##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	SRR19654119_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	32083105
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.46622379598234	32.0	32.0	32.0	32.0	32.0
2	31.444420357692934	32.0	32.0	32.0	32.0	32.0
3	35.383139973515654	37.0	37.0	37.0	32.0	37.0
4	35.99543061683088	37.0	37.0	37.0	32.0	37.0
5	36.17222114256086	37.0	37.0	37.0	37.0	37.0
6	39.414064224768765	41.0	41.0	41.0	37.0	41.0
7	39.52931906060838	41.0	41.0	41.0	37.0	41.0
8	39.680126907916176	41.0	41.0	41.0	37.0	41.0
9	39.772975901179144	41.0	41.0	41.0	37.0	41.0
10-11	39.81589629495026	41.0	41.0	41.0	37.0	41.0
12-13	39.76124126701577	41.0	41.0	41.0	37.0	41.0
14-15	39.69631017633736	41.0	41.0	41.0	37.0	41.0
16-17	39.71197515016081	41.0	41.0	41.0	37.0	41.0
18-19	39.64678876935384	41.0	41.0	41.0	37.0	41.0
20-21	39.67358754709059	41.0	41.0	41.0	37.0	41.0
22-23	39.66476371286383	41.0	41.0	41.0	37.0	41.0
24-25	39.61831823945968	41.0	41.0	41.0	37.0	41.0
26-27	39.52717328949302	41.0	41.0	41.0	37.0	41.0
28-29	39.549842198876945	41.0	41.0	41.0	37.0	41.0
30-31	39.59449923877381	41.0	41.0	41.0	37.0	41.0
32-33	39.577192933788666	41.0	41.0	41.0	37.0	41.0
34-35	39.48133305675994	41.0	41.0	41.0	37.0	41.0
36-37	39.40025477583919	41.0	41.0	41.0	37.0	41.0
38-39	39.344587174464564	41.0	41.0	41.0	37.0	41.0
40-41	39.33976215830731	41.0	41.0	41.0	37.0	41.0
42-43	39.29771460711175	41.0	41.0	41.0	37.0	41.0
44-45	39.282715248415016	41.0	41.0	41.0	37.0	41.0
46-47	39.232870571598355	41.0	41.0	41.0	37.0	41.0
48-49	39.198918948150435	41.0	41.0	41.0	37.0	41.0
50-51	39.1964671748573	41.0	41.0	41.0	37.0	41.0
52-53	39.208124603276396	41.0	41.0	41.0	37.0	41.0
54-55	39.20104059441877	41.0	41.0	41.0	37.0	41.0
56-57	39.16192661215303	41.0	41.0	41.0	37.0	41.0
58-59	39.131488660464754	41.0	41.0	41.0	37.0	41.0
60-61	39.08264533622915	41.0	41.0	41.0	37.0	41.0
62-63	39.018013593135706	41.0	41.0	41.0	37.0	41.0
64-65	39.03546492772442	41.0	41.0	41.0	37.0	41.0
66-67	39.02206387131171	41.0	41.0	41.0	37.0	41.0
68-69	38.967817874859676	41.0	41.0	41.0	37.0	41.0
70-71	38.89300722296049	41.0	41.0	41.0	32.0	41.0
72-73	38.90402149355556	41.0	41.0	41.0	32.0	41.0
74-75	38.801050100979936	41.0	41.0	41.0	32.0	41.0
76-77	37.89781548575177	41.0	39.0	41.0	29.5	41.0
78-79	38.44284346854832	41.0	39.0	41.0	32.0	41.0
80-81	38.90537940451836	41.0	41.0	41.0	34.5	41.0
82-83	38.981500683926946	41.0	41.0	41.0	37.0	41.0
84-85	38.94844347203926	41.0	41.0	41.0	37.0	41.0
86-87	38.90310836497901	41.0	41.0	41.0	32.0	41.0
88-89	38.830045128113376	41.0	41.0	41.0	32.0	41.0
90-91	38.714714239784456	41.0	41.0	41.0	32.0	41.0
92-93	38.628763191717255	41.0	41.0	41.0	32.0	41.0
94-95	38.495744271011176	41.0	41.0	41.0	32.0	41.0
96-97	38.52544705383098	41.0	41.0	41.0	32.0	41.0
98-99	38.46789263383329	41.0	37.0	41.0	32.0	41.0
100-101	37.41857670883164	41.0	37.0	41.0	29.5	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	44.0
13	155.0
14	538.0
15	1714.0
16	6421.0
17	16736.0
18	28850.0
19	39146.0
20	48277.0
21	58672.0
22	70989.0
23	84191.0
24	99136.0
25	116029.0
26	133755.0
27	156167.0
28	181703.0
29	212777.0
30	248980.0
31	295404.0
32	348311.0
33	415257.0
34	509842.0
35	642203.0
36	844396.0
37	1200591.0
38	2003734.0
39	5131177.0
40	1.918791E7
