##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	SRR21290288_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	83113885
Sequences flagged as poor quality	0
Sequence length	35-101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.95086296351085	37.0	37.0	37.0	37.0	37.0
2	36.12926548915383	37.0	37.0	37.0	37.0	37.0
3	36.27115367065323	37.0	37.0	37.0	37.0	37.0
4	36.30731427125564	37.0	37.0	37.0	37.0	37.0
5	36.327845413555146	37.0	37.0	37.0	37.0	37.0
6	36.29162615849325	37.0	37.0	37.0	37.0	37.0
7	36.256498261391584	37.0	37.0	37.0	37.0	37.0
8	36.31440420574733	37.0	37.0	37.0	37.0	37.0
9	36.30080034160357	37.0	37.0	37.0	37.0	37.0
10-11	36.32100530494995	37.0	37.0	37.0	37.0	37.0
12-13	36.303770525225715	37.0	37.0	37.0	37.0	37.0
14-15	36.29943985893573	37.0	37.0	37.0	37.0	37.0
16-17	36.299707720316526	37.0	37.0	37.0	37.0	37.0
18-19	36.29456238268732	37.0	37.0	37.0	37.0	37.0
20-21	36.28729351900708	37.0	37.0	37.0	37.0	37.0
22-23	36.27328258689869	37.0	37.0	37.0	37.0	37.0
24-25	36.241232340420616	37.0	37.0	37.0	37.0	37.0
26-27	36.19437347923274	37.0	37.0	37.0	37.0	37.0
28-29	36.18196276109582	37.0	37.0	37.0	37.0	37.0
30-31	36.17098463752477	37.0	37.0	37.0	37.0	37.0
32-33	36.16395782726316	37.0	37.0	37.0	37.0	37.0
34-35	36.15047013504422	37.0	37.0	37.0	37.0	37.0
36-37	36.155781614899524	37.0	37.0	37.0	37.0	37.0
38-39	36.147893737333476	37.0	37.0	37.0	37.0	37.0
40-41	36.14731993654681	37.0	37.0	37.0	37.0	37.0
42-43	36.13103971978485	37.0	37.0	37.0	37.0	37.0
44-45	36.131144044000074	37.0	37.0	37.0	37.0	37.0
46-47	36.12311629056299	37.0	37.0	37.0	37.0	37.0
48-49	36.09345715477414	37.0	37.0	37.0	37.0	37.0
50-51	36.08913380042043	37.0	37.0	37.0	37.0	37.0
52-53	36.08976928162389	37.0	37.0	37.0	37.0	37.0
54-55	36.079364886427705	37.0	37.0	37.0	37.0	37.0
56-57	36.062161329853716	37.0	37.0	37.0	37.0	37.0
58-59	36.04982157619138	37.0	37.0	37.0	37.0	37.0
60-61	36.04650317131879	37.0	37.0	37.0	37.0	37.0
62-63	36.03661652090909	37.0	37.0	37.0	37.0	37.0
64-65	36.021149218767334	37.0	37.0	37.0	37.0	37.0
66-67	36.015291404214935	37.0	37.0	37.0	37.0	37.0
68-69	35.996185049017825	37.0	37.0	37.0	37.0	37.0
70-71	35.972413979761384	37.0	37.0	37.0	37.0	37.0
72-73	35.94993318974572	37.0	37.0	37.0	37.0	37.0
74-75	35.94900370312375	37.0	37.0	37.0	37.0	37.0
76-77	35.9335100460003	37.0	37.0	37.0	37.0	37.0
78-79	35.91684441429827	37.0	37.0	37.0	37.0	37.0
80-81	35.88486714943815	37.0	37.0	37.0	37.0	37.0
82-83	35.90079781495815	37.0	37.0	37.0	37.0	37.0
84-85	35.88600897129456	37.0	37.0	37.0	37.0	37.0
86-87	35.900136222009515	37.0	37.0	37.0	37.0	37.0
88-89	35.87127796029628	37.0	37.0	37.0	37.0	37.0
90-91	35.85299226079337	37.0	37.0	37.0	37.0	37.0
92-93	35.83863606490432	37.0	37.0	37.0	37.0	37.0
94-95	35.80979770418952	37.0	37.0	37.0	37.0	37.0
96-97	35.811017921784426	37.0	37.0	37.0	37.0	37.0
98-99	35.78462241695891	37.0	37.0	37.0	37.0	37.0
100-101	35.75612145606884	37.0	37.0	37.0	37.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	49588.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	3.0
15	70.0
16	425.0
17	1086.0
18	2057.0
19	3158.0
20	4698.0
21	7958.0
22	14101.0
23	27920.0
24	51303.0
25	91442.0
26	150893.0
27	248901.0
28	382894.0
29	566531.0
30	782145.0
31	1080213.0
32	1440494.0
33	2093761.0
34	3353903.0
35	7814009.0
36	4.9396076E7
37	1.5550256E7
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.192434220509536	15.991641890496828	11.313186218171024	47.50273767082261
