##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	SRR19654120_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	26838591
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.641667291699477	32.0	32.0	32.0	32.0	32.0
2	31.614245956503453	32.0	32.0	32.0	32.0	32.0
3	35.70109667828687	37.0	37.0	37.0	32.0	37.0
4	36.33316395037281	37.0	37.0	37.0	37.0	37.0
5	36.514132951316256	37.0	37.0	37.0	37.0	37.0
6	40.09847167461213	41.0	41.0	41.0	37.0	41.0
7	40.14517822489265	41.0	41.0	41.0	37.0	41.0
8	40.23223946443388	41.0	41.0	41.0	37.0	41.0
9	40.24329514913804	41.0	41.0	41.0	41.0	41.0
10-11	40.29667261593576	41.0	41.0	41.0	41.0	41.0
12-13	40.262123186720196	41.0	41.0	41.0	41.0	41.0
14-15	40.210462538812116	41.0	41.0	41.0	41.0	41.0
16-17	40.23629439041714	41.0	41.0	41.0	41.0	41.0
18-19	40.2319803040331	41.0	41.0	41.0	41.0	41.0
20-21	40.21014855064485	41.0	41.0	41.0	41.0	41.0
22-23	40.20081410011427	41.0	41.0	41.0	41.0	41.0
24-25	40.18398959542995	41.0	41.0	41.0	41.0	41.0
26-27	40.134227780437506	41.0	41.0	41.0	41.0	41.0
28-29	40.11311631448909	41.0	41.0	41.0	37.0	41.0
30-31	40.087196026795894	41.0	41.0	41.0	37.0	41.0
32-33	40.07959437960063	41.0	41.0	41.0	37.0	41.0
34-35	40.07085915948419	41.0	41.0	41.0	37.0	41.0
36-37	39.97213687559082	41.0	41.0	41.0	37.0	41.0
38-39	40.0124492004815	41.0	41.0	41.0	37.0	41.0
40-41	40.01154641463853	41.0	41.0	41.0	37.0	41.0
42-43	40.00039761774379	41.0	41.0	41.0	37.0	41.0
44-45	39.98172743122022	41.0	41.0	41.0	37.0	41.0
46-47	39.96178078051862	41.0	41.0	41.0	37.0	41.0
48-49	39.93887529341611	41.0	41.0	41.0	37.0	41.0
50-51	39.87963306270437	41.0	41.0	41.0	37.0	41.0
52-53	39.873171173553786	41.0	41.0	41.0	37.0	41.0
54-55	39.83237169566763	41.0	41.0	41.0	37.0	41.0
56-57	39.820390328985596	41.0	41.0	41.0	37.0	41.0
58-59	39.785660543804255	41.0	41.0	41.0	37.0	41.0
60-61	39.78692234625879	41.0	41.0	41.0	37.0	41.0
62-63	39.734121791266915	41.0	41.0	41.0	37.0	41.0
64-65	39.73899915237726	41.0	41.0	41.0	37.0	41.0
66-67	39.680114708704345	41.0	41.0	41.0	37.0	41.0
68-69	39.64766894804575	41.0	41.0	41.0	37.0	41.0
70-71	39.64771112611687	41.0	41.0	41.0	37.0	41.0
72-73	39.623370653101716	41.0	41.0	41.0	37.0	41.0
74-75	39.577466697115355	41.0	41.0	41.0	37.0	41.0
76-77	39.01736715612232	41.0	39.0	41.0	34.5	41.0
78-79	39.380167386581505	41.0	41.0	41.0	37.0	41.0
80-81	39.66421583010822	41.0	41.0	41.0	37.0	41.0
82-83	39.68867817986421	41.0	41.0	41.0	37.0	41.0
84-85	39.70283205254702	41.0	41.0	41.0	37.0	41.0
86-87	39.68094494975537	41.0	41.0	41.0	37.0	41.0
88-89	39.63625588616034	41.0	41.0	41.0	37.0	41.0
90-91	39.60239177608094	41.0	41.0	41.0	37.0	41.0
92-93	39.55671080124884	41.0	41.0	41.0	37.0	41.0
94-95	39.53489307616782	41.0	41.0	41.0	37.0	41.0
96-97	39.51180531422085	41.0	41.0	41.0	37.0	41.0
98-99	39.46533532628445	41.0	41.0	41.0	37.0	41.0
100-101	38.930824069713644	41.0	39.0	41.0	34.5	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	3.0
16	4.0
17	24.0
18	56.0
19	149.0
20	480.0
21	1404.0
22	3416.0
23	7271.0
24	13569.0
25	23922.0
26	39613.0
27	61956.0
28	90371.0
29	125120.0
30	164762.0
31	208481.0
32	260090.0
33	314135.0
34	376649.0
35	458328.0
