##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	SRR21290303_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	89049905
Sequences flagged as poor quality	0
Sequence length	35-101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.123483343412886	37.0	37.0	37.0	37.0	37.0
2	36.10543838311787	37.0	37.0	37.0	37.0	37.0
3	36.19944269452056	37.0	37.0	37.0	37.0	37.0
4	36.17365239188071	37.0	37.0	37.0	37.0	37.0
5	36.15124414787416	37.0	37.0	37.0	37.0	37.0
6	36.061257145642095	37.0	37.0	37.0	37.0	37.0
7	36.014283148308806	37.0	37.0	37.0	37.0	37.0
8	36.103255550918334	37.0	37.0	37.0	37.0	37.0
9	36.05299485720956	37.0	37.0	37.0	37.0	37.0
10-11	36.08223135667579	37.0	37.0	37.0	37.0	37.0
12-13	36.06451410026771	37.0	37.0	37.0	37.0	37.0
14-15	36.0551649830508	37.0	37.0	37.0	37.0	37.0
16-17	36.041895120494516	37.0	37.0	37.0	37.0	37.0
18-19	36.05231211083269	37.0	37.0	37.0	37.0	37.0
20-21	36.04214704103278	37.0	37.0	37.0	37.0	37.0
22-23	36.01572541262116	37.0	37.0	37.0	37.0	37.0
24-25	35.99875141921825	37.0	37.0	37.0	37.0	37.0
26-27	35.96685039697684	37.0	37.0	37.0	37.0	37.0
28-29	35.950401536082495	37.0	37.0	37.0	37.0	37.0
30-31	35.93245655343485	37.0	37.0	37.0	37.0	37.0
32-33	35.913202821496554	37.0	37.0	37.0	37.0	37.0
34-35	35.89561406045296	37.0	37.0	37.0	37.0	37.0
36-37	35.88203283779585	37.0	37.0	37.0	37.0	37.0
38-39	35.873782412515425	37.0	37.0	37.0	37.0	37.0
40-41	35.87815972530321	37.0	37.0	37.0	37.0	37.0
42-43	35.83954862552309	37.0	37.0	37.0	37.0	37.0
44-45	35.82512153578119	37.0	37.0	37.0	37.0	37.0
46-47	35.83043995327968	37.0	37.0	37.0	37.0	37.0
48-49	35.80778811254936	37.0	37.0	37.0	37.0	37.0
50-51	35.7910553088352	37.0	37.0	37.0	37.0	37.0
52-53	35.779261466258845	37.0	37.0	37.0	37.0	37.0
54-55	35.775714113381554	37.0	37.0	37.0	37.0	37.0
56-57	35.7427137808798	37.0	37.0	37.0	37.0	37.0
58-59	35.76459858976827	37.0	37.0	37.0	37.0	37.0
60-61	35.73280717012216	37.0	37.0	37.0	37.0	37.0
62-63	35.722439533167346	37.0	37.0	37.0	37.0	37.0
64-65	35.708177912370914	37.0	37.0	37.0	37.0	37.0
66-67	35.68939034492619	37.0	37.0	37.0	37.0	37.0
68-69	35.65402088253157	37.0	37.0	37.0	37.0	37.0
70-71	35.66449934206642	37.0	37.0	37.0	37.0	37.0
72-73	35.6435588552235	37.0	37.0	37.0	37.0	37.0
74-75	35.65505925431953	37.0	37.0	37.0	37.0	37.0
76-77	35.63133056481651	37.0	37.0	37.0	37.0	37.0
78-79	35.58392489318635	37.0	37.0	37.0	37.0	37.0
80-81	35.58208960021162	37.0	37.0	37.0	37.0	37.0
82-83	35.55024010834168	37.0	37.0	37.0	37.0	37.0
84-85	35.50981964346667	37.0	37.0	37.0	37.0	37.0
86-87	35.4732222986604	37.0	37.0	37.0	37.0	37.0
88-89	35.50110478232054	37.0	37.0	37.0	37.0	37.0
90-91	35.443107874756706	37.0	37.0	37.0	37.0	37.0
92-93	35.48940032521533	37.0	37.0	37.0	37.0	37.0
94-95	35.44490791603569	37.0	37.0	37.0	37.0	37.0
96-97	35.42297310985305	37.0	37.0	37.0	37.0	37.0
98-99	35.41937501137118	37.0	37.0	37.0	37.0	37.0
100-101	35.39770253440564	37.0	37.0	37.0	37.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	16581.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	36.0
12	1774.0
13	12293.0
14	24096.0
15	25971.0
16	26270.0
17	30713.0
18	35882.0
19	40250.0
20	44677.0
21	59824.0
22	83856.0
23	118835.0
24	157800.0
25	200891.0
26	248525.0
27	324065.0
28	420458.0
29	568129.0
30	760412.0
31	1086921.0
32	1593789.0
33	2671440.0
34	5011800.0
35	1.3308083E7
36	5.2455082E7
37	9721452.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.50279603627515	16.068508366758604	13.002656978909913	43.42603861805633
