##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	SRR21290304_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	72267237
Sequences flagged as poor quality	0
Sequence length	35-101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.12499510670375	37.0	37.0	37.0	37.0	37.0
2	36.21757721275549	37.0	37.0	37.0	37.0	37.0
3	36.3326286709979	37.0	37.0	37.0	37.0	37.0
4	36.36210666252537	37.0	37.0	37.0	37.0	37.0
5	36.37821052989752	37.0	37.0	37.0	37.0	37.0
6	36.343650664269894	37.0	37.0	37.0	37.0	37.0
7	36.309843117428166	37.0	37.0	37.0	37.0	37.0
8	36.36941545170739	37.0	37.0	37.0	37.0	37.0
9	36.3515558371216	37.0	37.0	37.0	37.0	37.0
10-11	36.37294763324077	37.0	37.0	37.0	37.0	37.0
12-13	36.35310178663673	37.0	37.0	37.0	37.0	37.0
14-15	36.34898362033684	37.0	37.0	37.0	37.0	37.0
16-17	36.348823727687275	37.0	37.0	37.0	37.0	37.0
18-19	36.346966765866526	37.0	37.0	37.0	37.0	37.0
20-21	36.34041284849454	37.0	37.0	37.0	37.0	37.0
22-23	36.32773149331834	37.0	37.0	37.0	37.0	37.0
24-25	36.2979564653897	37.0	37.0	37.0	37.0	37.0
26-27	36.25653335826303	37.0	37.0	37.0	37.0	37.0
28-29	36.24352399137662	37.0	37.0	37.0	37.0	37.0
30-31	36.23556465843575	37.0	37.0	37.0	37.0	37.0
32-33	36.23231411905232	37.0	37.0	37.0	37.0	37.0
34-35	36.21930776874727	37.0	37.0	37.0	37.0	37.0
36-37	36.22038806498368	37.0	37.0	37.0	37.0	37.0
38-39	36.2159807058512	37.0	37.0	37.0	37.0	37.0
40-41	36.21700211206313	37.0	37.0	37.0	37.0	37.0
42-43	36.20048194168719	37.0	37.0	37.0	37.0	37.0
44-45	36.197220190613876	37.0	37.0	37.0	37.0	37.0
46-47	36.19624491949088	37.0	37.0	37.0	37.0	37.0
48-49	36.172542711658295	37.0	37.0	37.0	37.0	37.0
50-51	36.16717801033585	37.0	37.0	37.0	37.0	37.0
52-53	36.17031237629186	37.0	37.0	37.0	37.0	37.0
54-55	36.160609916178956	37.0	37.0	37.0	37.0	37.0
56-57	36.146315079150256	37.0	37.0	37.0	37.0	37.0
58-59	36.133242802081995	37.0	37.0	37.0	37.0	37.0
60-61	36.131461001377424	37.0	37.0	37.0	37.0	37.0
62-63	36.122283416458956	37.0	37.0	37.0	37.0	37.0
64-65	36.10804888679371	37.0	37.0	37.0	37.0	37.0
66-67	36.10784198282941	37.0	37.0	37.0	37.0	37.0
68-69	36.091019451890205	37.0	37.0	37.0	37.0	37.0
70-71	36.07512785873382	37.0	37.0	37.0	37.0	37.0
72-73	36.053078459142995	37.0	37.0	37.0	37.0	37.0
74-75	36.049737691111034	37.0	37.0	37.0	37.0	37.0
76-77	36.038051088117356	37.0	37.0	37.0	37.0	37.0
78-79	36.02446555975592	37.0	37.0	37.0	37.0	37.0
80-81	35.99898808736111	37.0	37.0	37.0	37.0	37.0
82-83	36.01199669969735	37.0	37.0	37.0	37.0	37.0
84-85	35.995804027242684	37.0	37.0	37.0	37.0	37.0
86-87	36.00815170628627	37.0	37.0	37.0	37.0	37.0
88-89	35.98400873777574	37.0	37.0	37.0	37.0	37.0
90-91	35.96548998856035	37.0	37.0	37.0	37.0	37.0
92-93	35.957291787451666	37.0	37.0	37.0	37.0	37.0
94-95	35.93156076697539	37.0	37.0	37.0	37.0	37.0
96-97	35.93417483265568	37.0	37.0	37.0	37.0	37.0
98-99	35.91568094281837	37.0	37.0	37.0	37.0	37.0
100-101	35.88716591429479	37.0	37.0	37.0	37.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	28513.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	8.0
15	67.0
16	307.0
17	890.0
18	1506.0
19	2304.0
20	3243.0
21	5192.0
22	9869.0
23	20137.0
24	39487.0
25	73266.0
26	121678.0
27	200898.0
28	308324.0
29	459471.0
30	628402.0
31	858976.0
32	1133092.0
33	1613048.0
34	2537300.0
35	5909645.0
36	4.3007246E7
37	1.5304368E7
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.543213771592445	15.965788681760811	11.600256481580713	46.89074106506603
