##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	SRR19654138_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	38008707
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.49280376730521	32.0	32.0	32.0	32.0	32.0
2	31.41021947944717	32.0	32.0	32.0	32.0	32.0
3	35.127094799620515	37.0	32.0	37.0	32.0	37.0
4	35.98234712377877	37.0	37.0	37.0	32.0	37.0
5	36.194979060982	37.0	37.0	37.0	37.0	37.0
6	39.27518602513892	41.0	41.0	41.0	37.0	41.0
7	39.50324868983309	41.0	41.0	41.0	37.0	41.0
8	39.64202670719633	41.0	41.0	41.0	37.0	41.0
9	39.75528914993083	41.0	41.0	41.0	37.0	41.0
10-11	39.80525135990551	41.0	41.0	41.0	37.0	41.0
12-13	39.74976319241799	41.0	41.0	41.0	37.0	41.0
14-15	39.72534331409906	41.0	41.0	41.0	37.0	41.0
16-17	39.67706320554393	41.0	41.0	41.0	37.0	41.0
18-19	39.70481332080041	41.0	41.0	41.0	37.0	41.0
20-21	39.62542798417215	41.0	41.0	41.0	37.0	41.0
22-23	39.55148857076354	41.0	41.0	41.0	37.0	41.0
24-25	39.574209167388936	41.0	41.0	41.0	37.0	41.0
26-27	39.379377270055514	41.0	41.0	41.0	37.0	41.0
28-29	39.473625030180585	41.0	41.0	41.0	37.0	41.0
30-31	39.551290326713826	41.0	41.0	41.0	37.0	41.0
32-33	39.541434282413235	41.0	41.0	41.0	37.0	41.0
34-35	39.3379170856825	41.0	41.0	41.0	37.0	41.0
36-37	39.21256016417502	41.0	41.0	41.0	37.0	41.0
38-39	39.2633758101795	41.0	41.0	41.0	37.0	41.0
40-41	39.23720112341627	41.0	41.0	41.0	37.0	41.0
42-43	39.11136396983986	41.0	41.0	41.0	37.0	41.0
44-45	39.13387115483829	41.0	41.0	41.0	37.0	41.0
46-47	39.11505149333283	41.0	41.0	41.0	37.0	41.0
48-49	39.14903995550283	41.0	41.0	41.0	37.0	41.0
50-51	39.06137433193926	41.0	41.0	41.0	37.0	41.0
52-53	39.14138945847329	41.0	41.0	41.0	37.0	41.0
54-55	39.01625728809981	41.0	41.0	41.0	37.0	41.0
56-57	39.003851446459365	41.0	41.0	41.0	37.0	41.0
58-59	38.99555468961362	41.0	41.0	41.0	37.0	41.0
60-61	39.013136608409226	41.0	41.0	41.0	37.0	41.0
62-63	39.0270353053578	41.0	41.0	41.0	37.0	41.0
64-65	39.02339052207169	41.0	41.0	41.0	37.0	41.0
66-67	38.970886460305	41.0	41.0	41.0	37.0	41.0
68-69	38.926829292035634	41.0	41.0	41.0	37.0	41.0
70-71	38.881472921454545	41.0	41.0	41.0	34.5	41.0
72-73	38.83798213656676	41.0	41.0	41.0	32.0	41.0
74-75	38.81651671286792	41.0	41.0	41.0	32.0	41.0
76-77	37.99093123583499	41.0	39.0	41.0	32.0	41.0
78-79	38.54772336770098	41.0	39.0	41.0	32.0	41.0
80-81	38.957729343437016	41.0	41.0	41.0	34.5	41.0
82-83	38.9947789331534	41.0	41.0	41.0	37.0	41.0
84-85	38.872628382228314	41.0	41.0	41.0	32.0	41.0
86-87	38.91284089195668	41.0	41.0	41.0	32.0	41.0
88-89	38.79281164444768	41.0	41.0	41.0	32.0	41.0
90-91	38.69770822511799	41.0	41.0	41.0	32.0	41.0
92-93	38.684022650389025	41.0	41.0	41.0	32.0	41.0
94-95	38.509520436988296	41.0	41.0	41.0	32.0	41.0
96-97	38.474629984124434	41.0	37.0	41.0	32.0	41.0
98-99	38.40086810898356	41.0	37.0	41.0	32.0	41.0
100-101	37.29209445351562	41.0	37.0	41.0	29.5	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	42.0
13	155.0
14	468.0
15	1876.0
16	7120.0
17	18889.0
18	32295.0
19	43716.0
20	55233.0
21	67728.0
22	81831.0
23	97348.0
24	115783.0
25	135549.0
26	158697.0
27	185075.0
28	216998.0
29	254612.0
30	299039.0
31	355167.0
32	424236.0
33	514873.0
34	641251.0
35	823641.0
36	1077154.0
37	1507811.0
38	2515775.0
39	6368840.0
40	2.2007505E7
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	33.64655969909051	20.444414776494785	11.56715439074414	34.341871133670566