2	21.11758497820962	18.831964811273146	33.29395675660121	26.756493453916026
3	23.167937216877736	19.353711424699423	23.010753798874163	34.467597559548686
4	24.55980935153448	24.57576687012794	20.90983908091531	29.95458469742227
5	25.80774260701984	28.498902115193452	23.260372961804155	22.432982315982553
6	21.73650973052839	32.81788564345521	23.406434174721298	22.0391704512951
7	18.960097862502828	22.26162463037519	37.520409039277126	21.257868467844858
8	19.35171497328148	24.127319105583954	30.064921876122057	26.456044045012504
9	20.345654643895923	22.435907692085806	31.98656939214209	25.231868271876184
10-11	22.306467603042496	30.55945745258038	24.25025098328347	22.883823961093658
12-13	21.77391132914332	26.08933179058438	27.376566785809132	24.760190094463166
14-15	21.86188066998814	26.540814801155594	27.001013565203415	24.596290963652855
16-17	22.66977214071447	26.442390268521045	26.27043009372102	24.617407497043466
18-19	22.531347555267928	26.69815863845831	25.975187750409674	24.79530605586409
20-21	22.685336498737605	26.55954431623469	26.29365382443642	24.461465360591287
22-23	22.540508188607152	26.60340904639915	26.352577962530034	24.503504802463667
24-25	22.38845168119664	26.566475468931515	26.419468974086936	24.62560387578491
26-27	22.477661813695352	26.688696115377148	26.4374116859182	24.396230385009304
28-29	22.38571061316487	26.613017431200568	26.31907845800507	24.68219349762949
30-31	22.406313189494167	26.441555686678292	26.364530360398714	24.787600763428827
32-33	22.63470542099368	26.53138993062235	26.249698368650755	24.584206279733213
34-35	22.65130822888314	26.517575335671328	26.09274189312238	24.738374542323154
36-37	22.487995387531605	26.420559982152664	26.253293883840534	24.8381507464752
38-39	22.61819504920567	26.620408777226178	26.276073428521805	24.485322745046346
40-41	22.778209171535995	26.501473901190064	26.131301791346733	24.589015135927205
42-43	22.67607966791886	26.378951945987257	26.31119704525244	24.63377134084144
44-45	22.76382666356758	26.382144631143273	26.360978165743877	24.49305053954527
46-47	22.741656143865992	26.40998770260098	26.180518543988196	24.667837609544833
48-49	22.644317668301436	26.262261029474963	26.278826308297702	24.814594993925894
50-51	22.78132612225152	26.342462032744905	26.219141497665944	24.657070347337626
52-53	23.047809840310105	26.413187574289505	25.95813410270806	24.580868482692335
54-55	22.804596998475805	26.346625676508268	26.161320177153524	24.687457147862403
56-57	22.783492023239212	26.46647150832025	26.228310190245786	24.521726278194752
58-59	22.881080047279816	26.378947467435687	26.118036360251335	24.621936125033162
60-61	22.83047446196384	26.23510432430579	26.136148001562248	24.798273212168127
62-63	22.923728878375936	26.172698671364213	26.274995789487406	24.62857666077245
64-65	23.0186129934121	26.323749723522265	26.04088393039936	24.61675335266627
66-67	23.019820426861568	26.27267783755546	26.06607134178045	24.641430393802526
68-69	23.02242318005357	26.359673728752416	26.08998899578842	24.5279140954056
70-71	23.259232852594874	26.28904705168286	25.92594743030559	24.525772665416678
72-73	23.062478112086442	26.284996365634356	26.0141073944191	24.638418127860103
74-75	23.102504967043416	26.339033595567336	26.02251108751861	24.535950349870642
76-77	23.192735744811223	26.300813371993293	25.885471495646016	24.620979387549465
