##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	SRR19654130_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	30435079
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.451612496225163	32.0	32.0	32.0	32.0	32.0
2	31.381332310653768	32.0	32.0	32.0	32.0	32.0
3	34.84702825972622	37.0	32.0	37.0	32.0	37.0
4	35.64891068625122	37.0	37.0	37.0	32.0	37.0
5	36.07291914701454	37.0	37.0	37.0	37.0	37.0
6	38.96171782567083	41.0	37.0	41.0	37.0	41.0
7	39.2493271661953	41.0	41.0	41.0	37.0	41.0
8	39.51935797505241	41.0	41.0	41.0	37.0	41.0
9	39.77592760643073	41.0	41.0	41.0	37.0	41.0
10-11	39.698769436412505	41.0	41.0	41.0	37.0	41.0
12-13	39.62335138016235	41.0	41.0	41.0	37.0	41.0
14-15	39.55966038399309	41.0	41.0	41.0	37.0	41.0
16-17	39.58988782319244	41.0	41.0	41.0	37.0	41.0
18-19	39.59723932374219	41.0	41.0	41.0	37.0	41.0
20-21	39.531256597033966	41.0	41.0	41.0	37.0	41.0
22-23	39.53153330076784	41.0	41.0	41.0	37.0	41.0
24-25	39.470698449640956	41.0	41.0	41.0	37.0	41.0
26-27	39.24395505922623	41.0	41.0	41.0	37.0	41.0
28-29	39.400084389463885	41.0	41.0	41.0	37.0	41.0
30-31	39.471469977127384	41.0	41.0	41.0	37.0	41.0
32-33	39.46669147794885	41.0	41.0	41.0	37.0	41.0
34-35	39.27631349338702	41.0	41.0	41.0	37.0	41.0
36-37	39.195839987798294	41.0	41.0	41.0	37.0	41.0
38-39	39.15466197738471	41.0	41.0	41.0	37.0	41.0
40-41	39.16591888918705	41.0	41.0	41.0	37.0	41.0
42-43	39.141019150960645	41.0	41.0	41.0	37.0	41.0
44-45	39.136002751956056	41.0	41.0	41.0	37.0	41.0
46-47	39.08628352829312	41.0	41.0	41.0	37.0	41.0
48-49	39.06367992341995	41.0	41.0	41.0	37.0	41.0
50-51	39.07280408242082	41.0	41.0	41.0	37.0	41.0
52-53	39.09204173578784	41.0	41.0	41.0	37.0	41.0
54-55	39.093566867363805	41.0	41.0	41.0	37.0	41.0
56-57	39.05249938730239	41.0	41.0	41.0	37.0	41.0
58-59	39.02642587193547	41.0	41.0	41.0	37.0	41.0
60-61	38.986185562390034	41.0	41.0	41.0	37.0	41.0
62-63	38.9250038253556	41.0	41.0	41.0	34.5	41.0
64-65	38.94703567222546	41.0	41.0	41.0	37.0	41.0
66-67	38.93854949415443	41.0	41.0	41.0	37.0	41.0
68-69	38.88671351896278	41.0	41.0	41.0	32.0	41.0
70-71	38.81518497126293	41.0	41.0	41.0	32.0	41.0
72-73	38.826746219387175	41.0	41.0	41.0	32.0	41.0
74-75	38.727532184161575	41.0	41.0	41.0	32.0	41.0
76-77	37.766927383365754	41.0	39.0	41.0	29.5	41.0
78-79	38.32637608727745	41.0	39.0	41.0	32.0	41.0
80-81	38.82297943107031	41.0	41.0	41.0	32.0	41.0
82-83	38.90380880890764	41.0	41.0	41.0	32.0	41.0
84-85	38.87177513158418	41.0	41.0	41.0	32.0	41.0
86-87	38.82498024729951	41.0	41.0	41.0	32.0	41.0
88-89	38.75154822170825	41.0	41.0	41.0	32.0	41.0
90-91	38.633003909731926	41.0	41.0	41.0	32.0	41.0
92-93	38.54653474038953	41.0	41.0	41.0	32.0	41.0
94-95	38.415937888644876	41.0	39.0	41.0	32.0	41.0
96-97	38.44767513171233	41.0	37.0	41.0	32.0	41.0
98-99	38.394983794850674	41.0	37.0	41.0	32.0	41.0
100-101	37.3241972527819	41.0	37.0	41.0	29.5	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	31.0
13	102.0
14	353.0
15	1407.0
16	5553.0
17	14502.0
18	24417.0
19	33807.0
20	43021.0
21	53012.0
22	64965.0
23	78395.0
24	94323.0
25	112077.0
26	132148.0
27	154972.0
28	180999.0
29	212025.0
30	251098.0
31	296978.0
32	353846.0
33	426586.0
34	523932.0
35	662129.0
36	878170.0
37	1267177.0
38	2173445.0
39	5742486.0
40	1.6653123E7
