##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	SRR19654123_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	32139809
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.34888884996174	32.0	32.0	32.0	32.0	32.0
2	31.33924856242923	32.0	32.0	32.0	32.0	32.0
3	34.882226680314126	37.0	32.0	37.0	32.0	37.0
4	35.67808159656456	37.0	37.0	37.0	32.0	37.0
5	36.03092663058452	37.0	37.0	37.0	37.0	37.0
6	38.95375012962896	41.0	41.0	41.0	37.0	41.0
7	39.184084696956354	41.0	41.0	41.0	37.0	41.0
8	39.432708452001066	41.0	41.0	41.0	37.0	41.0
9	39.71096415040923	41.0	41.0	41.0	37.0	41.0
10-11	39.641243449828835	41.0	41.0	41.0	37.0	41.0
12-13	39.54580920191529	41.0	41.0	41.0	37.0	41.0
14-15	39.44345518668142	41.0	41.0	41.0	37.0	41.0
16-17	39.531788287851995	41.0	41.0	41.0	37.0	41.0
18-19	39.56160822237618	41.0	41.0	41.0	37.0	41.0
20-21	39.45787705210071	41.0	41.0	41.0	37.0	41.0
22-23	39.33585148250259	41.0	41.0	41.0	37.0	41.0
24-25	39.35559254568065	41.0	41.0	41.0	37.0	41.0
26-27	39.19214152766122	41.0	41.0	41.0	37.0	41.0
28-29	39.37411238193731	41.0	41.0	41.0	37.0	41.0
30-31	39.3637068596145	41.0	41.0	41.0	37.0	41.0
32-33	39.37369976280817	41.0	41.0	41.0	37.0	41.0
34-35	39.183864424956596	41.0	41.0	41.0	37.0	41.0
36-37	39.12391168846087	41.0	41.0	41.0	37.0	41.0
38-39	39.09287410513236	41.0	41.0	41.0	37.0	41.0
40-41	39.04806224890758	41.0	41.0	41.0	37.0	41.0
42-43	39.04308420127823	41.0	41.0	41.0	37.0	41.0
44-45	39.045834979915405	41.0	41.0	41.0	37.0	41.0
46-47	39.006726253413646	41.0	41.0	41.0	37.0	41.0
48-49	38.962555440201896	41.0	41.0	41.0	37.0	41.0
50-51	39.02053580965587	41.0	41.0	41.0	37.0	41.0
52-53	39.03004078213408	41.0	41.0	41.0	37.0	41.0
54-55	39.02295914390779	41.0	41.0	41.0	37.0	41.0
56-57	38.989298318480984	41.0	41.0	41.0	37.0	41.0
58-59	38.899165906679784	41.0	41.0	41.0	32.0	41.0
60-61	38.93975479132436	41.0	41.0	41.0	37.0	41.0
62-63	38.93669739916625	41.0	41.0	41.0	37.0	41.0
64-65	38.91711789575352	41.0	41.0	41.0	34.5	41.0
66-67	38.87585671402092	41.0	41.0	41.0	32.0	41.0
68-69	38.85861146530149	41.0	41.0	41.0	32.0	41.0
70-71	38.832362040483815	41.0	41.0	41.0	32.0	41.0
72-73	38.80249649274518	41.0	41.0	41.0	32.0	41.0
74-75	38.78315600755437	41.0	41.0	41.0	32.0	41.0
76-77	37.89222470177094	41.0	39.0	41.0	32.0	41.0
78-79	38.35896649230243	41.0	39.0	41.0	32.0	41.0
80-81	38.703280128391555	41.0	41.0	41.0	32.0	41.0
82-83	38.86727570471872	41.0	41.0	41.0	32.0	41.0
84-85	38.82240852769225	41.0	41.0	41.0	32.0	41.0
86-87	38.75960978797354	41.0	41.0	41.0	32.0	41.0
88-89	38.70285137662144	41.0	41.0	41.0	32.0	41.0
90-91	38.64759361202178	41.0	41.0	41.0	32.0	41.0
92-93	38.57828142351437	41.0	41.0	41.0	32.0	41.0
94-95	38.56731609699361	41.0	41.0	41.0	32.0	41.0
96-97	38.47086309691511	41.0	37.0	41.0	32.0	41.0
98-99	38.26309008245818	41.0	37.0	41.0	32.0	41.0
100-101	37.26791828476641	41.0	37.0	41.0	29.5	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	33.0
13	97.0
14	350.0
15	1340.0
16	6158.0
17	16763.0
18	29797.0
19	41395.0
20	51901.0
21	63250.0
22	76180.0
23	90656.0
24	107526.0
25	126912.0
26	147757.0
27	173297.0
28	201114.0
29	235300.0
30	274654.0
31	324079.0
32	383878.0
33	459651.0
34	560981.0
35	704961.0
36	925853.0
37	1321020.0
38	2256324.0
39	5878752.0
40	1.767983E7
