##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	SRR21290307_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	80980784
Sequences flagged as poor quality	0
Sequence length	35-101
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.0426939309454	37.0	37.0	37.0	37.0	37.0
2	36.18388537705439	37.0	37.0	37.0	37.0	37.0
3	36.29653495327978	37.0	37.0	37.0	37.0	37.0
4	36.32851640458309	37.0	37.0	37.0	37.0	37.0
5	36.34740318147574	37.0	37.0	37.0	37.0	37.0
6	36.31585416609452	37.0	37.0	37.0	37.0	37.0
7	36.27992663049545	37.0	37.0	37.0	37.0	37.0
8	36.3378342447265	37.0	37.0	37.0	37.0	37.0
9	36.316473028959564	37.0	37.0	37.0	37.0	37.0
10-11	36.33836539542517	37.0	37.0	37.0	37.0	37.0
12-13	36.320017839046855	37.0	37.0	37.0	37.0	37.0
14-15	36.315059848025186	37.0	37.0	37.0	37.0	37.0
16-17	36.31472592930194	37.0	37.0	37.0	37.0	37.0
18-19	36.312787426212125	37.0	37.0	37.0	37.0	37.0
20-21	36.30550190153753	37.0	37.0	37.0	37.0	37.0
22-23	36.29348429746	37.0	37.0	37.0	37.0	37.0
24-25	36.26323941862553	37.0	37.0	37.0	37.0	37.0
26-27	36.22014301565665	37.0	37.0	37.0	37.0	37.0
28-29	36.20591669006316	37.0	37.0	37.0	37.0	37.0
30-31	36.19891531921944	37.0	37.0	37.0	37.0	37.0
32-33	36.19413115956003	37.0	37.0	37.0	37.0	37.0
34-35	36.181170146488085	37.0	37.0	37.0	37.0	37.0
36-37	36.19481450526992	37.0	37.0	37.0	37.0	37.0
38-39	36.18912717716786	37.0	37.0	37.0	37.0	37.0
40-41	36.189607234549875	37.0	37.0	37.0	37.0	37.0
42-43	36.17275290922165	37.0	37.0	37.0	37.0	37.0
44-45	36.171017532043095	37.0	37.0	37.0	37.0	37.0
46-47	36.16784336687998	37.0	37.0	37.0	37.0	37.0
48-49	36.14383824789875	37.0	37.0	37.0	37.0	37.0
50-51	36.1374702482789	37.0	37.0	37.0	37.0	37.0
52-53	36.139780189479836	37.0	37.0	37.0	37.0	37.0
54-55	36.130159404181825	37.0	37.0	37.0	37.0	37.0
56-57	36.11728862896942	37.0	37.0	37.0	37.0	37.0
58-59	36.10657825200788	37.0	37.0	37.0	37.0	37.0
60-61	36.10343597454575	37.0	37.0	37.0	37.0	37.0
62-63	36.09216763338533	37.0	37.0	37.0	37.0	37.0
64-65	36.07733779693081	37.0	37.0	37.0	37.0	37.0
66-67	36.07544710477388	37.0	37.0	37.0	37.0	37.0
68-69	36.059863490075166	37.0	37.0	37.0	37.0	37.0
70-71	36.04146486121341	37.0	37.0	37.0	37.0	37.0
72-73	36.02022783301585	37.0	37.0	37.0	37.0	37.0
74-75	36.015377585266336	37.0	37.0	37.0	37.0	37.0
76-77	36.00293533773815	37.0	37.0	37.0	37.0	37.0
78-79	35.9895346686159	37.0	37.0	37.0	37.0	37.0
80-81	35.96149602006811	37.0	37.0	37.0	37.0	37.0
82-83	35.97531764474505	37.0	37.0	37.0	37.0	37.0
84-85	35.95912154796142	37.0	37.0	37.0	37.0	37.0
86-87	35.97240509120411	37.0	37.0	37.0	37.0	37.0
88-89	35.94832652803299	37.0	37.0	37.0	37.0	37.0
90-91	35.92979245758858	37.0	37.0	37.0	37.0	37.0
92-93	35.91970454440781	37.0	37.0	37.0	37.0	37.0
94-95	35.892946456206985	37.0	37.0	37.0	37.0	37.0
96-97	35.89545055281501	37.0	37.0	37.0	37.0	37.0
98-99	35.8763138980627	37.0	37.0	37.0	37.0	37.0
100-101	35.85042114600024	37.0	37.0	37.0	37.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	62886.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	10.0
15	93.0
16	452.0
17	1224.0
18	2230.0
19	3062.0
20	4371.0
21	7196.0
22	13280.0
23	26930.0
24	51114.0
25	91005.0
26	147131.0
27	236090.0
28	356483.0
29	525966.0
30	718889.0
31	982714.0
32	1299811.0
33	1866282.0
34	2965885.0
35	6950335.0
36	4.8217414E7
37	1.6449931E7
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.786713112685078	16.47313046356933	12.543163299535554	47.19699312421004