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	33.762378694545156	18.68108524936518	13.147074394277809	34.40946166181186
2	29.791195696586026	21.975553839618456	30.661572095687443	17.571678368108074
3	22.197150348901086	25.39772386358766	28.509913777989038	23.89521200952221
4	25.19902389396364	30.766833590066632	22.416021404762336	21.61812111120739
5	27.383348248805532	32.46703177781042	21.8591107519318	18.290509221452247
6	21.802075165973225	36.1996382166296	21.94844672687662	20.049839890520555
7	21.6467732783345	19.621860165965856	37.17024271809103	21.561123837608612
8	23.082868190730686	23.959001938802576	26.257054144930947	26.701075725535787
9	23.602743562382756	23.02556750663628	28.762820805529888	24.608868125451075
10-11	25.624463405272024	29.289895725491654	22.43124535483707	22.654395514399244
12-13	26.200221414615406	23.72218341355356	25.56391077047729	24.513684401353746
14-15	24.71881851834478	26.48006637761526	26.10546111419079	22.695653989849173
16-17	25.740297134195977	25.94200816752877	25.37820253805028	22.939492160224972
18-19	25.367044430394124	26.655531002999865	25.37238992298283	22.605034643623178
20-21	25.54170787813701	26.400574011203286	25.277828232851373	22.779889877808333
22-23	25.417634946345437	26.305443398040396	25.464754765148495	22.812166890465676
24-25	25.142919106950913	26.22268707760175	25.759625477547033	22.874768337900303
26-27	25.332928591480275	26.453062112029315	25.461844596708733	22.752164699781684
28-29	25.46232827663266	26.297299638369832	25.368709510401793	22.871662574595707
30-31	25.09893389155142	26.208277021130165	25.736672709319315	22.95611637799909
32-33	25.241234702513644	26.477267517059754	25.523636888072105	22.7578608923545
34-35	25.36343037869932	26.427434314727332	25.35899190555278	22.850143401020567
36-37	25.110993776942724	26.31690885280586	25.77526240056877	22.796834969682642
38-39	25.45170547561983	26.35363666313108	25.51996629847849	22.6746915627706
40-41	25.583915584230393	26.150065587479766	25.282778895621234	22.98323993266861
42-43	25.096058426813038	26.279648106082586	25.81099138929251	22.813302077811862
44-45	25.19448436232955	26.449946525410965	25.77435917768076	22.581209934578723
46-47	25.59995985425974	26.08314407224612	25.517050173292144	22.79984590020199
48-49	25.208603391016425	26.123180215899854	25.8450626213818	22.823153771701914
50-51	25.435111984567783	26.233740618185543	25.541413669181967	22.78973372806471
52-53	25.598208240126148	26.165135379641296	25.462944470632703	22.773711909599857
54-55	25.04376558211349	26.173519947209194	25.93635124600336	22.84636322467396
56-57	25.169512958619904	26.341231253778304	25.710858116964353	22.77839767063744
58-59	25.524441434464023	26.059447688263553	25.58986102750824	22.826249849764185
60-61	25.36031347339979	26.187000915279242	25.645874986227174	22.8068106250938
62-63	25.305690698220772	26.237898449291492	25.742177063942307	22.714233788545428
64-65	25.494711710333394	26.198049194397633	25.569844713749568	22.737394381519408
66-67	25.214236876725515	26.320242248191438	25.89262956134057	22.57289131374248
68-69	25.399898426027324	26.50678605026671	25.597437485782983	22.495878037922985
70-71	25.716298746439207	26.277215780389213	25.303395835943093	22.703089637228487
72-73	25.21188922320885	26.346173950235013	25.809365372443843	22.632571454112295