2	28.866694726933495	25.036431468674486	29.824149375691512	16.272724428700503
3	21.119272942040435	27.482742881751154	28.887954467791644	22.510029708416766
4	25.02283033421715	31.065669223330843	23.072515982023244	20.838984460428765
5	27.753772556562854	32.82428592949386	21.637572749047283	17.784368764896
6	22.466236659944588	37.257180629554426	21.69445882150136	18.582123888999625
7	22.009248671959725	21.73083236732082	36.13994765246198	20.119971308257476
8	22.95573355581597	24.715517508811917	27.201746777791158	25.127002157580954
9	23.606471811340377	23.42118953008429	29.476708385196027	23.49563027337931
10-11	25.000656801466974	30.339724556474827	23.354952417907544	21.304666224150655
12-13	25.630042651979796	25.027385151084758	26.126766787229826	23.21580540970562
14-15	24.63648762734027	26.829495830347426	26.2816266243108	22.2523899180015
16-17	25.55137557313903	26.336232110855985	25.728591737473767	22.383800578531222
18-19	25.062856127766768	26.834792344372893	25.869477259600842	22.232874268259497
20-21	25.28041260980869	26.81577504427855	25.69035022786569	22.213462118047072
22-23	25.248722597355883	26.645552529884448	25.812335463099696	22.293389409659973
24-25	24.765185654743757	26.73513642830749	26.206370810910684	22.293307106038064
26-27	24.983474140214888	26.912536815550332	25.914303183117738	22.189685861117052
28-29	25.205285542952254	26.61500844891	25.832028586158547	22.347677421979206
30-31	24.75710520700791	26.65103506258233	26.21734731518181	22.374512415227947
32-33	24.9113585075606	26.889830225183076	25.989439441827596	22.20937182542873
34-35	25.160243828151867	26.668606759722373	25.816836096139507	22.354313315986246
36-37	24.819084518598338	26.680282994061177	26.219817264829015	22.280815222511468
38-39	24.989267762632352	26.882668616111964	25.96680322624672	22.16126039500896
40-41	25.26562290098854	26.538284199562316	25.865277030274893	22.330815869174256
42-43	24.748936152745365	26.715446009746067	26.258612974708655	22.277004862799906
44-45	24.919022012696068	26.796402035497742	26.066670351216782	22.217905600589408
46-47	25.211115632206315	26.57709635971149	25.937205276446996	22.2745827316352
48-49	24.782144223979863	26.609907236827308	26.28213060729494	22.32581793189789
50-51	25.019938674621656	26.72730967250527	25.95912882519728	22.29362282767579
52-53	25.238282771918552	26.606667119667193	25.915083976891186	22.23996613152307
54-55	24.769209059327057	26.653050432035485	26.295378250330693	22.28236225830677
56-57	24.897690859365994	26.834706995917664	26.08887263379503	22.17872951092132
58-59	25.217454051183985	26.477927939542845	25.984683640290246	22.319934368982924
60-61	24.885951999761733	26.595095024005673	26.218557745418007	22.300395230814587
62-63	24.970899477373376	26.696527511739475	26.140580370608447	22.191992640278702
64-65	25.155940745673977	26.544486420620512	26.076676023602545	22.222896810102966
66-67	24.822685359949055	26.680733454016803	26.318425185671146	22.178156000362993
68-69	25.028963443374924	26.8413740606197	26.071950015375904	22.057712480629473
70-71	25.357052162631966	26.608880921148987	25.860475060770487	22.173591855448553
72-73	24.90618580558938	26.659652141783425	26.32723686056304	22.106925192064157
74-75	24.91452071156449	26.87480537904045	26.12550580398711	22.08516810540795
