##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	SRR21290293_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	95191489
Sequences flagged as poor quality	0
Sequence length	35-101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.038378326028706	37.0	37.0	37.0	37.0	37.0
2	36.083709584582714	37.0	37.0	37.0	37.0	37.0
3	36.182118697607514	37.0	37.0	37.0	37.0	37.0
4	36.160722814200334	37.0	37.0	37.0	37.0	37.0
5	36.119559197146295	37.0	37.0	37.0	37.0	37.0
6	36.039562717629096	37.0	37.0	37.0	37.0	37.0
7	35.99487572885849	37.0	37.0	37.0	37.0	37.0
8	36.06511192402926	37.0	37.0	37.0	37.0	37.0
9	36.01466177296586	37.0	37.0	37.0	37.0	37.0
10-11	36.043699368963544	37.0	37.0	37.0	37.0	37.0
12-13	36.020786968675324	37.0	37.0	37.0	37.0	37.0
14-15	36.01017349880934	37.0	37.0	37.0	37.0	37.0
16-17	35.9911754610751	37.0	37.0	37.0	37.0	37.0
18-19	35.9955143311184	37.0	37.0	37.0	37.0	37.0
20-21	35.98538749089218	37.0	37.0	37.0	37.0	37.0
22-23	35.95783390361716	37.0	37.0	37.0	37.0	37.0
24-25	35.94451514462601	37.0	37.0	37.0	37.0	37.0
26-27	35.913518560467104	37.0	37.0	37.0	37.0	37.0
28-29	35.89587172546487	37.0	37.0	37.0	37.0	37.0
30-31	35.87549644800703	37.0	37.0	37.0	37.0	37.0
32-33	35.85266410214468	37.0	37.0	37.0	37.0	37.0
34-35	35.834804301674495	37.0	37.0	37.0	37.0	37.0
36-37	35.82526036260433	37.0	37.0	37.0	37.0	37.0
38-39	35.8103446713632	37.0	37.0	37.0	37.0	37.0
40-41	35.81232939657811	37.0	37.0	37.0	37.0	37.0
42-43	35.77467941824408	37.0	37.0	37.0	37.0	37.0
44-45	35.76213797106483	37.0	37.0	37.0	37.0	37.0
46-47	35.76501017941137	37.0	37.0	37.0	37.0	37.0
48-49	35.74040044175025	37.0	37.0	37.0	37.0	37.0
50-51	35.71957992937736	37.0	37.0	37.0	37.0	37.0
52-53	35.70554048785786	37.0	37.0	37.0	37.0	37.0
54-55	35.699454324245956	37.0	37.0	37.0	37.0	37.0
56-57	35.666548569042234	37.0	37.0	37.0	37.0	37.0
58-59	35.68873641791596	37.0	37.0	37.0	37.0	37.0
60-61	35.65288527719518	37.0	37.0	37.0	37.0	37.0
62-63	35.641164455219055	37.0	37.0	37.0	37.0	37.0
64-65	35.62812933401632	37.0	37.0	37.0	37.0	37.0
66-67	35.60743533221479	37.0	37.0	37.0	37.0	37.0
68-69	35.57320263463057	37.0	37.0	37.0	37.0	37.0
70-71	35.577430687709715	37.0	37.0	37.0	37.0	37.0
72-73	35.55558379368132	37.0	37.0	37.0	37.0	37.0
74-75	35.568958483125414	37.0	37.0	37.0	37.0	37.0
76-77	35.54207907032012	37.0	37.0	37.0	37.0	37.0
78-79	35.49437098186353	37.0	37.0	37.0	37.0	37.0
80-81	35.48935419392731	37.0	37.0	37.0	37.0	37.0
82-83	35.45641728429055	37.0	37.0	37.0	37.0	37.0
84-85	35.411455517019334	37.0	37.0	37.0	37.0	37.0
86-87	35.37299818128939	37.0	37.0	37.0	37.0	37.0
88-89	35.40065948919549	37.0	37.0	37.0	37.0	37.0
90-91	35.34010573493114	37.0	37.0	37.0	37.0	37.0
92-93	35.38313972175027	37.0	37.0	37.0	37.0	37.0
94-95	35.33459456907475	37.0	37.0	37.0	37.0	37.0
96-97	35.30813997799246	37.0	37.0	37.0	37.0	37.0
98-99	35.30168224393131	37.0	37.0	37.0	37.0	37.0
100-101	35.284183350299216	37.0	37.0	37.0	37.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	22559.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	60.0
12	2566.0
13	19984.0
14	43448.0
15	48123.0
16	44188.0
17	44084.0
18	44112.0
19	44642.0
20	54171.0
21	81390.0
22	118848.0
23	162566.0
24	201762.0
25	245175.0
26	295889.0
27	379538.0
28	487090.0
29	653242.0
30	874341.0
31	1250169.0
32	1826953.0
33	3019591.0
34	5565324.0
35	1.4116373E7
36	5.5016664E7
37	1.0528637E7
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.788005483235057	16.101066570753435	10.987871457290673	48.123056488720835
