##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	SRR21290311_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	107618968
Sequences flagged as poor quality	0
Sequence length	35-101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.381646941643226	37.0	37.0	37.0	37.0	37.0
2	36.38528912486877	37.0	37.0	37.0	37.0	37.0
3	36.44544737689735	37.0	37.0	37.0	37.0	37.0
4	36.41619247826275	37.0	37.0	37.0	37.0	37.0
5	36.39569729009109	37.0	37.0	37.0	37.0	37.0
6	36.32366492308308	37.0	37.0	37.0	37.0	37.0
7	36.30167708911686	37.0	37.0	37.0	37.0	37.0
8	36.37273629124561	37.0	37.0	37.0	37.0	37.0
9	36.32902522350893	37.0	37.0	37.0	37.0	37.0
10-11	36.35284082077427	37.0	37.0	37.0	37.0	37.0
12-13	36.34073356845421	37.0	37.0	37.0	37.0	37.0
14-15	36.33263719830504	37.0	37.0	37.0	37.0	37.0
16-17	36.319174125512895	37.0	37.0	37.0	37.0	37.0
18-19	36.32398384455796	37.0	37.0	37.0	37.0	37.0
20-21	36.3189041312866	37.0	37.0	37.0	37.0	37.0
22-23	36.291372711360694	37.0	37.0	37.0	37.0	37.0
24-25	36.279574154623006	37.0	37.0	37.0	37.0	37.0
26-27	36.25742386788173	37.0	37.0	37.0	37.0	37.0
28-29	36.24479137358017	37.0	37.0	37.0	37.0	37.0
30-31	36.22768763216536	37.0	37.0	37.0	37.0	37.0
32-33	36.20833550457388	37.0	37.0	37.0	37.0	37.0
34-35	36.19648457788594	37.0	37.0	37.0	37.0	37.0
36-37	36.179363345305205	37.0	37.0	37.0	37.0	37.0
38-39	36.16658840064851	37.0	37.0	37.0	37.0	37.0
40-41	36.16793495556562	37.0	37.0	37.0	37.0	37.0
42-43	36.13768317369595	37.0	37.0	37.0	37.0	37.0
44-45	36.12892443039554	37.0	37.0	37.0	37.0	37.0
46-47	36.129358831736155	37.0	37.0	37.0	37.0	37.0
48-49	36.106862536221	37.0	37.0	37.0	37.0	37.0
50-51	36.09083831079831	37.0	37.0	37.0	37.0	37.0
52-53	36.080453721593244	37.0	37.0	37.0	37.0	37.0
54-55	36.07373471633768	37.0	37.0	37.0	37.0	37.0
56-57	36.04621162618554	37.0	37.0	37.0	37.0	37.0
58-59	36.06439936092931	37.0	37.0	37.0	37.0	37.0
60-61	36.036436001842596	37.0	37.0	37.0	37.0	37.0
62-63	36.026599703674634	37.0	37.0	37.0	37.0	37.0
64-65	36.01543077278146	37.0	37.0	37.0	37.0	37.0
66-67	35.998189740491355	37.0	37.0	37.0	37.0	37.0
68-69	35.97121624992373	37.0	37.0	37.0	37.0	37.0
70-71	35.972972564081985	37.0	37.0	37.0	37.0	37.0
72-73	35.95341094567694	37.0	37.0	37.0	37.0	37.0
74-75	35.96185102079683	37.0	37.0	37.0	37.0	37.0
76-77	35.940667498477865	37.0	37.0	37.0	37.0	37.0
78-79	35.90250887802076	37.0	37.0	37.0	37.0	37.0
80-81	35.90050628671139	37.0	37.0	37.0	37.0	37.0
82-83	35.87193946972578	37.0	37.0	37.0	37.0	37.0
84-85	35.83272498913954	37.0	37.0	37.0	37.0	37.0
86-87	35.798636180729375	37.0	37.0	37.0	37.0	37.0
88-89	35.81761122208624	37.0	37.0	37.0	37.0	37.0
90-91	35.7666349951682	37.0	37.0	37.0	37.0	37.0
92-93	35.79906065060675	37.0	37.0	37.0	37.0	37.0
94-95	35.755910812893745	37.0	37.0	37.0	37.0	37.0
96-97	35.730332994502895	37.0	37.0	37.0	37.0	37.0
98-99	35.725993874712856	37.0	37.0	37.0	37.0	37.0
100-101	35.69425442717501	37.0	37.0	37.0	37.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	6185.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	16.0
12	478.0
13	3198.0
14	7901.0
15	12086.0
16	15981.0
17	21321.0
18	24880.0
19	29962.0
20	37769.0
21	51520.0
22	70511.0
23	97283.0
24	125714.0
25	160090.0
26	200307.0
27	264721.0
28	346126.0
29	472133.0
30	637110.0
31	921706.0
32	1356399.0
33	2274837.0
34	4352381.0
35	1.2319089E7
36	6.5997624E7
37	1.781164E7
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.164752897336477	15.964673470929164	16.329763285282674	47.540810346451686
