##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	SRR21290291_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	82754272
Sequences flagged as poor quality	0
Sequence length	35-101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.99723492220438	37.0	37.0	37.0	37.0	37.0
2	36.133677002197544	37.0	37.0	37.0	37.0	37.0
3	36.260983807579144	37.0	37.0	37.0	37.0	37.0
4	36.296182183803154	37.0	37.0	37.0	37.0	37.0
5	36.3132800322381	37.0	37.0	37.0	37.0	37.0
6	36.278623972427674	37.0	37.0	37.0	37.0	37.0
7	36.243786931023934	37.0	37.0	37.0	37.0	37.0
8	36.30579192334626	37.0	37.0	37.0	37.0	37.0
9	36.289500438116356	37.0	37.0	37.0	37.0	37.0
10-11	36.312206196436605	37.0	37.0	37.0	37.0	37.0
12-13	36.293108717094384	37.0	37.0	37.0	37.0	37.0
14-15	36.289737960597364	37.0	37.0	37.0	37.0	37.0
16-17	36.28962237744052	37.0	37.0	37.0	37.0	37.0
18-19	36.28733719632021	37.0	37.0	37.0	37.0	37.0
20-21	36.28006039374016	37.0	37.0	37.0	37.0	37.0
22-23	36.26820947684731	37.0	37.0	37.0	37.0	37.0
24-25	36.23701259193	37.0	37.0	37.0	37.0	37.0
26-27	36.19348245852492	37.0	37.0	37.0	37.0	37.0
28-29	36.179730389024506	37.0	37.0	37.0	37.0	37.0
30-31	36.17051464122602	37.0	37.0	37.0	37.0	37.0
32-33	36.16590125401623	37.0	37.0	37.0	37.0	37.0
34-35	36.15254542992052	37.0	37.0	37.0	37.0	37.0
36-37	36.189148935493634	37.0	37.0	37.0	37.0	37.0
38-39	36.18387142284716	37.0	37.0	37.0	37.0	37.0
40-41	36.184244735404846	37.0	37.0	37.0	37.0	37.0
42-43	36.1676596630106	37.0	37.0	37.0	37.0	37.0
44-45	36.1650395521964	37.0	37.0	37.0	37.0	37.0
46-47	36.1622788146712	37.0	37.0	37.0	37.0	37.0
48-49	36.138074436994	37.0	37.0	37.0	37.0	37.0
50-51	36.13135058371637	37.0	37.0	37.0	37.0	37.0
52-53	36.133031302469234	37.0	37.0	37.0	37.0	37.0
54-55	36.12411572023632	37.0	37.0	37.0	37.0	37.0
56-57	36.108557111899366	37.0	37.0	37.0	37.0	37.0
58-59	36.09755659882201	37.0	37.0	37.0	37.0	37.0
60-61	36.0957984018862	37.0	37.0	37.0	37.0	37.0
62-63	36.08442332854883	37.0	37.0	37.0	37.0	37.0
64-65	36.0680118286967	37.0	37.0	37.0	37.0	37.0
66-67	36.067992644359535	37.0	37.0	37.0	37.0	37.0
68-69	36.051563034584994	37.0	37.0	37.0	37.0	37.0
70-71	36.034166106467694	37.0	37.0	37.0	37.0	37.0
72-73	36.01034662666244	37.0	37.0	37.0	37.0	37.0
74-75	36.00620701610811	37.0	37.0	37.0	37.0	37.0
76-77	35.99444350981558	37.0	37.0	37.0	37.0	37.0
78-79	35.98180133729458	37.0	37.0	37.0	37.0	37.0
80-81	35.953678478838526	37.0	37.0	37.0	37.0	37.0
82-83	35.967157705507326	37.0	37.0	37.0	37.0	37.0
84-85	35.95080882370796	37.0	37.0	37.0	37.0	37.0
86-87	35.963697232860866	37.0	37.0	37.0	37.0	37.0
88-89	35.93939186314667	37.0	37.0	37.0	37.0	37.0
90-91	35.921183730898946	37.0	37.0	37.0	37.0	37.0
92-93	35.91131361082991	37.0	37.0	37.0	37.0	37.0
94-95	35.883417571443815	37.0	37.0	37.0	37.0	37.0
96-97	35.8855864652068	37.0	37.0	37.0	37.0	37.0
98-99	35.86417652871886	37.0	37.0	37.0	37.0	37.0
100-101	35.83879639672687	37.0	37.0	37.0	37.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	118236.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	10.0
15	64.0
16	274.0
17	749.0
18	1408.0
19	2103.0
20	3339.0
21	5972.0
22	11507.0
23	23897.0
24	46384.0
25	85467.0
26	143082.0
27	236679.0
28	364376.0
29	544444.0
30	746006.0
31	1027294.0
32	1362838.0
33	1964427.0
34	3117353.0
35	7246823.0
36	4.9128083E7
37	1.6573457E7
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.847425136785972	16.097077286995102	10.858427598615105	47.197069977603824
2	21.90310582609782	19.01433265024987	32.853679044364924	26.22888247928738
