##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	SRR21290300_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	85597419
Sequences flagged as poor quality	0
Sequence length	35-101
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.11482220042172	37.0	37.0	37.0	37.0	37.0
2	36.108651114819246	37.0	37.0	37.0	37.0	37.0
3	36.19431855766586	37.0	37.0	37.0	37.0	37.0
4	36.174360701226284	37.0	37.0	37.0	37.0	37.0
5	36.144566894008804	37.0	37.0	37.0	37.0	37.0
6	36.06475343608199	37.0	37.0	37.0	37.0	37.0
7	36.02523799228105	37.0	37.0	37.0	37.0	37.0
8	36.09333446140473	37.0	37.0	37.0	37.0	37.0
9	36.03725806265257	37.0	37.0	37.0	37.0	37.0
10-11	36.06965607806469	37.0	37.0	37.0	37.0	37.0
12-13	36.05352298648164	37.0	37.0	37.0	37.0	37.0
14-15	36.04363636244686	37.0	37.0	37.0	37.0	37.0
16-17	36.02520514666453	37.0	37.0	37.0	37.0	37.0
18-19	36.03242281756182	37.0	37.0	37.0	37.0	37.0
20-21	36.023221196657815	37.0	37.0	37.0	37.0	37.0
22-23	35.989877399223914	37.0	37.0	37.0	37.0	37.0
24-25	35.97784544765304	37.0	37.0	37.0	37.0	37.0
26-27	35.94697391518312	37.0	37.0	37.0	37.0	37.0
28-29	35.92931430561008	37.0	37.0	37.0	37.0	37.0
30-31	35.90927919216817	37.0	37.0	37.0	37.0	37.0
32-33	35.88332332193335	37.0	37.0	37.0	37.0	37.0
34-35	35.86794181259134	37.0	37.0	37.0	37.0	37.0
36-37	35.84540561114291	37.0	37.0	37.0	37.0	37.0
38-39	35.82880273704447	37.0	37.0	37.0	37.0	37.0
40-41	35.83023251475024	37.0	37.0	37.0	37.0	37.0
42-43	35.7930584045494	37.0	37.0	37.0	37.0	37.0
44-45	35.78215935438063	37.0	37.0	37.0	37.0	37.0
46-47	35.78637488298074	37.0	37.0	37.0	37.0	37.0
48-49	35.75684413049581	37.0	37.0	37.0	37.0	37.0
50-51	35.73417494047506	37.0	37.0	37.0	37.0	37.0
52-53	35.7198278245876	37.0	37.0	37.0	37.0	37.0
54-55	35.71514151170849	37.0	37.0	37.0	37.0	37.0
56-57	35.68238864873315	37.0	37.0	37.0	37.0	37.0
58-59	35.70489030393217	37.0	37.0	37.0	37.0	37.0
60-61	35.66754000472859	37.0	37.0	37.0	37.0	37.0
62-63	35.65504268883995	37.0	37.0	37.0	37.0	37.0
64-65	35.64289272319911	37.0	37.0	37.0	37.0	37.0
66-67	35.62110730867869	37.0	37.0	37.0	37.0	37.0
68-69	35.585805668794265	37.0	37.0	37.0	37.0	37.0
70-71	35.587768599345345	37.0	37.0	37.0	37.0	37.0
72-73	35.56478071561142	37.0	37.0	37.0	37.0	37.0
74-75	35.57812669091055	37.0	37.0	37.0	37.0	37.0
76-77	35.55141117883027	37.0	37.0	37.0	37.0	37.0
78-79	35.503611329370315	37.0	37.0	37.0	37.0	37.0
80-81	35.499489467667665	37.0	37.0	37.0	37.0	37.0
82-83	35.46220575805429	37.0	37.0	37.0	37.0	37.0
84-85	35.417029925867496	37.0	37.0	37.0	37.0	37.0
86-87	35.377522677548484	37.0	37.0	37.0	37.0	37.0
88-89	35.405492160429304	37.0	37.0	37.0	37.0	37.0
90-91	35.34259707993459	37.0	37.0	37.0	37.0	37.0
92-93	35.38480282768465	37.0	37.0	37.0	37.0	37.0
94-95	35.33147406567874	37.0	37.0	37.0	37.0	37.0
96-97	35.304218876810026	37.0	37.0	37.0	37.0	37.0
98-99	35.298512356786716	37.0	37.0	37.0	37.0	37.0
100-101	35.27168658274513	37.0	37.0	37.0	31.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	4645.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	29.0
12	948.0
13	6893.0
14	14644.0
15	18037.0
16	21031.0
17	26650.0
18	31154.0
19	35199.0
20	42237.0
21	60370.0
22	89277.0
23	130365.0
24	171266.0
25	215653.0
26	261143.0
27	335899.0
28	432783.0
29	580494.0
30	774974.0
31	1112986.0
32	1633500.0
33	2735295.0
34	5156795.0
35	1.352101E7
36	4.9577236E7
37	8606906.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.069652980644957	17.375987420711436	11.438821819890833	46.11553777875277