2	22.655472277423154	20.889980600173207	31.88246668276271	24.572080439640928
3	24.357516711690636	21.76467810826536	23.05925467770803	30.818550502335974
4	24.41725407493827	26.148198491506363	20.824209941926522	28.610337491628847
5	26.269298099344766	30.264956507885838	23.21776168921889	20.247983703550506
6	21.140667417338648	34.793170435636775	22.56071279015084	21.505449356873736
7	18.895729516846814	20.972975650240006	39.62717805829076	20.504116774622418
8	20.25748993962563	24.02987420116868	29.17652464734688	26.536111211858817
9	20.988465505569742	22.441133129737313	31.463493754770518	25.106907609922423
10-11	23.08682331948256	30.872020385540193	23.364902827311873	22.676253467665376
12-13	22.083863107119598	25.87912882631803	27.487118046554983	24.549890020007386
14-15	22.297111106687844	26.518698998964492	26.951861361875967	24.232328532471694
16-17	23.017320966923776	26.58587276363929	26.31505867946697	24.08174758996996
18-19	22.806216351960774	27.012730663185593	26.04175602066734	24.1392969641863
20-21	23.02770848182011	26.966514086625192	26.20563408694746	23.800143344607235
22-23	22.75403990877308	27.00728258724813	26.48431235429318	23.7543651496856
24-25	22.556790992287745	27.002512126758667	26.579036436292263	23.86166044466132
26-27	22.787610469386895	27.074060807803534	26.43518936477457	23.703139358034996
28-29	22.73366282866707	26.959280972071483	26.324787495526536	23.982268703734906
30-31	22.637102191222986	26.975234357037053	26.399966140276014	23.987697311463943
32-33	22.929223787935705	27.042987618447224	26.25986597297143	23.76792262064564
34-35	22.90341116721408	26.897543382780377	26.225752646553996	23.97329280345155
36-37	22.719605381503644	26.85047491200068	26.380980870487235	24.04893883600844
38-39	23.065537805571772	26.981567412463004	26.232359160086595	23.72053562187863
40-41	23.095138938767413	26.809484547221302	26.14287844031683	23.952498073694457
42-43	22.858088981994793	26.7872835429556	26.45018583814825	23.904441636901357
44-45	23.024376599685137	26.80351740532337	26.420973102993855	23.75113289199764
46-47	22.993710688901285	26.748512943931296	26.341831499159074	23.915944868008346
48-49	22.833282617949006	26.71015275616889	26.364831339480084	24.09173328640203
50-51	23.113760536714555	26.825954714163565	26.190496870234725	23.86978787888716
52-53	23.261479921752017	26.82305162653546	26.070568047004112	23.844900404708415
54-55	22.855161817883936	26.774126195918612	26.394071798108588	23.97664018808887
56-57	23.036626496043993	26.884922532599443	26.336093837896417	23.74235713346015
58-59	23.119804301846987	26.694299908713347	26.30757459498849	23.87832119445117
60-61	23.046560624691054	26.642751134202573	26.31183539768892	23.998852843417453
62-63	23.163042632275992	26.680366607543725	26.364184058634883	23.792406701545403
64-65	23.184027997097704	26.735523478956186	26.255946387729367	23.82450213621674
66-67	23.120288870690175	26.688603940539075	26.358064449692854	23.8330427390779
68-69	23.315695552985492	26.787433577110814	26.204243149113875	23.69262772078982
70-71	23.389396928314106	26.736909956401096	26.107622774550293	23.76607034073451
72-73	23.140434510428605	26.773679823935804	26.25060689462792	23.835278771007676
74-75	23.21418656121367	26.78779198421329	26.321233678859763	23.676787775713283
76-77	23.28193936375213	26.661609894157856	26.24927521128255	23.80717553080746
78-79	23.0995515097763	26.699969758262636	26.342111756672885	23.858366975288178
80-81	23.368799118832627	26.79711028251412	26.182043366687108	23.652047231966147
