##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	SRR21290290_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	86029065
Sequences flagged as poor quality	0
Sequence length	35-101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.16194748832851	37.0	37.0	37.0	37.0	37.0
2	36.13938134745507	37.0	37.0	37.0	37.0	37.0
3	36.24376728957824	37.0	37.0	37.0	37.0	37.0
4	36.21360332115663	37.0	37.0	37.0	37.0	37.0
5	36.17764764733872	37.0	37.0	37.0	37.0	37.0
6	36.09818151574703	37.0	37.0	37.0	37.0	37.0
7	36.060869625864235	37.0	37.0	37.0	37.0	37.0
8	36.13850729401744	37.0	37.0	37.0	37.0	37.0
9	36.08936231028432	37.0	37.0	37.0	37.0	37.0
10-11	36.1144495409778	37.0	37.0	37.0	37.0	37.0
12-13	36.098090935894746	37.0	37.0	37.0	37.0	37.0
14-15	36.08718921913193	37.0	37.0	37.0	37.0	37.0
16-17	36.07448033987118	37.0	37.0	37.0	37.0	37.0
18-19	36.08039878150483	37.0	37.0	37.0	37.0	37.0
20-21	36.07038107411722	37.0	37.0	37.0	37.0	37.0
22-23	36.04379486746718	37.0	37.0	37.0	37.0	37.0
24-25	36.02903841277364	37.0	37.0	37.0	37.0	37.0
26-27	35.998238310505876	37.0	37.0	37.0	37.0	37.0
28-29	35.98117460651235	37.0	37.0	37.0	37.0	37.0
30-31	35.96271474646389	37.0	37.0	37.0	37.0	37.0
32-33	35.941440192334994	37.0	37.0	37.0	37.0	37.0
34-35	35.92466423411669	37.0	37.0	37.0	37.0	37.0
36-37	35.914970300988074	37.0	37.0	37.0	37.0	37.0
38-39	35.90451508504317	37.0	37.0	37.0	37.0	37.0
40-41	35.90703050299356	37.0	37.0	37.0	37.0	37.0
42-43	35.86889389137943	37.0	37.0	37.0	37.0	37.0
44-45	35.85635906933048	37.0	37.0	37.0	37.0	37.0
46-47	35.85933183459831	37.0	37.0	37.0	37.0	37.0
48-49	35.8383526852835	37.0	37.0	37.0	37.0	37.0
50-51	35.821484058006504	37.0	37.0	37.0	37.0	37.0
52-53	35.81011902724525	37.0	37.0	37.0	37.0	37.0
54-55	35.805438999195815	37.0	37.0	37.0	37.0	37.0
56-57	35.772897795800574	37.0	37.0	37.0	37.0	37.0
58-59	35.794278085011115	37.0	37.0	37.0	37.0	37.0
60-61	35.7624871699563	37.0	37.0	37.0	37.0	37.0
62-63	35.751241254995065	37.0	37.0	37.0	37.0	37.0
64-65	35.73774821104911	37.0	37.0	37.0	37.0	37.0
66-67	35.719806551007665	37.0	37.0	37.0	37.0	37.0
68-69	35.68551867994654	37.0	37.0	37.0	37.0	37.0
70-71	35.69401232492171	37.0	37.0	37.0	37.0	37.0
72-73	35.673313885940516	37.0	37.0	37.0	37.0	37.0
74-75	35.68469586352434	37.0	37.0	37.0	37.0	37.0
76-77	35.66076823394567	37.0	37.0	37.0	37.0	37.0
78-79	35.61604715989535	37.0	37.0	37.0	37.0	37.0
80-81	35.61368217401838	37.0	37.0	37.0	37.0	37.0
82-83	35.58298391164878	37.0	37.0	37.0	37.0	37.0
84-85	35.54207730306089	37.0	37.0	37.0	37.0	37.0
86-87	35.50712322459475	37.0	37.0	37.0	37.0	37.0
88-89	35.53468156557804	37.0	37.0	37.0	37.0	37.0
90-91	35.47855605158826	37.0	37.0	37.0	37.0	37.0
92-93	35.52138914319646	37.0	37.0	37.0	37.0	37.0
94-95	35.47673117099135	37.0	37.0	37.0	37.0	37.0
96-97	35.45475791046212	37.0	37.0	37.0	37.0	37.0
98-99	35.451682274115626	37.0	37.0	37.0	37.0	37.0
100-101	35.437116042686895	37.0	37.0	37.0	37.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	17828.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	49.0
12	2714.0
13	18702.0
14	35194.0
15	36158.0
16	32473.0
17	34139.0
18	37503.0
19	41913.0
20	48598.0
21	64921.0
22	89948.0
23	120047.0
24	149109.0
25	184436.0
26	225218.0
27	294086.0
28	382384.0
29	516430.0
30	696260.0
31	1004293.0
32	1476682.0
33	2464098.0
34	4609913.0
35	1.2119353E7
36	5.0926492E7
37	1.0400124E7
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.629076824201206	16.18176985060935	11.896627866337111	46.29252545885233
