##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	SRR19654117_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	38275839
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.417980543809897	32.0	32.0	32.0	32.0	32.0
2	31.48964815637353	32.0	32.0	32.0	32.0	32.0
3	34.86727797135943	37.0	32.0	37.0	32.0	37.0
4	35.80707579003036	37.0	37.0	37.0	32.0	37.0
5	35.97041682090887	37.0	37.0	37.0	37.0	37.0
6	39.418522661253746	41.0	41.0	41.0	37.0	41.0
7	39.536867395643505	41.0	41.0	41.0	37.0	41.0
8	39.663047020340954	41.0	41.0	41.0	37.0	41.0
9	39.5910763445316	41.0	41.0	41.0	37.0	41.0
10-11	39.58892765224559	41.0	41.0	41.0	37.0	41.0
12-13	39.69144912799952	41.0	41.0	41.0	37.0	41.0
14-15	39.61528493209515	41.0	41.0	41.0	37.0	41.0
16-17	39.55028524652327	41.0	41.0	41.0	37.0	41.0
18-19	39.57965846287523	41.0	41.0	41.0	37.0	41.0
20-21	39.62604922912337	41.0	41.0	41.0	37.0	41.0
22-23	39.41434757315183	41.0	41.0	41.0	37.0	41.0
24-25	39.44200193756693	41.0	41.0	41.0	37.0	41.0
26-27	39.47803597460006	41.0	41.0	41.0	37.0	41.0
28-29	39.34046310519803	41.0	41.0	41.0	37.0	41.0
30-31	39.390995583401846	41.0	41.0	41.0	37.0	41.0
32-33	39.50833148294934	41.0	41.0	41.0	37.0	41.0
34-35	39.467058827894014	41.0	41.0	41.0	37.0	41.0
36-37	39.29831035186453	41.0	41.0	41.0	37.0	41.0
38-39	39.21792377954145	41.0	41.0	41.0	37.0	41.0
40-41	39.141980440977406	41.0	41.0	41.0	37.0	41.0
42-43	39.12957945872852	41.0	41.0	41.0	37.0	41.0
44-45	39.13987407304122	41.0	41.0	41.0	37.0	41.0
46-47	39.08716473857046	41.0	41.0	41.0	37.0	41.0
48-49	39.039415830963236	41.0	41.0	41.0	37.0	41.0
50-51	39.08939902009725	41.0	41.0	41.0	37.0	41.0
52-53	39.090630776245035	41.0	41.0	41.0	37.0	41.0
54-55	39.08294511845972	41.0	41.0	41.0	37.0	41.0
56-57	39.04643832888941	41.0	41.0	41.0	37.0	41.0
58-59	38.95313185688758	41.0	41.0	41.0	37.0	41.0
60-61	38.994806436509464	41.0	41.0	41.0	37.0	41.0
62-63	38.99037109284528	41.0	41.0	41.0	37.0	41.0
64-65	38.967431399740185	41.0	41.0	41.0	37.0	41.0
66-67	38.93162263536536	41.0	41.0	41.0	37.0	41.0
68-69	38.91349882624389	41.0	41.0	41.0	37.0	41.0
70-71	38.88685593541137	41.0	41.0	41.0	32.0	41.0
72-73	38.85921543091453	41.0	41.0	41.0	32.0	41.0
74-75	38.83674638719219	41.0	41.0	41.0	32.0	41.0
76-77	37.965846013199084	41.0	39.0	41.0	32.0	41.0
78-79	38.42242100819789	41.0	39.0	41.0	32.0	41.0
80-81	38.762574910506856	41.0	41.0	41.0	32.0	41.0
82-83	38.928987448191535	41.0	41.0	41.0	34.5	41.0
84-85	38.88810754742698	41.0	41.0	41.0	32.0	41.0
86-87	38.82449672755703	41.0	41.0	41.0	32.0	41.0
88-89	38.76834007479235	41.0	41.0	41.0	32.0	41.0
90-91	38.71714661826224	41.0	41.0	41.0	32.0	41.0
92-93	38.65287408853402	41.0	41.0	41.0	32.0	41.0
94-95	38.63859129253836	41.0	41.0	41.0	32.0	41.0
96-97	38.54066369387749	41.0	41.0	41.0	32.0	41.0
98-99	38.33952705778702	41.0	37.0	41.0	32.0	41.0
100-101	37.36471410855292	41.0	37.0	41.0	29.5	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	22.0
13	119.0
14	401.0
15	1677.0
16	7607.0
17	21111.0
18	37178.0
19	50152.0
20	63328.0
21	74954.0
22	91597.0
23	107777.0
24	126188.0
25	146330.0
26	169401.0
27	196704.0
28	228744.0
29	265568.0
30	311033.0
31	368790.0
32	439570.0
33	523529.0
34	642861.0
35	808073.0
36	1060013.0
37	1488701.0
38	2472818.0
39	6297941.0
40	2.2273652E7
