##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	SRR21290311_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	107618968
Sequences flagged as poor quality	0
Sequence length	35-101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.27883015009027	37.0	37.0	37.0	37.0	37.0
2	36.30386385976123	37.0	37.0	37.0	37.0	37.0
3	36.3856265653839	37.0	37.0	37.0	37.0	37.0
4	36.40642222103449	37.0	37.0	37.0	37.0	37.0
5	36.42077711616785	37.0	37.0	37.0	37.0	37.0
6	36.39420917881316	37.0	37.0	37.0	37.0	37.0
7	36.347026343906215	37.0	37.0	37.0	37.0	37.0
8	36.40294866050007	37.0	37.0	37.0	37.0	37.0
9	36.384956739224634	37.0	37.0	37.0	37.0	37.0
10-11	36.40435248830856	37.0	37.0	37.0	37.0	37.0
12-13	36.38158291482594	37.0	37.0	37.0	37.0	37.0
14-15	36.375154429096554	37.0	37.0	37.0	37.0	37.0
16-17	36.37308136052745	37.0	37.0	37.0	37.0	37.0
18-19	36.367064651651376	37.0	37.0	37.0	37.0	37.0
20-21	36.35969003623971	37.0	37.0	37.0	37.0	37.0
22-23	36.344501203542485	37.0	37.0	37.0	37.0	37.0
24-25	36.315937762941566	37.0	37.0	37.0	37.0	37.0
26-27	36.27260889548764	37.0	37.0	37.0	37.0	37.0
28-29	36.260453668353335	37.0	37.0	37.0	37.0	37.0
30-31	36.25115835528176	37.0	37.0	37.0	37.0	37.0
32-33	36.24420460434075	37.0	37.0	37.0	37.0	37.0
34-35	36.231211174595174	37.0	37.0	37.0	37.0	37.0
36-37	36.21981537164013	37.0	37.0	37.0	37.0	37.0
38-39	36.21205199632503	37.0	37.0	37.0	37.0	37.0
40-41	36.210621535038655	37.0	37.0	37.0	37.0	37.0
42-43	36.19489339582783	37.0	37.0	37.0	37.0	37.0
44-45	36.19494517024574	37.0	37.0	37.0	37.0	37.0
46-47	36.18879293530077	37.0	37.0	37.0	37.0	37.0
48-49	36.161623093364696	37.0	37.0	37.0	37.0	37.0
50-51	36.15682283661205	37.0	37.0	37.0	37.0	37.0
52-53	36.15730634991141	37.0	37.0	37.0	37.0	37.0
54-55	36.146683297039104	37.0	37.0	37.0	37.0	37.0
56-57	36.13152492368042	37.0	37.0	37.0	37.0	37.0
58-59	36.119764744655185	37.0	37.0	37.0	37.0	37.0
60-61	36.118295997429826	37.0	37.0	37.0	37.0	37.0
62-63	36.108909302686385	37.0	37.0	37.0	37.0	37.0
64-65	36.09364233494602	37.0	37.0	37.0	37.0	37.0
66-67	36.08723790603345	37.0	37.0	37.0	37.0	37.0
68-69	36.07038865791509	37.0	37.0	37.0	37.0	37.0
70-71	36.04860130504511	37.0	37.0	37.0	37.0	37.0
72-73	36.02876100586621	37.0	37.0	37.0	37.0	37.0
74-75	36.02652489773335	37.0	37.0	37.0	37.0	37.0
76-77	36.01311257250411	37.0	37.0	37.0	37.0	37.0
78-79	35.99847253648274	37.0	37.0	37.0	37.0	37.0
80-81	35.96947862405242	37.0	37.0	37.0	37.0	37.0
82-83	35.982800053631536	37.0	37.0	37.0	37.0	37.0
84-85	35.96797974634694	37.0	37.0	37.0	37.0	37.0
86-87	35.98084725580483	37.0	37.0	37.0	37.0	37.0
88-89	35.953866347298145	37.0	37.0	37.0	37.0	37.0
90-91	35.93617208632435	37.0	37.0	37.0	37.0	37.0
92-93	35.924262100523805	37.0	37.0	37.0	37.0	37.0
94-95	35.89895127344493	37.0	37.0	37.0	37.0	37.0
96-97	35.8986468113806	37.0	37.0	37.0	37.0	37.0
98-99	35.875164918993725	37.0	37.0	37.0	37.0	37.0
100-101	35.83025006307001	37.0	37.0	37.0	37.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	12993.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	1.0
14	37.0
15	406.0
16	2134.0
17	5820.0
18	9154.0
19	11827.0
20	14698.0
21	19092.0
22	26224.0
23	42368.0
24	72553.0
25	126831.0
26	205062.0
27	331188.0
28	498734.0
29	726464.0
30	973166.0
31	1302092.0
32	1669077.0
33	2309375.0
34	3542471.0
35	8187634.0
36	6.3544863E7
37	2.3984704E7
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.143378161653764	15.925880086905536	16.042765043907604	47.8879767075331
