##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	SRR19654142_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	29787146
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.652794027329776	32.0	32.0	32.0	32.0	32.0
2	31.687169089646922	32.0	32.0	32.0	32.0	32.0
3	35.81388418346625	37.0	37.0	37.0	32.0	37.0
4	36.392127564017045	37.0	37.0	37.0	37.0	37.0
5	36.568682746578006	37.0	37.0	37.0	37.0	37.0
6	40.17566271706595	41.0	41.0	41.0	37.0	41.0
7	40.198604995591054	41.0	41.0	41.0	37.0	41.0
8	40.23375539234272	41.0	41.0	41.0	41.0	41.0
9	40.29168078069648	41.0	41.0	41.0	41.0	41.0
10-11	40.335803772540004	41.0	41.0	41.0	41.0	41.0
12-13	40.324705478665194	41.0	41.0	41.0	41.0	41.0
14-15	40.32167143505457	41.0	41.0	41.0	41.0	41.0
16-17	40.31771007870307	41.0	41.0	41.0	41.0	41.0
18-19	40.314889096793635	41.0	41.0	41.0	41.0	41.0
20-21	40.28147911518613	41.0	41.0	41.0	41.0	41.0
22-23	40.287564072100096	41.0	41.0	41.0	41.0	41.0
24-25	40.27795408798144	41.0	41.0	41.0	41.0	41.0
26-27	40.199656455841726	41.0	41.0	41.0	41.0	41.0
28-29	40.20976279499889	41.0	41.0	41.0	41.0	41.0
30-31	40.12153940495004	41.0	41.0	41.0	41.0	41.0
32-33	40.14775376600363	41.0	41.0	41.0	41.0	41.0
34-35	40.165106183720994	41.0	41.0	41.0	41.0	41.0
36-37	40.15479873432655	41.0	41.0	41.0	41.0	41.0
38-39	40.08170802936273	41.0	41.0	41.0	37.0	41.0
40-41	40.102718300034525	41.0	41.0	41.0	37.0	41.0
42-43	40.08882813076486	41.0	41.0	41.0	37.0	41.0
44-45	40.082170124657125	41.0	41.0	41.0	37.0	41.0
46-47	40.07241578296893	41.0	41.0	41.0	37.0	41.0
48-49	39.983039194154415	41.0	41.0	41.0	37.0	41.0
50-51	39.94208031880597	41.0	41.0	41.0	37.0	41.0
52-53	39.98536962889966	41.0	41.0	41.0	37.0	41.0
54-55	39.957271116205625	41.0	41.0	41.0	37.0	41.0
56-57	39.94759293152825	41.0	41.0	41.0	37.0	41.0
58-59	39.9471639713318	41.0	41.0	41.0	37.0	41.0
60-61	39.92943884251281	41.0	41.0	41.0	37.0	41.0
62-63	39.88671677373858	41.0	41.0	41.0	37.0	41.0
64-65	39.83267739044217	41.0	41.0	41.0	37.0	41.0
66-67	39.809023496242304	41.0	41.0	41.0	37.0	41.0
68-69	39.78976500467685	41.0	41.0	41.0	37.0	41.0
70-71	39.769390175883245	41.0	41.0	41.0	37.0	41.0
72-73	39.74915990608835	41.0	41.0	41.0	37.0	41.0
74-75	39.72899463077127	41.0	41.0	41.0	37.0	41.0
76-77	39.198702252307086	41.0	39.0	41.0	34.5	41.0
78-79	39.525653817253925	41.0	41.0	41.0	37.0	41.0
80-81	39.70409942933103	41.0	41.0	41.0	37.0	41.0
82-83	39.775870722895036	41.0	41.0	41.0	37.0	41.0
84-85	39.815998048285664	41.0	41.0	41.0	37.0	41.0
86-87	39.819482991086154	41.0	41.0	41.0	37.0	41.0
88-89	39.702247237785045	41.0	41.0	41.0	37.0	41.0
90-91	39.72565530447261	41.0	41.0	41.0	37.0	41.0
92-93	39.6751283422722	41.0	41.0	41.0	37.0	41.0
94-95	39.6668415127787	41.0	41.0	41.0	37.0	41.0
96-97	39.63355077723794	41.0	41.0	41.0	37.0	41.0
98-99	39.58643051939249	41.0	41.0	41.0	37.0	41.0
100-101	39.06579485661366	41.0	39.0	41.0	34.5	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	1.0
14	4.0
15	4.0
16	8.0
17	27.0
18	46.0
19	127.0
20	416.0
21	1228.0
22	3087.0
23	7076.0
24	13115.0
25	22865.0
26	38119.0
27	58651.0
