##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	SRR21290295_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	88251117
Sequences flagged as poor quality	0
Sequence length	35-101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.00329504044691	37.0	37.0	37.0	37.0	37.0
2	35.947751550838724	37.0	37.0	37.0	37.0	37.0
3	36.05191754116834	37.0	37.0	37.0	37.0	37.0
4	36.03046217533994	37.0	37.0	37.0	37.0	37.0
5	36.00149688756914	37.0	37.0	37.0	37.0	37.0
6	35.91215938943866	37.0	37.0	37.0	37.0	37.0
7	35.86118721874081	37.0	37.0	37.0	37.0	37.0
8	35.939708899095294	37.0	37.0	37.0	37.0	37.0
9	35.87910874827794	37.0	37.0	37.0	37.0	37.0
10-11	35.920882072234846	37.0	37.0	37.0	37.0	37.0
12-13	35.899372491795205	37.0	37.0	37.0	37.0	37.0
14-15	35.891375782813036	37.0	37.0	37.0	37.0	37.0
16-17	35.87031845727233	37.0	37.0	37.0	37.0	37.0
18-19	35.876871280847354	37.0	37.0	37.0	37.0	37.0
20-21	35.868288703926545	37.0	37.0	37.0	37.0	37.0
22-23	35.833996905670894	37.0	37.0	37.0	37.0	37.0
24-25	35.82208788360152	37.0	37.0	37.0	37.0	37.0
26-27	35.791188308698686	37.0	37.0	37.0	37.0	37.0
28-29	35.771102795220145	37.0	37.0	37.0	37.0	37.0
30-31	35.74930952432024	37.0	37.0	37.0	37.0	37.0
32-33	35.7232666584832	37.0	37.0	37.0	37.0	37.0
34-35	35.704362818433225	37.0	37.0	37.0	37.0	37.0
36-37	35.69115571566258	37.0	37.0	37.0	37.0	37.0
38-39	35.6760378361797	37.0	37.0	37.0	37.0	37.0
40-41	35.67887678749524	37.0	37.0	37.0	37.0	37.0
42-43	35.63646168655443	37.0	37.0	37.0	37.0	37.0
44-45	35.62182377907271	37.0	37.0	37.0	37.0	37.0
46-47	35.626199470035246	37.0	37.0	37.0	37.0	37.0
48-49	35.595670472862295	37.0	37.0	37.0	37.0	37.0
50-51	35.573212481416036	37.0	37.0	37.0	37.0	37.0
52-53	35.55996482201689	37.0	37.0	37.0	37.0	37.0
54-55	35.55384997352084	37.0	37.0	37.0	37.0	37.0
56-57	35.517161330319254	37.0	37.0	37.0	37.0	37.0
58-59	35.5403434901184	37.0	37.0	37.0	37.0	37.0
60-61	35.5013947504785	37.0	37.0	37.0	37.0	37.0
62-63	35.48693381663784	37.0	37.0	37.0	37.0	37.0
64-65	35.474380433708866	37.0	37.0	37.0	37.0	37.0
66-67	35.45463620346028	37.0	37.0	37.0	37.0	37.0
68-69	35.41427415143164	37.0	37.0	37.0	37.0	37.0
70-71	35.418823434868244	37.0	37.0	37.0	37.0	37.0
72-73	35.39460998726713	37.0	37.0	37.0	37.0	37.0
74-75	35.40704203444865	37.0	37.0	37.0	37.0	37.0
76-77	35.380373775261205	37.0	37.0	37.0	37.0	37.0
78-79	35.33054176943609	37.0	37.0	37.0	37.0	37.0
80-81	35.3247528750923	37.0	37.0	37.0	37.0	37.0
82-83	35.28657749927302	37.0	37.0	37.0	37.0	37.0
84-85	35.23732504307873	37.0	37.0	37.0	31.0	37.0
86-87	35.192607554319835	37.0	37.0	37.0	25.0	37.0
88-89	35.21812472607539	37.0	37.0	37.0	31.0	37.0
90-91	35.14954686717121	37.0	37.0	37.0	25.0	37.0
92-93	35.19471604870522	37.0	37.0	37.0	25.0	37.0
94-95	35.1401363919626	37.0	37.0	37.0	25.0	37.0
96-97	35.11078444800359	37.0	37.0	37.0	25.0	37.0
98-99	35.1044309781758	37.0	37.0	37.0	25.0	37.0
100-101	35.081566288810436	37.0	37.0	37.0	25.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	23481.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	49.0
12	2386.0
13	15245.0
14	27658.0
15	27372.0
16	23631.0
17	24077.0
18	27882.0
19	36401.0
20	52279.0
21	82090.0
22	122051.0
23	175141.0
24	226155.0
25	279173.0
26	334166.0
27	427523.0
28	544546.0
29	722716.0
30	959431.0
31	1368599.0
32	1990516.0
33	3287097.0
34	6014519.0
35	1.4877903E7
36	4.8766417E7
37	7812613.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.175994603174246	16.98970137805321	11.336886773557445	46.497417245215104
