##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	SRR19654115_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	30751883
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.611829786163014	32.0	32.0	32.0	32.0	32.0
2	31.60457380772423	32.0	32.0	32.0	32.0	32.0
3	35.69164353285293	37.0	37.0	37.0	32.0	37.0
4	36.32081833818111	37.0	37.0	37.0	37.0	37.0
5	36.50801094033819	37.0	37.0	37.0	37.0	37.0
6	40.06502544901072	41.0	41.0	41.0	37.0	41.0
7	40.09264141646221	41.0	41.0	41.0	37.0	41.0
8	40.2016592609955	41.0	41.0	41.0	37.0	41.0
9	40.21318031159262	41.0	41.0	41.0	37.0	41.0
10-11	40.2715400224435	41.0	41.0	41.0	41.0	41.0
12-13	40.25472230432199	41.0	41.0	41.0	41.0	41.0
14-15	40.20171172932727	41.0	41.0	41.0	41.0	41.0
16-17	40.223070551484604	41.0	41.0	41.0	41.0	41.0
18-19	40.211025630528056	41.0	41.0	41.0	41.0	41.0
20-21	40.18568054840739	41.0	41.0	41.0	41.0	41.0
22-23	40.17047843866992	41.0	41.0	41.0	39.0	41.0
24-25	40.148243296191	41.0	41.0	41.0	37.0	41.0
26-27	40.0772747639551	41.0	41.0	41.0	37.0	41.0
28-29	40.04261927635456	41.0	41.0	41.0	37.0	41.0
30-31	40.00362946880358	41.0	41.0	41.0	37.0	41.0
32-33	39.9778995809785	41.0	41.0	41.0	37.0	41.0
34-35	39.94493033158327	41.0	41.0	41.0	37.0	41.0
36-37	39.815455447069695	41.0	41.0	41.0	37.0	41.0
38-39	39.82260653762243	41.0	41.0	41.0	37.0	41.0
40-41	39.80562224433541	41.0	41.0	41.0	37.0	41.0
42-43	39.77022460705902	41.0	41.0	41.0	37.0	41.0
44-45	39.7196238031993	41.0	41.0	41.0	37.0	41.0
46-47	39.68318437605918	41.0	41.0	41.0	37.0	41.0
48-49	39.63531776249279	41.0	41.0	41.0	37.0	41.0
50-51	39.53523842751353	41.0	41.0	41.0	37.0	41.0
52-53	39.48540089398753	41.0	41.0	41.0	37.0	41.0
54-55	39.400789880086364	41.0	41.0	41.0	37.0	41.0
56-57	39.33275092455314	41.0	41.0	41.0	37.0	41.0
58-59	39.238461088707965	41.0	41.0	41.0	37.0	41.0
60-61	39.15638858602577	41.0	41.0	41.0	37.0	41.0
62-63	39.02035197324339	41.0	41.0	41.0	37.0	41.0
64-65	38.90621432840389	41.0	41.0	41.0	32.0	41.0
66-67	38.745448904706095	41.0	41.0	41.0	32.0	41.0
68-69	38.57577314859061	41.0	39.0	41.0	32.0	41.0
70-71	38.4834075038592	41.0	37.0	41.0	32.0	41.0
72-73	38.32307304564081	41.0	37.0	41.0	32.0	41.0
74-75	38.13010161036318	41.0	37.0	41.0	32.0	41.0
76-77	36.34912436093751	39.0	34.5	41.0	27.0	41.0
78-79	37.12180416724401	41.0	37.0	41.0	29.5	41.0
80-81	38.02298977919499	41.0	37.0	41.0	32.0	41.0
82-83	38.08947287878274	41.0	37.0	41.0	32.0	41.0
84-85	38.091480170498826	41.0	37.0	41.0	32.0	41.0
86-87	38.04817467600277	41.0	37.0	41.0	32.0	41.0
88-89	37.92896256141454	41.0	37.0	41.0	32.0	41.0
90-91	37.83361220514529	41.0	37.0	41.0	32.0	41.0
92-93	37.808236653345745	41.0	37.0	41.0	32.0	41.0
94-95	37.79342695860283	41.0	37.0	41.0	32.0	41.0
96-97	37.7770020782142	41.0	37.0	41.0	32.0	41.0
98-99	37.75817849918329	41.0	37.0	41.0	32.0	41.0
100-101	36.860889396594025	41.0	34.5	41.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	5.0
17	15.0
18	43.0
19	215.0
20	820.0
21	2587.0
22	6676.0
23	15303.0
24	30265.0
25	52521.0
26	85651.0
27	126447.0
28	175303.0
29	230545.0
30	291798.0
31	355293.0
32	423540.0
33	508916.0
34	609924.0
35	749724.0
