##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	SRR19654111_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	27254776
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.437331607495143	32.0	32.0	32.0	32.0	32.0
2	31.312094144527183	32.0	32.0	32.0	32.0	32.0
3	35.15013082477728	37.0	32.0	37.0	32.0	37.0
4	36.00552750827965	37.0	37.0	37.0	32.0	37.0
5	36.16563390577857	37.0	37.0	37.0	37.0	37.0
6	39.30723543646075	41.0	41.0	41.0	37.0	41.0
7	39.33950669783527	41.0	41.0	41.0	37.0	41.0
8	39.56056259644181	41.0	41.0	41.0	37.0	41.0
9	39.745985327489024	41.0	41.0	41.0	37.0	41.0
10-11	39.78878382636496	41.0	41.0	41.0	37.0	41.0
12-13	39.69901313810101	41.0	41.0	41.0	37.0	41.0
14-15	39.64046017109075	41.0	41.0	41.0	37.0	41.0
16-17	39.66986250409836	41.0	41.0	41.0	37.0	41.0
18-19	39.62535318580494	41.0	41.0	41.0	37.0	41.0
20-21	39.62369987924318	41.0	41.0	41.0	37.0	41.0
22-23	39.622453198661404	41.0	41.0	41.0	37.0	41.0
24-25	39.580426858030314	41.0	41.0	41.0	37.0	41.0
26-27	39.43598310989604	41.0	41.0	41.0	37.0	41.0
28-29	39.48254595818362	41.0	41.0	41.0	37.0	41.0
30-31	39.54498044672978	41.0	41.0	41.0	37.0	41.0
32-33	39.52960044507429	41.0	41.0	41.0	37.0	41.0
34-35	39.40138166976679	41.0	41.0	41.0	37.0	41.0
36-37	39.320839877752064	41.0	41.0	41.0	37.0	41.0
38-39	39.2711742338297	41.0	41.0	41.0	37.0	41.0
40-41	39.26842737581113	41.0	41.0	41.0	37.0	41.0
42-43	39.227835902962475	41.0	41.0	41.0	37.0	41.0
44-45	39.218544155343636	41.0	41.0	41.0	37.0	41.0
46-47	39.1703211209661	41.0	41.0	41.0	37.0	41.0
48-49	39.14010432520158	41.0	41.0	41.0	37.0	41.0
50-51	39.14161741780596	41.0	41.0	41.0	37.0	41.0
52-53	39.15251391169019	41.0	41.0	41.0	37.0	41.0
54-55	39.14942061897702	41.0	41.0	41.0	37.0	41.0
56-57	39.107406899987	41.0	41.0	41.0	37.0	41.0
58-59	39.07522910846892	41.0	41.0	41.0	37.0	41.0
60-61	39.02996590395753	41.0	41.0	41.0	37.0	41.0
62-63	38.96906822862899	41.0	41.0	41.0	37.0	41.0
64-65	38.98838979634248	41.0	41.0	41.0	37.0	41.0
66-67	38.97820077479265	41.0	41.0	41.0	37.0	41.0
68-69	38.92085172521689	41.0	41.0	41.0	37.0	41.0
70-71	38.84381350263161	41.0	41.0	41.0	32.0	41.0
72-73	38.855061109289615	41.0	41.0	41.0	32.0	41.0
74-75	38.75617770624862	41.0	41.0	41.0	32.0	41.0
76-77	37.84043148987906	41.0	39.0	41.0	29.5	41.0
78-79	38.38136440747119	41.0	39.0	41.0	32.0	41.0
80-81	38.850647717669744	41.0	41.0	41.0	32.0	41.0
82-83	38.926867661653134	41.0	41.0	41.0	34.5	41.0
84-85	38.895665405578825	41.0	41.0	41.0	32.0	41.0
86-87	38.8500576192591	41.0	41.0	41.0	32.0	41.0
88-89	38.77521884604739	41.0	41.0	41.0	32.0	41.0
90-91	38.660068752720626	41.0	41.0	41.0	32.0	41.0
92-93	38.579713533510606	41.0	41.0	41.0	32.0	41.0
94-95	38.4455774283377	41.0	41.0	41.0	32.0	41.0
96-97	38.47975776795964	41.0	41.0	41.0	32.0	41.0
98-99	38.42640165158576	41.0	37.0	41.0	32.0	41.0
100-101	37.3817250598574	41.0	37.0	41.0	29.5	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	27.0
13	108.0
14	407.0
15	1694.0
16	6712.0
17	17255.0
18	28683.0
19	38033.0
20	45488.0
21	54185.0
22	63568.0
23	75059.0
24	87852.0
25	101544.0
26	117463.0
27	135862.0
28	158224.0
29	184506.0
30	216673.0
31	257318.0
32	303249.0
33	361812.0
34	443026.0
35	560080.0
36	736814.0
37	1048396.0
38	1748816.0
39	4417315.0
40	1.6044607E7