3	23.163882228203445	19.718336959641157	23.487046066866338	33.63073474528907
4	24.718214631624438	25.147330099933523	20.896189438328918	29.238265830113118
5	25.94638673931151	28.991249347696613	23.089272786896746	21.973091126095134
6	21.46425803870844	33.50240928788017	23.32082458553554	21.71250808787585
7	18.831908878107367	22.1070962309954	37.91712613126796	21.14386875962927
8	19.671898347108517	24.062579671658018	29.692064367656744	26.57345761357672
9	20.65715252846833	22.10540084473558	31.848180616988937	25.389266009807148
10-11	22.635174699299316	30.498955265103444	24.180422474405855	22.68544756119139
12-13	22.050751997447698	25.79118874174704	27.531392984525265	24.626666276279998
14-15	22.08180702543792	26.385175732032106	26.92522755136175	24.607789691168225
16-17	22.910793925826447	26.363545021135575	26.37114521665319	24.35451583638479
18-19	22.717535678556462	26.720298982008124	25.87190777690738	24.690257562528036
20-21	22.76916889268379	26.511457650658265	26.192142246176054	24.52723121048189
22-23	22.630456305639107	26.598177372939936	26.375184687694432	24.396181633726524
24-25	22.61951086460645	26.41244367588004	26.4528998832125	24.515145576301002
26-27	22.68286767350196	26.658133621279944	26.330246573973763	24.32875213124433
28-29	22.55787705314007	26.476890336262464	26.301369237995065	24.6638633726024
30-31	22.624775985459745	26.196920997110162	26.46054999181551	24.717753025614584
32-33	22.84900548511419	26.393547934014755	26.318989764212496	24.438456816658558
34-35	22.815473399666768	26.498306403303335	26.100805174036008	24.58541502299389
36-37	22.563795762831322	26.436977963669882	26.302517476020544	24.696708797478255
38-39	22.891493920184352	26.5400574825712	26.369817113062986	24.19863148418146
40-41	23.066134652559832	26.430273580453484	25.96937160629794	24.53422016068874
42-43	22.800236875712265	26.471838255350065	26.19952463025118	24.528400238686494
44-45	22.928511092751513	26.391261222162054	26.371445204523127	24.308782480563302
46-47	22.909193461315127	26.159806478228536	26.351205348003088	24.57979471245325
48-49	22.729270295674336	26.007650504855828	26.459344435322397	24.803734764147443
50-51	22.984464808881018	25.994259234364403	26.355719735295587	24.665556221458992
52-53	23.214019182790103	26.30584756560793	26.046925511771512	24.433207739830454
54-55	22.74968541792178	26.25044310595768	26.353515819326052	24.64635565679449
56-57	22.878423219752374	26.397292357935875	26.248003449548175	24.47628097276358
58-59	22.940947066276134	26.29501727734851	26.18862650123014	24.575409155145223
60-61	22.94555894766223	26.1754150832549	26.169931574723705	24.709094394359163
62-63	23.041204774751524	26.06043035891726	26.289992192895234	24.608372673435987
64-65	23.083318281615778	26.252839532395168	26.01648004776646	24.6473621382226
66-67	23.15675838158872	26.119432954548436	26.095727344710305	24.628081319152535
68-69	23.121345799800324	26.275065334464554	26.258748252431978	24.34484061330314
70-71	23.437199294806042	26.2015903344419	26.0691675991687	24.292042771583354
72-73	23.25716493867359	26.070543260487977	26.06957462056861	24.602717180269824
74-75	23.29143439740868	26.230144470184896	26.094130266229236	24.38429086617719
76-77	23.28163125339573	26.178254660967028	26.081982720175606	24.45813136546164
78-79	23.08281376786714	26.21502262007951	25.945763478594426	24.756400133458925
80-81	23.278983090833393	26.247967074499247	26.012087592809984	24.460962241857377