2	21.877146605022997	19.249649529207474	31.143272272121315	27.72993159364822
3	23.745369249357644	19.287307296367313	23.060463737537958	33.90685971673709
4	24.45346329815595	23.806715142469844	21.123463220052088	30.61635833932212
5	26.05572173640862	27.973593618991387	23.65845263393377	22.31223201066622
6	21.898540913655467	32.841630414077244	22.876192359816365	22.383636312450925
7	19.186814516610404	22.10377115826711	37.67503698625488	21.034377338867603
8	20.066147788465784	24.27011759499734	29.09504890994573	26.568685706591143
9	20.75676436273133	22.261914719087606	31.570725714371022	25.410595203810043
10-11	22.697637301453383	30.006525256007023	24.159816904784158	23.136020537755442
12-13	22.14723453925323	25.63349424811622	27.367629489904527	24.85164172272602
14-15	22.223043151066346	26.119966284561535	26.938399661538114	24.718590902834002
16-17	22.906303731357113	26.05249112305404	26.41848867255766	24.622716473031183
18-19	22.705256430465283	26.51369857130843	25.905271458071404	24.87577354015488
20-21	22.793351424474206	26.350680404982707	26.163481963431952	24.692486207111138
22-23	22.655974338531117	26.376988293946972	26.336740551248432	24.630296816273482
24-25	22.644997456899105	26.18147383360786	26.451642966269723	24.721885743223314
26-27	22.83100136909745	26.419928808909937	26.24918772404789	24.499882097944724
28-29	22.702875016273623	26.231206668503866	26.295914327489594	24.77000398773292
30-31	22.724236010971893	25.921991221834627	26.43223809686744	24.92153467032604
32-33	22.96384771709851	26.135526022167443	26.263509319755414	24.637116940978636
34-35	22.903126227310842	26.181813303213563	26.147481274889923	24.76757919458567
36-37	22.59027295934192	26.150009054014266	26.335528902439282	24.92418908420453
38-39	23.03502301023927	26.252681227866344	26.29412217799168	24.418173583902703
40-41	23.13882608811943	26.16263246191426	25.944668925368457	24.753872524597853
42-43	22.91160303681119	26.26089848903336	26.162277503702185	24.665220970453262
44-45	23.09511232609161	26.211136574458386	26.29408293929474	24.39966816015527
46-47	23.02885244315844	25.94994539333059	26.2621001346884	24.759102028822575
48-49	22.81843953705524	25.824943058465166	26.400356448705303	24.95626095577429
50-51	23.030659313051714	25.861481704879825	26.255601417774603	24.852257564293858
52-53	23.264788990306972	26.089180667317958	25.974716496290785	24.67131384608428
54-55	22.82577449577097	26.07537237699089	26.27974368975349	24.819109437484652
56-57	23.00843986291338	26.261569893801664	26.171997000948167	24.557993242336796
58-59	22.985222270073663	26.103601945887476	26.142196951695457	24.7689788323434
60-61	22.990032969598587	26.002016908994	26.11251410490058	24.895436016506828
62-63	23.127216640434177	25.900674258864324	26.20376246309053	24.768346637610968
64-65	23.15811959567194	26.086164284724028	25.931042110999215	24.824674008604816
66-67	23.15174558617358	25.961115698458027	26.051411145062886	24.83572757030551
68-69	23.161918903054993	26.101865396196093	26.185506335283108	24.550709365465803
70-71	23.504432637748707	26.02223549213013	26.0159423280098	24.457389542111358
72-73	23.250953491909687	25.939026741097575	25.96333658251739	24.846683184475353
74-75	23.392639311879837	26.016497678356355	26.067438557595263	24.52342445216855
76-77	23.369519134168097	25.989272614848836	26.02116385845605	24.620044392527017
78-79	23.169867059611516	26.046903673584726	25.91876467208972	24.864464594714043