76-77	25.288744691934646	26.589060286524596	25.951962859530024	22.170232162010738
78-79	24.853279514047635	26.7416286166685	26.315257254387564	22.089834614896304
80-81	25.12451558126289	26.80801317656325	26.1052279692724	21.962243272901464
82-83	25.365791158569213	26.508656615930693	26.027049476970976	22.09850274852912
84-85	25.048001196141968	26.701132761390678	26.2950586674269	21.95580737504045
86-87	25.157114721635736	26.9101636144237	26.029622897305337	21.903098766635228
88-89	25.378976835856555	26.703911568539173	25.911825902317858	22.00528569328641
90-91	25.010095437004555	26.790016752058225	26.246266832634912	21.95362097830231
92-93	25.303401064764934	26.893103209553693	25.959238733094608	21.844256992586768
94-95	25.636850522768757	26.69330377001743	25.800466878384015	21.8693788288298
96-97	25.245173560540273	26.732680287909705	26.152226211686227	21.869919939863795
98-99	25.453277034311355	27.01072435350452	25.84335291411272	21.6926456980714
100-101	25.820806526951767	26.709104827011615	25.74203454989381	21.728054096142806
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	60.0
1	39.5
2	18.0
3	21.0
4	28.0
5	38.5
6	52.5
7	64.5
8	83.5
9	110.0
10	138.0
11	176.5
12	203.0
13	245.0
14	326.5
15	396.5
16	491.5
17	618.0
18	806.0
19	1022.5
20	1338.5
21	1773.0
22	2319.5
23	3184.0
24	4249.5
25	5671.5
26	7859.0
27	10869.5
28	15317.0
29	21351.5
30	29889.5
31	42461.5
32	61262.5
33	90268.0
34	136368.0
35	209909.5
36	316701.5
37	466455.0
38	679660.0
39	955837.0
40	1257499.0
41	1568244.5
42	1891429.0
43	2169643.0
44	2379187.5
45	2507114.0
46	2537246.5
47	2501537.5
48	2404894.0
49	2238653.5
50	2016847.5
51	1804929.5
52	1601356.5
53	1393591.0
54	1206464.0
55	1027798.5
56	840588.0
57	664930.0
58	529424.5
59	428728.0
60	357044.5
61	303506.0
62	256970.5
63	212829.5
64	176626.0
65	146481.0
66	123043.0
67	103343.5
68	80009.5
69	58937.0
70	45829.0
71	36256.5
72	25754.0
73	16688.0
74	9835.0
75	5511.0
76	3099.0
77	1725.0
78	1137.0
79	826.0
80	603.5
81	458.0
82	420.0
83	355.5
84	279.5
85	238.5
86	199.0
87	171.5
88	159.0
89	159.5
90	127.5
91	93.5
92	95.5
93	99.5
94	92.0
95	76.5
96	70.0
97	75.0
98	84.5
99	175.0
100	797.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.007524591667903883
2	0.00635643827610342
3	0.0016390981150713705
4	1.7101344699781552E-4
5	0.014817657438333801
6	0.0016285742106407356
7	0.005138296338257442
8	0.009587276936308304
9	0.003407114059418017
10-11	0.0067076735864758565
12-13	0.004725233089355026
14-15	0.009845112594858856
16-17	1.0787002041400672E-4
18-19	0.013295637760053243
20-21	0.006410373286310424
22-23	0.008851919114217698
24-25	0.011928845672124547
26-27	0.010198978881338952
28-29	0.006895788378173453
30-31	0.011286887501855825
32-33	0.005521103361921783
34-35	0.00510146267275022
36-37	0.009467567523409832
38-39	0.010655453236017737
40-41	0.009117647701091226
42-43	0.0125721193304471
44-45	0.00832046194047064
46-47	0.004451611574158521
48-49	0.0101608297277779
50-51	0.010654137747963907
52-53	0.007536431060388348
54-55	0.010718596662601545
56-57	0.01287468158282785
58-59	0.006017042358215448
60-61	0.002411289602669199
62-63	0.01340219229241342
64-65	0.005164606099334029
66-67	0.006309080706165563
68-69	0.015945030700465553
70-71	0.006289348385358123
72-73	0.00613412079500626
74-75	0.014073091199866388