2	21.913793455647664	19.40806472540717	33.08492455030281	25.59321726864236
3	23.21943166898132	19.82930431475628	23.446161404273784	33.50510261198862
4	24.30879573234263	24.823591978880785	21.313664041627824	29.55394824714876
5	25.84468215644584	28.792867869220846	23.39810360091858	21.96434637341473
6	21.77583986122987	33.327727684477495	23.32855510046417	21.567877353828464
7	19.03568341629242	22.900265600491128	37.598525124041934	20.465525859174512
8	19.46495619818759	24.594187699131798	30.227002811620473	25.713853291060136
9	20.472163620024908	22.737148935963784	32.16303789438493	24.62764954962638
10-11	22.39409914381274	30.824090104634884	24.49630517279096	22.285505578761413
12-13	21.919887942571453	26.342704843283883	27.50896947030804	24.22843774383663
14-15	21.99507724460191	26.776643865436178	27.044994066852407	24.183284823109506
16-17	22.808542081392655	26.74362525690569	26.38885545238524	24.058977209316414
18-19	22.622048367798993	27.03333378179475	26.06182071201021	24.28279713839605
20-21	22.755779784723586	26.887537588296922	26.32451702128475	24.032165605694743
22-23	22.54591261011264	26.970346163567505	26.458431468201603	24.02530975811825
24-25	22.42151850578535	26.872272473945312	26.577895496881567	24.12831352338777
26-27	22.537512367458802	27.021830000095896	26.495345156355306	23.945312476089995
28-29	22.452609586443653	26.93906945613961	26.42494300125976	24.183377956156978
30-31	22.448383507286803	26.734755709192136	26.503428554667924	24.313432228853138
32-33	22.70497256989413	26.85197276451718	26.34946417786691	24.09359048772178
34-35	22.730392615289656	26.86879739909122	26.193103696346125	24.207706289272995
36-37	22.461746803870312	26.77712930964406	26.460183418401968	24.30094046808366
38-39	22.71737630204462	26.93479913359493	26.47151127636066	23.876313287999796
40-41	22.839219173071605	26.82411284140227	26.20240772969324	24.13426025583289
42-43	22.69477462824009	26.739905713486444	26.415447334195274	24.149872324078185
44-45	22.765656391969202	26.759103339081	26.4754445551111	23.999795713838694
46-47	22.729609679608025	26.73622795335942	26.34232447154102	24.191837895491535
48-49	22.617196800215815	26.560010103951466	26.459373672475913	24.363419423356813
50-51	22.845589921574053	26.571200172813263	26.35890897214177	24.224300933470914
52-53	23.07628376929501	26.708665512972907	26.097658815517466	24.117391902214617
54-55	22.75110150075614	26.636364048190536	26.35144256084745	24.261091890205876
56-57	22.814480604095817	26.793889125360877	26.353630522376903	24.037999748166406
58-59	22.836482424724196	26.671893667518464	26.288929358370023	24.202694549387317
60-61	22.8335161032332	26.531987915373183	26.298037642738525	24.336458338655092
62-63	22.921561382641585	26.50612996264774	26.3667093763524	24.205599278358278
64-65	23.01019615404853	26.6619873091419	26.10252057285212	24.22529596395745
66-67	23.006635124691336	26.59241053173613	26.177795764087875	24.22315857948466
68-69	23.042185956783207	26.70954284866906	26.25110742922186	23.997163765325872
70-71	23.28682220977083	26.648124442060105	26.080419384841203	23.984633963327862
72-73	23.05979709265522	26.569043972634855	26.153413115875097	24.21774581883483
74-75	23.11008484259674	26.64267537650839	26.18356277742792	24.063677003466946
76-77	23.174756430322415	26.606108877332428	26.09797711866021	24.121157573684947
78-79	22.971232531594758	26.63905253524492	26.151992675347017	24.237722257813303
80-81	23.14966502142478	26.666053169207505	26.117868816696365	24.066412992671353