2	21.49136764401508	19.29133206379796	33.67568562870728	25.541614663479677
3	23.016837790516046	20.10504053389373	24.0251389282902	32.852982747300025
4	24.617819235506406	25.669696925147704	21.053768088532905	28.65871575081298
5	25.766497651093097	29.515026763767704	23.19031923992882	21.528156345210384
6	21.032705394962402	34.02593600628937	23.420217112362064	21.521141486386167
7	18.521384735422515	21.77998465199911	38.74999093284095	20.948639679737422
8	19.659162307462687	23.918436045465032	29.79489504825694	26.627506598815337
9	20.706311488922637	22.00366415668782	31.968282440430183	25.32174191395936
10-11	22.679790827977524	30.549220387669084	24.191718834900794	22.579269949452595
12-13	21.96861910908359	25.70580114545931	27.834256309195453	24.49132343626165
14-15	22.04690309329852	26.272013372564967	27.256987523589693	24.42409601054682
16-17	22.93061872997248	26.3345647966787	26.635801454837555	24.099015018511263
18-19	22.6831999199652	26.795185766086988	26.08313407287496	24.43848024107286
20-21	22.644796662243323	26.5760060269081	26.41079817896499	24.368399131883592
22-23	22.518390613618937	26.71325877060122	26.599258267197744	24.169092348582094
24-25	22.5788752358425	26.43819824418827	26.702186658300814	24.280739861668415
26-27	22.722155782478044	26.69888491656328	26.523196642936863	24.055762658021816
28-29	22.56297596276259	26.468586803768527	26.618050504812217	24.350386728656662
30-31	22.60919537874784	26.15358874803025	26.73865882392291	24.498557049298995
32-33	22.802593285157016	26.41861300285723	26.55341975055982	24.225373961425927
34-35	22.758317212334973	26.532820690857882	26.404427022062183	24.304435074744962
36-37	22.48877488027576	26.44155503467514	26.587557442925036	24.482112642124065
38-39	22.90373755395875	26.54485628237715	26.638210331365098	23.913195832299007
40-41	23.00175704080032	26.479489701532895	26.202301690635522	24.316451567031265
42-43	22.754257844076314	26.55523557342676	26.375087529420238	24.31541905307669
44-45	22.923862323604542	26.411737926218343	26.613157592431016	24.051242157746103
46-47	22.818451049209663	26.154594184133	26.655898075881506	24.371056690775827
48-49	22.599132577292664	25.988664589074062	26.785349928126923	24.626852905506354
50-51	22.865246150231364	25.97715494146737	26.644604240622304	24.512994667678964
52-53	23.087221114601565	26.334824660017215	26.27275024124782	24.305203984133396
54-55	22.603151067587987	26.31333189657109	26.634516728745627	24.449000307095297
56-57	22.74677956777664	26.503800315405606	26.49616521309056	24.253254903727196
58-59	22.744532506117395	26.430351915071604	26.45302845051184	24.37208712829916
60-61	22.791354232968235	26.280015982754435	26.4396240484276	24.489005735849734
62-63	22.931815028363843	26.092594382510715	26.54152146404676	24.43406912507869
64-65	22.92204700332311	26.34796026045809	26.260179906278676	24.469812829940118
66-67	23.021838629060703	26.22672398711848	26.33083163940378	24.42060574441704
68-69	22.980183432420556	26.344478435978893	26.548431335538638	24.126906796061917
70-71	23.345140361586083	26.319147805590724	26.31385762469687	24.021854208126324
72-73	23.090159815095763	26.16356805561048	26.321710819107597	24.424561310186157
74-75	23.21141887161466	26.34185699491593	26.35062221833734	24.09610191513207
76-77	23.157758507170165	26.36175924308593	26.339114349421163	24.141367900322745
78-79	22.925797395961467	26.409013294063467	26.179675924715973	24.485513385259093