2	22.422880103227044	19.831475799347423	32.313684173046695	25.431959924378837
3	23.82098608402786	20.297577552141295	23.463796056575408	32.41764030725543
4	25.147613607432245	25.45505325604714	20.833706252042912	28.5636268844777
5	26.226769422691632	29.35777670419071	23.139929889904543	21.275523983213116
6	21.618188326947053	33.787853931681575	23.046460122202227	21.547497619169146
7	19.136660632997117	21.692024654129753	38.3087166444279	20.862598068445227
8	20.158782221319903	23.998289885935854	29.301479304240008	26.541448588504235
9	21.119555821753337	22.10243787218684	31.53882476470865	25.23918154135118
10-11	23.061204955084573	30.416004165782773	23.887426366280692	22.635364512851964
12-13	22.333819942353912	25.67446044289422	27.509669945310794	24.482049669441075
14-15	22.376614796498945	26.244972676531464	26.977557871080197	24.400854655889393
16-17	23.17681193652722	26.26461076363988	26.381846481330406	24.176730818502495
18-19	22.937318511245216	26.66604818119988	25.915875962315273	24.480757345239628
20-21	22.956864148563362	26.496689523847085	26.2249824418199	24.321463885769656
22-23	22.8327234538033	26.628469904870073	26.36351478611847	24.175291855208158
24-25	22.817012018460424	26.43631625722223	26.445171215282244	24.3015005090351
26-27	22.94259258183756	26.67427506761704	26.29442823428445	24.088704116260953
28-29	22.795486038339373	26.483535266124854	26.32093753479115	24.400041160744625
30-31	22.841286039843236	26.213635587998922	26.458765453381506	24.486312918776335
32-33	22.993123148774345	26.433486538228568	26.328436719665916	24.24495359333117
34-35	22.97934441767795	26.482838619922543	26.182055822781543	24.355761139617965
36-37	22.743779691519627	26.402109627912306	26.376625232682755	24.47748544788531
38-39	23.08345492057618	26.528977602922854	26.388755742950615	23.998811733550347
40-41	23.22238196208618	26.418042286215847	26.013206970999597	24.34636878069837
42-43	22.984816647022086	26.460247128949348	26.228531722842273	24.326404501186293
44-45	23.113108180475606	26.408389505488213	26.365303850159954	24.11319846387623
46-47	23.06027582676284	26.215616717906777	26.35320150998982	24.370905945340564
48-49	22.86522690621956	26.055766253831038	26.492330671997834	24.58667616795157
50-51	23.115482883076453	26.077189593440806	26.366039195630268	24.441288327852465
52-53	23.355994075039497	26.324655173388322	26.04450968290864	24.274841068663545
54-55	22.87347795701833	26.27967460665273	26.382715693722858	24.46413174260608
56-57	23.038807122278975	26.422411560969216	26.27926372222355	24.259517594528262
58-59	23.07523994660029	26.33304298451762	26.198154974504735	24.393562094377362
60-61	23.11374544142206	26.198994642093076	26.188047343831506	24.49921257265336
62-63	23.243334776939424	26.06642786750205	26.288293686042437	24.401943669516093
64-65	23.260081179720608	26.28739088564545	26.0277129286287	24.424815006005247
66-67	23.300139740893407	26.187060843695757	26.100962116461147	24.411837298949692
68-69	23.24996080731134	26.317334112212286	26.256645206587898	24.176059873888473
70-71	23.546289147185437	26.25485798902052	26.08897772110173	24.109875142692314
72-73	23.28394685172951	26.15377263225384	26.111266216724218	24.45101429929243
74-75	23.34189482897735	26.338316704738713	26.131963756172883	24.18782471011106
76-77	23.371547972387503	26.29689822428593	26.073902615406524	24.257651187920043
78-79	23.202935467220847	26.31779677263186	25.98845756077691	24.490810199370383
