##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	SRR19654112_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	35332753
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.466524445462827	32.0	32.0	32.0	32.0	32.0
2	31.37017927813324	32.0	32.0	32.0	32.0	32.0
3	34.98844941406066	37.0	32.0	37.0	32.0	37.0
4	35.83569163150123	37.0	37.0	37.0	32.0	37.0
5	36.11342147610179	37.0	37.0	37.0	37.0	37.0
6	38.90183940662648	41.0	37.0	41.0	37.0	41.0
7	39.218890132903034	41.0	41.0	41.0	37.0	41.0
8	39.50378992545529	41.0	41.0	41.0	37.0	41.0
9	39.79615967088667	41.0	41.0	41.0	37.0	41.0
10-11	39.75870641894222	41.0	41.0	41.0	37.0	41.0
12-13	39.66668745002689	41.0	41.0	41.0	37.0	41.0
14-15	39.607791204381954	41.0	41.0	41.0	37.0	41.0
16-17	39.630681141659124	41.0	41.0	41.0	37.0	41.0
18-19	39.635293873081444	41.0	41.0	41.0	37.0	41.0
20-21	39.57475157681599	41.0	41.0	41.0	37.0	41.0
22-23	39.597016584017666	41.0	41.0	41.0	37.0	41.0
24-25	39.54476602771372	41.0	41.0	41.0	37.0	41.0
26-27	39.328810197722206	41.0	41.0	41.0	37.0	41.0
28-29	39.44417468686915	41.0	41.0	41.0	37.0	41.0
30-31	39.525604882812274	41.0	41.0	41.0	37.0	41.0
32-33	39.5178482129598	41.0	41.0	41.0	37.0	41.0
34-35	39.32644222486711	41.0	41.0	41.0	37.0	41.0
36-37	39.24456084698523	41.0	41.0	41.0	37.0	41.0
38-39	39.20661962004489	41.0	41.0	41.0	37.0	41.0
40-41	39.21869766276067	41.0	41.0	41.0	37.0	41.0
42-43	39.19013192943103	41.0	41.0	41.0	37.0	41.0
44-45	39.18477231309997	41.0	41.0	41.0	37.0	41.0
46-47	39.13931266550331	41.0	41.0	41.0	37.0	41.0
48-49	39.116979095854774	41.0	41.0	41.0	37.0	41.0
50-51	39.1269933169374	41.0	41.0	41.0	37.0	41.0
52-53	39.14472125905389	41.0	41.0	41.0	37.0	41.0
54-55	39.14626002961049	41.0	41.0	41.0	37.0	41.0
56-57	39.10799622661727	41.0	41.0	41.0	37.0	41.0
58-59	39.08242953782854	41.0	41.0	41.0	37.0	41.0
60-61	39.03974158198202	41.0	41.0	41.0	37.0	41.0
62-63	38.97821947245379	41.0	41.0	41.0	37.0	41.0
64-65	39.0010889046772	41.0	41.0	41.0	37.0	41.0
66-67	38.99256358540757	41.0	41.0	41.0	37.0	41.0
68-69	38.94052877793021	41.0	41.0	41.0	37.0	41.0
70-71	38.86719357815113	41.0	41.0	41.0	32.0	41.0
72-73	38.87692804463892	41.0	41.0	41.0	32.0	41.0
74-75	38.780082718151064	41.0	41.0	41.0	32.0	41.0
76-77	37.83521660483122	41.0	39.0	41.0	29.5	41.0
78-79	38.390109426231234	41.0	39.0	41.0	32.0	41.0
80-81	38.87763154204259	41.0	41.0	41.0	32.0	41.0
82-83	38.95659036248888	41.0	41.0	41.0	37.0	41.0
84-85	38.92184993340315	41.0	41.0	41.0	34.5	41.0
86-87	38.87716431267046	41.0	41.0	41.0	32.0	41.0
88-89	38.804638050706096	41.0	41.0	41.0	32.0	41.0
90-91	38.688739496183615	41.0	41.0	41.0	32.0	41.0
92-93	38.605941674004285	41.0	41.0	41.0	32.0	41.0
94-95	38.46824013401956	41.0	39.0	41.0	32.0	41.0
96-97	38.501667150023664	41.0	37.0	41.0	32.0	41.0
98-99	38.44822263071321	41.0	37.0	41.0	32.0	41.0
100-101	37.37114410530082	41.0	37.0	41.0	29.5	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	25.0
13	103.0
14	383.0
15	1570.0
16	6211.0
17	15831.0
18	27658.0
19	38150.0
20	48187.0
21	59265.0
22	72517.0
23	88148.0
24	105156.0
25	125050.0
26	146483.0
27	172048.0
28	201785.0
29	235534.0
30	277921.0
31	329936.0
32	396396.0
33	475978.0
34	589012.0
35	748651.0
36	998661.0
37	1449907.0
38	2494501.0
39	6621957.0
40	1.9605729E7
