##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	SRR21290290_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	86029065
Sequences flagged as poor quality	0
Sequence length	35-101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.00379539170861	37.0	37.0	37.0	37.0	37.0
2	36.13456133691561	37.0	37.0	37.0	37.0	37.0
3	36.25787981073606	37.0	37.0	37.0	37.0	37.0
4	36.29175378112037	37.0	37.0	37.0	37.0	37.0
5	36.31182520698092	37.0	37.0	37.0	37.0	37.0
6	36.278671167703614	37.0	37.0	37.0	37.0	37.0
7	36.24374067066752	37.0	37.0	37.0	37.0	37.0
8	36.30725541420217	37.0	37.0	37.0	37.0	37.0
9	36.2888808334718	37.0	37.0	37.0	37.0	37.0
10-11	36.30889671996319	37.0	37.0	37.0	37.0	37.0
12-13	36.287972361433894	37.0	37.0	37.0	37.0	37.0
14-15	36.284121593091825	37.0	37.0	37.0	37.0	37.0
16-17	36.283285271088324	37.0	37.0	37.0	37.0	37.0
18-19	36.28064063581303	37.0	37.0	37.0	37.0	37.0
20-21	36.274680591960404	37.0	37.0	37.0	37.0	37.0
22-23	36.26134226264112	37.0	37.0	37.0	37.0	37.0
24-25	36.22993220953872	37.0	37.0	37.0	37.0	37.0
26-27	36.185742545266535	37.0	37.0	37.0	37.0	37.0
28-29	36.171913294652214	37.0	37.0	37.0	37.0	37.0
30-31	36.16287722643504	37.0	37.0	37.0	37.0	37.0
32-33	36.15768901475333	37.0	37.0	37.0	37.0	37.0
34-35	36.144631049982934	37.0	37.0	37.0	37.0	37.0
36-37	36.17692911299537	37.0	37.0	37.0	37.0	37.0
38-39	36.17164575334033	37.0	37.0	37.0	37.0	37.0
40-41	36.17160419464853	37.0	37.0	37.0	37.0	37.0
42-43	36.15435801796376	37.0	37.0	37.0	37.0	37.0
44-45	36.153139158836424	37.0	37.0	37.0	37.0	37.0
46-47	36.15021639360249	37.0	37.0	37.0	37.0	37.0
48-49	36.12463377276639	37.0	37.0	37.0	37.0	37.0
50-51	36.119240989790924	37.0	37.0	37.0	37.0	37.0
52-53	36.12084659857709	37.0	37.0	37.0	37.0	37.0
54-55	36.1112343448754	37.0	37.0	37.0	37.0	37.0
56-57	36.09593984221236	37.0	37.0	37.0	37.0	37.0
58-59	36.08338941562415	37.0	37.0	37.0	37.0	37.0
60-61	36.08027509632571	37.0	37.0	37.0	37.0	37.0
62-63	36.06955162831849	37.0	37.0	37.0	37.0	37.0
64-65	36.054509111123224	37.0	37.0	37.0	37.0	37.0
66-67	36.054895093999384	37.0	37.0	37.0	37.0	37.0
68-69	36.03812906098548	37.0	37.0	37.0	37.0	37.0
70-71	36.01885775612889	37.0	37.0	37.0	37.0	37.0
72-73	35.99556920568696	37.0	37.0	37.0	37.0	37.0
74-75	35.99172016838187	37.0	37.0	37.0	37.0	37.0
76-77	35.98146221890345	37.0	37.0	37.0	37.0	37.0
78-79	35.96855291435069	37.0	37.0	37.0	37.0	37.0
80-81	35.93897223499151	37.0	37.0	37.0	37.0	37.0
82-83	35.95184276032272	37.0	37.0	37.0	37.0	37.0
84-85	35.93478635289622	37.0	37.0	37.0	37.0	37.0
86-87	35.94949454410285	37.0	37.0	37.0	37.0	37.0
88-89	35.92547005619477	37.0	37.0	37.0	37.0	37.0
90-91	35.90611186643041	37.0	37.0	37.0	37.0	37.0
92-93	35.89484099245402	37.0	37.0	37.0	37.0	37.0
94-95	35.86811371797333	37.0	37.0	37.0	37.0	37.0
96-97	35.87116092338362	37.0	37.0	37.0	37.0	37.0
98-99	35.85030407522028	37.0	37.0	37.0	37.0	37.0
100-101	35.82225906759601	37.0	37.0	37.0	37.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	114255.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	9.0
15	69.0
16	354.0
17	1024.0
18	1805.0
19	2750.0
20	4167.0
21	7120.0
22	13076.0
23	26671.0
24	50970.0
25	93073.0
26	153751.0
27	254332.0
28	389540.0
29	577409.0
30	789942.0
31	1081727.0
32	1432725.0
33	2052028.0
34	3256648.0
35	7594978.0
36	5.1195708E7
37	1.6934934E7
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.265174132933222	16.10369639873916	11.73291167500666	46.89821779332096
