##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	SRR21290300_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	85597419
Sequences flagged as poor quality	0
Sequence length	35-101
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.12077076763261	37.0	37.0	37.0	37.0	37.0
2	36.20588921028098	37.0	37.0	37.0	37.0	37.0
3	36.317049956845075	37.0	37.0	37.0	37.0	37.0
4	36.34523824836354	37.0	37.0	37.0	37.0	37.0
5	36.36115308570227	37.0	37.0	37.0	37.0	37.0
6	36.32922658567544	37.0	37.0	37.0	37.0	37.0
7	36.2919851590385	37.0	37.0	37.0	37.0	37.0
8	36.34317095472236	37.0	37.0	37.0	37.0	37.0
9	36.328260026158034	37.0	37.0	37.0	37.0	37.0
10-11	36.349092009421454	37.0	37.0	37.0	37.0	37.0
12-13	36.33037091924466	37.0	37.0	37.0	37.0	37.0
14-15	36.32493204029902	37.0	37.0	37.0	37.0	37.0
16-17	36.32400630561069	37.0	37.0	37.0	37.0	37.0
18-19	36.3184948602247	37.0	37.0	37.0	37.0	37.0
20-21	36.31318745136463	37.0	37.0	37.0	37.0	37.0
22-23	36.29964477667253	37.0	37.0	37.0	37.0	37.0
24-25	36.27040666378036	37.0	37.0	37.0	37.0	37.0
26-27	36.22535987913374	37.0	37.0	37.0	37.0	37.0
28-29	36.21275864637928	37.0	37.0	37.0	37.0	37.0
30-31	36.202977130653906	37.0	37.0	37.0	37.0	37.0
32-33	36.19677965406878	37.0	37.0	37.0	37.0	37.0
34-35	36.184481450311026	37.0	37.0	37.0	37.0	37.0
36-37	36.191941954265	37.0	37.0	37.0	37.0	37.0
38-39	36.18390054247686	37.0	37.0	37.0	37.0	37.0
40-41	36.18195384331818	37.0	37.0	37.0	37.0	37.0
42-43	36.166671078597886	37.0	37.0	37.0	37.0	37.0
44-45	36.166335083533355	37.0	37.0	37.0	37.0	37.0
46-47	36.1622668974712	37.0	37.0	37.0	37.0	37.0
48-49	36.13424162476015	37.0	37.0	37.0	37.0	37.0
50-51	36.127259328277674	37.0	37.0	37.0	37.0	37.0
52-53	36.12569001831709	37.0	37.0	37.0	37.0	37.0
54-55	36.11621851344147	37.0	37.0	37.0	37.0	37.0
56-57	36.10239137171734	37.0	37.0	37.0	37.0	37.0
58-59	36.09241020303948	37.0	37.0	37.0	37.0	37.0
60-61	36.08926513507874	37.0	37.0	37.0	37.0	37.0
62-63	36.07786018764642	37.0	37.0	37.0	37.0	37.0
64-65	36.06145530354256	37.0	37.0	37.0	37.0	37.0
66-67	36.05708532144602	37.0	37.0	37.0	37.0	37.0
68-69	36.042841827884054	37.0	37.0	37.0	37.0	37.0
70-71	36.01955936661663	37.0	37.0	37.0	37.0	37.0
72-73	35.99603413694757	37.0	37.0	37.0	37.0	37.0
74-75	35.992607235937825	37.0	37.0	37.0	37.0	37.0
76-77	35.97830993740476	37.0	37.0	37.0	37.0	37.0
78-79	35.96637377571887	37.0	37.0	37.0	37.0	37.0
80-81	35.938575402940245	37.0	37.0	37.0	37.0	37.0
82-83	35.95097132098492	37.0	37.0	37.0	37.0	37.0
84-85	35.93248434042925	37.0	37.0	37.0	37.0	37.0
86-87	35.947483347448255	37.0	37.0	37.0	37.0	37.0
88-89	35.922874959325405	37.0	37.0	37.0	37.0	37.0
90-91	35.9059680725847	37.0	37.0	37.0	37.0	37.0
92-93	35.89170721526377	37.0	37.0	37.0	37.0	37.0
94-95	35.86120393627829	37.0	37.0	37.0	37.0	37.0
96-97	35.86008539855265	37.0	37.0	37.0	37.0	37.0
98-99	35.83835469254301	37.0	37.0	37.0	37.0	37.0
100-101	35.8071177077746	37.0	37.0	37.0	37.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	56141.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	10.0
15	104.0
16	610.0
17	1753.0
18	3023.0
19	4380.0
20	5939.0
21	9158.0
22	16365.0
23	32497.0
24	61476.0
25	108091.0
26	169547.0
27	261306.0
28	387465.0
29	565665.0
30	767280.0
31	1041131.0
32	1367706.0
33	1951080.0
34	3100706.0
35	7326712.0
36	5.1322791E7
37	1.7036483E7
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.922896885100116	17.22015291655855	11.2783072197125	46.57864297862883
