##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	SRR19654109_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	34618062
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.665957325976247	32.0	32.0	32.0	32.0	32.0
2	31.701008970403947	32.0	32.0	32.0	32.0	32.0
3	35.83334399250888	37.0	37.0	37.0	32.0	37.0
4	36.39436066640588	37.0	37.0	37.0	37.0	37.0
5	36.56795285074017	37.0	37.0	37.0	37.0	37.0
6	40.196400797941834	41.0	41.0	41.0	37.0	41.0
7	40.225481166450045	41.0	41.0	41.0	37.0	41.0
8	40.26209271911293	41.0	41.0	41.0	41.0	41.0
9	40.30630931910631	41.0	41.0	41.0	41.0	41.0
10-11	40.33650572351509	41.0	41.0	41.0	41.0	41.0
12-13	40.31477344110135	41.0	41.0	41.0	41.0	41.0
14-15	40.30951436565109	41.0	41.0	41.0	41.0	41.0
16-17	40.30761252608537	41.0	41.0	41.0	41.0	41.0
18-19	40.30837656365628	41.0	41.0	41.0	41.0	41.0
20-21	40.27753916438188	41.0	41.0	41.0	41.0	41.0
22-23	40.2848710306198	41.0	41.0	41.0	41.0	41.0
24-25	40.27254177313566	41.0	41.0	41.0	41.0	41.0
26-27	40.19214277506349	41.0	41.0	41.0	41.0	41.0
28-29	40.20464796671749	41.0	41.0	41.0	41.0	41.0
30-31	40.11916728614098	41.0	41.0	41.0	41.0	41.0
32-33	40.144929314645054	41.0	41.0	41.0	41.0	41.0
34-35	40.15759544829517	41.0	41.0	41.0	41.0	41.0
36-37	40.14423717595746	41.0	41.0	41.0	41.0	41.0
38-39	40.07282074311381	41.0	41.0	41.0	37.0	41.0
40-41	40.09850867734883	41.0	41.0	41.0	37.0	41.0
42-43	40.08127856781815	41.0	41.0	41.0	37.0	41.0
44-45	40.079967200359164	41.0	41.0	41.0	37.0	41.0
46-47	40.06718214035205	41.0	41.0	41.0	37.0	41.0
48-49	39.97957813178566	41.0	41.0	41.0	37.0	41.0
50-51	39.93863771172401	41.0	41.0	41.0	37.0	41.0
52-53	39.98436703360228	41.0	41.0	41.0	37.0	41.0
54-55	39.95382956446262	41.0	41.0	41.0	37.0	41.0
56-57	39.94281732177844	41.0	41.0	41.0	37.0	41.0
58-59	39.94470476134684	41.0	41.0	41.0	37.0	41.0
60-61	39.92486792010483	41.0	41.0	41.0	37.0	41.0
62-63	39.88446744939101	41.0	41.0	41.0	37.0	41.0
64-65	39.83278429913263	41.0	41.0	41.0	37.0	41.0
66-67	39.81010337898176	41.0	41.0	41.0	37.0	41.0
68-69	39.79036170482334	41.0	41.0	41.0	37.0	41.0
70-71	39.76738196667392	41.0	41.0	41.0	37.0	41.0
72-73	39.73874132526541	41.0	41.0	41.0	37.0	41.0
74-75	39.721670178995	41.0	41.0	41.0	37.0	41.0
76-77	39.190262990458564	41.0	39.0	41.0	34.5	41.0
78-79	39.51732396227149	41.0	41.0	41.0	37.0	41.0
80-81	39.69849354940782	41.0	41.0	41.0	37.0	41.0
82-83	39.77107857742008	41.0	41.0	41.0	37.0	41.0
84-85	39.808980540274035	41.0	41.0	41.0	37.0	41.0
86-87	39.81827236602673	41.0	41.0	41.0	37.0	41.0
88-89	39.70210192586748	41.0	41.0	41.0	37.0	41.0
90-91	39.723501015163706	41.0	41.0	41.0	37.0	41.0
92-93	39.67753730696999	41.0	41.0	41.0	37.0	41.0
94-95	39.672692393930085	41.0	41.0	41.0	37.0	41.0
96-97	39.63910245755525	41.0	41.0	41.0	37.0	41.0
98-99	39.59719125813571	41.0	41.0	41.0	37.0	41.0
100-101	39.09017837278124	41.0	39.0	41.0	34.5	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	3.0
16	4.0
17	22.0
18	69.0
19	145.0
20	465.0
21	1375.0
22	3639.0
23	7625.0
24	14882.0
25	26885.0
26	44933.0
27	71025.0
28	104131.0
29	143192.0
30	187835.0
31	236777.0
32	289814.0