2	21.683721688658938	19.30152265929836	33.96644090745271	25.048314744589995
3	23.347154401390817	20.18600197654184	23.64667345520621	32.82017016686113
4	24.819764179221977	25.684149022027004	20.8099982878766	28.68608851087442
5	26.090232498983696	29.642759841668482	23.01457029609487	21.25243736325295
6	21.530024954298508	33.83467637484752	23.08469843067973	21.550600240174248
7	19.006006572376847	21.936405519117013	38.215973641562535	20.8416142669436
8	19.655475894241654	23.914064301167283	30.009273359131207	26.42118644545986
9	20.712192954406294	22.22200506451013	31.975162103328515	25.09063987775506
10-11	22.73770265406797	30.793640451135246	23.9181288540628	22.550528040733983
12-13	21.974467049277276	26.026698646579487	27.547109097024247	24.451725207118987
14-15	22.088087264590467	26.606173557067255	26.99421782284667	24.311521355495604
16-17	22.892158989012877	26.59003954853124	26.276614790479876	24.241186671976006
18-19	22.780647638618646	26.91719759619064	25.922602320928256	24.379552444262455
20-21	22.850452824778834	26.76860303976167	26.316073262838962	24.064870872620535
22-23	22.717567080677853	26.931810420935665	26.326088078463076	24.024534419923405
24-25	22.595113876557622	26.879988467249643	26.397191150272263	24.127706505920465
26-27	22.663126158332826	27.03443698823361	26.40565434710031	23.896782506333253
28-29	22.539293725049365	26.949500939342002	26.264134758317947	24.24707057729069
30-31	22.603039170493123	26.679054377889415	26.370710741502933	24.347195710114526
32-33	22.804000443163716	26.875420736893403	26.235901447094523	24.08467737284836
34-35	22.801055837667583	26.874008686157048	26.084709911915947	24.240225564259422
36-37	22.631910612010405	26.740666360872034	26.279162633608504	24.34826039350905
38-39	22.818493882219222	26.87855320376549	26.326871756632947	23.976081157382335
40-41	22.938031401627683	26.753065425252174	26.123784929765677	24.185118243354466
42-43	22.773091320828264	26.62787046991691	26.384274998573776	24.214763210681053
44-45	22.86972977087639	26.630543590598972	26.426927851357785	24.072798787166853
46-47	22.90884333721349	26.633817257842864	26.268124834129946	24.189214570813704
48-49	22.752034600248297	26.524453380877098	26.338754657846707	24.384757361027894
50-51	22.895682582385295	26.59538708543628	26.26981630556408	24.239114026614345
52-53	23.162615759749848	26.732658306972723	25.952837864983913	24.151888068293516
54-55	22.824421699989774	26.63633890900496	26.22557506745981	24.313664323545453
56-57	22.858408611483195	26.716206162259887	26.300703592816415	24.1246816334405
58-59	22.991167434322225	26.60700237580204	26.19848520840309	24.203344981472643
60-61	23.024471529331127	26.43618161500813	26.155982323750386	24.383364531910363
62-63	23.10524614959906	26.35374710403888	26.289981293862546	24.25102545249951
64-65	23.14791667917265	26.560796333609925	26.017216845984155	24.274070141233274
66-67	23.174116947780682	26.499608936797532	26.076240684668146	24.250033430753643
68-69	23.120751590969036	26.596798528828003	26.185699214257063	24.0967506659459
70-71	23.37492999156679	26.57432150046794	25.97094381195788	24.079804696007386
72-73	23.144178487740525	26.52780727445248	26.034100493608474	24.293913744198523
74-75	23.12377234860209	26.59540268984643	26.08941411259028	24.191410848961198
76-77	23.26755360484833	26.590419942122157	25.920059794502087	24.22196665852743
78-79	23.074902764783936	26.50684182544003	26.046166655241798	24.372088754534236
80-81	23.230840512928793	26.592956689587723	26.01106030851773	24.165142488965756