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	34.338310392520356	19.147514615909735	12.97738790114902	33.53678709042089
2	29.67972229295152	22.662833713727164	30.6491326353592	17.008311357962114
3	22.138651793378482	26.138428365485318	28.30560664369412	23.41731319744208
4	25.123850806394643	31.513502295840766	22.429128529428443	20.933518368336145
5	27.336024314112038	33.0053786758666	21.60850008214227	18.050096927879085
6	21.956312938783228	36.320171966685955	21.955800366502324	19.76771472802849
7	21.691292472084598	19.59863485158031	37.319712559313544	21.390360117021544
8	23.230014295216446	23.929805186142964	26.331320701910016	26.508859816730574
9	23.509142197396628	23.450597910391494	28.631051031607313	24.40920886060457
10-11	25.681129331059072	29.572640504728113	22.392342073434406	22.353888090778405
12-13	26.159154459312944	23.98590347128153	25.478256950876993	24.376685118528528
14-15	24.658575717841902	26.740697798090157	26.09997660922779	22.50074987484015
16-17	25.68407141936555	26.197980810910426	25.429183440602465	22.688764329121565
18-19	25.221441022052215	26.79450084555391	25.501098584301356	22.482959548092516
20-21	25.447223101191607	26.800826464325166	25.309811997552355	22.442138436930872
22-23	25.45870097655081	26.635778623600043	25.411544862806	22.493975537043145
24-25	25.014162114873194	26.635809284899693	25.813632843923195	22.53639575630392
26-27	25.202668645193345	26.734943033020937	25.533014363814516	22.529373957971206
28-29	25.53230508971833	26.501247577668423	25.46477050917259	22.501676823440654
30-31	24.997366930862906	26.653314830741586	25.750405811358963	22.59891242703655
32-33	25.118076172300636	26.824261686214694	25.582417965869688	22.475244175614986
34-35	25.42431711775744	26.551371856140083	25.48632779957627	22.537983226526208
36-37	25.09501782466213	26.570882237565407	25.72246321424038	22.61163672353208
38-39	25.258877528923996	26.768890414992452	25.418940974702764	22.553291081380785
40-41	25.554479421591118	26.450793835626317	25.38850810934317	22.606218633439394
42-43	25.168251289430003	26.602942235379125	25.66598488161177	22.562821593579102
44-45	25.284380246261218	26.691933848760936	25.525439138971084	22.498246766006762
46-47	25.463968731607366	26.485911207918996	25.50261164099492	22.54750841947872
48-49	25.093813095881533	26.521172862444466	25.784335113375217	22.60067892829879
50-51	25.38656124051038	26.61459704267159	25.484120937092232	22.5147207797258
52-53	25.553139394160034	26.49734257111473	25.472774336567706	22.47674369815753
54-55	25.14208676626208	26.58103814686174	25.7475294799103	22.529345606965887
56-57	25.13914582395601	26.712817385593056	25.713056012359438	22.4349807780915
58-59	25.54082662754315	26.330790002474792	25.567674102474225	22.560709267507832
60-61	25.18206869119676	26.4919059352532	25.71972459805345	22.606300775496592
62-63	25.283531205099997	26.67556176974008	25.55578129290591	22.485125732254012
64-65	25.44213169820251	26.494724713613753	25.592551883595497	22.470591704588234
66-67	25.11187844448387	26.602759189943793	25.91203593805316	22.37332642751917
68-69	25.405920717566516	26.69758098876797	25.65480541770749	22.241692875958027
70-71	25.65526234183456	26.54680972885812	25.371656873904186	22.42627105540314
72-73	25.174918587195748	26.615305541485508	25.83717222294215	22.372603648376593