36	978906.0
37	1428058.0
38	2613922.0
39	6976542.0
40	1.5088863E7
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.54564989998721	10.912805445410537	5.5329642123134	42.008580442288846
2	25.134044637201562	12.547225156911528	30.326903884227185	31.991826321659715
3	21.361260606836336	14.364613521220893	20.652991344260922	43.62113452768185
4	24.70820729904572	21.337347049609935	20.62606377632225	33.32838187502209
5	25.380757984803726	25.986220095855593	23.280467085544	25.35255483379668
6	21.253137572095994	30.497982838969566	24.93134485455736	23.31753473437708
7	16.611847801320003	25.11895613026363	39.852180759142456	18.417015309273907
8	19.101916903204256	24.68145835907045	31.75157116056706	24.465053577158233
9	19.054039715226544	22.716495116738056	34.448131192486656	23.781333975548748
10-11	21.52209671193143	30.804609590898874	25.65598503350185	22.017308663667848
12-13	22.109384326156547	25.130290395550738	27.888757251060042	24.871568027232673
14-15	21.704964409253673	25.833457813645307	27.35491155030905	25.10666622679197
16-17	22.21230810484028	26.248181290231887	26.795165681399087	24.744344923528747
18-19	21.968482385290034	26.58533300220998	26.467153247168635	24.979031365331352
20-21	22.26644625306359	26.312494425138127	26.6600016005524	24.761057721245884
22-23	22.147434028673953	26.458711487683534	26.584201364189635	24.80965311945288
24-25	22.131128669587543	26.09475099587116	26.587160867332326	25.18695946720897
26-27	22.01170738065048	26.22221865243179	26.516735576810046	25.249338390107688
28-29	22.023848091188366	26.38211489253166	26.541810204191897	25.052226812088072
30-31	22.144816302793554	26.28934137138854	26.25382322116665	25.312019104651252
32-33	22.0883516375527	26.275672799912186	26.37320179492763	25.262773767607484
34-35	22.474056488387102	26.38942772259259	26.09051897851839	25.04599681050192
36-37	22.05958412367789	26.17783275255047	26.2126208661759	25.549962257595737
38-39	21.993573531741127	26.240463063676454	26.497730236551693	25.26823316803072
40-41	22.3733827941528	26.59197162007933	26.064052728088228	24.97059285767964
42-43	22.331738189820765	26.419148056722253	26.12745534964477	25.121658403812212
44-45	22.346213726164347	26.444156281421854	26.278828194032865	24.93080179838093
46-47	22.23609038835118	26.19663322730514	26.42135605159528	25.145920332748407
48-49	21.9861333369407	26.070023094195566	26.34989863872726	25.593944930136477
50-51	22.178832105988437	26.01062510546102	26.50801253373655	25.302530254813988
52-53	22.665566528072443	26.430233556754885	25.832627875177593	25.071572039995075
54-55	22.616772768028547	26.280195589974113	25.83554639564673	25.267485246350606
56-57	22.219427994051618	26.12451244042519	26.449263285763674	25.20679627975952
58-59	22.18651293645986	26.15492683813866	26.548504363131194	25.110055862270286
60-61	22.365505227761176	25.876797528138358	26.50193648304398	25.255760761056482
62-63	22.155228673314088	26.07612841138866	26.394713780616296	25.373929134680957
64-65	22.22427810355548	26.401588156406554	26.045714013675198	25.32841972636277
66-67	22.353805131217495	26.19390981683951	25.96326540394291	25.489019648000095