78-79	22.968593625404626	26.258807652419357	26.046755414626865	24.72584330754915
80-81	23.11669830033595	26.344062676857327	25.969020626273643	24.570218396533082
82-83	23.16839637661173	26.325403579040273	25.864706977204154	24.641493067143838
84-85	23.174670470832	26.23672378668039	25.95857689448084	24.630028848006777
86-87	23.232957106991922	26.246912250343456	25.977410996205446	24.54271964645918
88-89	23.3286200947003	26.170687144595284	25.880619413100963	24.62007334760345
90-91	23.194437378776	26.113053971595544	26.0125158447301	24.67999280489835
92-93	23.29457211014837	26.200142149073276	26.01298631390902	24.492299426869344
94-95	23.369595324515398	26.154787862496576	25.920979035608973	24.55463777737905
96-97	23.214952474285134	26.076929743346067	25.96221676613217	24.745901016236633
98-99	23.41524816352215	25.30095741505347	26.42367372768607	24.86012069373831
100-101	24.170525857806002	11.912190860517217	32.60909058324548	31.3081926984313
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	49670.0
1	24863.0
2	64.0
3	66.5
4	74.0
5	98.0
6	110.5
7	115.0
8	115.5
9	128.5
10	163.0
11	211.0
12	294.5
13	431.5
14	630.0
15	896.0
16	1311.0
17	1936.0
18	2773.0
19	3775.5
20	5082.5
21	7145.0
22	9974.0
23	13964.5
24	20284.0
25	28020.5
26	38330.0
27	54831.5
28	78577.0
29	107723.0
30	146695.5
31	202743.0
32	284678.0
33	391770.5
34	531072.5
35	726008.0
36	980057.0
37	1286828.0
38	1681279.5
39	2166861.0
40	2671034.0
41	3164395.5
42	3689254.5
43	4153069.0
44	4518361.0
45	4780394.5
46	4930220.0
47	4986480.0
48	4848870.5
49	4531352.5
50	4168105.0
51	3809197.0
52	3439933.0
53	3039497.0
54	2613968.5
55	2267582.5
56	1955469.5
57	1624827.0
58	1346837.0
59	1137835.5
60	974919.0
61	846741.5
62	745752.5
63	666171.0
64	610367.0
65	552322.5
66	487531.5
67	428047.5
68	351122.5
69	271730.0
70	214725.5
71	162784.0
72	121287.5
73	94758.5
74	79143.5
75	57846.0
76	33853.5
77	25405.0
78	17190.0
79	10183.5
80	8670.0
81	6159.0
82	2567.0
83	631.0
84	422.5
85	310.5
86	240.0
87	207.5
88	157.5
89	117.5
90	113.5
91	102.5
92	88.5
93	83.5
94	97.0
95	143.5
96	281.0
97	753.0
98	2336.5
99	2782.5
100	1076.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0960092287828923
2	0.06947094339291203
3	0.06142415313638629
4	0.059667527272970096
5	0.05969520014616089
6	0.0596627145993717
7	0.0596627145993717
8	0.0596627145993717
9	0.0596627145993717
10-11	0.0596627145993717
12-13	0.0596813637095655
14-15	0.05975896807133971
16-17	0.0596699336097693
18-19	0.05986003421690611
20-21	0.0596933953935615
22-23	0.060418905938520374
24-25	0.06368250022243575
26-27	0.06643534952072087
28-29	0.0692170748605964
30-31	0.07144955382605445
32-33	0.07380716230507094
34-35	0.0762056785096738
36-37	1.26431517089402E-4
38-39	2.5888873239834325E-5
40-41	3.070607721434468E-5
42-43	3.4861151285728027E-4
44-45	9.09180568198116E-5
46-47	1.812378091983258E-4
48-49	1.4330743011391886E-4
50-51	5.6601613929989564E-5
52-53	1.0296910759372119E-4
54-55	3.492602301001065E-5
56-57	0.0
58-59	1.2044033589003075E-6
60-61	0.0
62-63	4.2156314010410665E-6
64-65	2.4089983121232873E-6
66-67	7.829470612813809E-6
68-69	0.0
70-71	1.2046021657096635E-6
72-73	3.613916767027645E-6
74-75	0.0
76-77	6.023576652479558E-7
78-79	0.0
80-81	6.024007368132085E-7
82-83	5.6627759339685624E-5
84-85	6.626919527906446E-6
86-87	8.434621896143489E-6
88-89	2.590755224970369E-4
90-91	4.398571242832935E-5
92-93	4.218573933861957E-6
94-95	5.991121290847234E-4
