##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	SRR21290292_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	83758105
Sequences flagged as poor quality	0
Sequence length	35-101
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.97469114183039	37.0	37.0	37.0	37.0	37.0
2	36.134792555299576	37.0	37.0	37.0	37.0	37.0
3	36.26762849995233	37.0	37.0	37.0	37.0	37.0
4	36.3026507703344	37.0	37.0	37.0	37.0	37.0
5	36.319628291494894	37.0	37.0	37.0	37.0	37.0
6	36.28313213389916	37.0	37.0	37.0	37.0	37.0
7	36.25088715892032	37.0	37.0	37.0	37.0	37.0
8	36.3121368732017	37.0	37.0	37.0	37.0	37.0
9	36.29624697215869	37.0	37.0	37.0	37.0	37.0
10-11	36.31661589048606	37.0	37.0	37.0	37.0	37.0
12-13	36.2969597151225	37.0	37.0	37.0	37.0	37.0
14-15	36.29300978693345	37.0	37.0	37.0	37.0	37.0
16-17	36.29228312292882	37.0	37.0	37.0	37.0	37.0
18-19	36.2881762009778	37.0	37.0	37.0	37.0	37.0
20-21	36.28138772361194	37.0	37.0	37.0	37.0	37.0
22-23	36.26866466236312	37.0	37.0	37.0	37.0	37.0
24-25	36.23565936096573	37.0	37.0	37.0	37.0	37.0
26-27	36.190275448567036	37.0	37.0	37.0	37.0	37.0
28-29	36.176547224892445	37.0	37.0	37.0	37.0	37.0
30-31	36.16618905716647	37.0	37.0	37.0	37.0	37.0
32-33	36.160299710696655	37.0	37.0	37.0	37.0	37.0
34-35	36.14592891637173	37.0	37.0	37.0	37.0	37.0
36-37	36.16726935609327	37.0	37.0	37.0	37.0	37.0
38-39	36.16041535649029	37.0	37.0	37.0	37.0	37.0
40-41	36.16144692801279	37.0	37.0	37.0	37.0	37.0
42-43	36.143286288923555	37.0	37.0	37.0	37.0	37.0
44-45	36.14173823727104	37.0	37.0	37.0	37.0	37.0
46-47	36.13723656340912	37.0	37.0	37.0	37.0	37.0
48-49	36.109648070708126	37.0	37.0	37.0	37.0	37.0
50-51	36.10322414843499	37.0	37.0	37.0	37.0	37.0
52-53	36.10521724419566	37.0	37.0	37.0	37.0	37.0
54-55	36.09391049154863	37.0	37.0	37.0	37.0	37.0
56-57	36.07796098622684	37.0	37.0	37.0	37.0	37.0
58-59	36.06766498352363	37.0	37.0	37.0	37.0	37.0
60-61	36.0638097913895	37.0	37.0	37.0	37.0	37.0
62-63	36.05322552049304	37.0	37.0	37.0	37.0	37.0
64-65	36.03670847057511	37.0	37.0	37.0	37.0	37.0
66-67	36.0353023921311	37.0	37.0	37.0	37.0	37.0
68-69	36.01841666950489	37.0	37.0	37.0	37.0	37.0
70-71	35.9979251498622	37.0	37.0	37.0	37.0	37.0
72-73	35.97423379026823	37.0	37.0	37.0	37.0	37.0
74-75	35.96969444563544	37.0	37.0	37.0	37.0	37.0
76-77	35.95538807758922	37.0	37.0	37.0	37.0	37.0
78-79	35.94232188844525	37.0	37.0	37.0	37.0	37.0
80-81	35.912188469211145	37.0	37.0	37.0	37.0	37.0
82-83	35.92512080081788	37.0	37.0	37.0	37.0	37.0
84-85	35.90786175783482	37.0	37.0	37.0	37.0	37.0
86-87	35.921935787451815	37.0	37.0	37.0	37.0	37.0
88-89	35.89792794176836	37.0	37.0	37.0	37.0	37.0
90-91	35.87940286340477	37.0	37.0	37.0	37.0	37.0
92-93	35.86828669126338	37.0	37.0	37.0	37.0	37.0
94-95	35.83945456242813	37.0	37.0	37.0	37.0	37.0
96-97	35.84026162227843	37.0	37.0	37.0	37.0	37.0
98-99	35.819074008845	37.0	37.0	37.0	37.0	37.0
100-101	35.79566334546743	37.0	37.0	37.0	37.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	80088.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	7.0
15	98.0
16	426.0
17	1078.0
18	1929.0
19	2938.0
20	4423.0
21	7598.0
22	14362.0
23	29011.0
24	55505.0
25	98690.0
26	158894.0
27	255476.0
28	383015.0
29	563343.0
30	769877.0
31	1053092.0
32	1400074.0
33	2031039.0
34	3246742.0
35	7650095.0
36	4.9961176E7
37	1.5989129E7
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.983233861858306	15.694036586703344	11.710910486240602	47.61181906519775
