##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	SRR21290305_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	89819288
Sequences flagged as poor quality	0
Sequence length	35-101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.36683516128518	37.0	37.0	37.0	37.0	37.0
2	36.415122440071	37.0	37.0	37.0	37.0	37.0
3	36.498076571259396	37.0	37.0	37.0	37.0	37.0
4	36.477121350594544	37.0	37.0	37.0	37.0	37.0
5	36.47582120668781	37.0	37.0	37.0	37.0	37.0
6	36.41481923125465	37.0	37.0	37.0	37.0	37.0
7	36.40226791822264	37.0	37.0	37.0	37.0	37.0
8	36.449486896400245	37.0	37.0	37.0	37.0	37.0
9	36.41756046874921	37.0	37.0	37.0	37.0	37.0
10-11	36.43201607209356	37.0	37.0	37.0	37.0	37.0
12-13	36.422121816418766	37.0	37.0	37.0	37.0	37.0
14-15	36.41424583548246	37.0	37.0	37.0	37.0	37.0
16-17	36.401517222002475	37.0	37.0	37.0	37.0	37.0
18-19	36.40655330623418	37.0	37.0	37.0	37.0	37.0
20-21	36.402919610095324	37.0	37.0	37.0	37.0	37.0
22-23	36.37791095048538	37.0	37.0	37.0	37.0	37.0
24-25	36.36716068156764	37.0	37.0	37.0	37.0	37.0
26-27	36.34615332288094	37.0	37.0	37.0	37.0	37.0
28-29	36.33502203891886	37.0	37.0	37.0	37.0	37.0
30-31	36.32219749949476	37.0	37.0	37.0	37.0	37.0
32-33	36.305132540128795	37.0	37.0	37.0	37.0	37.0
34-35	36.29246158686985	37.0	37.0	37.0	37.0	37.0
36-37	36.28036284664997	37.0	37.0	37.0	37.0	37.0
38-39	36.270058685601256	37.0	37.0	37.0	37.0	37.0
40-41	36.270496704102584	37.0	37.0	37.0	37.0	37.0
42-43	36.24315298649576	37.0	37.0	37.0	37.0	37.0
44-45	36.2361942188045	37.0	37.0	37.0	37.0	37.0
46-47	36.235980544163965	37.0	37.0	37.0	37.0	37.0
48-49	36.21830411898634	37.0	37.0	37.0	37.0	37.0
50-51	36.204586645038326	37.0	37.0	37.0	37.0	37.0
52-53	36.19503464907119	37.0	37.0	37.0	37.0	37.0
54-55	36.191142320874704	37.0	37.0	37.0	37.0	37.0
56-57	36.164256394542036	37.0	37.0	37.0	37.0	37.0
58-59	36.179837844227876	37.0	37.0	37.0	37.0	37.0
60-61	36.15386613396387	37.0	37.0	37.0	37.0	37.0
62-63	36.14542374353145	37.0	37.0	37.0	37.0	37.0
64-65	36.13602186677075	37.0	37.0	37.0	37.0	37.0
66-67	36.12053950687425	37.0	37.0	37.0	37.0	37.0
68-69	36.09548198280726	37.0	37.0	37.0	37.0	37.0
70-71	36.09757155030593	37.0	37.0	37.0	37.0	37.0
72-73	36.07972949364817	37.0	37.0	37.0	37.0	37.0
74-75	36.08838180468704	37.0	37.0	37.0	37.0	37.0
76-77	36.068861795420055	37.0	37.0	37.0	37.0	37.0
78-79	36.032516007676726	37.0	37.0	37.0	37.0	37.0
80-81	36.032210394350656	37.0	37.0	37.0	37.0	37.0
82-83	36.006808905208175	37.0	37.0	37.0	37.0	37.0
84-85	35.971746650259675	37.0	37.0	37.0	37.0	37.0
86-87	35.94118634123607	37.0	37.0	37.0	37.0	37.0
88-89	35.958823814811595	37.0	37.0	37.0	37.0	37.0
90-91	35.9108176294234	37.0	37.0	37.0	37.0	37.0
92-93	35.942044645510464	37.0	37.0	37.0	37.0	37.0
94-95	35.90241080337156	37.0	37.0	37.0	37.0	37.0
96-97	35.88155396760435	37.0	37.0	37.0	37.0	37.0
98-99	35.87796436026791	37.0	37.0	37.0	37.0	37.0
100-101	35.83953689435748	37.0	37.0	37.0	37.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	7032.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	30.0
12	367.0
13	2932.0
14	7791.0
15	11390.0
16	13950.0
17	17080.0
18	18557.0
19	21339.0
20	26981.0
21	38508.0
22	57130.0
23	80380.0
24	102855.0
25	125492.0
26	148265.0
27	188644.0
28	242224.0
29	328035.0
30	443396.0
31	639347.0
32	942282.0
33	1584882.0
34	3036314.0
35	8709375.0
36	5.4764322E7
37	1.8260388E7
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.802433610788498	15.493089052568992	18.477031796176398	45.22744554046611
