##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	SRR21290298_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	71746246
Sequences flagged as poor quality	0
Sequence length	35-101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.039295059423736	37.0	37.0	37.0	37.0	37.0
2	36.04715928969998	37.0	37.0	37.0	37.0	37.0
3	36.13908812734258	37.0	37.0	37.0	37.0	37.0
4	36.11077510591983	37.0	37.0	37.0	37.0	37.0
5	36.084170159927254	37.0	37.0	37.0	37.0	37.0
6	35.99780288156122	37.0	37.0	37.0	37.0	37.0
7	35.951130683548236	37.0	37.0	37.0	37.0	37.0
8	36.03330372156336	37.0	37.0	37.0	37.0	37.0
9	35.97047020411354	37.0	37.0	37.0	37.0	37.0
10-11	36.00649454188864	37.0	37.0	37.0	37.0	37.0
12-13	35.98886876952419	37.0	37.0	37.0	37.0	37.0
14-15	35.97997065937081	37.0	37.0	37.0	37.0	37.0
16-17	35.96170430993699	37.0	37.0	37.0	37.0	37.0
18-19	35.968128757565935	37.0	37.0	37.0	37.0	37.0
20-21	35.958343653269324	37.0	37.0	37.0	37.0	37.0
22-23	35.92646651505641	37.0	37.0	37.0	37.0	37.0
24-25	35.91482359230335	37.0	37.0	37.0	37.0	37.0
26-27	35.88535316537676	37.0	37.0	37.0	37.0	37.0
28-29	35.86539000939506	37.0	37.0	37.0	37.0	37.0
30-31	35.84439493182681	37.0	37.0	37.0	37.0	37.0
32-33	35.81762399108659	37.0	37.0	37.0	37.0	37.0
34-35	35.80202864551269	37.0	37.0	37.0	37.0	37.0
36-37	35.78736159362266	37.0	37.0	37.0	37.0	37.0
38-39	35.770617726465886	37.0	37.0	37.0	37.0	37.0
40-41	35.77337345808688	37.0	37.0	37.0	37.0	37.0
42-43	35.734158307726716	37.0	37.0	37.0	37.0	37.0
44-45	35.71978626185698	37.0	37.0	37.0	37.0	37.0
46-47	35.72330142505107	37.0	37.0	37.0	37.0	37.0
48-49	35.69233475010745	37.0	37.0	37.0	37.0	37.0
50-51	35.67096287283729	37.0	37.0	37.0	37.0	37.0
52-53	35.65591851880608	37.0	37.0	37.0	37.0	37.0
54-55	35.64921642912361	37.0	37.0	37.0	37.0	37.0
56-57	35.61526786622034	37.0	37.0	37.0	37.0	37.0
58-59	35.638244637846725	37.0	37.0	37.0	37.0	37.0
60-61	35.59884810482008	37.0	37.0	37.0	37.0	37.0
62-63	35.58701634087126	37.0	37.0	37.0	37.0	37.0
64-65	35.57373346814629	37.0	37.0	37.0	37.0	37.0
66-67	35.55134189716267	37.0	37.0	37.0	37.0	37.0
68-69	35.51479881941374	37.0	37.0	37.0	37.0	37.0
70-71	35.51513555790612	37.0	37.0	37.0	37.0	37.0
72-73	35.491708456580696	37.0	37.0	37.0	37.0	37.0
74-75	35.50469903268285	37.0	37.0	37.0	37.0	37.0
76-77	35.47746873063076	37.0	37.0	37.0	37.0	37.0
78-79	35.42807156061523	37.0	37.0	37.0	37.0	37.0
80-81	35.421741159303224	37.0	37.0	37.0	37.0	37.0
82-83	35.383848968599395	37.0	37.0	37.0	37.0	37.0
84-85	35.33646407607242	37.0	37.0	37.0	37.0	37.0
86-87	35.29358676143382	37.0	37.0	37.0	37.0	37.0
88-89	35.31911998836295	37.0	37.0	37.0	37.0	37.0
90-91	35.25443576061244	37.0	37.0	37.0	31.0	37.0
92-93	35.295969878021616	37.0	37.0	37.0	37.0	37.0
94-95	35.24128248127111	37.0	37.0	37.0	31.0	37.0
96-97	35.21199966070839	37.0	37.0	37.0	31.0	37.0
98-99	35.20498304579891	37.0	37.0	37.0	25.0	37.0
100-101	35.179324737104196	37.0	37.0	37.0	25.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	16749.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	47.0
12	1265.0
13	8712.0
14	17017.0
15	17944.0
16	17014.0
17	17583.0
18	19689.0
19	24794.0
20	34777.0
21	53939.0
22	80440.0
23	115274.0
24	149922.0
25	190852.0
26	235277.0
27	307225.0
28	400906.0
29	539230.0
30	721525.0
31	1034167.0
32	1511259.0
33	2505336.0
34	4627107.0
35	1.167517E7
36	4.0595304E7
37	6827722.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.20467100707304	16.667638589569105	12.02947418850098	45.098216214856876
