##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	SRR21290306_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	88520848
Sequences flagged as poor quality	0
Sequence length	35-101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.04609197824223	37.0	37.0	37.0	37.0	37.0
2	36.036423318041415	37.0	37.0	37.0	37.0	37.0
3	36.1278439515175	37.0	37.0	37.0	37.0	37.0
4	36.10289050778185	37.0	37.0	37.0	37.0	37.0
5	36.0721478967305	37.0	37.0	37.0	37.0	37.0
6	35.9770420974729	37.0	37.0	37.0	37.0	37.0
7	35.93162479645473	37.0	37.0	37.0	37.0	37.0
8	36.02216564848091	37.0	37.0	37.0	37.0	37.0
9	35.96686257456548	37.0	37.0	37.0	37.0	37.0
10-11	36.00272548225024	37.0	37.0	37.0	37.0	37.0
12-13	35.9819960491115	37.0	37.0	37.0	37.0	37.0
14-15	35.97489181870468	37.0	37.0	37.0	37.0	37.0
16-17	35.95506540447963	37.0	37.0	37.0	37.0	37.0
18-19	35.96619895123463	37.0	37.0	37.0	37.0	37.0
20-21	35.95874140857756	37.0	37.0	37.0	37.0	37.0
22-23	35.92726717891361	37.0	37.0	37.0	37.0	37.0
24-25	35.91336849823219	37.0	37.0	37.0	37.0	37.0
26-27	35.88345097530019	37.0	37.0	37.0	37.0	37.0
28-29	35.8661276832775	37.0	37.0	37.0	37.0	37.0
30-31	35.84608179533029	37.0	37.0	37.0	37.0	37.0
32-33	35.82432501098498	37.0	37.0	37.0	37.0	37.0
34-35	35.80665846084078	37.0	37.0	37.0	37.0	37.0
36-37	35.801408676248855	37.0	37.0	37.0	37.0	37.0
38-39	35.789497024688714	37.0	37.0	37.0	37.0	37.0
40-41	35.795273911325836	37.0	37.0	37.0	37.0	37.0
42-43	35.754765302171506	37.0	37.0	37.0	37.0	37.0
44-45	35.74149535263997	37.0	37.0	37.0	37.0	37.0
46-47	35.74829805459004	37.0	37.0	37.0	37.0	37.0
48-49	35.72332971976506	37.0	37.0	37.0	37.0	37.0
50-51	35.70503356610881	37.0	37.0	37.0	37.0	37.0
52-53	35.69428633335187	37.0	37.0	37.0	37.0	37.0
54-55	35.69029472292816	37.0	37.0	37.0	37.0	37.0
56-57	35.65397710392094	37.0	37.0	37.0	37.0	37.0
58-59	35.67687549860724	37.0	37.0	37.0	37.0	37.0
60-61	35.64280354015132	37.0	37.0	37.0	37.0	37.0
62-63	35.63081403785021	37.0	37.0	37.0	37.0	37.0
64-65	35.61794875834147	37.0	37.0	37.0	37.0	37.0
66-67	35.60003839626374	37.0	37.0	37.0	37.0	37.0
68-69	35.56179010088557	37.0	37.0	37.0	37.0	37.0
70-71	35.57268032810451	37.0	37.0	37.0	37.0	37.0
72-73	35.550942603083655	37.0	37.0	37.0	37.0	37.0
74-75	35.562937668575316	37.0	37.0	37.0	37.0	37.0
76-77	35.537655660848976	37.0	37.0	37.0	37.0	37.0
78-79	35.48764247061257	37.0	37.0	37.0	37.0	37.0
80-81	35.484262837626446	37.0	37.0	37.0	37.0	37.0
82-83	35.45034030659349	37.0	37.0	37.0	37.0	37.0
84-85	35.405493782819335	37.0	37.0	37.0	37.0	37.0
86-87	35.36357427218816	37.0	37.0	37.0	37.0	37.0
88-89	35.3921785879451	37.0	37.0	37.0	37.0	37.0
90-91	35.32643823828448	37.0	37.0	37.0	37.0	37.0
92-93	35.37575089464441	37.0	37.0	37.0	37.0	37.0
94-95	35.32472121343904	37.0	37.0	37.0	37.0	37.0
96-97	35.30147056401269	37.0	37.0	37.0	37.0	37.0
98-99	35.298006854902354	37.0	37.0	37.0	37.0	37.0
100-101	35.27648513130096	37.0	37.0	37.0	37.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	32594.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	42.0
12	1972.0
13	13647.0
14	25622.0
15	25777.0
16	22683.0
17	22966.0
18	25897.0
19	31795.0
20	43600.0
21	68016.0
22	102559.0
23	145758.0
24	188373.0
25	234237.0
26	283861.0
27	364863.0
28	465710.0
29	622616.0
30	832622.0
31	1192228.0
32	1747179.0
33	2922405.0
34	5465983.0
35	1.4134399E7
36	5.0786216E7
37	8717228.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.629872390452896	15.753012917613663	12.37490504049158	45.24220965144186