82-83	23.33054195651783	26.589594099177344	25.823899597930268	24.255964346374565
84-85	23.293465094103258	26.536288196873194	25.889596475210386	24.280650233813166
86-87	23.25553013660323	26.547160359955253	25.91787748498287	24.279432018458646
88-89	23.47049973342624	26.36037520097419	25.845782303135216	24.323342762464346
90-91	23.333854456331622	26.34886357705348	26.03906133655206	24.278220630062844
92-93	23.468477010925767	26.40230772993363	26.044067778404855	24.085147480735746
94-95	23.475980040854683	26.40663653949804	25.95220963599611	24.16517378365117
96-97	23.33566765575822	26.244695995563056	26.05049683726216	24.369139511416567
98-99	23.50291408195299	25.502865986487596	26.475069674781672	24.519150256777746
100-101	24.410600251095026	12.027908260854188	32.57828378724266	30.98320770080812
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	22723.0
1	11518.0
2	609.5
3	1331.5
4	2180.0
5	3073.0
6	3898.0
7	4611.0
8	5373.0
9	6186.0
10	6918.5
11	7514.5
12	8104.0
13	8895.0
14	9604.5
15	10151.5
16	11104.0
17	12361.0
18	13725.0
19	15493.5
20	17474.5
21	20417.0
22	24875.5
23	30777.0
24	38730.5
25	49266.5
26	63685.0
27	85923.0
28	115888.0
29	152325.0
30	204414.5
31	275573.5
32	376972.0
33	511644.0
34	681029.0
35	906174.5
36	1204588.5
37	1580526.0
38	2043725.5
39	2588070.5
40	3115517.0
41	3643697.5
42	4233728.5
43	4756202.5
44	5179124.5
45	5481316.5
46	5622360.5
47	5646575.0
48	5470818.0
49	5133860.5
50	4755947.0
51	4348503.0
52	3903558.0
53	3396885.0
54	2900615.0
55	2526089.0
56	2206018.5
57	1863289.5
58	1535861.0
59	1258530.5
60	1045815.5
61	904298.0
62	818633.0
63	731455.0
64	640232.0
65	562264.5
66	498000.5
67	432601.0
68	352844.0
69	276425.0
70	217946.0
71	170358.0
72	130895.0
73	104744.5
74	90886.5
75	69315.5
76	43331.0
77	33914.5
78	26570.5
79	21189.5
80	17771.5
81	12252.5
82	6884.5
83	3697.0
84	3142.0
85	2848.0
86	2558.5
87	2228.0
88	2034.0
89	1904.5
90	1789.0
91	1683.0
92	1561.5
93	1468.0
94	1446.0
95	1462.0
96	1529.0
97	1655.0
98	1958.0
99	2800.0
100	15060.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.06998839990831533
2	0.03919152898217613
3	0.024427604026658306
4	0.02428263308287992
5	0.024238511491295196
6	0.025125145379331127
7	0.023710102906363825
8	0.024340411357574206
9	0.02370064827959567
10-11	0.023741618328924345
12-13	0.023723234332430707
14-15	0.023719032276089305
16-17	0.024532655435193368
18-19	0.023770507466271486
20-21	0.023755800269076578
22-23	0.025774888341120496
24-25	0.02729760850783624
26-27	0.02974845786895927
28-29	0.03200023481090836
30-31	0.034035080594232534
32-33	0.0365384556596231
34-35	0.03836529965404785
36-37	2.0755961872863932E-4
38-39	5.391405645388144E-4
40-41	6.678977003951117E-4
42-43	0.0010993479017179612
44-45	8.860159829400618E-4
46-47	6.237988882474382E-4
48-49	5.812445348364366E-4
50-51	0.0010263982805616238
52-53	3.636887860399426E-4
54-55	3.2585653242199185E-5
56-57	9.723393781675235E-5
58-59	1.6819276459219798E-5
60-61	5.1510384606544156E-5
62-63	3.6794132105452175E-6
64-65	2.0500170842641703E-5
66-67	7.884921028415742E-6
68-69	1.8398688251327846E-5
70-71	2.733599926264296E-5
72-73	0.0
74-75	6.834450498745339E-6
76-77	3.6276422872368744E-5
78-79	5.520527980772411E-5
80-81	2.78666343680106E-4
82-83	0.0015411401527314584
84-85	1.793074784464129E-4
86-87	0.001251001925549105
88-89	5.421791931892953E-4
90-91	0.0022340856893874094
92-93	0.0010067738892781532
94-95	0.001100011116898576
96-97	0.0018175309906940623
98-99	0.0017090909048034437
