##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	SRR21290310_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	84987041
Sequences flagged as poor quality	0
Sequence length	35-101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.041288753658336	37.0	37.0	37.0	37.0	37.0
2	36.10188772191751	37.0	37.0	37.0	37.0	37.0
3	36.21670946279916	37.0	37.0	37.0	37.0	37.0
4	36.208740024258525	37.0	37.0	37.0	37.0	37.0
5	36.17499200848751	37.0	37.0	37.0	37.0	37.0
6	36.11111141050316	37.0	37.0	37.0	37.0	37.0
7	36.070419430181126	37.0	37.0	37.0	37.0	37.0
8	36.127146208090714	37.0	37.0	37.0	37.0	37.0
9	36.07992586775671	37.0	37.0	37.0	37.0	37.0
10-11	36.102672241524445	37.0	37.0	37.0	37.0	37.0
12-13	36.080677276433235	37.0	37.0	37.0	37.0	37.0
14-15	36.068991612497726	37.0	37.0	37.0	37.0	37.0
16-17	36.05010502130554	37.0	37.0	37.0	37.0	37.0
18-19	36.05413007613714	37.0	37.0	37.0	37.0	37.0
20-21	36.046737607913656	37.0	37.0	37.0	37.0	37.0
22-23	36.01982444005787	37.0	37.0	37.0	37.0	37.0
24-25	36.008257700135715	37.0	37.0	37.0	37.0	37.0
26-27	35.98139718148323	37.0	37.0	37.0	37.0	37.0
28-29	35.96580300989653	37.0	37.0	37.0	37.0	37.0
30-31	35.947229189918495	37.0	37.0	37.0	37.0	37.0
32-33	35.92804991881056	37.0	37.0	37.0	37.0	37.0
34-35	35.91143791557586	37.0	37.0	37.0	37.0	37.0
36-37	35.90070831245329	37.0	37.0	37.0	37.0	37.0
38-39	35.88996271908354	37.0	37.0	37.0	37.0	37.0
40-41	35.891838920281	37.0	37.0	37.0	37.0	37.0
42-43	35.8566712899895	37.0	37.0	37.0	37.0	37.0
44-45	35.845596066414245	37.0	37.0	37.0	37.0	37.0
46-47	35.84862203984143	37.0	37.0	37.0	37.0	37.0
48-49	35.82858765333806	37.0	37.0	37.0	37.0	37.0
50-51	35.81185071494427	37.0	37.0	37.0	37.0	37.0
52-53	35.79911007816866	37.0	37.0	37.0	37.0	37.0
54-55	35.79486157583966	37.0	37.0	37.0	37.0	37.0
56-57	35.762276210072564	37.0	37.0	37.0	37.0	37.0
58-59	35.781913079637455	37.0	37.0	37.0	37.0	37.0
60-61	35.75081529006691	37.0	37.0	37.0	37.0	37.0
62-63	35.73810490597858	37.0	37.0	37.0	37.0	37.0
64-65	35.72675254924385	37.0	37.0	37.0	37.0	37.0
66-67	35.70915583547503	37.0	37.0	37.0	37.0	37.0
68-69	35.67757594256945	37.0	37.0	37.0	37.0	37.0
70-71	35.68405492201771	37.0	37.0	37.0	37.0	37.0
72-73	35.6634279474279	37.0	37.0	37.0	37.0	37.0
74-75	35.67496999804496	37.0	37.0	37.0	37.0	37.0
76-77	35.65150589639012	37.0	37.0	37.0	37.0	37.0
78-79	35.60710658076224	37.0	37.0	37.0	37.0	37.0
80-81	35.603757795743064	37.0	37.0	37.0	37.0	37.0
82-83	35.573361822811805	37.0	37.0	37.0	37.0	37.0
84-85	35.53221096347373	37.0	37.0	37.0	37.0	37.0
86-87	35.49541552878161	37.0	37.0	37.0	37.0	37.0
88-89	35.51996101290507	37.0	37.0	37.0	37.0	37.0
90-91	35.46056321353429	37.0	37.0	37.0	37.0	37.0
92-93	35.50331334114564	37.0	37.0	37.0	37.0	37.0
94-95	35.45872666792714	37.0	37.0	37.0	37.0	37.0
96-97	35.43587839173142	37.0	37.0	37.0	37.0	37.0
98-99	35.430780867230055	37.0	37.0	37.0	37.0	37.0
100-101	35.422749711485565	37.0	37.0	37.0	37.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	16758.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	99.0
12	2986.0
13	22724.0
14	49873.0
15	59117.0
16	54235.0
17	50092.0
18	46682.0
19	49263.0
20	62071.0
21	91502.0
22	128823.0
23	163839.0
24	186365.0
25	209092.0
26	238574.0
27	296325.0
28	373446.0
29	497592.0
30	659993.0
31	946493.0
32	1380620.0
33	2295525.0
34	4268939.0
35	1.1210992E7
36	5.0353321E7
37	1.12717E7
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.013123308589115	16.798471982149735	12.269387794990388	45.91901691427076
