##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	SRR19654127_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	32665390
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.335098524768878	32.0	32.0	32.0	32.0	32.0
2	31.434714387307178	32.0	32.0	32.0	32.0	32.0
3	33.86263825412769	37.0	32.0	37.0	32.0	37.0
4	35.43422625598531	37.0	37.0	37.0	32.0	37.0
5	35.494422077924064	37.0	37.0	37.0	32.0	37.0
6	38.929110964234624	41.0	41.0	41.0	37.0	41.0
7	39.11980937622358	41.0	41.0	41.0	37.0	41.0
8	39.2816953050308	41.0	41.0	41.0	37.0	41.0
9	39.17620940695948	41.0	41.0	41.0	37.0	41.0
10-11	39.17820018374188	41.0	41.0	41.0	37.0	41.0
12-13	39.39500264653201	41.0	41.0	41.0	37.0	41.0
14-15	39.454226644776014	41.0	41.0	41.0	37.0	41.0
16-17	39.16429237489588	41.0	41.0	41.0	37.0	41.0
18-19	39.344457941570575	41.0	41.0	41.0	37.0	41.0
20-21	39.37452531563223	41.0	41.0	41.0	37.0	41.0
22-23	39.106000326339284	41.0	41.0	41.0	37.0	41.0
24-25	39.11603547363127	41.0	41.0	41.0	37.0	41.0
26-27	39.082599825074794	41.0	41.0	41.0	37.0	41.0
28-29	38.83800407709812	41.0	41.0	41.0	34.5	41.0
30-31	39.05528377282499	41.0	41.0	41.0	37.0	41.0
32-33	39.25393912333513	41.0	41.0	41.0	37.0	41.0
34-35	39.12936784162075	41.0	41.0	41.0	37.0	41.0
36-37	38.82536011356362	41.0	41.0	41.0	32.0	41.0
38-39	38.83659417505807	41.0	41.0	41.0	32.0	41.0
40-41	38.814700191854435	41.0	41.0	41.0	32.0	41.0
42-43	38.70546538706564	41.0	41.0	41.0	32.0	41.0
44-45	38.742628268023125	41.0	41.0	41.0	32.0	41.0
46-47	38.72838095611288	41.0	41.0	41.0	32.0	41.0
48-49	38.77830290714422	41.0	41.0	41.0	32.0	41.0
50-51	38.69617214121735	41.0	41.0	41.0	32.0	41.0
52-53	38.787490460086346	41.0	41.0	41.0	32.0	41.0
54-55	38.66937506333156	41.0	41.0	41.0	32.0	41.0
56-57	38.65679379612489	41.0	41.0	41.0	32.0	41.0
58-59	38.65210704663254	41.0	41.0	41.0	32.0	41.0
60-61	38.67706649147615	41.0	41.0	41.0	32.0	41.0
62-63	38.693402053365965	41.0	41.0	41.0	32.0	41.0
64-65	38.69422148334981	41.0	41.0	41.0	32.0	41.0
66-67	38.642700286143835	41.0	41.0	41.0	32.0	41.0
68-69	38.601013672269026	41.0	41.0	41.0	32.0	41.0
70-71	38.560727148826324	41.0	41.0	41.0	32.0	41.0
72-73	38.515295439607485	41.0	41.0	41.0	32.0	41.0
74-75	38.49236756395683	41.0	41.0	41.0	32.0	41.0
76-77	37.640450611488184	41.0	39.0	41.0	29.5	41.0
78-79	38.2176785888673	41.0	37.0	41.0	32.0	41.0
80-81	38.659517871973975	41.0	41.0	41.0	32.0	41.0
82-83	38.70483478078786	41.0	41.0	41.0	32.0	41.0
84-85	38.581764935303084	41.0	41.0	41.0	32.0	41.0
86-87	38.62248068980655	41.0	41.0	41.0	32.0	41.0
88-89	38.5032717350076	41.0	41.0	41.0	32.0	41.0
90-91	38.399699590300315	41.0	39.0	41.0	32.0	41.0
92-93	38.38217489520254	41.0	37.0	41.0	32.0	41.0
94-95	38.20739809014985	41.0	37.0	41.0	32.0	41.0
96-97	38.16603732574447	41.0	37.0	41.0	32.0	41.0
98-99	38.08668064272308	41.0	37.0	41.0	32.0	41.0
100-101	36.94448951933529	41.0	37.0	41.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	34.0
13	121.0
14	471.0
15	2050.0
16	8703.0
17	22892.0
18	39493.0
19	53198.0
20	65164.0
21	78192.0
22	91997.0
23	108165.0
24	125541.0
25	144771.0
26	166555.0
27	191806.0
28	222621.0
29	258116.0
30	301885.0
31	355917.0
32	423320.0
33	510328.0
34	636514.0
35	812867.0
36	1073740.0
37	1534115.0
38	2647410.0
39	6911547.0
40	1.5877856E7
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	28.373320244492394	20.558811947657617	12.188615733220324	38.87925207462966