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.737338492054313	17.47517925561408	14.41175885576475	41.375723396566855
2	27.97815008794856	19.27673558981013	35.480823797054924	17.264290525186382
3	21.116409329448423	22.52564310191108	30.445661733054113	25.912285835586385
4	23.91884780646954	29.137416466911798	23.685735033459597	23.25800069315907
5	27.14420080419734	31.355599598060024	22.987228140453208	18.512971457289428
6	20.688470799562	35.25714480755004	23.15126701593031	20.903117376957653
7	20.84393843161918	18.086190244627776	38.79281609918715	22.277055224565895
8	22.062374421702383	23.0167640386413	27.669915524389083	27.250946015267235
9	22.921234535027885	22.03886152528162	29.8898602664378	25.150043673252693
10-11	25.47462629084961	28.00115004064984	22.777242381303502	23.746981287197045
12-13	26.214979851139365	22.231711018335805	25.52531477582439	26.02799435470044
14-15	24.339131884697885	26.059040985588933	26.539703456233983	23.062123673479203
16-17	25.454252385160085	25.609270561105003	25.6035361767313	23.332940877003615
18-19	25.107265367126345	26.46229711351195	25.432506962823243	22.997930556538456
20-21	25.472554407417096	26.108752356300226	25.440366468936165	22.978326767346513
22-23	25.016743314878582	26.003124858722202	25.84657855941561	23.133553266983604
24-25	24.67893732687915	25.98861362760017	26.173130143160815	23.159318902359864
26-27	24.92248164884863	26.167639950816135	25.945998932352087	22.963879467983148
28-29	25.075033196316465	26.033059162687405	25.8651033500992	23.026804290896933
30-31	24.73842330550253	26.027933146709113	25.99457887257513	23.23906467521322
32-33	24.99852784621856	26.317375383407576	25.763330599470507	22.920766170903356
34-35	25.040554845861095	26.21764180365851	25.665443438073947	23.07635991240645
36-37	24.830170894917266	26.128594914798654	25.974084040138507	23.067150150145572
38-39	25.146069367569186	26.251326013473964	25.809120054137182	22.793484564819675
40-41	25.23517019033934	26.00846352260525	25.628688396997006	23.1276778900584
42-43	24.761726076756094	26.091711197105933	26.02767012868031	23.118892597457663
44-45	25.006944331979064	26.11225173692308	26.048129747299747	22.832674183798108
46-47	25.192698386636252	25.96764261726394	25.84227266693167	22.997386329168133
48-49	24.909351880295663	25.999357112816124	26.07951732246791	23.0117736844203
50-51	25.16964553211875	26.05541464169871	25.793544697169796	22.981395129012746
52-53	25.329206421571033	25.92167872343219	25.69761467773441	23.051500177262362
54-55	24.73615116524637	25.988393624232987	26.14033516997884	23.135120040541807
56-57	24.958108546684137	26.174574400772176	25.99088006488623	22.876436987657456
58-59	25.258841768474728	25.88124434715838	25.835046188357868	23.02486769600902
60-61	25.06408759643942	26.03771376980743	25.852200789242634	23.04599784451051
62-63	25.22939592127362	26.013332562509106	25.908404736735623	22.848866779481646
64-65	25.188497542098027	26.011769391660046	25.781147299288556	23.018585766953375
66-67	24.86865236865855	26.192023966610954	26.0683986399036	22.87092502482689
68-69	25.1571989680172	26.3435765685679	25.798785880993286	22.70043858242161
70-71	25.462455494164573	26.020363451416443	25.632078580403345	22.885102474015643
72-73	24.989415774063872	26.170376432541957	25.944440117861312	22.89576767553286