74-75	25.210335011375445	26.51976983832101	25.645684960992725	22.624210189310823
76-77	25.763666585803524	26.29108120237959	25.32838016180089	22.616872050016003
78-79	25.255557646831033	26.480982911041227	25.594515704262367	22.668943737865373
80-81	25.46327424027812	26.429027818008183	25.61311108467238	22.494586857041316
82-83	25.686940196696757	26.220789804040272	25.50164599200094	22.590624007262026
84-85	25.461512546705755	26.401298124404484	25.69847987729664	22.43870945159312
86-87	25.419173113076184	26.74883088778346	25.3849557266979	22.447040272442457
88-89	25.759328780677553	26.344566711981276	25.466380202290274	22.4297243050509
90-91	25.397689843299144	26.60709117773981	25.658492218879687	22.33672676008136
92-93	25.618131412156025	26.705907984903583	25.430919170697475	22.24504143224292
94-95	26.035608730543913	26.524604692809017	25.183298290466876	22.256488286180193
96-97	25.827933193053433	26.539740537668134	25.55648765861675	22.075838610661684
98-99	25.98697040077636	26.815007462650513	25.15823671056776	22.03978542600537
100-101	26.226206331292968	26.67125103212899	25.1137543213364	21.988788315241642
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	39.0
1	28.0
2	19.5
3	19.0
4	21.5
5	28.5
6	37.0
7	43.0
8	52.0
9	66.5
10	94.5
11	133.0
12	142.5
13	163.0
14	209.0
15	280.5
16	368.5
17	447.0
18	556.5
19	695.5
20	863.5
21	1107.0
22	1415.5
23	1933.5
24	2627.5
25	3516.5
26	4892.0
27	6768.5
28	9265.5
29	13165.5
30	18724.5
31	26437.5
32	38208.0
33	55569.0
34	83166.5
35	127889.5
36	193012.5
37	286448.0
38	424899.0
39	622832.5
40	855789.5
41	1105196.5
42	1375953.0
43	1613159.0
44	1818175.0
45	2010860.0
46	2095208.0
47	2108573.0
48	2085355.5
49	1970636.5
50	1816157.5
51	1680035.0
52	1514355.5
53	1309810.5
54	1139350.5
55	987327.0
56	834930.0
57	695201.5
58	565889.5
59	439955.5
60	362853.5
61	333578.0
62	304248.5
63	254576.5
64	195125.0
65	157833.5
66	137085.0
67	114808.0
68	83836.0
69	58359.5
70	44113.5
71	32711.0
72	22383.5
73	14428.5
74	7544.0
75	3436.0
76	1722.0
77	1010.5
78	692.0
79	540.5
80	438.0
81	339.5
82	267.5
83	233.0
84	203.0
85	165.0
86	144.0
87	128.5
88	111.5
89	99.5
90	83.0
91	73.5
92	74.5
93	66.5
94	53.0
95	51.5
96	60.5
97	73.0
98	88.0
99	137.5
100	667.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.017488955635684263
2	0.006723164731094451
3	7.168882188927786E-4
4	0.0016363752822552557
5	2.7740457165850995E-4
6	0.0011532549608275135
7	0.0
8	0.023803805772539783
9	0.0
10-11	0.0
12-13	0.0037776892230349897
14-15	0.0
16-17	0.0010239033908968599
18-19	0.0
20-21	3.3506731969988563E-4
22-23	6.23381059906764E-6
24-25	2.6493695046037474E-5
26-27	0.005457701179483719
28-29	0.001022344938247093
30-31	2.0259884446969828E-4
32-33	4.301329313356672E-4
34-35	0.0
36-37	0.0
38-39	8.742919365192366E-4
40-41	0.0
42-43	8.415644308741314E-5
44-45	0.001798454357831014
46-47	0.0
48-49	0.0014119581006888204
50-51	0.0011174105498828746
52-53	7.309142927406808E-4
54-55	7.667587036853197E-4
56-57	0.008541878973372434
58-59	6.514332076025684E-4
60-61	0.0
62-63	0.0049122427520653005
64-65	1.55845264976691E-6
66-67	0.0024981995975763564
68-69	2.587031398613071E-4
70-71	5.610429539160876E-5
72-73	2.0883265506876594E-4
74-75	5.594845012663207E-4
76-77	2.8675528755711145E-4
78-79	2.4779397131293867E-4
80-81	0.0016893626723473304