2	27.566714856213427	24.61493494686725	31.669557621062882	16.14879257585644
3	20.715788204010245	27.881100253697937	28.93035414542185	22.472757396869966
4	24.528660180137727	30.733368479659738	23.304411443579383	21.433559896623144
5	28.256481438815044	32.20379865566529	21.810331141968046	17.72938876355162
6	22.57763215892005	37.2749685457267	21.782074312354695	18.36532498299856
7	22.347473729324182	22.08788281523885	35.41488455334562	20.149758902091346
8	22.668171072261895	24.223319178682228	28.10372977204814	25.004779977007736
9	23.78315279468802	22.898504758560495	29.909460759747112	23.40888168700437
10-11	25.039761426085782	30.15092725379997	23.413998651848893	21.395312668265355
12-13	25.691423756322312	25.085739230707787	25.86736127309352	23.355475739876375
14-15	24.952930040583656	26.851982637529066	25.75282658181325	22.44226074007403
16-17	25.97487840022316	26.283027498613514	25.124889194533807	22.617204906629517
18-19	25.499064761629032	26.879221064558816	25.222713855636375	22.399000318175773
20-21	25.822590991670264	26.874008043327585	25.12586508663671	22.177535878365433
22-23	25.602699177505855	26.63108666448731	25.34322152049247	22.422992637514362
24-25	24.9856348749949	26.763086309314453	25.80038909645105	22.45088971923959
26-27	25.31547596905348	26.829117674568625	25.687593179323287	22.167813177054608
28-29	25.466448253260925	26.4651230436833	25.46558948696153	22.602839216094242
30-31	25.159005223836274	26.607447166140773	25.700917768819988	22.53262984120297
32-33	25.3552685959	26.823202435405875	25.481312449903186	22.340216518790935
34-35	25.51667075861171	26.591021785286816	25.355115594373224	22.53719186172825
36-37	25.19202383220902	26.668716325144004	25.670879427176587	22.46838041547039
38-39	25.44987858818965	26.857423428017025	25.44409051270286	22.24860747109047
40-41	25.642301548651155	26.482797002760393	25.342549727599867	22.532351720988586
42-43	25.109755161868158	26.67591746252618	25.780006413700065	22.4343209619056
44-45	25.36614630954667	26.706986527472743	25.60574662340288	22.321120539577702
46-47	25.560632418291306	26.513806147183676	25.481037102563963	22.444524331961052
48-49	25.15721809897713	26.56886904829881	25.783327354438295	22.490585498285768
50-51	25.491354047402137	26.652136116148228	25.450110385197615	22.406399451252017
52-53	25.683086140989687	26.478637865161925	25.397530553882962	22.44074543996543
54-55	25.171143254853163	26.59812108671355	25.76764441453538	22.46309124389791
56-57	25.33125456235863	26.774830605261528	25.59202506600628	22.30188976637356
58-59	25.583139242899744	26.392815974102934	25.499438700140477	22.52460608285685
60-61	25.256228089969955	26.554285197692458	25.69244259273257	22.497044119605018
62-63	25.421800747040308	26.618457717785617	25.62857236283982	22.331169172334256
64-65	25.53552509285891	26.42172385704844	25.61331642341243	22.429434626680216
66-67	25.18934352848092	26.638466280068613	25.837506661128966	22.33468353032151
68-69	25.485584413999828	26.797293716421354	25.551638489978135	22.16548337960068
70-71	25.767489013828165	26.51779974450863	25.3533870731016	22.361324168561605
72-73	25.251716700588624	26.64831567963421	25.814927149550137	22.285040470227024
74-75	25.329855845518683	26.806560400262896	25.64440747469132	22.219176279527094
76-77	25.63932271706847	26.47239436508468	25.54271728461293	22.34556563323392