100-101	3.5037583636817767E-4
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
34-35	37689.0
36-37	1848.0
38-39	2138.0
40-41	1956.0
42-43	2317.0
44-45	2213.0
46-47	2128.0
48-49	2119.0
50-51	2189.0
52-53	2395.0
54-55	2492.0
56-57	2485.0
58-59	2401.0
60-61	2601.0
62-63	2665.0
64-65	2849.0
66-67	2875.0
68-69	2728.0
70-71	2971.0
72-73	3278.0
74-75	3255.0
76-77	3405.0
78-79	3744.0
80-81	3864.0
82-83	3823.0
84-85	4147.0
86-87	4410.0
88-89	5478.0
90-91	15854.0
92-93	10318.0
94-95	33821.0
96-97	681710.0
98-99	8555322.0
100-101	8.5778001E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.094004571701443
#Duplication Level	Percentage of deduplicated	Percentage of total
1	59.96669934234621	15.048046274466817
2	15.043547946501393	7.5500572188823165
3	7.5330486897701014	5.6710307477982145
4	4.2822805311411685	4.298382689030583
5	2.7661772875119164	3.4707232749480363
6	1.850468845782944	2.786140420552098
7	1.332597183177551	2.340813986482575
8	1.0086683300496637	2.0249222148477375
9	0.7791841864438483	1.7597566383137462
>10	4.76469148801911	22.292671335763036
>50	0.37993889853268914	6.598081963654351
>100	0.25094214322080777	12.286580182748448
>500	0.024805860687101205	4.280827018829406
>1k	0.016010321017296537	7.535892755406031
>5k	7.006346820831429E-4	1.1278332890202485
>10k+	2.3831111635481053E-4	0.9282399892560869
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	8.404112682805077E-6	0.0	0.0	1.0505140853506347E-6	3.151542256051904E-6
2	8.404112682805077E-6	2.1010281707012694E-6	1.0505140853506347E-6	1.0505140853506347E-6	7.353598597454443E-6
3	1.3656683109558251E-5	2.1010281707012694E-6	1.0505140853506347E-6	1.0505140853506347E-6	1.3656683109558251E-5
4	1.785873945096079E-5	3.151542256051904E-6	4.202056341402539E-6	1.0505140853506347E-6	1.785873945096079E-5
5	2.6262852133765866E-5	3.151542256051904E-6	7.353598597454443E-6	1.0505140853506347E-6	1.995976762166206E-5
6	3.571747890192158E-5	3.151542256051904E-6	2.521233804841523E-5	1.0505140853506347E-6	1.995976762166206E-5
7	3.676799298727221E-5	3.151542256051904E-6	3.4666964816570946E-5	1.0505140853506347E-6	1.995976762166206E-5
8	4.307107749937602E-5	3.151542256051904E-6	3.4666964816570946E-5	2.1010281707012694E-6	2.1010281707012695E-5
9	4.6222619755427925E-5	3.151542256051904E-6	3.571747890192158E-5	2.1010281707012694E-6	2.3111309877713963E-5
10-11	5.305096131020705E-5	5.252570426753174E-6	4.1495306371350064E-5	2.1010281707012694E-6	2.521233804841523E-5
12-13	6.565713033441466E-5	8.404112682805077E-6	5.5151989480908316E-5	2.1010281707012694E-6	2.9939651432493085E-5
14-15	8.141484161467418E-5	8.929369725480394E-6	6.775815850511594E-5	3.151542256051904E-6	3.676799298727221E-5
16-17	9.97988381083103E-5	9.454626768155712E-6	1.0137460923633625E-4	5.252570426753174E-6	4.569736271275261E-5
18-19	1.1398077826054386E-4	1.0505140853506347E-5	1.1503129234589449E-4	5.252570426753174E-6	4.832364792612919E-5
20-21	1.2238489094334895E-4	1.1555654938856981E-5	1.244859191140502E-4	9.454626768155712E-6	5.935404582231086E-5
22-23	1.271122043274268E-4	1.1555654938856981E-5	1.397183733516344E-4	1.0505140853506347E-5	6.092981695033681E-5
24-25	1.486477430771148E-4	1.2080911981532298E-5	1.449709437783876E-4	1.0505140853506347E-5	6.250558807836275E-5
26-27	1.906683064911402E-4	1.8383996493636105E-5	1.5495082758921862E-4	1.1555654938856981E-5	7.038444371849253E-5
28-29	2.4739606710007445E-4	1.995976762166206E-5	1.665064825280756E-4	1.1555654938856981E-5	7.773804231594696E-5