2	22.63772850738087	19.913407704574862	31.48310587216539	25.96575791587888
3	24.107058184048455	20.108945728689985	23.27366292664089	32.510333160620675
4	25.132104318357605	25.009007614130486	20.88103181059158	28.977856256920326
5	26.34361662655576	29.095748798082198	23.172926474878512	21.387708100483525
6	21.86142416901709	33.62899247885841	22.72754694487694	21.782036407247553
7	19.37397845888946	22.056987437406196	37.823154641933534	20.745879461770812
8	20.17844400085122	24.188703512302226	29.117337045337248	26.515515441509308
9	21.135469208687937	22.333444505533777	31.393966288190427	25.137119997587863
10-11	23.00206429892664	30.477599383840833	23.86736889988275	22.65296741734978
12-13	22.34825023432021	25.833318298661734	27.358745494291338	24.459685972726717
14-15	22.37757741331356	26.3560961111715	26.856541160962326	24.409785314552614
16-17	23.142535795887824	26.36577612963483	26.283181856460224	24.208506218017117
18-19	22.91368898158436	26.802343324271842	25.812364234066028	24.471603460077766
20-21	22.970596401820508	26.656115875478154	26.10036487242411	24.272922850277226
22-23	22.774779937885466	26.771307223018486	26.285249892753132	24.168662946342916
24-25	22.776512439906178	26.56218959771773	26.353813756891746	24.307484205484347
26-27	22.942026203920555	26.78015035466644	26.183320577756287	24.094502863656718
28-29	22.760569101322893	26.630188854635172	26.172987649654395	24.43625439438754
30-31	22.758028787992117	26.428907667945012	26.271697790758093	24.541365753304785
32-33	22.99375886395101	26.621624028924924	26.121951558404916	24.26266554871915
34-35	22.947544701255275	26.686023028917273	25.99390127107651	24.372530998750946
36-37	22.680199963385718	26.568740875819085	26.234018056795122	24.517041104000075
38-39	23.010986112822106	26.69892562448655	26.23545156463369	24.054636698057656
40-41	23.13815293710499	26.61477744435972	25.830504231472872	24.416565387062413
42-43	22.885598823474883	26.655929231925974	26.11775109489964	24.340720849699505
44-45	23.052939316078163	26.588900036029155	26.235731322849748	24.12242932504293
46-47	23.01153151630488	26.38297999072367	26.221172467321562	24.384316025649888
48-49	22.85012795652722	26.26987165830036	26.30481440160159	24.57518598357083
50-51	23.0663436690092	26.293734904733633	26.169771658377034	24.470149767880137
52-53	23.285677244421155	26.510208042984996	25.874846564551323	24.32926814804253
54-55	22.849047779780555	26.459064617183586	26.1896931275462	24.502194475489656
56-57	23.012501585047136	26.590503486550375	26.113681399304273	24.283313529098216
58-59	23.03150404681889	26.46723401571085	26.072271292368566	24.4289906451017
60-61	23.12103109185805	26.349339659594474	26.03213988194142	24.497489366606057
62-63	23.189466796081206	26.264888205710722	26.108003686524828	24.43764131168324
64-65	23.216043790106585	26.470449217431536	25.860524369899355	24.45298262256252
66-67	23.270304745274515	26.37876755893897	25.931631587633586	24.41929610815293
68-69	23.240150140895512	26.517465374824656	26.0363776980924	24.206006786187434
70-71	23.578244611931048	26.405528202072126	25.855118537820122	24.161108648176704
72-73	23.299775186411374	26.336938225230362	25.912305120039075	24.45098146831919
74-75	23.37678658341983	26.46360542404284	25.936204435298464	24.223403557238864
76-77	23.429929805633417	26.41878487680985	25.835733648767306	24.315551668789432