33	354230.0
34	424844.0
35	518871.0
36	656450.0
37	887129.0
38	1376667.0
39	3249173.0
40	2.6017877E7
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.22679354731262	11.456315669020995	8.223867502309556	43.09302328135683
2	22.702536034512853	14.024670127403436	34.860980952659915	28.4118128854238
3	19.79740274731317	18.246393549536783	24.34085452726802	37.61534917588202
4	22.539040458128476	26.26562688575692	23.01571358905071	28.179619067063893
5	23.495229166785826	30.970326992885965	24.332358639833735	21.20208520049447
6	20.173928280560595	33.16507145893956	24.69694866223303	21.964051598266824
7	15.815937356631924	23.734572432159837	41.317988857955136	19.131501353253107
8	19.248821612255476	24.146698333372907	30.087085753096172	26.517394301275445
9	18.604490338020653	22.60776180942769	33.44753672230409	25.34021113024756
10-11	21.949653811785208	30.666553931070407	23.803277174097982	23.580515083046404
12-13	21.72577569478037	24.497330324268297	27.42372464408897	26.353169336862358
14-15	20.840346290904442	26.09529383822815	27.79867053216324	25.265689338704174
16-17	21.579510027521568	26.455020234374	27.019274052600505	24.946195685503927
18-19	21.397998913521516	26.850950395689654	26.716146583687262	25.034904107101575
20-21	21.55452405163524	26.72498391157772	26.85568158032648	24.86481045646056
22-23	21.52439527088489	26.944327501637726	26.801968290425965	24.729308937051417
24-25	21.516594116307566	26.631795235678247	26.59436819947806	25.257242448536125
26-27	21.316815770911727	26.787351643197127	26.82270168676687	25.07313089912428
28-29	21.42736066164954	26.947995844007465	26.712892473976808	24.91175102036619
30-31	21.5530442575116	26.69658570483424	26.659019361435426	25.091350676218738
32-33	21.596028396968034	26.605670473407784	26.762758989801334	25.035542139822848
34-35	21.6712781322074	26.766066511753316	26.544139010439117	25.018516345600165
36-37	21.546768918495594	26.634213086547696	26.48681748071935	25.332200514237357
38-39	21.604824692150967	26.717831087445987	26.619428822865075	25.057915397537972
40-41	21.744173919154893	26.961265580974253	26.57065445446736	24.723906045403496
42-43	21.71473377105859	26.565629237130604	26.59167200058744	25.12796499122337
44-45	21.729203096348954	26.51828689890266	26.7348125958062	25.017697408942187
46-47	21.750856916182705	26.61341545104015	26.597910198110338	25.03781743466681
48-49	21.62164797093494	26.539921558867157	26.506207944280646	25.332222525917253
50-51	21.676875848739062	26.48832686791923	26.714537403448485	25.12025987989322
52-53	21.98131310437401	26.634171046012895	26.431362645947093	24.953153203666005
54-55	21.801471728833345	26.534573194767518	26.46701886431424	25.196936212084893
56-57	21.75479024389032	26.393735679979187	26.795629668989555	25.05584440714094
58-59	21.861258828403614	26.4312483475912	26.679175565270953	25.028317258734234
60-61	21.82207599250302	26.32797612826603	26.58320844049679	25.266739438734163
62-63	21.781581674131854	26.27107341173761	26.752803392030767	25.19454152209977
64-65	21.97650335044315	26.486948268529193	26.599940706052088	24.93660767497557
