##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	SRR21290296_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	75361968
Sequences flagged as poor quality	0
Sequence length	35-101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.044716122593826	37.0	37.0	37.0	37.0	37.0
2	36.171917139955795	37.0	37.0	37.0	37.0	37.0
3	36.29673144947595	37.0	37.0	37.0	37.0	37.0
4	36.330095864800136	37.0	37.0	37.0	37.0	37.0
5	36.34753414348203	37.0	37.0	37.0	37.0	37.0
6	36.31269621302883	37.0	37.0	37.0	37.0	37.0
7	36.27774593147568	37.0	37.0	37.0	37.0	37.0
8	36.336181586977666	37.0	37.0	37.0	37.0	37.0
9	36.31894244056896	37.0	37.0	37.0	37.0	37.0
10-11	36.34099063071177	37.0	37.0	37.0	37.0	37.0
12-13	36.320645016860496	37.0	37.0	37.0	37.0	37.0
14-15	36.3178302350066	37.0	37.0	37.0	37.0	37.0
16-17	36.31734891795819	37.0	37.0	37.0	37.0	37.0
18-19	36.312542388755034	37.0	37.0	37.0	37.0	37.0
20-21	36.30576525682026	37.0	37.0	37.0	37.0	37.0
22-23	36.29298974782612	37.0	37.0	37.0	37.0	37.0
24-25	36.262244047289215	37.0	37.0	37.0	37.0	37.0
26-27	36.21629959955398	37.0	37.0	37.0	37.0	37.0
28-29	36.20328484521529	37.0	37.0	37.0	37.0	37.0
30-31	36.19392623212812	37.0	37.0	37.0	37.0	37.0
32-33	36.18896433277857	37.0	37.0	37.0	37.0	37.0
34-35	36.17467763049925	37.0	37.0	37.0	37.0	37.0
36-37	36.17970208256054	37.0	37.0	37.0	37.0	37.0
38-39	36.17214989797065	37.0	37.0	37.0	37.0	37.0
40-41	36.17340442240173	37.0	37.0	37.0	37.0	37.0
42-43	36.15725072647939	37.0	37.0	37.0	37.0	37.0
44-45	36.1560205695692	37.0	37.0	37.0	37.0	37.0
46-47	36.150700433840655	37.0	37.0	37.0	37.0	37.0
48-49	36.12375684827673	37.0	37.0	37.0	37.0	37.0
50-51	36.11742802238132	37.0	37.0	37.0	37.0	37.0
52-53	36.11931139634346	37.0	37.0	37.0	37.0	37.0
54-55	36.1094333895649	37.0	37.0	37.0	37.0	37.0
56-57	36.09367081481214	37.0	37.0	37.0	37.0	37.0
58-59	36.08149234749165	37.0	37.0	37.0	37.0	37.0
60-61	36.0780968026852	37.0	37.0	37.0	37.0	37.0
62-63	36.069783997263116	37.0	37.0	37.0	37.0	37.0
64-65	36.055695314736504	37.0	37.0	37.0	37.0	37.0
66-67	36.051312281771146	37.0	37.0	37.0	37.0	37.0
68-69	36.033208738709575	37.0	37.0	37.0	37.0	37.0
70-71	36.01267628113733	37.0	37.0	37.0	37.0	37.0
72-73	35.99139023879535	37.0	37.0	37.0	37.0	37.0
74-75	35.98783461245566	37.0	37.0	37.0	37.0	37.0
76-77	35.975472106326706	37.0	37.0	37.0	37.0	37.0
78-79	35.960251572344035	37.0	37.0	37.0	37.0	37.0
80-81	35.93128123744097	37.0	37.0	37.0	37.0	37.0
82-83	35.94551738784388	37.0	37.0	37.0	37.0	37.0
84-85	35.93013476603062	37.0	37.0	37.0	37.0	37.0
86-87	35.94431417958859	37.0	37.0	37.0	37.0	37.0
88-89	35.91864103500533	37.0	37.0	37.0	37.0	37.0
90-91	35.89873973703455	37.0	37.0	37.0	37.0	37.0
92-93	35.8874053391478	37.0	37.0	37.0	37.0	37.0
94-95	35.86135384825968	37.0	37.0	37.0	37.0	37.0
96-97	35.86294816329472	37.0	37.0	37.0	37.0	37.0
98-99	35.840886229296075	37.0	37.0	37.0	37.0	37.0
100-101	35.813300779741326	37.0	37.0	37.0	37.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	37012.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	1.0
14	8.0
15	48.0
16	303.0
17	887.0
18	1508.0
19	2413.0
20	3620.0
21	6533.0
22	12903.0
23	26997.0
24	52344.0
25	92794.0
26	146607.0
27	229866.0
28	343795.0
29	505004.0
30	688136.0
31	938299.0
32	1240930.0
33	1780390.0
34	2820799.0
35	6601192.0
36	4.4893957E7
37	1.4935622E7
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.00058932908108	16.03566488120208	11.853227934333905	47.11051785538293