82-83	23.212275078699545	26.69090718854917	25.936392708412836	24.16042502433845
84-85	23.147990095388405	26.630864304863934	26.032391807123588	24.188753792624073
86-87	23.16861096750635	26.659765695355354	26.02761446057701	24.14400887656129
88-89	23.29574228643357	26.511957880528037	25.96741450102132	24.22488533201708
90-91	23.130652280962135	26.482929263675437	26.200305179060972	24.18611327630146
92-93	23.333078831363657	26.45314320308998	26.17777029362015	24.036007671926214
94-95	23.370503478311544	26.470594161839823	26.04715499196723	24.111747367881403
96-97	23.24675927695689	26.32920102040725	26.14735765018269	24.276682052453165
98-99	23.4590534231699	25.520225677555246	26.591278900274816	24.429441999000037
100-101	24.29992619007804	12.036567138421164	32.75584776728333	30.907658904217467
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	23641.0
1	11921.5
2	333.5
3	737.5
4	1274.5
5	1782.5
6	2253.5
7	2648.5
8	2992.0
9	3362.0
10	3785.0
11	4202.5
12	4705.0
13	5208.0
14	5724.5
15	6300.5
16	7151.0
17	8348.0
18	9770.0
19	11648.0
20	14014.0
21	17269.0
22	21971.5
23	29073.0
24	38125.0
25	49357.5
26	64396.5
27	86801.5
28	117068.5
29	154565.0
30	206499.5
31	275034.0
32	375966.0
33	511254.0
34	676502.0
35	885263.5
36	1160179.5
37	1506140.5
38	1935022.0
39	2456723.5
40	2987499.0
41	3496668.0
42	4028024.0
43	4491094.5
44	4846848.5
45	5108187.5
46	5214558.0
47	5182858.5
48	4998004.5
49	4713284.5
50	4378710.5
51	4021604.0
52	3605090.0
53	3098686.5
54	2635865.0
55	2289268.5
56	1982943.0
57	1666292.5
58	1382251.0
59	1130880.5
60	945558.0
61	830305.5
62	753168.0
63	680730.5
64	594809.5
65	515517.0
66	455962.0
67	397903.0
68	319470.0
69	243224.5
70	184237.5
71	137197.0
72	103202.0
73	78794.5
74	63502.0
75	46045.5
76	28023.5
77	19355.5
78	13555.5
79	9760.0
80	7972.5
81	6084.0
82	4009.5
83	2697.0
84	2402.5
85	2214.0
86	2078.5
87	1961.0
88	1861.0
89	1748.5
90	1655.5
91	1605.0
92	1537.5
93	1499.0
94	1484.0
95	1475.0
96	1507.0
97	1556.0
98	1717.5
99	2322.5
100	12335.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.07304610093490374
2	0.04190201921183615
3	0.027249513453750394
4	0.027170194344395663
5	0.027089742104907298
6	0.027916927102463757
7	0.026621759359714395
8	0.02726311101535406
9	0.026607028667977088
10-11	0.026643855397320355
12-13	0.026627425010382587
14-15	0.02662345905491485
16-17	0.027409284802593487
18-19	0.02666708456505995
20-21	0.02665462013358992
22-23	0.02876167561709162
24-25	0.03078261321043676
26-27	0.03363016923627153
28-29	0.036330418344733245
30-31	0.0388431344160777
32-33	0.041928647769976665
34-35	0.04439660520104239
36-37	2.051920794837027E-4
38-39	5.362328847401368E-4
40-41	6.700255466796255E-4
42-43	0.0011059689757845798
44-45	8.91717355620742E-4
46-47	5.595357710283024E-4
48-49	5.635195686138649E-4
50-51	0.0010080143090409161
52-53	3.7475611300215545E-4
54-55	3.401823049445549E-5
56-57	7.881133411990824E-5
58-59	1.9278197850023932E-5
60-61	4.5928950272748224E-5
62-63	3.969301490319041E-6
64-65	1.92801030668945E-5
66-67	1.0207464330781457E-5
68-69	1.758015136941326E-5
70-71	2.7221932847404817E-5
72-73	0.0
74-75	1.0776211476866E-5
76-77	4.027073162078549E-5
78-79	5.218408667150587E-5
80-81	2.59798840820683E-4
82-83	0.0014981736661697734
84-85	1.6622065259498976E-4
86-87	0.0012305585995664452
88-89	5.378709640862139E-4
90-91	0.002140319250632241
92-93	9.659365977491714E-4
94-95	0.0010313418721553383
96-97	0.0017649860599343922
98-99	0.0015722821199643624