76-77	0.006605065518277168
78-79	0.013107522968355646
80-81	2.6309761076587E-5
82-83	0.007454870801050928
84-85	0.017177643006903654
86-87	0.002133721623311206
88-89	0.010635720915210298
90-91	0.0037807126667055526
92-93	0.012202467187321053
94-95	0.010389724649144208
96-97	0.004346372529852173
98-99	0.009643842922622966
100-101	0.010721227638709203
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3.8008707E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.76899681400605
#Duplication Level	Percentage of deduplicated	Percentage of total
1	61.63582410338447	19.581082995690586
2	14.922183080627313	9.481255734929283
3	6.958774583718854	6.632198627386515
4	4.057920755527315	5.1566428621534515
5	2.705842053827965	4.29809437936321
6	1.8362542118966325	3.5001572524469586
7	1.2968542903044178	2.8839831871837784
8	0.999493545545588	2.5402325811245916
9	0.7582470293087097	2.1679872712504156
>10	4.388281250315049	25.322534249820798
>50	0.28346420779345055	6.188817074425357
>100	0.1441522658262277	8.392165674435581
>500	0.009139011594571193	1.9892937317072479
>1k	0.0035258398186155423	1.7719345308649055
>5k	4.377051073460941E-5	0.09361984721748053
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.1047808861269603E-5	0.0	7.8929283229761E-6	0.0	0.0
2	3.15717132919044E-5	2.6309761076587005E-5	1.57858566459522E-5	0.0	0.0
3	5.788147436849141E-5	3.6833665507221805E-5	1.57858566459522E-5	0.0	2.6309761076587004E-6
4	1.4207270981356982E-4	3.946464161488051E-5	3.420268939956311E-5	0.0	2.6309761076587004E-6
5	1.6048954256718073E-4	3.946464161488051E-5	4.2095617722539206E-5	0.0	2.6309761076587004E-6
6	1.9732320807440252E-4	3.946464161488051E-5	4.9988546045515305E-5	0.0	2.6309761076587004E-6
7	2.1047808861269604E-4	3.946464161488051E-5	5.525049826083271E-5	0.0	2.6309761076587004E-6
8	2.4731175411991783E-4	3.946464161488051E-5	7.103635490678491E-5	7.8929283229761E-6	5.261952215317401E-6
9	2.6309761076587007E-4	4.735756993785661E-5	7.892928322976102E-5	1.0523904430634802E-5	5.261952215317401E-6
10-11	3.407114059418017E-4	4.9988546045515305E-5	8.419123544507841E-5	1.3154880538293502E-5	1.1839392484464152E-5
12-13	4.012238564179518E-4	4.9988546045515305E-5	1.0129258014485997E-4	1.3154880538293502E-5	1.57858566459522E-5
14-15	4.880460679706889E-4	5.2619522153174E-5	1.2365587705995891E-4	1.3154880538293502E-5	1.8416832753610902E-5
16-17	5.761837675772553E-4	5.788147436849141E-5	1.9074576780525577E-4	1.4470368592122853E-5	2.1047808861269603E-5
18-19	7.182564773908252E-4	5.788147436849141E-5	2.1442455277418407E-4	2.2363296915098955E-5	2.1047808861269603E-5
20-21	8.392813783431254E-4	6.577440269146752E-5	2.525737063352352E-4	2.6309761076587005E-5	2.1047808861269603E-5
22-23	9.958244567488182E-4	6.577440269146752E-5	3.012467643269212E-4	2.7625249130416357E-5	2.1047808861269603E-5
24-25	0.0012562910914070294	6.840537879912621E-5	3.2492554929584947E-4	3.0256225238075054E-5	2.1047808861269603E-5
26-27	0.001629889698694565	7.103635490678491E-5	3.3544945372648426E-4	3.15717132919044E-5	2.1047808861269603E-5
28-29	0.0021087273502884488	7.366733101444361E-5	3.5255079842626583E-4	3.15717132919044E-5	2.3678784968928304E-5
30-31	0.002759893936933977	8.156025933741972E-5	3.630747028569006E-4	3.288720134573376E-5	2.6309761076587005E-5