80-81	23.17837478504244	26.336121926904504	26.25043253285882	24.235070755194236
82-83	23.213270417491643	26.47339418621976	25.94781014057889	24.365525255709702
84-85	23.08042108588658	26.49867496555211	26.061649036766312	24.359254911795
86-87	23.04848843490896	26.45469422203357	26.139798348096143	24.357018994961326
88-89	23.182786513397975	26.203707078318793	26.107564717725563	24.505941690557673
90-91	23.09950364465232	26.204192695490047	26.41040717435461	24.285896485503024
92-93	23.449812830275825	26.096925620358697	26.328443520219302	24.124818029146176
94-95	23.26714336029202	26.180447205728914	26.368072009746573	24.18433742423249
96-97	23.30287749722054	25.972668856959437	26.390750392809164	24.33370325301086
98-99	23.46705278394983	25.15196073341834	26.84284912278988	24.538137359841954
100-101	24.328473832062844	11.715510029440127	32.772973090915286	31.183043047581744
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	28612.0
1	14332.5
2	56.0
3	66.0
4	75.0
5	79.5
6	86.5
7	99.0
8	112.5
9	123.5
10	154.0
11	184.5
12	234.5
13	308.0
14	414.0
15	584.0
16	852.0
17	1283.5
18	1925.0
19	2779.5
20	3641.5
21	5335.5
22	8879.5
23	13750.0
24	18703.5
25	25163.5
26	32625.0
27	45175.0
28	62061.0
29	81228.0
30	112356.5
31	151043.0
32	219268.0
33	319450.5
34	429780.0
35	559476.0
36	766441.5
37	1061437.0
38	1409318.5
39	1901514.0
40	2398968.5
41	2818337.5
42	3264886.0
43	3746487.0
44	4128373.0
45	4379563.5
46	4492237.5
47	4457674.5
48	4312338.5
49	4139384.0
50	3878444.5
51	3555336.0
52	3151353.5
53	2584919.5
54	2133886.0
55	1844102.5
56	1605561.0
57	1360941.0
58	1136018.5
59	898311.5
60	725475.5
61	646215.0
62	613360.0
63	567885.5
64	460709.0
65	364017.5
66	316910.0
67	284762.5
68	231010.5
69	170339.0
70	120364.0
71	86584.5
72	65036.0
73	50002.0
74	41665.0
75	29468.5
76	17046.5
77	13954.5
78	10513.5
79	7386.0
80	6030.0
81	3722.0
82	1488.5
83	280.5
84	209.0
85	161.5
86	139.0
87	131.5
88	112.5
89	81.0
90	75.0
91	67.0
92	51.5
93	58.0
94	62.0
95	83.0
96	150.5
97	365.0
98	1065.0
99	1241.5
100	477.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.07561379439482376
2	0.0499354915146403
3	0.04122199939649
4	0.03945771442735523
5	0.03947708696819279
6	0.039454946921521294
7	0.039454946921521294
8	0.039454946921521294
9	0.03945633067443827
10-11	0.039454946921521294
12-13	0.03947777884465127
14-15	0.03956149589612787
16-17	0.03946324943902311
18-19	0.039660434229691115
20-21	0.03948954074444551
22-23	0.04007279259894771
24-25	0.042527570273649735
26-27	0.04454854140888215
28-29	0.04655636689140336
30-31	0.048006539948386294
32-33	0.049385449730145345
34-35	0.050715236283352026
36-37	1.246024481016786E-4
38-39	2.1459582354577358E-5
40-41	2.561343856543035E-5
42-43	3.4890153440041404E-4
44-45	7.615025935947608E-5
46-47	1.7237875278323323E-4
48-49	1.4815101476002945E-4
50-51	5.6076684103976975E-5
52-53	9.692411729164052E-5
54-55	3.3923990156891944E-5
56-57	6.923378910213635E-7
58-59	0.0
60-61	0.0
62-63	3.461875901234481E-6
64-65	5.539109746388329E-6
66-67	3.4620153594804074E-6
68-69	0.0
70-71	2.7697281399656633E-6
72-73	6.2320357242446055E-6
74-75	0.0
76-77	2.7699327895198154E-6
78-79	0.0
80-81	1.385046960986745E-6
82-83	5.678871873542105E-5
84-85	6.233125544013346E-6
86-87	5.540758613381108E-6
88-89	2.6735191502098865E-4
90-91	4.571567040434063E-5
92-93	8.313203949121031E-6
94-95	5.972231603391E-4
96-97	2.150278616455802E-5
98-99	1.552810780555418E-5