100-101	3.3831615799207425E-4
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
34-35	40615.0
36-37	2035.0
38-39	2287.0
40-41	2343.0
42-43	2608.0
44-45	2528.0
46-47	2372.0
48-49	2483.0
50-51	2489.0
52-53	2710.0
54-55	2717.0
56-57	2718.0
58-59	2813.0
60-61	2927.0
62-63	2904.0
64-65	3036.0
66-67	3130.0
68-69	3297.0
70-71	3533.0
72-73	3593.0
74-75	3689.0
76-77	3782.0
78-79	4253.0
80-81	4437.0
82-83	4692.0
84-85	5111.0
86-87	5310.0
88-89	6310.0
90-91	16076.0
92-93	10973.0
94-95	34371.0
96-97	629583.0
98-99	7978170.0
100-101	7.9451222E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.13809400400567
#Duplication Level	Percentage of deduplicated	Percentage of total
1	59.99986874732131	16.282820782928077
2	15.245666925297257	8.274766843449541
3	7.688151308265375	6.259253187621748
4	4.520618707376756	4.90723901748228
5	2.729766737740847	3.70403331689095
6	1.83235486327575	2.983597111696456
7	1.3005982332548474	2.470702998085962
8	0.9718593478599729	2.1099528272716475
9	0.7467186701758928	1.8238069319201535
>10	4.355858706265976	21.789562390035606
>50	0.3515447336345134	6.599611457826507
>100	0.22348500965080312	11.737979121095053
>500	0.021260161504223628	3.939475020704809
>1k	0.011694122094133773	5.801243278686993
>5k	4.117451841926066E-4	0.7065037530154848
>10k+	1.4198109799745055E-4	0.6094519612890776
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	1.1331301336389885E-6	2.266260267277977E-6	3.399390400916965E-6	3.399390400916965E-6
2	0.0	1.1331301336389885E-6	9.065041069111908E-6	3.399390400916965E-6	7.93191093547292E-6
3	1.1331301336389885E-6	1.1331301336389885E-6	1.2464431470028872E-5	4.532520534555954E-6	2.152947253914078E-5
4	1.359756160366786E-5	1.1331301336389885E-6	1.586382187094584E-5	4.532520534555954E-6	2.266260267277977E-5
5	2.152947253914078E-5	1.1331301336389885E-6	1.9263212271862802E-5	5.665650668194942E-6	2.606199307369673E-5
6	3.059451360825269E-5	1.1331301336389885E-6	2.719512320733572E-5	6.79878080183393E-6	2.9461383474613698E-5
7	3.626016427644763E-5	1.1331301336389885E-6	3.172764374189168E-5	6.79878080183393E-6	3.172764374189168E-5
8	4.645833547919853E-5	1.1331301336389885E-6	3.172764374189168E-5	6.79878080183393E-6	3.172764374189168E-5
9	5.325711628103245E-5	1.1331301336389885E-6	3.7393294410086614E-5	6.79878080183393E-6	4.0792684811003584E-5
10-11	6.515498268424183E-5	3.399390400916965E-6	4.022611974418408E-5	7.3653458686534245E-6	5.722307174876892E-5
12-13	7.478658882017323E-5	4.532520534555954E-6	6.232215735014437E-5	7.93191093547292E-6	6.572154775106132E-5
14-15	9.461636615885553E-5	4.532520534555954E-6	7.875254428790969E-5	1.0764736269570391E-5	8.101880455518767E-5
16-17	1.0934705789616237E-4	5.099085601375448E-6	1.0198171202750896E-4	1.2464431470028872E-5	8.328506482246565E-5
18-19	1.263440099007472E-4	5.665650668194942E-6	1.1954522909891328E-4	1.2464431470028872E-5	8.78175853570216E-5
20-21	1.3257622563576165E-4	5.665650668194942E-6	1.3484248590303963E-4	1.2464431470028872E-5	9.518293122567502E-5
22-23	1.4957317764034646E-4	5.665650668194942E-6	1.4787348243988798E-4	1.3030996536848367E-5	9.7449191492953E-5
24-25	1.8186738644905765E-4	5.665650668194942E-6	1.5353913310808294E-4	1.4730691737306849E-5	9.971545176023098E-5
26-27	2.2832572192825614E-4	6.232215735014436E-6	1.6430386937765334E-4	1.4730691737306849E-5	1.0311484216114795E-4
28-29	2.815828382092886E-4	9.065041069111908E-6	1.8639990698361358E-4	1.9829777338682297E-5	1.0878049282934289E-4
