##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	SRR21290306_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	88520848
Sequences flagged as poor quality	0
Sequence length	35-101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.04817278749973	37.0	37.0	37.0	37.0	37.0
2	36.16979789890851	37.0	37.0	37.0	37.0	37.0
3	36.29374043050288	37.0	37.0	37.0	37.0	37.0
4	36.32763869365553	37.0	37.0	37.0	37.0	37.0
5	36.34430606674712	37.0	37.0	37.0	37.0	37.0
6	36.309330091370114	37.0	37.0	37.0	37.0	37.0
7	36.278153164551696	37.0	37.0	37.0	37.0	37.0
8	36.33835238451398	37.0	37.0	37.0	37.0	37.0
9	36.320665737409115	37.0	37.0	37.0	37.0	37.0
10-11	36.34091884207888	37.0	37.0	37.0	37.0	37.0
12-13	36.321556233849	37.0	37.0	37.0	37.0	37.0
14-15	36.31827637936772	37.0	37.0	37.0	37.0	37.0
16-17	36.31844954196553	37.0	37.0	37.0	37.0	37.0
18-19	36.31527596753253	37.0	37.0	37.0	37.0	37.0
20-21	36.30901905729597	37.0	37.0	37.0	37.0	37.0
22-23	36.29640082639064	37.0	37.0	37.0	37.0	37.0
24-25	36.26498643573771	37.0	37.0	37.0	37.0	37.0
26-27	36.22149604237863	37.0	37.0	37.0	37.0	37.0
28-29	36.20692448630858	37.0	37.0	37.0	37.0	37.0
30-31	36.19755106164369	37.0	37.0	37.0	37.0	37.0
32-33	36.193386844870716	37.0	37.0	37.0	37.0	37.0
34-35	36.179399857308184	37.0	37.0	37.0	37.0	37.0
36-37	36.195155889296046	37.0	37.0	37.0	37.0	37.0
38-39	36.19159946645643	37.0	37.0	37.0	37.0	37.0
40-41	36.19276677422026	37.0	37.0	37.0	37.0	37.0
42-43	36.17658445691377	37.0	37.0	37.0	37.0	37.0
44-45	36.174412459869686	37.0	37.0	37.0	37.0	37.0
46-47	36.17148682393464	37.0	37.0	37.0	37.0	37.0
48-49	36.14667835507498	37.0	37.0	37.0	37.0	37.0
50-51	36.142382940627144	37.0	37.0	37.0	37.0	37.0
52-53	36.14492522746784	37.0	37.0	37.0	37.0	37.0
54-55	36.13534450259036	37.0	37.0	37.0	37.0	37.0
56-57	36.11990631728597	37.0	37.0	37.0	37.0	37.0
58-59	36.10713536937297	37.0	37.0	37.0	37.0	37.0
60-61	36.104610644712565	37.0	37.0	37.0	37.0	37.0
62-63	36.09591554850274	37.0	37.0	37.0	37.0	37.0
64-65	36.080833591342525	37.0	37.0	37.0	37.0	37.0
66-67	36.08052754448207	37.0	37.0	37.0	37.0	37.0
68-69	36.06323974175977	37.0	37.0	37.0	37.0	37.0
70-71	36.04686273226643	37.0	37.0	37.0	37.0	37.0
72-73	36.024346515318484	37.0	37.0	37.0	37.0	37.0
74-75	36.021586221612324	37.0	37.0	37.0	37.0	37.0
76-77	36.00986249657795	37.0	37.0	37.0	37.0	37.0
78-79	35.99695439857962	37.0	37.0	37.0	37.0	37.0
80-81	35.96875100953196	37.0	37.0	37.0	37.0	37.0
82-83	35.981872032898124	37.0	37.0	37.0	37.0	37.0
84-85	35.966865562429874	37.0	37.0	37.0	37.0	37.0
86-87	35.98013913151456	37.0	37.0	37.0	37.0	37.0
88-89	35.956867299124	37.0	37.0	37.0	37.0	37.0
90-91	35.937832228428135	37.0	37.0	37.0	37.0	37.0
92-93	35.928271482436216	37.0	37.0	37.0	37.0	37.0
94-95	35.90215169693499	37.0	37.0	37.0	37.0	37.0
96-97	35.906160627935144	37.0	37.0	37.0	37.0	37.0
98-99	35.88643614164351	37.0	37.0	37.0	37.0	37.0
100-101	35.86006271072689	37.0	37.0	37.0	37.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	66750.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	9.0
15	72.0
16	395.0
17	1022.0
18	1820.0
19	2717.0
20	3853.0
21	6940.0
22	13316.0
23	27566.0
24	54333.0
25	97481.0
26	158307.0
27	253897.0
28	383263.0
29	566972.0
30	774491.0
31	1062542.0
32	1408252.0
33	2034985.0
34	3248994.0
35	7677143.0
36	5.2947586E7
37	1.7728142E7
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.49889978765697	15.637119223534606	12.244382966044363	45.61959802276406