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
34-35	37316.0
36-37	871.0
38-39	1087.0
40-41	988.0
42-43	1102.0
44-45	952.0
46-47	1035.0
48-49	1077.0
50-51	1095.0
52-53	1137.0
54-55	1198.0
56-57	1261.0
58-59	1198.0
60-61	1361.0
62-63	1418.0
64-65	1461.0
66-67	1511.0
68-69	1500.0
70-71	1689.0
72-73	1738.0
74-75	1842.0
76-77	1951.0
78-79	2164.0
80-81	2261.0
82-83	2460.0
84-85	2605.0
86-87	2727.0
88-89	3307.0
90-91	10427.0
92-93	6894.0
94-95	24397.0
96-97	526435.0
98-99	6543845.0
100-101	6.5074927E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.422569978655606
#Duplication Level	Percentage of deduplicated	Percentage of total
1	58.52379841814707	11.952063686113426
2	16.551635008100448	6.760538484281946
3	7.7958354930616025	4.776329876974131
4	4.441500374703013	3.628274088503895
5	2.6542647086761324	2.710345337740712
6	1.8243520029394475	2.235477386743883
7	1.276139333591532	1.8243431389951597
8	0.9321025188993791	1.5228743135602982
9	0.7359920428422141	1.3527764098810933
>10	4.456943239757866	17.06202130230776
>50	0.4192112543760627	5.961649647148409
>100	0.30887620637603286	12.78369754822955
>500	0.03981379273184174	5.674662112965825
>1k	0.035500656225137664	14.520183711047041
>5k	0.003053060703070788	4.136319353053521
>10k+	9.818888693292986E-4	3.0984436024531345
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.3837529169684459E-6	0.0	1.3837529169684459E-6	0.0
3	2.7675058339368918E-6	1.3837529169684459E-6	0.0	1.3837529169684459E-6	8.302517501810675E-6
4	1.2453776252716012E-5	1.3837529169684459E-6	2.7675058339368918E-6	1.3837529169684459E-6	8.302517501810675E-6
5	3.0442564173305808E-5	1.3837529169684459E-6	6.9187645848422294E-6	1.3837529169684459E-6	1.2453776252716012E-5
6	4.289634042602182E-5	1.3837529169684459E-6	1.2453776252716012E-5	1.3837529169684459E-6	1.2453776252716012E-5
7	4.428009334299027E-5	1.3837529169684459E-6	1.5221282086652904E-5	1.3837529169684459E-6	1.2453776252716012E-5
8	5.396636376176939E-5	1.3837529169684459E-6	2.2140046671495134E-5	1.3837529169684459E-6	1.2453776252716012E-5
9	5.673386959570628E-5	1.3837529169684459E-6	2.352379958846358E-5	1.3837529169684459E-6	2.0756293754526687E-5
10-11	6.849576938993807E-5	1.3837529169684459E-6	2.6983181880884694E-5	1.3837529169684459E-6	2.4907552505432028E-5
12-13	8.44089279350752E-5	1.3837529169684459E-6	3.1826317090274255E-5	1.3837529169684459E-6	3.5285699382695365E-5
14-15	9.755458064627544E-5	1.3837529169684459E-6	3.805320521663226E-5	1.3837529169684459E-6	4.012883459208493E-5
16-17	1.1277586273292833E-4	1.3837529169684459E-6	4.3588216884506045E-5	1.3837529169684459E-6	4.012883459208493E-5
18-19	1.238458860686759E-4	1.3837529169684459E-6	4.843135209389561E-5	1.3837529169684459E-6	4.428009334299027E-5
20-21	1.3076465065351813E-4	2.075629375452669E-6	5.189073438631672E-5	1.3837529169684459E-6	4.704759917692716E-5
22-23	1.3629966232139192E-4	2.7675058339368918E-6	5.811762251267473E-5	1.3837529169684459E-6	5.1198857927832495E-5
24-25	1.4875343857410794E-4	5.5350116678737835E-6	6.296075772206429E-5	1.3837529169684459E-6	5.189073438631672E-5
26-27	1.598234619098555E-4	9.68627041877912E-6	6.91876458484223E-5	1.3837529169684459E-6	5.7425746054190505E-5
28-29	1.92341655458614E-4	9.68627041877912E-6	8.025766918416986E-5	1.3837529169684459E-6	6.365263418054851E-5
30-31	2.0617918462829845E-4	1.176189979423179E-5	8.302517501810675E-5	1.3837529169684459E-6	7.403078105781185E-5