74-75	25.21895765647836	26.76236771756616	25.691514144297596	22.327160481657888
76-77	25.61405679059319	26.567430491963684	25.509192221973603	22.30932049546952
78-79	25.225157335738725	26.627909309202952	25.88236131002549	22.26457204503283
80-81	25.380587394569602	26.70514850005589	25.67458893899936	22.239675166375143
82-83	25.64895120982012	26.519476311451236	25.58779485895307	22.24377761977557
84-85	25.333214412870902	26.669020980776416	25.818507484892017	22.17925712146066
86-87	25.398227157550668	26.891599328524823	25.64672823750515	22.06344527641936
88-89	25.670551076933297	26.73395393519432	25.495839849799633	22.099655138072748
90-91	25.374036321706278	26.784341515919834	25.77525755724176	22.066364605132126
92-93	25.57669720522164	26.88545674548767	25.507834232991478	22.03001181629921
94-95	25.90105853232119	26.7011093947064	25.416121174704244	21.981710898268165
96-97	25.55713845318131	26.776603244081432	25.711296822285917	21.954961480451335
98-99	25.76075784130542	27.02544159652091	25.46720020013748	21.74660036203619
100-101	26.14443252721684	26.78386390953211	25.337075677943872	21.73462788530718
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	64.0
1	42.5
2	26.5
3	30.0
4	29.5
5	46.5
6	69.0
7	88.5
8	108.0
9	131.0
10	147.5
11	181.0
12	237.0
13	286.5
14	350.0
15	439.5
16	551.5
17	705.5
18	896.0
19	1139.5
20	1462.0
21	1817.0
22	2374.5
23	3181.5
24	4224.0
25	5668.0
26	7707.5
27	10468.0
28	14362.5
29	19562.0
30	26755.5
31	36950.0
32	51089.5
33	70991.5
34	102542.0
35	151277.5
36	220488.0
37	316288.5
38	454150.0
39	640791.5
40	864809.0
41	1114099.5
42	1382479.0
43	1625119.5
44	1818763.5
45	1955555.0
46	2022065.0
47	2030505.0
48	1969186.0
49	1837227.0
50	1663741.5
51	1495026.0
52	1331499.0
53	1153753.5
54	989406.0
55	844373.5
56	693397.5
57	564951.5
58	470533.5
59	398597.5
60	344533.5
61	296315.0
62	249391.0
63	207429.5
64	182104.0
65	158656.0
66	135183.5
67	115357.0
68	92152.0
69	70459.5
70	60315.0
71	51863.0
72	37429.5
73	25311.5
74	14840.5
75	7670.0
76	4296.0
77	2150.5
78	1266.0
79	843.0
80	623.5
81	476.0
82	386.0
83	316.0
84	264.5
85	212.0
86	181.5
87	169.5
88	136.0
89	120.0
90	101.0
91	82.5
92	75.5
93	64.0
94	62.0
95	65.5
96	59.0
97	60.0
98	73.5
99	120.0
100	583.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.017729541625306773
2	0.007051074189753212
3	6.702791867239773E-4
4	0.0015672704513104764
5	2.5956890074114807E-4
6	0.0011401317538883339
7	0.0
8	0.024373191211365018
9	0.0
10-11	0.0
12-13	0.0039543186334426795
14-15	0.0
16-17	0.0011056320898657763
18-19	0.0
20-21	4.2549585627821106E-4
22-23	3.285682287862634E-6
24-25	1.9714093727175802E-5
26-27	0.00545094691556411
28-29	9.873475275027216E-4
30-31	2.0864082527927724E-4
32-33	5.60208830080579E-4
34-35	0.0
36-37	0.0
38-39	8.953484234425677E-4
40-41	0.0
42-43	7.392785147690926E-5
44-45	0.00199605198987655
46-47	0.0
48-49	0.001628055573635935
50-51	0.001248559269387801
52-53	6.89993280451153E-4
54-55	7.967779548066886E-4
56-57	0.008598630547336512
58-59	6.144225878303125E-4
60-61	0.0
62-63	0.0048628097860366985
64-65	4.9285234317939505E-6
66-67	0.002695902317191291
68-69	3.3678243450591995E-4
70-71	5.585659889366478E-5
72-73	2.0699798413534594E-4
74-75	6.538507752846641E-4
76-77	3.5813936937702706E-4
78-79	2.743544710365299E-4
80-81	0.0017906968468851354
82-83	0.005285019960027047
