##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	SRR19654126_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	29541717
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.113156760658157	32.0	32.0	32.0	32.0	32.0
2	31.257886906167304	32.0	32.0	32.0	32.0	32.0
3	35.031830546613115	37.0	32.0	37.0	32.0	37.0
4	35.718425371145486	37.0	37.0	37.0	32.0	37.0
5	35.83022212283734	37.0	37.0	37.0	32.0	37.0
6	39.1534473097823	41.0	41.0	41.0	37.0	41.0
7	39.36710503319763	41.0	41.0	41.0	37.0	41.0
8	39.346778794204816	41.0	41.0	41.0	37.0	41.0
9	39.360474375947746	41.0	41.0	41.0	37.0	41.0
10-11	39.44684581129797	41.0	41.0	41.0	37.0	41.0
12-13	39.44746216003627	41.0	41.0	41.0	37.0	41.0
14-15	39.429059810572284	41.0	41.0	41.0	37.0	41.0
16-17	39.24205116446008	41.0	41.0	41.0	37.0	41.0
18-19	39.093552212960404	41.0	41.0	41.0	37.0	41.0
20-21	38.95528829620838	41.0	41.0	41.0	37.0	41.0
22-23	38.843539189005156	41.0	41.0	41.0	34.5	41.0
24-25	38.83921582824722	41.0	41.0	41.0	34.5	41.0
26-27	38.785608653010925	41.0	41.0	41.0	32.0	41.0
28-29	38.91412635223606	41.0	41.0	41.0	37.0	41.0
30-31	39.04058284763882	41.0	41.0	41.0	37.0	41.0
32-33	39.09686942028454	41.0	41.0	41.0	37.0	41.0
34-35	39.04859465345227	41.0	41.0	41.0	37.0	41.0
36-37	38.99805923602884	41.0	41.0	41.0	37.0	41.0
38-39	39.068393519577754	41.0	41.0	41.0	37.0	41.0
40-41	39.03288398910598	41.0	41.0	41.0	37.0	41.0
42-43	38.921287039612494	41.0	41.0	41.0	37.0	41.0
44-45	38.92831835062262	41.0	41.0	41.0	37.0	41.0
46-47	38.89640918637194	41.0	41.0	41.0	34.5	41.0
48-49	38.92659766526096	41.0	41.0	41.0	32.0	41.0
50-51	38.829257503888485	41.0	41.0	41.0	32.0	41.0
52-53	38.89761564976064	41.0	41.0	41.0	32.0	41.0
54-55	38.77114348160603	41.0	41.0	41.0	32.0	41.0
56-57	38.75000716782982	41.0	41.0	41.0	32.0	41.0
58-59	38.728644123833426	41.0	41.0	41.0	32.0	41.0
60-61	38.73635083566741	41.0	41.0	41.0	32.0	41.0
62-63	38.736814434313345	41.0	41.0	41.0	32.0	41.0
64-65	38.7166643868398	41.0	41.0	41.0	32.0	41.0
66-67	38.65770965512939	41.0	41.0	41.0	32.0	41.0
68-69	38.59039405190971	41.0	41.0	41.0	32.0	41.0
70-71	38.527920127323675	41.0	41.0	41.0	32.0	41.0
72-73	38.48150170147524	41.0	41.0	41.0	32.0	41.0
74-75	38.447948049194295	41.0	41.0	41.0	32.0	41.0
76-77	37.73663832742017	41.0	39.0	41.0	29.5	41.0
78-79	38.29144622839627	41.0	39.0	41.0	32.0	41.0
80-81	38.67820001796104	41.0	41.0	41.0	32.0	41.0
82-83	38.704009452124936	41.0	41.0	41.0	32.0	41.0
84-85	38.57472981343636	41.0	41.0	41.0	32.0	41.0
86-87	38.60964146058267	41.0	41.0	41.0	32.0	41.0
88-89	38.48130152692208	41.0	41.0	41.0	32.0	41.0
90-91	38.38251559650375	41.0	39.0	41.0	32.0	41.0
92-93	38.35002720051783	41.0	37.0	41.0	32.0	41.0
94-95	38.16619025563071	41.0	37.0	41.0	32.0	41.0
96-97	38.12742698740225	41.0	37.0	41.0	32.0	41.0
98-99	38.03774579182381	41.0	37.0	41.0	32.0	41.0
100-101	36.93800573609178	41.0	37.0	41.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	50.0
13	222.0
14	709.0
15	1678.0
16	5596.0
17	14700.0
18	27009.0
19	39268.0
20	51389.0
21	65439.0
22	80640.0
23	97709.0
24	116643.0
25	137461.0
26	160343.0
27	188193.0
28	217864.0
29	254665.0
30	301600.0
31	361908.0
32	413311.0
33	489140.0
34	597150.0
35	751893.0
36	977021.0
37	1344235.0
38	2167893.0
39	5051958.0
40	1.5626029E7
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	27.48659391883916	21.652848452234615	12.027098864218368	38.83345876470786