2	22.118476596637453	19.30335978803256	32.13779339863488	26.440370216695108
3	23.66051878132867	19.96467415832366	23.390036969887397	32.98477009046027
4	25.000658816289768	25.303040513770664	20.721790874197723	28.974509795741852
5	25.948715237764468	29.18794658981835	22.953001224782522	21.910336947634665
6	21.32600119894954	33.82980401880306	23.010394611677572	21.833800170569827
7	18.770961860919094	21.74502644298063	38.27741593159426	21.206595764506016
8	19.977144529810253	23.97377790229685	29.23046030043741	26.81861726745549
9	20.93370191773028	22.04000115449712	31.480983859910978	25.54531306786163
10-11	22.80509377869638	30.45915577591442	23.936867232539075	22.798883212850125
12-13	22.09182650426172	25.608338667880904	27.562396626801178	24.737438201056197
14-15	22.128859957847023	26.204148347825047	27.028092338606243	24.63889935572169
16-17	23.002389539065796	26.221633610525537	26.448280002342685	24.327696848065987
18-19	22.787876909825	26.63296614250521	25.934578181931485	24.64457876573831
20-21	22.81166305087328	26.40056432899074	26.223132145734784	24.564640474401198
22-23	22.691474720166802	26.54730876308925	26.332417121737432	24.42879939500652
24-25	22.727886793832155	26.30788861702758	26.45318969486892	24.51103489427134
26-27	22.856500267059545	26.55864806618516	26.284107090128018	24.30074457662728
28-29	22.682908280619387	26.345485236601675	26.354577025360744	24.617029457418194
30-31	22.72717576882859	26.0202802804599	26.529419529695463	24.723124421016042
32-33	22.9374553085642	26.257001322586927	26.335226476551444	24.470316892297433
34-35	22.91623062600638	26.341897599237463	26.2012583198452	24.540613454910954
36-37	22.636451825520034	26.279117227957943	26.430740434640832	24.65369051188119
38-39	23.026201223545456	26.387471417249763	26.423646828250547	24.162680530954233
40-41	23.166840791455588	26.271827491212157	25.993375404297826	24.567956313034433
42-43	22.925289098455217	26.349794754557866	26.211401589705773	24.51351455728114
44-45	23.07564046109665	26.256424755142245	26.3748012618029	24.293133521958215
46-47	22.990609900637292	26.01128851216258	26.392296521939425	24.605805065260704
48-49	22.802389639573644	25.82986241057521	26.54960588282375	24.818142067027395
50-51	23.040139413465283	25.824954122687906	26.404482608332163	24.73042385551465
52-53	23.28047182718894	26.143498146601374	26.063019257370534	24.513010768839152
54-55	22.823300462784548	26.111384021484547	26.383849707941604	24.681465807789305
56-57	22.945952850953567	26.27699490764492	26.28571033990831	24.491341901493207
58-59	22.93589283777993	26.192816142653392	26.228741972769313	24.642549046797363
60-61	23.012118706783845	26.04110089245929	26.218717373428156	24.72806302732871
62-63	23.120132352316215	25.889378428677762	26.290077468586624	24.700411750419402
64-65	23.13651491553089	26.120125601922485	26.000073956944362	24.743285525602264
66-67	23.216738994824997	26.025537482127376	26.09341118314091	24.664312339906715
68-69	23.148492352770468	26.135962517249766	26.316955616731946	24.398589513247813
70-71	23.538561792377283	26.0712022228235	26.092676906580998	24.29755907821822
72-73	23.288914276407652	25.94311774345269	26.07321149292839	24.694756487211272
74-75	23.383693704471266	26.103779280393013	26.096578110339212	24.415948904796505
76-77	23.34324454363361	26.09948110938425	26.096424033091008	24.460850313891136
78-79	23.120820808779687	26.176682818093973	25.92805685896502	24.774439514161323