82-83	23.31572373811207	26.293414018411003	25.77921485518421	24.611647388292717
84-85	23.205117698592787	26.329684865858777	25.89778069562706	24.567416739921377
86-87	23.180942074468923	26.333411807869094	25.916448974412827	24.569197143249156
88-89	23.359109091398064	26.07304403088062	25.864969086791227	24.702877790930085
90-91	23.249856956288415	26.07420681225574	26.11149728544432	24.564438946011528
92-93	23.51379572715664	26.043559177742353	26.043017672507894	24.399627422593113
94-95	23.352902950376226	26.11696814138005	26.066318940507642	24.46380996773608
96-97	23.35700782838615	25.92215542403782	26.09656395110116	24.624272796474877
98-99	23.504089669242017	25.13163271160718	26.58891729346935	24.775360325681454
100-101	24.406758158626374	11.681387956486418	32.54949631992662	31.36235756496059
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	118437.0
1	59260.0
2	77.5
3	70.5
4	84.0
5	100.5
6	109.5
7	118.0
8	115.0
9	129.5
10	187.0
11	242.5
12	292.0
13	392.5
14	533.5
15	726.5
16	1120.5
17	1700.0
18	2472.0
19	3538.0
20	4698.0
21	6605.5
22	10018.5
23	14670.5
24	20421.5
25	28410.0
26	38194.0
27	53692.0
28	75363.0
29	100874.5
30	139786.5
31	191451.5
32	273586.5
33	384285.5
34	509831.0
35	670912.0
36	906478.5
37	1214137.5
38	1588671.5
39	2096981.0
40	2596095.0
41	3040316.0
42	3555087.0
43	4085942.5
44	4495092.5
45	4790714.0
46	4949798.5
47	4977862.5
48	4869846.5
49	4657117.5
50	4364891.0
51	4039073.0
52	3607063.0
53	3033937.0
54	2559343.0
55	2229519.5
56	1957118.0
57	1661052.0
58	1386499.5
59	1118099.5
60	922044.5
61	819179.5
62	768166.5
63	716947.5
64	607937.5
65	500901.5
66	441831.0
67	398071.5
68	328169.5
69	250310.5
70	185136.5
71	135982.0
72	101783.0
73	79329.0
74	68236.0
75	49469.5
76	28202.0
77	21941.0
78	16210.5
79	11193.5
80	9203.0
81	6055.0
82	2535.5
83	377.5
84	260.5
85	205.5
86	158.5
87	138.0
88	109.5
89	79.5
90	66.0
91	55.0
92	50.0
93	54.0
94	64.0
95	96.5
96	229.0
97	635.0
98	1918.5
99	2314.5
100	929.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.17910132784444047
2	0.15285857387519522
3	0.14466564336400664
4	0.14287963284844074
5	0.14290621757871302
6	0.14287600765794906
7	0.14287600765794906
8	0.14287600765794906
9	0.14287600765794906
10-11	0.14287661185636436
12-13	0.14289715460248384
14-15	0.1429690542139021
16-17	0.1428808412452713
18-19	0.14306995534925376
20-21	0.14291105116603528
22-23	0.14358896178797875
24-25	0.14707579084255615
26-27	0.15001038254556817
28-29	0.15298122615349694
30-31	0.1552753675363128
32-33	0.15732662115618634
34-35	0.15937062439507654
36-37	1.3434835004552168E-4
38-39	2.1181396889885868E-5
40-41	2.8444118747853338E-5
42-43	3.3709932386469547E-4
44-45	7.202078067028095E-5
46-47	1.8762067754414288E-4
48-49	1.4162605105897552E-4
50-51	5.991977359670645E-5
52-53	9.62365836593852E-5
54-55	3.45006056399738E-5
56-57	1.8158574655998666E-6
58-59	6.052978134246E-7
60-61	0.0
62-63	6.658562711587559E-6
64-65	3.6320250173883194E-6
66-67	7.264231291962059E-6
68-69	0.0
70-71	1.8161496128068913E-6
72-73	1.0291798084099452E-5
74-75	0.0
76-77	1.8163031772779167E-6
78-79	0.0
80-81	6.054743344364249E-7
82-83	6.418259167452363E-5
84-85	6.660711385169108E-6
86-87	9.083167314733497E-6
88-89	2.585790785326825E-4
90-91	4.2998334680553925E-5
92-93	1.1508499184132207E-5
94-95	6.051697818967501E-4
96-97	2.729008062150842E-5
98-99	1.9122952081422914E-5
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
34-35	132935.0
36-37	1329.0
38-39	1501.0
40-41	1510.0