2	21.28614361112201	19.284101082061856	33.3743999223806	26.05535538443554
3	23.183034249888166	19.995439342756733	23.319037728808468	33.50248867854664
4	24.833070554241267	25.24471630344682	20.754743745899237	29.16746939641268
5	25.847978743082024	29.223939697780953	23.15939813175705	21.768683427379976
6	21.450060175302674	33.46222461270802	23.265213132380993	21.822502079608316
7	18.824071798928983	21.666017730797805	38.25217798839569	21.25773248187753
8	19.694737746952107	23.833995731519305	29.67180496163048	26.799461559898102
9	20.74089662043497	22.01995895767941	31.722439120420358	25.516705301465258
10-11	22.718900592493725	30.362347735845603	23.925761170941705	22.992990500718964
12-13	21.92776215452775	25.615642148400454	27.52988856305518	24.92670713401662
14-15	21.985747628605722	26.229922656611464	26.960037475590415	24.8242922391924
16-17	22.94058392964044	26.138433658606562	26.32966968460214	24.591312727150854
18-19	22.76306809467731	26.51814345933069	25.86383055507237	24.854957890919636
20-21	22.80545020510382	26.25308088040381	26.230661005910772	24.710807908581597
22-23	22.69403731564657	26.340406699661884	26.3860900041347	24.57946598055685
24-25	22.61858130329392	26.195949408996334	26.48518002124402	24.700289266465727
26-27	22.698346025872702	26.44304611383606	26.362751460478634	24.4958563998126
28-29	22.550674326038795	26.317805697620244	26.35473649503444	24.776783481306516
30-31	22.653295769805048	26.045173704326213	26.413419923289556	24.888110602579186
32-33	22.861892463105924	26.213635900313324	26.31288721375155	24.611584422829193
34-35	22.89069263088074	26.198932523281194	26.174355781797814	24.736019064040253
36-37	22.721393856524784	26.11692267493229	26.358029153466873	24.803654315076052
38-39	23.003804006189085	26.251832933939717	26.403678092519183	24.34068496735201
40-41	23.108114490354225	26.117267985273994	26.04231660559161	24.73230091878017
42-43	22.89067845685526	26.17426912289141	26.275644251012658	24.659408169240677
44-45	23.03179891924949	26.140681780611715	26.392895990334512	24.434623309804287
46-47	22.964289848829797	26.053324033062665	26.274031922205182	24.708354195902356
48-49	22.805455914309206	25.857868128747146	26.3967673946156	24.939908562328046
50-51	23.03922642238873	25.92985274304945	26.285538542891736	24.74538229167008
52-53	23.318641869857345	26.175750210215288	25.936676215180427	24.568931704746937
54-55	22.872272898539695	26.104699388770747	26.25811808231792	24.76490963037164
56-57	22.942719769298503	26.208285335282945	26.25084184644888	24.598153048969667
58-59	22.981349312465476	26.125238284636133	26.17388877441465	24.719523628483742
60-61	23.02441176860055	25.92132072958004	26.160956803422348	24.893310698397066
62-63	23.118682429922092	25.891167545147514	26.217187387838877	24.77296263709152
64-65	23.15045552797461	26.130150347685728	25.890525088197418	24.828869036142244
66-67	23.267975491194832	26.016289133025968	25.955295851554062	24.76043952422514
68-69	23.255716245803853	26.096197713827536	26.128887796223342	24.519198244145272
70-71	23.606924568850946	26.036098767257737	25.87195432208324	24.485022341808076
72-73	23.359232523607385	25.93027705165228	25.94506763697116	24.765422787769175
74-75	23.341855761071738	26.08677427429597	25.97121754015033	24.60015242448196
76-77	23.416253533310574	26.05800279361003	25.95662594141511	24.56911773166429
78-79	23.180722478295134	26.103775719497513	25.91159580337366	24.803905998833688