36	571319.0
37	753838.0
38	1130832.0
39	2522210.0
40	1.9710588E7
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.634102726154598	13.056081893388061	8.400164220882154	50.90965115957518
2	22.218908585774862	16.07044870574614	35.13226160046926	26.57838110800973
3	19.427750842463436	19.095475214956725	22.712616756266122	38.76415718631371
4	22.25054959107205	26.615808557163078	20.930059256836543	30.203582594928324
5	22.95230029027977	30.30494410082854	24.5303935664879	22.21236204240379
6	18.958357389178886	31.548712821772202	26.030666065889974	23.462263723158937
7	15.592267865328699	22.936610196861675	40.65618049770198	20.814941440107642
8	18.554957875471047	23.4396490312519	31.085864616152058	26.919528477125
9	17.97542575912424	22.139984919476586	34.029327396509004	25.855261924890172
10-11	21.584232942780044	30.004188744483645	23.60514752805019	24.806430784686125
12-13	21.812912980416893	23.8762198805444	26.640772609858693	27.670094529180016
14-15	20.615505225296154	26.158169107721612	27.459497888298294	25.766827778683936
16-17	21.550920836343458	26.460921514098857	26.375065665705026	25.613091983852655
18-19	21.280055275629035	26.5366501542499	26.49207814225419	25.691216427866877
20-21	21.554602102621555	26.46161268302051	26.71437185357458	25.26941336078336
22-23	21.40221705379392	26.816363049759207	26.543034617577355	25.23838527886952
24-25	21.17430225336198	26.538723519269812	26.562158197697922	25.72481602967029
26-27	21.194005676378467	26.4383476762994	26.956549246568123	25.411097400754013
28-29	21.358571503304443	26.693527064732187	26.618764772678666	25.329136659284707
30-31	21.377191522461068	26.269762075065717	26.700200096197303	25.65284630627592
32-33	21.563437747300185	26.379078749563085	26.58644622386417	25.47103727927256
34-35	21.55539079266043	26.54754443484259	26.366499001232068	25.53056577126491
36-37	21.404704144118444	26.425211740810088	26.410889081323237	25.759195033748234
38-39	21.770490857735417	26.51439861354868	26.49402682875565	25.221083699960257
40-41	21.93102462048026	26.511220354302505	26.286396703910427	25.27135832130681
42-43	21.791516551669947	26.038485030752916	26.332263493266094	25.83773492431104
44-45	21.66805627016709	26.23762551469263	26.537367405017648	25.55695081012263
46-47	21.58638655807229	26.394124043248024	26.529915448989104	25.48957394969058
48-49	21.611825672964724	26.093910816704202	26.351131845930365	25.943131664400713
50-51	21.834201355801426	26.227403294010482	26.343944434340834	25.59445091584726
52-53	22.28318915847706	26.311159553793267	26.01348744425518	25.392163843474496
54-55	21.89142865212261	26.167472800639946	26.09963019295611	25.84146835428134
56-57	21.710640472892187	26.09494104962515	26.595913697555883	25.598504779926785
58-59	21.721656326891377	26.095714190063106	26.58042480695056	25.60220467609496
60-61	21.801504035737196	25.830135792150937	26.435657520173095	25.932702651938772
62-63	22.069576975929923	25.91639777214832	26.460833953615527	25.55319129830623
64-65	21.966186675000934	26.28026188110993	26.121535217702004	25.632016226187133
66-67	21.975544468783774	26.025237315923178	26.247748996957405	25.751469218335643