78-79	25.19550345797291	26.651214482804924	25.908366404442067	22.244915654780094
80-81	25.54823059095571	26.72320684678323	25.608306420158822	22.120256142102235
82-83	25.72319897871101	26.403596517984536	25.580060115046866	22.293144388257588
84-85	25.389407067145093	26.62076804725406	25.88281061731445	22.10701426828639
86-87	25.526519905052165	26.79371768986384	25.638714510894218	22.041047894189784
88-89	25.64665516392086	26.587107275379307	25.53261917234146	22.23361838835837
90-91	25.292970276243626	26.74374393305452	25.845041171613225	22.118244619088625
92-93	25.623469892905476	26.86097809100807	25.579591042315514	21.93596097377094
94-95	25.91946043490466	26.561796918248355	25.481146919039794	22.03759572780719
96-97	25.473640357354622	26.67352313970869	25.819470375113614	22.033366127823076
98-99	25.713718962679057	26.93002682509096	25.533009263790756	21.82324494843923
100-101	26.014129218569295	26.57665360090738	25.499501530251003	21.909715650272325
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	53.0
1	36.0
2	27.5
3	32.5
4	45.5
5	70.0
6	81.5
7	90.5
8	113.0
9	151.5
10	198.0
11	237.0
12	299.5
13	388.0
14	457.5
15	552.5
16	689.0
17	882.0
18	1111.5
19	1396.0
20	1839.0
21	2431.0
22	3257.0
23	4271.5
24	5651.5
25	7689.0
26	10285.0
27	13767.5
28	18958.5
29	25930.5
30	34752.5
31	47047.0
32	65232.0
33	90369.5
34	126761.0
35	183089.0
36	262287.0
37	369182.5
38	523601.5
39	731676.0
40	970744.5
41	1229108.5
42	1512117.0
43	1772489.0
44	1975746.5
45	2101564.0
46	2141438.0
47	2129190.5
48	2057757.0
49	1915355.5
50	1726248.0
51	1554487.0
52	1396209.5
53	1227955.5
54	1069311.0
55	918035.5
56	758340.0
57	607049.0
58	490735.0
59	405911.5
60	343497.5
61	296772.0
62	257228.0
63	217091.0
64	188957.5
65	166552.5
66	145970.5
67	125239.0
68	98910.0
69	77388.5
70	66572.0
71	56887.5
72	43661.5
73	31630.5
74	20608.0
75	12597.0
76	7425.5
77	3936.5
78	2246.0
79	1417.0
80	934.0
81	668.5
82	510.5
83	396.5
84	296.0
85	246.0
86	220.0
87	199.5
88	184.5
89	149.0
90	122.5
91	103.0
92	93.5
93	93.5
94	81.5
95	68.5
96	65.5
97	69.5
98	85.0
99	142.5
100	704.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.007506415811964896
2	0.0061624857379630246
3	0.0017143527139887203
4	1.5612855073825844E-4
5	0.014158716611067554
6	0.0015674081956468296
7	0.005170610239155265
8	0.009487105465448292
9	0.003367478545334986
10-11	0.006543623082412303
12-13	0.004656304424958649
14-15	0.009500881514042844
16-17	1.040857004921723E-4
18-19	0.012851522666651157
20-21	0.006254326061926705
22-23	0.00871717741621943
24-25	0.011701987945039077
26-27	0.009903448267416981
28-29	0.00690180034587066
30-31	0.010928998551678091
32-33	0.0053833736563377935
34-35	0.00497315354263335
36-37	0.009300363473388806
38-39	0.01044224483467058
40-41	0.00878452698712613
42-43	0.012416811799889729
44-45	0.008147767407644604
46-47	0.004308841865962721
48-49	0.010038147409230381
50-51	0.010414692737481475
52-53	0.0073395725567642085
54-55	0.010485103652520297
56-57	0.012563756318231622
58-59	0.005883903421939857
60-61	0.002574590415115203
62-63	0.012961731055407573
64-65	0.005279287955845621
66-67	0.00614717901730241
68-69	0.01539396896837907
70-71	0.006294123535644301
72-73	0.005919108879459269
74-75	0.013641349452738816
76-77	0.006468620151175296
78-79	0.012866829387311771
80-81	1.9898736858797644E-5
82-83	0.007203342742884747