28	86262.0
29	119416.0
30	156815.0
31	200155.0
32	247502.0
33	301781.0
34	363950.0
35	446140.0
36	568485.0
37	771085.0
38	1209958.0
39	2870563.0
40	2.2300259E7
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.63933176637137	6.481766633384513	5.595835874507066	52.283065725737046
2	19.0490421606689	7.720951178068554	38.570288002751255	34.65971865851129
3	18.787625516023983	9.50337852253186	22.738717418692158	48.970278542752
4	23.949743288598377	16.46986589450362	21.629168501070897	37.9512223158271
5	26.242759208955434	22.318919039776418	25.589594921245563	25.84872683002259
6	22.132382202712538	28.004663488069653	25.78141927393783	24.081535035279984
7	16.27286145507193	23.924000641081893	41.38182288427364	18.42131501957254
8	18.44193800909963	23.160258455106778	34.586267512839264	23.81153602295433
9	18.98215760583441	20.89972970220108	36.74078409526042	23.377328596704096
10-11	21.32253725698979	30.49861576333255	26.9039882489655	21.274858730712157
12-13	21.795664814615	24.75959261085302	29.29125536229621	24.153487212235774
14-15	21.383737132788756	25.370700167112354	28.680016877078458	24.56554582302044
16-17	22.28498916900317	25.557456252669812	27.324323460525985	24.833231117801038
18-19	22.01573537029929	25.562687381940364	26.99794405794406	25.42363318981628
20-21	22.24088873771257	25.45813385411278	27.332695787639405	24.968281620535247
22-23	22.145109168901243	25.81934167173988	27.250737616823038	24.78481154253583
24-25	22.124412203802247	25.59135646257727	26.802467577418554	25.481763756201925
26-27	21.870047570183463	25.70830048639101	27.26189847123991	25.15975347218562
28-29	22.068090999702235	25.8446732809136	26.95357744970136	25.1336582696828
30-31	22.04885644100355	25.53818641249362	26.996864080201338	25.4160930663015
32-33	22.107171663911675	25.422106569054986	27.094821370264878	25.375900396768458
34-35	22.230016598434773	25.584233548256012	26.72548924291035	25.460260610398862
36-37	22.090330877794248	25.488759908195966	26.613773716585232	25.80713549742455
38-39	22.234934619820955	25.687764527885133	27.095746399832326	24.981554452461584
40-41	22.39500722895708	25.81854770510743	26.788387178818674	24.99805788711681
42-43	22.40189274930871	25.39942228772102	26.63633333653382	25.562351626436453
44-45	22.12854497708508	25.490508556946008	27.09155821776279	25.289388248206123
46-47	22.182139199626597	25.691615528163847	26.88254896062931	25.243696311580248
48-49	22.25279991577575	25.411964610506825	26.885915824228345	25.449319649489077
50-51	22.133735709630116	25.473318865696204	27.03600899347348	25.3569364312002
52-53	22.75933690126601	25.71796008842382	26.64153590239392	24.881167107916248
54-55	22.67761234997136	25.420352456727475	26.480302946781137	25.421732246520023
56-57	22.416340561956044	25.38547745529956	27.046187599699678	25.151994383044713
58-59	22.466006683093237	25.533132541749186	26.83869748335731	25.162163291800262
60-61	22.181600062269123	25.221265119662178	26.96806704989078	25.629067768177915
62-63	22.319589346434267	25.29423421316817	27.201137171763644	25.18503926863392