80-81	23.39677080070657	26.171384231956946	25.8875483935111	24.54429657382538
82-83	23.47989534680835	26.170128268786286	25.682179697705436	24.667796686699926
84-85	23.34247687046703	26.181795087305403	25.790633489792945	24.685094552434617
86-87	23.34755179642795	26.17114365612407	25.80077348069206	24.68053106675592
88-89	23.545874490667206	25.90255021419931	25.7848973344507	24.766677960682777
90-91	23.42714587706379	25.86096046867807	26.043456933727636	24.6684367205305
92-93	23.657702726084214	25.888605023989914	25.987460965086413	24.46623128483946
94-95	23.563642456860926	25.97489236274697	25.911734068812052	24.54973111158005
96-97	23.49140619800409	25.79669621175175	25.97589028084135	24.73600730940281
98-99	23.692298204857753	25.002724196563108	26.405216514974732	24.899761083604407
100-101	24.52910172918629	11.640741341183865	32.31762756908383	31.51252936054601
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	80205.0
1	40124.5
2	44.5
3	49.0
4	63.0
5	75.5
6	77.5
7	73.5
8	85.0
9	103.5
10	143.0
11	214.5
12	276.5
13	368.0
14	540.0
15	794.0
16	1227.5
17	1828.0
18	2664.5
19	3752.5
20	5069.0
21	6981.5
22	10021.5
23	14583.5
24	20547.5
25	27796.0
26	37126.5
27	53174.5
28	73842.5
29	96869.0
30	133476.0
31	182991.5
32	261445.0
33	372271.0
34	498443.0
35	658966.0
36	890298.5
37	1191823.5
38	1570511.5
39	2063922.5
40	2555606.0
41	3014093.5
42	3546752.0
43	4029188.0
44	4426226.5
45	4739506.0
46	4887925.0
47	4902381.0
48	4789231.5
49	4640012.0
50	4436463.5
51	4162762.5
52	3754763.0
53	3199192.5
54	2721074.5
55	2401883.0
56	2125074.0
57	1812814.5
58	1511159.5
59	1216782.0
60	998448.0
61	879111.5
62	817793.5
63	754310.5
64	640836.5
65	531407.5
66	465691.5
67	413702.5
68	334912.0
69	250619.5
70	181845.0
71	131447.5
72	99781.0
73	77718.0
74	64923.0
75	47190.5
76	28382.5
77	23237.5
78	17043.0
79	11384.0
80	9480.0
81	6008.5
82	2422.5
83	532.0
84	369.5
85	295.0
86	237.5
87	187.5
88	157.5
89	123.5
90	84.5
91	80.5
92	79.0
93	79.0
94	99.5
95	139.5
96	298.5
97	810.5
98	2361.5
99	2755.5
100	1067.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.13173531086931825
2	0.10542740908476857
3	0.09738639621801377
4	0.09561820912734356
5	0.0956599961281359
6	0.09561820912734356
7	0.09561820912734356
8	0.09561820912734356
9	0.09561820912734356
10-11	0.09561820912734356
12-13	0.09564566915643566
14-15	0.09571670705778264
16-17	0.0956271634846562
18-19	0.09581222020245085
20-21	0.0956522356851316
22-23	0.0966199032320514
24-25	0.10054728434937729
26-27	0.10375712296738328
28-29	0.10680041053937406
30-31	0.10944075203229586
32-33	0.11189484289311465
34-35	0.1142235727515564
36-37	1.4941309490429925E-4
38-39	3.4066892950126055E-5
40-41	3.227467382676719E-5
42-43	3.753513442488174E-4
44-45	9.085173531745554E-5
46-47	2.0322583475669123E-4
48-49	1.4823902469194124E-4
50-51	7.173031867700409E-5
52-53	1.1238010365498414E-4
54-55	4.18446506465139E-5
56-57	5.977961610857642E-7
58-59	1.1956227914829426E-6
60-61	5.978272709144431E-7
62-63	8.369814414969073E-6
64-65	2.391445731657381E-6
66-67	5.3809157750663605E-6
68-69	0.0
70-71	1.7937518957715348E-6
72-73	4.185563264014406E-6
74-75	0.0
76-77	1.1959612460478638E-6
78-79	0.0
80-81	1.1960550969239095E-6
82-83	5.262858459494467E-5
84-85	6.578858325619671E-6
86-87	1.196212542278936E-5
88-89	2.781327659042103E-4
90-91	5.622874153538357E-5
92-93	5.384538330491225E-6
94-95	5.887790524092998E-4
96-97	2.5759264350874055E-5
98-99	1.462492069179726E-5
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