82-83	0.0052722453141614565
84-85	6.280564178560647E-4
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	5.610429539160876E-5
96-97	0.004771982013586278
98-99	0.0
100-101	4.613019843310054E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3.2083105E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.08041439506192
#Duplication Level	Percentage of deduplicated	Percentage of total
1	62.134914119117	16.205043086281552
2	14.477108971300346	7.551380024279631
3	6.80114390229867	5.321299539971935
4	3.894465389758938	4.062770848485577
5	2.557394710532646	3.3348956911215413
6	1.7622508331853832	2.7576139198510754
7	1.269267772817119	2.3172120645357444
8	0.9494357185309819	1.98093415846091
9	0.7812959126338311	1.8338869049992563
>10	4.6732813997245755	22.937580882425664
>50	0.3926297742910356	7.108185799791531
>100	0.26812744645509157	13.655643480049312
>500	0.02570224202335582	4.571775048700726
>1k	0.01269104413285217	5.804266464959777
>5k	2.654794419577828E-4	0.4347759457340599
>10k+	2.5283756376931698E-5	0.12273614035168588
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.55845264976691E-5	3.11690529953382E-6	6.23381059906764E-6	3.11690529953382E-6	0.0
2	2.181833709673674E-5	1.55845264976691E-5	9.35071589860146E-6	9.35071589860146E-6	0.0
3	4.987048479254112E-5	1.55845264976691E-5	1.55845264976691E-5	9.35071589860146E-6	1.246762119813528E-5
4	1.3714383317948807E-4	1.55845264976691E-5	2.181833709673674E-5	9.35071589860146E-6	1.246762119813528E-5
5	1.5896217027622483E-4	1.55845264976691E-5	2.805214769580438E-5	9.35071589860146E-6	1.246762119813528E-5
6	1.714297914743601E-4	1.55845264976691E-5	3.740286359440584E-5	9.35071589860146E-6	1.246762119813528E-5
7	1.870143179720292E-4	1.55845264976691E-5	5.610429539160876E-5	1.246762119813528E-5	1.55845264976691E-5
8	2.3376789746503652E-4	1.55845264976691E-5	6.23381059906764E-5	1.246762119813528E-5	1.55845264976691E-5
9	2.5558623456177325E-4	1.870143179720292E-5	7.480572718881168E-5	1.246762119813528E-5	1.55845264976691E-5
10-11	2.7740457165851E-4	1.870143179720292E-5	7.79226324883455E-5	1.246762119813528E-5	3.428595829487202E-5
12-13	3.1792434055244966E-4	2.3376789746503648E-5	8.883180103671387E-5	1.246762119813528E-5	3.7402863594405844E-5
14-15	4.0987304688869735E-4	3.7402863594405844E-5	1.2779311728088662E-4	1.246762119813528E-5	4.0519768893939664E-5
16-17	4.862372267272759E-4	4.363667419347348E-5	2.2909253951573577E-4	1.246762119813528E-5	4.363667419347348E-5
18-19	5.672767645151552E-4	4.987048479254112E-5	2.4779397131293867E-4	1.55845264976691E-5	4.363667419347348E-5
20-21	6.670177341002375E-4	5.610429539160876E-5	2.6182004516084084E-4	1.870143179720292E-5	4.363667419347348E-5
22-23	8.384475255745975E-4	6.0779653340909485E-5	3.0545671935431435E-4	2.025988444696983E-5	4.363667419347348E-5
24-25	0.0010722154230396342	6.701346393997714E-5	3.225996985017504E-4	2.181833709673674E-5	4.363667419347348E-5
26-27	0.0013979320268409183	7.324727453904476E-5	3.45976488248254E-4	2.181833709673674E-5	4.363667419347348E-5
28-29	0.0017766360207342774	7.79226324883455E-5	3.662363726952239E-4	2.181833709673674E-5	4.67535794930073E-5
30-31	0.002326769806101997	8.103953778787933E-5	3.740286359440584E-4	2.181833709673674E-5	5.922120069114258E-5
32-33	0.003020281235248272	8.259799043764624E-5	3.771455412435922E-4	2.181833709673674E-5	6.23381059906764E-5