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	33.55465811209755	19.36483006843413	14.006466569044646	33.07404525042368
2	29.94805537530949	22.508199870927346	30.282280749314168	17.261464004449
3	22.24318751047072	25.842201752997497	28.78743215725918	23.127178579272602
4	24.975973545197384	31.060961939519853	22.992619191964817	20.970445323317946
5	27.129056639772227	32.699893963243035	22.039387701937645	18.131661695047093
6	21.761971541745094	36.3127859464549	22.238484603998753	19.686757907801248
7	21.447000701821946	19.856637236717702	37.66652494227067	21.02983711918968
8	23.115889872992934	24.187646853478434	26.62837258380796	26.068090689720673
9	23.325864061403404	23.46625046560647	29.014437689746558	24.193447783243567
10-11	25.413503673631364	29.5248326385071	22.859340322591535	22.20232336527
12-13	25.847045189790453	24.099966700002135	25.98586825951381	24.067119850693604
14-15	24.401126980460234	26.670364122603686	26.53859822586691	22.389910671069174
16-17	25.341367216099798	26.191821753223763	25.89584293916841	22.57096809150802
18-19	24.90032939547916	26.821247769565232	25.95467304519399	22.323749789761617
20-21	25.170743577283254	26.751291993444575	25.74645192658708	22.33151250268509
22-23	25.162321559475753	26.56681859539876	25.891076442404614	22.37978340272087
24-25	24.67297377035382	26.644008493045124	26.270793102609446	22.412224633991602
26-27	24.95293461515197	26.739403887810397	25.95691726907516	22.35074422796248
28-29	25.173230981576733	26.49415379261859	25.91868016544933	22.41393506035534
30-31	24.735694530560988	26.570983102274383	26.233373851066528	22.459948516098102
32-33	24.84104953947382	26.825740989896417	26.01542023698387	22.31778923364589
34-35	25.105199910650523	26.604249104817445	25.89623741541666	22.394313569115372
36-37	24.779060741500867	26.52947872328872	26.23531560119958	22.456144934010833
38-39	24.985824830142416	26.720659783320006	25.94394468132969	22.34957070520789
40-41	25.21212428970247	26.458126091368356	25.875921710015152	22.453827908914022
42-43	24.767265801974595	26.62623713983366	26.21817800166617	22.388319056525578
44-45	24.93560182704602	26.63680516522422	26.08195076389323	22.345642243836533
46-47	25.176479160936783	26.468823299079762	25.95962997457767	22.395067565405785
48-49	24.771833004456532	26.509672940642936	26.25254362784991	22.465950427050625
50-51	25.05398792209375	26.5887542029565	25.946855957657306	22.410401917292447
52-53	25.225862244481533	26.50608233788555	25.94968289860536	22.31837251902756
54-55	24.746838537395117	26.587467781805945	26.278174953450506	22.387518727348425
56-57	24.82337862152488	26.709153892458694	26.16590841832705	22.301559067689375
58-59	25.182261397986476	26.385003077736712	26.03857722321462	22.39415830106218
60-61	24.907425399496955	26.506968907027524	26.202244333250068	22.38336136022545
62-63	25.00652852765694	26.648754571207732	26.056218906476662	22.28849799465866
64-65	25.17215560994072	26.49431473027543	26.079347085430953	22.25418257435289
66-67	24.830515408690804	26.599506129353014	26.347217482874136	22.222760979082047
68-69	25.026683945898693	26.772917762923203	26.073335248967393	22.12706304221071
70-71	25.35405977133055	26.587499564984174	25.81706153995768	22.2413791237276
72-73	24.899366091683902	26.61826772820718	26.290546631722272	22.191819548386647
