##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	SRR21290296_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	75361968
Sequences flagged as poor quality	0
Sequence length	35-101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.04118943656036	37.0	37.0	37.0	37.0	37.0
2	36.06265031454593	37.0	37.0	37.0	37.0	37.0
3	36.15067752742338	37.0	37.0	37.0	37.0	37.0
4	36.123870332048654	37.0	37.0	37.0	37.0	37.0
5	36.091403080131876	37.0	37.0	37.0	37.0	37.0
6	36.007185958838015	37.0	37.0	37.0	37.0	37.0
7	35.96615012760813	37.0	37.0	37.0	37.0	37.0
8	36.046051199193734	37.0	37.0	37.0	37.0	37.0
9	35.98831265924478	37.0	37.0	37.0	37.0	37.0
10-11	36.02418861991502	37.0	37.0	37.0	37.0	37.0
12-13	36.00802218965407	37.0	37.0	37.0	37.0	37.0
14-15	35.99803691432262	37.0	37.0	37.0	37.0	37.0
16-17	35.98066642182168	37.0	37.0	37.0	37.0	37.0
18-19	35.9886971502655	37.0	37.0	37.0	37.0	37.0
20-21	35.98112494886014	37.0	37.0	37.0	37.0	37.0
22-23	35.94960069912187	37.0	37.0	37.0	37.0	37.0
24-25	35.937708732606346	37.0	37.0	37.0	37.0	37.0
26-27	35.90867802178414	37.0	37.0	37.0	37.0	37.0
28-29	35.891647322691995	37.0	37.0	37.0	37.0	37.0
30-31	35.87117433026696	37.0	37.0	37.0	37.0	37.0
32-33	35.847062114938396	37.0	37.0	37.0	37.0	37.0
34-35	35.82992394784594	37.0	37.0	37.0	37.0	37.0
36-37	35.821917764012625	37.0	37.0	37.0	37.0	37.0
38-39	35.807869694678836	37.0	37.0	37.0	37.0	37.0
40-41	35.811395976786514	37.0	37.0	37.0	37.0	37.0
42-43	35.77341241494611	37.0	37.0	37.0	37.0	37.0
44-45	35.76024035785193	37.0	37.0	37.0	37.0	37.0
46-47	35.762699299623236	37.0	37.0	37.0	37.0	37.0
48-49	35.736121853328996	37.0	37.0	37.0	37.0	37.0
50-51	35.715492792011474	37.0	37.0	37.0	37.0	37.0
52-53	35.702445418563556	37.0	37.0	37.0	37.0	37.0
54-55	35.69766924833188	37.0	37.0	37.0	37.0	37.0
56-57	35.66332154953083	37.0	37.0	37.0	37.0	37.0
58-59	35.685115195509255	37.0	37.0	37.0	37.0	37.0
60-61	35.6492206337538	37.0	37.0	37.0	37.0	37.0
62-63	35.6363864706376	37.0	37.0	37.0	37.0	37.0
64-65	35.62579233864665	37.0	37.0	37.0	37.0	37.0
66-67	35.60485182828626	37.0	37.0	37.0	37.0	37.0
68-69	35.56772898471789	37.0	37.0	37.0	37.0	37.0
70-71	35.573325740404755	37.0	37.0	37.0	37.0	37.0
72-73	35.550910939110125	37.0	37.0	37.0	37.0	37.0
74-75	35.56343477546357	37.0	37.0	37.0	37.0	37.0
76-77	35.53779473625892	37.0	37.0	37.0	37.0	37.0
78-79	35.489135703380015	37.0	37.0	37.0	37.0	37.0
80-81	35.48230762707253	37.0	37.0	37.0	37.0	37.0
82-83	35.448176191642034	37.0	37.0	37.0	37.0	37.0
84-85	35.401917872627976	37.0	37.0	37.0	37.0	37.0
86-87	35.360778434695845	37.0	37.0	37.0	37.0	37.0
88-89	35.38665557401262	37.0	37.0	37.0	37.0	37.0
90-91	35.3223285352998	37.0	37.0	37.0	37.0	37.0
92-93	35.364696427092085	37.0	37.0	37.0	37.0	37.0
94-95	35.31379482889163	37.0	37.0	37.0	37.0	37.0
96-97	35.28633198908716	37.0	37.0	37.0	37.0	37.0
98-99	35.27990219863733	37.0	37.0	37.0	37.0	37.0
100-101	35.257865317700876	37.0	37.0	37.0	31.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	23935.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	27.0
12	1049.0
13	7429.0
14	14944.0
15	16823.0
16	15403.0
17	15928.0
18	17502.0
19	22191.0
20	32176.0
21	52579.0
22	81018.0
23	119622.0
24	158836.0
25	200943.0
26	244889.0
27	316190.0
28	406469.0
29	543954.0
30	722875.0
31	1032536.0
32	1509972.0
33	2506660.0
34	4652414.0
35	1.1885705E7
36	4.3144582E7
37	7615317.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.152502404864386	16.151167085463722	12.015158895678253	46.68117161399364