64-65	22.557400099518052	25.571113786553802	26.660001019587508	25.211485094340635
66-67	22.45317925999098	25.286012926575356	26.775968914876643	25.484838898557022
68-69	22.443675955724967	25.31693613206108	26.991008453694842	25.24837945851911
70-71	22.526692840052515	25.643830998241558	26.805465165721404	25.024010995984526
72-73	22.505669056041825	25.48156342336389	26.51569237281074	25.497075147783544
74-75	22.35729633178016	25.417923556691196	26.889185691035994	25.33559442049265
76-77	22.336329443115297	25.533406555806103	26.762165301948027	25.36809869913057
78-79	22.314471561473916	25.37618324951921	26.793986130594256	25.515359058412614
80-81	22.371380038593852	25.403037653597966	27.00372842037843	25.221853887429752
82-83	22.30702474268371	25.775015665504014	26.576572463258458	25.341387128553823
84-85	22.64259981221424	25.30938523260511	26.50742589015607	25.540589065024577
86-87	22.442548005703863	25.434978383899594	26.90755896272933	25.214914647667214
88-89	22.641472600295444	25.598018017570396	26.76357278404584	24.996936598088315
90-91	22.584633325615076	25.35589669193682	26.620216800732333	25.43925318171577
92-93	22.463260231144027	25.5012355138834	26.79249221702126	25.243012037951313
94-95	22.788703892611935	25.690960792282684	26.46729397975892	25.05304133534646
96-97	22.80903313705627	25.626741517512276	26.282368480098935	25.281856865332514
98-99	22.620851610802273	25.89939415252484	26.63479571269644	24.844958523976445
100-101	22.725883171217546	25.90350549193266	26.54723953748372	24.823371799366075
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	35.0
1	29.0
2	18.0
3	19.0
4	26.0
5	37.5
6	55.0
7	76.5
8	89.5
9	92.5
10	96.5
11	105.5
12	135.5
13	167.5
14	190.5
15	224.5
16	287.5
17	391.0
18	510.0
19	653.0
20	903.5
21	1237.0
22	1712.5
23	2431.5
24	4256.0
25	5953.5
26	7595.0
27	10982.0
28	16340.5
29	23380.5
30	32639.0
31	44684.5
32	63009.5
33	87358.0
34	120191.5
35	170763.5
36	237513.0
37	319898.0
38	435774.0
39	596278.0
40	795956.5
41	1018858.5
42	1247229.5
43	1470454.0
44	1663721.0
45	1799055.5
46	1897708.0
47	1936928.0
48	1872912.0
49	1765686.5
50	1646504.0
51	1506253.0
52	1376605.0
53	1252650.0
54	1090018.0
55	916445.5
56	780731.0
57	646456.0
58	509414.5
59	415701.5
60	343749.5
61	289137.5
62	246453.0
63	197837.5
64	171231.5
65	149803.5
66	133687.5
67	114907.5
68	75982.0
69	50982.5
70	47199.0
71	44139.0
72	35454.0
73	35365.5
74	29337.0
75	15784.5
76	7821.5
77	2215.0
78	437.0
79	118.0
80	43.0
81	19.0
82	9.5
83	4.5
84	2.5
85	2.0
86	0.5
87	1.0
88	1.0
89	0.5
90	0.0
91	0.0
92	1.0
93	1.0
94	0.5
95	0.5
96	1.0
97	1.5
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05854203017637205
2	0.0
3	4.3642986139054746E-5
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	5.086086461589842E-4
12-13	0.0
14-15	0.0
16-17	0.0013898612508898972
18-19	0.001871612674809463
20-21	0.0
22-23	0.0
24-25	1.2085750007738237E-4
26-27	0.0
28-29	2.7360795156407394E-4
30-31	0.0017138264941528806
32-33	0.0
34-35	0.0
36-37	1.6785763899636439E-6
38-39	0.0027696510434400126