78-79	23.20013642799448	26.497029118729365	25.77459714817467	24.528237305101488
80-81	23.394418578911633	26.504557679014777	25.764339709301133	24.33668403277246
82-83	23.45795351959045	26.519496167347768	25.5817915698593	24.44075874320248
84-85	23.32073006365601	26.534897148382296	25.70780455769905	24.436568230262644
86-87	23.374654833013324	26.491271943802218	25.723937306711008	24.41013591647345
88-89	23.52893910536139	26.2679217093963	25.660466869605308	24.542672315637
90-91	23.428330389695624	26.275904864442378	25.899769747103967	24.39599499875803
92-93	23.680558209838296	26.2517391213983	25.854009875141443	24.213692793621966
94-95	23.55528773073758	26.27955420978285	25.847803545664704	24.31735451381487
96-97	23.49831373852565	26.100174942892256	25.944755354865062	24.456755963717033
98-99	23.673897350235727	25.311393250393404	26.355488629360487	24.65922077001038
100-101	24.61320441872685	11.853112482795604	32.26619157080947	31.267491527668078
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	17043.0
1	8802.5
2	1093.0
3	2334.5
4	3788.5
5	5232.0
6	6556.5
7	7817.0
8	9106.0
9	10422.0
10	11639.5
11	12774.0
12	13690.0
13	14554.0
14	15504.5
15	16160.0
16	16883.0
17	17761.5
18	18921.5
19	20198.5
20	21299.0
21	23217.0
22	26847.5
23	31989.0
24	37755.5
25	45158.0
26	55245.0
27	71905.0
28	93227.5
29	117263.0
30	155626.5
31	206810.5
32	286529.0
33	400297.0
34	532443.0
35	692826.5
36	925595.5
37	1238803.5
38	1633860.0
39	2151417.0
40	2653921.0
41	3113461.0
42	3629692.0
43	4145275.5
44	4561686.5
45	4884077.0
46	5048420.5
47	5053404.0
48	4904755.5
49	4672906.5
50	4408397.5
51	4098902.5
52	3677824.5
53	3102754.5
54	2611439.0
55	2280659.5
56	2000183.5
57	1706958.0
58	1434077.0
59	1171694.0
60	981002.5
61	880567.5
62	819916.0
63	748948.0
64	644067.0
65	545964.0
66	479179.5
67	418678.5
68	341680.0
69	263779.0
70	198722.0
71	149482.5
72	113764.0
73	88570.0
74	74985.0
75	57101.0
76	36999.0
77	30581.5
78	23237.0
79	16960.0
80	14995.0
81	10904.5
82	5682.0
83	2544.5
84	2191.5
85	2004.5
86	1932.5
87	1847.5
88	1733.0
89	1649.5
90	1571.5
91	1531.5
92	1530.5
93	1526.5
94	1515.5
95	1578.5
96	1670.5
97	1900.0
98	2366.5
99	3533.5
100	19701.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.06600535721675496
2	0.03509829222081046
3	0.02046782638308351
4	0.020308978635931094
5	0.02025249943694357
6	0.021209115869794785
7	0.01973242014626677
8	0.020359574585024087
9	0.019718300346519887
10-11	0.019754776495866
12-13	0.019741833346098026
14-15	0.01973595009620349
16-17	0.020555486806512067
18-19	0.019781839445380856
20-21	0.019760659745760533
22-23	0.021603881937718013
24-25	0.022565204970484854
26-27	0.0246372855833397
28-29	0.026707601221226184
30-31	0.028458456389839484
32-33	0.030577014676861143
34-35	0.03201252765112742
36-37	2.112787966378964E-4
38-39	5.314420321717115E-4
40-41	6.644618265591842E-4
42-43	0.0010852870280202806
44-45	8.651866462149099E-4
46-47	5.950480069546809E-4
48-49	5.715163278153619E-4
50-51	0.0010059117829845801
52-53	3.820071096173109E-4
54-55	2.4722100923691857E-5
56-57	7.475660030880363E-5
58-59	1.7070786033349216E-5
60-61	4.1206303224599846E-5
62-63	4.120732105513323E-6
64-65	1.6483370701429058E-5
66-67	7.0644843422710175E-6
68-69	1.059703276371162E-5
70-71	1.7662240142304904E-5
72-73	0.0
74-75	8.831701333056999E-6
76-77	4.416004563817069E-5
78-79	5.417171074480396E-5
80-81	2.638034585563795E-4
82-83	0.0015634545059059186
84-85	1.5841366324323354E-4
86-87	0.001264428486510197
