##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	SRR19654131_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	28494240
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.623473375671715	32.0	32.0	32.0	32.0	32.0
2	31.681763577480922	32.0	32.0	32.0	32.0	32.0
3	35.712034958644274	37.0	37.0	37.0	32.0	37.0
4	36.350685612250054	37.0	37.0	37.0	37.0	37.0
5	36.42949259218705	37.0	37.0	37.0	37.0	37.0
6	40.08153739141665	41.0	41.0	41.0	37.0	41.0
7	40.095397245197624	41.0	41.0	41.0	37.0	41.0
8	40.2320779217133	41.0	41.0	41.0	37.0	41.0
9	40.20985058734678	41.0	41.0	41.0	41.0	41.0
10-11	40.27999816805081	41.0	41.0	41.0	41.0	41.0
12-13	40.24104813815002	41.0	41.0	41.0	41.0	41.0
14-15	40.27305236075782	41.0	41.0	41.0	41.0	41.0
16-17	40.2739911996249	41.0	41.0	41.0	41.0	41.0
18-19	40.26744898267158	41.0	41.0	41.0	41.0	41.0
20-21	40.19875106337281	41.0	41.0	41.0	41.0	41.0
22-23	40.156801672899505	41.0	41.0	41.0	39.0	41.0
24-25	40.194251820718854	41.0	41.0	41.0	41.0	41.0
26-27	40.168618096148556	41.0	41.0	41.0	41.0	41.0
28-29	40.0862704181617	41.0	41.0	41.0	39.0	41.0
30-31	40.10046639250599	41.0	41.0	41.0	37.0	41.0
32-33	40.11174833931349	41.0	41.0	41.0	39.0	41.0
34-35	40.10520931598808	41.0	41.0	41.0	37.0	41.0
36-37	40.08617732917249	41.0	41.0	41.0	37.0	41.0
38-39	40.06551180168343	41.0	41.0	41.0	37.0	41.0
40-41	40.05602734096435	41.0	41.0	41.0	37.0	41.0
42-43	39.99866560750523	41.0	41.0	41.0	37.0	41.0
44-45	40.01748393710448	41.0	41.0	41.0	37.0	41.0
46-47	40.00250412013094	41.0	41.0	41.0	37.0	41.0
48-49	39.98063501605939	41.0	41.0	41.0	37.0	41.0
50-51	39.90946249136668	41.0	41.0	41.0	37.0	41.0
52-53	39.932070621992374	41.0	41.0	41.0	37.0	41.0
54-55	39.87954755066287	41.0	41.0	41.0	37.0	41.0
56-57	39.87861762587808	41.0	41.0	41.0	37.0	41.0
58-59	39.873599576616186	41.0	41.0	41.0	37.0	41.0
60-61	39.79654924995367	41.0	41.0	41.0	37.0	41.0
62-63	39.75891446832763	41.0	41.0	41.0	37.0	41.0
64-65	39.752239908837716	41.0	41.0	41.0	37.0	41.0
66-67	39.71778103223669	41.0	41.0	41.0	37.0	41.0
68-69	39.63778739141665	41.0	41.0	41.0	37.0	41.0
70-71	39.655202033814554	41.0	41.0	41.0	37.0	41.0
72-73	39.565385056769365	41.0	41.0	41.0	37.0	41.0
74-75	39.607361382510994	41.0	41.0	41.0	37.0	41.0
76-77	39.06486656601474	41.0	39.0	41.0	34.5	41.0
78-79	39.38216514986888	41.0	41.0	41.0	37.0	41.0
80-81	39.59295833122765	41.0	41.0	41.0	37.0	41.0
82-83	39.734846814654475	41.0	41.0	41.0	37.0	41.0
84-85	39.692945626905654	41.0	41.0	41.0	37.0	41.0
86-87	39.71103907316005	41.0	41.0	41.0	37.0	41.0
88-89	39.64655926952254	41.0	41.0	41.0	37.0	41.0
90-91	39.66655485459518	41.0	41.0	41.0	37.0	41.0
92-93	39.61795399701835	41.0	41.0	41.0	37.0	41.0
94-95	39.4750436403989	41.0	41.0	41.0	37.0	41.0
96-97	39.438788365648634	41.0	41.0	41.0	37.0	41.0
98-99	39.49018075232047	41.0	41.0	41.0	37.0	41.0
100-101	38.95495901978786	41.0	39.0	41.0	34.5	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	4.0
17	10.0
18	43.0
19	148.0
20	507.0
21	1439.0
22	3664.0
23	7720.0
24	14455.0
25	24785.0
26	40705.0
27	63150.0
28	91868.0
29	126914.0
30	167073.0
31	211821.0
32	260422.0
33	316705.0
34	380858.0
35	464284.0
36	588826.0
37	798683.0