2	22.226858266088882	20.673489151559764	32.093925271816175	25.005727310535182
3	23.98288130314133	21.629372741167533	23.03773415083094	31.350011804860202
4	24.222513891282656	26.20869450984199	20.72244799345434	28.846343605421016
5	26.05262097740046	30.373069071601943	23.12177454507741	20.452535405920184
6	20.95417099282823	34.851057294913225	22.583809123982196	21.610962588276347
7	18.70195033314832	21.09874196112251	39.55260198144202	20.646705724287152
8	20.11618964467354	24.030794758376565	29.178827662683226	26.674187934266662
9	20.856792958881567	22.428146251695527	31.432608299541144	25.282452489881763
10-11	22.978539634897317	30.87842813040088	23.28751039748262	22.85552183721918
12-13	22.011206898302206	25.877568644423217	27.36734724687241	24.74387721040217
14-15	22.207364091241207	26.51015972617295	26.849775933475378	24.432700249110464
16-17	22.93627656177124	26.573119590285906	26.213140472399836	24.27746337554301
18-19	22.748461608682266	26.96499979754799	25.94381225858109	24.34272633518865
20-21	22.956269063567817	26.92505231089033	26.117437969118797	24.001240656423057
22-23	22.710003546463835	26.977317612070976	26.36212434614803	23.950554495317157
24-25	22.51120255676089	26.97814551196089	26.458646399931602	24.05200553134662
26-27	22.72783303054427	27.015119521879765	26.35499389688479	23.90205355069117
28-29	22.674970531697074	26.909837146125376	26.247158940550907	24.16803338162664
30-31	22.586813960685863	26.934757206332005	26.310968300192933	24.1674605327892
32-33	22.878448889194154	26.991620152657337	26.188970066588812	23.9409608915597
34-35	22.85549447890542	26.857546297968725	26.12745099724832	24.15950822587753
36-37	22.673513816213912	26.8084761821627	26.28177813773558	24.23623186388781
38-39	23.01498890429346	26.947249215075473	26.15102335848949	23.886738522141574
40-41	23.036501571946918	26.804059707518356	26.048551178408943	24.11088754212578
42-43	22.820360877489872	26.764993831297602	26.334910809856016	24.07973448135651
44-45	22.984571177620523	26.772463311805232	26.318385314529163	23.924580196045085
46-47	22.949153659422933	26.72477475553095	26.243025102393442	24.08304648265268
48-49	22.794762536946866	26.677294351058613	26.267445330421474	24.260497781573047
50-51	23.0718393489068	26.79680164345567	26.100729677356927	24.030629330280608
52-53	23.208336053972527	26.806946308872988	25.977803766820813	24.006913870333676
54-55	22.81122644320997	26.750052761101085	26.281787096558944	24.15693369913
56-57	22.973930479236156	26.842711565003196	26.241888521763013	23.941469433997632
58-59	23.063675000571074	26.675517546285572	26.21067440528389	24.050133047859468
60-61	22.995893367878708	26.62372895172973	26.20243088769618	24.17794679269538
62-63	23.10315591350397	26.6557034468562	26.270165099453298	23.970975540186533
64-65	23.120722039585775	26.73971100920009	26.134548667834068	24.005018283380068
66-67	23.05873296733127	26.676058276057375	26.247537512264856	24.017671244346506
68-69	23.249146533679056	26.75204083911675	26.124414962087155	23.87439766511704
70-71	23.33255543490809	26.715210596301326	26.01919858891503	23.93303537987555
72-73	23.099444069116227	26.74091719377769	26.15065591863673	24.00898281846935
74-75	23.172090199091187	26.73229491208946	26.25012512765136	23.845489761167997
76-77	23.229637490449342	26.637633072885208	26.162032043053767	23.970697393611683
78-79	23.036073450588304	26.67029473557747	26.247551402188968	24.046080411645253