68-69	22.304952838172543	26.381187779623122	26.27779573693097	25.036063645273366
70-71	22.534709500553184	26.736305871090888	26.065254280526496	24.663730347829432
72-73	22.76552804554285	26.089380764242204	25.94033456302533	25.204756627189617
74-75	22.579398705701394	25.972100709119832	26.36412276668746	25.084377818491316
76-77	22.283310261033446	26.28737238626981	26.417393692607376	25.01192366008937
78-79	22.281057955112797	26.271242218696965	26.102567174340503	25.345132651849735
80-81	22.35558225816611	26.378970029249913	26.195625158953682	25.069822553630296
82-83	22.70327316216701	26.5141763839307	25.82099444121845	24.961556012683843
84-85	22.578994593599358	26.322659656320884	25.87127916687248	25.227066583207282
86-87	22.47304213469394	26.157509055247413	26.247384730211277	25.122064079847373
88-89	22.678245777659416	26.1896174351535	26.153395249456608	24.978741537730475
90-91	22.753904208077273	25.966208313162483	26.185614389857037	25.094273088903208
92-93	22.517093685586552	25.895654266121415	26.345933899532547	25.24131814875949
94-95	22.670559643999784	25.994003793383087	26.212956127390708	25.122480435226418
96-97	22.786981857338624	25.89720603450527	26.010291792538364	25.30552031561775
98-99	22.65892791020309	26.177762838132544	26.155507290399093	25.007801961265265
100-101	22.714805789291017	26.386366324299555	25.98598271201799	24.912845174391435
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	138.0
1	101.0
2	59.0
3	61.5
4	73.0
5	82.5
6	103.0
7	148.0
8	191.0
9	232.0
10	267.0
11	328.0
12	403.0
13	447.0
14	509.0
15	561.0
16	643.0
17	810.0
18	1007.5
19	1257.5
20	1604.0
21	2096.5
22	2993.5
23	4263.5
24	6032.0
25	8719.5
26	12938.5
27	18352.5
28	24693.5
29	34318.5
30	48434.5
31	67003.0
32	93796.5
33	129952.0
34	168150.0
35	218195.5
36	293730.0
37	391957.5
38	512913.5
39	680126.0
40	883145.5
41	1083398.0
42	1271590.0
43	1456165.5
44	1641911.5
45	1807321.5
46	1923150.0
47	1922623.0
48	1838928.0
49	1732327.5
50	1616675.5
51	1504206.0
52	1385329.0
53	1225673.0
54	1055425.0
55	933509.0
56	810203.0
57	678984.5
58	571065.5
59	465074.5
60	370787.5
61	333910.5
62	296680.0
63	242242.0
64	197659.0
65	160736.5
66	141463.5
67	118235.0
68	87022.0
69	64603.0
70	49379.5
71	38367.5
72	31994.5
73	29649.5
74	24745.0
75	16498.0
76	9800.5
77	3300.0
78	338.5
79	83.0
80	24.0
81	9.5
82	4.0
83	2.5
84	1.5
85	1.0
86	1.0
87	1.0
88	0.5
89	0.5
90	0.5
91	0.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.5
97	1.5
98	1.5
99	1.5
100	3.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.023783909427595052
2	0.0
3	0.002357579209052011
4	0.0
5	0.0
6	0.0
7	0.0
8	8.194620147325612E-4
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0022811611243448086
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	5.33300676254524E-4
26-27	0.0
28-29	6.422370948796859E-4
30-31	0.0
32-33	8.129583479489696E-6
34-35	7.316625131540726E-5
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	2.9266500526162903E-5
74-75	5.511857599094014E-4
76-77	0.0
78-79	0.0014503176927409616
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0016730682800789793
