##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	SRR21290308_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	74571019
Sequences flagged as poor quality	0
Sequence length	35-101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.92364197142056	37.0	37.0	37.0	37.0	37.0
2	36.090542345948094	37.0	37.0	37.0	37.0	37.0
3	36.233567627659745	37.0	37.0	37.0	37.0	37.0
4	36.27693708999739	37.0	37.0	37.0	37.0	37.0
5	36.29958311284442	37.0	37.0	37.0	37.0	37.0
6	36.266839722278704	37.0	37.0	37.0	37.0	37.0
7	36.235845027141174	37.0	37.0	37.0	37.0	37.0
8	36.29287428672525	37.0	37.0	37.0	37.0	37.0
9	36.276531637578934	37.0	37.0	37.0	37.0	37.0
10-11	36.2967628751325	37.0	37.0	37.0	37.0	37.0
12-13	36.280007237127876	37.0	37.0	37.0	37.0	37.0
14-15	36.277125286433325	37.0	37.0	37.0	37.0	37.0
16-17	36.278387774210245	37.0	37.0	37.0	37.0	37.0
18-19	36.27664671579719	37.0	37.0	37.0	37.0	37.0
20-21	36.269044828259624	37.0	37.0	37.0	37.0	37.0
22-23	36.256104948760324	37.0	37.0	37.0	37.0	37.0
24-25	36.22350003021951	37.0	37.0	37.0	37.0	37.0
26-27	36.17660887804148	37.0	37.0	37.0	37.0	37.0
28-29	36.160938252701094	37.0	37.0	37.0	37.0	37.0
30-31	36.15214595632655	37.0	37.0	37.0	37.0	37.0
32-33	36.14954241137566	37.0	37.0	37.0	37.0	37.0
34-35	36.13548438971982	37.0	37.0	37.0	37.0	37.0
36-37	36.14930819151485	37.0	37.0	37.0	37.0	37.0
38-39	36.14546242678419	37.0	37.0	37.0	37.0	37.0
40-41	36.148069640578214	37.0	37.0	37.0	37.0	37.0
42-43	36.128762793710585	37.0	37.0	37.0	37.0	37.0
44-45	36.12497854165281	37.0	37.0	37.0	37.0	37.0
46-47	36.12391218843202	37.0	37.0	37.0	37.0	37.0
48-49	36.09793090698378	37.0	37.0	37.0	37.0	37.0
50-51	36.091602495432525	37.0	37.0	37.0	37.0	37.0
52-53	36.09341910734918	37.0	37.0	37.0	37.0	37.0
54-55	36.084235540081195	37.0	37.0	37.0	37.0	37.0
56-57	36.06729760868734	37.0	37.0	37.0	37.0	37.0
58-59	36.050500338417535	37.0	37.0	37.0	37.0	37.0
60-61	36.05070947895564	37.0	37.0	37.0	37.0	37.0
62-63	36.03877203420245	37.0	37.0	37.0	37.0	37.0
64-65	36.023039392908196	37.0	37.0	37.0	37.0	37.0
66-67	36.02391680623842	37.0	37.0	37.0	37.0	37.0
68-69	36.00742489652513	37.0	37.0	37.0	37.0	37.0
70-71	35.98987117542427	37.0	37.0	37.0	37.0	37.0
72-73	35.96852169997108	37.0	37.0	37.0	37.0	37.0
74-75	35.965948394679984	37.0	37.0	37.0	37.0	37.0
76-77	35.95272077750329	37.0	37.0	37.0	37.0	37.0
78-79	35.937612928829594	37.0	37.0	37.0	37.0	37.0
80-81	35.907947715320205	37.0	37.0	37.0	37.0	37.0
82-83	35.92251363994224	37.0	37.0	37.0	37.0	37.0
84-85	35.90636874940517	37.0	37.0	37.0	37.0	37.0
86-87	35.91960213148816	37.0	37.0	37.0	37.0	37.0
88-89	35.89451579558095	37.0	37.0	37.0	37.0	37.0
90-91	35.87223549620248	37.0	37.0	37.0	37.0	37.0
92-93	35.86327041515121	37.0	37.0	37.0	37.0	37.0
94-95	35.8359445070241	37.0	37.0	37.0	37.0	37.0
96-97	35.840144238954096	37.0	37.0	37.0	37.0	37.0
98-99	35.82066435792464	37.0	37.0	37.0	37.0	37.0
100-101	35.79524081824629	37.0	37.0	37.0	37.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	63117.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	4.0
15	70.0
16	394.0
17	1014.0
18	1801.0
19	2688.0
20	3829.0
21	6438.0
22	11767.0
23	23860.0
24	46345.0
25	83353.0
26	135921.0
27	222720.0
28	340965.0
29	504583.0
30	694006.0
31	960987.0
32	1289802.0
33	1878725.0
34	3003814.0
35	6987590.0
36	4.4242696E7
37	1.406453E7
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.692849941967655	16.99387056097808	11.39031282633376	44.922966670720506