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	33.48684606270733	18.344964593855124	14.106601857897308	34.061587485540244
2	29.6361315462641	22.050259772603834	30.61144388631828	17.702164794813793
3	22.32802655049946	25.507825397555873	28.461823751140518	23.702324300804147
4	25.28546250826052	30.721910704571293	22.604830157445797	21.38779662972239
5	27.451408202739103	32.60818085566779	21.83284185700024	18.107569084592864
6	21.733808546639306	36.45164045725356	21.921537387952522	19.893013608154615
7	21.653336777918213	19.511952550088584	37.42790152808076	21.406809143912447
8	23.107412949089255	23.95449761458245	26.459094036742048	26.478995399586243
9	23.502029405973545	23.24673370342809	28.7229274209117	24.52830946968667
10-11	25.45043829446293	29.59460871899792	22.537012612631685	22.41794037390746
12-13	26.140853602904844	23.840063528707876	25.538855582926683	24.480227285460593
14-15	24.450374699078782	26.65804303446154	26.219994801990097	22.67158746446958
16-17	25.5346355001742	26.0705318830682	25.537717657933694	22.857114958823903
18-19	25.07149952340255	26.73207774101271	25.601039352919937	22.595383382664807
20-21	25.30341541153886	26.6843473992244	25.385670837604234	22.62656635163251
22-23	25.312217060486137	26.490519044080646	25.51959831148724	22.677665583945977
24-25	24.79701548229597	26.570200718064807	25.946572391886686	22.686211407752538
26-27	25.094319497514416	26.664315935046503	25.61247580708126	22.628888760357825
28-29	25.3740071898296	26.41682173440254	25.519789777141998	22.68938129862586
30-31	24.871281716508125	26.532395238748418	25.874310512692578	22.722012532050883
32-33	24.957154979100448	26.790140441779837	25.679096376129035	22.573608202990687
34-35	25.247538735518287	26.49328513971159	25.585770234207338	22.673405890562787
36-37	24.914506378826466	26.47458153062684	25.901607497157098	22.709304593389597
38-39	25.10129858323638	26.708465189302437	25.573454063970523	22.616782163490655
40-41	25.396539295989758	26.388315962812182	25.466416387084244	22.74872835411382
42-43	24.927219749868755	26.572466192890666	25.844137267509833	22.656176789730743
44-45	25.110241523698512	26.630521329460876	25.68484678718971	22.574390359650902
46-47	25.319660203098245	26.423818432715958	25.601499266134176	22.65502209805163
48-49	24.877730871086538	26.449672262850648	25.931244335284344	22.741352530778475
50-51	25.198428458636247	26.54786297140393	25.588852879835777	22.664855690124046
52-53	25.413820113843055	26.433729487937196	25.593419622533382	22.559030775686363
54-55	24.925783912487855	26.512636005396452	25.896125143255276	22.665454938860414
56-57	24.968026598202	26.683368168699378	25.83604277034856	22.51256246275006
58-59	25.35314448271088	26.303989254547687	25.68058071473713	22.6622855480043
60-61	25.052154866053034	26.434404927348854	25.840330075610012	22.673110130988093
62-63	25.13671370646443	26.614751127977677	25.677621920022425	22.570913245535465
64-65	25.311376480582798	26.44150502803341	25.6964724554914	22.550646035892395
66-67	24.95891626015493	26.573316013147984	25.97174746302555	22.496020263671532
68-69	25.238187692739633	26.75980198479366	25.690547040923068	22.311463281543638
70-71	25.57485361874346	26.521242313147912	25.413517391549718	22.49038667655891
72-73	25.07675967187432	26.584781381293993	25.910913751492565	22.427545195339125