2	21.890262088902126	19.66368463550706	32.68219336040315	25.763859915187666
3	23.315277547696407	20.220159701885706	23.384429850758316	33.08013289965957
4	24.679674555667848	25.487587407286373	20.67306086444976	29.159677172596027
5	25.876711483661257	29.380296877303145	22.9652421948045	21.777749444231095
6	21.378710131606315	33.69957760081533	23.110750970767242	21.81096129681111
7	18.82620548759431	21.92848808301992	38.095172601229265	21.150133828156502
8	19.8300202126902	24.074577620729045	29.46129301423024	26.634109152350515
9	20.76440088537992	22.27146722907968	31.547041676176573	25.417090209363824
10-11	22.735536015447526	30.602956475673217	23.75232784736044	22.90917966151882
12-13	22.03779902089667	25.76539506194499	27.309758711326165	24.88704720583218
14-15	22.120277229511988	26.351906218079524	26.838217640197797	24.689598912210695
16-17	22.87261422506904	26.369342779110276	26.230840859912163	24.527202135908517
18-19	22.71335943721649	26.704112364255135	25.89383549511447	24.68869270341391
20-21	22.83437619633781	26.531917609933934	26.172947481179555	24.460758712548696
22-23	22.71808892398867	26.628199802005486	26.2128976184833	24.440813655522547
24-25	22.59546971896256	26.536399373839796	26.32571417726506	24.54241672993259
26-27	22.70545638248067	26.698790101947555	26.284800936810903	24.310952578760872
28-29	22.586919825935166	26.584320871942918	26.23431792972083	24.594441372401086
30-31	22.63363099462196	26.34282654050852	26.31995273689956	24.703589727969963
32-33	22.821620745572645	26.517758624995793	26.203013233750045	24.457607395681517
34-35	22.82448753354172	26.52544706569051	26.042587850800075	24.607477549967697
36-37	22.61755700491951	26.424344705956727	26.242618804380392	24.715479484743373
38-39	22.898614275623057	26.574876827974414	26.23552692100019	24.29098197540234
40-41	23.028329614650257	26.444883673775905	25.97440820214864	24.552378509425196
42-43	22.8457063319987	26.36527897532495	26.244492121246743	24.544522571429603
44-45	22.964035915673527	26.379488487289887	26.29490271736766	24.361572879668927
46-47	22.94719289409693	26.31307687586682	26.168252739187093	24.57147749084916
48-49	22.781297288393084	26.17544670628229	26.284304714987368	24.758951290337258
50-51	22.99828500849129	26.24811994507395	26.186704242001003	24.566890804433754
52-53	23.21199509473285	26.391695243926144	25.898609316587784	24.497700344753227
54-55	22.848098910946558	26.31289020622205	26.180557173730328	24.658453709101064
56-57	22.926624565648723	26.437306623044392	26.198419314573236	24.43764949673365
58-59	22.995555712634665	26.32418869264559	26.124995954869778	24.55525963984996
60-61	22.982390758535004	26.189895113559363	26.132313804923	24.69540032298263
62-63	23.09640703681333	26.123621147136806	26.216412515037174	24.563559301012695
64-65	23.128364745148332	26.307936338169036	25.9590323964461	24.604666520236528
66-67	23.138732666817464	26.24453580105867	26.014819558208046	24.601911973915822
68-69	23.150878672359497	26.355640402536533	26.104604345713412	24.388876579390555
70-71	23.41621361067463	26.295958792757368	25.9253529403849	24.362474656183103
72-73	23.181548807933304	26.21510072922729	26.004795446915914	24.59855501592349
74-75	23.204150637423048	26.300017648433784	26.053406542615626	24.442425171527546
76-77	23.302227131974558	26.244124554746286	25.95389395183388	24.499754361445277
78-79	23.079170503964423	26.25532938338701	25.992555130289535	24.67294498235903