88-89	3.73960840056526E-5
90-91	0.0
92-93	3.3656475605087335E-4
94-95	0.0014340585257819822
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3.0751883E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.993597878301077
#Duplication Level	Percentage of deduplicated	Percentage of total
1	62.87295090241661	15.714212522771675
2	14.855349199054354	7.425772484458132
3	6.550675237312539	4.911748281382023
4	3.6584127057150724	3.657475841580397
5	2.2429868534825514	2.803015573112935
6	1.563787744173308	2.345080922692993
7	1.1954428052990376	2.091489173350662
8	0.8783877199251722	1.7563255562438005
9	0.708078399108725	1.592768409627119
>10	4.696741697299186	22.369436562488772
>50	0.4392691482788292	7.608448742759124
>100	0.29564443857430506	14.226143328135512
>500	0.026463025717530387	4.563020679356232
>1k	0.014740836303592757	6.751398848846395
>5k	8.391875320097996E-4	1.3898596482009606
>10k+	2.3009980716397728E-4	0.7938034249932067
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.2762833742571147E-5	0.0	0.0	0.0	0.0
2	3.902200070155054E-5	0.0	0.0	0.0	0.0
3	6.828850122771344E-5	0.0	0.0	0.0	6.503666783591756E-6
4	1.1706600210465161E-4	0.0	0.0	0.0	6.503666783591756E-6
5	1.33325169063631E-4	0.0	0.0	0.0	6.503666783591756E-6
6	1.8210266994056918E-4	0.0	2.2762833742571147E-5	0.0	6.503666783591756E-6
7	1.918581701159568E-4	0.0	2.6014667134367025E-5	0.0	6.503666783591756E-6
8	2.3088017081750734E-4	3.251833391795878E-6	2.9266500526162903E-5	3.251833391795878E-6	6.503666783591756E-6
9	2.536430045600785E-4	3.251833391795878E-6	3.251833391795878E-5	3.251833391795878E-6	9.755500175387635E-6
10-11	2.910390885657311E-4	3.251833391795878E-6	3.577016730975466E-5	3.251833391795878E-6	1.6259166958979392E-5
12-13	3.284351725713837E-4	3.251833391795878E-6	3.902200070155054E-5	4.877750087693817E-6	2.2762833742571147E-5
14-15	3.99975507190893E-4	3.251833391795878E-6	3.902200070155054E-5	6.503666783591756E-6	3.251833391795878E-5
16-17	4.666380917227085E-4	9.755500175387635E-6	3.902200070155054E-5	6.503666783591756E-6	3.902200070155053E-5
18-19	5.154155925996466E-4	9.755500175387635E-6	4.064791739744847E-5	6.503666783591756E-6	5.8533001052325805E-5
20-21	5.706967602601765E-4	9.755500175387635E-6	4.2273834093346414E-5	6.503666783591756E-6	6.991441792361138E-5
22-23	6.373593447919922E-4	1.1381416871285574E-5	4.2273834093346414E-5	6.503666783591756E-6	7.154033461950932E-5
24-25	7.511735135048479E-4	1.4633250263081451E-5	4.2273834093346414E-5	6.503666783591756E-6	7.316625131540726E-5
26-27	8.942541827438664E-4	2.6014667134367025E-5	4.2273834093346414E-5	6.503666783591756E-6	7.47921680113052E-5
28-29	0.0010926160196434152	2.6014667134367025E-5	4.3899750789244354E-5	6.503666783591756E-6	7.966991809899902E-5
30-31	0.001359266357770677	2.6014667134367025E-5	4.7151584181040235E-5	6.503666783591756E-6	8.617358488259077E-5
32-33	0.0018015156990549164	2.6014667134367025E-5	4.877750087693817E-5	6.503666783591756E-6	9.755500175387634E-5
34-35	0.002346197792180726	2.6014667134367025E-5	4.877750087693817E-5	8.129583479489696E-6	1.0731050192926397E-4