2	22.582802914819663	19.551085337647006	31.762187699608766	26.103924047924558
3	24.056012010632948	20.10096375884018	23.26655444227079	32.57646978825608
4	25.23896449504235	25.252603674631622	20.6697240624115	28.838707767914528
5	26.122127242481074	29.205214700939596	22.96183249481999	21.71082556175934
6	21.497732135050708	33.868692375061634	22.927323390920456	21.7062520989672
7	18.987714560000928	21.709416244144432	38.241783148525165	21.061086047329475
8	20.109436482431743	23.966110263761475	29.23240745976875	26.692045794038034
9	21.086050823039752	22.075548265146562	31.47032634941951	25.368074562394177
10-11	22.92150443390652	30.506056280643275	23.93157672364099	22.640862561809215
12-13	22.19924151498784	25.707808501322237	27.57711462998443	24.515835353705494
14-15	22.22766199086081	26.320638051335195	27.034299546277	24.417400411526998
16-17	23.094529301282833	26.308250466496684	26.455904655775704	24.141315576444782
18-19	22.8624444036719	26.72940878311842	25.943803995867448	24.46434281734223
20-21	22.89193322569676	26.52933340497377	26.222289646151303	24.356443723178167
22-23	22.74642365330386	26.678217670055055	26.34919201102166	24.226166665619424
24-25	22.78814112649683	26.442541151595396	26.467749342945467	24.30156837896231
26-27	22.91084297206726	26.711773113105146	26.27029164945578	24.107092265371815
28-29	22.738623879182793	26.47994912270563	26.348399357962144	24.43302764014943
30-31	22.781803124642515	26.16889828909895	26.502034678723014	24.547263907535523
32-33	22.97853541337648	26.411640166941876	26.318049958023998	24.291774461657646
34-35	22.986836493097513	26.47474205571107	26.191660012134033	24.346761439057385
36-37	22.698179281706313	26.42187426543397	26.406563924097288	24.473382528762432
38-39	23.0564114977682	26.560105026325083	26.395544710003566	23.98793876590315
40-41	23.208319604632404	26.412106378168588	25.980245637664417	24.399328379534587
42-43	22.945897907717736	26.5099034098429	26.202699631711678	24.341499050727684
44-45	23.099773939479267	26.402068947596813	26.365030269027162	24.133126843896758
46-47	23.007105782281727	26.160235232461105	26.381441864067835	24.451217121189337
48-49	22.82060527905618	25.993062881975444	26.5343484073428	24.651983431625574
50-51	23.082942059870636	25.991366488721045	26.37552223969758	24.55016921171074
52-53	23.311964228483333	26.28797594775402	26.06123708094839	24.338822742814255
54-55	22.850238214554626	26.25802175106574	26.383826470148676	24.50791356423096
56-57	22.988061639444318	26.428684719656616	26.265021320711895	24.318232320187168
58-59	22.983361449864084	26.326587808312247	26.215401662495886	24.474649079327783
60-61	23.061680516018242	26.182778467951017	26.198420229568438	24.557120786462303
62-63	23.156897610301534	26.041227730858445	26.267182612433754	24.534692046406263
64-65	23.19247807163721	26.240437538935534	25.997162645747096	24.569921743680155
66-67	23.262798025069724	26.156151106982083	26.082459206075274	24.498591661872915
68-69	23.194219364549056	26.276063268937712	26.281385890876546	24.248331475636682
70-71	23.566134217085775	26.216146360109217	26.060574404252772	24.157145018552235
72-73	23.294967165871043	26.09642528174066	26.06347197392508	24.545135578463217
74-75	23.391662069392076	26.2634237180393	26.067906720870198	24.277007491698424
76-77	23.366599858233226	26.262825529366612	26.04604857124979	24.324526041150367
78-79	23.16739181467877	26.330617508209244	25.902960890952002	24.599029786159985