80-81	23.271866368384952	26.33105338912445	25.955074481597297	24.442005760893306
82-83	23.35822089577845	26.340507737312578	25.78351190284951	24.51775946405946
84-85	23.247496402551526	26.32232590859442	25.88070484291627	24.549472845937785
86-87	23.27148720072815	26.309007152406274	25.893060134788065	24.526445512077512
88-89	23.432237359882922	26.12780013725277	25.833722361487116	24.606240141377185
90-91	23.285940072122205	26.131310644712098	26.056366143393916	24.52638313977178
92-93	23.460636723405738	26.148166668601842	26.0410633166653	24.35013329132712
94-95	23.44107697103157	26.150702078535232	25.962265104622517	24.445955845810676
96-97	23.315660511891107	26.024575065154576	26.040090086948542	24.61967433600578
98-99	23.498368340731787	25.24299108326006	26.47309573507005	24.785544840938105
100-101	24.30814916140185	11.843465148080835	32.542311521497005	31.306074169020313
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	37091.0
1	18573.0
2	49.5
3	47.0
4	63.5
5	87.0
6	96.5
7	96.5
8	106.0
9	127.5
10	170.5
11	252.0
12	354.0
13	500.5
14	745.5
15	1077.5
16	1622.0
17	2399.0
18	3316.0
19	4569.0
20	6053.5
21	8165.5
22	11397.5
23	15683.0
24	21373.5
25	29080.5
26	39017.5
27	54004.5
28	75045.5
29	100788.0
30	138788.5
31	188978.0
32	263020.5
33	365701.0
34	491656.0
35	653742.5
36	874550.0
37	1154841.0
38	1501833.5
39	1932022.0
40	2363173.5
41	2790020.0
42	3263746.5
43	3699101.5
44	4048893.5
45	4310881.0
46	4450965.5
47	4475804.5
48	4353509.0
49	4133958.5
50	3859907.5
51	3554005.5
52	3203720.0
53	2776068.0
54	2377960.5
55	2080031.5
56	1820714.0
57	1548064.5
58	1284024.5
59	1044094.0
60	872561.0
61	768444.0
62	701674.0
63	633091.0
64	552597.0
65	482208.5
66	427588.5
67	371138.5
68	300956.0
69	232905.0
70	179792.0
71	137546.5
72	104065.5
73	81686.5
74	68918.0
75	51935.5
76	33669.0
77	29026.5
78	21374.5
79	13733.5
80	12572.0
81	9081.0
82	3710.5
83	529.5
84	351.5
85	278.0
86	207.5
87	159.5
88	134.5
89	112.5
90	92.5
91	87.0
92	77.0
93	69.0
94	72.5
95	94.0
96	203.5
97	587.5
98	1837.5
99	2137.5
100	799.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.08555907138730773
2	0.05921289104339738
3	0.05091958320409042
4	0.04911230555974866
5	0.049156094225140196
6	0.04911230555974866
7	0.04911230555974866
8	0.04911230555974866
9	0.0491176132767658
10-11	0.04911230555974866
12-13	0.04913287296319013
14-15	0.04921182525382034
16-17	0.04912159406452867
18-19	0.04930802762475629
20-21	0.04914614225573303
22-23	0.04997082878727371
24-25	0.053540931945938564
26-27	0.056534484343614806
28-29	0.059372122553912075
30-31	0.061773201039548226
32-33	0.06421674126132162
34-35	0.0665600983243962
36-37	1.248170616743808E-4
38-39	2.589341631141951E-5
40-41	2.191033763989651E-5
42-43	3.6053268963840205E-4
44-45	8.100552288351242E-5
46-47	1.75294599340057E-4
48-49	1.4741028613305993E-4
50-51	6.9722611379531E-5
52-53	1.1819906878656563E-4
54-55	3.7851164175639494E-5
56-57	0.0
58-59	1.3281773576853146E-6
60-61	0.0
62-63	3.984742236023628E-6
64-65	6.641424957041769E-7
66-67	1.2619069555354995E-5
68-69	0.0
70-71	3.3210134582675573E-6
72-73	7.970712837853276E-6
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	5.8463269056069334E-5
84-85	5.315084169008515E-6
86-87	8.637448883494456E-6
88-89	2.6179514017322437E-4
90-91	5.914114550697375E-5
92-93	4.652390527190551E-6
94-95	5.656699920351539E-4
96-97	2.9946631708344137E-5
98-99	2.16678778558974E-5
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