2	21.94976828692998	19.253601433385487	30.98082168392191	27.81580859576262
3	23.24567366712607	19.012865351023642	23.036117830796112	34.70534315105417
4	24.41523617743573	23.8063343107733	21.358104021746634	30.420325490044338
5	25.55340854935566	27.522692766311817	23.76984024938891	23.154058434943607
6	22.017718335432395	32.419871384917	23.435151884963826	22.12725839468678
7	19.088751150180016	22.918958313978564	36.969407620684315	21.02288291515711
8	19.75657696011894	24.54696550172625	29.486571773286546	26.209885764868268
9	20.37851422466611	22.647268276249903	32.05196630155997	24.92225119752402
10-11	22.20496290447153	30.210909709952645	24.876594297340436	22.707533088235394
12-13	21.77886973679188	25.959937340322263	27.77339106825259	24.48780185463326
14-15	21.78702212995452	26.335529008459314	27.33301135377239	24.544437507813775
16-17	22.655948071006435	26.32032753039124	26.802777074832136	24.220947323770186
18-19	22.435998694498355	26.77694399807966	26.10835187195105	24.67870543547093
20-21	22.473754016639074	26.527507286243036	26.477952794172353	24.520785902945537
22-23	22.37108064052677	26.544092810431252	26.646936837367768	24.43788971167421
24-25	22.53462743599213	26.226073336027365	26.773692909016013	24.46560631896449
26-27	22.731186995536458	26.535765697385294	26.518440371713332	24.214606935364912
28-29	22.4461293589327	26.364783587762297	26.67268552337225	24.51640152993275
30-31	22.477716932146365	25.95857171240371	26.82108800049739	24.742623354952535
32-33	22.80509256825113	26.137936725798948	26.585099009371383	24.471871696578535
34-35	22.706974703246562	26.324970927186992	26.42401547579042	24.544038893776023
36-37	22.292805195309878	26.294128466653287	26.697839653487616	24.71522668454922
38-39	22.666529666391984	26.386795213892857	26.820848891341452	24.12582622837371
40-41	22.99775474773666	26.284256507519903	26.1272754388652	24.590713305878236
42-43	22.669243325648626	26.474042083963845	26.38612180831663	24.470592782070902
44-45	22.88702254292241	26.2603775464288	26.634299452186905	24.21830045846189
46-47	22.74481042278909	25.924238304986012	26.69529952196253	24.635651750262365
48-49	22.516092092427883	25.725676497363075	26.86246782274561	24.895763587463428
50-51	22.72984703408682	25.681652374222917	26.68939825177153	24.89910233991873
52-53	22.971159979285904	26.142551416365173	26.353154688958224	24.533133915390696
54-55	22.59165421416876	26.079908281361025	26.626293071658598	24.702144432811618
56-57	22.700724507282683	26.26697413327091	26.477176587078134	24.555124772368277
58-59	22.6105233680343	26.189485025817977	26.472250574857043	24.72774103129068
60-61	22.737956028212935	26.051928600436703	26.406782799547635	24.803332571802724
62-63	22.81417846889168	25.837115294398842	26.511389700335414	24.83731653637406
64-65	22.84454410887112	26.093363692392103	26.241663116866647	24.820429081870127
66-67	22.911014596206048	25.978308388880826	26.326072077464296	24.784604937448826
68-69	22.780915063567985	26.123373512495984	26.51977001631145	24.575941407624576
70-71	23.33917717310837	26.009024458404394	26.31381725744314	24.33798111104409
72-73	23.062690298338808	25.824570072422976	26.34973661943163	24.763003009806585
74-75	23.284494467482194	25.971883627616055	26.30247237232513	24.441149532576624
76-77	23.191404432187518	26.010490786967218	26.329976837551733	24.46812794329353
78-79	22.854425191628376	26.169226896507126	26.05298552972755	24.92336238213695
80-81	23.038186689872013	26.074026379206284	26.084065191874885	24.803721739046818