2	23.400933820643523	20.917003559946764	30.483953468347813	25.1981091510619
3	25.479038101462027	21.79473868145285	22.704057815787767	30.022165401297357
4	25.063417642932677	26.121546356992585	20.51990341843659	28.295132581638143
5	26.552186384243193	30.201664596448673	22.99606784400419	20.25008117530394
6	21.262359829662557	34.97139979859462	22.209469749698325	21.5567706220445
7	18.94150450392913	20.67165827983729	39.845975128024854	20.540862088208726
8	20.83517937205426	24.16805110987962	28.361667269613505	26.635102248452615
9	21.403568795777716	22.462744950978628	30.89888979072077	25.23479646252289
10-11	23.315738735074635	30.831664406840652	23.23260417201115	22.619992686073555
12-13	22.248057850915302	25.699068172615902	27.544039517555706	24.508834458913086
14-15	22.50388056799252	26.35461966380774	26.946656457367684	24.194843310832056
16-17	23.175546398895143	26.452108155602183	26.375468742908758	23.996876702593916
18-19	22.95098595720128	26.95361524395305	26.049196250805128	24.04620254804054
20-21	23.159371233059645	26.847029066926336	26.177998552791216	23.815601147222807
22-23	22.871366590071727	26.919491713696246	26.488642455169476	23.720499241062555
24-25	22.757547163183144	26.866698183965156	26.576620678200463	23.799133974651234
26-27	22.990385204019653	26.99415662746789	26.305529347794447	23.709928820718005
28-29	22.876503961658905	26.84098662875608	26.301959572725835	23.98054983685918
30-31	22.796167314021105	26.85729920277234	26.38575340027685	23.960780082929706
32-33	23.050571474452298	26.95283888987399	26.234709863568114	23.761879772105598
34-35	23.047723907015985	26.811763597026804	26.225737043726344	23.914775452230867
36-37	22.83982796896207	26.77323960370217	26.431828032521626	23.955104394814136
38-39	23.265326811460003	26.872232801384865	26.220786339661238	23.641654047493894
40-41	23.20875007281915	26.764019475951432	26.104045651370573	23.923184799858845
42-43	22.933488849772218	26.79722816023026	26.411941461205313	23.857341528792205
44-45	23.12958163429326	26.796785493083284	26.384682941568162	23.68894993105529
46-47	23.08897117898663	26.635393568837063	26.35439081262374	23.921244439552567
48-49	22.95009813837182	26.605289855544136	26.380325538643547	24.064286467440503
50-51	23.24602831429134	26.683301997339942	26.19218725263549	23.878482435733225
52-53	23.339199667093265	26.74360633923529	26.089460465991802	23.82773352767964
54-55	22.868542791565027	26.6788701978595	26.472870947448822	23.97971606312665
56-57	23.172274465523575	26.76605553616927	26.346281585678344	23.715388412628815
58-59	23.199720337900313	26.600137590296136	26.343190964247547	23.856951107556
60-61	23.147472042890595	26.57133626155883	26.350209423591924	23.930982271958648
62-63	23.250868706498544	26.628944551870358	26.363704980619563	23.75648176101153
64-65	23.240362376622627	26.677853781393303	26.25397626202804	23.827807579956037
66-67	23.186320921841062	26.642621210792406	26.355489276914152	23.815568590452386
68-69	23.390788243949782	26.72958943007979	26.23246645465125	23.647155871319175
70-71	23.473209095332695	26.708263783201332	26.141424545025956	23.677102576440017
72-73	23.220400758952454	26.688527129636192	26.284059680254735	23.80701243115662
74-75	23.30063517059262	26.73017960399809	26.3846348056792	23.584550419730082
76-77	23.379510452312125	26.581505373373098	26.30747473718883	23.731509437125943
78-79	23.160500784321982	26.690858630324293	26.321946765115218	23.826693820238507