34-35	51389.0
36-37	1468.0
38-39	1794.0
40-41	1690.0
42-43	1709.0
44-45	1725.0
46-47	1742.0
48-49	1636.0
50-51	1710.0
52-53	1736.0
54-55	1891.0
56-57	1824.0
58-59	1976.0
60-61	1952.0
62-63	2124.0
64-65	2153.0
66-67	2276.0
68-69	2314.0
70-71	2635.0
72-73	2550.0
74-75	2814.0
76-77	2852.0
78-79	2991.0
80-81	3290.0
82-83	3275.0
84-85	3780.0
86-87	3864.0
88-89	4911.0
90-91	13061.0
92-93	8984.0
94-95	28192.0
96-97	538699.0
98-99	6764305.0
100-101	6.7892656E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.633427847868607
#Duplication Level	Percentage of deduplicated	Percentage of total
1	59.39383483526234	15.224675798579224
2	16.366300828344603	8.39048782839767
3	7.499735716306138	5.767318030860498
4	4.2317272558992505	4.338947011438098
5	2.636561871284787	3.379205924701001
6	1.789119104766515	2.7516753289965443
7	1.2614546859097777	2.263478537223683
8	0.9945497960335342	2.039497635019043
9	0.7362408914218735	1.6985139992021756
>10	4.415627891636046	21.05172929203346
>50	0.3721375324158549	6.613974441296603
>100	0.2589504357217282	12.967838657804434
>500	0.027170668459883814	4.80944347911004
>1k	0.01596773610066618	7.400441127560119
>5k	5.025122583827721E-4	0.8399045547302598
>10k+	1.182381784430052E-4	0.4628683530469837
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	1.326929254289113E-6	3.980787762867339E-6	0.0	2.653858508578226E-6
2	0.0	6.634646271445565E-6	6.634646271445565E-6	2.653858508578226E-6	3.980787762867339E-6
3	0.0	6.634646271445565E-6	7.961575525734678E-6	2.653858508578226E-6	1.5923151051469357E-5
4	1.326929254289113E-5	6.634646271445565E-6	1.4596221797180244E-5	3.980787762867339E-6	1.9903938814336694E-5
5	2.3884726577204035E-5	6.634646271445565E-6	2.3884726577204035E-5	5.307717017156452E-6	2.3884726577204035E-5
6	3.1846302102938714E-5	6.634646271445565E-6	3.5827089865806055E-5	5.307717017156452E-6	2.5211655831493147E-5
7	3.4500160611516937E-5	6.634646271445565E-6	3.5827089865806055E-5	5.307717017156452E-6	2.7865514340071373E-5
8	3.980787762867339E-5	7.961575525734678E-6	3.5827089865806055E-5	9.288504780023792E-6	3.1846302102938714E-5
9	3.980787762867339E-5	7.961575525734678E-6	3.980787762867339E-5	1.0615434034312905E-5	3.848094837438428E-5
10-11	5.0423311662986293E-5	1.0615434034312905E-5	4.644252390011895E-5	1.0615434034312905E-5	4.047134225581795E-5
12-13	6.435606883302198E-5	1.5923151051469357E-5	6.302913957873287E-5	1.127889866145746E-5	4.8432917781552626E-5
14-15	8.890426003737057E-5	1.7250080305758468E-5	6.767339196874476E-5	1.1942363288602018E-5	5.8384887188720975E-5
16-17	1.0748126959741816E-4	1.924047418719214E-5	8.956772466451514E-5	1.1942363288602018E-5	6.302913957873287E-5
18-19	1.2406788527603206E-4	2.123086806862581E-5	9.819276481739436E-5	1.1942363288602018E-5	6.634646271445565E-5
20-21	1.313659961746222E-4	2.1894332695770365E-5	1.1544284512315283E-4	1.326929254289113E-5	7.099071510446755E-5
22-23	1.3733717781892318E-4	2.5211655831493147E-5	1.340198546832004E-4	1.4596221797180244E-5	7.430803824019033E-5
24-25	1.5790458126040444E-4	2.5211655831493147E-5	1.3866410707321232E-4	1.6586615678613913E-5	7.430803824019033E-5
26-27	1.8510663097333127E-4	2.653858508578226E-5	1.4397182409036876E-4	1.7250080305758468E-5	7.497150286733489E-5
28-29	2.1894332695770365E-4	2.7865514340071373E-5	1.6586615678613914E-4	1.7913544932903024E-5	8.293307839306957E-5
30-31	2.461453766706305E-4	3.05193728486496E-5	1.738277323118738E-4	1.8577009560047583E-5	8.824079541022602E-5