30-31	3.575025571631008E-4	1.2464431470028872E-5	1.9433181791908652E-4	2.152947253914078E-5	1.2521087976710823E-4
32-33	4.277566254487181E-4	1.4730691737306849E-5	1.9716464325318398E-4	2.436229787323825E-5	1.3257622563576162E-4
34-35	4.8101374172975056E-4	2.266260267277977E-5	2.254928965941587E-4	2.832825334097471E-5	1.4447409203897102E-4
36-37	5.541006353494654E-4	2.266260267277977E-5	2.3569106779690958E-4	2.832825334097471E-5	1.569385235089999E-4
38-39	6.515498268424183E-4	2.266260267277977E-5	2.526880198014944E-4	2.9461383474613698E-5	1.6940295497902877E-4
40-41	7.450330628676349E-4	2.266260267277977E-5	2.6855184167244025E-4	3.059451360825269E-5	1.745020405804042E-4
42-43	8.33417213291476E-4	3.0027948541433193E-5	2.838490984765666E-4	3.059451360825269E-5	1.756351707140432E-4
44-45	9.280335794503315E-4	3.172764374189168E-5	2.8951474914476157E-4	3.6826729343267126E-5	1.9263212271862805E-4
46-47	0.0010277490312105625	3.172764374189168E-5	3.002794854143319E-4	4.419207521192055E-5	1.9829777338682296E-4
48-49	0.0011540930411113098	3.3427338942350156E-5	3.206758278198337E-4	4.532520534555954E-5	2.0962907472321286E-4
50-51	0.0012526753627379018	3.6260164276447624E-5	3.229420880871117E-4	4.645833547919853E-5	2.3455793766327058E-4
52-53	0.0013455920336962988	4.475864027874004E-5	3.314405640894041E-4	5.042429094693498E-5	2.4022358833146554E-4
54-55	0.0014691032182629483	5.212398614739347E-5	3.507037763612669E-4	5.439024641467144E-5	2.4248984859874352E-4
56-57	0.0015977134884309738	5.495681148149094E-5	3.744995091676857E-4	5.835620188240791E-5	2.572205403360504E-4
58-59	0.0017268903236658183	5.665650668194942E-5	3.8866363583817305E-4	6.118902721650538E-5	2.8214940327610813E-4
60-61	0.0018656987650365945	5.7789636815588406E-5	4.1699188917914776E-4	6.118902721650538E-5	2.844156635433861E-4
62-63	0.0020186713330778575	6.0055897082866386E-5	4.6231709452470725E-4	6.118902721650538E-5	2.94613834746137E-4
64-65	0.0021620122949831903	6.175559228332488E-5	4.8497969719748705E-4	6.118902721650538E-5	3.048120059488879E-4
66-67	0.00230705295208898	6.232215735014437E-5	4.889456526652235E-4	6.175559228332488E-5	3.167098723520972E-4
68-69	0.0024758893420011897	6.232215735014437E-5	4.985772588011549E-4	6.402185255060284E-5	3.235086531539312E-4
70-71	0.0026186637388397023	6.345528748378336E-5	4.985772588011549E-4	6.458841761742233E-5	3.3200712915622363E-4
72-73	0.0027875001287519115	6.345528748378336E-5	5.076422998702668E-4	6.572154775106132E-5	3.46171255826711E-4
74-75	0.0030005285938760414	6.572154775106134E-5	5.087754300039058E-4	6.912093815197829E-5	3.546697318290034E-4
76-77	0.0031755971995232647	7.705284908745121E-5	5.167073409393787E-4	6.96875032187978E-5	3.6260164276447624E-4
78-79	0.003287777082753525	8.66844552233826E-5	5.201067313402957E-4	7.195376348607577E-5	3.7676576943496365E-4
80-81	0.003378427493444644	8.951728055748008E-5	5.263389470753101E-4	7.422002375335374E-5	3.818648550363391E-4
82-83	0.0034430159110620663	8.951728055748008E-5	5.303049025430465E-4	7.64862840206317E-5	3.914964611722705E-4
84-85	0.003542731362822297	9.065041069111907E-5	5.35403988144422E-4	7.705284908745121E-5	4.0396089264229935E-4
86-87	0.0035784249620319252	9.404980109203603E-5	5.422027689462559E-4	7.705284908745121E-5	4.1642532411232823E-4
88-89	0.00358409061270012	9.461636615885552E-5	5.456021593471729E-4	7.761941415427071E-5	4.260569302482596E-4
90	0.00358409061270012	9.518293122567502E-5	5.507012449485483E-4	7.81859792210902E-5	4.305894507828156E-4
>>END_MODULE
