##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	SRR21290309_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	77072011
Sequences flagged as poor quality	0
Sequence length	35-101
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.99732503411647	37.0	37.0	37.0	37.0	37.0
2	36.11710488519626	37.0	37.0	37.0	37.0	37.0
3	36.245743659134575	37.0	37.0	37.0	37.0	37.0
4	36.282697035114346	37.0	37.0	37.0	37.0	37.0
5	36.300974344629466	37.0	37.0	37.0	37.0	37.0
6	36.26931654864955	37.0	37.0	37.0	37.0	37.0
7	36.23536674033327	37.0	37.0	37.0	37.0	37.0
8	36.294527879907015	37.0	37.0	37.0	37.0	37.0
9	36.27528404572186	37.0	37.0	37.0	37.0	37.0
10-11	36.29644131123035	37.0	37.0	37.0	37.0	37.0
12-13	36.277777019468196	37.0	37.0	37.0	37.0	37.0
14-15	36.27544838553648	37.0	37.0	37.0	37.0	37.0
16-17	36.276166343966295	37.0	37.0	37.0	37.0	37.0
18-19	36.27320812350413	37.0	37.0	37.0	37.0	37.0
20-21	36.266929534250764	37.0	37.0	37.0	37.0	37.0
22-23	36.25410879703139	37.0	37.0	37.0	37.0	37.0
24-25	36.22360465591069	37.0	37.0	37.0	37.0	37.0
26-27	36.178878905858575	37.0	37.0	37.0	37.0	37.0
28-29	36.16565526621591	37.0	37.0	37.0	37.0	37.0
30-31	36.157663201496064	37.0	37.0	37.0	37.0	37.0
32-33	36.15390921225605	37.0	37.0	37.0	37.0	37.0
34-35	36.13946752343078	37.0	37.0	37.0	37.0	37.0
36-37	36.173691680613764	37.0	37.0	37.0	37.0	37.0
38-39	36.169060327424205	37.0	37.0	37.0	37.0	37.0
40-41	36.172158068408436	37.0	37.0	37.0	37.0	37.0
42-43	36.15556314714223	37.0	37.0	37.0	37.0	37.0
44-45	36.152701306916654	37.0	37.0	37.0	37.0	37.0
46-47	36.148673312457504	37.0	37.0	37.0	37.0	37.0
48-49	36.12122822509019	37.0	37.0	37.0	37.0	37.0
50-51	36.11639641855126	37.0	37.0	37.0	37.0	37.0
52-53	36.12053124846868	37.0	37.0	37.0	37.0	37.0
54-55	36.11029094630375	37.0	37.0	37.0	37.0	37.0
56-57	36.094416254387255	37.0	37.0	37.0	37.0	37.0
58-59	36.07907428264895	37.0	37.0	37.0	37.0	37.0
60-61	36.076852060057504	37.0	37.0	37.0	37.0	37.0
62-63	36.06922517496679	37.0	37.0	37.0	37.0	37.0
64-65	36.05524502091285	37.0	37.0	37.0	37.0	37.0
66-67	36.053993505344195	37.0	37.0	37.0	37.0	37.0
68-69	36.03617111188143	37.0	37.0	37.0	37.0	37.0
70-71	36.01863995933304	37.0	37.0	37.0	37.0	37.0
72-73	35.998455186878765	37.0	37.0	37.0	37.0	37.0
74-75	35.99623146866497	37.0	37.0	37.0	37.0	37.0
76-77	35.983433504508184	37.0	37.0	37.0	37.0	37.0
78-79	35.96828622696018	37.0	37.0	37.0	37.0	37.0
80-81	35.939174757987765	37.0	37.0	37.0	37.0	37.0
82-83	35.95358669661174	37.0	37.0	37.0	37.0	37.0
84-85	35.93992651924622	37.0	37.0	37.0	37.0	37.0
86-87	35.953694576247926	37.0	37.0	37.0	37.0	37.0
88-89	35.92842488488923	37.0	37.0	37.0	37.0	37.0
90-91	35.90780712544385	37.0	37.0	37.0	37.0	37.0
92-93	35.89842386222507	37.0	37.0	37.0	37.0	37.0
94-95	35.87364911568787	37.0	37.0	37.0	37.0	37.0
96-97	35.87856741905818	37.0	37.0	37.0	37.0	37.0
98-99	35.857861209257734	37.0	37.0	37.0	37.0	37.0
100-101	35.83202587942395	37.0	37.0	37.0	37.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	111741.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	88.0
16	372.0
17	971.0
18	1545.0
19	2261.0
20	3269.0
21	5805.0
22	11282.0
23	23367.0
24	45065.0
25	82816.0
26	137646.0
27	227754.0
28	347259.0
29	514908.0
30	705565.0
31	966706.0
32	1281959.0
33	1848254.0
34	2932811.0
35	6841321.0
36	4.5856043E7
37	1.5123203E7
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.65233015885775	16.23886210480133	13.247297587812515	42.86151014852841