2	22.327174973489626	19.964772933964746	32.253140810781424	25.454911281764208
3	23.609924321449476	20.43004554055663	23.292955856503955	32.66707428148994
4	24.88444413083382	25.571996043833845	20.628504625261147	28.915055200071187
5	26.05662881306842	29.444134285102187	22.991752327721528	21.507484574107863
6	21.57089061834697	33.76572705532564	23.043234301642432	21.62014802468496
7	19.04127911393273	21.902621633560965	38.07143369974369	20.984665552762618
8	19.988409231461375	24.079770535648358	29.449063117151645	26.48275711573862
9	20.91979680222861	22.308801779011187	31.529799338663363	25.241602080096836
10-11	22.85013036580074	30.65104387546207	23.746789966976827	22.75203579176036
12-13	22.13421648549342	25.838427950148624	27.360992560223714	24.666363004134247
14-15	22.221532368384516	26.426252641096752	26.889244633718807	24.462970356799925
16-17	22.98516647041287	26.44053209351348	26.253032250722917	24.32126918535073
18-19	22.805954882330994	26.79185885780132	25.91706236239759	24.485123897470093
20-21	22.91273903344809	26.63143052711041	26.207157870626553	24.248672568814946
22-23	22.77985195465605	26.729104951323574	26.253000536839405	24.238042557180975
24-25	22.656663670321674	26.639661619848436	26.366687969195755	24.336986740634135
26-27	22.779689819538152	26.818663267430182	26.28587059524909	24.115776317782576
28-29	22.665531652807307	26.68980342297333	26.231261399167273	24.413403525052097
30-31	22.696016046720025	26.46413137885623	26.32744058314963	24.512411991274114
32-33	22.883215664930326	26.63086027564658	26.212847337983465	24.273076721439637
34-35	22.887057876559542	26.632993177658413	26.063897826777165	24.416051119004877
36-37	22.690364499714928	26.532896843891756	26.251113377926444	24.525625278466872
38-39	22.957372959741853	26.673878565210572	26.246282153116823	24.122466321930748
40-41	23.091544716631297	26.54351913825696	25.98457066546936	24.380365479642382
42-43	22.909425649102307	26.464958267055977	26.256558764797216	24.369057319044504
44-45	23.01307359516126	26.490934876947136	26.303703464309258	24.192288063582346
46-47	22.99301370229164	26.426730779707757	26.182665336530064	24.39759018147054
48-49	22.830760473868608	26.296502512256136	26.29512818180902	24.577608832066232
50-51	23.052626987215927	26.36942533488591	26.18149258123316	24.396455096664997
52-53	23.28413256447297	26.490035010107842	25.900683476927515	24.32514894849167
54-55	22.913940252494218	26.4128845502226	26.18999230685753	24.483182890425653
56-57	22.996130637205503	26.538628044511846	26.193606862928792	24.27163445535386
58-59	23.054155973290793	26.425423420831347	26.129032740015663	24.391387865862196
60-61	23.05964471980345	26.288749453359507	26.127588786315737	24.524017040521308
62-63	23.169738379346004	26.239901130804537	26.204918182800274	24.385442307049185
64-65	23.21407060894454	26.399753697589308	25.956442164307326	24.429733529158828
66-67	23.21616328848877	26.354944415478478	26.0107718517933	24.418120444239452
68-69	23.234064135080988	26.447575108165072	26.098107198641518	24.22025355811242
70-71	23.476034417255004	26.39585823376046	25.923172332088267	24.204935016896265
72-73	23.23550060673159	26.340276787672344	25.996830402124584	24.42739220347148
74-75	23.255374071282144	26.41821925954443	26.032879851805003	24.29352681736842
76-77	23.35822507215875	26.357381497223987	25.94575901580883	24.338634414808443
78-79	23.162380023301832	26.371323589587597	25.973261242624112	24.493035144486456