82-83	23.373070549192658	26.773234692703323	26.10160389926316	23.75209085884086
84-85	23.19820584865264	26.767728224973858	26.23246240616498	23.801603520208516
86-87	23.330428768124868	26.72551540571747	26.262755940617726	23.681299885539932
88-89	23.467369193525034	26.52955856210774	26.217243110523995	23.78582913384323
90-91	23.2472107691774	26.601750011481734	26.362244295402178	23.78879492393869
92-93	23.405272720942204	26.7013674428498	26.264837336002135	23.628522500205857
94-95	23.43669795618796	26.616963186151178	26.24964045609036	23.696698401570504
96-97	23.304321660495305	26.498213914358125	26.393714874764974	23.803749550381596
98-99	23.541522589214367	25.66013791120153	26.811284246273964	23.98705525331014
100-101	24.265856458209665	12.105499655208323	33.21606537269413	30.412578513887883
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	6385.0
1	3279.5
2	243.5
3	439.5
4	736.0
5	1040.5
6	1251.5
7	1396.5
8	1529.0
9	1629.5
10	1735.5
11	1845.0
12	2041.0
13	2278.0
14	2514.0
15	2960.0
16	3564.5
17	4273.0
18	5257.5
19	6710.0
20	8636.5
21	11025.0
22	14816.0
23	21213.0
24	29364.5
25	40062.5
26	56514.5
27	81547.0
28	116534.0
29	159408.5
30	220795.5
31	306175.5
32	428555.5
33	588438.5
34	793821.0
35	1070160.5
36	1423598.5
37	1864901.0
38	2417248.0
39	3060715.5
40	3713726.5
41	4366164.0
42	5034417.0
43	5615755.0
44	6074084.5
45	6381075.5
46	6515480.5
47	6507584.0
48	6270353.0
49	5857633.0
50	5373143.5
51	4847149.5
52	4326816.5
53	3789231.0
54	3256893.0
55	2807118.0
56	2408490.5
57	1997739.0
58	1600764.5
59	1290110.5
60	1084249.0
61	940395.5
62	828924.0
63	723415.0
64	623076.0
65	543858.5
66	480419.0
67	416556.0
68	334873.0
69	252212.5
70	195410.5
71	153108.0
72	114806.0
73	87141.0
74	69459.0
75	52703.0
76	33552.5
77	22224.5
78	16401.0
79	12462.0
80	9786.5
81	6955.0
82	4306.0
83	2445.0
84	2213.5
85	2025.0
86	1846.5
87	1743.0
88	1629.5
89	1543.0
90	1525.0
91	1487.5
92	1421.5
93	1406.0
94	1436.5
95	1379.5
96	1238.5
97	1083.0
98	972.0
99	1081.0
100	6108.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.053674552984005565
2	0.021522228312020237
3	0.006527659696569475
4	0.006362261344115472
5	0.006333456013070113
6	0.007274739895294295
7	0.005763853821753801
8	0.006429164048478889
9	0.005750844962572025
10-11	0.0057796502936173855
12-13	0.005773145864026497
14-15	0.005762924617526532
16-17	0.006629872161569139
18-19	0.005827039709208138
20-21	0.005825645902867234
22-23	0.007403434680771145
24-25	0.006838943112704816
26-27	0.007687306572202031
28-29	0.008688988729198741
30-31	0.009565228315514046
32-33	0.011026866565009247
34-35	0.01186175656321105
36-37	2.327951306618895E-4
38-39	5.90127339675424E-4
40-41	7.039802074408023E-4
42-43	0.0011686686704478466
44-45	9.609705438178776E-4
46-47	6.505700910856214E-4
48-49	6.361746410824892E-4
50-51	0.001087882877962414
52-53	3.829274047248502E-4
54-55	3.438980359053715E-5
56-57	1.1293112555120673E-4
58-59	2.4631617086993668E-5
60-61	5.484159110978768E-5
62-63	4.647713193113933E-6
64-65	1.9056138225901698E-5
66-67	1.0225562760709147E-5
68-69	2.8818501805388496E-5
70-71	2.6960385752546154E-5
72-73	4.6485743563896566E-7
74-75	7.903023872194678E-6
76-77	3.672816283028376E-5
78-79	6.416304719275346E-5
80-81	2.8411173345375577E-4
82-83	0.0016351207720850544
84-85	1.8744468695662843E-4
86-87	0.0013635316722658071
88-89	5.93273506857381E-4
90-91	0.0023523796477493467
92-93	0.0010579828379071803
94-95	0.0011353398896657408
96-97	0.0019185839029261494