2	22.813902517321047	19.35244037161063	29.811507961647138	28.02214914942119
3	24.512154704445848	19.42526588793612	22.66583865788356	33.39674074973448
4	25.402220202738633	24.229524197738968	20.528943317492825	29.839312282029574
5	26.225177900924752	28.079067360642217	22.88983019442296	22.805924544010068
6	22.167817238686922	32.915807078119656	22.647151575741614	22.26922410745181
7	19.219212718458497	22.456666277288267	37.22760328153734	21.09651772271589
8	20.346350136245626	24.450307931611988	28.707338214899714	26.49600371724268
9	21.10364505092881	22.581641444106697	30.947789436650748	25.36692406831374
10-11	22.78440551209085	30.293418929013633	23.99680770350728	22.92536785538824
12-13	22.279449382004042	25.6854660137712	27.267495646535206	24.767588957689554
14-15	22.28381907581049	26.196225607031376	26.890953621606183	24.629001695551946
16-17	23.03251529362332	26.183241334230654	26.313611213496202	24.470632158649824
18-19	22.865090666285084	26.616534636692478	25.817663663108604	24.700711033913834
20-21	22.9036215113871	26.37610400507566	26.07940081375002	24.640873669787222
22-23	22.81419205953699	26.43360050538448	26.1649590766094	24.587248358469132
24-25	22.809302805451395	26.243359801706955	26.292489318395607	24.65484807444604
26-27	23.021425607558204	26.41867057194263	26.071743294124257	24.488160526374912
28-29	22.80889563485185	26.316808502699313	26.157235534837227	24.717060327611613
30-31	22.829473897046427	26.003908349674433	26.307782959182198	24.858834794096946
32-33	23.040281689498435	26.198308725458574	26.14770369727473	24.613705887768266
34-35	22.95615727460629	26.295345305721945	26.039796112924662	24.708701306747102
36-37	22.70793717666111	26.228018868157456	26.279921375313453	24.78412257986798
38-39	23.10408353457869	26.307719927276825	26.174179685969523	24.41401685217496
40-41	23.22148460464856	26.20311622706414	25.762263443288692	24.81313572499861
42-43	23.043449617091806	26.255451263127867	26.078653979099066	24.62244514068126
44-45	23.23922627319506	26.195930713110144	26.185762265289753	24.379080748405038
46-47	23.134363049378745	25.987713122410895	26.1585774042319	24.71934642397846
48-49	22.948537824545443	25.786430680382892	26.305029545666837	24.960001949404823
50-51	23.165182983670647	25.801550469865926	26.146559539173133	24.886707007290294
52-53	23.372255146724676	26.115964575127492	25.878494091690314	24.633286186457518
54-55	22.9955634209959	26.09919713625144	26.129673565914995	24.775565876837664
56-57	23.071120136378994	26.244903117338993	26.047472620541352	24.636504125740665
58-59	23.077872313062546	26.139071332576446	26.04154845423713	24.74150790012387
60-61	23.139224134653695	25.97087211757835	26.052178092526425	24.83772565524153
62-63	23.216648417426775	25.858399571880803	26.049007654282697	24.87594435640973
64-65	23.246494560557778	26.06522170530968	25.799526079918344	24.8887576542142
66-67	23.316182027988724	26.01816463647867	25.860461404266253	24.805191931266357
68-69	23.276395271088816	26.072723673360308	26.024983258098764	24.62589779745211
70-71	23.683148944942936	26.028614506184834	25.78415294970513	24.5040835991671
72-73	23.410191739417442	25.967593437677795	25.824346450284512	24.797868372620254
74-75	23.509014470047177	26.045798281845407	25.87382618036805	24.57136106773937
76-77	23.496855233084606	25.99746629891894	25.862352907492024	24.643325560504422
78-79	23.29722088753168	26.134992861636373	25.67893137298025	24.888854877851696