32-33	0.0035597106736622217	8.419123544507841E-5	3.683366550722181E-4	3.420268939956311E-5	2.8940737184245706E-5
34-35	0.004505546584365525	8.419123544507841E-5	4.3279556970985626E-4	3.420268939956311E-5	3.0256225238075054E-5
36-37	0.005859183791755925	8.419123544507841E-5	4.564743546787845E-4	3.420268939956311E-5	3.5518177453392456E-5
38-39	0.007695605114901699	8.682221155273711E-5	4.69629235217078E-4	3.420268939956311E-5	4.2095617722539206E-5
40-41	0.010105579229517068	9.076867571422516E-5	4.8278411575537153E-4	3.420268939956311E-5	4.2095617722539206E-5
42-43	0.012949664401896124	9.208416376805452E-5	5.196177812625934E-4	3.420268939956311E-5	4.9988546045515305E-5
44-45	0.016514637027773664	9.997709209103061E-5	5.380346140162042E-4	3.420268939956311E-5	5.261952215317401E-5
46-47	0.020513720711414885	1.1970941289847087E-4	5.867076720078902E-4	3.420268939956311E-5	5.788147436849141E-5
48-49	0.0256283382647034	1.3417978149059373E-4	6.051245047615011E-4	3.420268939956311E-5	5.919696242232076E-5
50-51	0.03208738460900551	1.4733466202888722E-4	6.130174330844771E-4	3.420268939956311E-5	6.051245047615011E-5
52-53	0.04007765904796498	1.5391210229803398E-4	6.524820746993576E-4	3.420268939956311E-5	6.182793852997946E-5
54-55	0.04880986874928421	1.6048954256718073E-4	6.708989074529685E-4	3.5518177453392456E-5	6.840537879912621E-5
56-57	0.058978591405385083	1.6312051867483943E-4	6.880002521527501E-4	3.6833665507221805E-5	6.840537879912621E-5
58-59	0.07093900879080153	1.696979589439862E-4	6.998396446372143E-4	3.6833665507221805E-5	6.972086685295556E-5
60-61	0.08598556115050165	1.7232893505164486E-4	7.471972145750709E-4	3.6833665507221805E-5	7.103635490678491E-5
62-63	0.1043195181567213	1.7890637532079161E-4	8.221800336433438E-4	3.6833665507221805E-5	7.103635490678491E-5
64-65	0.12639077672387014	1.8416832753610904E-4	8.682221155273712E-4	3.814915356105115E-5	9.471513987571322E-5
66-67	0.1514389847568348	1.9732320807440252E-4	8.747995557965179E-4	3.946464161488051E-5	1.0129258014485997E-4
68-69	0.18161101875946478	1.9995418418206122E-4	8.958473646577874E-4	3.946464161488051E-5	1.0260806819868932E-4
70-71	0.21822762873780474	2.1047808861269604E-4	8.984783407654462E-4	4.078012966870986E-5	1.0918550846783607E-4
72-73	0.2623517290393488	2.1047808861269604E-4	9.221571257343745E-4	4.2095617722539206E-5	1.1313197262932411E-4
74-75	0.3170602462220038	2.1310906472035474E-4	9.234726137882038E-4	4.3411105776368555E-5	1.1313197262932411E-4
76-77	0.38296225125469274	2.1442455277418407E-4	9.261035898958626E-4	4.4726593830197904E-5	1.1707843679081217E-4
78-79	0.4613416604779531	2.2889492136630692E-4	9.287345660035212E-4	4.8673057991685957E-5	1.1839392484464152E-4
80-81	0.554641335207746	2.3678784968928304E-4	9.339965182188387E-4	5.261952215317401E-5	1.1839392484464152E-4
82-83	0.6672563210319151	2.4468077801225916E-4	9.445204226494735E-4	5.261952215317401E-5	1.1970941289847087E-4
84-85	0.8050944748002082	2.4731175411991783E-4	9.524133509724495E-4	5.261952215317401E-5	1.2234038900612958E-4
86-87	0.9736163874240711	2.5520468244289395E-4	9.642527434569137E-4	5.261952215317401E-5	1.2497136511378825E-4
88-89	1.1724642461528618	2.6835956298118746E-4	9.708301837260604E-4	5.261952215317401E-5	1.2760234122144697E-4
90	1.3415136694863101	2.7888346741182225E-4	9.760921359413779E-4	5.261952215317401E-5	1.3154880538293503E-4
>>END_MODULE