66-67	21.833459013149344	26.323503500612205	26.577109091539086	25.265928394699355
68-69	21.88259500540175	26.271859710194477	26.779085161899946	25.066460122503827
70-71	22.078909241488724	26.54372923481873	26.57022142834549	24.807140095347055
72-73	22.015024988978297	26.367450898897804	26.44277573943914	25.174748372684757
74-75	21.93918163298685	26.33467321192041	26.629865935302792	25.096279219789945
76-77	21.936017934631824	26.519738816394778	26.52751084838308	25.016732400590314
78-79	21.923079084020923	26.31511588249966	26.48203019686703	25.279774836612383
80-81	22.00149707788163	26.368797517147247	26.61057306740281	25.019132337568312
82-83	22.10748111725167	26.576783021062656	26.41991936857285	24.895816493112825
84-85	22.15271690315117	26.434430716411732	26.33184696054839	25.081005419888708
86-87	22.14701306996736	26.327063069846034	26.558885337332217	24.967038522854388
88-89	22.335159894277155	26.42435616413189	26.438660835491024	24.80182310609993
90-91	22.24748043556492	26.280229338806123	26.363590734904047	25.108699490724916
92-93	22.25097328584331	26.294987645971936	26.3376681780871	25.116370890097663
94-95	22.365535655924354	26.48836899073091	26.227360734405064	24.918734618939673
96-97	22.340177536174217	26.509741663279456	25.996944245037668	25.15313655550866
98-99	22.443068161782747	26.541317590726454	26.22774196969167	24.787872277799128
100-101	22.52433281793764	26.668513390495402	26.054124578088743	24.75302921347821
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	100.0
1	81.0
2	48.0
3	43.0
4	56.0
5	61.0
6	81.0
7	103.0
8	116.5
9	129.0
10	146.5
11	166.5
12	193.5
13	238.0
14	271.0
15	324.0
16	391.5
17	487.5
18	645.0
19	896.5
20	1210.5
21	1620.5
22	2413.0
23	3725.0
24	5906.0
25	8682.5
26	12408.0
27	18319.5
28	26416.0
29	38006.0
30	54264.0
31	74567.5
32	103945.0
33	142719.0
34	190579.0
35	256819.5
36	345101.0
37	462995.5
38	621138.5
39	831222.0
40	1075498.0
41	1331125.0
42	1601752.5
43	1864586.0
44	2073371.5
45	2198243.0
46	2264672.0
47	2275602.0
48	2195545.0
49	2047727.0
50	1867145.5
51	1661128.5
52	1482444.5
53	1325956.5
54	1141646.0
55	953045.5
56	789296.0
57	642798.5
58	513459.0
59	408091.5
60	327333.5
61	275978.0
62	233688.0
63	190561.0
64	158152.5
65	134537.0
66	111647.0
67	86606.0
68	57635.5
69	36894.0
70	26524.0
71	19224.5
72	14461.5
73	11331.0
74	7440.5
75	3638.0
76	1805.5
77	694.5
78	102.5
79	36.0
80	16.5
81	10.0
82	4.0
83	1.5
84	3.0
85	2.5
86	0.5
87	1.0
88	1.0
89	0.5
90	1.5
91	2.5
92	1.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.5
98	0.5
99	0.0
100	2.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.059240751258692645
2	0.0
3	6.0661974665132905E-5
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	5.430691065259517E-4
12-13	0.0
14-15	0.0
16-17	0.0014010027482185454
18-19	0.002072617467725374
20-21	0.0
22-23	0.0
24-25	1.1265795294953253E-4
26-27	0.0
28-29	2.8308921510395357E-4
30-31	0.001845857229096187
32-33	0.0
34-35	0.0
36-37	4.332998190366636E-6
38-39	0.002809227160087702
40-41	1.4443327301222118E-6
42-43	0.0
44-45	0.0
46-47	0.0021997187479761288
48-49	0.0