2	22.83231663143107	19.824005428421355	30.918576353273004	26.42510158687457
3	24.451893275290185	20.129291945429213	23.15345449529928	32.26536028398132
4	25.488234540330446	25.076058228580617	20.674804771047622	28.760902460041315
5	26.239051265355904	28.96586725583096	23.009652950620644	21.785428528192487
6	21.784910365194694	33.690208807982486	22.965443353175	21.55943747364781
7	19.097679334977023	21.974903439235078	38.119812791425325	20.807604434362574
8	20.323297065284834	24.296651627964813	29.094613060776787	26.28543824597357
9	21.12301752733676	22.410178319642824	31.351604867126092	25.115199285894324
10-11	22.965543524843955	30.607818292066007	23.999990812425796	22.42664737066424
12-13	22.314077545484004	25.849880104141025	27.602935815869404	24.233106534505566
14-15	22.329238283704203	26.440579754909155	27.120638693522718	24.109543267863927
16-17	23.115821259343768	26.44438530626385	26.554900522237734	23.884892912154648
18-19	22.888940713460592	26.90334451995673	26.05197907449665	24.15573569208603
20-21	22.875800087102267	26.736507221597765	26.306324798228527	24.08136789307144
22-23	22.746740104728794	26.854487625932773	26.433210701378158	23.965561567960275
24-25	22.75757021779151	26.609631691351833	26.55686182574356	24.07593626511309
26-27	22.9431601806323	26.82253314048138	26.357242285932088	23.87706439295423
28-29	22.786943250553165	26.637147028031205	26.414741905028123	24.16116781638751
30-31	22.803825770651354	26.365013929002114	26.575672468287557	24.25548783205897
32-33	22.96762130340996	26.601407236393975	26.402016911488335	24.02895454870773
34-35	22.91356647971122	26.66272025679863	26.308217347798017	24.11549591569213
36-37	22.648249294848263	26.594673792459417	26.542579558887976	24.214497353804347
38-39	23.01287484830648	26.72716617604899	26.455087557818104	23.804871417826426
40-41	23.14741338647365	26.58083866873408	26.078393490201762	24.193354454590505
42-43	22.93143557249971	26.702908383112835	26.27128658297464	24.094369461412818
44-45	23.083682013421118	26.590901118649356	26.422732012296322	23.9026848556332
46-47	23.002159898185255	26.321767676065544	26.478807061031695	24.197265364717506
48-49	22.77536479625173	26.164812195471367	26.641616274013803	24.418206734263105
50-51	23.027286356801376	26.168380286386906	26.470968905583565	24.333364451228153
52-53	23.232312525288314	26.475411848647134	26.174959869416075	24.117315756648473
54-55	22.79475842218782	26.44831711847422	26.503296370508206	24.25362808882976
56-57	22.920992459610666	26.636593103321744	26.367213754570397	24.07520068249719
58-59	22.96220374322007	26.536448535831948	26.314820654222455	24.186527066725528
60-61	22.99844862993209	26.37226140206439	26.326308489221727	24.302981478781792
62-63	23.112848889096306	26.237070043080912	26.335964579001057	24.31411648882173
64-65	23.158030619040673	26.425166038900905	26.129905056629372	24.28689828542905
66-67	23.190871895386287	26.336433016757127	26.227424582418855	24.245270505437734
68-69	23.121176139780264	26.43660460205672	26.4510243647131	23.991194893449922
70-71	23.464633499644773	26.418953400941742	26.226531284289578	23.88988181512391
72-73	23.18569481399159	26.35076232610688	26.21011693213284	24.253425927768685
74-75	23.284086901409207	26.523448879554874	26.206645092174917	23.985819126861006
76-77	23.25938862222138	26.503047664427143	26.189811975197653	24.047751738153824
78-79	23.07838092543025	26.557222651676454	26.047405948258273	24.316990474635023
80-81	23.34859016284292	26.47653983376612	26.066142357401883	24.108727645989074