2	22.429423069715394	20.16405679016312	31.643388937523593	25.763131202597894
3	23.732690204985378	20.440905821468032	23.2468474487623	32.57955652478429
4	24.962821919931123	25.658966220875453	20.569681250957945	28.808530608235483
5	25.990830305727066	29.50761872700803	22.79911353701605	21.702437430248857
6	21.657662500245195	33.676554136579064	22.95364457910229	21.71213878407345
7	19.051115517481023	22.323172798245185	37.67929480608325	20.946416878190547
8	19.819173279717084	24.39885925638362	29.338976852612237	26.44299061128706
9	20.738177220707087	22.830110224111987	31.280032972767575	25.15167958241335
10-11	22.64179935980843	31.00919877182414	23.668707171628643	22.68029469673879
12-13	22.037432865817514	26.21198912915582	27.195722734920146	24.55485527010652
14-15	22.104136505313868	26.75191622033929	26.801845478162218	24.342101796184625
16-17	22.812978159048043	26.739642759508342	26.153155132955586	24.29422394848803
18-19	22.66024287962197	27.04351057068713	25.920910190254787	24.375336359436112
20-21	22.774014365688306	26.934169324622854	26.1625260768918	24.12929023279704
22-23	22.668576204590458	26.972163457202836	26.182817578549887	24.176442759656815
24-25	22.50514287989577	26.95874822226309	26.25653225232086	24.279576645520283
26-27	22.600403187926748	27.087353703422227	26.213503112051217	24.098739996599807
28-29	22.566280589840943	26.97946072923053	26.110639558742704	24.34361912218582
30-31	22.538141092604246	26.835217361209207	26.205273124361188	24.421368421825353
32-33	22.727601151838062	26.930993146607037	26.11836892359605	24.223036777958846
34-35	22.747967898998915	26.911461992293876	25.956069909423757	24.384500199283448
36-37	22.57782040048019	26.811350358660306	26.109448068415286	24.501381172444216
38-39	22.730439540233558	26.98823675858215	26.07397630074907	24.207347400435218
40-41	22.870485268183273	26.843285697496494	25.954701509116738	24.331527525203494
42-43	22.767804622094463	26.716593326378575	26.194641096606304	24.320960954920665
44-45	22.872984468844738	26.73599535052252	26.19602463037157	24.194995550261172
46-47	22.8844000058265	26.75600429557285	26.03754434870905	24.3220513498916
48-49	22.7164476684265	26.643477668828986	26.15670437746474	24.483370285279772
50-51	22.892197109727928	26.72628300759743	26.057389904954277	24.324129977720368
52-53	23.137795744677174	26.76093333540305	25.83765835837038	24.2636125615494
54-55	22.890825515082764	26.687021695338053	26.061505163455127	24.360647626124052
56-57	22.899560533253844	26.79282571581233	26.082142133829215	24.225471617104606
58-59	23.02655081614091	26.681111577661902	25.984314696829824	24.30802290936736
60-61	22.95609731030786	26.573320002685396	26.027370576283175	24.443212110723568
62-63	23.03355640700336	26.538086321987514	26.119547171567337	24.308810099441793
64-65	23.149412214270917	26.62209812254951	25.911103678417035	24.317385984762538
66-67	23.107296125125156	26.601961367226462	25.96246937506371	24.32827313258468
68-69	23.152528034615752	26.66829625019382	25.94887509176696	24.230300623423467
70-71	23.33873266191624	26.619034742687187	25.787404952768807	24.25482764262777
72-73	23.102856103443305	26.653427510738137	25.915090701937444	24.328625683881118
74-75	23.13887100237899	26.67595469925315	25.913370235853645	24.271804062514214
76-77	23.268361519860992	26.61136142141466	25.7937656938307	24.32651136489365
78-79	23.07693729908506	26.569405243708548	25.95555649945391	24.39810095775248