50-51	0.0015367700248500335
52-53	4.8096279913069655E-4
54-55	0.0
56-57	6.54282726745362E-4
58-59	1.1410228567965474E-4
60-61	0.0011092475367338588
62-63	0.0033479632684232873
64-65	0.0020249544876313414
66-67	9.575926000710265E-4
68-69	3.538615188799419E-4
70-71	9.157069508974824E-4
72-73	0.0
74-75	0.0
76-77	9.980339165144485E-4
78-79	1.4443327301222118E-6
80-81	0.0018039715799226428
82-83	0.0012089064951122915
84-85	2.310932368195539E-5
86-87	0.0010919155439723922
88-89	0.0
90-91	0.002078394798645863
92-93	0.001171353844129114
94-95	0.0
96-97	2.9753254240517566E-4
98-99	0.001200240498731558
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3.4618062E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.18872707468652
#Duplication Level	Percentage of deduplicated	Percentage of total
1	60.05376952120175	18.12946857881687
2	16.496460370146025	9.960142796254411
3	7.2460898344620075	6.5625068511370195
4	3.9681388636335795	4.7917224459476335
5	2.524225401554009	3.8101575861252472
6	1.79622282409767	3.2535408361204325
7	1.2324532424558223	2.604433619816789
8	0.9471472551135774	2.287453598732981
9	0.7453328240515091	2.025058428458672
>10	4.4762758891406405	24.89251061307912
>50	0.3224010806218957	6.671179839726007
>100	0.17411236207795583	9.723459810003188
>500	0.011987240009591633	2.479622826742262
>1k	0.005352683316384205	2.7512619585340112
>5k	3.0608117399235504E-5	0.05748021050550335
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.5997989142199814E-5	0.0	0.0	0.0	0.0
2	4.0441316443421937E-5	0.0	0.0	0.0	2.8886654602444237E-6
3	6.06619746651329E-5	0.0	0.0	0.0	8.665996380733272E-6
4	1.1554661840977695E-4	0.0	0.0	0.0	1.1554661840977695E-5
5	1.328786111712435E-4	0.0	0.0	0.0	1.444332730122212E-5
6	1.6465393123393215E-4	2.8886654602444237E-6	2.8886654602444237E-6	0.0	1.444332730122212E-5
7	1.8776325491588755E-4	5.777330920488847E-6	2.8886654602444237E-6	0.0	1.7331992761466544E-5
8	2.166499095183318E-4	8.665996380733272E-6	2.8886654602444237E-6	0.0	1.7331992761466544E-5
9	2.2820457135930947E-4	8.665996380733272E-6	2.8886654602444237E-6	0.0	3.177532006268866E-5
10-11	2.744232187232203E-4	2.5997989142199814E-5	2.8886654602444237E-6	0.0	3.466398552293309E-5
12-13	3.264191970076199E-4	3.466398552293309E-5	2.8886654602444237E-6	0.0	3.466398552293309E-5
14-15	4.56409142718619E-4	3.466398552293309E-5	2.8886654602444237E-6	0.0	3.466398552293309E-5
16-17	5.416247737958295E-4	3.61083182530553E-5	2.8886654602444237E-6	0.0	3.466398552293309E-5
18-19	6.383950667140177E-4	3.755265098317751E-5	2.8886654602444237E-6	0.0	3.466398552293309E-5
20-21	7.308323614418392E-4	3.755265098317751E-5	8.665996380733272E-6	0.0	3.755265098317751E-5
22-23	8.348243180106385E-4	4.0441316443421937E-5	8.665996380733272E-6	0.0	3.755265098317751E-5
24-25	9.489266036902933E-4	5.055164555427742E-5	8.665996380733272E-6	0.0	3.755265098317751E-5
26-27	0.0010745835512109255	8.232696561696608E-5	1.444332730122212E-5	0.0	3.755265098317751E-5
28-29	0.0012666798043171799	8.37712983470883E-5	2.310932368195539E-5	0.0	3.899698371329972E-5
30-31	0.0016075423286260218	8.521563107721051E-5	2.310932368195539E-5	0.0	4.0441316443421937E-5
