##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	SRR19654134_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	33930019
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.62249328537069	32.0	32.0	32.0	32.0	32.0
2	31.69592206830182	32.0	32.0	32.0	32.0	32.0
3	35.75789164751131	37.0	37.0	37.0	32.0	37.0
4	36.36553248025001	37.0	37.0	37.0	37.0	37.0
5	36.44318725550964	37.0	37.0	37.0	37.0	37.0
6	40.10345237354568	41.0	41.0	41.0	37.0	41.0
7	40.11618086627066	41.0	41.0	41.0	37.0	41.0
8	40.25494332909156	41.0	41.0	41.0	41.0	41.0
9	40.230401904578954	41.0	41.0	41.0	41.0	41.0
10-11	40.293960858082635	41.0	41.0	41.0	41.0	41.0
12-13	40.253139130278704	41.0	41.0	41.0	41.0	41.0
14-15	40.28557167916705	41.0	41.0	41.0	41.0	41.0
16-17	40.287511878493206	41.0	41.0	41.0	41.0	41.0
18-19	40.28437461234549	41.0	41.0	41.0	41.0	41.0
20-21	40.21538542315582	41.0	41.0	41.0	41.0	41.0
22-23	40.1744634891009	41.0	41.0	41.0	39.0	41.0
24-25	40.2114594011869	41.0	41.0	41.0	41.0	41.0
26-27	40.18656197923143	41.0	41.0	41.0	41.0	41.0
28-29	40.1063882251289	41.0	41.0	41.0	39.0	41.0
30-31	40.12035893643325	41.0	41.0	41.0	39.0	41.0
32-33	40.13184201282056	41.0	41.0	41.0	41.0	41.0
34-35	40.12781177635061	41.0	41.0	41.0	41.0	41.0
36-37	40.10705962174675	41.0	41.0	41.0	37.0	41.0
38-39	40.08448305024527	41.0	41.0	41.0	37.0	41.0
40-41	40.07221820300189	41.0	41.0	41.0	37.0	41.0
42-43	40.0225403646252	41.0	41.0	41.0	37.0	41.0
44-45	40.03851390416257	41.0	41.0	41.0	37.0	41.0
46-47	40.02572011527609	41.0	41.0	41.0	37.0	41.0
48-49	40.001602754775945	41.0	41.0	41.0	37.0	41.0
50-51	39.93522214060653	41.0	41.0	41.0	37.0	41.0
52-53	39.95762744783609	41.0	41.0	41.0	37.0	41.0
54-55	39.90539927195443	41.0	41.0	41.0	37.0	41.0
56-57	39.9041153498912	41.0	41.0	41.0	37.0	41.0
58-59	39.895515443124275	41.0	41.0	41.0	37.0	41.0
60-61	39.82493032202546	41.0	41.0	41.0	37.0	41.0
62-63	39.784636298022704	41.0	41.0	41.0	37.0	41.0
64-65	39.77797687056998	41.0	41.0	41.0	37.0	41.0
66-67	39.74601589524604	41.0	41.0	41.0	37.0	41.0
68-69	39.665295722351345	41.0	41.0	41.0	37.0	41.0
70-71	39.6878185214102	41.0	41.0	41.0	37.0	41.0
72-73	39.6044564401806	41.0	41.0	41.0	37.0	41.0
74-75	39.6438714637914	41.0	41.0	41.0	37.0	41.0
76-77	39.10925469861954	41.0	39.0	41.0	34.5	41.0
78-79	39.42340553950176	41.0	41.0	41.0	37.0	41.0
80-81	39.62836584323752	41.0	41.0	41.0	37.0	41.0
82-83	39.76793588002411	41.0	41.0	41.0	37.0	41.0
84-85	39.72735619452497	41.0	41.0	41.0	37.0	41.0
86-87	39.74669663462316	41.0	41.0	41.0	37.0	41.0
88-89	39.68582239815427	41.0	41.0	41.0	37.0	41.0
90-91	39.7026271485436	41.0	41.0	41.0	37.0	41.0
92-93	39.658006749126784	41.0	41.0	41.0	37.0	41.0
94-95	39.519314106484885	41.0	41.0	41.0	37.0	41.0
96-97	39.48630299912299	41.0	41.0	41.0	37.0	41.0
98-99	39.540334740160326	41.0	41.0	41.0	37.0	41.0
100-101	39.00343683862953	41.0	39.0	41.0	34.5	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	3.0
17	15.0
18	48.0
19	154.0
20	540.0
21	1579.0
22	3726.0
23	7970.0
24	15226.0
25	26959.0
26	45282.0
27	70580.0
28	104427.0
29	144535.0
30	191922.0
31	243927.0
32	302019.0
33	366630.0
34	440349.0
35	541772.0
36	686817.0
37	935870.0
38	1479331.0
39	3366714.0
40	2.4953624E7
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.20448255299342	12.130388151075287	5.483526456478288	40.18160283945301