82-83	23.390998684193	26.54964191424994	25.84531846570149	24.214040935855575
84-85	23.265370557132805	26.59758832404587	25.96790102438739	24.16914009443393
86-87	23.269034802041297	26.53402338773749	26.028371120267135	24.168570689954077
88-89	23.43393801498488	26.296230707875047	25.93400256980456	24.335828707335516
90-91	23.29461561301781	26.304117092133133	26.240567071340752	24.1607002235083
92-93	23.652731257820882	26.191869467679147	26.122293501405615	24.033105773094363
94-95	23.458959862985594	26.266125713384763	26.173896605042835	24.101017818586808
96-97	23.43929293953778	26.047590297986716	26.270434917532004	24.242681844943498
98-99	23.64778022799352	25.249788222934992	26.664153839634718	24.438277709436772
100-101	24.611588726927085	11.75390557301086	32.61413222726047	31.020373472801587
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	16720.0
1	8442.5
2	279.0
3	571.5
4	962.0
5	1373.5
6	1750.5
7	2024.0
8	2307.5
9	2600.0
10	2819.5
11	3025.5
12	3251.5
13	3527.0
14	3829.5
15	4209.0
16	4711.0
17	5391.0
18	6151.0
19	7090.0
20	8422.0
21	10659.0
22	14743.0
23	20756.5
24	27853.0
25	36716.5
26	46557.5
27	63757.5
28	86640.5
29	112399.5
30	154604.5
31	206941.5
32	295128.5
33	424133.0
34	566802.5
35	735200.0
36	998204.0
37	1361987.0
38	1790074.5
39	2378230.0
40	2958091.5
41	3451686.0
42	3979140.0
43	4535475.0
44	4984626.0
45	5281619.5
46	5416406.0
47	5382696.0
48	5214114.5
49	4984361.0
50	4680780.0
51	4312311.0
52	3817442.5
53	3168314.0
54	2644759.5
55	2294878.0
56	2012817.0
57	1710050.0
58	1426481.5
59	1143547.0
60	933294.5
61	829297.5
62	778261.5
63	718527.5
64	589651.5
65	471913.0
66	414728.5
67	371588.0
68	300742.5
69	226776.5
70	163345.5
71	116943.5
72	91426.0
73	72478.0
74	61209.5
75	46765.5
76	30003.0
77	23907.5
78	19800.0
79	16757.5
80	14906.0
81	11620.5
82	8296.5
83	6318.5
84	6173.0
85	6091.5
86	5991.5
87	5847.0
88	5613.0
89	5205.0
90	4814.0
91	4403.5
92	3844.5
93	3340.5
94	2846.5
95	2433.0
96	2064.0
97	1795.5
98	1689.0
99	1940.5
100	9055.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.06496918778296282
2	0.03483776877695715
3	0.01941607910755211
4	0.019238650507263314
5	0.019234158643964865
6	0.020119055713759607
7	0.018630003030323278
8	0.01928806100354627
9	0.0186232652353756
10-11	0.018653023829727836
12-13	0.01863730230818326
14-15	0.01863281044488481
16-17	0.019504231924784197
18-19	0.01867997500951854
20-21	0.01867492166330778
22-23	0.02057891021893847
24-25	0.021312768385322815
26-27	0.02318475241495204
28-29	0.02501911708945675
30-31	0.02650929273871769
32-33	0.028382399734171528
34-35	0.029619346589982328
36-37	1.870313779285147E-4
38-39	5.285255073514893E-4
40-41	6.251423771593263E-4
42-43	0.0010351801683340005
44-45	8.43099251722172E-4
46-47	6.324764253259266E-4
48-49	5.903598792819087E-4
50-51	0.0010223356924965227
52-53	3.892817620045227E-4
54-55	3.2581285546638945E-5
56-57	9.212845234171286E-5
58-59	1.7414916336494835E-5
60-61	4.438101791504955E-5
62-63	2.808993056438828E-6
64-65	2.0787127504998448E-5
66-67	1.0112932522047668E-5
68-69	1.7417204928529623E-5
70-71	2.2474488239187393E-5
72-73	5.618803460632289E-7
74-75	8.428486260724546E-6
76-77	3.090554766212259E-5
78-79	5.11366275889737E-5
80-81	2.8098213393646184E-4
82-83	0.0016466283794659036
84-85	1.6972886768814221E-4
86-87	0.0012674124484969669
88-89	5.306001430484496E-4
90-91	0.002349089784201449
92-93	9.484577368806651E-4
94-95	0.0011650519807171302
96-97	0.0018024857404210337
