##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	SRR21290299_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	76940795
Sequences flagged as poor quality	0
Sequence length	35-101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.076119177089346	37.0	37.0	37.0	37.0	37.0
2	36.047214159926476	37.0	37.0	37.0	37.0	37.0
3	36.156367931992385	37.0	37.0	37.0	37.0	37.0
4	36.130497338895445	37.0	37.0	37.0	37.0	37.0
5	36.1029512627209	37.0	37.0	37.0	37.0	37.0
6	36.02075125425985	37.0	37.0	37.0	37.0	37.0
7	35.975764040389755	37.0	37.0	37.0	37.0	37.0
8	36.05358344685677	37.0	37.0	37.0	37.0	37.0
9	35.99918004486437	37.0	37.0	37.0	37.0	37.0
10-11	36.03131176380489	37.0	37.0	37.0	37.0	37.0
12-13	36.01716389855343	37.0	37.0	37.0	37.0	37.0
14-15	36.00758149821561	37.0	37.0	37.0	37.0	37.0
16-17	35.991030402012356	37.0	37.0	37.0	37.0	37.0
18-19	35.99712798652523	37.0	37.0	37.0	37.0	37.0
20-21	35.98958308138095	37.0	37.0	37.0	37.0	37.0
22-23	35.9581835812848	37.0	37.0	37.0	37.0	37.0
24-25	35.94612876043196	37.0	37.0	37.0	37.0	37.0
26-27	35.91610208212691	37.0	37.0	37.0	37.0	37.0
28-29	35.89849202234004	37.0	37.0	37.0	37.0	37.0
30-31	35.87761547044062	37.0	37.0	37.0	37.0	37.0
32-33	35.852228417967346	37.0	37.0	37.0	37.0	37.0
34-35	35.8351929038425	37.0	37.0	37.0	37.0	37.0
36-37	35.82281202356788	37.0	37.0	37.0	37.0	37.0
38-39	35.80724005852768	37.0	37.0	37.0	37.0	37.0
40-41	35.80895675456721	37.0	37.0	37.0	37.0	37.0
42-43	35.77079662408619	37.0	37.0	37.0	37.0	37.0
44-45	35.75800855887066	37.0	37.0	37.0	37.0	37.0
46-47	35.759558675777875	37.0	37.0	37.0	37.0	37.0
48-49	35.73147521969048	37.0	37.0	37.0	37.0	37.0
50-51	35.71058322734572	37.0	37.0	37.0	37.0	37.0
52-53	35.69550506345172	37.0	37.0	37.0	37.0	37.0
54-55	35.69067290287187	37.0	37.0	37.0	37.0	37.0
56-57	35.65756823606969	37.0	37.0	37.0	37.0	37.0
58-59	35.68102192307881	37.0	37.0	37.0	37.0	37.0
60-61	35.64273586162973	37.0	37.0	37.0	37.0	37.0
62-63	35.62886456591791	37.0	37.0	37.0	37.0	37.0
64-65	35.61666857326237	37.0	37.0	37.0	37.0	37.0
66-67	35.5947720179328	37.0	37.0	37.0	37.0	37.0
68-69	35.55975086185538	37.0	37.0	37.0	37.0	37.0
70-71	35.56128428732092	37.0	37.0	37.0	37.0	37.0
72-73	35.53764250291865	37.0	37.0	37.0	37.0	37.0
74-75	35.550094638424206	37.0	37.0	37.0	37.0	37.0
76-77	35.52384260418704	37.0	37.0	37.0	37.0	37.0
78-79	35.475948590326276	37.0	37.0	37.0	37.0	37.0
80-81	35.47039856403748	37.0	37.0	37.0	37.0	37.0
82-83	35.43554701141984	37.0	37.0	37.0	37.0	37.0
84-85	35.388548527594	37.0	37.0	37.0	37.0	37.0
86-87	35.34773766882222	37.0	37.0	37.0	37.0	37.0
88-89	35.37308706662194	37.0	37.0	37.0	37.0	37.0
90-91	35.31091977792873	37.0	37.0	37.0	37.0	37.0
92-93	35.34918581314692	37.0	37.0	37.0	37.0	37.0
94-95	35.29647489463105	37.0	37.0	37.0	37.0	37.0
96-97	35.266744074443125	37.0	37.0	37.0	31.0	37.0
98-99	35.26130271938839	37.0	37.0	37.0	31.0	37.0
100-101	35.2432527112875	37.0	37.0	37.0	31.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	17654.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	40.0
12	1518.0
13	10697.0
14	20053.0
15	20348.0
16	17658.0
17	18099.0
18	20417.0
19	25597.0
20	35861.0
21	55371.0
22	83036.0
23	119855.0
24	156956.0
25	198614.0
26	242995.0
27	316116.0
28	409698.0
29	546358.0
30	731757.0
31	1046438.0
32	1532729.0
33	2555419.0
34	4768217.0
35	1.2285869E7
36	4.4088469E7
37	7614956.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.09278793311698	16.219993248290653	12.672943619897389	45.01427519869498