2	21.74393350727682	19.59198758290189	32.867486115240624	25.796592794580665
3	23.20779700710506	20.161720529492573	23.68213349292373	32.948348970478634
4	24.69049368870723	25.489310591130117	20.961948968819772	28.858246751342875
5	25.870650101650128	29.368153645713758	23.20013683324275	21.561059419393366
6	21.269953340989755	33.77913074590981	23.202277930894567	21.748637982205864
7	18.760724722547835	21.737991389377452	38.411067893882326	21.090215994192388
8	19.859591146159783	24.015869906480617	29.379410837316637	26.74512811004296
9	20.812692052170846	22.079699510699005	31.630585303306162	25.47702313382399
10-11	22.797890142572623	30.40840223006953	23.948971079607812	22.84473654775003
12-13	22.09106462238848	25.623537278930236	27.535646917886353	24.749751180794934
14-15	22.12022905359163	26.189683211638847	27.010032539119216	24.6800551956503
16-17	22.95353693696913	26.217378366860057	26.411041729071027	24.418042967099794
18-19	22.723190477561992	26.6047881143046	25.93947190825861	24.732549499874796
20-21	22.7613885887449	26.41759233924616	26.246281889848234	24.574737182160707
22-23	22.650428260194005	26.535193637524873	26.386795176200035	24.42758292608109
24-25	22.64682103568008	26.345025549057922	26.467006799308322	24.541146615953675
26-27	22.7635501558518	26.572572670994845	26.353170921741743	24.310706251411617
28-29	22.626209726107046	26.372410646512822	26.372422868893235	24.628956758486893
30-31	22.688417864868516	26.10018298912653	26.504037900148624	24.70736124585633
32-33	22.86934221321977	26.308010991560128	26.35782823008474	24.464818565135367
34-35	22.846088914653183	26.37995991457675	26.186951001999383	24.587000168770683
36-37	22.624630297533987	26.282904488600558	26.392710192497315	24.69975502136814
38-39	22.986535172843382	26.40305283353378	26.411351930038187	24.199060063584653
40-41	23.088049423412535	26.31240659855829	26.050122952007683	24.549421026021495
42-43	22.85068658595259	26.341084446918707	26.25983426847234	24.548394698656363
44-45	22.985347405916883	26.288146543834472	26.39980951012416	24.32669654012448
46-47	22.947168373604736	26.089300159786582	26.372783278231392	24.590748188377294
48-49	22.754280058173716	25.930707540324722	26.501591137422214	24.813421264079345
50-51	23.005698481681684	25.94689258727489	26.388614157578953	24.658794773464475
52-53	23.232043994664597	26.20875099974776	26.06111927623123	24.498085729356415
54-55	22.7538906809653	26.171980227709323	26.38863515343109	24.685493937894293
56-57	22.91270438744551	26.299669936563074	26.31115766825884	24.476468007732578
58-59	22.921543166297155	26.22877020540802	26.234711833816597	24.614974794478222
60-61	22.96667584534132	26.09347293069353	26.226421078726798	24.71343014523835
62-63	23.092188870402357	25.962337987179122	26.32696422425437	24.618508918164142
64-65	23.096134036708747	26.190866587282112	26.06177581113528	24.65122356487386
66-67	23.165732661211933	26.078622739949488	26.114149744519384	24.641494854319195
68-69	23.125435895616945	26.22131033490233	26.281142872500485	24.37211089698024
70-71	23.449895284265885	26.128498237285918	26.114286782956203	24.30731969549199
72-73	23.214091842311678	26.002653571020947	26.12606428061177	24.6571903060556
74-75	23.27745450930048	26.176389249374527	26.161014243788312	24.38514199753668
76-77	23.304145132510033	26.129208899389827	26.123317843569616	24.443328124530527
78-79	23.103327125227576	26.17560372535259	26.00722757557753	24.713841573842302
80-81	23.312480449384022	26.23056250877825	26.009796794485418	24.447160247352308