68-69	21.928286026639775	26.03251042500704	26.50304928451721	25.536154263835982
70-71	22.123350290631873	26.266382613006773	26.297651020502528	25.312616075858823
72-73	22.121909952016836	26.011856812096617	26.16809458622625	25.6981386496603
74-75	22.04486455854727	25.82164756848791	26.44701963300899	25.68646823995583
76-77	21.94772817991824	26.05090557846349	26.255348129117507	25.746018112500764
78-79	21.92738384573504	25.900118517688302	26.392204645112404	25.780292991464254
80-81	22.034034871651794	25.96354070897388	26.531359265469632	25.471065153904686
82-83	22.152446452945313	26.13753456729528	25.972624643372672	25.73739433638674
84-85	22.269220466901558	25.909942515238598	26.02418472713415	25.796652290725696
86-87	22.27882779844467	25.773448459504415	26.407845786980317	25.5398779550706
88-89	22.3969863685804	25.991930125672802	26.285065933433742	25.326017572313052
90-91	22.28334751254267	25.767939904147724	26.29854711821496	25.65016546509465
92-93	22.19277467161481	25.74141514186184	26.32421433404439	25.741595852478955
94-95	22.43454649807186	25.85038929688021	26.04062005887244	25.674444146175485
96-97	22.34012396552412	25.915060146041196	26.014232639858033	25.73058324857665
98-99	22.382665319502056	25.92245434941052	26.323477637108443	25.37140269397898
100-101	22.40171810807803	26.19578278159237	25.976590946968862	25.425908163360738
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	43.0
1	42.0
2	36.5
3	36.5
4	46.0
5	62.0
6	85.0
7	104.0
8	120.5
9	147.5
10	187.5
11	228.0
12	266.0
13	315.5
14	388.5
15	489.5
16	632.5
17	755.5
18	887.0
19	1110.0
20	1454.0
21	1999.5
22	2863.5
23	4142.5
24	6576.5
25	9522.5
26	12914.5
27	18850.0
28	28074.0
29	40238.5
30	56930.0
31	77944.5
32	108545.0
33	148257.5
34	192393.5
35	250751.5
36	325121.0
37	407514.0
38	509667.0
39	630048.5
40	766207.5
41	938797.5
42	1120041.5
43	1276365.0
44	1412455.5
45	1516904.5
46	1583787.0
47	1598015.0
48	1557517.5
49	1481307.0
50	1382796.5
51	1274109.0
52	1164938.0
53	1062211.0
54	946885.0
55	816380.5
56	711240.0
57	620292.5
58	500230.5
59	389729.0
60	319521.5
61	272044.0
62	229106.0
63	189208.0
64	164292.5
65	148466.0
66	126530.5
67	105318.5
68	79741.5
69	55943.0
70	47075.0
71	38117.5
72	27223.5
73	22395.0
74	20253.0
75	17068.5
76	11590.0
77	3863.0
78	575.0
79	161.5
80	54.0
81	26.0
82	14.0
83	6.0
84	2.5
85	1.5
86	1.5
87	1.5
88	1.5
89	1.0
90	0.5
91	1.0
92	1.5
93	1.0
94	0.5
95	0.0
96	0.5
97	0.5
98	0.0
99	1.0
100	1.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.02398412047785966
2	0.0
3	0.00235109212700473
4	0.0
5	0.0
6	0.0
7	0.0
8	7.079358227114084E-4
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0023790369621117594
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	5.961564822832912E-4
26-27	0.0
28-29	7.265657127827612E-4
30-31	0.0
32-33	1.4903912057082281E-5
34-35	8.942347234249369E-5
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	4.0985758156976274E-5
74-75	6.259643063974558E-4
76-77	0.0
78-79	0.0015015691397510399
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0016468822823075921
88-89	2.6081846099893994E-5
90-91	0.0
92-93	3.781867684484629E-4