2	22.131696536970296	19.66677515151918	31.827324689295306	26.37420362221522
3	24.152124271043956	19.986290098066878	22.919997210179258	32.941588420709905
4	25.23434997385101	24.800306260832063	20.751985887332793	29.21335787798413
5	26.259565482106588	28.794677429197147	23.053509655501074	21.892247433195188
6	22.090134977042318	33.31176645631931	22.799639686815095	21.798458879823283
7	19.27147010268563	22.196561684372437	37.522074309768726	21.009893903173207
8	19.906265235691976	24.3999366063913	29.369147142891634	26.32465101502509
9	20.77179560719429	22.782962910327452	31.38966197660839	25.055579505869872
10-11	22.67732432991924	30.743809832144603	23.8927645624756	22.68610127546055
12-13	22.093801592496323	26.117050568756767	27.25498269837671	24.534165140370202
14-15	22.171217193069754	26.60835312955627	26.890209822250675	24.330219855123296
16-17	22.850664403504428	26.572478697605824	26.231620500629617	24.345236398260134
18-19	22.698306901131847	26.872175133053673	26.00604705281478	24.4234709129997
20-21	22.837972783076395	26.7858859553168	26.214014789706525	24.16212647190028
22-23	22.723587953845183	26.811904301278012	26.25248594862991	24.212021796246898
24-25	22.57227679435954	26.783169460916795	26.34147302857526	24.303080716148408
26-27	22.662009117604647	26.91751120284051	26.283598226609726	24.13688145294511
28-29	22.593713496290974	26.81660455978615	26.189753134697536	24.399928809225347
30-31	22.592901074030397	26.659451572682773	26.262275353297284	24.485371999989546
32-33	22.769442637545026	26.758972541148584	26.193281381740896	24.278303439565498
34-35	22.80999687330195	26.73001784069359	26.024016364301307	24.435968921703154
36-37	22.61401471455567	26.66831720691196	26.196637072900035	24.52103100563233
38-39	22.7777187752387	26.850846733100347	26.153984560653615	24.217449931007337
40-41	22.93783188916806	26.730515757436407	26.02836759455842	24.303284758837112
42-43	22.79632792681881	26.625653293772594	26.252921025374548	24.325097754034047
44-45	22.90526912777479	26.654024229018408	26.24892921932319	24.19177742388361
46-47	22.90539518459171	26.64990050216894	26.097584565028953	24.347119748210392
48-49	22.76556098786169	26.53087113619642	26.232664825425562	24.47090305051633
50-51	22.91930023985019	26.61503957774891	26.136240424242885	24.32941975815801
52-53	23.182830084469796	26.64104882030124	25.907738818835703	24.268382276393265
54-55	22.943696254511742	26.571013585975976	26.137266085198462	24.348024074313816
56-57	22.97030651625655	26.661848341326227	26.15950274372657	24.208342398690654
58-59	23.079474812897224	26.562809671869523	26.048762985132058	24.308952530101195
60-61	22.976685628284844	26.467148418519493	26.09123538683499	24.464930566360675
62-63	23.057509431276387	26.467921745630335	26.178618022923306	24.295950800169976
64-65	23.16317935518715	26.534347350935167	25.98652770622592	24.31594558765176
66-67	23.09993940981586	26.49581903273777	26.078372722850133	24.325868834596236
68-69	23.152508971590226	26.56808142939542	26.051872166759477	24.227537432254877
70-71	23.347534847066935	26.519590460206686	25.885073050313633	24.247801642412746
72-73	23.132170080175648	26.520283193531153	26.008001360905148	24.33954536538805
74-75	23.18324079054164	26.582836409882578	26.009129172523167	24.224793627052616
76-77	23.294926509725162	26.52987455390146	25.867474680788245	24.307724255585132
78-79	23.0947020196126	26.482594358104077	26.014955501515846	24.407748120767476
80-81	23.240496454154506	26.54763183586602	25.965952465923348	24.24591924405613