74-75	24.893392323733636	26.832971045919436	26.096341374103254	22.177295256243678
76-77	25.260248031594436	26.592968112293992	25.95691755318437	22.189866302927207
78-79	24.9119331165916	26.697802323095587	26.246010712679897	22.144253847632914
80-81	25.140191428587467	26.74958091997161	26.079803213733914	22.030424437707005
82-83	25.37361760426918	26.480079075735468	26.033240700740585	22.113062619254762
84-85	25.086785701961468	26.639600565533193	26.233790669025144	22.039823063480195
86-87	25.16353647522181	26.87770576430347	25.962361605907162	21.996396154567552
88-89	25.411579240277003	26.693807720158848	25.878466218177685	22.016146821386464
90-91	25.03823366590868	26.774169782206247	26.151480753318246	22.036115798566826
92-93	25.333948809559104	26.8792834694367	25.875086993927233	21.91168072707697
94-95	25.65367743952296	26.74794664345849	25.75723547790634	21.841140439112205
96-97	25.33721809359934	26.775502820646373	26.07663124431151	21.81064784144278
98-99	25.589926902717046	26.99173997950818	25.766617042334783	21.651716075439992
100-101	25.909998093902892	26.769655227576806	25.677146042445244	21.64320063607506
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	44.0
1	38.5
2	22.5
3	17.5
4	21.5
5	24.5
6	32.5
7	40.5
8	52.5
9	81.5
10	115.0
11	134.0
12	150.0
13	195.0
14	273.5
15	336.5
16	393.5
17	516.5
18	674.0
19	879.5
20	1163.0
21	1570.0
22	2143.5
23	2790.0
24	3738.0
25	5077.5
26	6857.5
27	9390.0
28	12968.5
29	17850.0
30	24565.0
31	34085.5
32	48504.5
33	69683.5
34	102297.5
35	153596.0
36	227981.5
37	332494.5
38	478293.0
39	667436.5
40	877623.5
41	1092781.5
42	1318009.0
43	1512682.0
44	1656195.0
45	1749871.0
46	1786768.5
47	1780301.5
48	1715133.5
49	1597915.0
50	1448516.5
51	1299749.0
52	1160512.5
53	1021089.5
54	886611.5
55	754164.0
56	623558.5
57	501391.5
58	399179.5
59	323833.0
60	275695.0
61	237211.0
62	199302.0
63	164376.5
64	139482.5
65	119578.5
66	100544.5
67	83016.5
68	63633.5
69	46910.0
70	36659.5
71	27950.0
72	18662.0
73	11700.5
74	6325.0
75	3044.5
76	1743.0
77	1054.5
78	749.5
79	566.5
80	473.5
81	396.0
82	317.5
83	266.5
84	233.5
85	207.0
86	173.0
87	149.5
88	122.5
89	101.5
90	95.5
91	84.0
92	67.5
93	53.0
94	55.5
95	56.0
96	52.5
97	54.0
98	66.5
99	133.0
100	557.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.017527203305578443
2	0.007000607893456912
3	7.411545044435514E-4
4	0.0016254031953885806
5	2.7885020959262334E-4
6	0.0012071278809996457
7	0.0
8	0.023951765371324277
9	0.0
10-11	0.0
12-13	0.003744297880122001
14-15	0.0
16-17	0.001069537317055917
18-19	0.0
20-21	3.3205189431753174E-4
22-23	5.503622557749144E-6
24-25	1.8345408525830482E-5
26-27	0.005380708320626081
28-29	9.869829786896798E-4
30-31	1.5777051332214215E-4
32-33	5.008296527551721E-4
34-35	0.0
36-37	0.0
38-39	9.264431305544393E-4
40-41	0.0
42-43	9.356158348173546E-5
44-45	0.0018675625879295433
46-47	0.0
48-49	0.0014529563552457742
50-51	0.001102559052402412
52-53	6.072330222049889E-4
54-55	6.732764928979787E-4
56-57	0.008332484552432204
58-59	6.255784307308194E-4
60-61	0.0
62-63	0.004810166115472752
64-65	1.834540852583048E-6
66-67	0.0024802992326922813
68-69	3.393900577278639E-4
70-71	7.521617495590497E-5
72-73	1.6877775843764044E-4
74-75	6.310820532885686E-4
76-77	3.7791541563210793E-4
78-79	2.3665576998321323E-4
80-81	0.0015960505417472518