2	24.8847871261139	13.951327289265592	31.474055466930327	29.68983011769018
3	20.810047291750706	17.099006045354706	22.2309778252703	39.85996883762429
4	24.00508475845334	24.78797342549564	21.52144090048675	29.685500915564273
5	24.761801636480072	29.324495810037714	23.123791943647305	22.789910609834905
6	20.40061928642009	32.2740727024055	24.72828559276669	22.59702241840772
7	16.017465242209266	24.905043525027203	40.51455438324393	18.562936849519595
8	18.736670910794363	24.396594060262682	31.083979057011433	25.782755971931522
9	18.769907339045837	22.66675154962986	33.819226403788846	24.744114707535456
10-11	21.76799537856426	31.258564613890538	24.510400440922695	22.463039566622506
12-13	21.954548549871408	25.273642387994045	27.42362672387635	25.3481823382582
14-15	21.36835084000395	26.21101538434152	27.326829672568113	25.093804103086413
16-17	22.010474851701268	26.53818557792978	26.760156034791954	24.691183535576997
18-19	21.796098226218895	26.78733400679823	26.489585736565196	24.92698203041768
20-21	21.904606486316137	26.76730482590631	26.640421187131768	24.68766750064578
22-23	21.927447196537084	26.92590888322226	26.542954485230318	24.603689435010338
24-25	21.92532352695255	26.61838188881544	26.34885653019272	25.10743805403929
26-27	21.65971083010593	26.841575007665043	26.58808266508781	24.91063149714122
28-29	21.69523994611015	27.03641313727765	26.482160953248624	24.78618596336358
30-31	21.858589292272427	26.726588334654338	26.50202465256503	24.912797720508202
32-33	21.90976577833455	26.628968411654768	26.573703547698628	24.88756226231205
34-35	22.08955724474768	26.76734238772615	26.289652093442616	24.85344827408355
36-37	21.86320334107744	26.673995111036746	26.26156822834661	25.201233319539202
38-39	21.832527553291744	26.72504983551642	26.481509088464385	24.960913522727456
40-41	22.040413113450644	27.04764837690915	26.3718614925496	24.54007701709061
42-43	22.024544106503445	26.724451583714114	26.360683735544033	24.890320574238405
44-45	22.023877820735496	26.654992758897812	26.460246206021733	24.860883214344966
46-47	22.02231691796576	26.682162610653705	26.44301109279429	24.852509378586248
48-49	21.857950988901496	26.583461749037436	26.405874733499747	25.15271252856132
50-51	21.85988694933572	26.531959165291692	26.62014658793852	24.98800729743407
52-53	22.24633885409849	26.682892514737468	26.27427058027878	24.796498050885265
54-55	22.0916803294669	26.6642319281081	26.273786630900975	24.970301111524023
56-57	21.97611049093566	26.462501106508828	26.60163496727939	24.95975343527612
58-59	22.034819300081114	26.59320503294238	26.524299476260886	24.84767619071562
60-61	22.084242386339074	26.442129469032093	26.460048715593793	25.013579429035037
62-63	22.031193852564797	26.401127205639803	26.56265572077272	25.005023221022682
64-65	22.258453769547042	26.564320421327853	26.40891934646931	24.76830646265579
66-67	22.071128663534047	26.39919476195024	26.40444832630208	25.12522824821363
68-69	22.079207089073822	26.40654502560487	26.64339375497021	24.8708541303511
70-71	22.302916511769542	26.660645420860252	26.46951053490813	24.566927532462078
72-73	22.30222415208881	26.45766783255896	26.28559346484926	24.954514550502964
74-75	22.213305883946727	26.414518285561208	26.49974623189249	24.87242959859958