74-75	25.072924629551487	26.79890010176596	25.684123232403195	22.44405203627936
76-77	25.487082670307192	26.571121655628737	25.531690160128605	22.41010551393547
78-79	25.09626680684669	26.67392888022319	25.885625683760054	22.344178629170067
80-81	25.34527885319568	26.73769559409954	25.669697444370076	22.247328108334706
82-83	25.60693981509818	26.488411529138965	25.579071868013596	22.325576787749256
84-85	25.31315918767764	26.647822286359496	25.815684486623304	22.223334039339562
86-87	25.39493879800422	26.89802574964934	25.543458218497722	22.16357723384872
88-89	25.669199906387142	26.731592355681993	25.418545789511505	22.18066194841936
90-91	25.333947230208754	26.795995771968293	25.687079917039014	22.18297708078394
92-93	25.633172427860345	26.915176974746352	25.39216658266057	22.059484014732732
94-95	26.020962348211384	26.734445275889446	25.247318488447984	21.997273887451183
96-97	25.66921610650924	26.820959367675407	25.565369903839237	21.944454621976117
98-99	25.959282029339747	27.0874576345636	25.2161938810712	21.737066455025456
100-101	26.329689496364704	26.831852068323965	25.106372428518988	21.732086006792343
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	52.0
1	36.5
2	20.0
3	22.0
4	31.5
5	35.5
6	46.0
7	68.0
8	81.5
9	103.5
10	133.0
11	161.0
12	195.0
13	235.5
14	300.0
15	372.0
16	468.5
17	570.5
18	702.0
19	904.0
20	1131.5
21	1475.0
22	2001.5
23	2629.0
24	3487.5
25	4795.5
26	6596.0
27	8934.5
28	12391.5
29	17235.0
30	23935.5
31	33519.0
32	48025.0
33	69463.5
34	103262.0
35	159558.0
36	242812.0
37	361636.5
38	534266.0
39	765432.0
40	1033306.0
41	1316853.5
42	1624335.5
43	1897027.5
44	2103012.0
45	2243988.0
46	2305927.0
47	2313023.0
48	2251925.0
49	2116328.0
50	1928826.0
51	1749079.5
52	1579217.0
53	1394289.5
54	1216687.0
55	1044586.5
56	865001.5
57	697286.5
58	560608.0
59	461112.0
60	395782.5
61	341282.5
62	285567.0
63	234866.0
64	200756.5
65	171798.0
66	143813.0
67	118458.5
68	91490.5
69	68685.5
70	55544.5
71	43211.0
72	28155.0
73	17418.0
74	9540.5
75	4553.5
76	2540.5
77	1545.0
78	1122.5
79	887.0
80	695.5
81	563.5
82	503.0
83	446.0
84	390.0
85	334.5
86	282.0
87	243.5
88	222.5
89	190.5
90	153.5
91	131.5
92	112.5
93	103.5
94	94.0
95	75.0
96	66.5
97	70.0
98	88.5
99	153.0
100	699.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.017711611659583956
2	0.00687747144979051
3	6.651052636628683E-4
4	0.0015000246371971071
5	2.603816351361016E-4
6	0.001157566182289843
7	0.0
8	0.02416737806986056
9	0.0
10-11	0.0
12-13	0.0037486464754105065
14-15	0.0
16-17	0.0010273753647217921
18-19	0.0
20-21	3.6651545380570827E-4
22-23	7.075587911307109E-6
24-25	2.5472116480705594E-5
26-27	0.005401503811491847
28-29	9.736008965958582E-4
30-31	1.938711087698148E-4
32-33	4.981213889560205E-4
34-35	0.0
36-37	0.0
38-39	9.169961933054014E-4
40-41	0.0
42-43	6.226517361950256E-5
44-45	0.0019005029129770896
46-47	0.0
48-49	0.0015608746932343485
50-51	0.0012297371789851757
52-53	7.103890262952339E-4
54-55	8.080321394712719E-4
56-57	0.0084807996704927
58-59	6.353877944353784E-4
60-61	0.0
62-63	0.00484677771924537
64-65	7.075587911307109E-6
66-67	0.002695798994208009
68-69	3.198165735910813E-4
70-71	5.377446812593404E-5
72-73	2.0377693184564475E-4
74-75	5.985947372965815E-4
76-77	3.4387357248952553E-4
78-79	2.589665175538402E-4
80-81	0.0017618213899154701
82-83	0.005209047820304294
84-85	5.759528559803987E-4