82-83	0.005171570663431613
84-85	5.228441429861688E-4
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	4.035989875682706E-5
96-97	0.0046634028472661085
98-99	3.669081705166096E-6
100-101	4.806497033767587E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2.7254776E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.90209638576306
#Duplication Level	Percentage of deduplicated	Percentage of total
1	63.61395303113048	22.2026032017192
2	15.156675144651432	10.579994735726471
3	6.792842973148818	7.1125338054658
4	3.8595807504911894	5.3882983744911686
5	2.356661482622436	4.112621310755177
6	1.5805727811115837	3.3099182130642038
7	1.115630420077317	2.7256488316699494
8	0.8486853443904294	2.369671815287943
9	0.6703292038401254	2.1056305034357883
>10	3.631600898058175	22.956038716816902
>50	0.23344638529272055	5.600486930225208
>100	0.13005867186791906	8.436409915717377
>500	0.007596154244171504	1.7989992587252899
>1k	0.002355632511213956	1.2761935204688357
>5k	1.1126561833390648E-5	0.024950866430661574
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.5683571936162673E-5	0.0	1.1007245115498289E-5	0.0	0.0
2	3.3021735346494865E-5	1.1007245115498289E-5	1.4676326820664386E-5	0.0	0.0
3	7.338163410332193E-5	1.1007245115498289E-5	1.8345408525830482E-5	0.0	7.338163410332193E-6
4	1.3575602309114557E-4	1.1007245115498289E-5	3.6690817051660964E-5	0.0	7.338163410332193E-6
5	1.5043234991180996E-4	1.1007245115498289E-5	4.4028980461993155E-5	1.1007245115498289E-5	1.4676326820664386E-5
6	1.834540852583048E-4	1.1007245115498289E-5	5.5036225577491446E-5	1.1007245115498289E-5	1.4676326820664386E-5
7	2.2014490230996578E-4	1.1007245115498289E-5	7.338163410332193E-5	1.8345408525830482E-5	1.8345408525830482E-5
8	2.4582847424612844E-4	1.1007245115498289E-5	9.539612433431851E-5	2.2014490230996578E-5	1.8345408525830482E-5
9	2.6050480106679287E-4	1.4676326820664386E-5	9.90652060394846E-5	2.2014490230996578E-5	2.2014490230996578E-5
10-11	3.04533781528786E-4	1.4676326820664386E-5	1.064033694498168E-4	2.2014490230996578E-5	3.6690817051660964E-5
12-13	3.724117930743588E-4	1.4676326820664386E-5	1.2474877797564727E-4	2.2014490230996578E-5	3.6690817051660964E-5
14-15	4.5496613144059594E-4	1.8345408525830482E-5	1.5777051332214215E-4	2.5683571936162673E-5	3.6690817051660964E-5
16-17	5.68707664300745E-4	2.5683571936162673E-5	2.550011785090437E-4	2.9352653641328773E-5	3.6690817051660964E-5
18-19	7.301472593280532E-4	2.5683571936162673E-5	2.843538321503725E-4	2.9352653641328773E-5	3.852535790424402E-5
20-21	9.191049671441071E-4	2.9352653641328773E-5	3.192101083494504E-4	2.9352653641328773E-5	4.035989875682706E-5
22-23	0.0011575952779799034	3.3021735346494865E-5	3.5590092540111135E-4	3.118719449391182E-5	4.035989875682706E-5
24-25	0.0014933162540026012	3.485627619907791E-5	3.669081705166096E-4	3.3021735346494865E-5	4.035989875682706E-5
26-27	0.0020363403463671834	3.6690817051660964E-5	3.8158449733727404E-4	3.3021735346494865E-5	4.4028980461993155E-5
28-29	0.002733465870348742	3.6690817051660964E-5	4.054335284208537E-4	3.3021735346494865E-5	5.687076643007449E-5
30-31	0.0035535056314533646	4.58635213145762E-5	4.146062326837689E-4	3.485627619907791E-5	5.8705307282657545E-5
32-33	0.004534984987585295	5.687076643007449E-5	4.146062326837689E-4	3.852535790424402E-5	5.8705307282657545E-5