88-89	5.4537585734513E-4
90-91	0.00222584252291964
92-93	0.0010191556369775162
94-95	0.0011100107563223847
96-97	0.0018244698390302056
98-99	0.0016781417511452386
100-101	3.5286885432236865E-4
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
34-35	27924.0
36-37	1251.0
38-39	1528.0
40-41	1444.0
42-43	1717.0
44-45	1646.0
46-47	1673.0
48-49	1601.0
50-51	1766.0
52-53	1789.0
54-55	1929.0
56-57	1843.0
58-59	1969.0
60-61	2019.0
62-63	2310.0
64-65	2154.0
66-67	2423.0
68-69	2441.0
70-71	2812.0
72-73	2743.0
74-75	2929.0
76-77	3131.0
78-79	3404.0
80-81	3581.0
82-83	3710.0
84-85	4252.0
86-87	4304.0
88-89	5242.0
90-91	14499.0
92-93	9418.0
94-95	31451.0
96-97	612140.0
98-99	7638237.0
100-101	7.6585761E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.424616640916643
#Duplication Level	Percentage of deduplicated	Percentage of total
1	59.53524822496286	12.755198698416423
2	15.08141911521807	6.462272458890789
3	7.612900964586118	4.893104540745664
4	4.3626827648984765	3.7387522305553658
5	2.734059788602119	2.9288091422073
6	1.8444763593072095	2.3710319340834354
7	1.3105034815572987	1.9653924289266187
8	0.9960886739656017	1.7072654384057622
9	0.7595361113291242	1.4645493009143167
>10	4.914532759061578	19.966970744783385
>50	0.4550014226766947	6.794924663357249
>100	0.3258218576645272	13.863184220624818
>500	0.037592781416128054	5.566563162849007
>1k	0.028010689693648377	11.595500177561805
>5k	0.0017261771312971404	2.5107850763613837
>10k+	3.988279292527689E-4	1.415695781316562
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	3.5299499367203526E-6	0.0	3.5299499367203526E-6
2	1.1766499789067843E-6	0.0	4.706599915627137E-6	0.0	5.883249894533921E-6
3	5.883249894533921E-6	0.0	4.706599915627137E-6	0.0	7.059899873440705E-6
4	1.411979974688141E-5	0.0	9.413199831254274E-6	0.0	7.059899873440705E-6
5	2.823959949376282E-5	0.0	1.8826399662508548E-5	0.0	1.0589849810161058E-5
6	4.353604921955101E-5	0.0	2.1179699620322116E-5	0.0	1.0589849810161058E-5
7	4.353604921955101E-5	0.0	2.470964955704247E-5	0.0	1.0589849810161058E-5
8	5.1772599071898505E-5	0.0	2.7062949514856036E-5	0.0	1.0589849810161058E-5
9	5.412589902971207E-5	0.0	3.0592899451576385E-5	1.1766499789067843E-6	1.1766499789067842E-5
10-11	7.059899873440706E-5	0.0	4.2359399240644226E-5	1.1766499789067843E-6	1.4708124736334802E-5
12-13	8.11888485445681E-5	0.0	5.589087399807225E-5	1.7649749683601765E-6	1.6473099704694977E-5
14-15	9.589697328090291E-5	1.1766499789067843E-6	7.059899873440706E-5	3.5299499367203526E-6	2.470964955704247E-5
16-17	1.0825179805942415E-4	1.1766499789067843E-6	8.23654985234749E-5	4.118274926173745E-6	2.765127450430943E-5
18-19	1.1766499789067842E-4	1.1766499789067843E-6	8.88370734074622E-5	4.706599915627137E-6	2.823959949376282E-5
20-21	1.2354824778521234E-4	1.1766499789067843E-6	9.707362325980969E-5	4.706599915627137E-6	4.0006099282830664E-5
22-23	1.400213474899073E-4	1.1766499789067843E-6	1.1060509801723771E-4	6.471574883987312E-6	4.47126991984578E-5
24-25	1.712025719309371E-4	4.118274926173745E-6	1.1295839797505127E-4	1.0001524820707666E-5	4.706599915627137E-5
26-27	2.1826857108720848E-4	4.706599915627137E-6	1.2001829784849199E-4	1.1766499789067842E-5	4.706599915627137E-5
28-29	2.635695952751196E-4	4.706599915627137E-6	1.3296144761646661E-4	1.1766499789067842E-5	5.0007624103538324E-5
30-31	3.118122444102978E-4	5.883249894533921E-6	1.3296144761646661E-4	1.2354824778521234E-5	5.118427408244511E-5