38	1258764.0
39	2822256.0
40	2.0849135E7
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.085819779831525	11.212029689593193	6.8399122482894965	51.86223828228579
2	21.369424136246483	13.069813407902789	35.15536824284487	30.405394213005856
3	19.743446394780136	14.40971578817333	21.00639287098024	44.84044494606629
4	23.085122010678273	21.743284485301977	20.400344768337053	34.7712487356827
5	24.37155368944741	27.04809463245905	24.201656194374724	24.378695483718815
6	20.247025363722635	31.14728450381551	25.491990661972387	23.113699470489475
7	15.635984676201225	25.781842224954936	39.37801464436321	19.204158454480623
8	17.784313601626152	24.534270785955336	32.9205130580777	24.76090255434081
9	18.05807713906449	22.528974993515178	35.15586337055932	24.257084496861015
10-11	20.43616022515897	31.309312976681298	25.536910171887662	22.71761662627207
12-13	21.107864788010712	25.337060463206434	27.74339131968298	25.811683429099862
14-15	20.681671102650924	26.073209883822134	27.53859727439651	25.706521739130434
16-17	21.34542379770103	26.36801002371532	26.775932379228063	25.51063379935559
18-19	21.04939167931324	26.542972000727517	26.730313487234124	25.677322832725125
20-21	21.36014936399226	26.520448934254926	26.9523639062755	25.167037795477317
22-23	21.264866162424408	26.755107348011386	26.935898272773724	25.04412821679048
24-25	21.147528783844187	26.452936215405927	26.786865113560935	25.612669887188954
26-27	21.042607207632138	26.550271212708253	27.034930568423654	25.372191011235955
28-29	21.139505841474413	26.652216579275713	26.84631940225259	25.36195817699729
30-31	21.137277218132507	26.23767294723425	26.97252497346832	25.65252486116492
32-33	21.276646819859184	26.258401099121407	26.925501955829308	25.5394501251901
34-35	21.36048522225312	26.4195349514135	26.659018059420177	25.560961766913202
36-37	21.20499826973198	26.264333188081245	26.61044118877704	25.92022735340973
38-39	21.366368333288733	26.420433781791235	26.90777782798502	25.305420056935013
40-41	21.498878404347604	26.57216939319566	26.678401441751742	25.250550760704993
42-43	21.500373408801217	26.18581860755016	26.69727109759727	25.616536886051357
44-45	21.473992235926804	26.179645083874952	26.920337301611674	25.42602537858657
46-47	21.44819573681362	26.348924587406973	26.734575271160637	25.46830440461877
48-49	21.408879258436023	26.08492581929892	26.736502755263132	25.769692167001928
50-51	21.46148109770009	26.154473163357174	26.86612247931871	25.517923259624027
52-53	21.823040375879476	26.331721779559658	26.50618861917356	25.339049225387306
54-55	21.65279187090416	26.136042077359956	26.481646500018442	25.729519551717445
56-57	21.534599828319156	26.049131520901753	26.895405185165806	25.520863465613285
58-59	21.51564355871343	26.195439970789668	26.829839571997333	25.45907689849957
60-61	21.406888583501395	25.959191089223786	26.807659715385064	25.82626061188975
62-63	21.647434974957058	25.87513513516359	27.011113664656122	25.466316225223228
64-65	21.700580475365555	26.057893045882413	26.73268072079059	25.50884575796144
66-67	21.60133527226728	25.93846081117436	26.70198492721075	25.758218989347615