74-75	25.00112672060582	26.349717554440826	25.838821905601677	22.810333819351676
76-77	25.38605306013692	26.049709657531256	25.669085802189567	22.895151480142257
78-79	24.969687903247966	26.280394198982325	25.86550996616066	22.88440793160905
80-81	25.29547843883565	26.27676529833171	25.83206363393452	22.595692628898117
82-83	25.448275632465567	26.030262626737827	25.7612468418026	22.760214898994
84-85	25.194348779495122	26.186609459209343	25.919007237747948	22.700034523547586
86-87	25.25391174539961	26.413307247718866	25.621823079284635	22.710957927596894
88-89	25.434153289986757	26.089174706245664	25.73675305018514	22.739918953582436
90-91	25.056427701990298	26.301945758901603	26.02332547257063	22.618301066537473
92-93	25.441043281256633	26.379868906264125	25.70634310562168	22.472744706857565
94-95	25.64369626465422	26.169682588966225	25.62869150840318	22.55792963797638
96-97	25.373113407144153	26.23405656709482	25.907128789145656	22.48570123661537
98-99	25.607304214143817	26.51297094933922	25.529011884873903	22.35071295164306
100-101	25.713686585239063	26.219945073525626	25.619809922120623	22.44655841911469
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	19.0
1	18.5
2	18.5
3	19.5
4	22.0
5	25.5
6	44.0
7	64.5
8	70.5
9	89.5
10	119.0
11	155.5
12	184.5
13	204.0
14	256.5
15	345.5
16	427.0
17	523.5
18	688.5
19	868.5
20	1072.0
21	1437.5
22	1888.0
23	2492.0
24	3322.0
25	4381.0
26	5890.0
27	8032.5
28	11202.0
29	15632.0
30	21689.5
31	30646.0
32	44336.0
33	64688.5
34	97199.0
35	149472.0
36	226616.0
37	335530.0
38	488074.0
39	694642.0
40	932364.0
41	1172658.0
42	1420529.0
43	1633991.5
44	1810849.5
45	1969168.0
46	2039155.5
47	2043491.5
48	2001855.5
49	1897963.0
50	1740853.5
51	1591730.5
52	1441853.5
53	1250622.0
54	1083470.0
55	944970.5
56	812163.5
57	689951.0
58	571958.5
59	449632.0
60	373704.0
61	345097.0
62	314815.0
63	266374.5
64	214743.0
65	184663.5
66	166301.5
67	141905.0
68	106619.0
69	76850.0
70	63928.5
71	52774.0
72	40021.0
73	29225.5
74	19570.5
75	11782.0
76	6713.0
77	3974.5
78	2577.5
79	1668.0
80	961.0
81	652.5
82	463.0
83	331.5
84	263.0
85	220.0
86	191.0
87	148.0
88	119.5
89	108.5
90	87.5
91	75.5
92	66.0
93	50.0
94	42.0
95	40.5
96	40.0
97	42.0
98	45.0
99	85.0
100	452.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04280672607606349
2	0.0
3	2.3024405652192896E-4
4	0.0
5	0.008313677284143164
6	0.0
7	0.0
8	0.0
9	0.0
10-11	2.349111657757518E-4
12-13	4.68266628466896E-4
14-15	0.0
16-17	0.0010952149715637701
18-19	2.6446952438329675E-5
20-21	0.0013721301206239278
22-23	3.609231156289697E-4
24-25	0.0029605029700083157
26-27	0.0
28-29	0.002181095724619894
30-31	0.0
32-33	0.004737115892630227
34-35	0.0
36-37	0.0
38-39	0.0022168768955658697
40-41	0.0
42-43	8.603038057880181E-4
44-45	8.976406798186013E-4
46-47	0.0016443781604302628
48-49	0.001313013403408838
50-51	0.0
52-53	0.0031331860123997627
54-55	4.55821003790035E-4
56-57	0.0034023226460368826
58-59	0.0
60-61	2.955835860754493E-5
62-63	0.0032700878838452336
64-65	0.0
66-67	0.002375558610195848
68-69	3.920371773211223E-4
70-71	0.002817378286224414
72-73	0.0
74-75	2.349111657757518E-4
76-77	0.003498776237282555
78-79	0.0
80-81	0.004033938098387579
82-83	0.0013410160589317753
84-85	0.0027240361011479563
86-87	0.0
88-89	0.0010127627080795656
90-91	3.1114061692152554E-5