76-77	22.16120774939737	26.654008062889677	26.413373066487228	24.77141112122572
78-79	22.131999920953948	26.45244724969181	26.367102860678663	25.04844996867557
80-81	22.13942384249363	26.461839405799374	26.505748309841	24.892988441866
82-83	22.33379231443576	26.699718277346275	26.31520751458476	24.6512818936332
84-85	22.394654120280023	26.55717038926178	26.175029007899695	24.8731464825585
86-87	22.322778058113443	26.47245233793829	26.42740360249286	24.777366001455405
88-89	22.499972922166815	26.50093755615887	26.370650075040597	24.628439446633717
90-91	22.381774328577805	26.405367811316978	26.30778691622886	24.90507094387636
92-93	22.376333827019142	26.38104508212424	26.275480099919207	24.96714099093741
94-95	22.536660936752977	26.51451201735089	26.169048818360608	24.77977822753553
96-97	22.522614870420476	26.505228492375966	25.993101435186546	24.97905520201702
98-99	22.494794379750058	26.584405333529503	26.217375230933754	24.70342505578669
100-101	22.648038206473732	26.674242164755245	26.088751736860218	24.5889678919108
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	54.0
1	43.5
2	24.0
3	19.0
4	30.5
5	42.0
6	51.5
7	61.0
8	74.0
9	105.5
10	131.5
11	158.5
12	180.0
13	199.5
14	242.5
15	280.5
16	342.0
17	414.0
18	511.5
19	677.0
20	904.0
21	1255.5
22	1864.5
23	2832.0
24	4282.0
25	6349.5
26	9410.5
27	14226.0
28	20916.0
29	30123.0
30	43359.5
31	60949.0
32	87394.0
33	122797.5
34	167161.0
35	233978.5
36	327823.5
37	452766.0
38	618783.0
39	836300.0
40	1081724.0
41	1324570.0
42	1568321.5
43	1804680.5
44	2000725.0
45	2119159.0
46	2178401.0
47	2193620.5
48	2124751.0
49	1994074.5
50	1830786.5
51	1626432.0
52	1451198.5
53	1303732.5
54	1134440.5
55	963737.0
56	801853.5
57	646910.0
58	511651.0
59	404311.0
60	324524.0
61	275911.0
62	237718.5
63	200688.0
64	167334.5
65	141533.5
66	122900.5
67	98904.5
68	69297.5
69	46817.5
70	33910.0
71	25983.0
72	20944.5
73	17912.0
74	14486.5
75	9946.5
76	6130.5
77	2382.0
78	342.0
79	94.5
80	35.5
81	18.0
82	10.0
83	6.5
84	4.0
85	3.5
86	2.0
87	0.5
88	1.0
89	0.5
90	0.0
91	0.0
92	1.5
93	1.5
94	0.0
95	1.0
96	1.0
97	0.0
98	0.0
99	0.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03518123582542055
2	0.0
3	0.0
4	0.019678739348775492
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0027969332996836812
10-11	0.0034453266884406993
12-13	0.0016578240053446477
14-15	0.0
16-17	8.208070853128612E-4
18-19	4.4208640142523944E-5
20-21	3.315648010689295E-4
22-23	0.0
24-25	5.894485352336526E-6
26-27	0.0
28-29	1.974652593032736E-4
30-31	0.0
32-33	0.002020334854513344
34-35	5.599761084719699E-4
36-37	3.713525771972011E-4
38-39	0.00414087596001641
40-41	5.15767468329446E-5
42-43	0.0
44-45	0.002334216199525264
46-47	1.915707739509371E-4
48-49	5.113466043151936E-4
50-51	3.094604809976676E-5
52-53	0.0
54-55	8.782783174981424E-4
56-57	3.079868596595835E-4
58-59	7.206008343231403E-4
60-61	6.189209619953352E-5
62-63	2.8440891825023734E-4
64-65	0.002113172998812644
66-67	0.0019849679423993246
68-69	0.008485111664688428
70-71	0.008639841905187262
72-73	6.262890686857558E-4
74-75	0.0012393155453287543
76-77	0.0
78-79	0.0012687879720904372
80-81	0.008722364700119972
82-83	0.005806068072051478
84-85	1.1052160035630985E-4
86-87	0.010104621515242888
88-89	1.5178299782266552E-4