68-69	21.6301939513178	25.963561844190618	26.89726256157366	25.50898164291792
70-71	21.775382162477026	26.156406082871175	26.721528814517015	25.34668294013478
72-73	21.76852946524334	26.007742384790827	26.582005872591775	25.64172227737406
74-75	21.741171910682006	26.01011171592719	26.82554301664379	25.42317335674702
76-77	21.737468695427566	26.084784152867385	26.680873046622754	25.49687410508229
78-79	21.586780071447322	25.879482002798465	26.756202445786524	25.777535479967696
80-81	21.69069852035858	25.89058004403237	26.877398412060632	25.54132302354842
82-83	21.797704418830204	26.12304736512085	26.560251284418783	25.518996931630166
84-85	21.8730212536691	25.82259564000188	26.505278203980353	25.799104902348667
86-87	21.86529047344478	25.81996646841701	26.753692033104226	25.561051025033986
88-89	21.997257862281938	26.0274984104217	26.708709151873666	25.266534575422696
90-91	21.924861370750076	25.802767257491197	26.619148085961402	25.65322328579732
92-93	21.95529053654943	25.82121773673717	26.758967668240434	25.464524058472964
94-95	22.062353221644074	25.93075220577323	26.519657471422843	25.48723710115985
96-97	21.91918366119324	25.990659737687853	26.33939337340464	25.75076322771426
98-99	22.008656582680082	26.077126206308844	26.646216623943282	25.26800058706779
100-101	22.011377792903613	26.175425986290435	26.50690855743744	25.306287663368515
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	51.0
1	34.5
2	22.0
3	27.0
4	39.5
5	50.0
6	69.0
7	90.5
8	91.5
9	111.5
10	146.5
11	187.5
12	214.0
13	227.0
14	281.5
15	356.5
16	436.0
17	539.5
18	682.5
19	881.0
20	1211.5
21	1659.5
22	2309.0
23	3309.5
24	5125.5
25	7710.5
26	10952.5
27	16039.0
28	23664.5
29	34359.5
30	48748.5
31	67856.0
32	95403.5
33	131168.0
34	176024.5
35	236463.0
36	308449.5
37	388810.5
38	494988.0
39	638988.0
40	808492.0
41	1003426.5
42	1213416.0
43	1418825.5
44	1606131.5
45	1746189.5
46	1830561.0
47	1851582.0
48	1802176.0
49	1697448.0
50	1565959.5
51	1413152.5
52	1261594.5
53	1128982.0
54	974850.5
55	810006.5
56	672486.0
57	563975.5
58	461456.5
59	370864.5
60	301286.5
61	249467.0
62	209385.0
63	170054.0
64	145177.0
65	123899.0
66	101555.0
67	82443.5
68	56626.5
69	38820.5
70	32619.0
71	24237.0
72	17174.5
73	15104.0
74	12300.5
75	8083.5
76	4707.0
77	1602.0
78	238.5
79	62.5
80	26.0
81	17.0
82	9.0
83	4.5
84	3.0
85	1.0
86	1.0
87	1.0
88	0.5
89	0.0
90	0.0
91	0.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	1.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03432623575852523
2	0.0
3	0.0
4	0.019460073334119456
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0027759996406291234
10-11	0.003332252413119283
12-13	0.001631908764718764
14-15	0.0
16-17	7.527837204993009E-4
18-19	6.141592125285671E-5
20-21	3.15853309300406E-4
22-23	0.0
24-25	1.7547406072244776E-6
26-27	0.0
28-29	2.2460679772473314E-4
30-31	0.0
32-33	0.002009177995272027
34-35	5.738001785624042E-4
36-37	3.4919338083767105E-4
38-39	0.004307888190736092
40-41	4.9132737002285375E-5
42-43	0.0
44-45	0.0024250515191842283
46-47	2.1056887286693732E-4
48-49	4.825536669867313E-4
50-51	3.1585330930040597E-5
52-53	0.0
54-55	8.668418599688919E-4
56-57	3.1058908747873254E-4
58-59	7.440100174631785E-4