82-83	23.360277833665506	26.263401434080258	25.801004544169594	24.575316188084642
84-85	23.21796560022977	26.2875594105228	25.9175477492817	24.57692723996573
86-87	23.23774424181082	26.249092751901877	25.95358833686852	24.55957466941878
88-89	23.371022940446316	26.011018802508623	25.921506371464815	24.696451885580245
90-91	23.282612903212655	26.001988946633524	26.174836493477997	24.540561656675823
92-93	23.543974405732367	25.986054942591558	26.11578893829686	24.35418171337922
94-95	23.3975536831389	26.069114797379417	26.095237174163227	24.438094345318458
96-97	23.38929256399912	25.87204949920401	26.15414232666009	24.58451561013678
98-99	23.575093494657505	25.06670331182976	26.58483327958092	24.773369913931813
100-101	24.422528911807834	11.662830602102124	32.518371956298814	31.396268529791232
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	114338.0
1	57194.0
2	58.0
3	78.5
4	99.0
5	124.5
6	140.0
7	142.0
8	144.5
9	149.5
10	177.5
11	224.5
12	280.5
13	371.0
14	519.5
15	714.0
16	1036.0
17	1540.0
18	2207.5
19	3181.0
20	4328.5
21	5980.0
22	9068.0
23	13752.5
24	19062.5
25	25814.5
26	34437.5
27	48752.5
28	67535.5
29	89826.0
30	126156.5
31	173335.5
32	251511.0
33	361899.0
34	489640.5
35	649983.5
36	889459.5
37	1214383.0
38	1621485.0
39	2171243.0
40	2714853.5
41	3205112.5
42	3738981.0
43	4287141.0
44	4722974.5
45	5035171.0
46	5215993.0
47	5243807.5
48	5102799.0
49	4871208.5
50	4567825.0
51	4208188.5
52	3760289.0
53	3180366.0
54	2691145.5
55	2341659.5
56	2044578.0
57	1732083.5
58	1433856.5
59	1144845.0
60	940435.0
61	837072.5
62	782261.5
63	717990.0
64	610223.5
65	516084.5
66	455994.0
67	401654.5
68	326666.5
69	246545.0
70	180722.5
71	134095.5
72	101224.0
73	78232.0
74	65201.0
75	47348.5
76	29216.5
77	24216.0
78	17482.5
79	11363.0
80	9502.0
81	6118.0
82	2508.5
83	447.0
84	324.0
85	254.0
86	211.5
87	178.0
88	134.0
89	91.5
90	87.5
91	78.5
92	72.5
93	77.5
94	80.5
95	95.0
96	188.0
97	510.5
98	1503.5
99	1759.0
100	681.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.16890919365449342
2	0.1429784224668721
3	0.13459637158674223
4	0.13281325328829274
5	0.13282720206246576
6	0.13280976609474948
7	0.13280976609474948
8	0.13280976609474948
9	0.1328144156861405
10-11	0.13280976609474948
12-13	0.13283185165385675
14-15	0.1328899715462443
16-17	0.13281557808398825
18-19	0.13301144212133423
20-21	0.13284173203556263
22-23	0.13340200779817846
24-25	0.1361063263909703
26-27	0.1384235665004612
28-29	0.14075010579273411
30-31	0.14250358294606594
32-33	0.1442099829865639
34-35	0.1459030154518127
36-37	1.466782407439744E-4
38-39	3.201361773152564E-5
40-41	3.4342498517699185E-5
42-43	3.486695265639438E-4
44-45	1.0244885774849787E-4
46-47	2.0199066397986874E-4
48-49	1.4669319399637613E-4
50-51	6.112323230084106E-5
52-53	1.0129175866267495E-4
54-55	3.4346690922493846E-5
56-57	1.1643167852850292E-6
58-59	5.82170132666036E-7
60-61	0.0
62-63	2.9109689688844894E-6
64-65	4.0754414040720036E-6
66-67	1.0479941252709764E-5
68-69	0.0
70-71	1.746737227320272E-6
72-73	4.075823807982677E-6
74-75	0.0
76-77	2.9114636990699477E-6
78-79	0.0
80-81	1.7469880121449672E-6
82-83	5.881723182300621E-5
84-85	1.0482641549263606E-5
86-87	2.3295624007883053E-6
88-89	2.6266819588091075E-4
90-91	4.484854811300112E-5
92-93	5.825426157157264E-6
94-95	5.808563992943684E-4
96-97	2.4497851354702305E-5
98-99	1.6615889300198086E-5
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
34-35	126429.0
36-37	1339.0
38-39	1556.0