90-91	0.002163276124307505
92-93	6.100792339668303E-4
94-95	0.014272022659344812
96-97	0.0010403766646873967
98-99	3.079868596595835E-4
100-101	0.015611544455663286
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3.3930019E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.151926876031837
#Duplication Level	Percentage of deduplicated	Percentage of total
1	63.50176040510276	19.782021966390552
2	15.08773136087541	9.400238081584062
3	6.463534675505332	6.04054678716115
4	3.6064630324590197	4.493930906731017
5	2.4151308935058986	3.7617990495270592
6	1.6073839181280427	3.0043863767540597
7	1.1229086640542851	2.4486538013757184
8	0.9120095798356638	2.2728684593024897
9	0.6593078927594955	1.8484840137631002
>10	4.121941387675447	23.77541964184877
>50	0.3142661041672539	6.711243276026643
>100	0.16756013510661366	9.684811315388236
>500	0.013005647868783414	2.770574550189714
>1k	0.0069170123907217485	3.8209348324318744
>5k	5.946792399944335E-5	0.11266533320358975
>10k+	1.9822641333147783E-5	0.07142160832188102
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.957555225654309E-5	0.0	0.0	0.0	0.0
2	1.0904797901822572E-4	0.0	0.0	2.947242676168263E-6	1.1788970704673051E-5
3	1.3852040577990834E-4	2.947242676168263E-6	0.0	2.947242676168263E-6	2.3577941409346103E-5
4	2.6525184085514364E-4	2.947242676168263E-6	5.894485352336526E-6	2.947242676168263E-6	2.6525184085514364E-5
5	3.0061875296916277E-4	2.947242676168263E-6	5.894485352336526E-6	2.947242676168263E-6	2.6525184085514364E-5
6	3.654580918448646E-4	2.947242676168263E-6	1.1788970704673051E-5	2.947242676168263E-6	2.6525184085514364E-5
7	4.1556121733972505E-4	1.4736213380841314E-5	1.7683456057009577E-5	2.947242676168263E-6	2.9472426761682628E-5
8	4.509281294537442E-4	1.4736213380841314E-5	1.7683456057009577E-5	2.947242676168263E-6	2.9472426761682628E-5
9	4.892422842439316E-4	1.4736213380841314E-5	2.0630698733177838E-5	2.947242676168263E-6	3.241966943785089E-5
10-11	5.791331858670636E-4	1.4736213380841314E-5	2.0630698733177838E-5	2.947242676168263E-6	3.5366912114019154E-5
12-13	6.528142527712701E-4	2.9472426761682628E-5	2.0630698733177838E-5	4.420864014252394E-6	4.1261397466355676E-5
14-15	7.648094744656642E-4	2.9472426761682628E-5	2.0630698733177838E-5	5.894485352336526E-6	4.1261397466355676E-5
16-17	8.797519388362264E-4	3.094604809976676E-5	2.0630698733177838E-5	5.894485352336526E-6	4.2735018804439807E-5
18-19	9.401704136976758E-4	3.241966943785089E-5	2.0630698733177838E-5	5.894485352336526E-6	4.420864014252394E-5
20-21	0.0010374294220112283	3.241966943785089E-5	2.0630698733177838E-5	5.894485352336526E-6	4.8629504156776336E-5
22-23	0.0011494246437056225	3.5366912114019154E-5	2.0630698733177838E-5	5.894485352336526E-6	5.0103125494860466E-5
24-25	0.0012953131561759515	5.599761084719699E-5	2.0630698733177838E-5	5.894485352336526E-6	5.305036817102873E-5
26-27	0.0014677268527317948	8.989090162313201E-5	2.0630698733177838E-5	5.894485352336526E-6	5.305036817102873E-5
28-29	0.001693190917458667	9.136452296121615E-5	3.094604809976676E-5	5.894485352336526E-6	5.305036817102873E-5
30-31	0.0020910686787413823	9.136452296121615E-5	3.241966943785089E-5	5.894485352336526E-6	5.452398950911286E-5
32-33	0.0026893589420035396	9.725900831355267E-5	3.241966943785089E-5	5.894485352336526E-6	5.599761084719699E-5