80-81	23.204211862107407	26.625654886050317	25.883871019418912	24.286262232423365
82-83	23.32474812269472	26.608885478445238	25.743529711348117	24.322836687511916
84-85	23.265216439137397	26.54093187810023	25.85441474366664	24.339436939095723
86-87	23.317386019484307	26.553821905099667	25.872823033247204	24.25596904216882
88-89	23.471989373624567	26.4544238977039	25.751912336171273	24.321674392500263
90-91	23.30706576417686	26.429329665690332	25.914598790451436	24.34900577968137
92-93	23.432760816809918	26.499111142404182	25.866481354374727	24.201646686411177
94-95	23.487568331772422	26.42704197844605	25.798117971482277	24.28727171829925
96-97	23.325735548653913	26.33021268356716	25.883241507886318	24.460810259892604
98-99	23.51473523701927	25.50956076932985	26.360815526564224	24.614888467086658
100-101	24.375347395157856	12.064693977061564	32.52775662029075	31.03220200748983
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	16870.0
1	8512.5
2	259.0
3	570.0
4	982.5
5	1384.5
6	1692.5
7	1893.5
8	2183.5
9	2415.0
10	2629.0
11	2991.5
12	3349.5
13	3563.0
14	3956.5
15	4557.5
16	5108.0
17	5823.5
18	6786.0
19	8019.0
20	9650.0
21	11947.5
22	15131.0
23	19889.0
24	26395.0
25	34869.5
26	46570.5
27	64416.5
28	90007.5
29	121672.0
30	164987.0
31	224151.0
32	307035.0
33	414307.0
34	551351.5
35	737783.5
36	970016.0
37	1242230.5
38	1583613.5
39	1983087.5
40	2384766.5
41	2785526.0
42	3201894.5
43	3562012.0
44	3847345.5
45	4042454.0
46	4141600.5
47	4159462.5
48	4032868.5
49	3774580.0
50	3463393.5
51	3155316.5
52	2850118.5
53	2524288.5
54	2182740.0
55	1888891.0
56	1625413.0
57	1369573.5
58	1152409.5
59	986654.5
60	856892.0
61	754467.0
62	673990.5
63	596114.5
64	534331.5
65	481447.0
66	426659.5
67	370490.0
68	303018.0
69	235316.0
70	187095.5
71	146396.0
72	111549.5
73	87728.0
74	73984.0
75	57406.0
76	37528.0
77	30041.5
78	22429.0
79	15562.0
80	13955.5
81	10554.0
82	5686.0
83	2632.0
84	2255.5
85	2054.0
86	1927.5
87	1765.0
88	1642.0
89	1528.0
90	1391.0
91	1261.0
92	1150.5
93	1114.0
94	1039.5
95	991.0
96	991.5
97	985.0
98	1070.5
99	1434.0
100	7375.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.06953255784281731
2	0.03890099002531784
3	0.024080702424486435
4	0.023906477281055237
5	0.02388557026384349
6	0.024788753407390823
7	0.0233545320266652
8	0.024036100787768046
9	0.023346169219780503
10-11	0.02338658945305654
12-13	0.023368470038139695
14-15	0.023360107231255
16-17	0.024206144527756896
18-19	0.02342004068059533
20-21	0.02340261816625221
22-23	0.02543338643808625
24-25	0.02671568349373987
26-27	0.029108143163337076
28-29	0.031695734993577224
30-31	0.034038714722439974
32-33	0.036854889940861855
34-35	0.03909124388194471
36-37	2.0845895628951032E-4
38-39	5.319667314838731E-4
40-41	6.874609041471867E-4
42-43	0.0010856019974742079
44-45	8.659961708745156E-4
46-47	6.247620665837802E-4
48-49	5.745743861153821E-4
50-51	0.0010180854015639478
52-53	3.842344410835515E-4
54-55	2.719712155707454E-5
56-57	8.57783833871122E-5
58-59	2.1619659076806383E-5
60-61	4.114855033273589E-5
62-63	4.882207911292905E-6
64-65	2.441191624028814E-5
66-67	6.975093830182845E-6
68-69	1.95310032739472E-5
70-71	2.5112284698083233E-5
72-73	0.0
74-75	9.069117207247675E-6
76-77	2.99992283919395E-5
78-79	6.139676724531586E-5
80-81	2.8397499518533306E-4
82-83	0.001553222368085107
84-85	1.8910417340699186E-4
86-87	0.0012763550125709803
88-89	5.478418416195517E-4
90-91	0.0022411237853140493
92-93	9.647516602175369E-4
94-95	0.0011031281019586955
96-97	0.001816613287080034
98-99	0.0017398867464610784