60-61	7.720858671787702E-5
62-63	2.930416814064878E-4
64-65	0.002142538281421087
66-67	0.0020197064389153737
68-69	0.008240261891526149
70-71	0.00864911645300945
72-73	6.211781749574651E-4
74-75	0.001270432199630522
76-77	0.0
78-79	0.0013318481208833785
80-81	0.008675437562117819
82-83	0.005566037206116043
84-85	1.0703917704069313E-4
86-87	0.01014941967218638
88-89	1.6670035768632537E-4
90-91	0.0021548214656716587
92-93	6.422350622441588E-4
94-95	0.014051962782653617
96-97	0.0010054663679396256
98-99	3.0181538444261016E-4
100-101	0.015287300170139649
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2.849424E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.5145629595427
#Duplication Level	Percentage of deduplicated	Percentage of total
1	62.305212069174864	20.258267405308462
2	16.291894476576942	10.594476573777742
3	6.89267210896521	6.723366637392999
4	3.6604535460896677	4.760721891392554
5	2.313721796627772	3.761482651365998
6	1.5477015962559693	3.019370459642968
7	1.1150233767627344	2.5378148449579685
8	0.8545036245490011	2.2227049519644755
9	0.6760153749963387	1.9782310024743595
>10	3.8921675958188002	23.29073044497295
>50	0.2801113195814121	6.270668894181251
>100	0.15459933156398667	9.215736803699233
>500	0.010749032687390485	2.4017418176713567
>1k	0.005094837390280144	2.81537787058
>5k	7.991295971788527E-5	0.1493077506175287
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.7547406072244775E-5	0.0	0.0	0.0	0.0
2	3.509481214448955E-5	0.0	0.0	0.0	0.0
3	5.6151699431183284E-5	0.0	0.0	0.0	7.0189624288979105E-6
4	1.0528443643346866E-4	0.0	0.0	0.0	7.0189624288979105E-6
5	1.1581288007681552E-4	0.0	0.0	0.0	7.0189624288979105E-6
6	1.4388872979240717E-4	0.0	7.0189624288979105E-6	0.0	7.0189624288979105E-6
7	1.719645795079988E-4	1.0528443643346867E-5	7.0189624288979105E-6	0.0	7.0189624288979105E-6
8	2.1056887286693732E-4	1.0528443643346867E-5	7.0189624288979105E-6	0.0	7.0189624288979105E-6
9	2.3513524136808001E-4	1.0528443643346867E-5	7.0189624288979105E-6	0.0	7.0189624288979105E-6
10-11	2.7900375654869196E-4	1.0528443643346867E-5	7.0189624288979105E-6	0.0	7.0189624288979105E-6
12-13	3.5270286205212E-4	4.73779963950609E-5	7.0189624288979105E-6	0.0	7.0189624288979105E-6
14-15	4.29911448769997E-4	5.264221821673433E-5	7.0189624288979105E-6	0.0	1.0528443643346867E-5
16-17	5.334411445962412E-4	5.6151699431183284E-5	7.0189624288979105E-6	0.0	1.0528443643346867E-5
18-19	6.124044719213427E-4	5.966118064563224E-5	7.0189624288979105E-6	0.0	1.0528443643346867E-5
20-21	7.0540572410424E-4	5.966118064563224E-5	7.0189624288979105E-6	0.0	1.4037924857795821E-5
22-23	7.79104829607668E-4	6.492540246730568E-5	7.0189624288979105E-6	0.0	1.4037924857795821E-5
24-25	8.721060817905653E-4	7.369910550342806E-5	7.0189624288979105E-6	0.0	1.4037924857795821E-5
26-27	0.001017749552190197	1.4388872979240717E-4	7.0189624288979105E-6	0.0	1.5792665465020298E-5
28-29	0.0011897141316981959	1.4388872979240717E-4	1.4037924857795821E-5	0.0	1.9302146679469256E-5
30-31	0.0013897545609217864	1.4388872979240717E-4	1.5792665465020298E-5	0.0	2.1056887286693733E-5
32-33	0.0016670035768632538	1.4388872979240717E-4	2.4566368501142688E-5	0.0	2.281162789391821E-5