82-83	23.335511987504173	26.52490240368503	25.83691631563018	24.302669293180614
84-85	23.25183364839295	26.461800903055284	25.979447635060353	24.30691781349141
86-87	23.341027744719863	26.500900584538815	25.945583179005826	24.212488491735492
88-89	23.46042895022898	26.40207910864699	25.81954782302637	24.31794411809766
90-91	23.312555919729913	26.369046602387247	25.976943913272464	24.341453564610376
92-93	23.426130470418624	26.445772286418684	25.946437992343817	24.18165925081887
94-95	23.501510059412823	26.358614759159664	25.869153040791865	24.270722140635648
96-97	23.325703128160296	26.28680446713988	25.960968575039384	24.426523829660443
98-99	23.521765413459292	25.473306368691905	26.419240571547054	24.58568764630175
100-101	24.350841995142545	12.022224176150662	32.64210438349021	30.98482944521659
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	17761.0
1	8938.0
2	239.0
3	577.5
4	990.5
5	1397.0
6	1793.5
7	2101.0
8	2375.5
9	2622.5
10	2874.5
11	3215.5
12	3532.5
13	3751.5
14	4056.5
15	4561.5
16	5269.0
17	5992.5
18	6796.5
19	8123.5
20	9869.0
21	12003.0
22	14968.0
23	19469.5
24	25898.0
25	34329.5
26	45370.5
27	61784.5
28	85404.5
29	115618.0
30	157516.5
31	214342.0
32	292868.0
33	397355.5
34	536126.5
35	726872.0
36	967658.5
37	1257969.5
38	1630182.0
39	2075860.5
40	2534421.5
41	2985867.5
42	3457309.5
43	3876970.0
44	4209511.0
45	4435658.5
46	4538407.0
47	4532601.0
48	4389550.0
49	4117300.5
50	3776798.5
51	3439195.5
52	3102224.5
53	2733783.0
54	2351247.5
55	2034289.5
56	1749076.0
57	1461102.0
58	1217710.0
59	1033535.5
60	887055.5
61	774701.0
62	692683.5
63	621038.0
64	568025.5
65	515887.0
66	455863.0
67	397065.5
68	326325.0
69	257878.5
70	206325.0
71	158995.5
72	120130.5
73	93524.5
74	76859.0
75	56838.0
76	35570.0
77	26668.5
78	18437.0
79	11906.5
80	10560.5
81	8409.0
82	4822.0
83	2785.0
84	2518.5
85	2353.5
86	2193.5
87	2027.5
88	1927.0
89	1834.5
90	1727.0
91	1625.0
92	1472.0
93	1370.0
94	1306.0
95	1247.0
96	1179.5
97	1105.0
98	1198.5
99	1594.0
100	8599.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.06966265425253274
2	0.038866247742826154
3	0.02377152458588451
4	0.023560973083264866
5	0.023546676376296864
6	0.02446686442478272
7	0.02295921168997539
8	0.02367274733774196
9	0.022948814084907752
10-11	0.022977407498843756
12-13	0.022960511390608843
14-15	0.022954662737758296
16-17	0.023833910216290333
18-19	0.023008600314046664
20-21	0.0230047012121463
22-23	0.02495880111454528
24-25	0.02596671895578932
26-27	0.02833152425835995
28-29	0.030576107252336036
30-31	0.032736859555454294
32-33	0.03544543567557367
34-35	0.037674422261948295
36-37	2.073843629667905E-4
38-39	5.096961235919482E-4
40-41	6.71593423140869E-4
42-43	0.001075357091534337
44-45	8.61480462822674E-4
46-47	6.072791482596554E-4
48-49	5.929915807549523E-4
50-51	0.0010189076753125263
52-53	3.7584358352421376E-4
54-55	3.056267033567839E-5
56-57	9.169124859185652E-5
58-59	1.690818689336921E-5
60-61	4.552373117963596E-5
62-63	3.90217984350854E-6
64-65	1.4958951369184038E-5
66-67	8.455411645244307E-6
68-69	1.7561984667138533E-5
70-71	1.8863709342242295E-5
72-73	6.505033920499379E-7
74-75	4.553748133166557E-6
76-77	3.0576686322791886E-5
78-79	4.619282959896511E-5
80-81	2.706670457913857E-4
82-83	0.0015987299798355058
84-85	1.6463385716486287E-4
86-87	0.0012716116716094226
88-89	5.063415108962546E-4
90-91	0.002300265157956553
92-93	9.667868672453196E-4
94-95	0.0011297326650108368
96-97	0.0017888423034398203
98-99	0.0017976694320693105