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.002136827744423719
48-49	0.0
50-51	0.0014586828828784067
52-53	5.002157642091659E-4
54-55	0.0
56-57	6.378590281861848E-4
58-59	1.3764326397701883E-4
60-61	0.0010457530909473503
62-63	0.003251402467359579
64-65	0.0019823987165470637
66-67	9.836457645186955E-4
68-69	3.743225349618926E-4
70-71	9.14824132530186E-4
72-73	0.0
74-75	0.0
76-77	0.0010004315284183319
78-79	3.3571527799272878E-6
80-81	0.0018027910428209536
82-83	0.0012119321535537509
84-85	3.357152779927288E-5
86-87	0.0010776460423566594
88-89	0.0
90-91	0.0020915061818947006
92-93	0.0011750034729745508
94-95	0.0
96-97	2.8703656268378315E-4
98-99	0.0012035392716039327
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2.9787146E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.246169661777024
#Duplication Level	Percentage of deduplicated	Percentage of total
1	52.43532728774347	11.664851871139478
2	18.220937243063954	8.106921226115848
3	8.649454529300888	5.772516988219599
4	4.830911808094933	4.298771348158475
5	3.036578166677004	3.377611654357223
6	2.0605002073184697	2.750294232008005
7	1.5570834281892927	2.4247399484728285
8	1.1987713895937546	2.1334457374869493
9	0.9368704102865228	1.8757600288499372
>10	6.130261859319202	26.001620816416533
>50	0.5729592630894973	8.821832252066807
>100	0.3350485954566998	13.961053307692836
>500	0.02470877028513222	3.7343947161630746
>1k	0.009913183381287112	3.8095377127281385
>5k	5.29460014200613E-4	0.8117034621026818
>10k+	1.4439818569107624E-4	0.4549446980216701
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.0071458339781865E-5	0.0	6.714305559854576E-6	0.0	0.0
2	1.3428611119709153E-5	0.0	6.714305559854576E-6	0.0	0.0
3	2.3500069459491017E-5	3.357152779927288E-6	6.714305559854576E-6	0.0	0.0
4	4.028583335912746E-5	3.357152779927288E-6	2.6857222239418306E-5	0.0	0.0
5	4.3642986139054746E-5	3.357152779927288E-6	3.0214375019345594E-5	0.0	0.0
6	9.400027783796407E-5	3.357152779927288E-6	8.057166671825492E-5	0.0	0.0
7	1.0071458339781865E-4	3.357152779927288E-6	8.728597227810949E-5	0.0	0.0
8	1.2421465285730965E-4	3.357152779927288E-6	8.728597227810949E-5	0.0	0.0
9	1.2421465285730965E-4	3.357152779927288E-6	8.728597227810949E-5	0.0	0.0
10-11	1.4267899314690974E-4	1.678576389963644E-5	8.728597227810949E-5	0.0	3.357152779927288E-6
12-13	1.7289336816625532E-4	5.371444447883661E-5	8.728597227810949E-5	0.0	8.39288194981822E-6
14-15	2.585007640544012E-4	6.378590281861848E-5	8.728597227810949E-5	0.0	1.0071458339781865E-5
16-17	3.189295140930924E-4	7.050020837847305E-5	8.728597227810949E-5	0.0	1.3428611119709153E-5
18-19	3.760011113518563E-4	7.385736115840034E-5	8.728597227810949E-5	0.0	1.3428611119709153E-5
20-21	4.5153704890022025E-4	7.385736115840034E-5	8.728597227810949E-5	0.0	1.3428611119709153E-5
22-23	5.086086461589842E-4	7.553593754836398E-5	8.728597227810949E-5	0.0	1.3428611119709153E-5
24-25	6.596805212557121E-4	8.225024310821855E-5	9.400027783796407E-5	0.0	1.3428611119709153E-5
26-27	7.906094796728763E-4	1.3428611119709153E-4	9.903600700785501E-5	0.0	1.3428611119709153E-5