80-81	23.31073592856884	26.773398295662314	26.09915554146004	23.8167102343088
82-83	23.316831720923563	26.76332388842797	26.008810648403795	23.911033742244673
84-85	23.14523562688203	26.75049459848415	26.135847261236663	23.968422513397158
86-87	23.27472450261732	26.705599488681486	26.165859919452522	23.853816089248667
88-89	23.399088998499266	26.513475406371427	26.144470138478898	23.942965456650406
90-91	23.20099469178901	26.575647620706505	26.267621285948174	23.95573640155631
92-93	23.34548880463153	26.679126703396015	26.190363209851817	23.785021282120635
94-95	23.364457996150858	26.605382434818235	26.174334798137068	23.855824770893836
96-97	23.237616190306344	26.49262990753874	26.3064593034258	23.963294598729114
98-99	23.47541900569684	25.65000338602463	26.716165356809775	24.158412251468764
100-101	24.17186347158343	12.118030115931024	33.11374767321452	30.59635873927103
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	13101.0
1	6572.5
2	43.5
3	41.0
4	43.5
5	50.5
6	57.0
7	62.5
8	77.0
9	105.0
10	146.0
11	227.5
12	338.5
13	518.5
14	778.0
15	1147.5
16	1758.0
17	2578.5
18	3572.5
19	4999.0
20	6870.5
21	9230.0
22	13015.5
23	19020.0
24	26954.5
25	36968.5
26	52098.5
27	76180.5
28	109405.5
29	149670.5
30	208982.5
31	291041.0
32	408872.5
33	562984.5
34	759953.5
35	1027541.0
36	1377346.0
37	1815216.0
38	2355144.5
39	2991417.5
40	3645525.0
41	4302322.0
42	4984843.5
43	5583710.0
44	6055588.5
45	6376647.0
46	6530412.0
47	6539472.0
48	6315393.5
49	5906714.0
50	5420443.5
51	4893312.0
52	4371220.5
53	3834833.5
54	3305570.0
55	2852843.5
56	2446538.5
57	2034438.0
58	1632238.5
59	1314546.0
60	1105994.0
61	959710.0
62	846073.0
63	736087.5
64	634098.0
65	554615.0
66	487190.0
67	422367.0
68	340588.5
69	254834.5
70	197564.0
71	154634.5
72	115217.0
73	87751.5
74	69382.0
75	52404.5
76	33233.0
77	22481.5
78	16065.0
79	11012.5
80	8632.5
81	5930.0
82	3169.0
83	1327.0
84	1076.0
85	889.0
86	741.5
87	618.0
88	514.0
89	453.0
90	402.5
91	370.0
92	354.5
93	347.5
94	394.5
95	614.0
96	1206.0
97	3164.0
98	10489.0
99	11840.0
100	3878.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04879901840352158
2	0.023078645392696946
3	0.013922266937181555
4	0.012074079729142172
5	0.012101026651732991
6	0.012073150524914901
7	0.012073150524914901
8	0.012073150524914901
9	0.01207500893336944
10-11	0.012073615127028536
12-13	0.012091270007346661
14-15	0.012163747937073695
16-17	0.012083371771414868
18-19	0.01228315068027785
20-21	0.012106137274982975
22-23	0.012256203757687026
24-25	0.013072045069229803
26-27	0.013848859803227252
28-29	0.014925807502632808
30-31	0.015668241680221278
32-33	0.016524503375650285
34-35	0.017503420029078887
36-37	1.4962937431310764E-4
38-39	3.5781346363493213E-5
40-41	3.856995291556733E-5
42-43	3.7687428037227035E-4
44-45	1.0688298990721121E-4
46-47	2.0726268621094313E-4
48-49	1.798468867256133E-4
50-51	7.62152878517116E-5
52-53	1.1571901061510003E-4
54-55	4.229158722083568E-5
56-57	0.0
58-59	1.8590379026178224E-6
60-61	0.0
62-63	7.436472101196479E-6
64-65	3.2535308827707736E-6
66-67	8.366440733723466E-6
68-69	0.0
70-71	1.8593329208992037E-6
72-73	9.761931272963694E-6
74-75	0.0
76-77	3.254334623787039E-6
78-79	0.0
80-81	1.3949407359428335E-6
82-83	6.463970352418687E-5
84-85	7.906747411735536E-6
86-87	6.51261564358561E-6
88-89	2.7684507869440013E-4
90-91	5.5850153479712394E-5
92-93	9.312502760866052E-6
94-95	6.344684310519452E-4
96-97	3.219119487123165E-5
98-99	2.7088189693288843E-5
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