80-81	23.351742837233004	26.153613045780855	25.968395927971844	24.5262481890143
82-83	23.41429114190985	26.230680310785214	25.717317204663903	24.63771134264103
84-85	23.295135392191117	26.26006093443142	25.84598848458531	24.59881518879216
86-87	23.272483822151692	26.211244224102714	25.882828642499	24.633443311246594
88-89	23.42847898675805	25.954031655933925	25.83880420312373	24.7786851541843
90-91	23.33649276622407	25.966514651923795	26.136216740854028	24.560775840998105
92-93	23.656188251092207	25.891063333756627	26.059347666917027	24.393400748234136
94-95	23.455474042821383	25.97770431632467	26.07917400751421	24.487647633339733
96-97	23.471866166906725	25.750467536644976	26.12914718211202	24.648519114336274
98-99	23.652432452562792	24.964051379245454	26.54942140324949	24.834094764942265
100-101	24.54150238437674	11.57209366758471	32.40783441791217	31.478569530126382
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	66867.0
1	33460.0
2	60.0
3	74.5
4	84.5
5	101.0
6	118.0
7	124.0
8	146.0
9	160.0
10	210.0
11	281.5
12	337.5
13	433.5
14	588.0
15	825.0
16	1254.0
17	1844.5
18	2628.5
19	3707.0
20	4894.0
21	7307.0
22	11669.5
23	17186.0
24	22978.5
25	30711.0
26	39346.5
27	53807.0
28	74218.5
29	96862.5
30	133431.0
31	180287.0
32	261477.0
33	378395.0
34	507484.0
35	662198.5
36	902894.0
37	1241039.5
38	1646428.0
39	2208501.5
40	2762249.0
41	3239854.5
42	3781153.5
43	4348621.0
44	4804321.0
45	5127416.5
46	5295885.5
47	5311060.0
48	5189327.0
49	5019846.5
50	4759344.0
51	4424574.5
52	3954887.5
53	3289972.0
54	2758191.0
55	2411675.0
56	2126588.5
57	1821747.5
58	1531071.5
59	1230742.5
60	1009826.0
61	901130.0
62	844449.5
63	779729.0
64	650630.5
65	529364.0
66	464577.0
67	414899.5
68	339358.0
69	257812.5
70	188508.0
71	137033.5
72	105020.0
73	82560.0
74	70558.0
75	52728.5
76	32452.5
77	28178.5
78	21265.0
79	14061.0
80	12668.5
81	8802.5
82	3390.0
83	468.5
84	312.0
85	231.5
86	175.5
87	152.5
88	125.5
89	93.5
90	93.0
91	86.0
92	77.5
93	84.0
94	91.5
95	143.5
96	336.5
97	956.0
98	2819.0
99	3298.5
100	1300.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.11164149715330338
2	0.08536520120096454
3	0.07717955887634516
4	0.0754070950608155
5	0.07543646667280006
6	0.07540596538343149
7	0.07540596538343149
8	0.07540596538343149
9	0.0754070950608155
10-11	0.07540596538343149
12-13	0.07542573473765185
14-15	0.07550537699322538
16-17	0.07541669731857968
18-19	0.07561156666732338
20-21	0.07542968860849593
22-23	0.07643792567373507
24-25	0.0810487039166186
26-27	0.08497037895524905
28-29	0.08857348497158546
30-31	0.09138807617387489
32-33	0.09396938899636388
34-35	0.09645580891859508
36-37	1.3343362641935144E-4
38-39	1.8658482053780055E-5
40-41	2.092064049049742E-5
42-43	3.2286394500352525E-4
44-45	8.142481253449493E-5
46-47	1.7642474773720842E-4
48-49	1.4136968176304868E-4
50-51	6.8990092378073E-5
52-53	1.0914258478888731E-4
54-55	3.280013082501835E-5
56-57	0.0
58-59	5.655473508082413E-7
60-61	0.0
62-63	4.52461780638226E-6
64-65	2.8279650609893935E-6
66-67	3.959266075146281E-6
68-69	0.0
70-71	1.1312884769947993E-6
72-73	3.959650281198241E-6
74-75	0.0
76-77	2.2628247402412965E-6
78-79	0.0
80-81	5.65754019815263E-7
82-83	5.092032201875857E-5
84-85	7.355553895840831E-6
86-87	6.224278381778608E-6
88-89	2.467221547056106E-4
90-91	4.6971556702074035E-5
92-93	6.2262748504355384E-6
94-95	5.887418618679464E-4
96-97	2.267070532435287E-5
98-99	1.9603643503199462E-5