100-101	3.473308645845879E-4
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
34-35	30071.0
36-37	1679.0
38-39	1908.0
40-41	1960.0
42-43	2132.0
44-45	2132.0
46-47	2149.0
48-49	2143.0
50-51	2315.0
52-53	2457.0
54-55	2771.0
56-57	2542.0
58-59	2873.0
60-61	2839.0
62-63	2979.0
64-65	3162.0
66-67	3281.0
68-69	3354.0
70-71	3536.0
72-73	3804.0
74-75	3793.0
76-77	4062.0
78-79	4488.0
80-81	4900.0
82-83	4960.0
84-85	5483.0
86-87	5714.0
88-89	7215.0
90-91	15771.0
92-93	11979.0
94-95	32952.0
96-97	557462.0
98-99	6994010.0
100-101	6.9207919E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.88054513758356
#Duplication Level	Percentage of deduplicated	Percentage of total
1	60.984336380762606	18.832295522917185
2	15.48959191658798	9.56654084685889
3	7.39870971834522	6.854285682517131
4	4.182273399003681	5.1660352990259275
5	2.605377006634405	4.022773112689804
6	1.781509811313379	3.300839648478646
7	1.2635032329921532	2.731236803252786
8	0.9602166160548203	2.37216100431509
9	0.7514351130893506	2.088425333494881
>10	4.103400012978694	23.06261325498848
>50	0.29327456860563483	6.225653672024075
>100	0.16804657436123066	9.83414229207939
>500	0.012670344234073537	2.667949223191867
>1k	0.005481904645005504	2.866379192340912
>5k	1.54133681643696E-4	0.306586244742789
>10k+	1.9266710205462E-5	0.10208286708200248
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.5994012669091863E-6	0.0	2.5994012669091863E-6	1.2997006334545932E-6	1.2997006334545932E-6
2	3.89910190036378E-6	6.498503167272966E-6	5.198802533818373E-6	7.79820380072756E-6	1.2997006334545932E-6
3	5.198802533818373E-6	9.097904434182152E-6	5.198802533818373E-6	7.79820380072756E-6	6.498503167272966E-6
4	1.8195808868364305E-5	9.097904434182152E-6	1.559640760145512E-5	1.169730570109134E-5	9.097904434182152E-6
5	2.339461140218268E-5	9.097904434182152E-6	2.4694312035637273E-5	1.169730570109134E-5	1.4296706968000526E-5
6	3.509191710327402E-5	9.097904434182152E-6	2.5994012669091866E-5	1.2997006334545933E-5	1.4296706968000526E-5
7	3.8991019003637795E-5	1.0397605067636745E-5	3.639161773672861E-5	1.2997006334545933E-5	1.4296706968000526E-5
8	4.418982153745617E-5	1.0397605067636745E-5	3.8991019003637795E-5	1.559640760145512E-5	1.559640760145512E-5
9	5.198802533818373E-5	1.0397605067636745E-5	4.289012090400158E-5	1.559640760145512E-5	1.9495509501818897E-5
10-11	5.848652850545669E-5	1.0397605067636745E-5	5.068832470472914E-5	1.559640760145512E-5	2.534416235236457E-5
12-13	7.60324870570937E-5	1.559640760145512E-5	6.368533103927506E-5	1.6246257918182417E-5	3.379221646981942E-5
14-15	9.357844560873072E-5	1.6246257918182417E-5	8.513039149127586E-5	2.0145359818546192E-5	4.0290719637092385E-5
16-17	1.2282170986145906E-4	1.9495509501818897E-5	1.1112440416036773E-4	2.2094910768728083E-5	4.353997122072887E-5
18-19	1.332193149290958E-4	2.339461140218268E-5	1.2282170986145906E-4	2.2094910768728083E-5	4.6139372487638065E-5
20-21	1.4816587221382364E-4	2.4044461718909974E-5	1.3776826714618687E-4	2.4044461718909974E-5	5.328772597163832E-5
22-23	1.6701153139891522E-4	2.5994012669091866E-5	1.4946557284727823E-4	2.534416235236457E-5	5.848652850545669E-5
24-25	2.1899955673709896E-4	3.379221646981942E-5	1.579136269647331E-4	2.7943563619273757E-5	5.848652850545669E-5
26-27	2.6123982732437326E-4	4.159042027054698E-5	1.722103339327336E-4	3.054296488618294E-5	5.978622913891129E-5
28-29	3.288242602640121E-4	4.548952217091076E-5	1.8260793900037035E-4	3.119281520291024E-5	6.953398388982073E-5