84-85	5.618516712245104E-4
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	4.5999552030076874E-5
96-97	0.004734668176810055
98-99	0.0
100-101	4.862809786036698E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3.0435079E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.268838485606146
#Duplication Level	Percentage of deduplicated	Percentage of total
1	61.75052831171299	21.16118881114942
2	15.687345207501677	10.751741983676451
3	7.288864429410021	7.493427536247952
4	4.078372678456738	5.590443784085714
5	2.511228663247221	4.302844473062181
6	1.6811017298518698	3.456564219490009
7	1.1633517553848816	2.7906699385061624
8	0.9259838248231221	2.538591210651791
9	0.697432653058575	2.151018624802688
>10	3.835772547106548	23.789537381957068
>50	0.24884483967046014	5.849962489137192
>100	0.12299483166313735	7.563776790162194
>500	0.006218763799452684	1.442264506570364
>1k	0.0019295324838508327	1.0438072241149006
>5k	2.0154552990308798E-5	0.03938908810058074
>10k+	1.0077276495154399E-5	0.034771938285529325
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.9714093727175802E-5	0.0	0.0	3.285682287862634E-6	0.0
2	3.2856822878626335E-5	6.571364575725268E-6	6.571364575725268E-6	6.571364575725268E-6	0.0
3	4.928523431793951E-5	6.571364575725268E-6	1.6428411439313167E-5	9.857046863587901E-6	0.0
4	9.199910406015375E-5	6.571364575725268E-6	3.2856822878626335E-5	1.6428411439313167E-5	6.571364575725268E-6
5	1.0514183321160429E-4	6.571364575725268E-6	3.9428187454351604E-5	1.6428411439313167E-5	6.571364575725268E-6
6	1.4457002066595588E-4	6.571364575725268E-6	4.5999552030076874E-5	1.6428411439313167E-5	6.571364575725268E-6
7	1.5771274981740642E-4	9.857046863587901E-6	6.242796346939005E-5	1.6428411439313167E-5	9.857046863587901E-6
8	1.839982081203075E-4	9.857046863587901E-6	7.228501033297794E-5	1.6428411439313167E-5	2.2999776015038437E-5
9	2.0042661955962067E-4	9.857046863587901E-6	7.885637490870321E-5	1.6428411439313167E-5	2.2999776015038437E-5
10-11	2.743544710365299E-4	9.857046863587901E-6	8.871342177229111E-5	1.9714093727175802E-5	2.2999776015038437E-5
12-13	3.26925387642332E-4	9.857046863587901E-6	1.0021330977981034E-4	2.6285458302901072E-5	2.2999776015038437E-5
14-15	3.7621062196027157E-4	9.857046863587901E-6	1.281416092266427E-4	2.9571140590763703E-5	2.2999776015038437E-5
16-17	4.4685279114931823E-4	9.857046863587901E-6	2.4149764815790356E-4	3.614250516648897E-5	2.2999776015038437E-5
18-19	5.569231477927164E-4	9.857046863587901E-6	2.546403773093541E-4	3.9428187454351604E-5	2.2999776015038437E-5
20-21	7.244929444737107E-4	2.6285458302901072E-5	2.907828824758431E-4	4.107102859828292E-5	2.2999776015038437E-5
22-23	9.117768348818809E-4	2.6285458302901072E-5	3.3842527564985125E-4	4.271386974221424E-5	2.2999776015038437E-5
24-25	0.001307701550569328	2.9571140590763703E-5	3.449966402255765E-4	4.271386974221424E-5	2.2999776015038437E-5
26-27	0.0018596961749302507	3.2856822878626335E-5	3.5485368708916445E-4	4.764239317400819E-5	2.4642617158969754E-5
28-29	0.002502047062207396	3.449966402255765E-5	3.8113914539206553E-4	5.421375774973346E-5	2.9571140590763703E-5
30-31	0.0031411122671966777	4.271386974221424E-5	3.893533511117221E-4	5.585659889366477E-5	3.449966402255765E-5
32-33	0.0038803907819657704	4.271386974221424E-5	3.9428187454351607E-4	5.585659889366477E-5	3.614250516648897E-5