2	22.61776898236213	19.709064202588586	31.683253421023565	25.98991339402572
3	23.551174551150098	19.91157529494267	23.353590994911762	33.18365915899547
4	24.56239740896234	24.712643449866594	21.247995379769797	29.476963761401265
5	26.050742725200397	28.578871164433984	23.48620212739024	21.884183982975383
6	21.913060324710482	33.123500603313346	23.409450579963487	21.55398849201268
7	19.08503832070386	22.883325477334445	37.625063286599044	20.40657291536265
8	19.493557773952638	24.70021140119598	30.32136643336239	25.484864391488987
9	20.322877263514727	22.87514768186267	32.3465186990322	24.4554563555904
10-11	22.265025889723937	30.970125405549126	24.632784436919	22.132064267807934
12-13	21.795213402087672	26.558236889081538	27.701355258989825	23.94519444984096
14-15	21.914939161735887	27.042806225166636	27.31750293695982	23.72475167613766
16-17	22.617588948882442	26.97789021161336	26.63410776939113	23.77041307011307
18-19	22.469600848618835	27.253089081114858	26.39946573030361	23.877844339962696
20-21	22.644838549977006	27.14283295021374	26.611286609864994	23.60104188994426
22-23	22.4715228201502	27.18968870050233	26.657481513224035	23.68130696612344
24-25	22.2574401135801	27.197842404317115	26.768819185731264	23.77589829637153
26-27	22.397602249603796	27.267730139610414	26.749634721656257	23.585032889129536
28-29	22.35757220802495	27.221731458774823	26.614849243723583	23.805847089476643
30-31	22.29141113616376	27.082325100357117	26.730978947003024	23.8952848164761
32-33	22.546381855277716	27.1541316615365	26.602803362548865	23.696683120636916
34-35	22.57313325447393	27.121946320769574	26.420197151620833	23.88472327313566
36-37	22.40909300772148	27.0157053338366	26.59974124657803	23.97546041186389
38-39	22.53622660324738	27.19003575955124	26.591679887781794	23.682057749419588
40-41	22.693626581944585	27.043804837985142	26.46236467359804	23.800203906472234
42-43	22.546790359848465	26.949657797479365	26.713634804971665	23.789917037700505
44-45	22.62070337809475	26.94772811094712	26.741123668737192	23.690444842220934
46-47	22.647053994840686	26.968496840850165	26.588306305054633	23.79614285925452
48-49	22.481727608117964	26.896171158384462	26.675057406767603	23.947043826729967
50-51	22.673616411404783	26.98940127974867	26.55407798023619	23.782904328610353
52-53	22.928347021375565	27.046183069270178	26.339010450302375	23.686459459051882
54-55	22.67047589888376	26.94504971205804	26.558339799562482	23.82613458949573
56-57	22.648549858161672	27.112445919550186	26.571168712679384	23.667835509608757
58-59	22.796182518374202	26.97044830716113	26.490134920113807	23.74323425435086
60-61	22.760623163272527	26.82811168610791	26.52957460179059	23.88169054882897
62-63	22.847196293480458	26.816518570069487	26.623230750814585	23.713054385635466
64-65	22.923873050550977	26.90733037759297	26.42019398173962	23.74860259011643
66-67	22.839594924466542	26.89133846366393	26.52564606915725	23.743420542712276
68-69	22.898329789560236	26.982747827275695	26.495983175777678	23.622939207386395
70-71	23.07763252561047	26.9398447457256	26.32170103894532	23.660821689718606
72-73	22.825479894696805	26.998727426638546	26.40941853905726	23.76637413960739
74-75	22.88663230970573	27.017082660773383	26.437527901424822	23.65875712809606
76-77	22.997872322691816	26.984916158857608	26.289410545910645	23.727800972539928
78-79	22.795225372839102	26.89691027405363	26.502459290828295	23.805405062278965
80-81	22.949657742090057	26.949917864239325	26.41832464091818	23.682099752752436