34-35	0.0039366513933112145	8.415644308741314E-5	4.535097210821708E-4	2.181833709673674E-5	6.701346393997714E-5
36-37	0.005016659079599683	8.571489573718005E-5	4.6441888963053924E-4	2.181833709673674E-5	7.79226324883455E-5
38-39	0.006589137803214495	9.039025368648078E-5	4.7532805817890756E-4	2.181833709673674E-5	8.727334838694697E-5
40-41	0.008819283545030945	9.506561163578152E-5	5.174062797226142E-4	2.181833709673674E-5	8.883180103671387E-5
42-43	0.011808395727283878	9.662406428554842E-5	5.563675959667869E-4	2.181833709673674E-5	1.0597478018414988E-4
44-45	0.015246342272669682	1.075332328339168E-4	5.657183118653883E-4	2.181833709673674E-5	1.0597478018414988E-4
46-47	0.018991303990059563	1.0909168548368371E-4	6.187057019574633E-4	2.181833709673674E-5	1.1376704343298443E-4
48-49	0.0236947140870561	1.1376704343298443E-4	6.389655864044331E-4	2.181833709673674E-5	1.1844240138228516E-4
50-51	0.030427229534049152	1.2000085403205207E-4	6.623423761509368E-4	2.805214769580438E-5	1.1844240138228516E-4
52-53	0.03923092855258242	1.231177593315859E-4	6.935114291462749E-4	2.805214769580438E-5	1.1844240138228516E-4
54-55	0.04923463611143622	1.246762119813528E-4	7.27797387441147E-4	2.961060034557129E-5	1.1844240138228516E-4
56-57	0.060207701218445035	1.246762119813528E-4	7.776678722336882E-4	3.11690529953382E-5	1.246762119813528E-4
58-59	0.0730290911680774	1.2935156993065355E-4	8.103953778787932E-4	3.11690529953382E-5	1.2779311728088662E-4
60-61	0.08956583223475409	1.3091002258042045E-4	8.384475255745976E-4	3.11690529953382E-5	1.2779311728088662E-4
62-63	0.11122832406651413	1.3091002258042045E-4	9.02344084215041E-4	3.11690529953382E-5	1.2935156993065355E-4
64-65	0.13758955063732142	1.3246847523018735E-4	9.428638531089805E-4	3.11690529953382E-5	1.3246847523018735E-4
66-67	0.16864327813657687	1.4337764377855573E-4	9.506561163578151E-4	3.11690529953382E-5	1.3402692787995427E-4
68-69	0.20459833921934922	1.6207907557575865E-4	9.740329061043189E-4	3.11690529953382E-5	1.3558538052972117E-4
70-71	0.24816332459093343	1.6519598087529245E-4	9.771498114038526E-4	3.11690529953382E-5	1.4649454907808955E-4
72-73	0.3029102077245952	1.8389741267249538E-4	9.849420746526872E-4	3.11690529953382E-5	1.5116990702739025E-4
74-75	0.3727055096444063	1.8389741267249538E-4	9.86500527302454E-4	3.2727505645105105E-5	1.574037176264579E-4
76-77	0.4600147024422979	1.854558653222623E-4	9.927343379015218E-4	3.428595829487202E-5	1.6207907557575863E-4
78-79	0.5658648064144665	1.9792348652039756E-4	9.989681485005892E-4	3.428595829487202E-5	1.6519598087529245E-4
80-81	0.6934225973452383	2.010403918199314E-4	0.0010161111276480253	3.428595829487202E-5	1.6519598087529245E-4
82-83	0.84578627910235	2.057157497692321E-4	0.001036371012094995	3.428595829487202E-5	1.6831288617482628E-4
84-85	1.0351569774808267	2.07274202418999E-4	0.0010488386332931305	3.428595829487202E-5	1.6831288617482628E-4
86-87	1.2733384128500032	2.1194956036829976E-4	0.0010706569703898671	3.428595829487202E-5	1.714297914743601E-4
88-89	1.5594142150518162	2.2130027626690121E-4	0.0010877999495373031	3.428595829487202E-5	1.714297914743601E-4
90	1.805021677297132	2.3688480276457032E-4	0.0010940337601363708	3.428595829487202E-5	1.714297914743601E-4
>>END_MODULE