28-29	9.66860000619059E-4	1.376432639770188E-4	1.22536076467346E-4	0.0	1.3428611119709153E-5
30-31	0.0012488608341329512	1.376432639770188E-4	1.3260753480712786E-4	0.0	1.3428611119709153E-5
32-33	0.0015107187509672796	1.410004167569461E-4	1.410004167569461E-4	0.0	1.3428611119709153E-5
34-35	0.0018413982997901174	1.410004167569461E-4	1.410004167569461E-4	0.0	1.8464340289600085E-5
36-37	0.0022560066681111377	1.410004167569461E-4	1.410004167569461E-4	0.0	2.014291667956373E-5
38-39	0.00284686555737834	1.410004167569461E-4	1.410004167569461E-4	0.0	2.014291667956373E-5
40-41	0.0036945466343099803	1.410004167569461E-4	1.410004167569461E-4	0.0	2.014291667956373E-5
42-43	0.004668120940488895	1.5275045148669162E-4	1.443575695368734E-4	0.0	2.014291667956373E-5
44-45	0.005745766982845554	1.8128625011607356E-4	1.4771472231680068E-4	0.0	2.014291667956373E-5
46-47	0.007009735004488177	2.1989350708523738E-4	1.4771472231680068E-4	0.0	2.014291667956373E-5
48-49	0.008809168894529203	2.2157208347520102E-4	1.4771472231680068E-4	0.0	2.014291667956373E-5
50-51	0.011194425944667543	2.2157208347520102E-4	1.4771472231680068E-4	0.0	2.014291667956373E-5
52-53	0.014004362821466682	2.3500069459491017E-4	1.4771472231680068E-4	0.0	2.014291667956373E-5
54-55	0.0172540867124363	2.4171500015476475E-4	1.4771472231680068E-4	0.0	2.014291667956373E-5
56-57	0.020962061957865985	2.585007640544012E-4	1.7792909733614626E-4	0.0	2.517864584945466E-5
58-59	0.02585007640544012	2.585007640544012E-4	2.2325065986516466E-4	0.0	3.0214375019345594E-5
60-61	0.03234280988181949	2.585007640544012E-4	2.249292362551283E-4	0.0	3.0214375019345594E-5
62-63	0.04104622846378099	2.585007640544012E-4	2.249292362551283E-4	0.0	3.0214375019345594E-5
64-65	0.05220708288064926	2.585007640544012E-4	2.249292362551283E-4	0.0	3.189295140930924E-5
66-67	0.06566758695176772	2.585007640544012E-4	2.249292362551283E-4	0.0	3.357152779927288E-5
68-69	0.0824281722055547	2.585007640544012E-4	2.249292362551283E-4	0.0	3.357152779927288E-5
70-71	0.10434198697653008	2.6185791683432845E-4	2.249292362551283E-4	0.0	3.357152779927288E-5
72-73	0.1332621795992137	2.6185791683432845E-4	2.3164354181498287E-4	0.0	4.028583335912746E-5
74-75	0.17180565066555892	2.9710802102356497E-4	2.3164354181498287E-4	0.0	4.028583335912746E-5
76-77	0.21886957548735955	5.740731253675662E-4	2.3164354181498287E-4	0.0	4.028583335912746E-5
78-79	0.27840196573381015	6.193946878965846E-4	2.3164354181498287E-4	0.0	4.028583335912746E-5
80-81	0.3541544396364794	6.277875698464029E-4	2.3164354181498287E-4	0.0	4.19644097490911E-5
82-83	0.44977790084353836	6.277875698464029E-4	2.3164354181498287E-4	0.0	4.867871530894568E-5
84-85	0.573393301929631	6.361804517962212E-4	2.3164354181498287E-4	0.0	5.035729169890932E-5
86-87	0.7320405922742649	6.613590976456758E-4	2.3164354181498287E-4	0.0	5.035729169890932E-5
88-89	0.9256459145162816	7.352164588040761E-4	2.3164354181498287E-4	0.0	5.035729169890932E-5
90	1.0933642316722791	7.68787986603349E-4	2.3164354181498287E-4	0.0	5.035729169890932E-5
>>END_MODULE