80-81	23.517271774852542	26.104914802831296	25.663482615907608	24.714330806408558
82-83	23.524564188244362	26.199108524440646	25.490069383393937	24.78625790392105
84-85	23.376308718725944	26.216679598238652	25.64916044979144	24.75785123324396
86-87	23.41402402644967	26.132016592315026	25.670723190706795	24.783236190528505
88-89	23.61373131681297	25.891928119464218	25.593112198460723	24.901228365262092
90-91	23.480144210645186	25.911257981319224	25.9016986645232	24.70689914351239
92-93	23.809554248087835	25.807432903742267	25.81406169840696	24.56895114976294
94-95	23.606408294378948	25.88558279440071	25.84715637259937	24.66085253862097
96-97	23.55242069757419	25.680323893695387	25.95630688856933	24.81094852016109
98-99	23.79720802521009	24.883635272112787	26.331101584810522	24.9880551178666
100-101	24.656357929408358	11.53676644514279	32.18598230306745	31.620893322381406
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	112005.0
1	56057.5
2	108.0
3	104.0
4	110.0
5	118.5
6	124.5
7	133.0
8	146.0
9	160.0
10	189.5
11	213.5
12	246.5
13	328.5
14	437.0
15	609.5
16	855.5
17	1228.0
18	1767.0
19	2516.0
20	3449.5
21	4825.5
22	7798.0
23	12400.5
24	17878.0
25	24997.5
26	32142.0
27	43350.0
28	59960.0
29	79743.5
30	111628.5
31	151832.0
32	217171.5
33	314388.0
34	424232.5
35	556983.5
36	760524.5
37	1040244.5
38	1372568.0
39	1819022.0
40	2270285.0
41	2685159.5
42	3166607.5
43	3645911.0
44	4044783.5
45	4376915.0
46	4546998.5
47	4557769.5
48	4461588.5
49	4300784.0
50	4101456.5
51	3873772.5
52	3493381.0
53	2939830.0
54	2488978.0
55	2204600.0
56	1970940.0
57	1696077.0
58	1427953.0
59	1157074.0
60	955456.5
61	856552.0
62	805284.5
63	745109.5
64	621778.0
65	506410.0
66	446608.0
67	396521.5
68	318412.5
69	237555.5
70	173705.0
71	126778.5
72	98085.0
73	77845.0
74	67645.5
75	52569.5
76	32276.0
77	26452.0
78	20887.0
79	15064.0
80	13502.5
81	9580.5
82	3902.5
83	434.5
84	296.0
85	221.5
86	163.0
87	132.0
88	100.0
89	85.0
90	80.0
91	72.0
92	64.5
93	55.0
94	58.0
95	79.0
96	136.0
97	327.0
98	1037.0
99	1210.5
100	440.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.18129019625555118
2	0.15508872604868193
3	0.14672641667543876
4	0.14498389045538204
5	0.1450059477493068
6	0.14498259296750413
7	0.14498259296750413
8	0.14498259296750413
9	0.14498389045538204
10-11	0.14498259296750413
12-13	0.14500529900536785
14-15	0.14508639199773832
16-17	0.1449936216144665
18-19	0.14518694730827772
20-21	0.14501632765233022
22-23	0.14530566744910808
24-25	0.14685421923141465
26-27	0.1482250151744451
28-29	0.14968793275680845
30-31	0.15084075073634706
32-33	0.15207531045219516
34-35	0.15328327166654573
36-37	1.1955345951196042E-4
38-39	2.144190954940977E-5
40-41	2.5340804252930283E-5
42-43	3.521768182338443E-4
44-45	9.096948046705915E-5
46-47	1.6764622747253817E-4
48-49	1.3840755081938636E-4
50-51	6.36813851351077E-5
52-53	1.0267156813801544E-4
54-55	3.704036007960766E-5
56-57	0.0
58-59	0.0
60-61	1.2997310232641974E-6
62-63	6.4987851950810536E-6
64-65	1.2997821688564303E-6
66-67	9.098662510258986E-6
68-69	0.0
70-71	2.599735201371329E-6
72-73	5.19959817765263E-6
74-75	0.0
76-77	1.2999671881781868E-6
78-79	0.0
80-81	1.3000467711326616E-6
82-83	6.955476241458254E-5
84-85	7.800806915467336E-6
86-87	6.500926203208486E-6
88-89	2.457452620996093E-4
90-91	5.591381247296592E-5
92-93	5.852378702249718E-6
94-95	5.879005718100453E-4
96-97	2.2787145508190065E-5
98-99	1.8539981858230465E-5