40-41	1430.0
42-43	1434.0
44-45	1470.0
46-47	1483.0
48-49	1501.0
50-51	1531.0
52-53	1575.0
54-55	1643.0
56-57	1696.0
58-59	1693.0
60-61	1783.0
62-63	1759.0
64-65	1919.0
66-67	1974.0
68-69	1943.0
70-71	2136.0
72-73	2280.0
74-75	2427.0
76-77	2523.0
78-79	2747.0
80-81	2904.0
82-83	2867.0
84-85	3202.0
86-87	3218.0
88-89	4069.0
90-91	13159.0
92-93	8751.0
94-95	30651.0
96-97	618301.0
98-99	7721235.0
100-101	7.7454437E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.852187441310072
#Duplication Level	Percentage of deduplicated	Percentage of total
1	58.28928823045773	12.154591640020536
2	16.186761376786247	6.7505876459301035
3	7.680954803432395	4.804941278682097
4	4.323570197861589	3.606235847258877
5	2.751745719947194	2.8689958771580804
6	1.8833835700853447	2.356360033638201
7	1.3939860437066476	2.0347360791758886
8	1.0066269258977427	1.6792298673831578
9	0.8052624058049357	1.5112334362756332
>10	4.833781886105433	18.951944251464038
>50	0.44612072770488015	6.453707285334946
>100	0.3263800392589632	13.504161352771698
>500	0.0385333749394989	5.578584663494693
>1k	0.030672999665734307	12.452014914862776
>5k	0.0023314583827605735	3.2501526753305727
>10k+	6.002399630413401E-4	2.0425231512187523
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	114255	0.13280976609474948	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.324795695501282E-6	1.162397847750641E-6	2.324795695501282E-6	0.0	1.162397847750641E-6
2	3.487193543251923E-6	4.649591391002564E-6	4.649591391002564E-6	0.0	3.487193543251923E-6
3	1.2786376325257051E-5	5.811989238753205E-6	4.649591391002564E-6	0.0	1.046158062975577E-5
4	1.2786376325257051E-5	5.811989238753205E-6	6.974387086503846E-6	0.0	1.6273569868508974E-5
5	2.441035480276346E-5	6.974387086503846E-6	6.974387086503846E-6	2.324795695501282E-6	1.8598365564010256E-5
6	3.8359128975771154E-5	6.974387086503846E-6	1.162397847750641E-5	4.649591391002564E-6	1.9760763411760897E-5
7	4.0683924671272436E-5	1.162397847750641E-5	2.092316125951154E-5	5.811989238753205E-6	2.324795695501282E-5
8	4.417111821452436E-5	1.162397847750641E-5	2.6735150498264744E-5	5.811989238753205E-6	2.441035480276346E-5
9	4.5333516062275E-5	1.162397847750641E-5	2.6735150498264744E-5	5.811989238753205E-6	3.254713973701795E-5
10-11	6.27694837785346E-5	1.162397847750641E-5	3.603433328026987E-5	5.811989238753205E-6	4.0683924671272436E-5
12-13	7.497466117991635E-5	1.3367575249132372E-5	4.765831175777628E-5	5.811989238753205E-6	5.2307903148778846E-5
14-15	9.64790213633032E-5	1.3948774173007692E-5	5.695749453978141E-5	5.811989238753205E-6	6.044468808303333E-5
16-17	1.1391498907956281E-4	1.3948774173007692E-5	7.67182579515423E-5	5.811989238753205E-6	6.27694837785346E-5
18-19	1.2030817724219134E-4	1.3948774173007692E-5	8.543624180967211E-5	6.974387086503846E-6	6.625667732178653E-5
20-21	1.255389675570692E-4	1.6273569868508974E-5	9.415422566780192E-5	6.974387086503846E-6	7.09062687127891E-5
22-23	1.3425695141519904E-4	1.8017166640134936E-5	1.0694060199305897E-4	6.974387086503846E-6	7.148746763666442E-5
24-25	1.4646212881658076E-4	1.9760763411760897E-5	1.0926539768856026E-4	6.974387086503846E-6	7.323106440829038E-5
26-27	1.6622289222834166E-4	2.324795695501282E-5	1.1449618800343813E-4	6.974387086503846E-6	7.671825795154231E-5
28-29	2.0341962335636217E-4	2.6735150498264744E-5	1.226329729376926E-4	6.974387086503846E-6	8.601744073354742E-5
30-31	2.295735749307516E-4	2.6735150498264744E-5	1.325133546435731E-4	6.974387086503846E-6	9.64790213633032E-5