80-81	23.426300562273425	26.043763034200502	25.908803265289894	24.621133138236186
82-83	23.430099312452253	26.15282054104155	25.645425824205553	24.771654322300645
84-85	23.251528152102175	26.160689931376492	25.8261718388649	24.761610077656435
86-87	23.33402380352973	26.158632106911128	25.80188965307561	24.70545443648353
88-89	23.46536443033333	25.876371427048873	25.76537508760961	24.892889055008187
90-91	23.357882612531654	25.86464829888156	26.049741830098405	24.727727258488382
92-93	23.672052889349118	25.82254756885598	25.997965197570522	24.50743434422438
94-95	23.504049822928515	25.89659793789942	25.962434286765234	24.636917952406833
96-97	23.472992833836205	25.717385279864153	26.055229414812032	24.75439247148761
98-99	23.724773446337778	24.879426929614617	26.484770076551694	24.911029547495907
100-101	24.548072489758702	11.536036573948552	32.30682432520685	31.6090666110859
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	63102.0
1	31595.5
2	97.0
3	125.5
4	159.5
5	188.0
6	203.0
7	223.5
8	236.5
9	242.0
10	257.5
11	303.0
12	371.0
13	460.0
14	583.0
15	800.5
16	1192.5
17	1676.0
18	2321.5
19	3237.0
20	4125.5
21	5463.0
22	8442.0
23	12882.0
24	17985.0
25	25061.5
26	31259.0
27	40163.5
28	55669.5
29	72922.5
30	99644.5
31	136897.0
32	201030.0
33	295691.5
34	408618.5
35	549541.0
36	766723.0
37	1073610.5
38	1443067.5
39	1940087.5
40	2441682.5
41	2897083.0
42	3409446.5
43	3953127.5
44	4397183.5
45	4722642.5
46	4898451.0
47	4908480.5
48	4809581.0
49	4638461.5
50	4359824.0
51	4027840.0
52	3614495.0
53	3065803.5
54	2595016.0
55	2273139.5
56	2010679.5
57	1713522.0
58	1420894.5
59	1141761.0
60	946431.0
61	842518.5
62	780526.0
63	725289.5
64	621438.5
65	516859.5
66	453691.5
67	395004.5
68	319639.5
69	245177.5
70	182027.0
71	133022.5
72	100716.5
73	79561.0
74	68593.5
75	50038.0
76	28294.5
77	22249.0
78	15988.0
79	9851.0
80	8060.5
81	5599.0
82	2272.5
83	439.0
84	327.5
85	285.5
86	212.5
87	155.5
88	140.0
89	119.5
90	100.0
91	90.0
92	77.0
93	82.0
94	103.5
95	141.0
96	276.5
97	691.0
98	1963.0
99	2277.5
100	886.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.11365906262404177
2	0.08773192415622946
3	0.07948428851960732
4	0.0776579293181454
5	0.07769497514373287
6	0.07765545959643957
7	0.07765545959643957
8	0.07765545959643957
9	0.0776579293181454
10-11	0.07765545959643957
12-13	0.07767583480051268
14-15	0.07774375214742302
16-17	0.07766348619198352
18-19	0.07784439330693563
20-21	0.07768447882648309
22-23	0.0780462930563873
24-25	0.08002454014275781
26-27	0.08170333347229634
28-29	0.08352845781290534
30-31	0.08492693772883206
32-33	0.08623712509377533
34-35	0.08756707023236525
36-37	1.297754780409509E-4
38-39	2.3483487950059964E-5
40-41	2.9045810935588375E-5
42-43	3.4731889067260966E-4
44-45	8.466808500747424E-5
46-47	1.6748493460652704E-4
48-49	1.5018258586935016E-4
50-51	6.365866731853143E-5
52-53	1.0445153724633967E-4
54-55	4.017446869373315E-5
56-57	0.0
58-59	0.0
60-61	6.181073420910202E-7
62-63	6.1812220063272476E-6
64-65	3.708823666092825E-6
66-67	6.799681290283785E-6
68-69	0.0
70-71	1.8545669951725065E-6
72-73	9.273137539936389E-6
74-75	0.0
76-77	1.8547530978642667E-6
78-79	0.0
80-81	6.182987047415009E-7
82-83	5.070271740348268E-5
84-85	6.801900191204506E-6
86-87	8.039029015898181E-6
88-89	2.640657431926882E-4
90-91	4.885918624196563E-5
92-93	9.89731921026296E-6
94-95	5.877271848606525E-4
96-97	2.291988479400438E-5
98-99	2.206181948741947E-5
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