92-93	4.1848412975945194E-4
94-95	0.0
96-97	0.0023444445485036954
98-99	2.0224140099899162E-5
100-101	9.287547415107538E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3.2139809E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.585692846576
#Duplication Level	Percentage of deduplicated	Percentage of total
1	63.230937213643244	17.442692123766946
2	14.443727634887962	7.968804681912419
3	6.787497143535043	5.617134341957089
4	3.750576951331528	4.138490551075159
5	2.3208183779886338	3.2010691463941585
6	1.6360798856119987	2.7079438318172273
7	1.1888747735791567	2.295715403589804
8	0.9069502594841328	2.0015081028201354
9	0.7393578432278078	1.8356128530290277
>10	4.340197035379271	22.315306354161454
>50	0.3694871756798321	7.070834737364096
>100	0.2504027404279022	13.559660061331483
>500	0.023999621107423832	4.527126054745087
>1k	0.010963911237590634	5.047922351562971
>5k	1.1766625322935127E-4	0.21417455753245293
>10k+	1.1766625322935126E-5	0.056004846940347415
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.9782498707444094E-5	0.0	0.0	3.111406169215256E-6	3.111406169215256E-6
2	5.289390487665935E-5	6.222812338430512E-6	6.222812338430512E-6	3.111406169215256E-6	3.111406169215256E-6
3	7.77851542303814E-5	6.222812338430512E-6	6.222812338430512E-6	3.111406169215256E-6	3.111406169215256E-6
4	1.400132776146865E-4	9.334218507645767E-6	4.0448280199798324E-5	6.222812338430512E-6	3.111406169215256E-6
5	1.6801593313762382E-4	9.334218507645767E-6	5.289390487665935E-5	6.222812338430512E-6	3.111406169215256E-6
6	2.2713265035271367E-4	1.2445624676861023E-5	1.0267640358410344E-4	6.222812338430512E-6	3.111406169215256E-6
7	2.5513530587565096E-4	1.2445624676861023E-5	1.0889921592253395E-4	1.2445624676861023E-5	3.111406169215256E-6
8	2.8313796139858827E-4	1.2445624676861023E-5	1.0889921592253395E-4	1.2445624676861023E-5	6.222812338430512E-6
9	3.1425202309074085E-4	1.555703084607628E-5	1.1512202826096446E-4	1.2445624676861023E-5	6.222812338430512E-6
10-11	3.6559022488279256E-4	1.555703084607628E-5	1.2134484059939498E-4	1.2445624676861023E-5	7.778515423038138E-6
12-13	4.542653007054274E-4	1.555703084607628E-5	1.353461683608636E-4	1.2445624676861023E-5	1.0889921592253395E-5
14-15	5.491631888664926E-4	1.8668437015291533E-5	1.4156898069929414E-4	1.8668437015291533E-5	1.2445624676861023E-5
16-17	6.6895232638128E-4	1.8668437015291533E-5	2.0379710408359924E-4	2.0224140099899162E-5	1.2445624676861023E-5
18-19	7.965199793191055E-4	1.8668437015291533E-5	2.1624272876046027E-4	2.333554626911442E-5	1.2445624676861023E-5
20-21	9.692030217105521E-4	1.8668437015291533E-5	2.3802257194496706E-4	2.4891249353722047E-5	1.4001327761468652E-5
22-23	0.0011714444227095438	2.177984318450679E-5	2.8313796139858827E-4	2.4891249353722047E-5	1.8668437015291533E-5
24-25	0.0015619258969460585	2.6446952438329675E-5	2.8624936756780354E-4	2.4891249353722047E-5	2.177984318450679E-5
26-27	0.002093976351881867	3.111406169215256E-5	3.002506953292722E-4	2.4891249353722047E-5	2.4891249353722047E-5
28-29	0.0026540294623406133	3.111406169215256E-5	3.329204601060324E-4	3.2669764776760185E-5	2.6446952438329672E-5
30-31	0.0033758756935985523	3.422546786136781E-5	3.453660847828934E-4	3.422546786136781E-5	2.955835860754493E-5