34-35	97021.0
36-37	1690.0
38-39	2015.0
40-41	1887.0
42-43	2203.0
44-45	2063.0
46-47	2031.0
48-49	1991.0
50-51	1999.0
52-53	1992.0
54-55	2130.0
56-57	2111.0
58-59	2197.0
60-61	2294.0
62-63	2418.0
64-65	2558.0
66-67	2561.0
68-69	2660.0
70-71	2804.0
72-73	2927.0
74-75	3022.0
76-77	3223.0
78-79	3273.0
80-81	3412.0
82-83	3548.0
84-85	3890.0
86-87	3922.0
88-89	4825.0
90-91	14075.0
92-93	9093.0
94-95	30718.0
96-97	595469.0
98-99	7512550.0
100-101	7.5427533E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.601955718779703
#Duplication Level	Percentage of deduplicated	Percentage of total
1	58.28501759201177	12.590703690909342
2	16.22900638527377	7.011565545889541
3	7.654014343725166	4.960250367721672
4	4.319517160146933	3.732400736800124
5	2.7363234041274205	2.9554968504110537
6	1.880200638722579	2.436960656406388
7	1.3731079496470349	2.076327198776575
8	1.0176079571630323	1.7585857623770957
9	0.812728467662389	1.5800871932851153
>10	4.856254237174354	19.842721731522264
>50	0.44672551378799547	6.692683061459745
>100	0.3235812760563719	13.901273586424265
>500	0.036948591196924535	5.542332326971859
>1k	0.026888108726417396	10.923435289299599
>5k	0.001650103452788278	2.353235833370926
>10k+	4.2827112515115613E-4	1.6419401683741446
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.1939143083526066E-6	1.1939143083526066E-6	0.0	0.0	1.1939143083526066E-6
2	3.58174292505782E-6	3.58174292505782E-6	4.7756572334104265E-6	2.3878286167052132E-6	2.3878286167052132E-6
3	9.551314466820853E-6	3.58174292505782E-6	4.7756572334104265E-6	2.3878286167052132E-6	2.0296543241994313E-5
4	3.1041772017167774E-5	3.58174292505782E-6	1.9102628933641706E-5	2.3878286167052132E-6	2.2684371858699525E-5
5	4.417482940904644E-5	3.58174292505782E-6	2.0296543241994313E-5	2.3878286167052132E-6	2.746002909210995E-5
6	5.014440095080948E-5	4.7756572334104265E-6	2.865394340046256E-5	2.3878286167052132E-6	2.865394340046256E-5
7	5.014440095080948E-5	4.7756572334104265E-6	3.462351494222559E-5	2.3878286167052132E-6	3.58174292505782E-5
8	5.730788680092512E-5	4.7756572334104265E-6	3.462351494222559E-5	2.3878286167052132E-6	3.820525786728341E-5
9	5.730788680092512E-5	5.969571541763033E-6	3.58174292505782E-5	2.3878286167052132E-6	4.2980915100693835E-5
10-11	6.865007273027488E-5	5.969571541763033E-6	4.059308648398863E-5	2.3878286167052132E-6	4.835352948828057E-5
12-13	8.417095873885876E-5	5.969571541763033E-6	4.357787225487014E-5	2.3878286167052132E-6	5.1935272413338384E-5
14-15	1.0088575905579526E-4	5.969571541763033E-6	5.074135810498578E-5	2.9847857708815165E-6	6.088962972598294E-5
16-17	1.1580968791020284E-4	5.969571541763033E-6	6.506832980521707E-5	3.58174292505782E-6	6.805311557609857E-5
18-19	1.259579595312E-4	7.16348585011564E-6	7.16348585011564E-5	3.58174292505782E-6	7.044094419280379E-5
20-21	1.3252448822713932E-4	7.16348585011564E-6	8.894661597226919E-5	4.178700079234123E-6	7.641051573456682E-5
22-23	1.3849405976890236E-4	7.760443004291942E-6	1.0446750198085309E-4	5.37261438758673E-6	7.879834435127204E-5
24-25	1.5401494577748625E-4	8.357400158468245E-6	1.0745228775173459E-4	5.969571541763033E-6	7.879834435127204E-5
26-27	1.772962747903621E-4	9.551314466820853E-6	1.092431592142635E-4	7.16348585011564E-6	7.879834435127204E-5
28-29	1.999806466490616E-4	1.2536100237702368E-5	1.1580968791020284E-4	7.16348585011564E-6	9.312531605150331E-5
30-31	2.2744067574117155E-4	1.432697170023128E-5	1.1879447368108436E-4	7.16348585011564E-6	9.790097328491374E-5