34-35	0.0035823734728825233	1.0610073634205745E-4	3.241966943785089E-5	5.894485352336526E-6	6.041847486144939E-5
36-37	0.00481874177553511	1.0757435768014159E-4	3.241966943785089E-5	5.894485352336526E-6	6.189209619953351E-5
38-39	0.006562035818488637	1.1199522169439398E-4	3.241966943785089E-5	5.894485352336526E-6	7.368106690420657E-5
40-41	0.008905093746042406	1.1199522169439398E-4	3.241966943785089E-5	5.894485352336526E-6	8.252279493271135E-5
42-43	0.012014434769399923	1.178897070467305E-4	3.241966943785089E-5	5.894485352336526E-6	8.252279493271135E-5
44-45	0.015334503644103471	1.3115229908948768E-4	3.5366912114019154E-5	5.894485352336526E-6	8.841728028504787E-5
46-47	0.01938254145982058	1.4736213380841314E-4	3.5366912114019154E-5	5.894485352336526E-6	9.136452296121615E-5
48-49	0.02448716577494401	1.4736213380841314E-4	3.5366912114019154E-5	5.894485352336526E-6	1.0020625098972093E-4
50-51	0.03160328321655228	1.4736213380841314E-4	3.5366912114019154E-5	5.894485352336526E-6	1.0610073634205745E-4
52-53	0.04017828578286384	1.547302404988338E-4	3.6840533452103284E-5	5.894485352336526E-6	1.0610073634205745E-4
54-55	0.05017091207641233	1.5915110451308618E-4	3.8314154790187415E-5	5.894485352336526E-6	1.1052160035630984E-4
56-57	0.061088972570277664	1.8125542458434816E-4	5.15767468329446E-5	8.841728028504788E-6	1.1494246437056225E-4
58-59	0.07406715569478461	1.9009715261285293E-4	6.189209619953351E-5	8.841728028504788E-6	1.2083694972289877E-4
60-61	0.09008984050377336	1.9893888064135773E-4	6.483933887570179E-5	8.841728028504788E-6	1.2083694972289877E-4
62-63	0.11027845283552598	2.0041250197944186E-4	6.483933887570179E-5	8.841728028504788E-6	1.2083694972289877E-4
64-65	0.13446352623616276	2.0483336599369425E-4	6.631296021378591E-5	8.841728028504788E-6	1.2231057106098288E-4
66-67	0.1625198028919465	2.1367509402219905E-4	6.778658155187005E-5	8.841728028504788E-6	1.2378419239906702E-4
68-69	0.19506620376487263	2.1514871536028318E-4	7.073382422803831E-5	8.841728028504788E-6	1.2525781373715116E-4
70-71	0.2344059990063666	2.1514871536028318E-4	7.073382422803831E-5	8.841728028504788E-6	1.2967867775140357E-4
72-73	0.28292056069877236	2.2399044338878795E-4	7.662830958037483E-5	8.841728028504788E-6	1.2967867775140357E-4
74-75	0.34342892646184486	2.343057927553769E-4	7.662830958037483E-5	8.841728028504788E-6	1.2967867775140357E-4
76-77	0.41584267901529914	2.681990835313119E-4	7.662830958037483E-5	8.841728028504788E-6	1.370467844418242E-4
78-79	0.5006156937312649	2.69672704869396E-4	7.662830958037483E-5	8.841728028504788E-6	1.3999402711799248E-4
80-81	0.6016781187184128	2.711463262074802E-4	7.662830958037483E-5	8.841728028504788E-6	1.4441489113224486E-4
82-83	0.725413681613323	2.726199475455643E-4	7.662830958037483E-5	8.841728028504788E-6	1.4736213380841314E-4
84-85	0.8771568916598602	2.7998805423598496E-4	7.662830958037483E-5	8.841728028504788E-6	1.4736213380841314E-4
86-87	1.0610471511967028	2.91777024940658E-4	7.810193091845895E-5	8.841728028504788E-6	1.4736213380841314E-4
88-89	1.2759954540550065	3.109341023357517E-4	8.252279493271135E-5	8.841728028504788E-6	1.5767748317500207E-4
90	1.458112947122134	3.2124945170234064E-4	8.841728028504787E-5	8.841728028504788E-6	1.650455898654227E-4
>>END_MODULE