34-35	71583.0
36-37	981.0
38-39	1243.0
40-41	1180.0
42-43	1342.0
44-45	1349.0
46-47	1249.0
48-49	1268.0
50-51	1449.0
52-53	1583.0
54-55	1590.0
56-57	1629.0
58-59	1750.0
60-61	1935.0
62-63	1978.0
64-65	2004.0
66-67	2245.0
68-69	2363.0
70-71	2657.0
72-73	2631.0
74-75	2777.0
76-77	2865.0
78-79	3142.0
80-81	3591.0
82-83	3665.0
84-85	4091.0
86-87	4363.0
88-89	5255.0
90-91	14249.0
92-93	9589.0
94-95	34942.0
96-97	596138.0
98-99	7268056.0
100-101	7.2924052E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.004838464944324
#Duplication Level	Percentage of deduplicated	Percentage of total
1	48.18289422265824	8.193423330298026
2	17.270211393681183	5.87354310009979
3	9.92296865267256	5.0621543709420935
4	6.193213372024435	4.2125837188083475
5	3.9648829895957034	3.371109738524024
6	2.590043142060492	2.6425959148785307
7	1.8909095265423683	2.2508227735474127
8	1.415554727170953	1.9257023579064312
9	1.1052303742133402	1.691483758204304
>10	6.408215724838352	20.32541196689328
>50	0.559347276516945	6.596882433867715
>100	0.4043972808269237	13.713025717895164
>500	0.049477165570190475	5.817980273024195
>1k	0.03892317665152966	12.875992114532927
>5k	0.002915596339412187	3.2945226086093737
>10k+	8.153786372932388E-4	2.1527658219683863
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.1743042645771375E-6	0.0	2.4697217058308547E-6	0.0	1.2348608529154274E-6
2	6.1743042645771375E-6	0.0	2.4697217058308547E-6	0.0	1.2348608529154274E-6
3	7.409165117492565E-6	1.2348608529154274E-6	6.1743042645771375E-6	0.0	1.2348608529154274E-6
4	1.1113747676238847E-5	1.2348608529154274E-6	1.1113747676238847E-5	0.0	4.9394434116617095E-6
5	1.7288051940815985E-5	1.2348608529154274E-6	1.1113747676238847E-5	0.0	1.1113747676238847E-5
6	3.457610388163197E-5	1.2348608529154274E-6	1.6053191087900556E-5	1.2348608529154274E-6	1.1113747676238847E-5
7	3.457610388163197E-5	1.2348608529154274E-6	1.9757773646646838E-5	1.2348608529154274E-6	1.2348608529154275E-5
8	3.828068644037825E-5	1.2348608529154274E-6	2.0992634499562268E-5	1.2348608529154274E-6	1.2348608529154275E-5
9	4.0750408146209105E-5	2.4697217058308547E-6	2.0992634499562268E-5	1.2348608529154274E-6	1.481833023498513E-5
10-11	6.29779034986868E-5	4.9394434116617095E-6	2.6549508337681688E-5	1.8522912793731412E-6	2.3462356205393124E-5
12-13	9.384942482157248E-5	6.1743042645771375E-6	3.8898116866835964E-5	2.4697217058308547E-6	2.8401799617054832E-5
14-15	1.2225122443862733E-4	6.1743042645771375E-6	5.3099016675363375E-5	4.322012985203996E-6	2.8401799617054832E-5
16-17	1.481833023498513E-4	6.1743042645771375E-6	5.927332093994052E-5	6.1743042645771375E-6	2.8401799617054832E-5
18-19	1.6053191087900558E-4	6.1743042645771375E-6	6.668248605743308E-5	6.1743042645771375E-6	2.8401799617054832E-5
20-21	1.6423649343775184E-4	6.1743042645771375E-6	8.026595543950279E-5	6.1743042645771375E-6	3.457610388163197E-5
22-23	1.8090711495211012E-4	6.1743042645771375E-6	9.01448422628262E-5	6.1743042645771375E-6	3.457610388163197E-5
24-25	2.056043320104187E-4	6.1743042645771375E-6	9.446685524803019E-5	1.2348608529154275E-5	3.457610388163197E-5
26-27	2.2412724480415009E-4	6.1743042645771375E-6	1.0002372908614962E-4	1.2348608529154275E-5	3.58109647345474E-5
28-29	2.4141529674496606E-4	7.409165117492565E-6	1.0681546377718448E-4	1.6053191087900556E-5	4.0750408146209105E-5
30-31	2.6364279209744376E-4	8.644025970407993E-6	1.0928518548301532E-4	1.7905482367273697E-5	4.2602699425582247E-5