94-95	0.0014810762606725517
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2.6838591E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.6200710797843
#Duplication Level	Percentage of deduplicated	Percentage of total
1	61.01518939427879	18.68289436199318
2	16.82408717002963	10.30309489997589
3	7.214499089246572	6.627254247533072
4	3.8936930824150258	4.769006357856501
5	2.394916021150234	3.666624939886719
6	1.5595989954609017	2.8653019258183803
7	1.1401240738485954	2.4437476126712063
8	0.824378193001981	2.019401509307584
9	0.6677956049438818	1.8403154001134296
>10	3.9550706966786073	22.379343077613566
>50	0.3055510196046072	6.4503312108543955
>100	0.18411451416573832	10.49761450049075
>500	0.014399634486758541	2.988109533143069
>1k	0.0061589215230847955	3.3048738481033872
>5k	2.9522881241065055E-4	0.5932393282612374
>10k+	1.2836035322202197E-4	0.5688472463775964
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTCCAATCTCCCTCTCATTTACAATTGCATGGGTCACAGGTGCAGTTCGA	27094	0.10095164831864682	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.862989007135285E-5	0.0	0.0	0.0	0.0
2	1.862989007135285E-5	0.0	7.451956028541141E-6	0.0	0.0
3	2.9807824114164563E-5	0.0	1.4903912057082281E-5	0.0	0.0
4	5.5889670214058554E-5	0.0	1.862989007135285E-5	0.0	7.451956028541141E-6
5	7.824553829968198E-5	0.0	2.2355868085623423E-5	0.0	7.451956028541141E-6
6	9.687542837103483E-5	0.0	3.72597801427057E-5	0.0	7.451956028541141E-6
7	9.687542837103483E-5	0.0	4.0985758156976274E-5	0.0	7.451956028541141E-6
8	1.0805336241384654E-4	0.0	4.4711736171246846E-5	0.0	7.451956028541141E-6
9	1.1550531844238768E-4	3.7259780142705703E-6	4.4711736171246846E-5	0.0	7.451956028541141E-6
10-11	1.4158716454228167E-4	7.451956028541141E-6	5.0300703192652696E-5	0.0	9.314945035676426E-6
12-13	1.881618897206638E-4	1.1177934042811711E-5	5.5889670214058554E-5	0.0	1.1177934042811711E-5
14-15	2.6454443901321046E-4	1.1177934042811711E-5	5.5889670214058554E-5	0.0	1.1177934042811711E-5
16-17	3.260230762486749E-4	1.4903912057082281E-5	5.5889670214058554E-5	0.0	1.1177934042811711E-5
18-19	3.8377573546986875E-4	1.4903912057082281E-5	5.5889670214058554E-5	1.8629890071352852E-6	1.1177934042811711E-5
20-21	4.881031198694447E-4	1.4903912057082281E-5	5.9615648228329125E-5	3.7259780142705703E-6	1.1177934042811711E-5
22-23	6.129233833475088E-4	1.4903912057082281E-5	5.9615648228329125E-5	3.7259780142705703E-6	1.1177934042811711E-5
24-25	7.45195602854114E-4	1.6766901064217565E-5	5.9615648228329125E-5	3.7259780142705703E-6	1.1177934042811711E-5
26-27	9.203165695248308E-4	2.2355868085623423E-5	5.9615648228329125E-5	3.7259780142705703E-6	1.1177934042811711E-5
28-29	0.0011326973163382533	2.2355868085623423E-5	7.079358227114083E-5	3.7259780142705703E-6	1.4903912057082281E-5
30-31	0.001423323601451358	2.2355868085623423E-5	7.265657127827611E-5	3.7259780142705703E-6	1.4903912057082281E-5
32-33	0.0017921954248641442	2.2355868085623423E-5	7.45195602854114E-5	3.7259780142705703E-6	1.4903912057082281E-5
34-35	0.002202053006433907	2.2355868085623423E-5	7.45195602854114E-5	3.7259780142705703E-6	1.4903912057082281E-5