80-81	23.409045708902187	26.34266448618922	25.993428421682673	24.25486138322592
82-83	23.476498013818333	26.377067921129854	25.76721764688087	24.379216418170945
84-85	23.343843414060746	26.396616831910062	25.90025395019332	24.359285803835874
86-87	23.36723712531987	26.353702052453176	25.931378850693363	24.347681971533593
88-89	23.534534524015843	26.11946908993404	25.85654078928331	24.489455596766803
90-91	23.43507432296193	26.11922148007161	26.11927967006229	24.326424526904177
92-93	23.70399847997924	26.096362027349045	26.048322745937735	24.15131674673398
94-95	23.543337846320547	26.173797940230376	26.044692222682723	24.238171990766347
96-97	23.544455777763737	25.96140226134675	26.096861692564104	24.397280268325407
98-99	23.727974521608065	25.164046545613616	26.522859646483326	24.585119286294994
100-101	24.657411554896683	11.714226520445376	32.46545129028056	31.16291063437738
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	17911.0
1	9039.5
2	298.0
3	720.0
4	1262.0
5	1774.0
6	2251.5
7	2712.0
8	3167.0
9	3543.5
10	3867.5
11	4227.5
12	4537.0
13	4899.0
14	5391.0
15	5833.5
16	6359.0
17	7015.0
18	7831.0
19	8893.5
20	10126.0
21	12048.0
22	15307.5
23	19995.0
24	25599.5
25	32571.0
26	41893.5
27	56706.0
28	75622.5
29	99514.5
30	137030.0
31	185724.5
32	265905.5
33	379273.5
34	511404.0
35	676663.5
36	921921.5
37	1251444.0
38	1666026.5
39	2223366.5
40	2765624.0
41	3246503.0
42	3766225.5
43	4292206.0
44	4714262.0
45	5012738.0
46	5174595.0
47	5195118.5
48	5055430.5
49	4813059.5
50	4507239.0
51	4166078.5
52	3726553.5
53	3152079.0
54	2665645.5
55	2318341.5
56	2022377.0
57	1710210.0
58	1419444.0
59	1137022.5
60	934762.5
61	831585.5
62	774754.0
63	708892.5
64	603140.0
65	511568.5
66	452494.5
67	399162.5
68	324876.5
69	247140.0
70	183096.5
71	136221.5
72	103534.0
73	81144.0
74	68009.5
75	50918.5
76	33433.5
77	27354.0
78	21398.0
79	16380.5
80	14508.5
81	11450.5
82	8226.5
83	6307.0
84	6066.5
85	5950.0
86	5646.0
87	5506.0
88	5383.5
89	4956.5
90	4538.0
91	4121.5
92	3708.5
93	3288.5
94	2839.5
95	2414.0
96	2128.5
97	1919.5
98	1869.0
99	2185.5
100	10397.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.06728888661058911
2	0.03655276272036666
3	0.021556668086535637
4	0.021345111678245023
5	0.021320701323442257
6	0.022288978730618542
7	0.020732528012480433
8	0.021420667538348814
9	0.020724391227546178
10-11	0.020759263162978697
12-13	0.020748220383425067
14-15	0.020737177603871434
16-17	0.021606651193988916
18-19	0.020785998313476963
20-21	0.020775536732847207
22-23	0.022774279832054434
24-25	0.024122661335445175
26-27	0.02652533768674575
28-29	0.028815842645738388
30-31	0.030790175390142855
32-33	0.03305917598895211
34-35	0.03483067030892408
36-37	1.9186517362149917E-4
38-39	5.197897384226168E-4
40-41	6.262027038386173E-4
42-43	0.0010762524700604703
44-45	8.907898349134436E-4
46-47	6.425214733583734E-4
48-49	6.093909671663229E-4
50-51	0.0010472682214872388
52-53	3.9833130931600226E-4
54-55	3.721719886311227E-5
56-57	9.653438588844995E-5
58-59	1.5701758153774716E-5
60-61	4.478015239430257E-5
62-63	4.071028324540145E-6
64-65	2.0937263705167874E-5
66-67	1.2795345434864732E-5
68-69	2.093843579903927E-5
70-71	1.919411975926735E-5
72-73	5.816581636386358E-7
74-75	9.888513984947239E-6
76-77	2.6758093098688745E-5
78-79	5.0609552503007113E-5
80-81	2.722554632116347E-4
82-83	0.0016161463092483278
84-85	1.7046458464442223E-4
86-87	0.0012834885866766519
88-89	5.63225514320608E-4