32-33	3.565249436087556E-4	8.824874841800882E-6	1.400213474899073E-4	1.5296449725788193E-5	5.353757404025868E-5
34-35	4.077092176912007E-4	1.0589849810161058E-5	1.5472947222624212E-4	1.5296449725788193E-5	5.412589902971207E-5
36-37	4.7889654141506114E-4	1.2943149767974626E-5	1.6120104711022943E-4	1.5296449725788193E-5	5.647919898752564E-5
38-39	5.571437650123623E-4	1.2943149767974626E-5	1.7767414681492443E-4	1.5296449725788193E-5	6.76573737871401E-5
40-41	6.248011387995024E-4	1.2943149767974626E-5	1.9002897159344564E-4	1.5296449725788193E-5	7.942387357620794E-5
42-43	6.954001375339095E-4	1.3531474757428018E-5	1.9532389649852617E-4	1.5296449725788193E-5	8.001219856566133E-5
44-45	7.789422860362911E-4	1.411979974688141E-5	1.9767719645633975E-4	1.5884774715241583E-5	8.001219856566133E-5
46-47	8.607194595703126E-4	1.5884774715241586E-5	2.0120714639306007E-4	1.6473099704694977E-5	8.11888485445681E-5
48-49	9.713245575875503E-4	1.764974968360176E-5	2.0709039628759402E-4	1.706142469414837E-5	8.589544846019524E-5
50-51	0.0010748697557313473	1.8238074673055154E-5	2.153269461399415E-4	1.764974968360176E-5	8.707209843910203E-5
52-53	0.0011795916038540512	1.8826399662508548E-5	2.1709192110830165E-4	1.764974968360176E-5	8.824874841800881E-5
54-55	0.0012937266518080093	1.8826399662508548E-5	2.2650512093955595E-4	1.764974968360176E-5	8.883707340746221E-5
56-57	0.0014155099246248614	1.8826399662508548E-5	2.4003659569698396E-4	1.764974968360176E-5	9.589697328090291E-5
58-59	0.0015249383726631924	1.8826399662508548E-5	2.435665456337043E-4	1.764974968360176E-5	1.0531017311215718E-4
60-61	0.0016514282453956716	1.8826399662508548E-5	2.6709954521184E-4	1.8238074673055154E-5	1.1060509801723771E-4
62-63	0.0017867429929699517	1.8826399662508548E-5	3.10635594431391E-4	2.0591374630868726E-5	1.1648834791177163E-4
64-65	0.0019208810905653251	1.8826399662508548E-5	3.2240209422045886E-4	2.1179699620322116E-5	1.2295992279575893E-4
66-67	0.0020609024380552326	1.8826399662508548E-5	3.2357874419936564E-4	2.1179699620322116E-5	1.2766652271138608E-4
68-69	0.002177390785967004	1.941472465196194E-5	3.312269690622597E-4	2.1768024609775507E-5	1.3237312262701323E-4
70-71	0.00230329233371003	2.1179699620322116E-5	3.3299194403061994E-4	2.23563495992289E-5	1.5119952228952177E-4
72-73	0.0024221339815796153	2.1179699620322116E-5	3.394635189146073E-4	2.23563495992289E-5	1.5943607214186924E-4
74-75	0.002585688328647658	2.1179699620322116E-5	3.4005184390406063E-4	2.23563495992289E-5	1.6178937209968282E-4
76-77	0.0027062949514856036	2.1179699620322116E-5	3.4417011883023437E-4	2.23563495992289E-5	1.6826094698367013E-4
78-79	0.0027933670499247056	2.1179699620322116E-5	3.482883937564081E-4	2.23563495992289E-5	1.7296754689929728E-4
80-81	0.0028904406731845153	2.1179699620322116E-5	3.500533687247683E-4	2.23563495992289E-5	1.7590917184656424E-4
82-83	0.002953391447056028	2.1768024609775507E-5	3.565249436087556E-4	2.23563495992289E-5	1.8296907172000494E-4
84-85	0.003020460495853715	2.23563495992289E-5	3.577015935876624E-4	2.23563495992289E-5	1.8355739670945832E-4
86-87	0.0030545833452420117	2.3532999578135684E-5	3.6358484348219633E-4	2.23563495992289E-5	1.8944064660399225E-4
88-89	0.0030616432451154524	2.3532999578135684E-5	3.659381434400099E-4	2.23563495992289E-5	1.994421714246999E-4
90	0.0030616432451154524	2.470964955704247E-5	3.659381434400099E-4	2.23563495992289E-5	2.0356044635087367E-4
>>END_MODULE