32-33	2.2831923129979358E-4	1.3837529169684459E-5	8.302517501810675E-5	1.3837529169684459E-6	8.44089279350752E-5
34-35	2.469998956788676E-4	1.3837529169684459E-5	1.0793272752353878E-4	1.3837529169684459E-6	8.717643376901209E-5
36-37	2.739830775597523E-4	1.3837529169684459E-5	1.1692712148383368E-4	2.7675058339368918E-6	9.478707481233855E-5
38-39	3.00966259440637E-4	1.452940562816868E-5	1.2246213315170745E-4	2.7675058339368918E-6	1.0931648044050722E-4
40-41	3.168794179857741E-4	1.5221282086652904E-5	1.3145652711200236E-4	2.7675058339368918E-6	1.2038650377625478E-4
42-43	3.38327588198785E-4	1.660503500362135E-5	1.3975904461381304E-4	2.7675058339368918E-6	1.2453776252716012E-4
44-45	3.563163761193748E-4	2.144817021301091E-5	1.4321842690623416E-4	2.7675058339368918E-6	1.2592151544412857E-4
46-47	3.8814269320964904E-4	2.2140046671495134E-5	1.5221282086652906E-4	2.7675058339368918E-6	1.3076465065351813E-4
48-49	4.1512587509053375E-4	2.2140046671495134E-5	1.5774783253440282E-4	4.151258750905338E-6	1.3491590940442348E-4
50-51	4.3934155113748157E-4	2.2140046671495134E-5	1.5843970899288704E-4	4.151258750905338E-6	1.3699153877987615E-4
52-53	4.697841153107874E-4	2.2140046671495134E-5	1.6259096774379238E-4	4.84313520938956E-6	1.3768341523836037E-4
54-55	5.106048263613565E-4	2.2140046671495134E-5	1.7642849691347685E-4	5.5350116678737835E-6	1.4114279753078149E-4
56-57	5.43123019910115E-4	2.2140046671495134E-5	1.9372540837558243E-4	5.5350116678737835E-6	1.5082906794956059E-4
58-59	5.839437309606842E-4	2.2140046671495134E-5	2.0479543171132998E-4	5.5350116678737835E-6	1.6674222649469772E-4
60-61	6.282238243036744E-4	2.2140046671495134E-5	2.2831923129979355E-4	5.5350116678737835E-6	1.6950973232863462E-4
62-63	6.607420178524328E-4	2.352379958846358E-5	2.6083742484855205E-4	6.9187645848422294E-6	1.7366099107953996E-4
64-65	6.960277172351283E-4	2.352379958846358E-5	2.719074481842996E-4	6.9187645848422294E-6	1.812716321228664E-4
66-67	7.271621578669183E-4	2.352379958846358E-5	2.7605870693520496E-4	6.9187645848422294E-6	1.840391379568033E-4
68-69	7.548372162062872E-4	2.352379958846358E-5	2.8782060672943676E-4	6.9187645848422294E-6	1.9026602608316132E-4
70-71	7.832041510041404E-4	2.352379958846358E-5	2.8782060672943676E-4	6.9187645848422294E-6	2.0410355525284578E-4
72-73	8.323273795565202E-4	2.352379958846358E-5	2.919718654803421E-4	6.9187645848422294E-6	2.1517357858859333E-4
74-75	8.814506081089E-4	2.352379958846358E-5	2.94739371314279E-4	8.302517501810675E-6	2.17249207964046E-4
76-77	9.312657131197641E-4	2.352379958846358E-5	2.94739371314279E-4	8.302517501810675E-6	2.200167137979829E-4
78-79	9.74162053545786E-4	2.4907552505432024E-5	2.94739371314279E-4	8.302517501810675E-6	2.3247049005069892E-4
80-81	0.0010101396293869655	2.5599428963916248E-5	2.9612312423124744E-4	8.302517501810675E-6	2.3662174880160426E-4
82-83	0.0010295121702245237	2.629130542240047E-5	3.037337652745739E-4	8.302517501810675E-6	2.442323898449307E-4
84-85	0.0010571872285638927	2.629130542240047E-5	3.0580939465002657E-4	8.302517501810675E-6	2.532267838052256E-4
86-87	0.001064797869607219	2.629130542240047E-5	3.141119121518372E-4	8.302517501810675E-6	2.5461053672219404E-4
88-89	0.0010682572518996402	2.629130542240047E-5	3.1549566506880564E-4	8.302517501810675E-6	2.5737804255613093E-4
90	0.0010682572518996402	2.629130542240047E-5	3.168794179857741E-4	8.302517501810675E-6	2.587617954730994E-4
>>END_MODULE