32-33	0.001984513171187919	9.67702929181882E-5	2.310932368195539E-5	0.0	4.0441316443421937E-5
34-35	0.0024755862994294713	1.0399195656879926E-4	2.310932368195539E-5	0.0	4.0441316443421937E-5
36-37	0.0031804206717291105	1.0399195656879926E-4	2.310932368195539E-5	0.0	4.0441316443421937E-5
38-39	0.004204452577385759	1.0976928748928811E-4	2.310932368195539E-5	0.0	4.0441316443421937E-5
40-41	0.005738333936775548	1.1843528387002138E-4	2.310932368195539E-5	0.0	4.332998190366636E-5
42-43	0.007823950399072022	1.4010027482185455E-4	2.310932368195539E-5	0.0	4.332998190366636E-5
44-45	0.010324090354913572	1.6176526577368775E-4	2.310932368195539E-5	0.0	4.332998190366636E-5
46-47	0.013110208191319316	1.7909725853515427E-4	2.310932368195539E-5	0.0	4.332998190366636E-5
48-49	0.01667048837107057	1.7909725853515427E-4	2.310932368195539E-5	0.0	4.332998190366636E-5
50-51	0.021991410148840797	1.8776325491588755E-4	2.310932368195539E-5	0.0	4.477431463378857E-5
52-53	0.02888087727152375	1.9787358402674303E-4	2.7442321872322025E-5	0.0	4.7662980094032996E-5
54-55	0.03669182867602467	2.036509149472319E-4	3.321965279281088E-5	0.0	5.921764193501069E-5
56-57	0.04550225832977016	2.310932368195539E-4	3.899698371329972E-5	0.0	6.06619746651329E-5
58-59	0.05594045097036339	2.3687056774004275E-4	4.0441316443421937E-5	0.0	6.06619746651329E-5
60-61	0.06952006729897242	2.469808968508983E-4	4.0441316443421937E-5	0.0	6.06619746651329E-5
62-63	0.0873416888559504	2.4842522958102046E-4	4.0441316443421937E-5	0.0	6.06619746651329E-5
64-65	0.10946886628142269	2.4842522958102046E-4	4.0441316443421937E-5	0.0	6.210630739525512E-5
66-67	0.1352588715104849	2.4842522958102046E-4	4.0441316443421937E-5	0.0	6.643930558562175E-5
68-69	0.16561441249946343	2.4842522958102046E-4	4.0441316443421937E-5	5.777330920488847E-6	6.643930558562175E-5
70-71	0.20314250982622886	2.4842522958102046E-4	4.0441316443421937E-5	5.777330920488847E-6	6.788363831574396E-5
72-73	0.2507433836128666	2.4842522958102046E-4	4.332998190366636E-5	5.777330920488847E-6	7.077230377598839E-5
74-75	0.3110702730846111	2.5709122596175374E-4	4.332998190366636E-5	5.777330920488847E-6	7.22166365061106E-5
76-77	0.38608458208896845	2.802005496437091E-4	4.332998190366636E-5	5.777330920488847E-6	7.36609692362328E-5
78-79	0.4778863704155363	2.8308921510395357E-4	4.332998190366636E-5	5.777330920488847E-6	7.510530196635502E-5
80-81	0.5892545342370696	2.85977880564198E-4	4.332998190366636E-5	5.777330920488847E-6	7.510530196635502E-5
82-83	0.7256486512734306	2.85977880564198E-4	4.332998190366636E-5	5.777330920488847E-6	7.799396742659944E-5
84-85	0.8961044670842637	2.9175521148468685E-4	4.477431463378857E-5	5.777330920488847E-6	7.799396742659944E-5
86-87	1.1102585696449443	3.0619853878590894E-4	4.621864736391078E-5	5.777330920488847E-6	7.799396742659944E-5
88-89	1.3683333861959113	3.1919753335700885E-4	4.910731282415521E-5	5.777330920488847E-6	8.37712983470883E-5
90	1.5902478885155384	3.264191970076199E-4	5.199597828439963E-5	5.777330920488847E-6	8.665996380733272E-5
>>END_MODULE