82-83	23.016457249836304	26.95690773603631	26.28607542038704	23.740559593740347
84-85	22.970163448029083	26.86562421025339	26.3841709719077	23.780041369809826
86-87	23.02828163352464	26.895620740658146	26.395593975528232	23.680503650288987
88-89	23.181743143401555	26.787306506227242	26.268219043853986	23.762731306517217
90-91	22.994205109756827	26.759246770049028	26.454548243786164	23.791999876407985
92-93	23.120618102012102	26.857597346963587	26.39598102638595	23.625803524638357
94-95	23.19572468455789	26.77095762077798	26.311433789681153	23.721883904982974
96-97	23.020279060097348	26.642881038174544	26.42793996444219	23.90889993728592
98-99	23.209652649917615	25.863879432721532	26.903737297368863	24.022730619991993
100-101	24.00912479910237	12.280702467253594	33.322606853739636	30.38756587990441
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	5116.0
1	2717.5
2	358.0
3	527.5
4	796.0
5	1062.0
6	1270.5
7	1463.0
8	1679.0
9	1858.0
10	2058.0
11	2313.0
12	2617.5
13	2926.5
14	3297.5
15	4003.0
16	5022.0
17	6180.0
18	7592.5
19	9497.5
20	11942.0
21	15326.0
22	19798.5
23	25816.5
24	34927.5
25	46751.0
26	60267.5
27	81512.0
28	113885.5
29	154013.5
30	207678.5
31	281245.5
32	383821.0
33	514699.0
34	687677.5
35	921631.0
36	1218864.5
37	1573682.0
38	2019826.0
39	2538110.5
40	3059119.0
41	3574625.5
42	4101801.0
43	4550977.5
44	4903357.5
45	5136053.0
46	5219461.5
47	5197454.5
48	4996100.0
49	4624825.5
50	4202026.5
51	3785042.0
52	3364147.5
53	2930104.0
54	2481975.5
55	2111321.5
56	1792743.5
57	1473669.5
58	1196772.5
59	985502.0
60	826659.5
61	694998.5
62	594115.0
63	510478.0
64	449289.0
65	398088.5
66	349317.5
67	298799.5
68	231215.5
69	172264.5
70	135813.0
71	102081.0
72	75142.0
73	58966.0
74	47581.0
75	34585.5
76	20725.5
77	13949.5
78	10619.0
79	9329.0
80	7732.0
81	5915.5
82	4487.0
83	3387.0
84	3114.0
85	2820.5
86	2523.5
87	2200.0
88	1873.5
89	1644.0
90	1439.0
91	1265.0
92	1135.0
93	1050.0
94	983.5
95	919.5
96	877.5
97	913.0
98	998.5
99	1229.5
100	6679.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05225274374219157
2	0.021014652322636034
3	0.006163737250068252
4	0.006013031771436941
5	0.0059931713595242865
6	0.006904413788457803
7	0.005437079825969986
8	0.006060930411932164
9	0.0054289020093000705
10-11	0.005462197405741872
12-13	0.005449346550974861
14-15	0.005441752864067081
16-17	0.00625194084415092
18-19	0.005482057817654526
20-21	0.005483226077178799
22-23	0.007049862099229884
24-25	0.006617606075248601
26-27	0.007622893395886154
28-29	0.00866498089153833
30-31	0.009722255761006065
32-33	0.011292980691392108
34-35	0.012265556745349996
36-37	1.9746154767895724E-4
38-39	5.439027522910588E-4
40-41	6.543285616228237E-4
42-43	0.0011158802560932335
44-45	8.991459270488965E-4
46-47	6.158000840432738E-4
48-49	6.047101557617677E-4
50-51	0.00102656582327497
52-53	3.797845498608589E-4
54-55	3.0383477109894676E-5
56-57	8.35566032969297E-5
58-59	1.8698396115272645E-5
60-61	4.557842767408533E-5
62-63	5.843553635597573E-6
64-65	1.811554323719393E-5
66-67	7.597078093638313E-6
68-69	1.577901754752399E-5
70-71	1.636393637622055E-5
72-73	0.0
74-75	7.013620128832603E-6
76-77	3.6822945057413024E-5
78-79	4.617670906532209E-5
80-81	2.636287192006104E-4
82-83	0.0015532100596242094
84-85	1.5725850971102867E-4
86-87	0.0012698366966622519
88-89	5.244555861600369E-4
90-91	0.002234851099537093
92-93	0.0010035952102354173
94-95	0.001078611404335027
96-97	0.0018269997109124302
98-99	0.00168596106421964