90-91	0.002312998628679847
92-93	9.690055908654468E-4
94-95	0.0011082262757551365
96-97	0.0018257115499524179
98-99	0.0017242139271352174
100-101	3.7591520371325015E-4
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
34-35	30810.0
36-37	1529.0
38-39	1796.0
40-41	1687.0
42-43	1707.0
44-45	1758.0
46-47	1835.0
48-49	1867.0
50-51	1912.0
52-53	1928.0
54-55	2016.0
56-57	2089.0
58-59	2007.0
60-61	2200.0
62-63	2229.0
64-65	2354.0
66-67	2420.0
68-69	2442.0
70-71	2640.0
72-73	2795.0
74-75	2991.0
76-77	3102.0
78-79	3366.0
80-81	3538.0
82-83	3465.0
84-85	3957.0
86-87	3957.0
88-89	4967.0
90-91	13956.0
92-93	9415.0
94-95	31257.0
96-97	621271.0
98-99	7749970.0
100-101	7.7503832E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.029192345673465
#Duplication Level	Percentage of deduplicated	Percentage of total
1	62.59551720120409	14.41524205603441
2	14.612500627660213	6.7302817521132265
3	6.984410151327037	4.82535974387954
4	3.930328860887258	3.6204919727649747
5	2.4850931006868984	2.861484350631233
6	1.6395247044581456	2.2654157864670044
7	1.1689712988115195	1.8844325420831551
8	0.8942613333169357	1.64752930018033
9	0.7121718549732509	1.4760668368218657
>10	4.230375640942901	18.309416721712456
>50	0.39355812693904046	6.287132405546511
>100	0.2902623807588909	13.306873944649897
>500	0.033672594589242225	5.386649419517309
>1k	0.026821301054672394	12.05689706889532
>5k	0.0020463181035142307	3.1804488380814595
>10k+	4.845042863473805E-4	1.746277260621241
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.162397847750641E-6	0.0	1.162397847750641E-6	0.0	8.136784934254487E-6
2	1.162397847750641E-6	0.0	1.162397847750641E-6	3.487193543251923E-6	1.162397847750641E-5
3	3.487193543251923E-6	0.0	1.162397847750641E-6	3.487193543251923E-6	1.7435967716259615E-5
4	9.299182782005128E-6	0.0	5.811989238753205E-6	3.487193543251923E-6	1.7435967716259615E-5
5	3.370953758476859E-5	0.0	8.136784934254487E-6	3.487193543251923E-6	2.092316125951154E-5
6	4.649591391002564E-5	0.0	1.3948774173007692E-5	4.649591391002564E-6	2.092316125951154E-5
7	4.649591391002564E-5	0.0	2.092316125951154E-5	4.649591391002564E-6	2.092316125951154E-5
8	5.579509669203077E-5	0.0	2.092316125951154E-5	4.649591391002564E-6	2.324795695501282E-5
9	5.695749453978141E-5	0.0	2.208555910726218E-5	1.162397847750641E-5	2.6735150498264744E-5
10-11	7.38122633321657E-5	0.0	2.6153951574389423E-5	1.2786376325257051E-5	2.9059946193766026E-5
12-13	9.008583320067468E-5	0.0	3.1965940813142625E-5	1.3367575249132372E-5	3.487193543251923E-5
14-15	1.0229101060205641E-4	0.0	4.649591391002564E-5	1.5111172020758333E-5	4.475231713839968E-5
16-17	1.1682098369893943E-4	0.0	6.683787624566186E-5	1.5111172020758333E-5	4.9983107453277564E-5
18-19	1.2612016648094455E-4	0.0	7.264986548441505E-5	1.6854768792384295E-5	5.347030099652949E-5
20-21	1.3832534388232628E-4	0.0	8.020545149479424E-5	2.150436018338686E-5	6.393188162628525E-5
22-23	1.5169291913145864E-4	0.0	9.066703212455E-5	2.382915587888814E-5	6.50942794740359E-5
24-25	1.7668447285809744E-4	5.811989238753205E-7	9.124823104842531E-5	2.441035480276346E-5	6.50942794740359E-5
26-27	2.1388120398611795E-4	5.230790314877885E-6	9.182942997230063E-5	2.4991553726638782E-5	6.625667732178655E-5
28-29	2.627019135916449E-4	8.136784934254487E-6	9.706022028717852E-5	2.5572752650514103E-5	7.323106440829038E-5
30-31	3.1384741889267306E-4	8.136784934254487E-6	1.0287220952593173E-4	2.8478747269890705E-5	8.078665041866955E-5