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
34-35	118770.0
36-37	896.0
38-39	1124.0
40-41	978.0
42-43	1096.0
44-45	1130.0
46-47	1095.0
48-49	1063.0
50-51	1172.0
52-53	1280.0
54-55	1314.0
56-57	1342.0
58-59	1384.0
60-61	1523.0
62-63	1492.0
64-65	1533.0
66-67	1681.0
68-69	1743.0
70-71	1904.0
72-73	1962.0
74-75	1960.0
76-77	2224.0
78-79	2389.0
80-81	2519.0
82-83	2612.0
84-85	2952.0
86-87	3091.0
88-89	3867.0
90-91	11814.0
92-93	7617.0
94-95	26965.0
96-97	538643.0
98-99	6892352.0
100-101	6.9428524E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.425819716589945
#Duplication Level	Percentage of deduplicated	Percentage of total
1	55.943788797538915	10.867539554439752
2	17.082567309543396	6.6368574570340595
3	8.237193371501194	4.800427002164157
4	4.645081037725856	3.609380272312521
5	2.940012755624566	2.8556078877618813
6	1.9945186500057415	2.324709582983273
7	1.3925815581381114	1.8936426802327206
8	1.0227982719933866	1.5894955870546603
9	0.817467485388815	1.429197839580352
>10	5.019508045318014	18.421802714301887
>50	0.4681770836173843	6.331017983149303
>100	0.35009241980610456	13.690859028789612
>500	0.04540055102258072	6.131863871432323
>1k	0.037806016301437755	14.267154749509892
>5k	0.0023887391791796724	3.0996221574280107
>10k+	6.179072955606724E-4	2.05082163182567
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	111741	0.14498259296750413	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.892463633782697E-6	0.0	3.892463633782697E-6	0.0	1.2974878779275657E-6
2	3.892463633782697E-6	0.0	7.784927267565393E-6	0.0	9.08241514549296E-6
3	7.784927267565393E-6	0.0	9.08241514549296E-6	0.0	1.2974878779275656E-5
4	2.8544733314406445E-5	0.0	9.08241514549296E-6	0.0	1.4272366657203222E-5
5	4.2817099971609665E-5	0.0	9.08241514549296E-6	0.0	1.5569854535130787E-5
6	4.670956360539237E-5	2.5949757558551314E-6	1.2974878779275656E-5	0.0	1.5569854535130787E-5
7	4.670956360539237E-5	2.5949757558551314E-6	1.9462318168913485E-5	0.0	1.5569854535130787E-5
8	4.800705148331993E-5	3.892463633782697E-6	1.9462318168913485E-5	1.2974878779275657E-6	1.5569854535130787E-5
9	5.060202723917506E-5	3.892463633782697E-6	3.3734684826116706E-5	1.2974878779275657E-6	1.6867342413058353E-5
10-11	6.357690601845072E-5	3.892463633782697E-6	4.022212421575454E-5	1.2974878779275657E-6	2.724724543647888E-5
12-13	7.784927267565394E-5	3.892463633782697E-6	5.125077117813884E-5	1.2974878779275657E-6	3.5680916643008056E-5
14-15	9.796033478353121E-5	5.189951511710263E-6	7.979550449254529E-5	1.2974878779275657E-6	4.54120757274648E-5
16-17	1.213151165862274E-4	5.189951511710263E-6	9.341912721078473E-5	1.9462318168913483E-6	4.54120757274648E-5
18-19	1.3169501960964792E-4	5.189951511710263E-6	1.057452620510966E-4	4.54120757274648E-6	4.54120757274648E-5
20-21	1.3883120293824955E-4	5.189951511710263E-6	1.096377256848793E-4	5.838695450674046E-6	4.670956360539237E-5
22-23	1.472648741447787E-4	5.189951511710263E-6	1.206663726472636E-4	7.784927267565393E-6	4.670956360539237E-5
24-25	1.6478096049680086E-4	5.189951511710263E-6	1.3169501960964792E-4	1.1677390901348092E-5	4.670956360539237E-5
26-27	1.8748699836053324E-4	9.08241514549296E-6	1.4142617869410465E-4	1.1677390901348092E-5	5.1899515117102625E-5
28-29	2.3679153772178076E-4	1.1677390901348092E-5	1.479136180837425E-4	1.1677390901348092E-5	5.579197875088532E-5
30-31	2.640387831582596E-4	1.2974878779275656E-5	1.505085938395976E-4	1.1677390901348092E-5	6.163067420155937E-5