2	22.085724197082666	19.483327090667764	31.94152230524459	26.489426407004984
3	23.188737653957933	19.741835381772514	23.350684562344235	33.71874240192532
4	24.305461895441706	24.65546135476704	21.21899515760082	29.82008159219044
5	25.813881606827117	28.515508015138895	23.410198003887015	22.260412374146977
6	21.719562104274384	33.08684960259771	23.422093366432986	21.771494926694924
7	18.87737753929746	22.94806607869478	37.561811971058	20.612744410949762
8	19.362891679032433	24.767523347032526	30.24392738205291	25.625657591882135
9	20.141007481101784	22.898872552487145	32.29769880568886	24.662421160722207
10-11	22.076422566424597	30.983417736639378	24.578854199489513	22.36130549744651
12-13	21.64699117127805	26.550220910938183	27.61607497948943	24.186712938294338
14-15	21.75660284482575	27.046812309629008	27.228373364941337	23.9682114806039
16-17	22.45248572845348	26.967241456456005	26.55258606306096	24.027686752029553
18-19	22.327870315086145	27.247174382133956	26.32177609938538	24.10317920339452
20-21	22.488237328051905	27.124048883662947	26.541666750690453	23.846047037594694
22-23	22.351527854962097	27.15265164240518	26.56844525152898	23.927375251103747
24-25	22.13744296209772	27.162583808300546	26.66816467266392	24.03180855693782
26-27	22.2919133700332	27.215173625121025	26.687417709202837	23.805495295642938
28-29	22.23951289618225	27.160090039218026	26.549985090963364	24.05041197363636
30-31	22.185047846417987	27.013454982952755	26.671864745513	24.12963242511626
32-33	22.441235783107196	27.087588195331797	26.542497837008206	23.928678184552805
34-35	22.46333682179391	27.06662295656778	26.33558008712956	24.134460134508746
36-37	22.2760165239004	26.970566429761117	26.525609046864112	24.227807999474372
38-39	22.441947917066177	27.12672147355999	26.53458795662627	23.896742652747562
40-41	22.582995923395753	26.98979112195196	26.411941615192113	24.015271339460178
42-43	22.442104312579442	26.89680298603078	26.645188998364095	24.015903703025685
44-45	22.534801213417783	26.896518882825866	26.651274631220655	23.9174052725357
46-47	22.55413108506762	26.917398116203895	26.491306625561506	24.037164173166975
48-49	22.397559902974727	26.81871113323848	26.595629639695083	24.188099324091702
50-51	22.613217996592876	26.915742607340263	26.441940369431354	24.029099026635503
52-53	22.812316548078854	27.005306051588974	26.26611290143349	23.91626449889868
54-55	22.57930593691844	26.91465371750965	26.45053651858631	24.055503826985596
56-57	22.571582021661172	27.06393517346134	26.49569066303943	23.86879214183806
58-59	22.672446392669876	26.918997329763215	26.42884228260049	23.97971399496642
60-61	22.615077664028355	26.800527882360132	26.469236524911622	24.11515792869989
62-63	22.70448449464921	26.76688195802017	26.574977823439262	23.953655723891355
64-65	22.787637170857735	26.866858323805616	26.35695933396332	23.98854517137333
66-67	22.702306694015217	26.84761983885592	26.455626563408213	23.994446903720647
68-69	22.785299020776236	26.93877368208057	26.423380629375394	23.8525466677678
70-71	22.959598794403558	26.888919869066818	26.269655712508712	23.881825624020916
72-73	22.73861176624218	26.914229159839536	26.354993335261977	23.99216573865631
74-75	22.812658207300014	26.937916332845656	26.368683485101624	23.880741974752702
76-77	22.91025761670392	26.89583729960824	26.243332009155978	23.950573074531864