80-81	23.491995852007154	26.768313460018373	26.151882475058958	23.587808212915515
82-83	23.455337177499544	26.768900092839065	26.090686282295295	23.685076447366093
84-85	23.22881953377891	26.794619334557307	26.23641625987653	23.740144871787248
86-87	23.407395635636572	26.7005227570073	26.238609211133607	23.653472396222522
88-89	23.519428234812253	26.46745055025253	26.21046770066618	23.802653514269036
90-91	23.313513060901776	26.57457416371049	26.402043549104548	23.709869226283182
92-93	23.515594513356525	26.633240038017185	26.30255185584766	23.54861359277863
94-95	23.483671390045366	26.572418610988212	26.3208841388825	23.62302586008392
96-97	23.382673474601226	26.436128462746026	26.456172150857824	23.725025911794916
98-99	23.641733348708595	25.575040139594506	26.81694017409764	23.96628633759926
100-101	24.34129009033463	12.049913812123595	33.20739455185679	30.401401545684987
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	7396.0
1	3848.0
2	487.5
3	1017.5
4	1601.0
5	1985.5
6	2223.0
7	2421.5
8	2579.5
9	2653.0
10	2746.5
11	2926.0
12	3148.5
13	3371.0
14	3609.5
15	3957.5
16	4486.0
17	5169.5
18	6078.0
19	7214.5
20	8627.5
21	10685.0
22	14222.0
23	19342.5
24	25572.0
25	34183.5
26	47315.5
27	67152.0
28	91896.0
29	123222.5
30	171752.5
31	235513.5
32	327761.0
33	453650.5
34	610112.0
35	810375.0
36	1087605.0
37	1456970.5
38	1918189.0
39	2482154.0
40	3041187.5
41	3579567.5
42	4145375.5
43	4663013.5
44	5070380.0
45	5384493.5
46	5544354.0
47	5514996.0
48	5292206.5
49	4958709.5
50	4587552.5
51	4175453.0
52	3722280.0
53	3196389.0
54	2726911.0
55	2378216.5
56	2059221.0
57	1709445.0
58	1367809.5
59	1065326.0
60	874942.0
61	777622.5
62	705773.0
63	627211.5
64	523494.0
65	441105.5
66	389246.0
67	344259.5
68	281926.0
69	209717.5
70	151249.5
71	113020.5
72	84033.5
73	63670.0
74	50538.5
75	37612.0
76	24283.0
77	14877.5
78	9619.0
79	6160.5
80	4711.5
81	3698.0
82	2344.5
83	1395.0
84	1169.5
85	1060.5
86	971.0
87	898.5
88	851.5
89	779.0
90	762.0
91	804.5
92	765.5
93	744.5
94	820.5
95	826.5
96	782.0
97	713.5
98	707.0
99	961.0
100	6064.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05507614355615912
2	0.02373209638446477
3	0.00856720218044926
4	0.008383499989445474
5	0.008338966124959709
6	0.009332071302992294
7	0.007837960149494838
8	0.00847368106502915
9	0.007829053376597686
10-11	0.0078646804681863
12-13	0.007852433655452714
14-15	0.007847980269004136
16-17	0.008679650188275819
18-19	0.007903090926305272
20-21	0.007895297500020264
22-23	0.009492949888447124
24-25	0.009230756761287175
26-27	0.010204935046913308
28-29	0.01120806034445519
30-31	0.012178898590244892
32-33	0.013546644903263984
34-35	0.014320420798704173
36-37	2.165786008338688E-4
38-39	5.729100661144897E-4
40-41	6.898389067473093E-4
42-43	0.0011703461674615037
44-45	8.919684206876742E-4
46-47	6.069028934657806E-4
48-49	5.824119184240459E-4
50-51	0.0010351060590882526
52-53	3.7195398156385587E-4
54-55	2.8398167295875397E-5
56-57	1.0691262807694334E-4
58-59	1.8375958922270056E-5
60-61	5.679967731100973E-5
62-63	8.909980793422651E-6
64-65	1.8934212871329778E-5
66-67	1.1138101453580158E-5
68-69	2.4504695227698352E-5
70-71	2.840447154330043E-5
72-73	0.0
74-75	1.0583334633505276E-5
76-77	4.28936031360282E-5
78-79	6.183941024946664E-5
80-81	2.869476112224263E-4
82-83	0.0016300097534768232
84-85	1.688873193949384E-4
86-87	0.0013352711844341568
88-89	5.733091753041763E-4
90-91	0.0023303924918224115
92-93	0.0010634275708608543
94-95	0.0011310930158447028
96-97	0.0019235930596695266