36-37	0.0031006231390773697	2.6014667134367025E-5	4.877750087693817E-5	9.755500175387635E-6	1.1218825201695779E-4
38-39	0.004233887076118233	2.6014667134367025E-5	4.877750087693817E-5	9.755500175387635E-6	1.3820291915132482E-4
40-41	0.0057541191867828065	2.6014667134367025E-5	4.877750087693817E-5	9.755500175387635E-6	1.6096575289389594E-4
42-43	0.007635304803936722	2.9266500526162903E-5	4.877750087693817E-5	9.755500175387635E-6	1.674694196774877E-4
44-45	0.0097831407592179	4.8777500876938176E-5	4.877750087693817E-5	9.755500175387635E-6	1.707212530692836E-4
46-47	0.012313067138035092	5.8533001052325805E-5	5.5281167660529924E-5	9.755500175387635E-6	1.7234716976518154E-4
48-49	0.015491734278515563	5.8533001052325805E-5	5.5281167660529924E-5	9.755500175387635E-6	1.8047675324467122E-4
50-51	0.02002966777676671	5.8533001052325805E-5	5.5281167660529924E-5	9.755500175387635E-6	1.8535450333236503E-4
52-53	0.025530143958989438	5.8533001052325805E-5	5.5281167660529924E-5	9.755500175387635E-6	1.8535450333236503E-4
54-55	0.03170862740340161	5.8533001052325805E-5	5.5281167660529924E-5	1.1381416871285574E-5	1.8535450333236503E-4
56-57	0.03901224520137515	6.828850122771344E-5	6.178483444412169E-5	1.3007333567183513E-5	1.9348408681185475E-4
58-59	0.047637733273113714	6.991441792361138E-5	6.828850122771344E-5	1.3007333567183513E-5	1.999877535954465E-4
60-61	0.05850698638519143	7.154033461950932E-5	7.47921680113052E-5	1.3007333567183513E-5	2.0486550368314032E-4
62-63	0.07232402646693212	7.154033461950932E-5	7.47921680113052E-5	1.3007333567183513E-5	2.081173370749362E-4
64-65	0.08916852343643478	7.154033461950932E-5	7.47921680113052E-5	1.3007333567183513E-5	2.081173370749362E-4
66-67	0.10873643087156647	7.154033461950932E-5	7.47921680113052E-5	1.3007333567183513E-5	2.081173370749362E-4
68-69	0.1320439467072634	7.154033461950932E-5	7.47921680113052E-5	1.3007333567183513E-5	2.1136917046673208E-4
70-71	0.15964225670343504	7.47921680113052E-5	7.47921680113052E-5	1.3007333567183513E-5	2.1299508716263E-4
72-73	0.19399299873767079	7.641808470720314E-5	7.47921680113052E-5	1.3007333567183513E-5	2.2275058733801765E-4
74-75	0.2372911603494329	7.804400140310108E-5	7.47921680113052E-5	1.3007333567183513E-5	2.2437650403391558E-4
76-77	0.28980339187684867	8.454766818669283E-5	7.47921680113052E-5	1.3007333567183513E-5	2.2437650403391558E-4
78-79	0.3529117225114312	8.454766818669283E-5	7.47921680113052E-5	1.3007333567183513E-5	2.2437650403391558E-4
80-81	0.4271933526802245	8.454766818669283E-5	7.47921680113052E-5	1.3007333567183513E-5	2.260024207298135E-4
82-83	0.5166545411219209	8.454766818669283E-5	7.47921680113052E-5	1.3007333567183513E-5	2.2762833742571146E-4
84-85	0.6269534779382453	8.454766818669283E-5	7.47921680113052E-5	1.3007333567183513E-5	2.3250608751340527E-4
86-87	0.7621012996179779	8.454766818669283E-5	7.47921680113052E-5	1.3007333567183513E-5	2.3413200420930322E-4
88-89	0.9227857689234835	8.617358488259077E-5	7.804400140310108E-5	1.3007333567183513E-5	2.3413200420930322E-4
90	1.0595513777156345	8.779950157848871E-5	7.804400140310108E-5	1.3007333567183513E-5	2.3413200420930322E-4
>>END_MODULE