32-33	2.453493903664607E-4	1.671480031693649E-5	1.2774883099372892E-4	7.16348585011564E-6	1.0566141628920568E-4
34-35	2.722124623043943E-4	1.79087146252891E-5	1.5640277439419147E-4	7.16348585011564E-6	1.1222794498514502E-4
36-37	2.996724913965043E-4	1.79087146252891E-5	1.599845173192493E-4	7.16348585011564E-6	1.2058534514361327E-4
38-39	3.2952034910531944E-4	1.79087146252891E-5	1.7013278894024644E-4	7.16348585011564E-6	1.432697170023128E-4
40-41	3.5638342104325304E-4	1.9102628933641706E-5	1.8386280348630141E-4	7.16348585011564E-6	1.6058147447342558E-4
42-43	3.850373644437156E-4	2.328132901287583E-5	2.059502181908246E-4	7.16348585011564E-6	1.623723459359545E-4
44-45	4.166760936150597E-4	2.8056986246286255E-5	2.0654717534500093E-4	1.0745228775173459E-5	1.65357131706836E-4
46-47	4.5846309440740094E-4	2.865394340046256E-5	2.2147110419940854E-4	1.4923928854407583E-5	1.6834191747771752E-4
48-49	5.044287952789763E-4	2.9847857708815166E-5	2.3639503305381612E-4	1.5520886008583887E-5	1.7490844617365687E-4
50-51	5.492005818421991E-4	2.9847857708815166E-5	2.4415547605810805E-4	1.5520886008583887E-5	2.0117456095741424E-4
52-53	5.993449827930086E-4	2.9847857708815166E-5	2.662428907626313E-4	1.6117843162760187E-5	2.029654324199431E-4
54-55	6.33968497735234E-4	2.9847857708815166E-5	2.883303054671545E-4	1.671480031693649E-5	2.0774108965335354E-4
56-57	6.960520417695696E-4	3.1041772017167774E-5	3.122085916342066E-4	1.79087146252891E-5	2.1788936127435069E-4
58-59	7.647021144998445E-4	3.1041772017167774E-5	3.259386061802616E-4	1.9102628933641706E-5	2.3221633297458198E-4
60-61	8.13055643988125E-4	3.1041772017167774E-5	3.611590782766635E-4	1.9102628933641706E-5	2.3639503305381612E-4
62-63	8.60215259168053E-4	3.1041772017167774E-5	3.933947646021839E-4	1.9102628933641706E-5	2.3639503305381612E-4
64-65	9.067779171938047E-4	3.1041772017167774E-5	4.17273050769236E-4	2.0296543241994313E-5	2.45946347520637E-4
66-67	9.640858039947299E-4	3.1041772017167774E-5	4.190639222317649E-4	2.1490457550346917E-5	2.6027331922086827E-4
68-69	0.0010213936907956548	3.1041772017167774E-5	4.2742132239023315E-4	2.2684371858699525E-5	2.7579420522945213E-4
70-71	0.0010775076632882274	3.1041772017167774E-5	4.3219697962364363E-4	2.5072200475404736E-5	2.9549379131727014E-4
72-73	0.0011658573221063203	3.1041772017167774E-5	4.4055437978211186E-4	2.6266114783757344E-5	3.199690346384986E-4
74-75	0.0012458495807659451	3.1041772017167774E-5	4.4174829409046444E-4	2.6266114783757344E-5	3.30714263413672E-4
76-77	0.0012995757246418123	3.163872917134408E-5	4.536874371739905E-4	2.6266114783757344E-5	3.396686207263166E-4
78-79	0.0013431535968966824	3.223568632552038E-5	4.560752657906957E-4	2.9847857708815166E-5	3.456381922680796E-4
80-81	0.0013938949550016681	3.283264347969668E-5	4.572691800990483E-4	3.223568632552038E-5	3.569803781974294E-4
82-83	0.0014344880414856567	3.342960063387298E-5	4.6323875164081137E-4	3.223568632552038E-5	3.5817429250578197E-4
84-85	0.001486423313898995	3.342960063387298E-5	4.668204945658692E-4	3.342960063387298E-5	3.6772560697260283E-4
86-87	0.001499556371290874	3.342960063387298E-5	4.709991946451033E-4	3.342960063387298E-5	3.7369517851436584E-4
88-89	0.001501944199907579	3.4026557788049286E-5	4.7577485187851374E-4	3.342960063387298E-5	3.832464929811867E-4
90	0.001501944199907579	3.7011343558930804E-5	4.7875963764939524E-4	3.342960063387298E-5	3.9279780744800756E-4
>>END_MODULE