34-35	0.005672400316186784	6.237438898782364E-5	4.95326030197423E-4	4.035989875682706E-5	5.8705307282657545E-5
36-37	0.0070061115160146605	6.604347069298974E-5	5.100023570180875E-4	4.035989875682706E-5	6.053984813524059E-5
38-39	0.008787450683872801	6.971255239815584E-5	5.173405204284196E-4	4.219443960941011E-5	6.237438898782364E-5
40-41	0.011298937111058994	6.971255239815584E-5	5.411895515119991E-4	4.4028980461993155E-5	6.237438898782364E-5
42-43	0.014529563552457742	7.338163410332193E-5	6.017293996472398E-4	5.136714387232535E-5	7.338163410332193E-5
44-45	0.018273861432579745	8.071979751365413E-5	6.237438898782364E-4	5.136714387232535E-5	7.338163410332193E-5
46-47	0.02210621727362573	8.989250177656935E-5	6.769455746031448E-4	5.32016847249084E-5	7.521617495590498E-5
48-49	0.026949405124444978	9.539612433431851E-5	6.971255239815583E-4	5.5036225577491446E-5	7.705071580848802E-5
50-51	0.033484039641345796	1.0089974689206765E-4	7.081327690970566E-4	5.5036225577491446E-5	7.705071580848802E-5
52-53	0.0420330000143828	1.0823791030239985E-4	7.466581270013006E-4	5.5036225577491446E-5	7.705071580848802E-5
54-55	0.051730382961136794	1.1374153286014898E-4	7.888525666107107E-4	5.5036225577491446E-5	7.705071580848802E-5
56-57	0.06241841796828563	1.1741061456531509E-4	8.512269555985344E-4	5.8705307282657545E-5	7.705071580848802E-5
58-59	0.07463829458734131	1.1741061456531509E-4	8.860832317976123E-4	6.420892984040669E-5	7.705071580848802E-5
60-61	0.09003192688136567	1.1741061456531509E-4	9.53961243343185E-4	6.604347069298973E-5	7.705071580848802E-5
62-63	0.10961014686013196	1.1741061456531509E-4	0.0010860481847291645	6.604347069298973E-5	7.705071580848802E-5
64-65	0.1342058360707129	1.2291423712306425E-4	0.001139249869454073	6.787801154557278E-5	8.255433836623717E-5
66-67	0.16298427842518315	1.339214822385625E-4	0.001144753492011822	6.971255239815584E-5	8.438887921882022E-5
68-69	0.1969324569022325	1.3942510479631167E-4	0.0011612643596850694	7.154709325073887E-5	8.438887921882022E-5
70-71	0.2380445174086186	1.4309418650147775E-4	0.001175940686505734	7.338163410332193E-5	8.805796092398631E-5
72-73	0.2899124909336991	1.4676326820664386E-4	0.0012071278809996457	7.338163410332193E-5	9.539612433431851E-5
74-75	0.3553157068691374	1.485978090592269E-4	0.0012144660444099778	7.338163410332193E-5	9.723066518690156E-5
76-77	0.4353108607460212	1.52266890764393E-4	0.001218135126115144	7.338163410332193E-5	1.0089974689206764E-4
78-79	0.533310198550155	1.559359724695591E-4	0.0012218042078203102	7.338163410332193E-5	1.027342877446507E-4
80-81	0.6501961344316314	1.5777051332214215E-4	0.0012346459937883913	7.521617495590498E-5	1.1007245115498289E-4
82-83	0.7924445976000684	1.5777051332214215E-4	0.0012731713516926355	7.705071580848802E-5	1.1190699200756593E-4
84-85	0.9675368456522996	1.596050541747252E-4	0.0012860131376607167	7.705071580848802E-5	1.1374153286014898E-4
86-87	1.1867094413103962	1.6510867673247433E-4	0.0013116967095968795	8.255433836623717E-5	1.1374153286014898E-4
88-89	1.4499091828896338	1.7978500355313873E-4	0.0013172003321546286	8.805796092398631E-5	1.1374153286014898E-4
90	1.6758493997529094	1.834540852583048E-4	0.0013245384955649607	9.539612433431851E-5	1.1374153286014898E-4
>>END_MODULE