82-83	23.115901652259428	26.209359693837193	25.761621002677987	24.913117651225395
84-85	23.041094111461977	26.252491102723685	25.966296296219195	24.740118489595147
86-87	23.062681101971688	26.1653697604711	25.970950348871565	24.800998788685654
88-89	23.154738555305222	25.90709751308004	25.94384570736335	24.994318224251387
90-91	23.065877761471263	25.87128274736849	26.400529205487544	24.6623102856727
92-93	23.482328593776273	25.726144203494254	26.258986996729394	24.53254020600008
94-95	23.22001665935979	25.8391179676269	26.2915370764006	24.649328296612712
96-97	23.222755936894586	25.666787941981273	26.380191230155525	24.730264890968616
98-99	23.468595166426308	24.780216616752966	26.78750452632358	24.96368369049714
100-101	24.358945443629114	11.412133517508646	32.47410457011509	31.754816468747155
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	63273.0
1	31669.5
2	73.5
3	84.5
4	90.5
5	108.0
6	129.0
7	133.0
8	143.0
9	143.0
10	164.5
11	208.5
12	247.5
13	306.5
14	421.0
15	638.5
16	958.0
17	1412.0
18	2080.5
19	3051.0
20	4100.0
21	6168.5
22	10544.5
23	15474.5
24	20990.0
25	29457.0
26	35414.5
27	43972.0
28	60620.5
29	79198.0
30	106886.0
31	143946.5
32	207791.0
33	306905.5
34	415702.5
35	539570.5
36	736690.5
37	1025380.0
38	1365717.5
39	1841908.5
40	2332647.5
41	2751291.5
42	3248398.0
43	3766336.0
44	4163047.0
45	4503640.5
46	4636572.5
47	4559599.0
48	4424916.5
49	4272785.5
50	4059230.0
51	3798623.5
52	3377933.0
53	2749156.0
54	2256245.5
55	1958758.0
56	1716414.5
57	1444526.5
58	1222498.0
59	1001946.0
60	815526.0
61	718437.0
62	662728.5
63	606356.5
64	509851.5
65	407451.0
66	349013.5
67	313916.0
68	258816.0
69	193902.5
70	141923.0
71	101342.5
72	76079.5
73	61052.0
74	52790.0
75	39066.0
76	21875.5
77	17575.0
78	13175.5
79	8719.0
80	7223.5
81	4934.5
82	2033.0
83	308.5
84	215.0
85	172.5
86	146.5
87	127.5
88	102.5
89	78.5
90	69.5
91	66.5
92	56.0
93	61.5
94	82.5
95	112.5
96	224.5
97	603.5
98	1837.5
99	2098.0
100	759.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12087939954260248
2	0.09450588304284806
3	0.08634051252538201
4	0.08464146104802457
5	0.08466425810809962
6	0.08464012004449074
7	0.08464012004449074
8	0.08464012004449074
9	0.08464280205155839
10-11	0.08464012004449074
12-13	0.08466425810809962
14-15	0.08472996728125708
16-17	0.08464950706922753
18-19	0.08483389505512859
20-21	0.0846736451328364
22-23	0.0851966365110285
24-25	0.08803285898507032
26-27	0.09034206707031857
28-29	0.09259830551598068
30-31	0.09424706936081965
32-33	0.0959568488664477
34-35	0.09752314099395637
36-37	1.1611162335204216E-4
38-39	2.0806425786737774E-5
40-41	2.3491513439075347E-5
42-43	3.2016060168607444E-4
44-45	7.047678721161779E-5
46-47	1.597500896446354E-4
48-49	1.3558862320432418E-4
50-51	6.309676238228485E-5
52-53	9.531798398302105E-5
54-55	3.35633161117497E-5
56-57	1.3425590140106974E-6
58-59	6.712933637388054E-7
60-61	0.0
62-63	8.05585451540279E-6
64-65	1.342671614411566E-6
66-67	8.056223254219946E-6
68-69	0.0
70-71	6.713867437466988E-7
72-73	6.042655467504441E-6
74-75	0.0
76-77	5.371578290360785E-6
78-79	0.0
80-81	2.0144774180002435E-6
82-83	4.969231125238472E-5
84-85	6.715444566896099E-6
86-87	2.0147158471967776E-6
88-89	2.4782151955948805E-4
90-91	3.761231616904912E-5
92-93	7.389351167071101E-6
94-95	5.603073063871204E-4
96-97	2.152457560011105E-5
98-99	1.6422653632864105E-5
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
34-35	73502.0
36-37	1009.0
38-39	1233.0
40-41	1104.0
42-43	1234.0