100-101	3.5667016436693045E-4
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
34-35	10913.0
36-37	1065.0
38-39	1203.0
40-41	1228.0
42-43	1418.0
44-45	1496.0
46-47	1508.0
48-49	1566.0
50-51	1683.0
52-53	2023.0
54-55	2109.0
56-57	1901.0
58-59	1921.0
60-61	2363.0
62-63	2483.0
64-65	2665.0
66-67	2572.0
68-69	2682.0
70-71	2770.0
72-73	3210.0
74-75	3322.0
76-77	3410.0
78-79	3904.0
80-81	4383.0
82-83	4436.0
84-85	5137.0
86-87	5169.0
88-89	6819.0
90-91	16088.0
92-93	11242.0
94-95	33045.0
96-97	620917.0
98-99	7839533.0
100-101	7.6991235E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	14.225539669393633
#Duplication Level	Percentage of deduplicated	Percentage of total
1	50.93214993730935	7.245373193806986
2	5.210421723025383	1.4824212183033583
3	4.36704445324687	1.863706923230064
4	4.391556285285455	2.498890325868128
5	4.007701607110059	2.850585909751838
6	3.8039441733366313	3.2467895242775415
7	3.2816935556427786	3.267870330101285
8	2.839776105382514	3.2317878111452085
9	2.5267989778647713	3.235057118657873
>10	17.307415018896194	44.408820021773245
>50	0.861808982604664	8.372823358351328
>100	0.42437643886075055	11.21612978011142
>500	0.03052972785570525	2.94157792409924
>1k	0.014176998394502973	3.454409857493072
>5k	5.018563247227638E-4	0.4567497308624648
>10k+	1.0415885984812079E-4	0.22700697216707336
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.1682595242737401E-6	0.0	0.0	0.0	3.50477857282122E-6
2	1.1682595242737401E-6	1.1682595242737401E-6	0.0	1.051433571846366E-5	3.50477857282122E-6
3	1.1682595242737401E-6	1.1682595242737401E-6	0.0	1.051433571846366E-5	5.841297621368701E-6
4	1.1682595242737401E-6	1.1682595242737401E-6	1.1682595242737401E-6	1.1682595242737402E-5	1.635563333983236E-5
5	1.1682595242737401E-6	1.1682595242737401E-6	1.1682595242737401E-6	1.1682595242737402E-5	1.635563333983236E-5
6	4.6730380970949604E-6	1.1682595242737401E-6	5.841297621368701E-6	1.1682595242737402E-5	1.635563333983236E-5
7	4.6730380970949604E-6	1.1682595242737401E-6	1.1682595242737402E-5	1.1682595242737402E-5	1.635563333983236E-5
8	9.346076194189921E-6	1.1682595242737401E-6	1.1682595242737402E-5	1.1682595242737402E-5	1.635563333983236E-5
9	9.346076194189921E-6	1.1682595242737401E-6	2.803822858256976E-5	1.8692152388379842E-5	1.8692152388379842E-5
10-11	1.1098465480600531E-5	1.75238928641061E-6	3.387952620393846E-5	1.9860411912653583E-5	2.9206488106843502E-5
12-13	1.9860411912653583E-5	2.3365190485474802E-6	3.8552564301033425E-5	2.161280119906419E-5	2.9206488106843502E-5
14-15	3.972082382530716E-5	2.3365190485474802E-6	5.023515954377082E-5	2.2196930961201062E-5	2.9206488106843502E-5
16-17	4.8482770257360215E-5	2.3365190485474802E-6	6.191775478650822E-5	2.2196930961201062E-5	2.9206488106843502E-5
18-19	5.490819764086578E-5	1.051433571846366E-5	6.892731193215067E-5	2.2196930961201062E-5	2.9790617868980373E-5
20-21	5.8412976213687004E-5	1.8692152388379842E-5	7.59368690777931E-5	3.154300715539098E-5	3.0374747631117243E-5
22-23	7.126383098069814E-5	1.986041191265358E-5	8.995598336907798E-5	3.4463655966075334E-5	3.0374747631117243E-5
24-25	9.871792980113104E-5	2.394932024761167E-5	8.995598336907798E-5	3.7384304776759684E-5	3.0374747631117243E-5
26-27	1.267561583837008E-4	2.6869969058296023E-5	9.404489170403607E-5	3.9720823825307166E-5	3.0374747631117243E-5
28-29	1.775754476896085E-4	2.6869969058296023E-5	1.1098465480600531E-4	3.9720823825307166E-5	3.621604525248594E-5