34-35	0.002061820213488761	1.5441717343575402E-4	3.1585330930040597E-5	0.0	2.4566368501142688E-5
36-37	0.0026163182453716963	1.5441717343575402E-4	3.1585330930040597E-5	0.0	2.4566368501142688E-5
38-39	0.003355064041013201	1.5441717343575402E-4	3.1585330930040597E-5	0.0	2.4566368501142688E-5
40-41	0.00435000196530948	1.5441717343575402E-4	3.1585330930040597E-5	0.0	2.4566368501142688E-5
42-43	0.005458998029075349	1.8249302315134568E-4	3.3340071537265074E-5	0.0	2.6321109108367165E-5
44-45	0.006785581928137055	2.1232361347416182E-4	3.509481214448955E-5	0.0	2.8075849715591642E-5
46-47	0.00829641359095733	2.4741842561865133E-4	3.509481214448955E-5	0.0	2.8075849715591642E-5
48-49	0.010305591586229356	2.5092790683310033E-4	3.509481214448955E-5	0.0	2.8075849715591642E-5
50-51	0.013009646861962277	2.5268264744032477E-4	3.509481214448955E-5	0.0	2.8075849715591642E-5
52-53	0.016322597128402092	2.667205722981206E-4	3.509481214448955E-5	1.7547406072244776E-6	2.8075849715591642E-5
54-55	0.0202374234231199	2.7023005351256953E-4	3.684955275171403E-5	3.5094812144489553E-6	3.1585330930040597E-5
56-57	0.02447687673017424	3.211175311220794E-4	5.088747760950985E-5	3.5094812144489553E-6	3.860429335893851E-5
58-59	0.029955176905929054	3.2813649355097735E-4	5.6151699431183284E-5	3.5094812144489553E-6	4.2113774573387467E-5
60-61	0.037093461696118234	3.3691019658709973E-4	5.966118064563224E-5	3.5094812144489553E-6	4.2113774573387467E-5
62-63	0.0462093391506494	3.509481214448955E-4	5.966118064563224E-5	3.5094812144489553E-6	4.9132737002285375E-5
64-65	0.057529170807854496	3.544576026593445E-4	5.966118064563224E-5	3.5094812144489553E-6	5.264221821673433E-5
66-67	0.07111086310777195	3.544576026593445E-4	5.966118064563224E-5	3.5094812144489553E-6	5.264221821673433E-5
68-69	0.08760542481568204	3.5796708387379343E-4	6.141592125285671E-5	3.5094812144489553E-6	5.264221821673433E-5
70-71	0.10791479260369816	3.5796708387379343E-4	6.317066186008119E-5	3.5094812144489553E-6	5.6151699431183284E-5
72-73	0.13370947952989798	3.5796708387379343E-4	7.01896242889791E-5	3.5094812144489553E-6	5.6151699431183284E-5
74-75	0.16711447646963035	3.684955275171403E-4	7.369910550342806E-5	3.5094812144489553E-6	5.6151699431183284E-5
76-77	0.20822453941568542	4.4394937362779284E-4	7.369910550342806E-5	5.264221821673433E-6	5.6151699431183284E-5
78-79	0.25825921309008415	4.8430840759395585E-4	7.369910550342806E-5	7.0189624288979105E-6	5.790644003840776E-5
80-81	0.32133862843858974	4.913273700228538E-4	7.369910550342806E-5	7.0189624288979105E-6	5.966118064563224E-5
82-83	0.39833313680238536	4.913273700228538E-4	7.369910550342806E-5	7.0189624288979105E-6	5.966118064563224E-5
84-85	0.49297858093425195	5.211579603456699E-4	7.720858671787701E-5	7.0189624288979105E-6	5.966118064563224E-5
86-87	0.6129115919568305	5.650264755262818E-4	8.422754914677493E-5	7.0189624288979105E-6	6.141592125285671E-5
88-89	0.757779467008069	6.264423967791385E-4	8.422754914677493E-5	7.0189624288979105E-6	6.317066186008119E-5
90	0.881890515416449	6.632919495308526E-4	8.773703036122389E-5	7.0189624288979105E-6	6.317066186008119E-5
>>END_MODULE