84-85	0.01687259818419434
86-87	0.002198045086864109
88-89	0.01020499066443107
90-91	0.0036659595982169507
92-93	0.011844340447182784
94-95	0.010350404510706897
96-97	0.004181796084479628
98-99	0.009364651700163384
100-101	0.010494287684916665
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3.266539E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.986831287050105
#Duplication Level	Percentage of deduplicated	Percentage of total
1	61.678576650301785	19.72902225338591
2	15.563111059564614	9.956292155278335
3	7.125489723668325	6.837655128857639
4	3.8817263198718526	4.966564995849714
5	2.4893068293537843	3.9812518786120563
6	1.7285952215354217	3.3175370228912717
7	1.2552322694034472	2.810563197922836
8	0.9141700132085715	2.3393121584146352
9	0.718119826060003	2.0673339948060376
>10	4.189496451073261	24.561555886040694
>50	0.2916217028333318	6.412510746034035
>100	0.15125413802353463	8.949960397996996
>500	0.00965513316297289	2.1080803092191926
>1k	0.0036145819985808347	1.8871829519014167
>5k	2.005329364985985E-5	0.03799256942670405
>10k+	1.0026646824929925E-5	0.037184353362587096
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.4490753056981716E-5	3.0613441321227146E-6	3.0613441321227146E-6	3.0613441321227146E-6	0.0
2	4.592016198184072E-5	2.4490753056981716E-5	3.061344132122715E-5	6.122688264245429E-6	0.0
3	6.734957090669972E-5	3.061344132122715E-5	3.061344132122715E-5	6.122688264245429E-6	9.184032396368144E-6
4	9.490166809580415E-5	3.061344132122715E-5	3.367478545334986E-5	9.184032396368144E-6	9.184032396368144E-6
5	1.07147044624295E-4	3.061344132122715E-5	3.979747371759529E-5	1.2245376528490858E-5	9.184032396368144E-6
6	1.3469914181339944E-4	3.061344132122715E-5	6.12268826424543E-5	1.2245376528490858E-5	9.184032396368144E-6
7	1.5612855073825844E-4	3.061344132122715E-5	7.653360330306787E-5	1.2245376528490858E-5	9.184032396368144E-6
8	1.7755795966311745E-4	3.367478545334986E-5	1.0102435636004958E-4	1.2245376528490858E-5	9.184032396368144E-6
9	1.867419920594856E-4	3.979747371759529E-5	1.1326973288854044E-4	1.2245376528490858E-5	9.184032396368144E-6
10-11	2.2653946577708088E-4	4.2858817849718005E-5	1.28576453549154E-4	1.2245376528490858E-5	1.0714704462429501E-5
12-13	2.7858231602316704E-4	4.438948991577936E-5	1.4847519040795166E-4	1.2245376528490858E-5	1.8368064792736288E-5
14-15	3.444012148638054E-4	5.051217818002479E-5	1.760272875970561E-4	1.2245376528490858E-5	2.4490753056981716E-5
16-17	3.9185204891170746E-4	5.663486644427022E-5	2.3113148197526496E-4	1.2245376528490858E-5	2.755209718910443E-5
18-19	4.699163242808367E-4	6.4288226774577E-5	2.831743322213511E-4	1.5306720660613574E-5	2.755209718910443E-5
20-21	6.015541219621134E-4	8.112561950125193E-5	3.183797897407623E-4	1.5306720660613574E-5	2.755209718910443E-5
22-23	7.530906565021878E-4	8.571763569943601E-5	4.086894416383824E-4	1.5306720660613574E-5	2.755209718910443E-5
24-25	0.0010316729725253547	9.949368429398823E-5	4.3624153882748683E-4	2.1429408924859002E-5	2.908276925516579E-5
26-27	0.0014388317420976759	1.1020838875641772E-4	4.408335550256709E-4	2.4490753056981716E-5	3.061344132122715E-5
28-29	0.0017970090055560335	1.2704578148309265E-4	4.806310287432662E-4	2.4490753056981716E-5	3.061344132122715E-5
30-31	0.002260802641572625	1.5306720660613574E-4	4.8216170080932756E-4	2.4490753056981716E-5	3.673612958547257E-5