80-81	23.390113064352388	26.29312827871687	25.934972320286242	24.3817863366445
82-83	23.458373358534963	26.367623142706297	25.685136443094986	24.488867055663754
84-85	23.34276633508471	26.396497224197013	25.81511740817344	24.445619032544837
86-87	23.317873126018775	26.345116273838936	25.856088510827725	24.480922089314564
88-89	23.486504371407566	26.107284521016638	25.790811034394878	24.615400073180915
90-91	23.39759052096104	26.10240800795382	26.09619210742452	24.40380936366062
92-93	23.723617964816555	26.03066386557751	26.00667702046657	24.239041149139368
94-95	23.507964464772993	26.1160552628024	26.028195546213333	24.347784726211273
96-97	23.53078511185637	25.868663855707503	26.093365529213585	24.50718550322254
98-99	23.707657240756415	25.087365391795796	26.500718099511282	24.704259267936507
100-101	24.663020182798427	11.640345782668806	32.35521275012594	31.341421284406827
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	32713.0
1	16448.5
2	302.5
3	647.5
4	1125.0
5	1611.5
6	2035.5
7	2425.5
8	2804.0
9	3164.5
10	3483.0
11	3830.5
12	4227.0
13	4543.0
14	4866.5
15	5320.0
16	5992.0
17	6772.5
18	7567.5
19	8765.5
20	10293.5
21	12976.5
22	17385.0
23	23155.0
24	29493.5
25	37383.0
26	46798.0
27	62988.0
28	84458.0
29	108755.5
30	147084.0
31	197421.0
32	282302.5
33	401942.5
34	537004.0
35	698826.0
36	943342.5
37	1283961.5
38	1695659.0
39	2263133.5
40	2819860.0
41	3301006.0
42	3832709.5
43	4375054.5
44	4813046.5
45	5113744.5
46	5263753.0
47	5267087.5
48	5134185.5
49	4948082.5
50	4680611.0
51	4356525.0
52	3900233.0
53	3252311.5
54	2722852.5
55	2375605.0
56	2094831.0
57	1796816.0
58	1514916.0
59	1222273.0
60	1001737.5
61	890498.5
62	831451.0
63	765115.0
64	640450.0
65	523591.5
66	458693.5
67	407311.5
68	333237.0
69	256034.0
70	188174.5
71	137424.0
72	105529.5
73	83704.0
74	71831.0
75	54761.5
76	35395.0
77	29342.0
78	22850.5
79	17157.0
80	15728.5
81	11718.0
82	6535.0
83	3667.5
84	3257.0
85	2926.0
86	2673.0
87	2448.5
88	2207.0
89	2041.0
90	1903.5
91	1769.0
92	1618.5
93	1494.0
94	1469.5
95	1437.5
96	1399.5
97	1458.5
98	1617.5
99	2152.0
100	11622.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.08352721609716166
2	0.052208040302551106
3	0.03756742140563317
4	0.037416044636174296
5	0.03737989495988561
6	0.03827008073849451
7	0.036833131106019226
8	0.03747365818275939
9	0.03682183433217901
10-11	0.0368579840084677
12-13	0.0368421685250914
14-15	0.036835955299479285
16-17	0.037673046241039176
18-19	0.03688679078176025
20-21	0.03688001271745612
22-23	0.03924499232090501
24-25	0.0425944857645286
26-27	0.04660540531649674
28-29	0.05020851133283314
30-31	0.05329253059121169
32-33	0.056546001457193455
34-35	0.05911149879630615
36-37	1.8481569509140534E-4
38-39	5.052882141930983E-4
40-41	6.607367624116815E-4
42-43	0.0011005033590448675
44-45	8.868719482617558E-4
46-47	5.844815149552851E-4
48-49	5.833685657987256E-4
50-51	9.875720418117539E-4
52-53	3.7875878022916934E-4
54-55	2.487442935797741E-5
56-57	8.819373082666172E-5
58-59	1.8091547118100218E-5
60-61	4.523025964752816E-5
62-63	5.088564284598719E-6
64-65	1.6962447171801142E-5
66-67	8.4815114161907E-6
68-69	1.809453640958019E-5
70-71	2.883924824901232E-5
72-73	0.0
74-75	7.917315761672893E-6
76-77	3.393280305109287E-5
78-79	5.938509147119573E-5
80-81	2.8393169080886363E-4
82-83	0.001558881246813584
84-85	1.7479071272796782E-4
86-87	0.0012524627241297318
88-89	5.227423382139194E-4
90-91	0.00221506567726312
92-93	9.546658501170654E-4