98-99	0.00175716724373712
100-101	3.677044564094555E-4
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
34-35	13303.0
36-37	1382.0
38-39	1494.0
40-41	1374.0
42-43	1488.0
44-45	1599.0
46-47	1670.0
48-49	1818.0
50-51	2064.0
52-53	2220.0
54-55	2289.0
56-57	2349.0
58-59	2657.0
60-61	2779.0
62-63	2826.0
64-65	3256.0
66-67	3636.0
68-69	4290.0
70-71	5249.0
72-73	5820.0
74-75	6652.0
76-77	7931.0
78-79	9672.0
80-81	12560.0
82-83	15657.0
84-85	19233.0
86-87	22561.0
88-89	28390.0
90-91	46622.0
92-93	46799.0
94-95	79766.0
96-97	830393.0
98-99	9961240.0
100-101	9.6467929E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.590908734391917
#Duplication Level	Percentage of deduplicated	Percentage of total
1	50.96419133223256	11.513273951085534
2	16.81263184349518	7.596252631226619
3	9.379259178716664	6.356579643077877
4	5.56609669063848	5.029727293800591
5	3.6606295171553906	4.13484736662393
6	2.4955309309065985	3.3825786902377843
7	1.7890748252889455	2.8291778267970513
8	1.3436251367772618	2.428297027053598
9	1.0268086523748543	2.0876886498135865
>10	6.164839492224516	25.74058795544549
>50	0.4758909000193339	7.411033221868732
>100	0.2855683904716495	12.196919573794922
>500	0.022978281151033664	3.542967288202123
>1k	0.012460031133465645	4.976213320658294
>5k	3.1833289906147197E-4	0.4704349860440558
>10k+	9.646451486711272E-5	0.303420574269895
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	9.292042272696761E-7	0.0	4.646021136348381E-6	0.0	5.5752253636180564E-6
2	4.646021136348381E-6	3.7168169090787046E-6	6.504429590887733E-6	0.0	1.672567609085417E-5
3	6.504429590887733E-6	3.7168169090787046E-6	6.504429590887733E-6	0.0	2.6946922590820607E-5
4	1.3938063409045141E-5	3.7168169090787046E-6	1.207965495450579E-5	1.8584084545393523E-6	3.4380556408978014E-5
5	2.32301056817419E-5	3.7168169090787046E-6	2.32301056817419E-5	4.646021136348381E-6	3.530976063624769E-5
6	2.6946922590820607E-5	3.7168169090787046E-6	4.553100713621413E-5	8.362838045427085E-6	3.530976063624769E-5
7	2.9734535272629637E-5	3.7168169090787046E-6	5.946907054525927E-5	8.362838045427085E-6	3.530976063624769E-5
8	3.066373949989931E-5	3.7168169090787046E-6	6.504429590887733E-5	9.292042272696762E-6	3.90265775453264E-5
9	3.159294372716899E-5	3.7168169090787046E-6	6.96903170452257E-5	1.0221246499966437E-5	4.1814190227135425E-5
10-11	3.67035669771522E-5	5.5752253636180564E-6	8.641599313607988E-5	1.0221246499966437E-5	5.25000388407367E-5
12-13	4.9712426158927674E-5	5.5752253636180564E-6	1.110399051587263E-4	1.0221246499966437E-5	7.43363381815741E-5
14-15	6.318588745433798E-5	5.5752253636180564E-6	1.333408066131985E-4	1.0221246499966437E-5	8.641599313607988E-5
16-17	8.130536988609665E-5	5.5752253636180564E-6	1.681859651358114E-4	1.207965495450579E-5	9.059741215879341E-5
18-19	9.059741215879341E-5	7.433633818157409E-6	1.760842010676036E-4	1.207965495450579E-5	9.431422906787212E-5
20-21	9.942485231785535E-5	7.898235931792247E-6	1.9234527504482295E-4	1.207965495450579E-5	9.710184174968115E-5
22-23	1.0592928190874308E-4	9.292042272696762E-6	2.127877680447558E-4	1.3473461295410305E-5	1.0035405654512502E-4
24-25	1.245133664541366E-4	1.0221246499966437E-5	2.1557538072656485E-4	1.5331869749949656E-5	1.0314166922693405E-4
26-27	1.6539835245400235E-4	1.3938063409045143E-5	2.2904884202197518E-4	1.8119482431758686E-5	1.0546467979510824E-4
28-29	2.072125426811378E-4	1.4867267636314818E-5	2.508851413628126E-4	2.0442492999932875E-5	1.1661513052234435E-4