32-33	2.614050630949553E-4	3.1846302102938714E-5	1.831162370918976E-4	1.9903938814336694E-5	9.62023709359607E-5
34-35	2.7998207265500285E-4	3.3173231357227825E-5	2.275683671105829E-4	2.123086806862581E-5	1.0151008795311714E-4
36-37	3.091745162493633E-4	3.3173231357227825E-5	2.4548191204348596E-4	2.255779732291492E-5	1.068178049702736E-4
38-39	3.5827089865806056E-4	3.4500160611516937E-5	2.607415984678107E-4	2.3884726577204035E-5	1.2473134990317663E-4
40-41	3.781748374723972E-4	4.047134225581795E-5	2.793186080278583E-4	2.5211655831493147E-5	1.360102485646341E-4
42-43	3.947614531510111E-4	4.246173613725162E-5	3.025398699779178E-4	2.653858508578226E-5	1.40654500954646E-4
44-45	4.246173613725162E-4	4.378866539154073E-5	3.0585719311364057E-4	2.7202049712926817E-5	1.4463528871751333E-4
46-47	4.584540573568885E-4	4.378866539154073E-5	3.2708806118226635E-4	2.852897896721593E-5	1.4861607648038066E-4
48-49	5.108677629013085E-4	4.378866539154073E-5	3.383669598437238E-4	2.9192443594360488E-5	1.6387576290470546E-4
50-51	5.533294990385601E-4	4.511559464582984E-5	3.443381414880248E-4	2.9192443594360488E-5	1.831162370918976E-4
52-53	5.944643059215225E-4	4.57790592729744E-5	3.668959388109398E-4	2.9855908221505047E-5	1.890874187361986E-4
54-55	6.488684053473763E-4	4.8432917781552626E-5	3.93434523896722E-4	3.118283747579416E-5	1.9572206500764419E-4
56-57	6.919936061117724E-4	5.241370554441997E-5	4.2329043211822705E-4	3.1846302102938714E-5	2.136356099405472E-4
58-59	7.34455342249024E-4	5.4404099425853634E-5	4.3589626003397364E-4	3.1846302102938714E-5	2.2624143785629378E-4
60-61	7.815613307762875E-4	5.5731028680142746E-5	4.869830363241045E-4	3.1846302102938714E-5	2.3022222561916114E-4
62-63	8.253499961678283E-4	5.63944933072873E-5	5.334255602242235E-4	3.4500160611516937E-5	2.3287608412773936E-4
64-65	8.664848030507908E-4	5.971181644301009E-5	5.593006806828612E-4	3.516362523866149E-5	2.4017419502632946E-4
66-67	9.315043365109574E-4	5.971181644301009E-5	5.632814684457285E-4	3.5827089865806055E-5	2.4548191204348596E-4
68-69	9.958604053439794E-4	5.971181644301009E-5	5.712430439714631E-4	3.5827089865806055E-5	2.481357705520641E-4
70-71	0.0010628703326855797	5.971181644301009E-5	5.74560367107186E-4	3.5827089865806055E-5	2.647223862306781E-4
72-73	0.001131207189281469	6.369260420587743E-5	5.911469827857999E-4	3.5827089865806055E-5	2.8595325429930387E-4
74-75	0.0012021979043859363	6.767339196874476E-5	5.977816290572454E-4	3.5827089865806055E-5	2.9656868833361676E-4
76-77	0.0012546116099303563	6.833685659588932E-5	6.024258814472574E-4	3.5827089865806055E-5	3.0983798087650793E-4
78-79	0.0013063618508476317	7.032725047732298E-5	6.037528107015464E-4	3.5827089865806055E-5	3.1647262714795346E-4
80-81	0.001355458233256329	7.165417973161211E-5	6.050797399558355E-4	3.980787762867339E-5	3.250976673008327E-4
82-83	0.0013846506768506895	7.165417973161211E-5	6.070701338372693E-4	4.246173613725162E-5	3.416842829794466E-4
84-85	0.0014145065850721945	7.298110898590122E-5	6.09060527718703E-4	4.4452130018685285E-5	3.522997170137595E-4
86-87	0.0014297662714965191	7.563496749447944E-5	6.117143862272811E-4	4.511559464582984E-5	3.5959782791234965E-4
88-89	0.001433083594632242	7.696189674876855E-5	6.137047801087148E-4	4.57790592729744E-5	3.7618444359096355E-4
90	0.001433083594632242	7.696189674876855E-5	6.183490324987267E-4	4.644252390011896E-5	3.8215562523526457E-4
>>END_MODULE