42-43	1578.0
44-45	1553.0
46-47	1541.0
48-49	1481.0
50-51	1527.0
52-53	1677.0
54-55	1645.0
56-57	1618.0
58-59	1693.0
60-61	1826.0
62-63	1796.0
64-65	2036.0
66-67	2077.0
68-69	2112.0
70-71	2245.0
72-73	2298.0
74-75	2381.0
76-77	2515.0
78-79	2801.0
80-81	2944.0
82-83	3064.0
84-85	3437.0
86-87	3542.0
88-89	4302.0
90-91	12994.0
92-93	8377.0
94-95	28931.0
96-97	582325.0
98-99	7432650.0
100-101	7.4498031E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.854497176652316
#Duplication Level	Percentage of deduplicated	Percentage of total
1	57.594289252933976	12.58694231869542
2	16.807166916667693	7.346243638556767
3	7.885522703987446	5.170024010121642
4	4.450774117002644	3.8907772149580597
5	2.7244968547373327	2.977125440982758
6	1.8525363762061584	2.429175060206592
7	1.3676930952173154	2.092317142156758
8	1.0027988527094955	1.753253175623188
9	0.8016612872910125	1.5767913905759607
>10	4.762616103720065	19.280947504895234
>50	0.3970571858774422	6.018062983084165
>100	0.28794890187411076	12.507357712694677
>500	0.035159591585276075	5.320538298285318
>1k	0.02764391944196976	11.946188842460502
>5k	0.002134158420700243	3.1639175052427677
>10k+	5.006823274370072E-4	1.9403377614598964
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	118236	0.14287600765794906	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.2083968305587898E-6	0.0	1.2083968305587898E-6	0.0	6.0419841527939486E-6
2	1.2083968305587898E-6	2.4167936611175797E-6	1.2083968305587898E-6	0.0	6.0419841527939486E-6
3	8.458777813911528E-6	2.4167936611175797E-6	1.2083968305587898E-6	0.0	1.2083968305587897E-5
4	1.8125952458381845E-5	2.4167936611175797E-6	8.458777813911528E-6	1.2083968305587898E-6	1.2083968305587897E-5
5	2.7793127102852165E-5	2.4167936611175797E-6	1.2083968305587897E-5	2.4167936611175797E-6	1.4500761966705477E-5
6	3.383511125564611E-5	3.6251904916763693E-6	2.1751142950058216E-5	3.6251904916763693E-6	1.4500761966705477E-5
7	3.50435080862049E-5	3.6251904916763693E-6	3.0209920763969744E-5	3.6251904916763693E-6	1.5709158797264266E-5
8	4.229388906955764E-5	3.6251904916763693E-6	3.0209920763969744E-5	4.833587322235159E-6	1.6917555627823056E-5
9	4.71274763917928E-5	3.6251904916763693E-6	3.0209920763969744E-5	4.833587322235159E-6	2.7793127102852165E-5
10-11	6.94828177571304E-5	4.229388906955764E-6	3.262671442508732E-5	4.833587322235159E-6	3.323091284036672E-5
12-13	8.33793813085565E-5	5.437785737514554E-6	5.377365895986614E-5	4.833587322235159E-6	4.350228590011643E-5
14-15	1.1177670682668805E-4	6.646182568073344E-6	6.887861934185101E-5	4.833587322235159E-6	5.6190452620983725E-5
16-17	1.3473624660730504E-4	8.458777813911528E-6	8.27751828932771E-5	4.833587322235159E-6	6.102403994321888E-5
18-19	1.425908260059372E-4	1.0271373059749713E-5	8.881716704607105E-5	7.250380983352739E-6	6.344083360433647E-5
20-21	1.4682021491289296E-4	1.2083968305587897E-5	1.0331792901277652E-4	8.458777813911528E-6	6.767022251129222E-5
22-23	1.540705958962457E-4	1.3292365136146688E-5	1.2748586562395234E-4	8.458777813911528E-6	7.00870161724098E-5
24-25	1.7280074676990695E-4	1.4500761966705477E-5	1.305068577003493E-4	9.667174644470317E-6	7.492060349464497E-5
26-27	1.9273929447412698E-4	1.4500761966705477E-5	1.3413204819202564E-4	1.2083968305587899E-5	7.492060349464497E-5
28-29	2.2536600889921428E-4	1.4500761966705477E-5	1.4319502442121658E-4	1.631335721254366E-5	7.492060349464497E-5
30-31	2.3986677086591977E-4	1.5709158797264266E-5	1.4923700857401054E-4	1.8125952458381845E-5	7.794159557104194E-5