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
34-35	86925.0
36-37	1806.0
38-39	2209.0
40-41	2003.0
42-43	2262.0
44-45	2158.0
46-47	2195.0
48-49	2267.0
50-51	2047.0
52-53	2173.0
54-55	2099.0
56-57	2180.0
58-59	2334.0
60-61	2268.0
62-63	2506.0
64-65	2533.0
66-67	2630.0
68-69	2722.0
70-71	3084.0
72-73	3243.0
74-75	3239.0
76-77	3495.0
78-79	3802.0
80-81	4177.0
82-83	4690.0
84-85	4884.0
86-87	5041.0
88-89	5996.0
90-91	15335.0
92-93	10995.0
94-95	33253.0
96-97	636003.0
98-99	7935394.0
100-101	7.97229E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.596034198453037
#Duplication Level	Percentage of deduplicated	Percentage of total
1	56.261691127522454	10.462443322702093
2	16.862815845342812	6.271630002844216
3	8.084792673261946	4.510352431181451
4	4.700864657098759	3.4966975970283096
5	2.851626555733218	2.6514472475815887
6	2.0704234963511037	2.31009996860555
7	1.4071578319947207	1.8317288616476401
8	1.0186060609351293	1.5153626515120961
9	0.8277418861764854	1.385344478054695
>10	4.984729460497386	17.455055595022518
>50	0.4757381324779551	6.1645771813060435
>100	0.36270686030836036	13.620393381551702
>500	0.046484408214575376	6.004146197484141
>1k	0.040165963498224315	14.865942116144199
>5k	0.0033721230287403967	4.196605504490748
>10k+	0.0010829175580101273	3.2581734628430254
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.1296773840214455E-6	0.0	2.259354768042891E-6	0.0	1.1296773840214455E-6
2	1.1296773840214455E-6	0.0	5.6483869201072276E-6	0.0	4.518709536085782E-6
3	9.037419072171564E-6	0.0	6.778064304128672E-6	0.0	1.468580599227879E-5
4	1.016709645619301E-5	0.0	1.9204515528364573E-5	1.1296773840214455E-6	2.3723225064450353E-5
5	3.0501289368579027E-5	0.0	2.5982579832493244E-5	2.259354768042891E-6	2.8241934600536135E-5
6	5.309483704900794E-5	0.0	2.937161198455758E-5	2.259354768042891E-6	2.8241934600536135E-5
7	5.309483704900794E-5	1.1296773840214455E-6	3.5019998904664806E-5	2.259354768042891E-6	2.8241934600536135E-5
8	5.761354658509372E-5	1.1296773840214455E-6	3.5019998904664806E-5	2.259354768042891E-6	2.937161198455758E-5
9	6.21322561211795E-5	1.1296773840214455E-6	3.953870844075059E-5	2.259354768042891E-6	3.6149676288686255E-5
10-11	7.003999780932961E-5	3.953870844075059E-6	4.292774059281493E-5	2.259354768042891E-6	3.8409031056729146E-5
12-13	8.416096510959769E-5	4.518709536085782E-6	4.9705804896943597E-5	2.259354768042891E-6	4.123322451678276E-5
14-15	1.118380610181231E-4	6.778064304128672E-6	6.439161088922239E-5	2.259354768042891E-6	4.292774059281493E-5
16-17	1.316074152384984E-4	6.778064304128672E-6	7.455870734541541E-5	2.259354768042891E-6	4.46222566688471E-5
18-19	1.468580599227879E-4	6.778064304128672E-6	7.907741688150118E-5	3.389032152064336E-6	4.7446450128900705E-5
20-21	1.542009629189273E-4	6.778064304128672E-6	8.246644903356552E-5	4.518709536085782E-6	5.365967574101866E-5
22-23	1.6267354329908812E-4	7.342902996139395E-6	8.980935202970491E-5	5.6483869201072276E-6	5.987290135313661E-5
24-25	1.7453515583131332E-4	7.907741688150118E-6	9.093902941372637E-5	5.6483869201072276E-6	5.987290135313661E-5
26-27	1.9769354220375296E-4	8.472580380160841E-6	9.319838418176925E-5	6.2132256121179496E-6	5.987290135313661E-5
28-29	2.2198160596021403E-4	9.037419072171564E-6	1.0054128717790865E-4	6.778064304128672E-6	6.495644958123312E-5
30-31	2.4683450840868584E-4	9.037419072171564E-6	1.0336548063796225E-4	6.778064304128672E-6	7.173451388536178E-5