32-33	3.6150573065044935E-4	8.136784934254487E-6	1.0868419876468493E-4	3.0222344041516667E-5	8.543624180967211E-5
34-35	4.085828434843503E-4	8.136784934254487E-6	1.325133546435731E-4	3.0222344041516667E-5	9.066703212455E-5
36-37	4.707711283390096E-4	9.299182782005128E-6	1.4123133850170287E-4	3.0222344041516667E-5	9.880381705880448E-5
38-39	5.474893862905519E-4	9.299182782005128E-6	1.4355613419720416E-4	3.0222344041516667E-5	1.1507738692731345E-4
40-41	6.224640474704682E-4	9.880381705880449E-6	1.4936812343595735E-4	3.1965940813142625E-5	1.2670136540481988E-4
42-43	7.009259021936366E-4	1.046158062975577E-5	1.5692370944633653E-4	3.370953758476859E-5	1.3135095679582244E-4
44-45	7.915929343181864E-4	1.046158062975577E-5	1.5924850514183783E-4	3.5453134356394555E-5	1.36000548186825E-4
46-47	8.74123181508482E-4	1.2786376325257051E-5	1.6680409115221698E-4	3.603433328026987E-5	1.389065428062016E-4
48-49	9.799013856537904E-4	1.3948774173007692E-5	1.6854768792384296E-4	3.9521526823521795E-5	1.452997309688301E-4
50-51	0.0010723120145499664	1.5692370944633654E-5	1.7668447285809744E-4	4.0683924671272436E-5	1.5808610729408718E-4
52-53	0.0011722782294565216	1.6273569868508974E-5	1.9121444595498045E-4	4.0683924671272436E-5	1.5808610729408718E-4
54-55	0.0012815436271450817	1.6273569868508974E-5	2.074880158234894E-4	4.0683924671272436E-5	1.673852900760923E-4
56-57	0.0013977834119201457	1.6273569868508974E-5	2.26667580311375E-4	4.0683924671272436E-5	1.952828384221077E-4
58-59	0.0015297155676398436	1.6273569868508974E-5	2.324795695501282E-4	4.126512359514775E-5	2.127188061383673E-4
60-61	0.001666878513674419	1.6273569868508974E-5	2.5165913403801376E-4	4.184632251902308E-5	2.1736839752936987E-4
62-63	0.0017866054919927353	1.6273569868508974E-5	2.7374469314527596E-4	4.24275214428984E-5	2.1969319322487113E-4
64-65	0.0019156316530930563	1.7435967716259615E-5	2.9059946193766027E-4	4.417111821452436E-5	2.3131717170237756E-4
66-67	0.002074298959311019	1.7435967716259615E-5	2.923430587092862E-4	4.5333516062275E-5	2.3945395663663204E-4
68-69	0.002213786701041096	1.9760763411760897E-5	2.9466785440478746E-4	4.82395106816516E-5	2.534027308096397E-4
70-71	0.0023364196739787887	1.9760763411760897E-5	2.9699265010028875E-4	5.2307903148778846E-5	2.8130027915565514E-4
72-73	0.0024741638189372398	1.9760763411760897E-5	3.097790264255458E-4	5.2307903148778846E-5	2.987362468719147E-4
74-75	0.0026740962487503497	1.9760763411760897E-5	3.1384741889267306E-4	5.2307903148778846E-5	3.0745423073004453E-4
76-77	0.0028095155980132993	1.9760763411760897E-5	3.1617221458817436E-4	5.2307903148778846E-5	3.167534135120497E-4
78-79	0.002893789441975221	1.9760763411760897E-5	3.179158113598003E-4	5.2307903148778846E-5	3.2779619306568073E-4
80-81	0.0030053796353592824	2.324795695501282E-5	3.214030049030522E-4	5.2307903148778846E-5	3.3535177907605997E-4
82-83	0.003079191898691448	2.324795695501282E-5	3.2430899952242884E-4	5.2307903148778846E-5	3.5569374141169617E-4
84-85	0.003173927323283125	2.324795695501282E-5	3.2663379521793014E-4	5.2307903148778846E-5	3.684801177369532E-4
86-87	0.0032087992587156444	2.4991553726638782E-5	3.277961930656808E-4	5.2307903148778846E-5	3.7603570374733237E-4
88-89	0.003215192446878273	2.5572752650514103E-5	3.30120988761182E-4	5.2307903148778846E-5	3.8707848330096344E-4
90	0.003215192446878273	2.6735150498264744E-5	3.324457844566833E-4	5.2307903148778846E-5	3.882408811487141E-4
>>END_MODULE