80-81	23.345233350461093	26.43132694223418	25.937830686741076	24.28560902056365
82-83	23.423448070434016	26.444478280835774	25.774951556984483	24.357122091745723
84-85	23.31981362194253	26.410537254806364	25.87653679145298	24.393112331798125
86-87	23.344315837614023	26.414486750515543	25.86752602514124	24.373671386729193
88-89	23.51300492953283	26.234920303995533	25.811708031007726	24.440366735463915
90-91	23.365561518213262	26.224293491138795	26.04327971150128	24.36686527914666
92-93	23.55772458171582	26.24686207274533	26.005085846203034	24.19032749933581
94-95	23.526527272340388	26.25267012963804	25.93005459385209	24.290748004169476
96-97	23.409406198022502	26.107958545821074	26.023024899392116	24.45961035676431
98-99	23.59428880050688	25.32214600509251	26.443539621538076	24.640025572862534
100-101	24.467519994171838	11.88245193900463	32.51191728802764	31.13811077879589
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	24015.0
1	12062.0
2	202.0
3	469.5
4	810.0
5	1172.0
6	1475.0
7	1682.0
8	1942.5
9	2174.5
10	2377.0
11	2709.0
12	2969.5
13	3204.0
14	3533.5
15	3962.0
16	4540.5
17	5263.5
18	6260.0
19	7500.5
20	8960.0
21	11128.5
22	14495.0
23	19117.5
24	25246.0
25	33389.0
26	43819.0
27	60157.5
28	82197.5
29	109233.5
30	148621.5
31	201879.5
32	280417.5
33	386527.0
34	517331.0
35	686373.0
36	912051.0
37	1194468.5
38	1545743.5
39	1981253.5
40	2412130.0
41	2830498.5
42	3285912.5
43	3703650.5
44	4046122.0
45	4296815.5
46	4418858.0
47	4430059.5
48	4305699.0
49	4086837.5
50	3817529.5
51	3517789.0
52	3169523.0
53	2747931.5
54	2355794.5
55	2057671.5
56	1799603.5
57	1531676.5
58	1271144.0
59	1034762.5
60	864020.5
61	758003.0
62	691871.5
63	623990.0
64	542604.0
65	473855.5
66	420716.0
67	365598.5
68	297041.5
69	231352.5
70	178799.5
71	136890.5
72	104531.5
73	82565.0
74	69812.5
75	53277.0
76	35296.5
77	28816.5
78	21456.5
79	15472.0
80	14173.0
81	10573.5
82	5428.5
83	2284.5
84	1958.0
85	1754.0
86	1621.0
87	1506.5
88	1399.0
89	1325.0
90	1222.0
91	1116.0
92	1027.5
93	1011.5
94	1002.5
95	984.5
96	1014.0
97	1057.0
98	1191.5
99	1713.5
100	9226.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.07879969376595897
2	0.047500086515787375
3	0.03251640137635471
4	0.03235584293658573
5	0.03230807348343132
6	0.03327142412204522
7	0.03176801327693566
8	0.03241290089452017
9	0.0317627055599185
10-11	0.031790571074258575
12-13	0.03177995564022426
14-15	0.03177730178171568
16-17	0.032604642171764946
18-19	0.03183170588114154
20-21	0.03181445580083578
22-23	0.03404568734192292
24-25	0.03636781353692887
26-27	0.039485433819881136
28-29	0.042334350928839866
30-31	0.04501740188101245
32-33	0.04817947429398341
34-35	0.050586523961263855
36-37	2.0578269144655813E-4
38-39	5.025202285634596E-4
40-41	6.545557608717148E-4
42-43	0.001083431458454752
44-45	8.683628563869376E-4
46-47	6.33364864369628E-4
48-49	5.855808483990936E-4
50-51	0.0010032168603427386
52-53	3.6650626219106395E-4
54-55	2.3903288569290707E-5
56-57	8.034412304220379E-5
58-59	1.9256622139867035E-5
60-61	5.445152578421187E-5
62-63	3.984390274533654E-6
64-65	1.527403038296842E-5
66-67	6.64111647634367E-6
68-69	2.1252360282151913E-5
70-71	2.1253200972825426E-5
72-73	0.0
74-75	8.634832743920757E-6
76-77	4.118335426477314E-5
78-79	5.712793251793528E-5
80-81	2.7037468743407335E-4
82-83	0.001602401093071395
84-85	1.6808839775481736E-4
86-87	0.0012670503147075253
88-89	5.515036391233412E-4
90-91	0.0023085467337476033
92-93	9.863355570990716E-4
94-95	0.0011167619932709904