30-31	2.532081519309867E-4	1.5796471863584494E-5	2.629647963173183E-4	2.0442492999932875E-5	1.268363770223108E-4
32-33	2.9409313793085244E-4	1.7654880318123848E-5	2.745798491581893E-4	2.137169722720255E-5	1.333408066131985E-4
34-35	3.224338668625776E-4	1.95132887726632E-5	3.373011344988924E-4	2.137169722720255E-5	1.454204615677043E-4
36-37	3.856197543169156E-4	2.0442492999932875E-5	3.64248057089713E-4	2.137169722720255E-5	1.621461376585585E-4
38-39	4.432304164076355E-4	2.0442492999932875E-5	3.7214629302150524E-4	2.137169722720255E-5	1.7283198627215975E-4
40-41	4.8225699395296195E-4	2.137169722720255E-5	3.9909321561232584E-4	2.137169722720255E-5	1.8351783488576101E-4
42-43	5.42190666611856E-4	2.4159309909011577E-5	4.209295149531633E-4	2.1836299340837388E-5	1.8769925390847456E-4
44-45	5.942261033389578E-4	2.834072893172512E-5	4.237171276349723E-4	2.415930990901158E-5	1.9373908138572749E-4
46-47	6.471907442933294E-4	2.8805331045359958E-5	4.47411835430349E-4	2.415930990901158E-5	2.113939617038513E-4
48-49	7.377881564521228E-4	2.9734535272629637E-5	4.711065432257258E-4	2.5088514136281256E-5	2.2997804624924484E-4
50-51	8.08872279838253E-4	2.9734535272629637E-5	4.831861981802316E-4	2.7876126818090282E-5	2.513497434764474E-4
52-53	8.86460832815271E-4	2.9734535272629637E-5	5.008410784983554E-4	2.7876126818090282E-5	2.546019582718912E-4
54-55	9.728768259513508E-4	2.9734535272629637E-5	5.342924306800638E-4	2.7876126818090282E-5	2.573895709537003E-4
56-57	0.001052788389496543	3.623896486351737E-5	5.691375892026766E-4	2.7876126818090282E-5	2.601771836355093E-4
58-59	0.0011498902312462242	3.809737331805672E-5	5.835402547253565E-4	2.7876126818090282E-5	2.66681613226397E-4
60-61	0.0012428106539731918	3.809737331805672E-5	6.648456246114533E-4	2.834072893172512E-5	2.685400216809364E-4
62-63	0.0013417709041774121	3.9955781772596074E-5	7.308191247476002E-4	3.066373949989931E-5	2.694692259082061E-4
64-65	0.001445841777631616	4.042038388623091E-5	7.712395086338312E-4	3.066373949989931E-5	2.764382576127286E-4
66-67	0.0015712843483130221	4.134958811350059E-5	7.786731424519886E-4	3.159294372716899E-5	2.83871891430886E-4
68-69	0.0016851118661535577	4.1814190227135425E-5	7.935404100883034E-4	3.159294372716899E-5	2.917701273626783E-4
70-71	0.0017942933628577445	4.3672598681674776E-5	7.97721829111017E-4	3.159294372716899E-5	2.996683632944706E-4
72-73	0.0019085854828119148	4.3672598681674776E-5	8.130536988609666E-4	3.159294372716899E-5	3.080312013398976E-4
74-75	0.002045643106334192	4.3672598681674776E-5	8.200227305654892E-4	3.159294372716899E-5	3.131418245898808E-4
76-77	0.0021246254656521143	4.506640502257929E-5	8.339607939745342E-4	3.159294372716899E-5	3.3126130702163957E-4
78-79	0.002206395437651846	4.692481347711864E-5	8.400006214517872E-4	3.159294372716899E-5	3.451993704306847E-4
80-81	0.00226632911031074	5.017702827256251E-5	8.418590299063266E-4	3.2057545840803825E-5	3.619250465215388E-4
82-83	0.0023313734062196173	5.203543672710186E-5	8.511510721790233E-4	3.252214795443866E-5	3.707524866806008E-4
84-85	0.0024307982585374723	5.389384518164121E-5	8.599785123380852E-4	3.252214795443866E-5	3.777215183851233E-4
86-87	0.0024916611354236368	5.482304940891089E-5	8.69270554610782E-4	3.4380556408978014E-5	3.8469055008964596E-4
88-89	0.0025069930051735863	5.482304940891089E-5	8.72058167292591E-4	3.530976063624769E-5	3.976994092714214E-4
90	0.0025069930051735863	5.482304940891089E-5	8.725227694062258E-4	3.530976063624769E-5	4.134958811350059E-4
>>END_MODULE