94-95	0.0010759140930195028
96-97	0.0017554775688328817
98-99	0.0016873589506704962
100-101	3.6457038190722263E-4
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
34-35	53824.0
36-37	2096.0
38-39	2509.0
40-41	2255.0
42-43	2590.0
44-45	2522.0
46-47	2530.0
48-49	2648.0
50-51	2462.0
52-53	2560.0
54-55	2538.0
56-57	2519.0
58-59	2743.0
60-61	2690.0
62-63	2987.0
64-65	2975.0
66-67	3107.0
68-69	3239.0
70-71	3600.0
72-73	3745.0
74-75	3738.0
76-77	3905.0
78-79	4358.0
80-81	4716.0
82-83	5226.0
84-85	5508.0
86-87	5621.0
88-89	6802.0
90-91	15976.0
92-93	11537.0
94-95	33798.0
96-97	637489.0
98-99	7951858.0
100-101	7.9724177E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.790996005495646
#Duplication Level	Percentage of deduplicated	Percentage of total
1	63.16505993385522	13.764295687055322
2	14.408737656007883	6.279614894126049
3	6.907860932884567	4.515875099850211
4	3.858331085146389	3.363075090572185
5	2.4614276986500925	2.681848057455025
6	1.6580997948451661	2.1678987603710502
7	1.1443813148882205	1.7456046063045147
8	0.8536475145250506	1.4881463663293333
9	0.6901580335795284	1.3535307857603012
>10	4.105881165163602	16.89167367506673
>50	0.3785813610158435	5.766090142469152
>100	0.2929165699207011	12.918177080524393
>500	0.03841928315199938	5.8285049185671935
>1k	0.03292386737148997	14.258375030517326
>5k	0.002671610990859915	3.8824731966198014
>10k+	9.021780034494265E-4	3.094816608411084
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	2.259354768042891E-6	0.0	1.1296773840214455E-6
2	1.1296773840214455E-6	0.0	2.259354768042891E-6	0.0	1.1296773840214455E-6
3	1.1296773840214455E-5	0.0	2.259354768042891E-6	2.259354768042891E-6	1.2426451224235899E-5
4	1.3556128608257345E-5	0.0	2.259354768042891E-6	2.259354768042891E-6	1.3556128608257345E-5
5	2.5982579832493244E-5	0.0	7.907741688150118E-6	2.259354768042891E-6	1.8074838144343128E-5
6	4.066838582477204E-5	0.0	7.907741688150118E-6	2.259354768042891E-6	1.8074838144343128E-5
7	4.066838582477204E-5	0.0	9.037419072171564E-6	2.259354768042891E-6	1.8074838144343128E-5
8	4.8576127512922154E-5	0.0	9.037419072171564E-6	2.259354768042891E-6	2.033419291238602E-5
9	4.9705804896943597E-5	0.0	1.1296773840214455E-5	2.259354768042891E-6	2.711225721651469E-5
10-11	6.100257873715806E-5	1.1296773840214455E-6	1.863967683635385E-5	2.8241934600536134E-6	3.106612806058975E-5
12-13	7.455870734541541E-5	1.1296773840214455E-6	2.3723225064450353E-5	3.389032152064336E-6	4.123322451678276E-5
14-15	9.20687067977478E-5	3.389032152064336E-6	3.5584837596675534E-5	3.389032152064336E-6	4.575193405286854E-5
16-17	1.1014354494209093E-4	3.389032152064336E-6	4.5187095360857814E-5	3.389032152064336E-6	4.7446450128900705E-5
18-19	1.2200515747431611E-4	3.389032152064336E-6	5.535419181705083E-5	3.389032152064336E-6	4.9705804896943597E-5
20-21	1.270887057024126E-4	3.389032152064336E-6	6.721580434927601E-5	3.389032152064336E-6	5.874322396911516E-5
22-23	1.3782064085061637E-4	3.389032152064336E-6	7.738290080546901E-5	3.389032152064336E-6	6.1002578737158053E-5
24-25	1.6323838199109887E-4	3.953870844075059E-6	8.020709426552263E-5	3.389032152064336E-6	6.1002578737158053E-5
26-27	1.931748326676672E-4	6.2132256121179496E-6	8.754999726166203E-5	5.083548228096505E-6	6.269709481319022E-5
28-29	2.2988934764836414E-4	7.907741688150118E-6	8.924451333769419E-5	5.6483869201072276E-6	6.834548173329744E-5
30-31	2.711225721651469E-4	8.472580380160841E-6	9.150386810573708E-5	5.6483869201072276E-6	7.681806211345829E-5