32-33	2.8154827446471746E-4	8.644025970407993E-6	1.1484205932113475E-4	1.9757773646646838E-5	4.322012985203996E-5
34-35	2.9389688299387174E-4	1.1113747676238847E-5	1.4200899808527415E-4	1.9757773646646838E-5	4.692471241078624E-5
36-37	3.2970784772841914E-4	1.1113747676238847E-5	1.518878849085976E-4	1.9757773646646838E-5	4.815957326370167E-5
38-39	3.698408254481705E-4	1.1113747676238847E-5	1.5867961959963243E-4	1.9757773646646838E-5	6.359533392514451E-5
40-41	4.000949163445985E-4	1.3583469382069701E-5	1.7411538026107525E-4	1.9757773646646838E-5	6.853477733680623E-5
42-43	4.315838680939419E-4	1.3583469382069701E-5	1.9202086262834895E-4	1.9757773646646838E-5	7.532651202784107E-5
44-45	4.581333764316236E-4	1.3583469382069701E-5	1.9696030604001067E-4	1.9757773646646838E-5	8.026595543950279E-5
46-47	5.266681537684297E-4	1.5435760661442844E-5	2.0683919286333408E-4	2.2227495352477694E-5	9.076227268928392E-5
48-49	5.705057140469275E-4	1.7288051940815985E-5	2.2227495352477694E-4	2.5932077911223976E-5	9.570171610094563E-5
50-51	6.112561221931366E-4	1.7288051940815985E-5	2.3277127077455807E-4	2.5932077911223976E-5	9.817143780677649E-5
52-53	6.606505563097537E-4	1.7288051940815985E-5	2.642602225239014E-4	2.5932077911223976E-5	9.87888682332342E-5
54-55	7.081926991469976E-4	1.7288051940815985E-5	2.858702874499214E-4	2.5932077911223976E-5	9.87888682332342E-5
56-57	7.544999811313262E-4	1.7288051940815985E-5	2.963666046997026E-4	2.7166938764139402E-5	1.166943506005079E-4
58-59	7.859889328806695E-4	1.7288051940815985E-5	2.9945375683199116E-4	2.9019230043512547E-5	1.3336497211486616E-4
60-61	8.353833669972866E-4	1.7288051940815985E-5	3.155069479198917E-4	3.087152132288569E-5	1.4262642851173188E-4
62-63	8.761337751434958E-4	1.7288051940815985E-5	3.414390258311157E-4	3.14889517493434E-5	1.4324385893818957E-4
64-65	9.212061962749088E-4	1.7288051940815985E-5	3.605793690513048E-4	3.210638217580111E-5	1.5435760661442843E-4
66-67	9.687483391121528E-4	1.7288051940815985E-5	3.6181422990422024E-4	3.334124302871654E-5	1.6300163258483642E-4
68-69	0.0010267867991991778	2.0992634499562268E-5	3.642839516100511E-4	3.457610388163197E-5	1.7658510196690613E-4
70-71	0.0010872949809920339	2.0992634499562268E-5	3.6675367331588193E-4	3.457610388163197E-5	1.839942670843987E-4
72-73	0.0011718829494167405	2.0992634499562268E-5	3.840417252566979E-4	3.457610388163197E-5	1.895511409225181E-4
74-75	0.0012447397397387509	2.0992634499562268E-5	3.840417252566979E-4	3.457610388163197E-5	1.981951668929261E-4
76-77	0.0013095699345168107	2.0992634499562268E-5	3.840417252566979E-4	3.457610388163197E-5	2.018997494516724E-4
78-79	0.0013663735337509204	2.0992634499562268E-5	3.8465915568315563E-4	3.457610388163197E-5	2.0992634499562266E-4
80-81	0.0014330560198083536	2.0992634499562268E-5	3.8898116866835966E-4	3.457610388163197E-5	2.161006492601998E-4
82-83	0.0014725715671016473	2.0992634499562268E-5	3.9021602952127505E-4	3.58109647345474E-5	2.198052318189461E-4
84-85	0.001512087114394941	2.2227495352477694E-5	3.970077642123099E-4	3.704582558746282E-5	2.2844925778935408E-4
86-87	0.001520731140365349	2.2227495352477694E-5	4.050343597562602E-4	3.704582558746282E-5	2.3524099248038895E-4
88-89	0.0015238182924976374	2.3462356205393124E-5	4.050343597562602E-4	3.9515547293293676E-5	2.4018043589205064E-4
90	0.0015238182924976374	2.469721705830855E-5	4.050343597562602E-4	3.9515547293293676E-5	2.432675880243392E-4
>>END_MODULE