98-99	0.0017704337393694145
100-101	3.455517902728697E-4
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
34-35	27109.0
36-37	1228.0
38-39	1503.0
40-41	1372.0
42-43	1500.0
44-45	1640.0
46-47	1648.0
48-49	1614.0
50-51	1716.0
52-53	1931.0
54-55	2018.0
56-57	1959.0
58-59	2110.0
60-61	2106.0
62-63	2420.0
64-65	2443.0
66-67	2505.0
68-69	2560.0
70-71	2869.0
72-73	2921.0
74-75	3146.0
76-77	3384.0
78-79	3576.0
80-81	3969.0
82-83	4075.0
84-85	4642.0
86-87	4758.0
88-89	5525.0
90-91	14673.0
92-93	10603.0
94-95	30923.0
96-97	640976.0
98-99	8075235.0
100-101	8.0179248E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.460077273818364
#Duplication Level	Percentage of deduplicated	Percentage of total
1	61.839591265927375	13.270824071481444
2	15.01254538566167	6.443407681060094
3	7.203396033278543	4.637563065242226
4	3.993187103073928	3.4277641520312554
5	2.5460732333955303	2.7319464166734324
6	1.6811641129557298	2.164674706440016
7	1.1861046520730942	1.7817728241826833
8	0.8650371090980873	1.4851010564772318
9	0.7006182474287646	1.3531789556341631
>10	4.209313461039169	16.974322798783888
>50	0.39317032160460874	5.867379878458747
>100	0.29502135383394784	12.623784727431039
>500	0.03797539162901602	5.6450766144183175
>1k	0.032885773941712396	14.287249341865577
>5k	0.0030403287768550626	4.374141762705476
>10k+	8.762262819176098E-4	2.9318119471147086
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.1229658246126147E-6	0.0	2.2459316492252293E-6	2.2459316492252293E-6	1.1229658246126147E-6
2	1.1229658246126147E-6	0.0	4.491863298450459E-6	2.2459316492252293E-6	4.491863298450459E-6
3	7.860760772288304E-6	0.0	4.491863298450459E-6	2.2459316492252293E-6	7.860760772288304E-6
4	1.7967453193801835E-5	0.0	8.983726596900917E-6	3.368897473837844E-6	7.860760772288304E-6
5	2.1336350667639678E-5	0.0	1.1229658246126147E-5	3.368897473837844E-6	1.2352624070738762E-5
6	3.593490638760367E-5	0.0	1.684448736918922E-5	3.368897473837844E-6	1.4598555719963992E-5
7	3.593490638760367E-5	0.0	2.1336350667639678E-5	3.368897473837844E-6	1.4598555719963992E-5
8	4.2672701335279356E-5	0.0	2.358228231686491E-5	3.368897473837844E-6	1.4598555719963992E-5
9	4.379566715989197E-5	1.1229658246126147E-6	2.5828213966090137E-5	3.368897473837844E-6	1.4598555719963992E-5
10-11	6.344756909061273E-5	1.1229658246126147E-6	4.0988252598360436E-5	3.368897473837844E-6	2.5266731053783832E-5
12-13	7.467722733673887E-5	1.1229658246126147E-6	5.558680831832443E-5	5.614829123063073E-6	2.9197111439927983E-5
14-15	9.545209509207224E-5	1.1229658246126147E-6	6.457053491522534E-5	5.614829123063073E-6	3.593490638760367E-5
16-17	1.1398103119818038E-4	1.1229658246126147E-6	8.534540267055871E-5	8.42224368459461E-6	4.323418424758567E-5
18-19	1.2689513818122544E-4	1.1229658246126147E-6	1.0218989003974794E-4	8.983726596900917E-6	4.828753045834243E-5
20-21	1.386862793396579E-4	1.1229658246126147E-6	1.1061213372434256E-4	1.0106692421513533E-5	4.997197919526136E-5
22-23	1.5777669835807237E-4	1.1229658246126147E-6	1.1734992867201824E-4	1.0106692421513533E-5	5.0533462107567664E-5
24-25	1.774286002887931E-4	2.2459316492252293E-6	1.1903437740893715E-4	1.0106692421513533E-5	5.0533462107567664E-5
26-27	2.1560943832562202E-4	3.368897473837844E-6	1.2521068944430655E-4	1.0106692421513533E-5	5.3902359581405504E-5
28-29	2.526673105378383E-4	4.491863298450459E-6	1.308255185673696E-4	1.0106692421513533E-5	5.502532540601812E-5
30-31	2.9477852896081134E-4	5.0533462107567664E-6	1.3250996730428854E-4	1.0106692421513533E-5	5.783273996754966E-5