78-79	22.667929329959303	26.815551343493997	26.454059540751697	24.062459785795003
80-81	22.81317443033582	26.90264014831612	26.379273873974928	23.904911547373136
82-83	22.89389250839717	26.907446611509606	26.220455211824355	23.978205668268867
84-85	22.830205437599766	26.821158715044604	26.343154131558066	24.00548171579756
86-87	22.88799868761273	26.834986912479152	26.365460561185	23.91155383872312
88-89	23.02355173607392	26.734952005267637	26.24863644814413	23.992859810514318
90-91	22.840564972227995	26.725629589869648	26.421241482918223	24.012563954984135
92-93	22.95212461055807	26.81091528365534	26.395139220351894	23.841820885434693
94-95	23.024023843920478	26.752039727789466	26.291353316482624	23.932583111807432
96-97	22.864812273578288	26.627460149942312	26.390828793748973	24.11689878273043
98-99	23.019746083017687	25.8491554232993	26.867696746611475	24.263401747071537
100-101	23.757894520329927	12.28758167376772	33.29989992102855	30.654623884873804
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	56698.0
1	28451.0
2	158.0
3	101.0
4	90.0
5	91.5
6	105.0
7	116.0
8	131.0
9	170.0
10	222.0
11	334.5
12	544.0
13	805.5
14	1203.5
15	1821.5
16	2693.0
17	3863.5
18	5311.0
19	7205.0
20	9594.0
21	12471.0
22	16784.0
23	22556.5
24	30921.5
25	41610.5
26	54696.0
27	75396.5
28	105277.5
29	143063.5
30	194981.0
31	264768.5
32	364717.5
33	489906.0
34	654563.0
35	883644.5
36	1173112.5
37	1520982.5
38	1960320.0
39	2472439.5
40	2992079.0
41	3514808.0
42	4058462.5
43	4531030.0
44	4900769.0
45	5150044.5
46	5246134.0
47	5237279.0
48	5046468.5
49	4674087.0
50	4254191.0
51	3839744.0
52	3418309.5
53	2978937.5
54	2523347.5
55	2143982.0
56	1823260.5
57	1503607.5
58	1219241.0
59	1001128.0
60	841544.0
61	707087.0
62	602455.5
63	517979.5
64	457596.5
65	406204.5
66	357299.5
67	307165.0
68	236422.5
69	174092.5
70	135561.5
71	101708.5
72	74885.0
73	56970.0
74	44722.0
75	31948.0
76	17494.0
77	10814.0
78	7642.5
79	5946.0
80	4203.5
81	2557.0
82	1382.5
83	571.0
84	433.0
85	351.5
86	292.0
87	242.5
88	210.5
89	190.5
90	152.5
91	120.5
92	113.5
93	108.0
94	123.0
95	146.0
96	270.0
97	691.0
98	2071.0
99	2357.0
100	846.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1019364847905052
2	0.07599060901590969
3	0.06735833939105103
4	0.06559076273082486
5	0.0656199692189317
6	0.06558725795225204
7	0.06558725795225204
8	0.06558725795225204
9	0.06558842621177632
10-11	0.06558725795225204
12-13	0.06560828662368898
14-15	0.06568188697371821
16-17	0.06559368337963555
18-19	0.06577885251423293
20-21	0.06561120727249965
22-23	0.06580981139162619
24-25	0.06663810739433627
26-27	0.06750320357206098
28-29	0.06856865625819862
30-31	0.06940220942876794
32-33	0.07035784571962386
34-35	0.07140635864265954
36-37	1.262632122906762E-4
38-39	2.3966881557623765E-5
40-41	2.57208529161093E-5
42-43	3.478201721073838E-4
44-45	9.294816424686546E-5
46-47	1.8414509557890425E-4
48-49	1.3738002186166286E-4
50-51	6.138345648384419E-5
52-53	9.529201609873843E-5
54-55	4.033908968635411E-5
56-57	5.846359450923016E-7
58-59	1.1692922686073644E-6
60-61	0.0
62-63	8.185389531052837E-6
64-65	4.677473306098355E-6
66-67	8.185784422935204E-6
68-69	0.0
70-71	2.3389111217983757E-6
72-73	4.093208652872347E-6
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	6.550312720994466E-5
84-85	7.01851225016392E-6
86-87	9.943390290398703E-6
88-89	2.509383030628915E-4
90-91	4.679885834887041E-5