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	31.882495570523012	21.539728229226466	12.309233479621689	34.26854272062883
2	28.910156613418714	25.181728870789744	29.34429471291276	16.563819802878783
3	21.27520121626936	27.583631842756134	29.160317561007066	21.98084937996744
4	24.793047645539527	30.402338143391187	23.84539238970046	20.95922182136883
5	27.989580126080693	32.04817740258888	22.130717323020797	17.83152514830963
6	22.641147069303955	37.33594709707082	21.77967934288782	18.243226490737406
7	22.480549675214174	22.743527581459418	35.18373561974696	19.592187123579446
8	23.12248204409053	25.068602676482154	27.54162750031423	24.267287779113083
9	23.717993484087966	23.37493634038956	29.810317155947907	23.096753019574567
10-11	24.996088255845443	30.272739160144805	23.70020298745172	21.03096959655803
12-13	25.75987660888029	25.23852216581972	26.00418051270561	22.997420712594376
14-15	24.730031391343243	26.975143510244155	26.153290852749173	22.141534245663433
16-17	25.563339354295888	26.40015008056798	25.708958837222035	22.3275517279141
18-19	25.179494822391224	26.9226825689233	25.794608923588076	22.1032136850974
20-21	25.459784817862673	26.928448835509982	25.565848008072884	22.045918338554454
22-23	25.41749070990259	26.735665096357998	25.64546432094178	22.20137987279763
24-25	24.80129700257444	26.9095519255638	26.067258193869623	22.221892877992133
26-27	25.123591673588137	26.97866662047565	25.918171251582496	21.979570454353727
28-29	25.340872265606134	26.594732340266606	25.79678207483947	22.267613319287786
30-31	24.932840792856297	26.79058975036445	26.041537587196977	22.23503186958227
32-33	25.059228477628675	26.978550821255965	25.847467928612012	22.114752772503348
34-35	25.258021123978498	26.689870860832077	25.8036329393067	22.248475075882727
36-37	24.89481811228227	26.705725510027357	26.191931416578484	22.20752496111189
38-39	25.12076922348389	26.951413734239456	25.86074120908675	22.067075833189907
40-41	25.351104648548656	26.571869528451092	25.768800260655293	22.308225562344955
42-43	24.885708897710774	26.69448698107416	26.20134003185536	22.218464089359703
44-45	25.10196403146273	26.75351106387696	26.028446860285875	22.116078044374436
46-47	25.29214876309438	26.64440847220309	25.872779423288065	22.19066334141446
48-49	24.833919781590904	26.73322154853326	26.17981415652887	22.253044513346964
50-51	25.148335742555506	26.768740457916547	25.85630977285697	22.226614026670976
52-53	25.412614849362487	26.556715553205695	25.817470319418888	22.213199278012926
54-55	24.920667261222142	26.70718406657161	26.183697263752865	22.188451408453382
56-57	24.969718124670067	26.89972408555085	26.003109899804166	22.127447889974917
58-59	25.227324213585494	26.52091571395731	25.947763026174293	22.303997046282905
60-61	25.03193791297933	26.573154815783727	26.153836627849653	22.241070643387292
62-63	25.146294155721765	26.687956330723576	26.039710462798325	22.126039050756326
64-65	25.30686002728771	26.511080527849433	25.984795525971354	22.197263918891498
66-67	24.958171062223535	26.697162775286532	26.252131355509967	22.09253480697996
68-69	25.19263305198633	26.881041653399436	25.94918902501751	21.977136269596727
70-71	25.49750635291336	26.604470765821215	25.73213746264265	22.16588541862278