2	27.582507172406235	25.12313241455714	31.424240537736914	15.870119875299709
3	20.56404914895187	28.246308040087715	28.753805699290684	22.43583711166973
4	24.28757770631716	30.74728524222625	23.637140720307087	21.327996331149503
5	28.121542116855885	32.216715113424996	22.072563155139996	17.589179614579123
6	22.59632077367032	37.29345036510793	21.894556648132703	18.215672213089046
7	22.334362549834513	22.66364186921785	35.166598003335785	19.83539757761185
8	22.519087897527847	24.547459053449362	28.33054503025403	24.60290801876876
9	23.565869645202632	23.105780189871105	30.15333686043532	23.17501330449094
10-11	24.895402507238924	30.222885335440232	23.609186329399716	21.272525827921125
12-13	25.66844470839967	25.330793147978813	25.84535011146105	23.15541203216047
14-15	24.91711364446773	27.009226702421508	25.769962489174038	22.30369716393673
16-17	25.955321611197736	26.451349470134367	25.228643421266984	22.36468549740092
18-19	25.387337726375975	27.20962763861691	25.21020438881305	22.192830246194063
20-21	25.78535099718242	27.145444492481197	25.077668728591018	21.991535781745362
22-23	25.526414543423343	26.91163668524372	25.403369260260757	22.15857951107218
24-25	24.90711669072726	27.108888984621558	25.798473827472478	22.185520497178704
26-27	25.303384818715585	27.12595685573604	25.609703913208403	21.960954412339973
28-29	25.418511585973636	26.71286192499554	25.53816080090769	22.330465688123134
30-31	25.11632908725429	26.817117514048995	25.749741274156833	22.31681212453988
32-33	25.365455927457848	27.088290750598297	25.431799595001664	22.114453726942184
34-35	25.49927368617359	26.834160334602323	25.370859113262405	22.29570686596168
36-37	25.14206316035215	26.87080465286303	25.728994514162885	22.258137672621938
38-39	25.458051802326004	27.048419806805562	25.44348782535144	22.050040565516994
40-41	25.633559582779487	26.665506962852337	25.420882519255844	22.280050935112335
42-43	25.096602890260368	26.873674242478746	25.821758706574744	22.20796416068615
44-45	25.28552702443451	26.888268563230106	25.70020815981463	22.12599625252075
46-47	25.535546084175074	26.700116839771088	25.52441727793292	22.239919798120916
48-49	25.141698869102118	26.757124120759805	25.79778711619677	22.303389893941304
50-51	25.442530817946047	26.847057793969025	25.510985332154874	22.19942605593005
52-53	25.622684325659062	26.6595048647685	25.463450546269495	22.254360263302942
54-55	25.110379195675574	26.83893781390253	25.824511889990287	22.22617110043161
56-57	25.28567477239457	27.024276558029058	25.62726572857435	22.06278294100202
58-59	25.521521559609706	26.711182793937144	25.522516820071058	22.244778826382095
60-61	25.21825958829631	26.833981993348065	25.699011315703217	22.248747102652406
62-63	25.377989738947292	26.885220871283867	25.681484519636715	22.055304870132122
64-65	25.495757066292164	26.72303487447298	25.62592217635549	22.155285882879365
66-67	25.143076294227797	26.942486966324537	25.81706251318237	22.09737422626529
68-69	25.422150621163354	27.076585926165613	25.562709360519015	21.938554092152017
70-71	25.685647129013816	26.79322997628933	25.388922705711575	22.13220018898528
72-73	25.232838159996145	26.906860250556935	25.816612851205022	22.043688738241894
74-75	25.311121421111704	27.054744410390747	25.65543944165659	21.97869472684096
76-77	25.63030130802589	26.7357193318084	25.55051409334055	22.08346526682517