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	6.368029120176398E-5
96-97	0.004693945020361136
98-99	0.0
100-101	4.853853307156677E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3.5332753E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.793168794522487
#Duplication Level	Percentage of deduplicated	Percentage of total
1	61.672302274668176	19.607579161653383
2	15.348302228589361	9.759423269257745
3	7.043941254213418	6.718476398217228
4	3.9264983756177827	4.993433025097381
5	2.582314307064435	4.104997732250497
6	1.7461077530229443	3.330857911516971
7	1.2569445427361472	2.7973575008794946
8	0.9356053676536216	2.3796687503098286
9	0.7164712183732764	2.050100134394288
>10	4.316524453936225	24.918279083249846
>50	0.2870869719413661	6.275034171684613
>100	0.1551593028047345	9.081482837672425
>500	0.008902955389849358	1.9199681520655036
>1k	0.0038015909640937086	1.974151847487968
>5k	2.8052268692521485E-5	0.054915440424718784
>10k+	9.350756230840495E-6	0.03427458383805667
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.1320940658091376E-5	0.0	0.0	2.830235164522844E-6	8.490705493568531E-6
2	2.264188131618275E-5	5.660470329045688E-6	0.0	5.660470329045688E-6	8.490705493568531E-6
3	4.8113997796888345E-5	5.660470329045688E-6	2.830235164522844E-6	5.660470329045688E-6	2.264188131618275E-5
4	1.301908175680508E-4	5.660470329045688E-6	1.9811646151659905E-5	8.490705493568531E-6	2.264188131618275E-5
5	1.500024637197107E-4	8.490705493568531E-6	3.3962821974274125E-5	8.490705493568531E-6	2.264188131618275E-5
6	1.6698387470684778E-4	1.1320940658091376E-5	6.50954087840254E-5	8.490705493568531E-6	2.264188131618275E-5
7	1.8396528569398485E-4	1.1320940658091376E-5	9.622799559377669E-5	8.490705493568531E-6	2.264188131618275E-5
8	2.0943740217469042E-4	1.1320940658091376E-5	1.1037917141639091E-4	1.4151175822614218E-5	2.264188131618275E-5
9	2.320792834908732E-4	1.1320940658091376E-5	1.1886987690995943E-4	1.9811646151659905E-5	2.264188131618275E-5
10-11	2.8726886919906865E-4	1.4151175822614218E-5	1.3726640547935792E-4	2.264188131618275E-5	2.264188131618275E-5
12-13	3.3679798457821844E-4	1.6981410987137063E-5	1.669838747068478E-4	2.264188131618275E-5	2.4056998898444172E-5
14-15	4.174596867671194E-4	1.9811646151659905E-5	1.938711087698148E-4	2.5472116480705594E-5	2.5472116480705594E-5
16-17	5.094423296141119E-4	2.122676373392133E-5	2.915142219458529E-4	2.5472116480705594E-5	2.5472116480705594E-5
18-19	6.537843230047769E-4	2.6887234062967015E-5	3.169863384265585E-4	2.6887234062967015E-5	2.5472116480705594E-5
20-21	8.08032139471272E-4	2.8302351645228436E-5	3.5377939556535546E-4	2.8302351645228436E-5	2.5472116480705594E-5
22-23	9.96242777912041E-4	3.820817472105839E-5	4.075538636912895E-4	2.8302351645228436E-5	2.5472116480705594E-5
24-25	0.0013245500569966909	5.094423296141118E-5	4.174596867671194E-4	2.8302351645228436E-5	2.5472116480705594E-5
26-27	0.0018835215019899526	6.651052636628683E-5	4.273655098429494E-4	2.8302351645228436E-5	2.5472116480705594E-5
28-29	0.0025854198227916176	6.792564394854825E-5	4.4151668566556364E-4	3.113258680975128E-5	2.5472116480705594E-5
30-31	0.0033156204952385112	8.349193735342389E-5	4.641585669817464E-4	3.254770439201271E-5	2.8302351645228436E-5
32-33	0.0042991272149102	8.773729010020816E-5	4.853853307156677E-4	3.3962821974274125E-5	2.8302351645228436E-5