96-97	0.0017962340273183434
98-99	0.001738390241697057
100-101	3.674912697217231E-4
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
34-35	39356.0
36-37	1797.0
38-39	2085.0
40-41	1996.0
42-43	2080.0
44-45	2126.0
46-47	2154.0
48-49	2104.0
50-51	2096.0
52-53	2156.0
54-55	2376.0
56-57	2252.0
58-59	2450.0
60-61	2428.0
62-63	2653.0
64-65	2615.0
66-67	2813.0
68-69	2887.0
70-71	3194.0
72-73	3018.0
74-75	3359.0
76-77	3414.0
78-79	3664.0
80-81	3879.0
82-83	3892.0
84-85	4468.0
86-87	4507.0
88-89	5705.0
90-91	13669.0
92-93	9718.0
94-95	29287.0
96-97	542132.0
98-99	6774735.0
100-101	6.7874903E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.53113611691127
#Duplication Level	Percentage of deduplicated	Percentage of total
1	64.34093769213258	18.357200511839412
2	14.615124441361568	8.339722096041674
3	6.694678143061898	5.730203200758294
4	3.554692442859206	4.056776557638873
5	2.2803076556727127	3.2529884056216507
6	1.5438568640064314	2.6428794199197156
7	1.089936022380586	2.1767979109285744
8	0.8429898324720835	1.9241166124346596
9	0.6329392837638435	1.625262917292589
>10	3.8219408032616116	20.26798966435205
>50	0.3226435765170882	6.39861428774193
>100	0.22225961442020936	12.395509900054385
>500	0.0238085911573898	4.687023359342252
>1k	0.013352505663647856	6.894914097873873
>5k	4.270795326306017E-4	0.7931784853823395
>10k+	1.054517364520004E-4	0.45682257277763283
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.326929254289113E-6	0.0	1.326929254289113E-6	0.0	6.634646271445565E-6
2	1.326929254289113E-6	2.653858508578226E-6	6.634646271445565E-6	0.0	7.961575525734678E-6
3	3.980787762867339E-6	2.653858508578226E-6	7.961575525734678E-6	0.0	1.7250080305758468E-5
4	9.288504780023792E-6	2.653858508578226E-6	1.0615434034312905E-5	0.0	2.123086806862581E-5
5	1.326929254289113E-5	2.653858508578226E-6	1.4596221797180244E-5	0.0	2.3884726577204035E-5
6	1.9903938814336694E-5	2.653858508578226E-6	2.255779732291492E-5	0.0	2.5211655831493147E-5
7	2.255779732291492E-5	2.653858508578226E-6	3.4500160611516937E-5	0.0	2.7865514340071373E-5
8	2.9192443594360488E-5	2.653858508578226E-6	3.4500160611516937E-5	2.653858508578226E-6	2.9192443594360488E-5
9	3.05193728486496E-5	2.653858508578226E-6	3.4500160611516937E-5	3.980787762867339E-6	3.3173231357227825E-5
10-11	4.3125200764396174E-5	5.307717017156452E-6	3.9807877628673396E-5	3.980787762867339E-6	3.582708986580605E-5
12-13	5.7057957934431864E-5	7.961575525734678E-6	5.63944933072873E-5	3.980787762867339E-6	4.511559464582984E-5
14-15	6.966378585017844E-5	9.951969407168349E-6	7.563496749447944E-5	3.980787762867339E-6	5.04233116629863E-5
16-17	8.824079541022602E-5	1.127889866145746E-5	9.62023709359607E-5	3.980787762867339E-6	5.175024091727541E-5
18-19	9.819276481739436E-5	1.4596221797180244E-5	1.068178049702736E-4	3.980787762867339E-6	5.63944933072873E-5
20-21	1.0283701720740625E-4	1.4596221797180244E-5	1.2473134990317663E-4	4.644252390011896E-6	7.0327250477323E-5
22-23	1.1544284512315283E-4	1.52596864243248E-5	1.4662568259894698E-4	5.307717017156452E-6	7.497150286733489E-5
24-25	1.4397182409036876E-4	1.8577009560047583E-5	1.4994300573466978E-4	7.298110898590122E-6	7.696189674876855E-5
26-27	1.771450554475966E-4	2.0567403441481254E-5	1.52596864243248E-4	7.961575525734678E-6	8.09426845116359E-5
28-29	2.1761639770341454E-4	2.5211655831493147E-5	1.652026921589946E-4	8.625040152879234E-6	8.824079541022602E-5