78-79	25.17189968514899	26.91462618778808	25.892103949904833	22.021370177158094
80-81	25.527053430597153	26.968804502907084	25.625460038026564	21.8786820284692
82-83	25.668026969677115	26.649476646102364	25.62934163725433	22.05315474696619
84-85	25.3479080269623	26.85980399009416	25.889515192131256	21.90277279081228
86-87	25.524984339189455	26.99642096888384	25.647747220477996	21.830847471448713
88-89	25.599216540095775	26.808986436495978	25.57303226089732	22.018764762510923
90-91	25.218259467134825	26.99826980746492	25.85886332941365	21.924607395986605
92-93	25.561775951879838	27.08512426856844	25.608127760840304	21.744972018711415
94-95	25.842283725143712	26.807503836244926	25.533317347498368	21.816895091112993
96-97	25.41331933852386	26.935644164975542	25.81989707951896	21.831139416981635
98-99	25.648481546426456	27.20769822451814	25.52315895136076	21.620661277694648
100-101	25.92771241370934	26.87685652127364	25.52716042434288	21.668270640674134
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	40.0
1	27.0
2	24.0
3	32.0
4	34.5
5	49.0
6	61.5
7	76.0
8	91.0
9	116.0
10	138.5
11	162.5
12	200.5
13	261.0
14	336.0
15	428.5
16	535.0
17	690.5
18	939.0
19	1183.5
20	1437.0
21	1831.5
22	2393.0
23	3154.0
24	4229.5
25	5690.5
26	7612.5
27	10150.5
28	13999.0
29	19058.0
30	25775.5
31	35375.5
32	49360.5
33	69958.0
34	101360.0
35	150510.5
36	221414.0
37	321971.0
38	469739.0
39	667065.0
40	896876.0
41	1148259.0
42	1418594.5
43	1664805.5
44	1856608.0
45	1972309.5
46	2006734.5
47	1988646.5
48	1912142.0
49	1772531.0
50	1593649.5
51	1427684.5
52	1271308.5
53	1113003.5
54	963732.0
55	818931.5
56	669061.5
57	527544.5
58	418803.0
59	338772.5
60	281998.0
61	240461.0
62	203689.0
63	167748.0
64	142854.0
65	123247.5
66	104203.5
67	85114.0
68	63241.0
69	46584.0
70	37188.5
71	28653.5
72	19763.0
73	12937.0
74	6979.0
75	3269.0
76	1643.0
77	727.5
78	435.5
79	334.5
80	260.0
81	200.0
82	171.0
83	147.0
84	122.5
85	106.5
86	91.5
87	78.5
88	75.5
89	76.0
90	62.0
91	47.5
92	44.5
93	44.5
94	44.5
95	44.5
96	49.5
97	53.5
98	67.5
99	147.0
100	666.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.00786684132137614
2	0.005991527168173739
3	0.0016823666681256206
4	1.3201670031569255E-4
5	0.014003925364256925
6	0.0015300397062228983
7	0.004898158086071977
8	0.009623678948654204
9	0.0031819409819679747
10-11	0.0065382117092246195
12-13	0.0045799639878751795
14-15	0.009403651114794716
16-17	9.985878613622897E-5
18-19	0.012859780628187588
20-21	0.006214940045631065
22-23	0.008774033005596798
24-25	0.011659782672753922
26-27	0.009857246956905043
28-29	0.006881793634405204
30-31	0.010867682470859768
32-33	0.005400837060350961
34-35	0.0049286234784525216
36-37	0.009285174588870376
38-39	0.01043270436853755
40-41	0.008684329350254084
42-43	0.012360486697506444
44-45	0.00810379437322482
46-47	0.004271925020471897
48-49	0.00992325530706289
50-51	0.010576568721445677
52-53	0.007211835385194436
54-55	0.010456399673722418
56-57	0.012717608797078383
58-59	0.005791809595901281
60-61	0.0023339875607094876
62-63	0.012903786194959488
64-65	0.005223122271464451
66-67	0.006077845779918615
68-69	0.015569508028257125
70-71	0.006262330656000801
72-73	0.0058375076844720975
74-75	0.01371958170203851
76-77	0.0064197351833002805
78-79	0.012787002190834068
80-81	5.0775653967574056E-6
82-83	0.007100128946465772