92-93	1.7552773481265145E-6
94-95	5.617638687956714E-4
96-97	1.933200121004268E-5
98-99	1.1330887253199112E-5
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
34-35	61631.0
36-37	869.0
38-39	951.0
40-41	983.0
42-43	1145.0
44-45	1152.0
46-47	1216.0
48-49	1206.0
50-51	1291.0
52-53	1613.0
54-55	1672.0
56-57	1506.0
58-59	1508.0
60-61	1933.0
62-63	1965.0
64-65	2139.0
66-67	2105.0
68-69	2121.0
70-71	2309.0
72-73	2544.0
74-75	2710.0
76-77	2861.0
78-79	3337.0
80-81	3664.0
82-83	3749.0
84-85	4271.0
86-87	4441.0
88-89	5761.0
90-91	14996.0
92-93	10486.0
94-95	32559.0
96-97	616667.0
98-99	7817018.0
100-101	7.698304E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	11.283106809740175
#Duplication Level	Percentage of deduplicated	Percentage of total
1	37.78376627141945	4.263182705146839
2	5.8187229022728175	1.3130654400525108
3	5.456208753363488	1.8468895842131856
4	5.464321863944352	2.4661810893473057
5	5.105962603224451	2.8805560709360236
6	4.786724152194715	3.240547192678558
7	4.148415415071709	3.276490995359871
8	3.8527611396325137	3.4776892360711944
9	3.2919666312545406	3.3429250003090973
>10	22.559360091277366	46.04491617837703
>50	1.1081232365981988	8.54840424276226
>100	0.5632440112873341	11.784476682667313
>500	0.04105782181246108	3.1649828105269133
>1k	0.018619171913512297	3.624022766124566
>5k	6.256226793985413E-4	0.4653040611455354
>10k+	1.203120537304887E-4	0.26036594428183735
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.1682595242737401E-6
2	0.0	0.0	0.0	9.346076194189921E-6	1.1682595242737401E-6
3	0.0	0.0	1.1682595242737401E-6	9.346076194189921E-6	1.1682595242737401E-6
4	0.0	0.0	2.3365190485474802E-6	9.346076194189921E-6	5.841297621368701E-6
5	0.0	0.0	2.3365190485474802E-6	9.346076194189921E-6	5.841297621368701E-6
6	1.401911429128488E-5	0.0	1.5187373815558622E-5	9.346076194189921E-6	5.841297621368701E-6
7	1.401911429128488E-5	0.0	2.2196930961201062E-5	9.346076194189921E-6	5.841297621368701E-6
8	1.75238928641061E-5	0.0	2.2196930961201062E-5	9.346076194189921E-6	5.841297621368701E-6
9	1.75238928641061E-5	0.0	2.2196930961201062E-5	1.75238928641061E-5	9.346076194189921E-6
10-11	2.803822858256976E-5	0.0	3.7384304776759684E-5	1.75238928641061E-5	1.635563333983236E-5
12-13	4.4977991684539E-5	1.1682595242737401E-6	5.2571678592318305E-5	1.75238928641061E-5	2.102867143692732E-5
14-15	6.892731193215067E-5	1.1682595242737401E-6	6.42542738350557E-5	1.75238928641061E-5	2.5701709534022282E-5
16-17	9.346076194189921E-5	1.75238928641061E-6	1.0981639528173158E-4	1.75238928641061E-5	2.5701709534022282E-5
18-19	1.0747987623318409E-4	2.3365190485474802E-6	1.2383550957301647E-4	4.088908334958091E-5	2.7454098820432894E-5
20-21	1.2792441790797455E-4	2.3365190485474802E-6	1.3434984529148012E-4	4.205734287385464E-5	2.9206488106843502E-5
22-23	1.384387536264382E-4	2.3365190485474802E-6	1.6822937149541857E-4	4.205734287385464E-5	2.9206488106843502E-5
24-25	1.4895308934490187E-4	5.25716785923183E-6	1.6822937149541857E-4	4.497799168453899E-5	2.9206488106843502E-5
26-27	1.7056589054396606E-4	8.76194643205305E-6	1.6822937149541857E-4	4.789864049522334E-5	2.9206488106843502E-5
28-29	1.9393108102944086E-4	9.346076194189921E-6	1.6822937149541857E-4	4.789864049522334E-5	3.563191549034907E-5
30-31	2.0619780603431512E-4	9.346076194189921E-6	1.6822937149541857E-4	5.023515954377082E-5	5.08192893059077E-5