30-31	2.2547408818483184E-4	2.803822858256976E-5	1.250037690972902E-4	4.030495358744403E-5	4.614625120881273E-5
32-33	2.7220446915578146E-4	2.803822858256976E-5	1.250037690972902E-4	4.965102978163395E-5	5.4324067878728914E-5
34-35	3.2711266679664726E-4	2.803822858256976E-5	1.3434984529148012E-4	5.8412976213687004E-5	7.00955714564244E-5
36-37	3.6566523109768064E-4	2.803822858256976E-5	1.4603244053421752E-4	5.8412976213687004E-5	7.243209050497188E-5
38-39	4.229099477870939E-4	2.803822858256976E-5	1.5654677625268117E-4	5.8412976213687004E-5	7.768925836420371E-5
40-41	4.7489749661727536E-4	2.803822858256976E-5	1.6822937149541857E-4	5.8412976213687004E-5	8.353055598557242E-5
42-43	5.543391442678897E-4	3.8552564301033425E-5	2.0386128698576762E-4	5.8412976213687004E-5	8.76194643205305E-5
44-45	6.42542738350557E-4	3.91366940631703E-5	2.0795019532072573E-4	5.8412976213687004E-5	8.76194643205305E-5
46-47	7.307463324332244E-4	3.9720823825307166E-5	2.143756227042313E-4	5.8412976213687004E-5	8.76194643205305E-5
48-49	8.107721098459756E-4	3.9720823825307166E-5	2.535123167674016E-4	5.8412976213687004E-5	8.76194643205305E-5
50-51	8.861248491616318E-4	5.198754883018143E-5	2.552647060538122E-4	5.8412976213687004E-5	9.638141075258356E-5
52-53	9.819221301520784E-4	6.42542738350557E-5	2.6811556082082335E-4	6.308601431078196E-5	1.1156878456814218E-4
54-55	0.0010917385254338103	6.542253335932944E-5	2.751251179664658E-4	6.775905240787692E-5	1.2325137981087958E-4
56-57	0.0012073962183369103	7.00955714564244E-5	2.821346751121082E-4	6.775905240787692E-5	1.4252766196139627E-4
58-59	0.0013043617588516308	9.229250241762546E-5	2.838870643985188E-4	6.775905240787692E-5	1.6121981434977613E-4
60-61	0.0014264448791382366	9.229250241762546E-5	3.002426977383512E-4	7.710512860206685E-5	1.6121981434977613E-4
62-63	0.001528667587512189	9.229250241762546E-5	3.4054765132579524E-4	7.710512860206685E-5	1.6121981434977613E-4
64-65	0.001658928524468711	9.462902146617295E-5	3.6741762038409125E-4	7.710512860206685E-5	1.6238807387404985E-4
66-67	0.0018055450947650651	9.696554051472043E-5	3.6917000967050186E-4	7.710512860206685E-5	1.6647698220900796E-4
68-69	0.0019305488638623554	9.696554051472043E-5	3.6917000967050186E-4	7.710512860206685E-5	1.711500203061029E-4
70-71	0.0020789178234451207	9.696554051472043E-5	3.6917000967050186E-4	7.710512860206685E-5	1.758230584031979E-4
72-73	0.002203337462780274	9.696554051472043E-5	3.82020864437513E-4	7.710512860206685E-5	1.810802262624297E-4
74-75	0.0023628048878436394	9.696554051472043E-5	3.855256430103342E-4	7.710512860206685E-5	1.8400087507311404E-4
76-77	0.00248956104622734	9.813380003899418E-5	3.855256430103342E-4	7.710512860206685E-5	1.9042630245661964E-4
78-79	0.002612228296276083	9.930205956326791E-5	3.855256430103342E-4	7.827338812634058E-5	1.9159456198089336E-4
80-81	0.002729054248703457	9.930205956326791E-5	3.9487171920452415E-4	7.827338812634058E-5	1.9159456198089336E-4
82-83	0.0028125848046890293	1.0572748694677347E-4	3.9487171920452415E-4	7.944164765061433E-5	1.9159456198089336E-4
84-85	0.0029323314059270878	1.1215291433027904E-4	3.9487171920452415E-4	7.944164765061433E-5	1.9159456198089336E-4
86-87	0.0029884078630922272	1.1215291433027904E-4	4.018812763501666E-4	7.944164765061433E-5	1.9159456198089336E-4
88-89	0.0029965856797621432	1.1215291433027904E-4	4.018812763501666E-4	7.944164765061433E-5	1.9159456198089336E-4
90	0.0029965856797621432	1.1215291433027904E-4	4.030495358744403E-4	7.944164765061433E-5	1.9159456198089336E-4
>>END_MODULE