84-85	0.01690998529300108
86-87	0.002103804596056485
88-89	0.01015682331531373
90-91	0.0036355368240783025
92-93	0.011832419896243675
94-95	0.010378543670972138
96-97	0.004200839104917294
98-99	0.009308869894055244
100-101	0.010508867849488911
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2.9541717E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.022283625774755
#Duplication Level	Percentage of deduplicated	Percentage of total
1	63.83219277563739	21.71716967067865
2	14.69520811601679	9.999290769258206
3	6.776223708352089	6.916278147517618
4	3.698414498112057	5.033140280817832
5	2.427627829120578	4.129672127008208
6	1.6411230610473886	3.350085254865234
7	1.1694661277490108	2.7851535802309364
8	0.8784671234755577	2.3909966104643128
9	0.6587152651219964	2.0169897820747176
>10	3.817707526910605	23.61833013489129
>50	0.2607733176106434	6.103239108850713
>100	0.13287633757612727	8.216890144481898
>500	0.008141468448674615	1.8763678055388484
>1k	0.0029903230242315725	1.6326987810681517
>5k	6.216154022399094E-5	0.12481302353402382
>10k+	1.036025670399849E-5	0.08888477871949954
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.6925217989191352E-5	0.0	6.770087195676541E-6	1.6925217989191352E-5	0.0
2	2.3695305184867892E-5	2.7080348782706165E-5	2.3695305184867892E-5	1.6925217989191352E-5	0.0
3	4.0620523174059245E-5	3.0465392380544435E-5	2.3695305184867892E-5	1.6925217989191352E-5	1.3540174391353083E-5
4	6.431582835892714E-5	4.400556677189752E-5	2.3695305184867892E-5	1.6925217989191352E-5	1.3540174391353083E-5
5	8.124104634811849E-5	4.400556677189752E-5	2.3695305184867892E-5	1.6925217989191352E-5	1.3540174391353083E-5
6	1.3540174391353082E-4	5.077565396757406E-5	2.7080348782706165E-5	1.6925217989191352E-5	1.3540174391353083E-5
7	1.4555687470704565E-4	5.077565396757406E-5	3.3850435978382705E-5	1.6925217989191352E-5	1.3540174391353083E-5
8	1.5571200550056045E-4	5.077565396757406E-5	3.3850435978382705E-5	1.6925217989191352E-5	1.6925217989191352E-5
9	1.6586713629407524E-4	5.077565396757406E-5	4.7390610369735784E-5	1.6925217989191352E-5	1.6925217989191352E-5
10-11	2.098727030659728E-4	5.24681757664932E-5	5.75457411632506E-5	1.6925217989191352E-5	2.200278338594876E-5
12-13	2.4203061724543636E-4	5.75457411632506E-5	7.785600275028022E-5	1.6925217989191352E-5	3.215791417946357E-5
14-15	2.911137494140913E-4	6.093078476108887E-5	9.816626433730985E-5	2.3695305184867892E-5	3.3850435978382705E-5
16-17	3.605071431697758E-4	6.431582835892714E-5	1.4217183110920735E-4	2.3695305184867892E-5	3.3850435978382705E-5
18-19	4.603659293060048E-4	6.770087195676541E-5	1.7771478888650922E-4	2.538782698378703E-5	3.3850435978382705E-5
20-21	5.636097590400721E-4	6.770087195676541E-5	2.1156522486489188E-4	2.7080348782706165E-5	3.3850435978382705E-5
22-23	6.88856372160088E-4	7.447095915244194E-5	2.606483570335468E-4	2.7080348782706165E-5	3.3850435978382705E-5
24-25	9.207318586120096E-4	8.293356814703762E-5	2.724960096259808E-4	2.7080348782706165E-5	3.3850435978382705E-5
26-27	0.0012355409132109687	8.970365534271417E-5	2.8095861862057647E-4	3.215791417946357E-5	3.3850435978382705E-5
28-29	0.001563890142201281	9.478122073947157E-5	3.215791417946357E-4	3.723547957622098E-5	3.723547957622097E-5
30-31	0.002051336420289992	1.049363515329864E-4	3.283492289903122E-4	3.723547957622098E-5	4.0620523174059245E-5