98-99	0.0017237871528503444
100-101	3.439071000728795E-4
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
34-35	13304.0
36-37	1059.0
38-39	1070.0
40-41	1099.0
42-43	1090.0
44-45	1195.0
46-47	1156.0
48-49	1370.0
50-51	1507.0
52-53	1530.0
54-55	1561.0
56-57	1664.0
58-59	1873.0
60-61	2232.0
62-63	2377.0
64-65	2601.0
66-67	2976.0
68-69	3884.0
70-71	4908.0
72-73	5521.0
74-75	6440.0
76-77	7973.0
78-79	10141.0
80-81	13385.0
82-83	17399.0
84-85	21636.0
86-87	25877.0
88-89	32287.0
90-91	48429.0
92-93	51330.0
94-95	80118.0
96-97	712429.0
98-99	8320764.0
100-101	8.0417103E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.420803706765707
#Duplication Level	Percentage of deduplicated	Percentage of total
1	56.94510354703088	13.337000922364581
2	16.41872926075532	7.690796702613614
3	8.06345469894265	5.665577691070001
4	4.547583387596594	4.260322314441939
5	2.8418244024493124	3.327890574943105
6	1.9772694527663404	2.77855438371747
7	1.4179056887243549	2.324594356722369
8	1.0846018165566575	2.0321796996459986
9	0.8230440400562349	1.7348717613762439
>10	5.098327355231158	22.3411097450492
>50	0.442854640639266	7.1884271358073555
>100	0.2905184915429366	13.087005938114535
>500	0.02931665857144803	4.722809371203
>1k	0.018671957785713695	8.12686316149868
>5k	6.994730202141568E-4	1.063224445856211
>10k+	9.512833074912533E-5	0.3187717955753075
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.1133466121441532E-6
2	4.453386448576613E-6	1.1133466121441532E-6	1.1133466121441532E-6	0.0	5.566733060720766E-6
3	6.6800796728649195E-6	1.1133466121441532E-6	2.2266932242883065E-6	0.0	7.793426285009074E-6
4	2.783366530360383E-5	1.1133466121441532E-6	7.793426285009074E-6	2.2266932242883065E-6	8.906772897153226E-6
5	3.1173705140036294E-5	2.2266932242883065E-6	2.004023901859476E-5	2.2266932242883065E-6	8.906772897153226E-6
6	4.453386448576613E-5	2.2266932242883065E-6	3.34003983643246E-5	2.2266932242883065E-6	1.002011950929738E-5
7	4.564721109791028E-5	2.2266932242883065E-6	5.2327290770775203E-5	2.2266932242883065E-6	1.2246812733585687E-5
8	5.121394415863105E-5	2.2266932242883065E-6	5.3440637382919356E-5	2.2266932242883065E-6	1.7813545794306452E-5
9	5.3440637382919356E-5	2.2266932242883065E-6	5.678067721935182E-5	2.2266932242883065E-6	2.4493625467171373E-5
10-11	6.513077681043298E-5	3.896713142504537E-6	7.236752978936997E-5	2.2266932242883065E-6	3.006035852789214E-5
12-13	8.739770905331602E-5	6.6800796728649195E-6	9.018107558367641E-5	2.2266932242883065E-6	4.5090537791838206E-5
14-15	1.0521125484762249E-4	7.793426285009074E-6	1.0632460145976663E-4	2.2266932242883065E-6	4.843057762827066E-5
16-17	1.1801474088728025E-4	9.463446203225302E-6	1.3527161337551462E-4	2.2266932242883065E-6	5.01005975464869E-5
18-19	1.263648404783614E-4	1.1133466121441532E-5	1.3972499982409123E-4	2.2266932242883065E-6	5.0657270852558975E-5
20-21	1.336015934572984E-4	1.1133466121441532E-5	1.4918844602731653E-4	2.2266932242883065E-6	5.121394415863105E-5
22-23	1.480750994151724E-4	1.1133466121441532E-5	1.67001991821623E-4	2.2266932242883065E-6	5.566733060720766E-5
24-25	1.7423874480056E-4	1.6143525876090223E-5	1.6978535835198338E-4	2.783366530360383E-6	5.678067721935182E-5
26-27	2.070824698588125E-4	1.8926892406450604E-5	1.8203217108556905E-4	3.3400398364324597E-6	5.900737044364013E-5
28-29	2.6330647377209224E-4	2.171025893681099E-5	1.8926892406450604E-4	3.3400398364324597E-6	6.847081664686542E-5