32-33	2.545651286573904E-4	2.7897548346015385E-5	1.3716294603457563E-4	6.974387086503846E-6	1.0461580629755769E-4
34-35	2.7665068776465255E-4	2.9059946193766026E-5	1.5576131159858588E-4	6.974387086503846E-6	1.0926539768856026E-4
36-37	3.0454823611066794E-4	2.9059946193766026E-5	1.6854768792384296E-4	6.974387086503846E-6	1.1449618800343813E-4
38-39	3.30120988761182E-4	2.9641145117641346E-5	1.761032739342221E-4	6.974387086503846E-6	1.3076975787194712E-4
40-41	3.4930055324906763E-4	3.370953758476859E-5	1.8191526317297532E-4	8.717983858129808E-6	1.4123133850170287E-4
42-43	3.725485102040805E-4	3.429073650864391E-5	1.9412044057435704E-4	1.162397847750641E-5	1.452997309688301E-4
44-45	4.0393325209334775E-4	3.5453134356394555E-5	1.9702643519373364E-4	1.162397847750641E-5	1.476245266643314E-4
46-47	4.35317993982615E-4	3.719673112802051E-5	1.9993242981311026E-4	1.220517740138173E-5	1.4820572558820673E-4
48-49	4.7425832188226154E-4	3.719673112802051E-5	2.010948276608609E-4	1.2786376325257051E-5	1.5401771482695994E-4
50-51	5.073866605431548E-4	3.8359128975771154E-5	2.0283842443248685E-4	1.2786376325257051E-5	1.638980965328404E-4
52-53	5.445833916711753E-4	3.8359128975771154E-5	2.2143678999649713E-4	1.2786376325257051E-5	1.638980965328404E-4
54-55	5.922417034289516E-4	3.8359128975771154E-5	2.353855641695048E-4	1.3948774173007692E-5	1.7145368254321955E-4
56-57	6.317632302524734E-4	3.8359128975771154E-5	2.4817194049476187E-4	1.3948774173007692E-5	1.9818883304148428E-4
58-59	6.701223592282446E-4	3.8359128975771154E-5	2.545651286573904E-4	1.3948774173007692E-5	2.2085559107262178E-4
60-61	7.212678645292728E-4	3.8359128975771154E-5	2.778130856124032E-4	1.4529973096883013E-5	2.2201798892037243E-4
62-63	7.753193644496775E-4	4.126512359514775E-5	3.144286178165484E-4	1.5111172020758333E-5	2.2201798892037243E-4
64-65	8.264648697507058E-4	4.300872036677372E-5	3.3128338660893267E-4	1.5692370944633654E-5	2.382915587888814E-4
66-67	8.75866778280108E-4	4.5333516062275E-5	3.3418938122830926E-4	1.6273569868508974E-5	2.4875313941863717E-4
68-69	9.386362620586427E-4	4.765831175777628E-5	3.4232616616256374E-4	1.6273569868508974E-5	2.5688992435289165E-4
70-71	9.909441652074214E-4	5.1145505301028205E-5	3.4406976293418974E-4	1.8017166640134936E-5	2.772318866885279E-4
72-73	0.0010333716866503199	5.1145505301028205E-5	3.4697575755356634E-4	1.8598365564010256E-5	2.923430587092862E-4
74-75	0.0010978847672004804	5.1145505301028205E-5	3.475569564774417E-4	1.8598365564010256E-5	2.998986447196654E-4
76-77	0.001146124277882132	5.2307903148778846E-5	3.48138155401317E-4	1.8598365564010256E-5	3.0919782750167047E-4
78-79	0.0011902953960966564	5.347030099652949E-5	3.4930055324906763E-4	1.8598365564010256E-5	3.150098167404237E-4
80-81	0.0012472528906364378	5.695749453978141E-5	3.4988175217294293E-4	1.8598365564010256E-5	3.225654027508029E-4
82-83	0.0012867744174599595	5.928229023528269E-5	3.510441500206936E-4	1.8598365564010256E-5	3.353517790760599E-4
84-85	0.0013210651539686034	6.044468808303333E-5	3.539501446400702E-4	1.8598365564010256E-5	3.4232616616256374E-4
86-87	0.0013379199227609878	6.160708593078397E-5	3.580185371071974E-4	1.8598365564010256E-5	3.510441500206936E-4
88-89	0.0013402447184564892	6.160708593078397E-5	3.5918093495494806E-4	2.092316125951154E-5	3.626681284982E-4
90	0.0013402447184564892	6.393188162628525E-5	3.603433328026987E-4	2.092316125951154E-5	3.6383052634595065E-4
>>END_MODULE