100-101	3.5260696739554344E-4
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
34-35	29061.0
36-37	1719.0
38-39	1885.0
40-41	1736.0
42-43	2026.0
44-45	2052.0
46-47	2025.0
48-49	1980.0
50-51	2191.0
52-53	2212.0
54-55	2403.0
56-57	2353.0
58-59	2455.0
60-61	2638.0
62-63	2547.0
64-65	2665.0
66-67	2685.0
68-69	2806.0
70-71	3008.0
72-73	3194.0
74-75	3296.0
76-77	3387.0
78-79	3730.0
80-81	3822.0
82-83	3710.0
84-85	4222.0
86-87	4410.0
88-89	5537.0
90-91	13027.0
92-93	9427.0
94-95	27921.0
96-97	507794.0
98-99	6506693.0
100-101	6.4575629E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.95918572513946
#Duplication Level	Percentage of deduplicated	Percentage of total
1	62.639065757179324	20.018935361829175
2	15.274794791168075	9.763400072886666
3	7.236215079851248	6.937906250520627
4	3.88864430494546	4.971116222430312
5	2.4259008555590578	3.876490799679332
6	1.6427403665201623	3.1500386683080923
7	1.1512742818548842	2.5755652016063757
8	0.8893690212703854	2.27388077831726
9	0.6511132862311731	1.8728145398491158
>10	3.7416191594888635	21.851997214825104
>50	0.27696303420858603	6.082900585534752
>100	0.1624818422891541	9.868120766428657
>500	0.013452024729049253	2.9234763169900106
>1k	0.006181945078938067	3.3557594951299436
>5k	1.394321492811044E-4	0.2824109258493826
>10k+	4.48174765546407E-5	0.19518679981514736
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	4.181403442348747E-6	0.0	1.811941491684457E-5
2	0.0	5.575204589798329E-6	9.756608032147076E-6	6.969005737247911E-6	2.6482221801542063E-5
3	4.181403442348747E-6	5.575204589798329E-6	9.756608032147076E-6	6.969005737247911E-6	4.460163671838663E-5
4	9.756608032147076E-6	5.575204589798329E-6	1.254421032704624E-5	6.969005737247911E-6	4.5995437865836216E-5
5	2.0907017211743734E-5	5.575204589798329E-6	1.6725613769394988E-5	6.969005737247911E-6	4.73892390132858E-5
6	2.508842065409248E-5	5.575204589798329E-6	2.9269824096441228E-5	6.969005737247911E-6	4.73892390132858E-5
7	2.6482221801542063E-5	5.575204589798329E-6	5.9933449340332036E-5	6.969005737247911E-6	5.017684130818496E-5
8	3.484502868623956E-5	5.575204589798329E-6	6.411485278268078E-5	8.362806884697494E-6	5.2964443603084126E-5
9	3.763263098113872E-5	5.575204589798329E-6	6.829625622502953E-5	9.756608032147076E-6	5.575204589798329E-5
10-11	4.8783040160735384E-5	6.969005737247911E-6	7.80528642571766E-5	1.0453508605871867E-5	5.923654876660724E-5
12-13	6.27210516352312E-5	9.059707458422284E-6	1.094133900747922E-4	1.184730975332145E-5	7.247765966737828E-5
14-15	7.87497648309014E-5	1.0453508605871867E-5	1.6237783367787633E-4	1.3938011474495822E-5	8.223426769952535E-5
16-17	1.0453508605871866E-4	1.1150409179596657E-5	1.9095075720059276E-4	1.3938011474495822E-5	8.571877056814931E-5
18-19	1.247452026967376E-4	1.254421032704624E-5	2.1464537670723568E-4	1.6028713195670195E-5	8.920327343677327E-5
20-21	1.3659251245005905E-4	1.254421032704624E-5	2.439152008036769E-4	1.742251434311978E-5	9.199087573167243E-5
22-23	1.5192432507200448E-4	1.6725613769394988E-5	2.745788260475677E-4	1.811941491684457E-5	9.826298089519556E-5
24-25	1.7143754113629862E-4	1.742251434311978E-5	2.794571300636412E-4	2.0210116638018943E-5	1.024443843375443E-4
26-27	2.125546749860613E-4	2.0210116638018943E-5	2.8503233465343957E-4	2.2300818359193314E-5	1.0523198663244345E-4
28-29	2.571563117044479E-4	2.2300818359193314E-5	2.9060753924323786E-4	2.508842065409248E-5	1.1289789294341616E-4