34-35	0.004795453299135514	4.271386974221424E-5	4.7478109059615056E-4	5.585659889366477E-5	4.107102859828292E-5
36-37	0.005930656529592054	4.271386974221424E-5	4.961380254672578E-4	5.585659889366477E-5	4.928523431793951E-5
38-39	0.007238358080161382	4.764239317400819E-5	5.158521191944334E-4	5.749944003759609E-5	4.928523431793951E-5
40-41	0.008966626963577126	5.0928075461870826E-5	5.470661009291285E-4	5.9142281181527407E-5	4.928523431793951E-5
42-43	0.011171319778732956	5.9142281181527407E-5	5.815657649516862E-4	5.9142281181527407E-5	6.078512232545873E-5
44-45	0.013666795476364626	6.407080461332137E-5	5.96351335247068E-4	5.9142281181527407E-5	6.7356486901184E-5
46-47	0.016311769718094046	8.214205719656584E-5	6.571364575725267E-4	5.9142281181527407E-5	7.228501033297794E-5
48-49	0.019727236456327252	8.214205719656584E-5	6.768505512997025E-4	5.9142281181527407E-5	7.228501033297794E-5
50-51	0.02446519031542517	8.214205719656584E-5	6.850647570193591E-4	6.242796346939005E-5	7.228501033297794E-5
52-53	0.03082791406587116	8.214205719656584E-5	7.162787387540541E-4	6.242796346939005E-5	7.557069262084057E-5
54-55	0.03784284575045789	8.542773948442848E-5	7.442070382008866E-4	6.242796346939005E-5	8.70705806283598E-5
56-57	0.04568576937158599	9.199910406015375E-5	7.885637490870321E-4	6.242796346939005E-5	8.871342177229111E-5
58-59	0.05515017720177431	9.528478634801638E-5	8.115635251020705E-4	6.242796346939005E-5	8.871342177229111E-5
60-61	0.06718563142221513	9.857046863587902E-5	8.690629651396666E-4	6.242796346939005E-5	8.871342177229111E-5
62-63	0.08313761892978822	1.0021330977981034E-4	9.544907046240951E-4	6.571364575725267E-5	9.035626291622243E-5
64-65	0.10286485538611548	1.0514183321160429E-4	0.0010021330977981035	6.571364575725267E-5	9.364194520408507E-5
66-67	0.12622277077053093	1.1171319778732954E-4	0.0010070616212298972	6.571364575725267E-5	9.528478634801638E-5
68-69	0.15442871037068773	1.166417212191235E-4	0.0010300613972449358	6.571364575725267E-5	9.528478634801638E-5
70-71	0.18888566052350317	1.281416092266427E-4	0.0010382756029645923	7.228501033297794E-5	1.0185615092374165E-4
72-73	0.23262137745724265	1.3142729151450534E-4	0.001067846743555356	7.885637490870321E-5	1.0514183321160429E-4
74-75	0.28984646302380224	1.3307013265843664E-4	0.001067846743555356	8.214205719656584E-5	1.0678467435553559E-4
76-77	0.3628033953846481	1.379986560902306E-4	0.00107277526698715	8.214205719656584E-5	1.1007035664339823E-4
78-79	0.45191438471377054	1.4128433837809324E-4	0.0010777037904189438	8.214205719656584E-5	1.1171319778732954E-4
80-81	0.5613621045636188	1.4949854409774984E-4	0.0010809894727068065	8.214205719656584E-5	1.1499888007519218E-4
82-83	0.6954261561141339	1.5114138524168115E-4	0.001099060725290051	8.214205719656584E-5	1.1499888007519218E-4
84-85	0.8619379630984365	1.5606990867347511E-4	0.0011072749310097076	8.542773948442848E-5	1.166417212191235E-4
86-87	1.0732615479657537	1.5771274981740642E-4	0.0011302747070247462	8.70705806283598E-5	1.1828456236305481E-4
88-89	1.3319745284709135	1.642841143931317E-4	0.0011434174361761966	9.364194520408507E-5	1.1828456236305481E-4
90	1.5560991315317434	1.9056957269603277E-4	0.0011532744830397845	9.857046863587902E-5	1.1828456236305481E-4
>>END_MODULE