36-37	0.002690156126303352	2.421885709275871E-5	7.824553829968198E-5	3.7259780142705703E-6	1.4903912057082281E-5
38-39	0.003344065267807837	2.608184609989399E-5	7.824553829968198E-5	3.7259780142705703E-6	1.4903912057082281E-5
40-41	0.0043351754196038084	2.7944835107029277E-5	7.824553829968198E-5	3.7259780142705703E-6	1.4903912057082281E-5
42-43	0.0055517072412631496	5.0300703192652696E-5	7.824553829968198E-5	3.7259780142705703E-6	1.4903912057082281E-5
44-45	0.00695826394165029	6.334162624259969E-5	7.824553829968198E-5	3.7259780142705703E-6	2.6081846099893994E-5
46-47	0.008519448729629658	7.079358227114083E-5	7.824553829968198E-5	3.7259780142705703E-6	2.9807824114164563E-5
48-49	0.010430875450950462	7.079358227114083E-5	8.942347234249369E-5	3.7259780142705703E-6	2.9807824114164563E-5
50-51	0.013080045819096837	8.197151631395255E-5	9.501243936389954E-5	3.7259780142705703E-6	3.72597801427057E-5
52-53	0.016431563042933216	9.314945035676426E-5	9.687542837103483E-5	3.7259780142705703E-6	3.72597801427057E-5
54-55	0.02048729011146673	9.314945035676426E-5	1.2295727447092882E-4	3.7259780142705703E-6	4.284874716411156E-5
56-57	0.0245933178831929	9.687542837103483E-5	1.658060216350404E-4	3.7259780142705703E-6	4.843771418551742E-5
58-59	0.0289545751488966	9.687542837103483E-5	1.7139498865644624E-4	3.7259780142705703E-6	4.843771418551742E-5
60-61	0.034567761027395214	9.687542837103483E-5	1.7139498865644624E-4	3.7259780142705703E-6	4.843771418551742E-5
62-63	0.04172163881479471	9.873841737817011E-5	1.732579776635815E-4	3.7259780142705703E-6	4.843771418551742E-5
64-65	0.050291388247617023	1.0432738439957596E-4	1.769839556778521E-4	3.7259780142705703E-6	4.843771418551742E-5
66-67	0.06003482075493456	1.0432738439957596E-4	1.7884694468498738E-4	3.7259780142705703E-6	4.843771418551742E-5
68-69	0.07132453413817438	1.0432738439957596E-4	1.8070993369212268E-4	3.7259780142705703E-6	4.843771418551742E-5
70-71	0.08509574887891841	1.0432738439957596E-4	1.8257292269925794E-4	3.7259780142705703E-6	4.843771418551742E-5
72-73	0.1019539364044856	1.0619037340671124E-4	1.8629890071352853E-4	3.7259780142705703E-6	4.843771418551742E-5
74-75	0.12309327266844969	1.266832524851994E-4	1.8629890071352853E-4	3.7259780142705703E-6	4.843771418551742E-5
76-77	0.14893665617543037	1.4158716454228167E-4	1.8629890071352853E-4	3.7259780142705703E-6	4.843771418551742E-5
78-79	0.1814514033169625	1.4158716454228167E-4	1.881618897206638E-4	3.7259780142705703E-6	4.843771418551742E-5
80-81	0.22058721338985343	1.4158716454228167E-4	1.9002487872779908E-4	3.7259780142705703E-6	5.775265922119384E-5
82-83	0.26743579795228445	1.4531314255655225E-4	1.9002487872779908E-4	3.7259780142705703E-6	5.9615648228329125E-5
84-85	0.32373905172592704	1.4717613156368752E-4	1.9561384574920494E-4	3.7259780142705703E-6	6.334162624259969E-5
86-87	0.39577711065383425	1.490391205708228E-4	2.0120281277061079E-4	3.7259780142705703E-6	6.706760425687026E-5
88-89	0.4854520865122912	1.658060216350404E-4	2.0306580177774608E-4	3.7259780142705703E-6	6.706760425687026E-5
90	0.5633343419555817	1.751209666707168E-4	2.1983270284196366E-4	3.7259780142705703E-6	6.706760425687026E-5
>>END_MODULE