44-45	1257.0
46-47	1307.0
48-49	1272.0
50-51	1243.0
52-53	1429.0
54-55	1437.0
56-57	1528.0
58-59	1482.0
60-61	1608.0
62-63	1570.0
64-65	1754.0
66-67	1935.0
68-69	1874.0
70-71	2170.0
72-73	2153.0
74-75	2283.0
76-77	2422.0
78-79	2505.0
80-81	2833.0
82-83	2885.0
84-85	2989.0
86-87	3365.0
88-89	4034.0
90-91	11901.0
92-93	7551.0
94-95	29201.0
96-97	539705.0
98-99	6680308.0
100-101	6.7176936E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.299736827586916
#Duplication Level	Percentage of deduplicated	Percentage of total
1	56.133399311925594	9.710930353341611
2	16.869456582899197	5.836743186171195
3	8.224517991648044	4.268459903677945
4	4.654397171346422	3.2207938462143226
5	2.8654992490208127	2.4786191443854
6	1.945677193664255	2.0195822041097684
7	1.4341226554658906	1.7366961162628005
8	1.0220983240721129	1.4145625614692157
9	0.8020526210644237	1.248776933966174
>10	5.040856586742932	16.643815933468574
>50	0.5024233035070345	6.061292346763909
>100	0.40056562554274283	14.102724069590899
>500	0.05330300506050926	6.399180168403147
>1k	0.04643647452970915	15.977638020289328
>5k	0.0037001196909418606	4.2549750385543526
>10k+	0.0014937838195449738	4.625210173331347
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTGAACTTGTTTCAGGAAACATGAGGTCTTGGAACTTGATCTTTCTCT	90254	0.12103093294192481	No Hit
CTTTTATCGTTGAACTTGTTTCAGGAAACATGAGGTCTTGGAACTTGATC	87272	0.1170320604040559	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.6820070676518446E-6
2	0.0	2.6820070676518446E-6	0.0	0.0	2.6820070676518446E-6
3	1.3410035338259223E-6	2.6820070676518446E-6	0.0	0.0	5.364014135303689E-6
4	1.0728028270607378E-5	2.6820070676518446E-6	1.3410035338259223E-6	0.0	6.705017669129612E-6
5	3.218408481182214E-5	2.6820070676518446E-6	1.3410035338259223E-6	1.3410035338259223E-6	8.046021202955535E-6
6	3.754809894712583E-5	2.6820070676518446E-6	5.364014135303689E-6	1.3410035338259223E-6	8.046021202955535E-6
7	3.754809894712583E-5	2.6820070676518446E-6	6.705017669129612E-6	1.3410035338259223E-6	9.387024736781457E-6
8	4.9617130751559126E-5	2.6820070676518446E-6	6.705017669129612E-6	1.3410035338259223E-6	9.387024736781457E-6
9	5.095813428538505E-5	2.6820070676518446E-6	6.705017669129612E-6	1.3410035338259223E-6	1.609204240591107E-5
10-11	6.235666432290539E-5	2.6820070676518446E-6	1.0728028270607378E-5	2.0115053007388832E-6	2.1456056541214757E-5
12-13	7.710770319499054E-5	2.6820070676518446E-6	2.0115053007388836E-5	2.6820070676518446E-6	2.8831575977257333E-5
14-15	1.0325727210459603E-4	2.6820070676518446E-6	2.6149568909605487E-5	2.6820070676518446E-6	3.2854586578735096E-5
16-17	1.2672483394654966E-4	3.352508834564806E-6	3.218408481182214E-5	4.693512368390729E-6	3.5536593646386945E-5
18-19	1.3544135691641817E-4	4.023010601477767E-6	4.0900607781690635E-5	6.705017669129612E-6	3.888910248095175E-5
20-21	1.4348737811937368E-4	4.023010601477767E-6	4.760562545082025E-5	6.705017669129612E-6	4.157110954860359E-5
22-23	1.5220390108924218E-4	4.023010601477767E-6	5.2299137819210974E-5	6.705017669129612E-6	4.425311661625544E-5
24-25	1.7097795056280512E-4	4.023010601477767E-6	5.7663151954514665E-5	9.387024736781457E-6	4.6935123683907283E-5
26-27	1.8036497529958656E-4	6.705017669129612E-6	6.302716608981835E-5	9.387024736781457E-6	4.9617130751559126E-5
28-29	2.112080565775828E-4	9.387024736781457E-6	8.046021202955534E-5	9.387024736781457E-6	5.095813428538505E-5
30-31	2.3936913078792716E-4	1.2739533571346263E-5	8.31422190972072E-5	9.387024736781457E-6	5.69926501876017E-5