72-73	24.967745318397856	26.785717224905248	26.14032967376626	22.106207782930635
74-75	24.99639556400622	26.850837223905653	26.0126904102016	22.140076801886522
76-77	25.380862103923956	26.574069892537246	25.856772765680073	22.18829523785873
78-79	24.95853715969492	26.7904983088679	26.188634297474184	22.062330233962996
80-81	25.25100595789495	26.871441455666623	25.931247593933072	21.946304992505357
82-83	25.46244385008121	26.57274148375889	25.878325388375238	22.08648927778466
84-85	25.164922857770982	26.75052273639605	26.13707845831728	21.947475947515688
86-87	25.25936792659202	26.92385136221312	25.91679962913419	21.89998108206067
88-89	25.407659768159245	26.738323296417636	25.790731649985542	22.063285285437573
90-91	24.991718664016396	26.885209984609347	26.104051201608204	22.019020149766057
92-93	25.341256010226545	26.929859243983252	25.85493963343505	21.873945112355166
94-95	25.68825205895552	26.651737144155092	25.736608934947185	21.923401861942203
96-97	25.233658875481414	26.783076835090007	26.047899615521086	21.935364673907493
98-99	25.463483375221603	27.058037808634527	25.736704074648042	21.74177474149583
100-101	25.792542232383518	26.72008558578395	25.68623144997224	21.801140731860293
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	22.0
1	22.5
2	22.0
3	22.0
4	28.5
5	47.0
6	65.5
7	80.5
8	100.5
9	120.0
10	159.5
11	204.5
12	243.0
13	321.5
14	425.5
15	511.0
16	651.0
17	849.5
18	1068.0
19	1355.5
20	1794.5
21	2357.5
22	3134.0
23	4228.0
24	5694.5
25	7863.5
26	10741.0
27	14525.5
28	20217.0
29	27820.5
30	38452.0
31	54949.5
32	79862.0
33	114082.0
34	168637.5
35	256203.5
36	377114.5
37	537735.5
38	754360.5
39	1019572.5
40	1301291.0
41	1587121.0
42	1880188.5
43	2126924.5
44	2312887.5
45	2428997.5
46	2457776.0
47	2429723.0
48	2333366.0
49	2168364.5
50	1957339.0
51	1762728.0
52	1584700.5
53	1401658.0
54	1218947.0
55	1038783.5
56	860219.0
57	687049.5
58	545739.0
59	446151.5
60	380550.5
61	324744.5
62	272012.5
63	226518.0
64	197916.5
65	173524.0
66	148623.5
67	126184.5
68	99376.5
69	75497.5
70	61516.0
71	49740.5
72	36743.0
73	25279.5
74	15667.5
75	9353.5
76	5589.0
77	3128.5
78	1819.0
79	1165.0
80	782.5
81	575.5
82	464.5
83	373.5
84	311.0
85	271.5
86	227.5
87	188.5
88	158.5
89	137.5
90	122.0
91	93.0
92	63.0
93	59.5
94	63.5
95	65.5
96	58.5
97	58.0
98	72.0
99	109.5
100	547.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.042436692243375776
2	0.0
3	2.795497180349201E-4
4	0.0
5	0.008378653698485877
6	0.0
7	0.0
8	0.0
9	0.0
10-11	1.933334498559261E-4
12-13	3.945047422735789E-4
14-15	0.0
16-17	0.0010385141394287922
18-19	3.52702915277703E-5
20-21	0.0014186495036725387
22-23	3.579281436521875E-4
24-25	0.0029444161890220095
26-27	0.0
28-29	0.002180226539253653
30-31	0.0
32-33	0.004727525371814841
34-35	0.0
36-37	0.0
38-39	0.0022899563351178273
40-41	0.0
42-43	8.647752959771829E-4
44-45	8.673879101644251E-4
46-47	0.001690361379145732
48-49	0.0013324332354935446
50-51	0.0
52-53	0.0031155424182863762
54-55	4.8202731754619414E-4
56-57	0.003380722758291464
58-59	0.0
60-61	3.3963984434149175E-5
62-63	0.0032422542063676256
64-65	0.0
66-67	0.002353965382705262
68-69	3.9581104936720003E-4
70-71	0.002812479172566276
72-73	0.0
74-75	2.2599112719645415E-4
76-77	0.0034577948768151102
78-79	0.0
80-81	0.003915002359582503
82-83	0.0013494152277106193
