193E-6	1.1007245115498289E-5	0.0	3.6690817051660966E-6
6	1.981304120789692E-4	7.338163410332193E-6	2.5683571936162673E-5	0.0	3.6690817051660966E-6
7	2.2748306572029797E-4	7.338163410332193E-6	2.5683571936162673E-5	0.0	7.338163410332193E-6
8	2.935265364132877E-4	1.1007245115498289E-5	2.5683571936162673E-5	0.0	7.338163410332193E-6
9	3.155410266442843E-4	1.1007245115498289E-5	2.5683571936162673E-5	0.0	1.1007245115498289E-5
10-11	3.3388643517011477E-4	2.2014490230996578E-5	2.5683571936162673E-5	0.0	1.1007245115498289E-5
12-13	3.907572016001893E-4	4.035989875682706E-5	2.5683571936162673E-5	0.0	1.2841785968081337E-5
14-15	4.916569484922569E-4	4.7698062167159254E-5	2.5683571936162673E-5	0.0	1.4676326820664386E-5
16-17	5.72376746005911E-4	5.5036225577491446E-5	2.5683571936162673E-5	0.0	2.0179949378413528E-5
18-19	6.549310843721482E-4	5.8705307282657545E-5	2.5683571936162673E-5	0.0	2.2014490230996578E-5
20-21	7.374854227383853E-4	5.8705307282657545E-5	3.6690817051660964E-5	0.0	2.3849031083579627E-5
22-23	8.310470062201209E-4	6.053984813524059E-5	3.6690817051660964E-5	0.0	2.5683571936162673E-5
24-25	9.374503756699376E-4	6.420892984040669E-5	3.6690817051660964E-5	0.0	2.5683571936162673E-5
26-27	0.0010860481847291647	8.989250177656935E-5	4.035989875682706E-5	0.0	2.9352653641328773E-5
28-29	0.001377740180289869	9.723066518690156E-5	4.4028980461993155E-5	0.0	2.9352653641328773E-5
30-31	0.001790511872121055	9.90652060394846E-5	4.58635213145762E-5	0.0	3.3021735346494865E-5
32-33	0.0022656579529400644	1.027342877446507E-4	4.95326030197423E-5	0.0	3.3021735346494865E-5
34-35	0.0028655528117347215	1.1374153286014898E-4	5.136714387232535E-5	0.0	3.3021735346494865E-5
36-37	0.003694765277102259	1.1741061456531509E-4	5.136714387232535E-5	0.0	3.3021735346494865E-5
38-39	0.004923907648332901	1.1741061456531509E-4	5.136714387232535E-5	7.338163410332193E-6	3.3021735346494865E-5
40-41	0.006793304777115028	1.2658331882823033E-4	5.136714387232535E-5	1.1007245115498289E-5	3.3021735346494865E-5
42-43	0.009427705441424284	1.339214822385625E-4	5.136714387232535E-5	1.1007245115498289E-5	3.3021735346494865E-5
44-45	0.012496892287795724	1.485978090592269E-4	5.136714387232535E-5	1.1007245115498289E-5	3.6690817051660964E-5
46-47	0.015777051332214215	1.6143959502730825E-4	5.136714387232535E-5	1.1007245115498289E-5	3.6690817051660964E-5
48-49	0.020051531518732714	1.6143959502730825E-4	5.136714387232535E-5	1.1007245115498289E-5	3.852535790424402E-5
50-51	0.02589637867506231	1.6510867673247433E-4	5.136714387232535E-5	1.1007245115498289E-5	4.219443960941011E-5
52-53	0.033786738882021994	1.6877775843764044E-4	5.136714387232535E-5	1.4676326820664386E-5	4.4028980461993155E-5
54-55	0.04280534171332026	1.6877775843764044E-4	5.32016847249084E-5	1.4676326820664386E-5	4.4028980461993155E-5
56-57	0.05282010022757112	2.017994937841353E-4	5.5036225577491446E-5	1.4676326820664386E-5	5.5036225577491446E-5
58-59	0.0643813766805495	2.1097219804705055E-4	5.5036225577491446E-5	1.4676326820664386E-5	5.5036225577491446E-5
60-61	0.07932371192483842	2.3298668827804713E-4	5.687076643007449E-5	1.4676326820664386E-5	5.5036225577491446E-5
62-63	0.09847815296665803	2.3482122913063018E-4	6.053984813524059E-5	1.4676326820664386E-5	5.5036225577491446E-5
64-65	0.12269225767990169	2.4215939254096236E-4	6.237438898782364E-5	1.4676326820664386E-5	5.8705307282657545E-5
66-67	0.1510762737510666	2.4582847424612844E-4	6.237438898782364E-5	1.4676326820664386E-5	5.8705307282657545E-5
68-69	0.18489236528672992	2.476630150987115E-4	6.237438898782364E-5	1.4676326820664386E-5	5.8705307282657545E-5
70-71	0.2261273400302391	2.4949755595129455E-4	6.237438898782364E-5	1.4676326820664386E-5	5.8705307282657545E-5
72-73	0.2779476154931525	2.5316663765646066E-4	6.237438898782364E-5	1.4676326820664386E-5	5.8705307282657545E-5
74-75	0.3436131707705101	2.7701566874004026E-4	6.237438898782364E-5	1.4676326820664386E-5	5.8705307282657545E-5
76-77	0.42382480046799875	3.192101083494504E-4	6.237438898782364E-5	1.4676326820664386E-5	5.8705307282657545E-5
78-79	0.5221543556255974	3.210446492020334E-4	6.237438898782364E-5	1.4676326820664386E-5	5.8705307282657545E-5
80-81	0.639475077689136	3.2471373090719953E-4	6.604347069298973E-5	1.8345408525830482E-5	6.237438898782364E-5
82-83	0.7823858101053555	3.320518943175317E-4	6.971255239815582E-5	1.8345408525830482E-5	6.971255239815584E-5
84-85	0.9594978876362807	3.7057725222177577E-4	7.338163410332193E-5	1.8345408525830482E-5	6.971255239815584E-5
86-87	1.1814956762073554	3.7791541563210793E-4	7.338163410332193E-5	1.8345408525830482E-5	6.971255239815584E-5
88-89	1.447443559983762	3.889226607476062E-4	7.338163410332193E-5	1.8345408525830482E-5	7.338163410332193E-5
90	1.6760768828186297	3.962608241579384E-4	7.338163410332193E-5	1.8345408525830482E-5	7.338163410332193E-5
>>END_MODULE