32-33	2.2664234770910558E-4	9.346076194189921E-6	1.6822937149541857E-4	5.2571678592318305E-5	5.9581235737960745E-5
34-35	2.4358211081107483E-4	9.346076194189921E-6	1.9393108102944086E-4	5.2571678592318305E-5	6.659079288360319E-5
36-37	2.564329655780859E-4	9.346076194189921E-6	2.009406381750833E-4	5.2571678592318305E-5	6.659079288360319E-5
38-39	3.037474763111724E-4	9.346076194189921E-6	2.0327715722363076E-4	5.2571678592318305E-5	7.067970121856128E-5
40-41	3.644969715734069E-4	9.346076194189921E-6	2.2196930961201062E-4	5.2571678592318305E-5	7.476860955351937E-5
42-43	3.878621620588817E-4	2.453345000974854E-5	2.3131538580620055E-4	5.2571678592318305E-5	8.060990717488807E-5
44-45	4.1823690968999895E-4	2.5701709534022282E-5	2.3657255366543237E-4	5.2571678592318305E-5	8.878772384480425E-5
46-47	4.5737360375316927E-4	2.6869969058296023E-5	2.4883927867030666E-4	5.2571678592318305E-5	8.878772384480425E-5
48-49	4.900848704328339E-4	2.6869969058296023E-5	2.6753143105868646E-4	5.2571678592318305E-5	8.878772384480425E-5
50-51	5.356469918795098E-4	3.2711266679664726E-5	2.733727286800552E-4	5.2571678592318305E-5	9.579728099044668E-5
52-53	6.06326693098071E-4	3.7384304776759684E-5	2.873918429713401E-4	6.42542738350557E-5	1.0222270837395225E-4
54-55	6.542253335932944E-4	3.7384304776759684E-5	3.084205144082674E-4	7.59368690777931E-5	1.092322655195947E-4
56-57	6.986191955156966E-4	5.1403419068044564E-5	3.317857048937422E-4	7.59368690777931E-5	1.2967680719438513E-4
58-59	7.511908741080148E-4	8.528294527198303E-5	3.4230004061220584E-4	7.652099883992998E-5	1.425276619613963E-4
60-61	8.066832015110176E-4	8.528294527198303E-5	3.9311932991811354E-4	7.710512860206685E-5	1.425276619613963E-4
62-63	8.621755289140202E-4	8.528294527198303E-5	4.637990311366748E-4	7.710512860206685E-5	1.4369592148567002E-4
64-65	9.147472075063385E-4	8.937185360694112E-5	5.058563740105294E-4	7.710512860206685E-5	1.501213488691756E-4
66-67	9.714077944336149E-4	9.346076194189921E-5	5.093611525833506E-4	7.710512860206685E-5	1.5829916553909178E-4
68-69	0.0010146333968317433	9.346076194189921E-5	5.210437478260881E-4	7.710512860206685E-5	1.6005155482550238E-4
70-71	0.001068957464710472	9.346076194189921E-5	5.210437478260881E-4	7.710512860206685E-5	1.6238807387404985E-4
72-73	0.001132627608783391	9.346076194189921E-5	5.292215644960043E-4	7.710512860206685E-5	1.6472459292259735E-4
74-75	0.0012301772790602483	9.346076194189921E-5	5.292215644960043E-4	7.710512860206685E-5	1.6472459292259735E-4
76-77	0.0012856696064632508	9.521315122830982E-5	5.30389824020278E-4	7.710512860206685E-5	1.6472459292259735E-4
78-79	0.0013335682469584743	9.696554051472043E-5	5.30389824020278E-4	7.827338812634058E-5	1.6472459292259735E-4
80-81	0.0013767938493566027	9.696554051472043E-5	5.30389824020278E-4	8.002577741275119E-5	1.75238928641061E-4
82-83	0.0014194353219925942	9.696554051472043E-5	5.30389824020278E-4	8.17781666991618E-5	1.75238928641061E-4
84-85	0.0014831054660655132	9.696554051472043E-5	5.444089383115629E-4	8.17781666991618E-5	1.75238928641061E-4
86-87	0.0015164008625073145	9.696554051472043E-5	5.490819764086578E-4	8.17781666991618E-5	1.7640718816533475E-4
88-89	0.0015339247553714207	9.696554051472043E-5	5.490819764086578E-4	8.17781666991618E-5	1.7874370721388224E-4
90	0.0015339247553714207	9.696554051472043E-5	5.490819764086578E-4	8.17781666991618E-5	1.7874370721388224E-4
>>END_MODULE