34-35	0.005411409634567677	8.773729010020816E-5	6.056703252078886E-4	3.3962821974274125E-5	2.9717469227489858E-5
36-37	0.006941151740992274	9.198264284699242E-5	6.283122065240713E-4	3.3962821974274125E-5	3.3962821974274125E-5
38-39	0.009035525762739178	9.905823075829953E-5	6.50954087840254E-4	3.3962821974274125E-5	3.3962821974274125E-5
40-41	0.01211057626899325	1.0188846592282238E-4	7.245402021178479E-4	3.3962821974274125E-5	3.3962821974274125E-5
42-43	0.01635168366303073	1.0754893625186806E-4	7.811449054083049E-4	3.679305713879697E-5	4.386864505010408E-5
44-45	0.021093742681188753	1.1320940658091376E-4	8.037867867244875E-4	3.679305713879697E-5	4.669888021462693E-5
46-47	0.026198071800405703	1.2311522965674368E-4	8.646368427617288E-4	3.679305713879697E-5	4.8113997796888345E-5
48-49	0.032823652320553676	1.358512878970965E-4	9.000147823182643E-4	3.679305713879697E-5	4.952911537914976E-5
50-51	0.04224125983050345	1.3868152306161934E-4	9.198264284699243E-4	3.679305713879697E-5	5.094423296141119E-5
52-53	0.05467731314341682	1.4292687580840362E-4	9.736008965958582E-4	3.679305713879697E-5	5.518958570819545E-5
54-55	0.06885962155284078	1.4434199339066503E-4	0.0010386963053798837	3.679305713879697E-5	5.9434938454979715E-5
56-57	0.08432968696212265	1.5000246371971073E-4	0.0011193580075687846	3.679305713879697E-5	5.9434938454979715E-5
58-59	0.10291725640512643	1.5283269888423357E-4	0.0011660568877834116	3.820817472105839E-5	5.9434938454979715E-5
60-61	0.12622141274980753	1.5283269888423357E-4	0.0012254918262383915	4.103840988558123E-5	5.9434938454979715E-5
62-63	0.1559643540937781	1.54247816466495E-4	0.0013344558800725207	4.245352746784265E-5	5.9434938454979715E-5
64-65	0.1923484422513015	1.6132340437780208E-4	0.0013896454657807162	4.245352746784265E-5	6.368029120176398E-5
66-67	0.2354430179839086	1.7264434503589347E-4	0.0014009664064388075	4.245352746784265E-5	7.07558791130711E-5
68-69	0.2855395955135452	1.8538040327624626E-4	0.0014207780525904675	4.386864505010408E-5	7.358611427759394E-5
70-71	0.3457882265783252	1.9104087360529196E-4	0.0014250234053372517	5.094423296141119E-5	7.78314670243782E-5
72-73	0.4216102266358922	1.9245599118755338E-4	0.0014646466976405716	5.9434938454979715E-5	8.207681977116247E-5
74-75	0.5166665048715564	1.9528622635207622E-4	0.0014745525207164015	5.9434938454979715E-5	8.349193735342389E-5
76-77	0.6347184438189688	2.0377693184564475E-4	0.001478797873463186	5.9434938454979715E-5	8.632217251794674E-5
78-79	0.7782099515426947	2.0943740217469042E-4	0.0014872885789567543	5.9434938454979715E-5	8.773729010020816E-5
80-81	0.9485971840348811	2.1651299008599754E-4	0.0015056851075261528	6.085005603724114E-5	9.0567525264731E-5
82-83	1.1511656054652746	2.3490951865539603E-4	0.001519836283348767	6.226517361950256E-5	9.0567525264731E-5
84-85	1.3992526990466891	2.3632463623765744E-4	0.0015297421064245972	6.226517361950256E-5	9.481287801151527E-5
86-87	1.7058053755392342	2.4056998898444172E-4	0.0015622898108166097	6.226517361950256E-5	9.905823075829953E-5
88-89	2.0749656841061888	2.4340022414896456E-4	0.001579271221803747	6.226517361950256E-5	9.905823075829953E-5
90	2.3903288826658935	2.4340022414896456E-4	0.0015877619272973154	6.226517361950256E-5	1.0188846592282238E-4
>>END_MODULE