84-85	0.0027445512036979778
86-87	0.0
88-89	0.001007162769181885
90-91	2.6126141872422444E-5
92-93	3.644596791202931E-4
94-95	0.0
96-97	0.0023983798238883802
98-99	1.4369378029832343E-5
100-101	8.45180689572866E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3.8275839E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.00170631641832
#Duplication Level	Percentage of deduplicated	Percentage of total
1	60.657475900478	18.198277778613626
2	15.516921179028564	9.31068224296453
3	7.071344994945488	6.364572474012873
4	4.193927320705901	5.032999031528863
5	2.629174519283732	3.9439860891080416
6	1.82398602518422	3.2833615831696887
7	1.2890760702528796	2.7072137175474666
8	0.9659213616602415	2.3183431213828363
9	0.7783782352188573	2.101740769451532
>10	4.554068734504941	25.046158690049648
>50	0.3263556444614238	6.760025829081777
>100	0.17713943269326854	9.827417124297591
>500	0.0111909836675415	2.2938885112681406
>1k	0.004874466453782231	2.4817862072155226
>5k	1.559574912616021E-4	0.2943222862453141
>10k+	9.173970074211887E-6	0.03522454406291233
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.6126141872422444E-5	0.0	1.828829931069571E-5	0.0	0.0
2	3.657659862139142E-5	1.0450456748968977E-5	3.3963984434149175E-5	5.225228374484489E-6	0.0
3	5.486489793208713E-5	1.3063070936211222E-5	4.180182699587591E-5	5.225228374484489E-6	0.0
4	8.360365399175182E-5	1.828829931069571E-5	6.009012630657162E-5	5.225228374484489E-6	0.0
5	1.0450456748968978E-4	2.873875605966469E-5	7.05405830555406E-5	1.3063070936211222E-5	0.0
6	1.410811661110812E-4	2.873875605966469E-5	7.837842561726733E-5	1.3063070936211222E-5	0.0
7	1.5153162286005018E-4	2.873875605966469E-5	9.927933911520529E-5	1.3063070936211222E-5	0.0
8	1.8288299310695712E-4	2.873875605966469E-5	1.227928668003855E-4	1.828829931069571E-5	5.225228374484489E-6
9	2.0117129241765282E-4	3.657659862139142E-5	1.3846855192383895E-4	1.828829931069571E-5	5.225228374484489E-6
10-11	2.429731194135287E-4	5.094597665122377E-5	1.6459469379626138E-4	2.35135276851802E-5	5.225228374484489E-6
12-13	3.2657677340528055E-4	5.486489793208713E-5	1.8680191438782048E-4	2.6126141872422444E-5	5.225228374484489E-6
14-15	3.9842366355444226E-4	6.009012630657162E-5	2.1423436335386403E-4	2.7432448966043566E-5	7.837842561726733E-6
16-17	5.068471523249954E-4	6.400904758743499E-5	2.8608125350302574E-4	2.873875605966469E-5	7.837842561726733E-6
18-19	6.335589404062442E-4	6.792796886829835E-5	3.2918938759252277E-4	3.52702915277703E-5	9.144149655347855E-6
20-21	7.890094845471578E-4	8.621626817899407E-5	3.644596791202931E-4	3.657659862139142E-5	1.0450456748968977E-5
22-23	9.914870840584318E-4	9.144149655347856E-5	4.245498054268647E-4	3.657659862139142E-5	1.0450456748968977E-5
24-25	0.0013716224483021782	1.0842348877055314E-4	4.5982009695463497E-4	3.9189212808633664E-5	1.17567638425901E-5
26-27	0.0019307218843720186	1.1756763842590099E-4	4.833336246398152E-4	3.9189212808633664E-5	1.5675685123453465E-5
28-29	0.0025930195808379275	1.2671178808124886E-4	5.186039161675854E-4	4.049551990225479E-5	1.5675685123453465E-5
30-31	0.00337549752991698	1.5414423704729243E-4	5.251354516356911E-4	4.572074827673928E-5	1.6981992217074587E-5
32-33	0.004314732330230567	1.8027037891971484E-4	5.329732941974179E-4	4.70270553703604E-5	1.828829931069571E-5