96-97	2.232661705849246E-5
98-99	1.780753746147094E-5
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
34-35	64579.0
36-37	1583.0
38-39	1842.0
40-41	1711.0
42-43	1921.0
44-45	1767.0
46-47	1801.0
48-49	1707.0
50-51	1845.0
52-53	2018.0
54-55	1945.0
56-57	1979.0
58-59	2133.0
60-61	2177.0
62-63	2222.0
64-65	2367.0
66-67	2377.0
68-69	2343.0
70-71	2487.0
72-73	2532.0
74-75	2713.0
76-77	2798.0
78-79	3020.0
80-81	3095.0
82-83	3190.0
84-85	3576.0
86-87	3714.0
88-89	4644.0
90-91	14512.0
92-93	9397.0
94-95	33055.0
96-97	594070.0
98-99	7511709.0
100-101	7.4821056E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.767653274554245
#Duplication Level	Percentage of deduplicated	Percentage of total
1	53.93755628212056	13.35906692472317
2	17.261586772365323	8.550579922931524
3	8.654334616705718	6.430426773256187
4	5.100174775967427	5.052774419631545
5	3.179302327030342	3.937192884543549
6	2.1675220434126516	3.2210660661718724
7	1.5445000566952276	2.677754932072974
8	1.1964162970405556	2.370593921370127
9	0.9005428391350576	2.007389951872181
>10	5.362300648650587	24.43624118073222
>50	0.41357929933978343	7.060931525377069
>100	0.25060333084887	11.85796044221361
>500	0.020436562076736556	3.4543607619079317
>1k	0.01070362284251591	4.655737698606915
>5k	3.513053598787309E-4	0.5643295117806715
>10k+	8.92204088580904E-5	0.3635930828083146
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	4.812673598400556E-6	0.0	8.422178797200973E-6
2	0.0	2.406336799200278E-6	4.812673598400556E-6	0.0	1.3234852395601528E-5
3	3.609505198800417E-6	6.015841998000695E-6	4.812673598400556E-6	0.0	3.489188358840403E-5
4	1.203168399600139E-5	9.625347196801112E-6	6.015841998000695E-6	0.0	3.970455718680459E-5
5	2.16570311928025E-5	9.625347196801112E-6	9.625347196801112E-6	0.0	4.572039918480528E-5
6	3.489188358840403E-5	9.625347196801112E-6	1.5641189194801808E-5	0.0	4.572039918480528E-5
7	3.970455718680459E-5	1.082851559640125E-5	2.6469704791203057E-5	0.0	4.572039918480528E-5
8	4.3314062385605E-5	1.4438020795201667E-5	2.8876041590403335E-5	0.0	4.812673598400556E-5
9	4.572039918480528E-5	1.8047525994002086E-5	3.248554678920376E-5	1.203168399600139E-6	5.173624118280598E-5
10-11	6.31663409790073E-5	1.8047525994002086E-5	3.8501388787204446E-5	3.0079209990003474E-6	6.076000417980702E-5
12-13	7.88075301738091E-5	2.0453862793202364E-5	5.71504989810066E-5	8.422178797200973E-6	7.459644077520862E-5
14-15	9.26439667692107E-5	2.0453862793202364E-5	8.001069857340924E-5	8.422178797200973E-6	8.121386697300938E-5
16-17	1.064804033646123E-4	2.1657031192802504E-5	9.384713516881083E-5	8.422178797200973E-6	8.662812477121E-5
18-19	1.2272317675921418E-4	2.286019959240264E-5	1.0828515596401251E-4	9.625347196801112E-6	8.783129317081014E-5
20-21	1.2934060295701497E-4	2.346178379220271E-5	1.323485239560153E-4	1.203168399600139E-5	9.144079836961057E-5
22-23	1.4317703955241655E-4	2.526653639160292E-5	1.5520872354841793E-4	1.203168399600139E-5	9.625347196801112E-5
24-25	1.636309023456189E-4	2.7672873190803194E-5	1.6062298134661857E-4	1.4438020795201667E-5	9.625347196801112E-5
26-27	1.81678428339621E-4	3.248554678920376E-5	1.6844357594401947E-4	1.8649110193802153E-5	9.625347196801112E-5
28-29	2.1596872772822496E-4	3.368871518880389E-5	1.8288159673922113E-4	2.16570311928025E-5	1.0166772976621175E-4
30-31	2.4905585871722876E-4	3.7298220387604306E-5	1.876942703376217E-4	2.286019959240264E-5	1.0347248236561196E-4