32-33	0.0028302126501474497	1.7755795966311745E-4	4.8522304494145026E-4	2.4490753056981716E-5	3.979747371759529E-5
34-35	0.0034685029016950355	1.836806479273629E-4	5.785940409711931E-4	2.4490753056981716E-5	3.979747371759529E-5
36-37	0.004337924635217887	1.9898736858797646E-4	6.061461381602976E-4	2.4490753056981716E-5	3.979747371759529E-5
38-39	0.005371128279809303	2.250087937110195E-4	6.398209236136473E-4	2.4490753056981716E-5	4.2858817849718005E-5
40-41	0.006753325155462708	2.3113148197526496E-4	6.7655705319912E-4	2.755209718910443E-5	4.2858817849718005E-5
42-43	0.008620745076057564	2.3878484230557172E-4	7.484986403040037E-4	2.755209718910443E-5	4.2858817849718005E-5
44-45	0.010512655749709401	2.479688747019399E-4	7.638053609646174E-4	2.755209718910443E-5	4.2858817849718005E-5
46-47	0.012690802099714713	2.510302188340626E-4	8.250322436070716E-4	2.755209718910443E-5	4.2858817849718005E-5
48-49	0.015507238701267611	2.5562223503224665E-4	8.5717635699436E-4	2.755209718910443E-5	4.2858817849718005E-5
50-51	0.0190568672224639	2.7245962775892163E-4	8.724830776549737E-4	2.755209718910443E-5	4.2858817849718005E-5
52-53	0.023446834707927872	2.9082769255165785E-4	8.954431586458941E-4	2.908276925516579E-5	4.438948991577936E-5
54-55	0.028525604623119455	2.9541970874984196E-4	9.33709960297428E-4	3.061344132122715E-5	4.592016198184072E-5
56-57	0.03393959172077848	3.061344132122715E-4	9.888141546756369E-4	3.21441133872885E-5	4.592016198184072E-5
58-59	0.040628628649466605	3.1378777354257824E-4	0.001011774235666557	3.367478545334986E-5	4.592016198184072E-5
60-61	0.04894170864024584	3.153184456086396E-4	0.0010408570049217228	3.520545751941122E-5	4.898150611396343E-5
62-63	0.05927221441409394	3.21441133872885E-4	0.0011326973288854044	3.6736129585472576E-5	5.2042850246086144E-5
64-65	0.07207016355843296	3.2756382213713046E-4	0.001224537652849086	3.6736129585472576E-5	5.816553851033158E-5
66-67	0.0876508745188715	3.352171824674373E-4	0.0012352523573115155	3.6736129585472576E-5	6.4288226774577E-5
68-69	0.10641232203258555	3.474625589959281E-4	0.0012459670617739449	3.6736129585472576E-5	6.4288226774577E-5
70-71	0.12902340979244392	3.5970793552441896E-4	0.0012536204221042517	3.6736129585472576E-5	6.581889884063835E-5
72-73	0.15789647697455933	3.734839841189712E-4	0.001281172519293356	3.6736129585472576E-5	7.500293123700652E-5
74-75	0.19398513227608793	3.8726003271352335E-4	0.0012827031913594175	3.8266801651533935E-5	7.653360330306787E-5
76-77	0.2382276776735254	3.9185204891170746E-4	0.0012964792399539696	3.979747371759529E-5	7.959494743519057E-5
78-79	0.2915501697668389	3.979747371759529E-4	0.0013148473047467058	4.2858817849718005E-5	7.959494743519057E-5
80-81	0.3551159193262349	4.0103608130807563E-4	0.0013255620092091354	4.438948991577936E-5	7.959494743519057E-5
82-83	0.4327378304682724	4.0103608130807563E-4	0.0013485220902000558	4.592016198184072E-5	7.959494743519057E-5
84-85	0.5302982759428251	4.0409742544019833E-4	0.0013592367946624853	4.7450834047902074E-5	7.959494743519057E-5
86-87	0.6526158114138543	4.0715876957232104E-4	0.0013714821711909761	4.898150611396343E-5	7.959494743519057E-5
88-89	0.8005231224852971	4.1022011370444374E-4	0.0013714821711909761	4.898150611396343E-5	7.959494743519057E-5
90	0.9263841637892583	4.163428019686892E-4	0.0013714821711909761	5.510419437820886E-5	7.959494743519057E-5
>>END_MODULE