34-35	0.005362390619314707	1.9202714276230496E-4	6.400904758743499E-4	4.70270553703604E-5	1.9594606404316832E-5
36-37	0.0067183373824934315	1.946397569495472E-4	6.923427596191948E-4	5.094597665122377E-5	2.35135276851802E-5
38-39	0.008314644650898442	2.0900913497937955E-4	7.236941298661017E-4	5.486489793208713E-5	2.481983477880132E-5
40-41	0.010443925213500873	2.1815328463472742E-4	7.537391930193875E-4	5.486489793208713E-5	2.6126141872422444E-5
42-43	0.013077440314241054	2.24684820102833E-4	8.216671618876859E-4	5.486489793208713E-5	3.657659862139142E-5
44-45	0.0161603250551869	2.416668123199076E-4	8.517122250409717E-4	5.617120502570826E-5	4.049551990225479E-5
46-47	0.019398660340273665	2.442794265071499E-4	9.118023513475433E-4	5.747751211932938E-5	4.4414441183118154E-5
48-49	0.023547491669614347	2.521172690688766E-4	9.379284932199657E-4	5.747751211932938E-5	4.963966955760264E-5
50-51	0.02875965697316263	2.8608125350302574E-4	9.61442020905146E-4	5.747751211932938E-5	5.225228374484489E-5
52-53	0.035208895094370106	3.082884740945848E-4	9.901807769648106E-4	6.009012630657162E-5	5.486489793208713E-5
54-55	0.0423269624475116	3.082884740945848E-4	0.0010254510684925809	6.270274049381386E-5	5.747751211932938E-5
56-57	0.05031241771081752	3.148200095626904E-4	0.0010763970451438047	6.400904758743499E-5	6.139643340019273E-5
58-59	0.05966949542242562	3.174326237499327E-4	0.0010986042657353638	6.531535468105611E-5	6.270274049381386E-5
60-61	0.07116369153919787	3.2004523793717493E-4	0.0011678385416972832	6.531535468105611E-5	6.270274049381386E-5
62-63	0.08599942120145296	3.2918938759252277E-4	0.001260586345344383	6.662166177467724E-5	6.270274049381386E-5
64-65	0.1038135310371642	3.344146159670073E-4	0.0013206764716509546	6.792796886829835E-5	7.707211852364622E-5
66-67	0.1242964262651434	3.4617137980959734E-4	0.0013298206213063024	6.792796886829835E-5	8.882888236623631E-5
68-69	0.14866819771083267	3.6054075783942973E-4	0.0013468026135233769	6.792796886829835E-5	9.40541107407208E-5
70-71	0.1771692058794583	3.840542855246099E-4	0.001358559377365967	6.792796886829835E-5	1.1364871714503763E-4
72-73	0.21307697526891572	3.9058582099271553E-4	0.0013990548972682218	6.923427596191948E-5	1.2932440226849108E-4
74-75	0.25717267752119033	3.945047422735789E-4	0.0014055864327363276	7.837842561726733E-5	1.3846855192383895E-4
76-77	0.3118350978537662	3.9972997064806337E-4	0.0014277936533278867	8.099103980450958E-5	1.3846855192383895E-4
78-79	0.37587680311854166	4.101804273970324E-4	0.0014460819526385823	8.360365399175182E-5	1.3846855192383895E-4
80-81	0.45199009223547004	4.219371912396225E-4	0.0014591450235747934	8.360365399175182E-5	1.3846855192383895E-4
82-83	0.5430357777395813	4.350002621758337E-4	0.0014787396299791103	8.360365399175182E-5	1.410811661110812E-4
84-85	0.6546623315036935	4.3891918345669706E-4	0.0014944153151025639	8.360365399175182E-5	1.410811661110812E-4
86-87	0.7930172347103874	4.467570260184238E-4	0.001507478386038775	8.360365399175182E-5	1.410811661110812E-4
88-89	0.9581788657852803	4.49369640205666E-4	0.001508784693132396	8.621626817899407E-5	1.410811661110812E-4
90	1.0996806627805076	4.6243271114187724E-4	0.0015100910002260172	8.621626817899407E-5	1.4630639448556568E-4
>>END_MODULE