32-33	2.6886321739710405E-4	9.037419072171564E-6	1.0788419017404804E-4	6.778064304128672E-6	8.133677164954406E-5
34-35	2.7733579777726485E-4	9.037419072171564E-6	1.2087548009029466E-4	6.778064304128672E-6	8.529064249361913E-5
36-37	2.948457972295973E-4	9.037419072171564E-6	1.2313483485833755E-4	6.778064304128672E-6	9.037419072171563E-5
38-39	3.242174092141549E-4	9.037419072171564E-6	1.3047773785447694E-4	7.907741688150118E-6	1.0054128717790865E-4
40-41	3.389032152064336E-4	9.037419072171564E-6	1.4233935038670214E-4	9.037419072171564E-6	1.1127322232611238E-4
42-43	3.609319241948518E-4	9.037419072171564E-6	1.5984934983903453E-4	9.037419072171564E-6	1.1522709317018744E-4
44-45	3.801364397232164E-4	9.037419072171564E-6	1.6323838199109887E-4	1.1296773840214453E-5	1.2087548009029466E-4
46-47	4.089432130157633E-4	9.037419072171564E-6	1.7058128498723826E-4	1.468580599227879E-5	1.2934806047045552E-4
48-49	4.343609541562458E-4	9.037419072171564E-6	1.841374135954956E-4	1.468580599227879E-5	1.440338664627343E-4
50-51	4.6542708221683553E-4	9.602257764182287E-6	1.892209618235921E-4	1.468580599227879E-5	1.5024709207485225E-4
52-53	5.100493388856826E-4	1.016709645619301E-5	2.0051773566380656E-4	1.5815483376300236E-5	1.5307128553490584E-4
54-55	5.484583699424117E-4	1.016709645619301E-5	2.0503644519989233E-4	1.6945160760321682E-5	1.5984934983903453E-4
56-57	5.789596593109908E-4	1.016709645619301E-5	2.1689805773211752E-4	1.6945160760321682E-5	1.694516076032168E-4
58-59	6.275357868239129E-4	1.016709645619301E-5	2.265003154962998E-4	1.6945160760321682E-5	1.7735934929136691E-4
60-61	6.727228821847707E-4	1.016709645619301E-5	2.4965870186873944E-4	1.6945160760321682E-5	1.81313220135442E-4
62-63	7.122615906255214E-4	1.016709645619301E-5	2.807248299293292E-4	1.6945160760321682E-5	1.8357257490348488E-4
64-65	7.534948151423041E-4	1.016709645619301E-5	2.9145676507753295E-4	1.7509999452332403E-5	1.8978580051560285E-4
66-67	8.116732004194086E-4	1.016709645619301E-5	2.937161198455758E-4	1.8074838144343128E-5	1.931748326676672E-4
68-69	8.692867470045022E-4	1.016709645619301E-5	2.959754746136187E-4	1.9769354220375294E-5	1.9825838089576367E-4
70-71	9.161683584413922E-4	1.1861612532225178E-5	2.976699906896509E-4	2.033419291238602E-5	2.1294418688804246E-4
72-73	9.946809366308826E-4	1.2426451224235899E-5	3.05577732377801E-4	2.0899031604396743E-5	2.2706515418831054E-4
74-75	0.001053424160599998	1.2991289916246622E-5	3.0727224845383317E-4	2.1463870296407465E-5	2.310190250323856E-4
76-77	0.0010986112559608556	1.3556128608257345E-5	3.084019258378546E-4	2.1463870296407465E-5	2.3779708933651425E-4
78-79	0.0011319367387894884	1.4120967300268068E-5	3.0953160322187603E-4	2.1463870296407465E-5	2.462696697166751E-4
80-81	0.001173169963306271	1.468580599227879E-5	3.106612806058975E-4	2.1463870296407465E-5	2.553070887888467E-4
82-83	0.0012110141556709894	1.468580599227879E-5	3.112261192979082E-4	2.1463870296407465E-5	2.6152031440096463E-4
84-85	0.0012375615741954935	1.468580599227879E-5	3.1913386098605836E-4	2.1463870296407465E-5	2.711225721651469E-4
86-87	0.0012499880254197293	1.5250644684289513E-5	3.270416026742084E-4	2.1463870296407465E-5	2.767709590852541E-4
88-89	0.0012562012510318474	1.5815483376300236E-5	3.3212515090230493E-4	2.1463870296407465E-5	2.818545073133506E-4
90	0.0012562012510318474	1.6945160760321682E-5	3.3438450567034784E-4	2.1463870296407465E-5	2.8241934600536136E-4
>>END_MODULE