32-33	2.6645150113821314E-4	1.631335721254366E-5	1.5829998480320145E-4	1.9938547704220032E-5	8.33793813085565E-5
34-35	2.755144773674041E-4	1.6917555627823056E-5	1.7884273092270088E-4	2.0542746119499427E-5	8.760877021551225E-5
36-37	2.954530250716241E-4	1.8125952458381845E-5	1.9213509605884758E-4	2.0542746119499427E-5	9.848434169054137E-5
38-39	3.153915727758441E-4	1.8125952458381845E-5	1.999896754574797E-4	2.0542746119499427E-5	1.063389210891735E-4
40-41	3.323091284036672E-4	1.993854770422003E-5	2.1751142950058217E-4	2.0542746119499427E-5	1.2083968305587897E-4
42-43	3.540602713537254E-4	2.1751142950058216E-5	2.2838700097561128E-4	2.235534136533761E-5	1.2386067513227595E-4
44-45	3.9272896993160667E-4	2.1751142950058216E-5	2.35637381958964E-4	2.7793127102852165E-5	1.2567327037811413E-4
46-47	4.3441866058588493E-4	2.1751142950058216E-5	2.5074234234094886E-4	2.7793127102852165E-5	1.292984608697905E-4
48-49	4.77316748070722E-4	2.1751142950058216E-5	2.6584730272293373E-4	2.7793127102852165E-5	1.413824291753784E-4
50-51	5.081308672499711E-4	2.1751142950058216E-5	2.749102789521247E-4	2.7793127102852165E-5	1.5588319114208388E-4
52-53	5.413617800903378E-4	2.1751142950058216E-5	2.9666142190218293E-4	2.7793127102852165E-5	1.6011258004903963E-4
54-55	5.782178834223809E-4	2.1751142950058216E-5	3.1357897753000595E-4	2.7793127102852165E-5	1.661545642018336E-4
56-57	6.277621534752913E-4	2.1751142950058216E-5	3.298923347425496E-4	2.7793127102852165E-5	1.8428051666021542E-4
58-59	6.73077034621246E-4	2.2959539780617005E-5	3.347259220647848E-4	2.839732551813156E-5	1.9938547704220031E-4
60-61	7.189961141824798E-4	2.2959539780617005E-5	3.6554004124403393E-4	2.9001523933410955E-5	2.0421906436443547E-4
62-63	7.55852217514523E-4	2.2959539780617005E-5	4.0904232714415034E-4	2.9001523933410955E-5	2.0844845327139125E-4
64-65	8.01771297075757E-4	2.2959539780617005E-5	4.301892716789292E-4	2.9001523933410955E-5	2.2174081840753792E-4
66-67	8.392315988230795E-4	2.2959539780617005E-5	4.313976685094879E-4	3.262671442508732E-5	2.2597020731449368E-4
68-69	8.972346466899014E-4	2.4167936611175794E-5	4.3562705741644373E-4	3.262671442508732E-5	2.2778280256033188E-4
70-71	9.528209008956058E-4	2.4167936611175794E-5	4.4106484315395824E-4	3.262671442508732E-5	2.380541756200816E-4
72-73	0.0010289499012208095	2.4167936611175794E-5	4.6402438293457527E-4	3.262671442508732E-5	2.465129534339931E-4
74-75	0.0010851403538417932	2.5376333441734584E-5	4.6402438293457527E-4	3.262671442508732E-5	2.5134654075622826E-4
76-77	0.0011304552349877478	2.6584730272293376E-5	4.658369781804135E-4	3.383511125564611E-5	2.567843264937428E-4
78-79	0.0011757701161337025	2.6584730272293376E-5	4.664411765956928E-4	3.383511125564611E-5	2.640347074770956E-4
80-81	0.001213230417881025	3.0209920763969744E-5	4.676495734262516E-4	3.4439309670925506E-5	2.6524310430765433E-4
82-83	0.0012446487354755534	3.0209920763969744E-5	4.7792094648600134E-4	3.50435080862049E-5	2.712850884604483E-4
84-85	0.0012754628546548027	3.0209920763969744E-5	4.803377401471189E-4	3.50435080862049E-5	2.749102789521247E-4
86-87	0.0012881510213756699	3.0209920763969744E-5	4.948385021138244E-4	3.625190491676369E-5	2.7672287419796285E-4
88-89	0.0012917762118673463	3.0209920763969744E-5	4.978594941902214E-4	3.625190491676369E-5	2.857858504271538E-4
90	0.0012917762118673463	3.0209920763969744E-5	4.990678910207801E-4	3.625190491676369E-5	2.9484882665634473E-4
>>END_MODULE