32-33	3.0388321630176883E-4	1.3556128608257345E-5	9.319838418176925E-5	6.2132256121179496E-6	8.020709426552262E-5
34-35	3.3720869913040144E-4	1.4120967300268068E-5	1.0505999671399443E-4	6.778064304128672E-6	8.642031987764057E-5
36-37	3.8070127841522707E-4	1.468580599227879E-5	1.095787062500802E-4	7.907741688150118E-6	8.811483595367274E-5
38-39	4.3831482500032086E-4	1.468580599227879E-5	1.1974580270627321E-4	7.907741688150118E-6	1.0054128717790865E-4
40-41	4.976228876614467E-4	1.6945160760321682E-5	1.265238670104019E-4	9.037419072171564E-6	1.1070838363410166E-4
42-43	5.495880473264332E-4	2.033419291238602E-5	1.491174146908308E-4	1.016709645619301E-5	1.1579193186219815E-4
44-45	6.08896109987559E-4	2.0899031604396743E-5	1.5363612422691659E-4	1.016709645619301E-5	1.1974580270627321E-4
46-47	6.631206244205885E-4	2.2028708988418186E-5	1.5702515637898093E-4	1.1296773840214455E-5	1.2256999616632683E-4
48-49	7.450222347621432E-4	2.259354768042891E-5	1.7058128498723826E-4	1.1296773840214455E-5	1.2934806047045552E-4
50-51	8.082841682673442E-4	2.315838637243963E-5	1.7171096237125972E-4	1.1296773840214455E-5	1.3782064085061637E-4
52-53	8.828428756127596E-4	2.3723225064450353E-5	1.734054784472919E-4	1.1296773840214455E-5	1.4233935038670214E-4
54-55	9.63614808570293E-4	2.3723225064450353E-5	1.7792418798337766E-4	1.1296773840214455E-5	1.4459870515474502E-4
56-57	0.0010511648058319548	2.4288063756461077E-5	1.8696160705554922E-4	1.1296773840214455E-5	1.643680593751203E-4
58-59	0.0011494467382418207	2.4852902448471798E-5	1.9260999397565645E-4	1.1296773840214455E-5	1.818780588274527E-4
60-61	0.0012375615741954935	2.4852902448471798E-5	2.1237934819603175E-4	1.1296773840214455E-5	1.8752644574755994E-4
62-63	0.0013251115714571554	2.541774114048252E-5	2.3723225064450353E-4	1.2426451224235899E-5	1.8809128443957068E-4
64-65	0.0014284770520951178	2.5982579832493244E-5	2.530477340208038E-4	1.2426451224235899E-5	1.892209618235921E-4
66-67	0.0015290183392730264	2.5982579832493244E-5	2.5813128224890024E-4	1.2426451224235899E-5	1.9543418743571005E-4
68-69	0.0016227815621468063	2.5982579832493244E-5	2.705577334731362E-4	1.2426451224235899E-5	1.9995289697179584E-4
70-71	0.0017244525267087365	2.5982579832493244E-5	2.711225721651469E-4	1.3556128608257345E-5	2.1407386427206392E-4
72-73	0.001847587361567074	2.5982579832493244E-5	2.779006364692756E-4	1.4120967300268068E-5	2.2254644465222474E-4
74-75	0.001963379293429272	2.5982579832493244E-5	2.79030313853297E-4	1.468580599227879E-5	2.236761220362462E-4
76-77	0.0020650502579912023	2.711225721651469E-5	2.818545073133506E-4	1.468580599227879E-5	2.287596702643427E-4
78-79	0.0021458221909487355	2.711225721651469E-5	2.8467870077340427E-4	1.468580599227879E-5	2.3779708933651427E-4
80-81	0.0022248996078302365	2.767709590852541E-5	2.886325716174793E-4	1.468580599227879E-5	2.4457515364064293E-4
82-83	0.0022926802508715233	2.8241934600536135E-5	2.9428095853758655E-4	1.468580599227879E-5	2.530477340208038E-4
84-85	0.0023813599255172067	2.8241934600536135E-5	2.987996680736723E-4	1.468580599227879E-5	2.5982579832493246E-4
86-87	0.0024225931500339894	2.937161198455758E-5	3.01623861533726E-4	1.468580599227879E-5	2.5982579832493246E-4
88-89	0.0024288063756461075	2.937161198455758E-5	3.027535389177474E-4	1.5250644684289513E-5	2.6773354001308257E-4
90	0.0024288063756461075	3.0501289368579027E-5	3.027535389177474E-4	1.5815483376300236E-5	2.711225721651469E-4
>>END_MODULE