32-33	2.7960863769339037E-4	1.88135742299497E-5	1.5894226504612678E-4	1.1677390901348092E-5	6.487439389637829E-5
34-35	2.9712472404541256E-4	2.011106210787727E-5	1.8813574229949702E-4	1.1677390901348092E-5	6.617188177430585E-5
36-37	3.16587042214326E-4	2.075980604684105E-5	1.9397443775017107E-4	1.1677390901348092E-5	6.941560146912476E-5
38-39	3.4188805583391355E-4	2.075980604684105E-5	2.1343675591908455E-4	1.2326134840311873E-5	7.849801661461773E-5
40-41	3.626478618807546E-4	2.2057293924768617E-5	2.3095284227110668E-4	1.2974878779275656E-5	8.239048024840043E-5
42-43	3.821101800496681E-4	2.3354781802696183E-5	2.4392772105038236E-4	1.2974878779275656E-5	8.69316878211469E-5
44-45	3.9573380276790754E-4	2.3354781802696183E-5	2.5365888013483906E-4	1.2974878779275656E-5	8.822917569907447E-5
46-47	4.268735118381691E-4	2.3354781802696183E-5	2.7117496648686125E-4	1.2974878779275656E-5	8.952666357700203E-5
48-49	4.729343315045977E-4	2.3354781802696183E-5	2.7960863769339037E-4	1.2974878779275656E-5	9.277038327182095E-5
50-51	5.034252966358955E-4	2.3354781802696183E-5	2.886910528388833E-4	1.2974878779275656E-5	9.536535902767608E-5
52-53	5.455936526685414E-4	2.3354781802696183E-5	2.977734679843763E-4	1.2974878779275656E-5	1.0444777417316904E-4
54-55	5.721921541660565E-4	2.3354781802696183E-5	3.0750462706883306E-4	1.2974878779275656E-5	1.1288144537969822E-4
56-57	6.098193026259559E-4	2.465226968062375E-5	3.16587042214326E-4	1.2974878779275656E-5	1.252075802200101E-4
58-59	6.513389147196379E-4	2.465226968062375E-5	3.211282497870725E-4	1.2974878779275656E-5	1.3947994687721333E-4
60-61	6.915610389353925E-4	2.465226968062375E-5	3.444830315897687E-4	1.2974878779275656E-5	1.4272366657203223E-4
62-63	7.278906995173643E-4	2.530101361958753E-5	3.743252527821027E-4	1.2974878779275656E-5	1.4337241051099603E-4
64-65	7.596791525265897E-4	2.724724543647888E-5	3.885976194393059E-4	1.2974878779275656E-5	1.472648741447787E-4
66-67	7.940625812916703E-4	2.724724543647888E-5	3.931388270120524E-4	1.2974878779275656E-5	1.4921110596167005E-4
68-69	8.518007918594469E-4	2.724724543647888E-5	3.9832877852376264E-4	1.2974878779275656E-5	1.5569854535130788E-4
70-71	9.049977948544771E-4	2.724724543647888E-5	3.9832877852376264E-4	1.2974878779275656E-5	1.7970207109296784E-4
72-73	9.841445554080587E-4	2.724724543647888E-5	4.1000616942511075E-4	1.4272366657203222E-5	1.9008197411638837E-4
74-75	0.001025015423562777	2.724724543647888E-5	4.1389863305889345E-4	1.4272366657203222E-5	1.9397443775017107E-4
76-77	0.0010613450841447489	2.724724543647888E-5	4.171423527537124E-4	1.4272366657203222E-5	1.9981313320084512E-4
78-79	0.0011041621841163586	2.789598937544266E-5	4.2168356032645885E-4	1.6867342413058353E-5	2.0630057259048292E-4
80-81	0.0011424380765152216	3.113970907026157E-5	4.229810482043864E-4	1.816483029098592E-5	2.114905241021932E-4
82-83	0.001174875273463411	3.243719694818914E-5	4.314147194109156E-4	1.816483029098592E-5	2.1668047561390348E-4
84-85	0.0012001762870829983	3.3085940887152924E-5	4.385509027395172E-4	1.816483029098592E-5	2.231679150035413E-4
86-87	0.001216394885557093	3.3734684826116706E-5	4.45038342129155E-4	1.816483029098592E-5	2.2446540288146885E-4
88-89	0.0012183411173739842	3.762714845989941E-5	4.45038342129155E-4	1.816483029098592E-5	2.2900661045421536E-4
90	0.0012183411173739842	3.762714845989941E-5	4.45038342129155E-4	1.816483029098592E-5	2.3095284227110668E-4
>>END_MODULE