30-31	2.733474263835573E-4	2.5211655831493147E-5	1.7316426768472927E-4	9.288504780023792E-6	9.15581185459488E-5
32-33	3.1846302102938717E-4	2.7202049712926817E-5	1.8046237858331936E-4	1.0615434034312905E-5	9.553890630881614E-5
34-35	3.602612925394942E-4	2.852897896721593E-5	2.1429907456769175E-4	1.127889866145746E-5	1.0283701720740625E-4
36-37	4.2594429062680527E-4	3.05193728486496E-5	2.2226065009342641E-4	1.1942363288602018E-5	1.1212552198743006E-4
38-39	5.121946921555977E-4	3.05193728486496E-5	2.322126195005948E-4	1.1942363288602018E-5	1.2406788527603206E-4
40-41	5.74560367107186E-4	3.3173231357227825E-5	2.514530936877869E-4	1.326929254289113E-5	1.3667371319177863E-4
42-43	6.34272183550196E-4	3.7154019120095166E-5	2.720204971292682E-4	1.4596221797180244E-5	1.3932757170035686E-4
44-45	7.424169177747588E-4	3.848094837438428E-5	2.779916787735692E-4	1.6586615678613913E-5	1.4994300573466978E-4
46-47	8.253499961678283E-4	4.047134225581795E-5	3.0718412236792965E-4	1.924047418719214E-5	1.559141873789708E-4
48-49	9.202254378494999E-4	4.511559464582984E-5	3.1978995028367625E-4	2.653858508578226E-5	1.6387576290470546E-4
50-51	0.001008466233259726	4.511559464582984E-5	3.3040538431798914E-4	2.653858508578226E-5	1.725008030575847E-4
52-53	0.0010880819885170727	4.511559464582984E-5	3.542901108951932E-4	2.653858508578226E-5	1.797989139561748E-4
54-55	0.0011842843594530334	4.511559464582984E-5	3.7220365582809624E-4	2.7202049712926817E-5	1.890874187361986E-4
56-57	0.0013063618508476317	5.0423311662986293E-5	3.954249177781557E-4	2.7865514340071373E-5	2.0633749904195708E-4
58-59	0.0014105257973093272	5.307717017156452E-5	4.146653919653478E-4	2.852897896721593E-5	2.2292411472057098E-4
60-61	0.0015312763594496365	5.5731028680142746E-5	4.511559464582984E-4	2.9192443594360488E-5	2.275683671105829E-4
62-63	0.001647382669199934	5.5731028680142746E-5	4.883099655783936E-4	3.05193728486496E-5	2.3420301338202844E-4
64-65	0.0017522100802887738	5.5731028680142746E-5	5.16175479918465E-4	3.250976673008327E-5	2.487992351792087E-4
66-67	0.0018696433192933602	5.5731028680142746E-5	5.16175479918465E-4	3.516362523866149E-5	2.640589216035335E-4
68-69	0.001991720810687959	5.5731028680142746E-5	5.287813078342115E-4	3.781748374723972E-5	2.760012848921355E-4
70-71	0.0021005290095396662	5.5731028680142746E-5	5.301082370885006E-4	3.848094837438428E-5	2.985590822150504E-4
72-73	0.0022445008336300348	5.7057957934431864E-5	5.413871357499581E-4	4.113480688296251E-5	3.1448223326651975E-4
74-75	0.002410366990416174	5.772142256157642E-5	5.413871357499581E-4	4.113480688296251E-5	3.237707380465436E-4
76-77	0.0025423964512179403	6.501953346016654E-5	5.473583173942592E-4	4.113480688296251E-5	3.2974191969084457E-4
78-79	0.0026339545697638893	7.099071510446755E-5	5.513391051571265E-4	4.113480688296251E-5	3.310688489451337E-4
80-81	0.002721531900546971	7.165417973161211E-5	5.559833575471384E-4	4.113480688296251E-5	3.476554646237476E-4
82-83	0.002801811120431462	7.165417973161211E-5	5.626180038185839E-4	4.113480688296251E-5	3.6357861567521695E-4
84-85	0.0029165905009274707	7.298110898590122E-5	5.672622562085958E-4	4.113480688296251E-5	3.7353058508238533E-4
86-87	0.002962369560200445	7.430803824019033E-5	5.705795793443186E-4	4.113480688296251E-5	3.821556252352645E-4
88-89	0.002973648458861902	7.563496749447944E-5	5.705795793443186E-4	4.378866539154073E-5	3.9940570554102304E-4
90	0.002973648458861902	7.563496749447944E-5	5.785411548700533E-4	4.511559464582984E-5	4.086942103210468E-4
>>END_MODULE