34-35	19502.0
36-37	1168.0
38-39	1297.0
40-41	1157.0
42-43	1233.0
44-45	1358.0
46-47	1393.0
48-49	1448.0
50-51	1722.0
52-53	1849.0
54-55	1870.0
56-57	1946.0
58-59	2207.0
60-61	2327.0
62-63	2421.0
64-65	2706.0
66-67	3076.0
68-69	3740.0
70-71	4633.0
72-73	5258.0
74-75	6067.0
76-77	7334.0
78-79	8960.0
80-81	11832.0
82-83	14968.0
84-85	18518.0
86-87	21860.0
88-89	27440.0
90-91	45298.0
92-93	46057.0
94-95	78877.0
96-97	830957.0
98-99	9945458.0
100-101	9.6493031E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.54772197823523
#Duplication Level	Percentage of deduplicated	Percentage of total
1	48.42526881245738	10.43454229092137
2	17.60818431331243	7.588325202495582
3	9.817790145520956	6.346530374890295
4	5.875581498337884	5.0642158634659005
5	3.8802070031708746	4.180481086116366
6	2.585876965299219	3.3431854750914125
7	1.8200401574953686	2.7452403512053567
8	1.4339616245602265	2.4718885130785835
9	1.121008309659008	2.1739657852641354
>10	6.577051228432526	26.07380953180016
>50	0.5135974074206756	7.643620747553426
>100	0.3027105690645756	12.385712123628677
>500	0.0250097135720093	3.6702690721590137
>1k	0.013261396283457069	5.092419500387565
>5k	3.6473696395720323E-4	0.5247228726206573
>10k+	8.611844982322854E-5	0.26107120932130906
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.8584084545393523E-6	0.0	3.7168169090787046E-6	0.0	2.7876126818090282E-6
2	1.8584084545393523E-6	2.7876126818090282E-6	5.5752253636180564E-6	2.7876126818090282E-6	7.433633818157409E-6
3	5.5752253636180564E-6	2.7876126818090282E-6	8.362838045427085E-6	2.7876126818090282E-6	2.601771836355093E-5
4	2.2300901454472226E-5	2.7876126818090282E-6	1.7654880318123845E-5	2.7876126818090282E-6	3.530976063624769E-5
5	2.6946922590820607E-5	2.7876126818090282E-6	3.066373949989931E-5	4.646021136348381E-6	3.90265775453264E-5
6	3.809737331805672E-5	2.7876126818090282E-6	5.017702827256251E-5	5.5752253636180564E-6	4.1814190227135425E-5
7	3.9955781772596074E-5	2.7876126818090282E-6	7.061952127249538E-5	5.5752253636180564E-6	4.553100713621413E-5
8	4.460180290894445E-5	3.7168169090787046E-6	7.154872549976505E-5	5.5752253636180564E-6	4.9247824045292836E-5
9	4.553100713621413E-5	3.7168169090787046E-6	7.619474663611345E-5	5.5752253636180564E-6	5.668145786345024E-5
10-11	5.85398663179896E-5	6.504429590887733E-6	8.92036058178889E-5	5.5752253636180564E-6	7.20133276133999E-5
12-13	6.550889802251216E-5	6.504429590887733E-6	1.221903558859624E-4	5.5752253636180564E-6	9.89602502042205E-5
14-15	8.455758468154053E-5	6.969031704522571E-6	1.4588506368133915E-4	6.504429590887733E-6	1.0825229247691726E-4
16-17	1.0174786288602954E-4	8.362838045427086E-6	1.890930602493791E-4	1.1150450727236113E-5	1.1336291572690048E-4
18-19	1.1661513052234435E-4	1.0221246499966437E-5	2.025665215447894E-4	1.1150450727236113E-5	1.1336291572690048E-4
20-21	1.2126115165869274E-4	1.0221246499966437E-5	2.197567997492784E-4	1.1150450727236113E-5	1.1986734531778822E-4
22-23	1.268363770223108E-4	1.0685848613601275E-5	2.4577451811282937E-4	1.161505284087095E-5	1.235841622268669E-4
24-25	1.4123904254499079E-4	1.1150450727236113E-5	2.49491335021908E-4	1.440266552267998E-5	1.282301833632153E-4
26-27	1.6307534188582815E-4	1.4867267636314818E-5	2.5738957095370024E-4	1.8119482431758686E-5	1.3241160238592885E-4
28-29	1.821240285448565E-4	1.5796471863584494E-5	2.662170111127622E-4	1.8584084545393524E-5	1.3845142986318172E-4