32-33	3.3071343534841504E-4	6.17631203536938E-6	1.3363293312890114E-4	1.1229658246126147E-5	5.951718870446858E-5
34-35	3.660868588237124E-4	6.737794947675688E-6	1.6283004456882913E-4	1.2914106983045068E-5	5.951718870446858E-5
36-37	4.2055070131742417E-4	6.737794947675688E-6	1.7012932242881112E-4	1.3475589895351376E-5	6.232460326600011E-5
38-39	4.907360653557127E-4	7.860760772288304E-6	1.7799008320109943E-4	1.3475589895351376E-5	6.737794947675688E-5
40-41	5.379006299894425E-4	8.42224368459461E-6	1.8978122435953188E-4	1.3475589895351376E-5	7.130832986290103E-5
42-43	6.035941307292804E-4	1.1229658246126147E-5	2.0269533134257696E-4	1.5721521544576604E-5	7.69231589859641E-5
44-45	6.546890757491543E-4	1.2914106983045068E-5	2.0381829716718957E-4	1.7967453193801835E-5	7.860760772288302E-5
46-47	7.181366448397671E-4	1.3475589895351376E-5	2.1055609211486525E-4	2.0213384843027066E-5	8.309947102133348E-5
48-49	7.984287012995691E-4	1.5160038632270298E-5	2.217857503609914E-4	2.0213384843027066E-5	8.42224368459461E-5
50-51	8.764748261101457E-4	1.7967453193801835E-5	2.2459316492252294E-4	2.0213384843027066E-5	8.759133431978394E-5
52-53	9.623817116930107E-4	1.7967453193801835E-5	2.3469985734403647E-4	2.0213384843027066E-5	8.815281723209025E-5
54-55	0.0010415508023282002	1.7967453193801835E-5	2.4480654976555E-4	2.1897833579945986E-5	9.039874888131549E-5
56-57	0.001127457687911065	1.7967453193801835E-5	2.5603620801167615E-4	2.30207994045586E-5	9.938247547821639E-5
58-59	0.001229086095038507	1.7967453193801835E-5	2.6501993460857707E-4	2.358228231686491E-5	1.1117361663664886E-4
60-61	0.0013250996730428855	1.7967453193801835E-5	2.891636998377483E-4	2.5828213966090137E-5	1.1285806537356777E-4
62-63	0.0014418881188025972	1.7967453193801835E-5	3.149919138038384E-4	2.6389696878396444E-5	1.1622696284740563E-4
64-65	0.0015569921158253902	1.8528936106108142E-5	3.335208499099466E-4	2.6951179790702752E-5	1.235262407073876E-4
66-67	0.0016749035274097148	1.909041901841445E-5	3.3632826447147813E-4	2.8074145615315368E-5	1.3419441604120748E-4
68-69	0.001781023797835607	1.909041901841445E-5	3.475579227176042E-4	2.9197111439927983E-5	1.4093221098888313E-4
70-71	0.0018865825853491927	1.909041901841445E-5	3.4980385436682944E-4	3.1443043089153214E-5	1.471085230242525E-4
72-73	0.002005055479845823	1.909041901841445E-5	3.582260980514241E-4	3.1443043089153214E-5	1.5496928379654082E-4
74-75	0.00213026616929013	1.909041901841445E-5	3.604720297006493E-4	3.1443043089153214E-5	1.5721521544576606E-4
76-77	0.0022448086834006165	2.2459316492252293E-5	3.6496389299909974E-4	3.256600891376583E-5	1.5721521544576606E-4
78-79	0.0023178014620004366	2.358228231686491E-5	3.7057872212216284E-4	3.425045765068474E-5	1.678833907795859E-4
80-81	0.0023975320355479326	2.5266731053783832E-5	3.750705854206133E-4	3.4811940562991054E-5	1.757441515518742E-4
82-83	0.0024637870192000767	2.5828213966090137E-5	3.778779999821448E-4	3.4811940562991054E-5	1.7799008320109943E-4
84-85	0.0025429561098352657	2.8074145615315368E-5	3.790009658067575E-4	3.4811940562991054E-5	1.8921974144722558E-4
86-87	0.0025788910162228697	2.8635628527621675E-5	3.874232094913521E-4	3.4811940562991054E-5	1.8978122435953188E-4
88-89	0.0025839443624336264	2.9197111439927983E-5	3.874232094913521E-4	3.4811940562991054E-5	1.9595753639490126E-4
90	0.0025839443624336264	3.0320077264540595E-5	3.919150727898025E-4	3.4811940562991054E-5	1.9651901930720756E-4
>>END_MODULE