32-33	2.701113057102312E-4	3.8501388787204446E-5	1.9371011233562237E-4	2.286019959240264E-5	1.143009979620132E-4
34-35	2.965810105014343E-4	3.9102972987004517E-5	2.2439090652542594E-4	2.406336799200278E-5	1.2753585035761475E-4
36-37	3.3147289408983827E-4	4.090772558640473E-5	2.3943051152042766E-4	2.406336799200278E-5	1.4859129735061717E-4
38-39	3.663647776782423E-4	4.15093097862048E-5	2.568764533146297E-4	2.466495219180285E-5	1.672404075444193E-4
40-41	3.976471560678459E-4	4.271247818580494E-5	2.719160583096314E-4	2.526653639160292E-5	1.792720915404207E-4
42-43	4.3314062385605003E-4	4.752515178420549E-5	3.0019051570023467E-4	2.6469704791203057E-5	1.8288159673922113E-4
44-45	4.7043884424365437E-4	5.6548914781206536E-5	3.11620615496436E-4	2.7672873190803194E-5	1.9671803333462274E-4
46-47	5.035259752326582E-4	5.6548914781206536E-5	3.398950728870393E-4	3.1282378389603616E-5	2.0754654893102398E-4
48-49	5.54059048015864E-4	5.6548914781206536E-5	3.609505198800417E-4	3.729822038760431E-5	2.189766487272253E-4
50-51	5.931620210028685E-4	5.6548914781206536E-5	3.7358378807584317E-4	3.7899804587404376E-5	2.4364160091902814E-4
52-53	6.280539045912725E-4	5.775208318080667E-5	3.9042814567024514E-4	4.331406238560501E-5	2.5146219551642904E-4
54-55	6.74375887975878E-4	5.775208318080667E-5	4.1509309786204795E-4	4.6923567584405424E-5	2.604859585134301E-4
56-57	7.17689950361483E-4	6.076000417980702E-5	4.493833972506519E-4	4.6923567584405424E-5	2.6710338471123084E-4
58-59	7.736372809428894E-4	6.136158837960709E-5	4.638214180458536E-4	4.752515178420549E-5	2.791350687072323E-4
60-61	8.48835305917898E-4	6.136158837960709E-5	5.119481540298591E-4	4.812673598400556E-5	2.8154140550643253E-4
62-63	9.029778838999043E-4	6.256475677920723E-5	5.630828110128651E-4	4.812673598400556E-5	2.839477423056328E-4
64-65	9.480966988849095E-4	6.497109357840751E-5	5.943651894024687E-4	4.812673598400556E-5	2.94174673702234E-4
66-67	0.0010070519504653164	6.617426197800764E-5	5.979746946012691E-4	4.993148858340577E-5	3.007920999000348E-4
68-69	0.0010690151230447237	6.617426197800764E-5	6.045921207990699E-4	5.4142577982006254E-5	3.044016050988352E-4
70-71	0.0011285719588249306	6.617426197800764E-5	6.076000417980702E-4	5.6548914781206536E-5	3.092142786972357E-4
72-73	0.001202566815400339	6.617426197800764E-5	6.202333099938717E-4	5.835366738060674E-5	3.254570520918376E-4
74-75	0.001271748998377347	6.737743037760778E-5	6.220380625932718E-4	5.895525158040681E-5	3.3688715188803895E-4
76-77	0.0013252899921595532	7.339327237560848E-5	6.256475677920723E-4	5.955683578020688E-5	3.4831725168424024E-4
78-79	0.00138304207534036	7.339327237560848E-5	6.256475677920723E-4	6.015841998000695E-5	3.5914576728064153E-4
80-81	0.0014401925743213664	7.339327237560848E-5	6.292570729908727E-4	6.015841998000695E-5	3.669663618780424E-4
82-83	0.001479897131508171	7.579960917480875E-5	6.382808359878737E-4	6.015841998000695E-5	3.8140438267324407E-4
84-85	0.001530430204291377	7.640119337460882E-5	6.448982621856745E-4	6.015841998000695E-5	3.946392350688456E-4
86-87	0.0015562983248827798	8.061228277320931E-5	6.491093515842751E-4	6.015841998000695E-5	4.247184450588491E-4
88-89	0.0015617125826809805	8.061228277320931E-5	6.551251935822758E-4	6.497109357840751E-5	4.560008234484527E-4
90	0.0015617125826809805	8.061228277320931E-5	6.617426197800764E-4	6.497109357840751E-5	4.5961032864725313E-4
>>END_MODULE