30-31	3.072753699650606E-4	2.1535538749688012E-5	1.7386008112553003E-4	1.575771128025952E-5	7.87885564012976E-5
32-33	3.634778735313196E-4	2.2060795792363327E-5	1.8068842268030916E-4	1.575771128025952E-5	8.088958457199887E-5
34-35	4.070742080733709E-4	2.2060795792363327E-5	2.153553874968801E-4	1.6808225365610155E-5	8.14148416146742E-5
36-37	4.580241412128767E-4	2.2060795792363327E-5	2.3111309877713962E-4	1.6808225365610155E-5	9.297049655353118E-5
38-39	5.383884687422002E-4	2.2060795792363327E-5	2.4844658118542513E-4	1.7333482408285473E-5	1.034756374070375E-4
40-41	6.087729124606928E-4	2.2060795792363327E-5	2.6315377838033395E-4	1.785873945096079E-5	1.1082923600449196E-4
42-43	6.812583843498866E-4	2.3111309877713963E-5	2.8521457417269734E-4	1.8383996493636105E-5	1.1345552121786854E-4
44-45	7.568953984951323E-4	2.521233804841523E-5	2.9046714459945045E-4	1.8909253536311423E-5	1.244859191140502E-4
46-47	8.551184654754167E-4	2.6262852133765866E-5	3.0832588405041125E-4	1.995976762166206E-5	1.302637465834787E-4
48-49	9.570183317544282E-4	2.6262852133765866E-5	3.2881090871474865E-4	2.0485024664337377E-5	1.42344658565011E-4
50-51	0.0010442110008385308	2.941439438981777E-5	3.2986142280009927E-4	2.3111309877713963E-5	1.5127402829049138E-4
52-53	0.001148737152330919	2.941439438981777E-5	3.503464474644367E-4	2.363656692038928E-5	1.5600134167456924E-4
54-55	0.0012511622756526059	2.941439438981777E-5	3.734577573421506E-4	2.8889137347142452E-5	1.6598122548540026E-4
56-57	0.0013535873989742927	3.099016551784372E-5	3.9972060947591646E-4	2.941439438981777E-5	1.7648636633890664E-4
58-59	0.0014722954906189144	3.309119368854499E-5	4.0970049328674753E-4	2.941439438981777E-5	1.8383996493636105E-4
60-61	0.00159730666677564	3.676799298727221E-5	4.4646848627401974E-4	3.309119368854499E-5	1.9592087691789337E-4
62-63	0.0017207420718043395	3.729325002994753E-5	4.942668771574735E-4	3.414170777389563E-5	2.0379973255802314E-4
64-65	0.001853632103601195	3.7818507072622846E-5	5.168529299925122E-4	3.4666964816570946E-5	2.08527045942101E-4
66-67	0.0020054313889343615	3.7818507072622846E-5	5.215802433765902E-4	3.7293250029947535E-5	2.1325435932617883E-4
68-69	0.002146200276371347	3.7818507072622846E-5	5.315601271874211E-4	3.886902115797348E-5	2.1693115862490606E-4
70-71	0.0022696356814000465	3.8343764115298164E-5	5.341864124007977E-4	3.886902115797348E-5	2.3111309877713962E-4
72-73	0.0024030509702395765	3.886902115797348E-5	5.530956659371091E-4	3.886902115797348E-5	2.4687081005739914E-4
74-75	0.002572183737981029	3.991953524332412E-5	5.588734934065376E-4	3.886902115797348E-5	2.5580017978287954E-4
76-77	0.0027171546817594166	4.4121591584726654E-5	5.630755497479402E-4	3.991953524332412E-5	2.6578006359371056E-4
78-79	0.0028132767205689994	4.517210567007729E-5	5.667523490466674E-4	4.25458204567007E-5	2.7523469036186627E-4
80-81	0.002906772474165206	4.832364792612919E-5	5.69378634260044E-4	4.517210567007729E-5	2.878408593860739E-4
82-83	0.0029766316608410234	4.9374162011479826E-5	5.741059476441219E-4	4.6222619755427925E-5	2.9256817277015175E-4
84-85	0.0030801072982480607	4.9899419054155144E-5	5.756817187721478E-4	4.727313384077856E-5	2.978207431969049E-4
86-87	0.0031268551750461638	5.14751901821811E-5	5.772574899001737E-4	4.83236479261292E-5	3.0254805658098277E-4
88-89	0.003134734030686294	5.357621835288237E-5	5.804090321562256E-4	5.094993313950578E-5	3.156794826478657E-4
90	0.003134734030686294	5.357621835288237E-5	5.861868596256541E-4	5.14751901821811E-5	3.1935628194659294E-4
>>END_MODULE