30-31	2.053541342265984E-4	1.7654880318123848E-5	2.66681613226397E-4	1.95132887726632E-5	1.46349665794974E-4
32-33	2.234736166583571E-4	1.9977890886298037E-5	2.7690285972636344E-4	2.0442492999932875E-5	1.6075233131765396E-4
34-35	2.429869054310203E-4	2.0907095113567712E-5	3.233630710898473E-4	2.137169722720255E-5	1.7143817993125525E-4
36-37	2.759736554990938E-4	2.137169722720255E-5	3.4287635986251044E-4	2.137169722720255E-5	1.8862845813574425E-4
38-39	3.038497823171841E-4	2.1836299340837388E-5	3.693586803396962E-4	2.137169722720255E-5	2.113939617038513E-4
40-41	3.215046626353079E-4	2.2300901454472226E-5	4.13031279021371E-4	2.2300901454472226E-5	2.2672583145380096E-4
42-43	3.461285746579544E-4	2.648232047718577E-5	4.367259868167478E-4	2.2300901454472226E-5	2.3137185259014935E-4
44-45	3.735400993624098E-4	2.7876126818090282E-5	4.436950185212704E-4	2.2300901454472226E-5	2.3973469063557642E-4
46-47	4.0699145154411817E-4	3.0199137386264474E-5	4.664605220893774E-4	2.2300901454472226E-5	2.5227894770371703E-4
48-49	4.399782016121916E-4	3.159294372716899E-5	4.803985854984225E-4	2.2300901454472226E-5	2.7179223647638023E-4
50-51	4.734295537939E-4	3.252214795443866E-5	4.864384129756754E-4	2.32301056817419E-5	2.922347294763131E-4
52-53	5.110623249983218E-4	3.252214795443866E-5	5.226773778391928E-4	2.5088514136281256E-5	3.047789865444538E-4
54-55	5.44978279293665E-4	3.252214795443866E-5	5.62633159611789E-4	2.6946922590820607E-5	3.080312013398976E-4
56-57	5.802880399299127E-4	3.623896486351737E-5	5.946907054525928E-4	2.6946922590820607E-5	3.159294372716899E-4
58-59	6.248898428388572E-4	3.716816909078705E-5	6.090933709752726E-4	2.7876126818090282E-5	3.205754584080382E-4
60-61	6.722792584296106E-4	3.7632771204421885E-5	6.625226140432791E-4	2.880533104535996E-5	3.247568774307518E-4
62-63	7.159518571112854E-4	3.9955781772596074E-5	7.233854909294428E-4	2.9734535272629637E-5	3.280090922261957E-4
64-65	7.582306494520556E-4	4.460180290894445E-5	7.63341272702039E-4	2.9734535272629637E-5	3.321905112489092E-4
66-67	8.158413115427756E-4	4.9247824045292836E-5	7.675226917247524E-4	3.345135218170834E-5	3.3776573661252723E-4
68-69	8.613723186789898E-4	5.110623249983219E-5	7.842483678156066E-4	3.530976063624769E-5	3.438055640897802E-4
70-71	9.106201427242826E-4	5.5287651522545727E-5	7.86106776270146E-4	3.6238964863517365E-5	3.535622084761118E-4
72-73	9.631201815650192E-4	5.668145786345024E-5	8.065492692700788E-4	3.6238964863517365E-5	3.628542507488085E-4
74-75	0.001021195445769374	5.668145786345024E-5	8.139829030882363E-4	3.716816909078705E-5	3.6796487399879177E-4
76-77	0.0010769476994055547	5.7610662090719915E-5	8.195581284518544E-4	3.856197543169156E-5	3.893365712259943E-4
78-79	0.0011220141044281338	5.7610662090719915E-5	8.23274945360933E-4	3.9491179658961236E-5	3.981640113850562E-4
80-81	0.0011642928967689043	5.7610662090719915E-5	8.307085791790904E-4	3.9955781772596074E-5	4.10243666339562E-4
82-83	0.0012070362912233092	5.7610662090719915E-5	8.386068151108826E-4	4.088498599986575E-5	4.21858719180433E-4
84-85	0.00126278854485949	5.7610662090719915E-5	8.469696531563098E-4	4.27433944544051E-5	4.316153635667646E-4
86-87	0.0012892708653366755	5.85398663179896E-5	8.562616954290066E-4	4.506640502257929E-5	4.395135994985568E-4
88-89	0.0012934522843593892	5.85398663179896E-5	8.6044311445172E-4	4.831861981802316E-5	4.576330819303155E-4
90	0.0012934522843593892	5.85398663179896E-5	8.613723186789898E-4	4.831861981802316E-5	4.794693812711529E-4
>>END_MODULE