30-31	4.00957645420742E-4	4.9388624071274545E-5	1.878067415341887E-4	3.2492515836364834E-5	7.73321876905483E-5
32-33	4.6789222804365354E-4	5.003847438800184E-5	1.9365539438473439E-4	3.2492515836364834E-5	8.643009212473045E-5
34-35	5.406754635171108E-4	5.913637882218399E-5	2.2094910768728085E-4	3.379221646981942E-5	9.942709845927637E-5
36-37	6.368533103927507E-4	6.303548072254777E-5	2.4434371908946354E-4	3.639161773672861E-5	1.143736557440042E-4
38-39	7.369302591687544E-4	6.498503167272967E-5	2.60589977007646E-4	3.7691318370183205E-5	1.2542111112836824E-4
40-41	8.17511698442939E-4	6.693458262291155E-5	2.872338399934651E-4	3.8991019003637795E-5	1.332193149290958E-4
42-43	9.15639096268761E-4	7.73321876905483E-5	3.275245596305575E-4	4.224027058727428E-5	1.345190155625504E-4
44-45	0.0010339118539131288	8.513039149127586E-5	3.3727231438146694E-4	4.613937248763806E-5	1.4036766841309608E-4
46-47	0.0011703804204258612	9.032919402509422E-5	3.6456602768401337E-4	5.198802533818373E-5	1.4621632126364173E-4
48-49	0.0013010003340880477	9.227874497527612E-5	3.7691318370183205E-4	5.328772597163832E-5	1.53364674747642E-4
50-51	0.0014160238401487793	9.292859529200342E-5	3.9185974098655986E-4	5.523727692182021E-5	1.6051302823164227E-4
52-53	0.0015485933047611478	9.487814624218531E-5	4.1850360397237903E-4	5.78366781887294E-5	1.6766138171564254E-4
54-55	0.001724052890277518	9.487814624218531E-5	4.36049562524016E-4	6.238563040582047E-5	1.77409136466552E-4
56-57	0.0018871653197760694	9.812739782582178E-5	4.574946229760168E-4	6.563488198945696E-5	2.0795210135273492E-4
58-59	0.0020463786473742572	1.0137664940945828E-4	4.6854207836038086E-4	6.628473230618426E-5	2.2354850895419005E-4
60-61	0.0022185889813069906	1.0527575130982205E-4	5.068832470472913E-4	6.953398388982073E-5	2.30047012121463E-4
62-63	0.0023758527579549964	1.0527575130982205E-4	5.673193265029299E-4	7.018383420654804E-5	2.3394611402182677E-4
64-65	0.002550662493154639	1.0657545194327664E-4	5.965625907556583E-4	7.083368452327533E-5	2.449935694061908E-4
66-67	0.0027222229767706455	1.0787515257673124E-4	6.043607945563859E-4	7.408293610691182E-5	2.547413241571003E-4
68-69	0.0028996321132371975	1.0787515257673124E-4	6.173578008909318E-4	8.253099022436668E-5	2.6123982732437326E-4
70-71	0.00307704124970375	1.0917485321018583E-4	6.206070524745683E-4	8.448054117454856E-5	2.735869833421919E-4
72-73	0.003270696644088484	1.0917485321018583E-4	6.537494186276604E-4	8.578024180800315E-5	2.9243264252728346E-4
74-75	0.0035462331783808576	1.0917485321018583E-4	6.55049119261115E-4	8.837964307491233E-5	3.0218039727819297E-4
76-77	0.0037639330344845017	1.1632320669418609E-4	6.563488198945696E-4	9.487814624218531E-5	3.06729349495284E-4
78-79	0.0039056004035310527	1.2217185954473177E-4	6.576485205280242E-4	9.747754750909449E-5	3.1127830171237504E-4
80-81	0.004060264778912149	1.2477126081164095E-4	6.608977721116607E-4	1.0007694877600368E-4	3.275245596305575E-4
82-83	0.004181786788140154	1.29320213028732E-4	6.706455268625702E-4	1.0332620035964017E-4	3.444206678654672E-4
84-85	0.004316955654019431	1.3126976397891392E-4	6.803932816134796E-4	1.0527575130982205E-4	3.6001707546692234E-4
86-87	0.004389089039176161	1.338691652458231E-4	6.901410363643891E-4	1.0982470352691313E-4	3.756134830683774E-4
88-89	0.004400786344877253	1.35818716196005E-4	6.953398388982074E-4	1.1177425447709503E-4	3.9445914225346904E-4
90	0.004400786344877253	1.4296706968000526E-4	6.96639539531662E-4	1.1307395511054962E-4	4.055065976378331E-4
>>END_MODULE