30-31	3.045455507177337E-4	2.2300818359193314E-5	2.9478894268558664E-4	2.6482221801542063E-5	1.1638239581204012E-4
32-33	3.512378891572947E-4	2.508842065409248E-5	3.024548489965593E-4	2.6482221801542063E-5	1.2056379925438886E-4
34-35	3.986271281705805E-4	2.5785321227817273E-5	3.372998776827989E-4	2.8572923522716434E-5	1.3171420843398552E-4
36-37	4.6831718554305964E-4	2.6482221801542063E-5	3.5611619317336827E-4	2.9269824096441228E-5	1.5053052392455487E-4
38-39	5.428855469316123E-4	2.6482221801542063E-5	3.7005420464786406E-4	2.9269824096441228E-5	1.6725613769394987E-4
40-41	6.118787037303667E-4	2.6482221801542063E-5	3.930519235807822E-4	2.9269824096441228E-5	1.8328485088962008E-4
42-43	6.955067725773415E-4	2.7876022948991644E-5	4.2789695226702173E-4	2.9269824096441228E-5	1.9095075720059276E-4
44-45	7.763472391294174E-4	2.7876022948991644E-5	4.383504608728936E-4	3.3451227538789977E-5	2.1812987957585961E-4
46-47	8.655505125661905E-4	2.7876022948991644E-5	4.676202849693349E-4	3.554192925996435E-5	2.3137099047663065E-4
48-49	9.77751504935882E-4	3.066362524389081E-5	4.794675947226563E-4	3.763263098113872E-5	2.522780076883744E-4
50-51	0.0010767113864048024	3.484502868623956E-5	4.927087056234273E-4	3.9026432128588306E-5	2.787602294899164E-4
52-53	0.0011721867650050987	3.623882983368914E-5	5.087374188190974E-4	4.042023327603789E-5	2.975765449804858E-4
54-55	0.0012802063539324414	3.763263098113872E-5	5.345227400469148E-4	4.251093499721226E-5	3.052424512914585E-4
56-57	0.001374287931385288	3.832953155486351E-5	5.700646693068791E-4	4.320783557093705E-5	3.129083576024312E-4
58-59	0.0014969424323608514	3.97233327023131E-5	5.874871836499989E-4	4.320783557093705E-5	3.2057426391340395E-4
60-61	0.001620990734483864	4.042023327603789E-5	6.341795220895599E-4	4.460163671838663E-5	3.254525679294775E-4
62-63	0.0017345855280010052	4.042023327603789E-5	6.927191702824423E-4	4.460163671838663E-5	3.26846369076927E-4
64-65	0.0018662997364349908	4.042023327603789E-5	7.261703978212324E-4	4.5995437865836216E-5	3.393905794039733E-4
66-67	0.002004286050032499	4.042023327603789E-5	7.331394035584803E-4	4.878304016073538E-5	3.4845028686239553E-4
68-69	0.002121365346418264	4.042023327603789E-5	7.449867133118018E-4	5.2964443603084126E-5	3.6447900005806577E-4
70-71	0.0022461105491150017	4.251093499721226E-5	7.477743156067008E-4	5.7145847045432875E-5	3.763263098113872E-4
72-73	0.002395944172465832	4.3904736144661835E-5	7.693782333921694E-4	5.8539648192882456E-5	3.9096122185960783E-4
74-75	0.002569472415323305	4.529853729211142E-5	7.721658356870686E-4	6.132725048778162E-5	4.0141473046547966E-4
76-77	0.002711640132363162	4.947994073446017E-5	7.777410402768669E-4	6.27210516352312E-5	4.2720005169329697E-4
78-79	0.0028078124115371835	5.366134417680892E-5	7.791348414243165E-4	6.341795220895599E-5	4.453194666101415E-4
80-81	0.0029005001878425803	5.435824475053371E-5	7.798317419980413E-4	6.481175335640557E-5	4.5159157177366465E-4
82-83	0.002972977847509959	5.575204589798329E-5	7.826193442929404E-4	6.690245507757995E-5	4.6413578210071087E-4
84-85	0.0031005106525015956	5.575204589798329E-5	7.87497648309014E-4	6.899315679875431E-5	4.731954895591332E-4
86-87	0.0031576564995470287	5.7145847045432875E-5	7.916790517513627E-4	7.247765966737828E-5	4.815582964438307E-4
88-89	0.0031681100081529003	6.27210516352312E-5	7.951635546199866E-4	7.665906310972702E-5	5.010715125081248E-4
90	0.0031681100081529003	6.829625622502953E-5	7.986480574886107E-4	7.665906310972702E-5	5.115250211139967E-4
>>END_MODULE