32-33	2.6887120853209747E-4	1.4751038872085147E-5	8.515372439794606E-5	1.1398530037520339E-5	6.503867139055723E-5
34-35	2.836222474041826E-4	1.4751038872085147E-5	9.454074913472753E-5	1.2069031804433301E-5	6.705017669129612E-5
36-37	3.151358304490918E-4	1.4751038872085147E-5	9.789325796929232E-5	1.3410035338259224E-5	6.839118022512205E-5
38-39	3.3458038168956766E-4	1.609204240591107E-5	1.0392777387150898E-4	1.3410035338259224E-5	7.777820496190349E-5
40-41	3.5804794353152126E-4	1.609204240591107E-5	1.106327915406386E-4	1.3410035338259224E-5	8.314221909720719E-5
42-43	3.815155053734749E-4	1.609204240591107E-5	1.159968056759423E-4	1.3410035338259224E-5	8.314221909720719E-5
44-45	4.1168808488455816E-4	1.609204240591107E-5	1.1666730744285524E-4	1.3410035338259224E-5	8.381272086412015E-5
46-47	4.425311661625544E-4	1.676254417282403E-5	1.247133286458108E-4	1.3410035338259224E-5	8.448322263103311E-5
48-49	4.955008057486783E-4	1.743304593973699E-5	1.260543321796367E-4	1.3410035338259224E-5	8.515372439794606E-5
50-51	5.330489046958041E-4	1.9444551240475872E-5	1.2873633924728855E-4	1.4751038872085147E-5	8.649472793177199E-5
52-53	5.618804806730615E-4	2.1456056541214757E-5	1.3879386575098296E-4	1.4751038872085147E-5	8.716522969868496E-5
54-55	5.954055690187096E-4	2.1456056541214757E-5	1.4281687635246074E-4	1.609204240591107E-5	8.850623323251088E-5
56-57	6.289306573643576E-4	2.1456056541214757E-5	1.4952189402159033E-4	1.676254417282403E-5	9.31997456009016E-5
58-59	6.604442404092668E-4	2.1456056541214757E-5	1.6427293289367548E-4	1.743304593973699E-5	1.0996228977372563E-4
60-61	7.033563534916962E-4	2.1456056541214757E-5	1.7567146293119584E-4	1.8774049473562915E-5	1.1264429684137747E-4
62-63	7.556554913109073E-4	2.1456056541214757E-5	1.863994912018032E-4	1.8774049473562915E-5	1.1264429684137747E-4
64-65	8.046021202955534E-4	2.1456056541214757E-5	1.9444551240475875E-4	1.8774049473562915E-5	1.1800831097668116E-4
66-67	8.609242687162422E-4	2.279706007504068E-5	1.9444551240475875E-4	2.0115053007388836E-5	1.2337232511198487E-4
68-69	9.051773853324976E-4	2.279706007504068E-5	1.9578651593858467E-4	2.0115053007388836E-5	1.327593498487663E-4
70-71	9.568060213847956E-4	2.279706007504068E-5	1.9578651593858467E-4	2.1456056541214757E-5	1.3946436751789592E-4
72-73	0.001019162685707701	2.279706007504068E-5	2.0316203537462725E-4	2.1456056541214757E-5	1.468398869539385E-4
74-75	0.0010734733288276508	2.279706007504068E-5	2.038325371415402E-4	2.1456056541214757E-5	1.4751038872085146E-4
76-77	0.0011170559436769934	2.279706007504068E-5	2.091965512768439E-4	2.1456056541214757E-5	1.4751038872085146E-4
78-79	0.0011626500638270746	2.279706007504068E-5	2.091965512768439E-4	2.1456056541214757E-5	1.4751038872085146E-4
80-81	0.0012109261910448078	2.279706007504068E-5	2.0986705304375686E-4	2.1456056541214757E-5	1.4818089048776443E-4
82-83	0.001240428268788978	2.279706007504068E-5	2.1053755481066983E-4	2.1456056541214757E-5	1.4952189402159035E-4
84-85	0.0012665778376985837	2.279706007504068E-5	2.1053755481066983E-4	2.1456056541214757E-5	1.5488590815689404E-4
86-87	0.0012712713500669746	2.279706007504068E-5	2.1053755481066983E-4	2.1456056541214757E-5	1.575679152245459E-4
88-89	0.0012726123536008003	2.279706007504068E-5	2.1187855834449573E-4	2.1456056541214757E-5	1.6024992229219772E-4
90	0.0012726123536008003	2.279706007504068E-5	2.1187855834449573E-4	2.1456056541214757E-5	1.6092042405911069E-4
>>END_MODULE