32-33	0.004186397000679127	3.733687403058307E-5	3.562560063751468E-4	3.422546786136781E-5	3.111406169215256E-5
34-35	0.005164934240897324	4.200398328440595E-5	4.7293373772071886E-4	3.422546786136781E-5	3.111406169215256E-5
36-37	0.006376826943806667	4.667109253822884E-5	5.05603502497479E-4	3.422546786136781E-5	3.111406169215256E-5
38-39	0.008140994241751718	4.667109253822884E-5	5.149377210051248E-4	3.422546786136781E-5	3.111406169215256E-5
40-41	0.010404542229855814	4.9782498707444094E-5	5.476074857818851E-4	3.733687403058307E-5	3.2669764776760185E-5
42-43	0.013295038561056786	4.9782498707444094E-5	5.865000628970758E-4	3.733687403058307E-5	3.422546786136781E-5
44-45	0.016614908943609466	5.756101413048224E-5	5.927228752355062E-4	3.733687403058307E-5	3.88925771151907E-5
46-47	0.020171246195022502	6.222812338430512E-5	6.51839592450596E-4	3.88925771151907E-5	4.355968636901358E-5
48-49	0.024752791779191964	6.222812338430512E-5	6.81397951058141E-4	4.0448280199798324E-5	4.355968636901358E-5
50-51	0.031187179737129117	6.533952955352037E-5	6.985106849888248E-4	4.0448280199798324E-5	4.511538945362121E-5
52-53	0.0391165983593742	6.845093572273562E-5	7.389589651886232E-4	4.0448280199798324E-5	4.9782498707444094E-5
54-55	0.04806500250203727	6.845093572273562E-5	8.260783379266505E-4	4.0448280199798324E-5	5.4449607961266975E-5
56-57	0.057371218354160106	7.000663880734325E-5	9.038634921570318E-4	4.200398328440595E-5	5.60053110458746E-5
58-59	0.0676497486341627	7.156234189195088E-5	9.209762260877157E-4	4.355968636901358E-5	5.60053110458746E-5
60-61	0.08018715979301558	7.156234189195088E-5	9.583131001182988E-4	4.355968636901358E-5	5.60053110458746E-5
62-63	0.0953677104926168	7.467374806116613E-5	0.0010252083327564267	4.355968636901358E-5	5.60053110458746E-5
64-65	0.11377790079586347	7.77851542303814E-5	0.0010594338006177945	4.355968636901358E-5	5.911671721508986E-5
66-67	0.13534772406394824	7.77851542303814E-5	0.0010812136438023015	4.355968636901358E-5	6.222812338430512E-5
68-69	0.1600413991259251	8.245226348420428E-5	0.0010889921592253395	4.355968636901358E-5	6.222812338430512E-5
70-71	0.1885901064315597	8.556366965341953E-5	0.0010905478623099473	4.355968636901358E-5	6.222812338430512E-5
72-73	0.22273623343561252	9.023077890724241E-5	0.0011014377839022006	4.355968636901358E-5	6.222812338430512E-5
74-75	0.2654760642790379	9.334218507645767E-5	0.0011045491900714159	4.355968636901358E-5	6.222812338430512E-5
76-77	0.3184057503266432	9.489788816106529E-5	0.0011107720024098463	4.355968636901358E-5	6.533952955352037E-5
78-79	0.38317433684811253	1.0578780975331869E-4	0.0011185505178328845	4.355968636901358E-5	7.000663880734325E-5
80-81	0.4595017972882166	1.1356632517635683E-4	0.0011247733301713151	4.355968636901358E-5	7.77851542303814E-5
82-83	0.5476059300788004	1.1512202826096446E-4	0.0011465531733558217	4.355968636901358E-5	7.77851542303814E-5
84-85	0.6541015847356155	1.1667773134557208E-4	0.0011558873918634674	4.355968636901358E-5	7.77851542303814E-5
86-87	0.7883960978112844	1.229005436840026E-4	0.0011652216103711132	4.355968636901358E-5	7.77851542303814E-5
88-89	0.9515986856051323	1.3067905910704074E-4	0.0011698887196249363	4.355968636901358E-5	7.934085731498903E-5
90	1.0937370536333928	1.555703084607628E-4	0.0011730001257941514	4.355968636901358E-5	8.089656039959665E-5
>>END_MODULE