30-31	3.095103581760746E-4	2.338027885502722E-5	1.9260896390093851E-4	3.3400398364324597E-6	7.960428276830696E-5
32-33	3.4513744976468754E-4	2.4493625467171373E-5	1.998457168798755E-4	3.3400398364324597E-6	9.018107558367641E-5
34-35	3.9134133416866986E-4	2.4493625467171373E-5	2.265660355713352E-4	3.3400398364324597E-6	9.630448195046927E-5
36-37	4.442252982455172E-4	2.4493625467171373E-5	2.3268944193812803E-4	3.3400398364324597E-6	1.0354123492940625E-4
38-39	5.076860551377338E-4	2.4493625467171373E-5	2.4827629450814613E-4	3.896713142504537E-6	1.169013942751361E-4
40-41	5.644667323570857E-4	2.4493625467171373E-5	2.755532865056779E-4	4.453386448576613E-6	1.2747818709050557E-4
42-43	6.401743019828881E-4	2.5606972079315526E-5	2.816766928724708E-4	7.236752978936996E-6	1.3248824684515423E-4
44-45	7.169952182208346E-4	2.8947011915747986E-5	2.861300793210474E-4	8.906772897153226E-6	1.3694163329373084E-4
46-47	7.954861543769975E-4	2.8947011915747986E-5	2.9893356536070517E-4	8.906772897153226E-6	1.386116532119471E-4
48-49	8.812138435120972E-4	3.006035852789214E-5	3.2342719082787655E-4	8.906772897153226E-6	1.4139501974230748E-4
50-51	9.847550784415036E-4	3.3957071670396675E-5	3.295505971946694E-4	8.906772897153226E-6	1.4250836635445162E-4
52-53	0.001082172907004117	3.5627091588612904E-5	3.406840633161109E-4	8.906772897153226E-6	1.4473505957873992E-4
54-55	0.0011712406359756494	3.618376489468498E-5	3.551575692739849E-4	8.906772897153226E-6	1.4918844602731653E-4
56-57	0.0012619783848653978	4.008047803718952E-5	3.7241444176221926E-4	8.906772897153226E-6	1.692286850459113E-4
58-59	0.0013638495998765877	4.119382464933367E-5	3.835479078836608E-4	8.906772897153226E-6	1.8203217108556905E-4
60-61	0.0014696175280302821	4.6760557710054435E-5	4.241850592269224E-4	8.906772897153226E-6	1.837021910037853E-4
62-63	0.0015753854561839768	4.6760557710054435E-5	4.698322703248327E-4	1.2246812733585687E-5	1.837021910037853E-4
64-65	0.0016850500974801758	4.6760557710054435E-5	4.909858559555716E-4	1.5030179263946071E-5	1.8759890414628982E-4
66-67	0.0018125282845706816	5.01005975464869E-5	4.976659356284365E-4	1.5586852570018147E-5	1.88712250758434E-4
68-69	0.0019060493999907904	5.288396407684728E-5	5.021193220770132E-4	1.6143525876090223E-5	1.9594900373737099E-4
70-71	0.001998457168798755	5.399731068899143E-5	5.032326686891574E-4	1.67001991821623E-5	2.0429910332845212E-4
72-73	0.0021264920291953326	5.5110657301135584E-5	5.177061746470313E-4	1.67001991821623E-5	2.076391431648846E-4
74-75	0.0022567535828161987	5.789402383149597E-5	5.2271623440168E-4	1.67001991821623E-5	2.148758961438216E-4
76-77	0.0023619648376638214	6.290408358614467E-5	5.266129475441845E-4	1.67001991821623E-5	2.215559758166865E-4
78-79	0.002449362546717137	7.014083656508166E-5	5.321796806049053E-4	1.67001991821623E-5	2.2767938218347936E-4
80-81	0.0025367602557704533	7.181085648329789E-5	5.366330670534819E-4	1.7256872488234376E-5	2.3213276863205597E-4
82-83	0.00260022101266267	7.459422301365827E-5	5.37746413665626E-4	1.7813545794306452E-5	2.3881284830492086E-4
84-85	0.0026853920284916977	8.238764929866735E-5	5.399731068899144E-4	1.7813545794306452E-5	2.4159621483528125E-4
86-87	0.002732152586201752	8.628436244117189E-5	5.416431268081306E-4	1.7813545794306452E-5	2.47162947896002E-4
88-89	0.0027421727057110497	8.684103574724395E-5	5.433131467263468E-4	1.8926892406450604E-5	2.6052310724173185E-4
90	0.0027421727057110497	9.129442219582056E-5	5.499932263992117E-4	1.8926892406450604E-5	2.627498004660202E-4
>>END_MODULE