32-33	0.0026301788755203363	1.1339896052758206E-4	3.401968815827462E-4	4.0620523174059245E-5	4.0620523174059245E-5
34-35	0.003239486723131225	1.150914823265012E-4	4.197454061319456E-4	4.0620523174059245E-5	4.0620523174059245E-5
36-37	0.003996043967248078	1.150914823265012E-4	4.434407113168134E-4	4.0620523174059245E-5	4.0620523174059245E-5
38-39	0.005042022438980104	1.150914823265012E-4	4.620584511049239E-4	4.0620523174059245E-5	4.0620523174059245E-5
40-41	0.006460355706474339	1.2524661312001602E-4	4.857537562897918E-4	4.0620523174059245E-5	4.0620523174059245E-5
42-43	0.008107179416822659	1.2693913491893514E-4	5.263742794638511E-4	4.0620523174059245E-5	4.400556677189752E-5
44-45	0.009919870263465053	1.3032417851677343E-4	5.432994974530424E-4	4.0620523174059245E-5	4.400556677189752E-5
46-47	0.012013519728728023	1.3540174391353082E-4	5.957676732195355E-4	5.077565396757406E-5	4.908313216865492E-5
48-49	0.014652161213242955	1.438643529081265E-4	6.313106309968374E-4	5.077565396757406E-5	5.077565396757406E-5
50-51	0.01795088619933635	1.6078957089731786E-4	6.346956745946757E-4	5.077565396757406E-5	5.077565396757406E-5
52-53	0.02214326269525905	1.726372234897518E-4	6.533134143827862E-4	5.077565396757406E-5	5.24681757664932E-5
54-55	0.02687216860143911	1.7602226708759007E-4	6.75316197768735E-4	5.24681757664932E-5	6.770087195676541E-5
56-57	0.032460875581470094	1.827923542832666E-4	7.091666337471177E-4	5.416069756541233E-5	6.770087195676541E-5
58-59	0.038630117538530344	1.8956244147894314E-4	7.311694171330664E-4	5.416069756541233E-5	6.770087195676541E-5
60-61	0.04652911677408595	1.9294748507678143E-4	7.853301146984787E-4	5.416069756541233E-5	6.770087195676541E-5
62-63	0.05661823921744291	1.9802505047353882E-4	8.564160302530824E-4	5.416069756541233E-5	6.770087195676541E-5
64-65	0.06888225217241098	2.0310261587029623E-4	9.0042159702498E-4	5.5853219364331464E-5	7.954852454919936E-5
66-67	0.08336854624935985	2.064876594681345E-4	9.0042159702498E-4	6.093078476108887E-5	8.124104634811849E-5
68-69	0.10055610511738367	2.2679792105516415E-4	9.088842060195756E-4	6.770087195676541E-5	8.124104634811849E-5
70-71	0.1214333615070512	2.555707916367894E-4	9.139617714163331E-4	6.770087195676541E-5	8.293356814703762E-5
72-73	0.14763359895431943	2.640334006313851E-4	9.342720330033627E-4	7.108591555460368E-5	9.478122073947157E-5
74-75	0.1810389016995864	2.640334006313851E-4	9.410421201990392E-4	7.447095915244196E-5	9.478122073947157E-5
76-77	0.22234320368040894	2.691109660281425E-4	9.545822945903923E-4	7.447095915244196E-5	9.478122073947157E-5
78-79	0.272093189437838	2.809586186205764E-4	9.613523817860689E-4	7.447095915244196E-5	9.647374253839072E-5
80-81	0.33093201725546284	2.8603618401733385E-4	9.748925561774219E-4	7.447095915244196E-5	9.985878613622898E-5
82-83	0.40236320725704605	2.9280627121301044E-4	9.850476869709367E-4	7.785600275028022E-5	1.0155130793514811E-4
84-85	0.4915794163216715	2.978838366097678E-4	9.968953395633707E-4	7.954852454919936E-5	1.0155130793514811E-4
86-87	0.603441567055835	3.029614020065252E-4	9.985878613622899E-4	8.124104634811849E-5	1.0155130793514811E-4
88-89	0.7385217318275712	3.1480905459895913E-4	0.0010019729049601282	8.124104634811849E-5	1.0493635153298638E-4
90	0.8542157519144876	3.215791417946357E-4	0.0010019729049601282	8.124104634811849E-5	1.0493635153298638E-4
>>END_MODULE
